1. One in, one out: Generic circumscription within subtribe Manilkarinae (Sapotaceae)
- Author
-
Aina Randriarisoa, Yamama Naciri, Kate Armstrong, Carlos Galan Boluda, Stephanie Dafreville, Charles Pouchon, Laurent Gautier, Conservatoire et Jardin Botaniques de Genève (CJBG), Université de Genève = University of Geneva (UNIGE), New York Botanical Garden (NYBG), Institute of Systematic Botany, Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), AR was funded by the Swiss Government Excellence Ph.D. scholarship from September 2018 to August 2021 and by the University of Geneva from September to December 2021. This work is part of a project led by LG on the Malagasy Sapotaceae, financially supported by the Franklinia foundation (grant No. 2019-20) and formerly supported by a grant attributed to YN and LG by the Swiss National Foundation (grant no. 31003A_166349/1) and by two grants attributed to YN by the Fondation Ernst et Lucie Schmidheiny. We would like to express our gratitude to Prof. Roman Ulm and Martine Mir for financial support and fund management, respectively. We thank the curators of the herbaria G, MO, P, TAN and TEF for allowing us to study their specimens and to perform limited de-structive sampling. We also thank people at the iGE3 platform for their help with the sequencing process (https://ige3.genomics.unige.ch). Some computations were performed at the University of Geneva on the Bao-bab/Yggdrasil cluster. We are grateful to Camille Christe for her precious advice on the bioinformatics analyses, to Regine Niba for her help in the lab, to Romain Dewaele for his help with the figures and to Ulf Swenson for fruitful discussions at the inception of this project. We are grateful to the Malagasy government for providing us with a research permit to collect samples. We would also like to thank Richard Randrianaivo from the MBG, Patrick Ranirison and Jacquis Tahinarivony from the University of Antananarivo (DBEV) for facilitating the fieldtrips in Madagascar, and as well as the Malagasy people on site for their help collecting Sapotaceae specimens. SD would like to thank the National Parks and Conservation Service (NPCS) of Mauritius for permission to sample in the national park.
- Subjects
ancestral state reconstruction ,[SDV]Life Sciences [q-bio] ,Madagascar ,target gene capture ,Plant Science ,Pacific Islands ,ancestral state reconstruction integrative taxonomy Madagascar Pacific Islands target gene capture ,integrative taxonomy ,Ecology, Evolution, Behavior and Systematics - Abstract
International audience; Previous phylogenetic studies have demonstrated that the Manilkarinae are a monophyletic subtribe if Northia is excluded. The subtribe consists of four genera: Faucherea, Labourdonnaisia, Labramia and Manilkara. However, the same phylogenetic studies also raised taxonomic issues concerning unclear generic delimitations and unresolved relationships. The current study's aims are: to resolve these taxonomic issues using a molecular phylogeny based on hundreds of nuclear markers sequenced from a representative sampling of taxa across the four genera; to find relevant morphological characters allowing the distinction of the clades retrieved with the phylogeny; and finally to understand the evolutionary history of the subtribe by conducting a divergence time estimation and ancestral state reconstructions. Our phylogeny shows a well-resolved backbone with four main lineages: the Labramia clade, the main clade of Manilkara, a clade in which all species of Labourdonnaisia and Faucherea are mixed, and a clade of three Pacific Manilkara species. The main clade of Manilkara is retrieved as sister to Labramia, and the Labourdonnaisia-Faucherea clade is clearly assessed as sister to the three Pacific Manilkara species. As a consequence, Faucherea is synonymized with Labourdonnaisia, and the three Pacific Manilkara are considered to be a separate genus, for which the name Abebaia is resurrected. We provide emended descriptions for Labourdonnaisia and Abebaia as well as the necessary new combinations. The ancestral state reconstruction of flower characters shows that ancestral Manilkarinae were characterized by a hexamerous corolla, well-developed dorsal appendages and staminodes, and a pubescent ovary. These character states have been retained in the main Manilkara clade, but surprisingly also in Abebaia, which appears as a cryptic genus. The lack of dorsal appendages and the reduction of staminodes observed in Labourdonnaisia appeared after the split from Abebaia. The increase in corolla merism observed mainly in the Mascarene Labourdonnaisia, which was used to separate it from Faucherea, appears to be a derived state, which evolved separately in a few species during the radiation of Labourdonnaisia on Madagascar and the Mascarenes. The glabrous ovary state observed in Labramia also constitutes a derived synapomorphic state in the genus.
- Published
- 2022
- Full Text
- View/download PDF