28 results on '"Eroğlu, İpek"'
Search Results
2. Production of 5-fluorouracil-loaded PLGA nanoparticles with toroidal microfluidic system and optimization of process variables by design of experiments
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Turkmen Koc, Seyma Nur, Conger, Elif, Ozturk, Sukru, Eroglu, Ipek, and Ulubayram, Kezban
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- 2024
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3. CB65 and novel CB65 liposomal system suppress MG63 and Saos-2 osteosarcoma cell growth in vitro.
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Zorba, Başak Işıl, Boyacıoğlu, Özge, Çağlayan, Tuğba, Reçber, Tuba, Nemutlu, Emirhan, Eroğlu, İpek, and Korkusuz, Petek
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CANNABINOID receptors ,TRPV cation channels ,CELL growth ,OSTEOSARCOMA - Abstract
Curable approaches for primary osteosarcoma are inadequate and urge investigation of novel therapeutic formulations. Cannabinoid ligands exert antiproliferative and apoptotic effect on osteosarcoma cells via cannabinoid 2 (CB2) or transient receptor potential vanilloid type (TRPV1) receptors. In this study, we confirmed CB2 receptor expression in MG63 and Saos-2 osteosarcoma cells by qRT-PCR and flow cytometry (FCM), then reported the reduction effect of synthetic specific CB2 receptor agonist CB65 on the proliferation of osteosarcoma cells by WST-1 (water-soluble tetrazolium-1) and RTCA (real-time impedance-based proliferation). CB65 revealed an IC50 (inhibitory concentration) for MG63 and Saos-2 cells as 1.11 × 10
−11 and 4.95 × 10−11 M, respectively. The specific antiproliferative effect of CB65 on osteosarcoma cells was inhibited by CB2 antagonist AM630. CB65 induced late apoptosis of MG63 and Saos-2 cells at 24 and 48 h, respectively by FCM when applied submaximal concentration. A novel CB65 liposomal system was generated by a thin film hydration method with optimal particle size (141.7 ± 0.6 nm), polydispersity index (0.451 ± 0.026), and zeta potential (−10.9 ± 0.3 mV) values. The encapsulation efficiency (EE%) of the CB65-loaded liposomal formulation was 51.12%. The CB65 and CB65-loaded liposomal formulation releasing IC50 of CB65 reduced proliferation by RTCA and invasion by scratch assay and induced late apoptosis of MG63 and Saos-2 cells, by FCM. Our results demonstrate the CB2 receptor-mediated antiproliferative and apoptotic effect of a new liposomal CB65 delivery system on osteosarcoma cells that can be used as a targeted and intelligent tool for bone tumors to ameliorate pediatric bone cancers following in vivo validation. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. CB65 and novel CB65 liposomal system suppress MG63 and Saos-2 osteosarcoma cell growth in vitro
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Zorba, Başak Işıl, primary, Boyacıoğlu, Özge, additional, Çağlayan, Tuğba, additional, Reçber, Tuba, additional, Nemutlu, Emirhan, additional, Eroğlu, İpek, additional, and Korkusuz, Petek, additional
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- 2023
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5. Polymeric Nanoparticle Versus Liposome Formulations: Comparative Physicochemical and Metabolomic Studies as l-Carnitine Delivery Systems
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Yaşacan, Merve, Erikçi, Açelya, Eylem, Cemil Can, Çiftçi, Samiye Yabanoğlu, Nemutlu, Emirhan, Ulubayram, Kezban, and Eroğlu, İpek
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- 2020
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6. Formulation and Evaluation of Organogels Containing Hyaluronan Microparticles for Topical Delivery of Caffeine
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Simsolo, Erol Eli, Eroğlu, İpek, Tanrıverdi, Sakine Tuncay, and Özer, Özgen
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- 2018
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7. Evaluation of characteristics and in vitro antioxidant properties of RSV loaded hyaluronic acid–DPPC microparticles as a wound healing system
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Eroğlu, İpek, Gökçe, Evren H., Tsapis, Nicolas, Tanrıverdi, Sakine Tuncay, Gökçe, Göksel, Fattal, Elias, and Özer, Özgen
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- 2015
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8. The Role of Personal Knowledge Management on the Effects of Self-Leadership to Transformational Leadership: A Study on the Public Healthcare Managers
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EROĞLU, İpek and SARACEL, Nüket
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Health Care Sciences and Services ,Sağlık Bilimleri ve Hizmetleri ,Self-Leadership ,Transformational Leadership ,Personal Knowledge Management ,Healthcare Managers ,Mediating Role - Abstract
In the present era called the information age, also known as the digital age, healthcare managers have to deal with many challenges besides trying to adapt to the rapidly changing micro and macro-environment affected by the pandemic. Currently, digitalization and hybrid work ecosystem are on the rise requiring healthcare managers to effectively use self knowledge management tools to lead themselves and be able to lead their staff members by inspiring, encouraging and motivating them transforming their staff members creating value for all. However, there is insufficient research on the relationship of leadership styles and personal knowledge management. The aim of this study is to determine the mediating role of personal knowledge management in the effect of self-leadership on transformational leadership. The study was carried out with scales of self-leadership, transformational leadership and personal knowledge management. Scales were validated by factor analysis. Structural equation modeling was used to test the relationships. The research universe consisted of the public healthcare managers working in Istanbul. It was found that the validity and the reliability of the research scales were high. According to the research findings; the self-leadership and transformational leadership perceptions of healthcare managers working in public institutions are high. The results obtained demonstrate the existence of a partial mediation effect of personal knowledge management on the positive effect of self-leadership on transformational leadership.
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- 2022
9. Yeni Dünya Düzeninde Öğrenen Örgütler, Değişim ve İnovasyon Yönetimi
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EROĞLU, İpek and EROĞLU, İşıl
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Bilgi, Belge Yönetimi ,Information Science and Library Science ,Öğrenen Örgüt,Değişim Yönetimi,İnovasyon Yönetimi - Abstract
Dünyamızınkaotik ortamında bilgi çağından akıllı toplum çağına geçtiğimiz süreçte,işletmelerde insana yatırım yeni dünya düzeninin önemli bir aracıdır. Bu yapıyıoluştururken riskleri fırsata dönüştürebilen işletmeler öğrenen örgüt yapısınasahip olanlardır. Öğrenen örgüt, bilgi yaratma, bilgi edinme, bilgi transferetme ve davranışlarını yeni bilgi ve anlayışları yansıtacak şekildedeğiştirebilmede yetenekli olan organizasyondur. Öğrenen örgüt teorisi,örgütlerin nasıl öğrenmesi gerektiği, etkin ve çevre koşullarına adaptasyonkonusunda başarılı örgütleri öne çıkaran uygulamaların neler olduğu ve hangisüreçlerin örgütsel öğrenmeyi kolaylaştıracağı üzerine odaklanmaktadır. Öğrenenörgüt olabilmek için değişim yönetiminde makro düzey örgütsel hedefler ilemikro düzeyde çalışanların çıkarlarını dengeleyen değişim stratejilerineihtiyaç duyulmaktadır. Değişim olmadan inovasyon olamayacağı gibi, inovasyoniçin de ortak vizyon yaratabilen bir yönetim anlayışı, açık iletişim, sınırsızpaylaşım ve işbirliği uyumu önemlidir. Bu araştırmada, işletme ve içinde hayatbulduğu ekosistemin etkinliği, verimliliği ve sürdürülebilirliği için öğrenenörgüt olarak adlandırılan yaklaşım ile işletmelerde değişim ve inovasyonyönetimi yapmanın önemi ortaya koyulmuş ve bu alanda araştırmacıların ampirikçalışma yapmalarına ihtiyaç duyulduğu yönünde öneride bulunulmuştur.
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- 2019
10. Surface modified nanoliposome formulations provide sustained release for 5-FU and increase cytotoxicity on A431 cell line
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Yaman, Ümran, primary, Aslan, Minela, additional, Ozturk, Sukru, additional, Ulubayram, Kezban, additional, and Eroğlu, İpek, additional
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- 2020
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11. Comparative Evaluation of Clinical Efficacy and Safety of Collagen Laminin–Based Dermal Matrix Combined With Resveratrol Microparticles (Dermalix) and Standard Wound Care for Diabetic Foot Ulcers
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Çetinkalp, Şevki, primary, Gökçe, Evren Homan, additional, Şimşir, IlgınYıldırım, additional, Tuncay Tanrıverdi, Sakine, additional, Doğan, Fatma, additional, Biray Avcı, Çığır, additional, Eroğlu, İpek, additional, Utku, Tülün, additional, Gündüz, Cumhur, additional, and Özer, Özgen, additional
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- 2020
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12. A Snapshot on the Current Status of Alzheimer's Disease, Treatment Perspectives, in-Vitro and in-Vivo Research Studies and Future Opportunities
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NEMUTLU, EMİRHAN, ÖNER, LEVENT, Tezel, Gizem, Turkoglu, O. Faruk, Bozkurt, Ismail, EROĞLU, HAKAN, TİMUR, SELİN SEDA, and EROĞLU, İPEK
- Abstract
Alzheimer's Disease (AD) is one of the most challenging diseases faced by humankind. AD is still not classified as curable because of the complex structure of pathologies underlying it. As the mean life expectancy of the world population constantly increases, the prevalence of AD and treatment costs for AD also grow rapidly. Current state of the art for AD treatment mainly consists of palliative therapy aimed at providing symptomatic relief and improving the standard of living in patients with AD. However, different research groups are working on more effective and safe drug delivery options aimed at both symptomatic relief and treatment of the underlying mechanisms. In this review, the current prevalence of AD, health costs, pathologies, and available treatment options including the ones in the market and/or under trial have been reviewed. Data in the existing literature have been presented, and future opportunities have been discussed. It is our belief that these nanotechnological products provide the required efficacy and safety profiles to enable these formulations go through phase studies and enter the market after regulatory authority approval, as with cancer. Last, but not the least the metabolomic studies will be providing useful informative data on the early diagnosis of AD, thus may be clinical implications might be delayed with the administration of therapeutic agents at the initial state of the disease.
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- 2019
13. Comparative Evaluation of Clinical Efficacy and Safety of Collagen Laminin–Based Dermal Matrix Combined With Resveratrol Microparticles (Dermalix) and Standard Wound Care for Diabetic Foot Ulcers.
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Çetinkalp, Şevki, Gökçe, Evren Homan, Şimşir, IlgınYıldırım, Tuncay Tanrıverdi, Sakine, Doğan, Fatma, Biray Avcı, Çığır, Eroğlu, İpek, Utku, Tülün, Gündüz, Cumhur, and Özer, Özgen
- Abstract
This is an open, prospective, comparative parallel-arm medical device clinical study of Dermalix (Dx) in diabetic foot wounds. Dx is a 3-dimensional collagen-laminin porous-structured dermal matrix prepared and additionally impregnated with resveratrol-loaded hyaluronic acid and dipalmitoylphosphatidylcholine-based microparticles. The aim was to evaluate the efficacy and safety of Dx, an investigational medical device, in Wagner 1 and 2 wounds in comparison to a standard wound care (SWC) that consists of irrigation and cleaning with sterile saline solution. Forty-eight patients were randomized to receive either SWC or SWC + Dx. A 4-week treatment period was followed by a 2-month follow-up without treatment. The wound area measurement, total collagen, vascular epidermal growth factor, tumor necrosis factor, interleukin 1, caspase 3, glutathione, reduced/oxidized glutathione, and lipid peroxidation levels were evaluated. At the end of 4 weeks, the percentage closures of wounds were determined as 57.82% for Dx, and 26.63% for SWC groups. Dx had a significant effect on tumor necrosis factor, caspase 3, and reduced/oxidized glutathione levels. Dx provided 2 times faster wound healing and decreased oxidative stress. Application of Dx in the first phase of wound would help the wound area heal faster with a safe profile. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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14. A Snapshot on the Current Status of Alzheimer’s Disease, Treatment Perspectives, in-Vitro and in-Vivo Research Studies and Future Opportunities
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Tezel, Gizem, primary, Timur, Selin Seda, additional, Bozkurt, İsmail, additional, Türkoğlu, Ö. Faruk, additional, Eroğlu, İpek, additional, Nemutlu, Emirhan, additional, Öner, Levent, additional, and Eroğlu, Hakan, additional
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- 2019
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15. Liposome–ligand conjugates: a review on the current state of art
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Eroğlu, İpek, primary and İbrahim, Mamudu, additional
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- 2019
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16. Propolis loaded liposomes: evaluation of antimicrobial and antioxidant activities
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Aytekin, Ayşegül Aylin, primary, Tuncay Tanrıverdi, Sakine, additional, Aydın Köse, Fadime, additional, Kart, Didem, additional, Eroğlu, İpek, additional, and Özer, Özgen, additional
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- 2019
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17. In Vitro Release Test of Nano-drug Delivery Systems Based on Analytical and Technological Perspectives
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Nemutlu, Emirhan, primary, Eroğlu, İpek, additional, Eroğlu, Hakan, additional, and Kır, Sedef, additional
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- 2019
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18. Liposome-based combination therapy for acne treatment
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Eroğlu, İpek, primary, Aslan, Minela, additional, Yaman, Ümran, additional, Gultekinoglu, Merve, additional, Çalamak, Semih, additional, Kart, Didem, additional, and Ulubayram, Kezban, additional
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- 2019
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19. Liposome-based combination therapy for acne treatment.
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Eroğlu, İpek, Aslan, Minela, Yaman, Ümran, Gultekinoglu, Merve, Çalamak, Semih, Kart, Didem, and Ulubayram, Kezban
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PEPTIDE antibiotics , *ACNE , *DRUG administration , *ZETA potential , *TRETINOIN , *LIPOSOMES , *CARIOGENIC agents , *STAPHYLOCOCCUS aureus - Abstract
Acne vulgaris is one of the most common chronic diseases worldwide with the high prevalence ratio of about 80–85% in patients who are in puberty period. For the treatment options, many conventional dosage forms are available; however, existing limitations of systemic administration of drugs (oral antibiotics), such as adverse events and resistance, led for seek of new formulation options. In this study, liposomes containing tetracycline HCl and tretinoin were prepared by the film formation method. In vitro characterization studies revealed that liposomes (111.10 ± 8.02 nm; P.D.I.=0.198 ± 0.03; Z.P.=25.83 ± 0.40 mV) with an encapsulation efficiency more than 80% for both APIs were formulated. In order to maintain a suitable viscosity for topical application, optimized liposomal formulations were dispersed in carbopol-based gel. In vitro release of APIs was sustained for 24 hours with released amounts of 56.44% and 58.44% for tetracycline HCl and tretinoin, respectively. Stability evaluation of both liposomes and liposomes in hydrogels was investigated for 6 months at 4 °C and 25 °C; and no statistically significant change was observed in terms of particle size, zeta potential, encapsulation efficiency, appearance, pH, and viscosity. Cytotoxicity tests confirmed the nontoxic structure of liposomal gel formulations on mice fibroblast cells. In addition, antibacterial efficacy has been proven with Staphylococcus aureus and Streptococcus epidermidis strains as well as the effect on biofilm formation and eradication. As a result, we hereby presented a new combination drug product, which consists of dual active ingredients having comedolytic and bacteriostatic effects in a single, safe, and stable liposome formulation. [ABSTRACT FROM AUTHOR]
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- 2020
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20. Propolis loaded liposomes: evaluation of antimicrobial and antioxidant activities.
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Aytekin, Ayşegül Aylin, Tuncay Tanrıverdi, Sakine, Aydın Köse, Fadime, Kart, Didem, Eroğlu, İpek, and Özer, Özgen
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PROPOLIS ,NANOCAPSULES ,BEE products ,PARTICLE size distribution ,CHRONIC wounds & injuries ,ZETA potential ,NATURAL products - Abstract
Propolis, a natural bee product, has both antimicrobial/antifungal and antioxidant characteristics. Active substances having antimicrobial and antifungal effects are used to avoid infections, which develop during long treatment process of chronic wounds. Antioxidant substances protect wound areas against the effect of free radicals and accelerate the healing process. For this purpose, propolis was used to develop topical liposome formulations for wound treatment. Characterization studies (particle size distribution, polydispersity index, Zeta Potential, morphology pH, loading capacity, encapsulation efficiency, in-vitro release behaviour) as well as stability studies were performed. Then in-vitro antioxidant (free radical scavenging capacity and trolox equivalent antioxidant capacity) and antimicrobial/antifungal activities of formulations have been evaluated. The particle size of formulations was found within the range of 300–750 nm depending on the concentration of lipid and water phase in the formulation. The Zeta Potential and pH values of optimum formulation were −23.0 ± 0.666 and 6.34, respectively. Loading capacity and encapsulation efficiency were 66.535 ± 2.705% and 57.321 ± 2.448%. At the end of 8 h, 48.16% of propolis was released and the formulations were found stable during 3 months at +4 °C. Drug loaded liposome formulations significantly scavenged the ABTS
+ radical in a dose-dependent manner of propolis when compared with unloaded liposome formulations (p < 0.05). The minimum inhibitory concentration (MIC) values of liposomes ranged from 512 to 128 μg/mL for bacteria and 256 to 128 μg/mL for fungi. Overall results showed that effective and innovative alternative was developed for topical application in wound treatment with propolis loaded liposomal formulations having antioxidant and antimicrobial effects. [ABSTRACT FROM AUTHOR]- Published
- 2020
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21. Liposome–ligand conjugates: a review on the current state of art.
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Eroğlu, İpek and İbrahim, Mamudu
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OLIGONUCLEOTIDES , *TARGETED drug delivery , *LIPOSOMES - Abstract
Early researchers focussed on developing stimuli-responsive liposomes in order to manipulate drug release at the site of action or under certain conditions. In recent times, a great deal of efforts has been made to modify the surface of liposomes with ligands for the purpose of achieving targeted drug delivery. Due to the morphology of liposomes, their surfaces can be engineered by attaching molecules such as oligosaccharides, peptides, antibodies, antigens and oligonucleotides to the bilayer structure. Over the years, a number of techniques including the use of covalent and non-covalent linkages have been utilised in designing ligand–liposome conjugates. In this review, various strategies for the functionalisation of liposomes as well as the different types of ligand–liposome conjugates have been discussed. Finally, the pros and cons of conjugation in liposomes are concisely summarised. [ABSTRACT FROM AUTHOR]
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- 2020
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22. In VitroRelease Test of Nano-drug Delivery Systems Based on Analytical and Technological Perspectives
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Nemutlu, Emirhan, Eroğlu, İpek, Eroğlu, Hakan, and Kır, Sedef
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Background: Nanotech products are gaining more attention depending on their advantages for improving drug solubility, maintenance of drug targeting, and attenuation of drug toxicity. In vitro release test is the critical physical parameter to determine the pharmaceutical quality of the product, to monitor formulation design and batch-to-batch variation. Methods: Spectrophotometric and chromatographic methods are mostly used in quantification studies from in vitro release test of nano-drug delivery systems. These techniques have advantages and disadvantages with respect to each other considering dynamic range, selectivity, automation, compatibility with in vitro release media and cost per sample. Results: It is very important to determine the correct kinetic profile of active pharmaceutical substances. At this point, the analytical method used for in vitro release tests has become a very critical parameter to correctly assess the profiles. In this review, we provided an overview of analytical methods applied to the in vitro release assay of various nanopharmaceuticals. Conclusion: This review presents practical direction on analytical method selection for in vitro release test on nanopharmaceuticals. Moreover, precautions on analytical method selection, optimization and validation were discussed.
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- 2019
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23. Effective topical delivery systems for corticosteroids: dermatological and histological evaluations
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Eroğlu, İpek, primary, Azizoğlu, Erkan, additional, Özyazıcı, Mine, additional, Nenni, Merve, additional, Gürer Orhan, Hande, additional, Özbal, Seda, additional, Tekmen, Işıl, additional, Ertam, İlgen, additional, Ünal, İdil, additional, and Özer, Özgen, additional
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- 2014
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24. Effective topical delivery systems for corticosteroids: dermatological and histological evaluations.
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Eroğlu, İpek, Azizoğlu, Erkan, Özyazıcı, Mine, Nenni, Merve, Gürer Orhan, Hande, Özbal, Seda, Tekmen, Işıl, Ertam, İlgen, Ünal, İdil, and Özer, Özgen
- Subjects
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ATOPIC dermatitis treatment , *PHYSIOLOGICAL effects of adrenocortical hormones , *ATROPHY , *LIPOSOMES , *THERAPEUTIC use of nanostructured materials , *DISEASE risk factors , *THERAPEUTICS - Abstract
Atopic dermatitis (AD) is a chronic and relapsing skin disease with severe eczematous lesions. Long-term topical corticosteroid treatment can induce skin atrophy, hypopigmentation and transepidermal water loss (TEWL) increase. A new treatment approach was needed to reduce the risk by dermal targeting. For this purpose, Betamethasone valerate (BMV)/Diflucortolone valerate (DFV)-loaded liposomes (220–350 nm) were prepared and incorporated into chitosan gel to obtain adequate viscosity (∼13 000 cps). Drugs were localized in stratum corneum + epidermis of rat skin inex-vivopermeation studies. The toxicity was assessed on human fibroblast cells. In point ofin-vivostudies, pharmacodynamic responses, treatment efficacy and skin irritation were evaluated and compared with previously prepared nanoparticles. Liposome/nanoparticle in gel formulations produced higher paw edema inhibition in rats with respect to the commercial cream. Similar skin blanching effect with commercial creams was obtained via liposome in gels although they contain 10 times less drug. Dermatological scoring results, prognostic histological parameters and suppression of mast cell numbers showed higher treatment efficiency of liposome/nanoparticle in gel formulations in AD-induced rats. TEWL and erythema measurements confirmed these results. Overview of obtained results showed that liposomes might be an effective and safe carrier for corticosteroids in skin disease treatment. [ABSTRACT FROM PUBLISHER]
- Published
- 2016
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25. L-KARNİTİN YÜKLÜ POLİMERİK VE LİPİT BAZLI NANOPARTİKÜLER SİSTEMLERİN HAZIRLANMASI VE İN-VİTRO DEĞERLENDİRİLMESİ
- Author
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Yaşacan, Merve, Eroğlu, İpek, and Nanoteknoloji ve Nanotıp
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L-karnitin, Nanolipozom, PLGA nanopartikül, Omik Teknolojileri, Metabolomik ,L-carnitine, Nanoliposome, PLGA nanoparticles, Omics technology, Metabolomics - Abstract
L-carnitine has attracted attention in the treatment of important problems of era such as obesity, diabetes and regional slimming in recent years due to its metabolic effects that increase fat loss and muscle building in metabolism. L-carnitine, whose main function is the transfer of long chain fatty acids to mitochondria for beta oxidation, is supplied to the body from two sources, both exogenous and endogenous. The most important sources of L-carnitine taken from food are red meat and dairy products. The increase in artificial feeding and mass production in the food sector causes the amount of L-carnitine taken into the body to be insufficient therefore obesity rates are increasing. For that reason, L-carnitine is often taken as a supplement by athletes and people who want to lose weight. However, based on its low bioavailability (14-18%), short half-life (30-60 minutes) and inability to be stored in the body L-carnitine, frequent dosing is required for its effective treatment. In addition, L-canitine has defined impurities in the European and American Pharmacopoeia Monographs. Since frequent and high dose administration of L-carnitine known to have impurities, may increase the toxic and side effects so the development of its controlled release systems is become a very important issue. The future of nanoparticular drug delivery systems as controlled release systems depend on metabolic level evaluation of the effects on cell as well as clarification of nanoparticle structure and targeting mechanism. Nanoliposomes which have lipid bilayers similar to cell membranes, have the ability to increase the penetration of the active substance. Due to their increased stability, controlled release properties, polymeric nanoparticles are the most commonly used efficient and reliable systems among the nanocarrier systems. For this purpose, L-carnitine loaded nanoliposome and PLGA nanoparticle formulations have prepared, formulations characterization (particle size (PS), polydispersity Index (PDI), zeta potential (ZP), morphology (SEM), percentage of encapsulation efficiency (EE%), molecular structure/thermal properties clarification (FTIR/ATR, TGA)) and stability studies have made within the scope of the thesis. For L-carnitine encapsulated nanoliposome (Lipo-carnitine) and L-carnitine encapsulated PLGA nanoparticle (Nano-carnitine) formulations; PS values are 97.88±2.96 nm and 250.90±6.15 nm; PDI values are 0.35±0.01 and 0.22±0.03; ZP values are 6.36±0.54 mV and -32.80±2.26 mV; EE% values are 14.26±3.52 and 21.93±4.17, respectively. Comparative in-vitro release studies of Lipo-carnitine, Nano-carnitine formulations and free solution of L-carnitine at the same concentration have performed by dialysis membrane method. While control solution (free L-carnitine) have terminated 90% of the concentration at the end of the 1st hour with immediate release; Lipo-carnitine and Nano-carnitine formulations have showed a delayed controlled release profile which has obtained from 2, 4, 6, 8 and 12 hours release points, after the immediate release effect observed at the end of the 1st hour (59.90% and 65.19%, respectively). Kinetic models (zero, first, Higuchi, Hixson Crowell) have applied to the release profiles, determination coefficients (r2) were found 0.8539 and 0.9167 respectively, and both formulations have found to be compatible with the “First Order” kinetic model. Following release studies, the effectiveness of formulations have evaluated by metabolomic studies and pathway analyzes on cardiac fibroblast cells in-vitro conditions. The number of mitochondria in cardiac fibroblasts is high due to the energy demand. Thus, L-carnitine plays a critical role in the metabolism of these cells, which derive most of their energy from fatty acid oxidation. Metabolites which are the components of intracellular metabolism, have dedected and their levels have quantified by snaphots in metabolomic studies. By comparing the results obtained from metabolomic studies, the effects of free L-carnitine and L-carnitine loaded nano-systems on amino acid, carbohydrate and lipid metabolisms have evaluated. In parallel with prolonged release of L-carnitine, the Lipo-carnitine and Nano-carnitine formulations have found to be effective on amino acid, carbohydrate and lipid metabolisms. Nano-carnitine has found to be the most effective formulation for increasing amino acid levels with polar side chains. Nano-carnitine formulation has found to be more effective compared to control and Lipo-carnitine groups, at citric acid, aconitic acid, succinic acid, fumaric acid, malic acid, oxaloacetic acid levels which are belong to tricarboxylic acid cycle (TCA). When a TCA component called α-ketoglutarate is present in the sufficient amount, it turns into glutamate and acts on the metabolic pathways of ornithine, arginine and proline aminoacids. For the level of α-ketoglutarate, a significant (p
- Published
- 2019
26. İntratümöral Uygulamaya Yönelik Nanolipozom Formülasyonlarının Geliştirilmesi
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Yaman, Ümran, Eroğlu, İpek, Nanoteknoloji ve Nanotıp, and Nanobilim ve Nanoteknoloji Anabilim Dalı
- Subjects
Nanolipozom ,Poli-L-lizin ,Konu Başlıkları Listesi::Tıp::Eczacılık bilimi ,Pharmacy and Pharmacology ,Malign melanom ,5-Florourasil ,Eczacılık ve Farmakoloji ,İntratümöral enjeksiyon - Abstract
Purpose of our thesis; it has been aimed to prepare injectable formulations of the chemotherapeutic agent, which is effective in cancer cells, into nanoliposomes and to develop an intratumorally effective treatment approach. This will ensure that both chemotherapeutic drugs are effective at lower doses, as well as the associated side effects and resistance to growth. The targets of our policy are; 1. The increase of treatment efficiency by penetration with chemotherapeutic agent loaded nanoliposome formulations 2. Intratumoral injection of nanoliposomes to ensure that the drug only acts in the desired region 3. Reduction of drug side effects 4. Providing a faster and more effective response in advanced and drug-resistant tumors 5 Prolongation of patient survival by avoiding the spread of cancer In the event that our studies are successful, an innovative formulation will be developed as an alternative to the conventional preparations currently being used in therapy. Thus, a lower dose, shorter time and more effective treatment option for patients will be offered. TÜBİTAK Tezimizin amacı; kanser hücrelerinde sitotoksik etkili kemoterapötik ajanın nanolipozomlar içine yüklenerek enjekte edilebilir formülasyonlarının hazırlanması ve intratümöral olarak etkin bir tedavi yaklaşımı geliştirilmesi hedeflenmiştir. Böylece hem kemoterapötik ilaçların daha düşük dozlarda etkili olması sağlanacak hem de ilaca bağlı yan etkiler ve ilaca karşı gelişen direnç önlenebilecektir. Tezimizin hedefleri ise; 1. Kemoterapötik ajan yüklü nanolipozom formülasyonları ile penetrasyonun sağlanarak tedavi etkinliğinin arttırılması 2. Nanolipozomların intratümöral enjeksiyonu ile, ilacın sadece istenen bölgede etki göstermesinin sağlanması 3. İlaca bağlı yan etkilerin azaltılması 4. İleri düzey ve ilaç dirençli tümörlerde daha hızlı ve etkin bir yanıt sağlanması 5. Böylece kanserin yayılmasının önlenerek hastaların hayatta kalış sürelerinin uzatılması Çalışmalarımızın başarıyla sonuçlanması halinde, şu an tedavide kullanılmakta olan konvansiyonel preparatlara alternatif olarak yenilikçi bir formülasyon geliştirilmiş olacaktır. Böylece hastalar için daha düşük dozda, daha kısa sürede ve daha etkili bir tedavi seçeneği sunulacaktır.
- Published
- 2019
27. Antienflamatuvar etkili propolis yüklü lipozomal formülasyonların hazırlanması ve karakterizasyonu
- Author
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Aytekin, Ayşegül Aylin, Özer, Kevser Özgen, Eroğlu, İpek, Farmasötik Teknoloji Anabilim Dalı, Özer, Özgen, and Sağlık Bilimleri Enstitüsü
- Subjects
Gel ,Formularies ,Lipozom ,Antimikrobiyal/Antifungal Aktivite ,Jel Antioksidan Aktivite ,Antioxidant Activity ,Anti inflammatory agents ,Propolis ,Antioxidants ,Liposome ,Pharmacy and Pharmacology ,Liposomes ,Antimicrobial/Antifungal Activity ,Eczacılık ve Farmakoloji ,Antifungal agents - Abstract
Yara enfeksiyonları tüm dünyada milyonlarca insanı etkilemektedir. Özellikle kronik yaralarda uzun süren tedavi sürecinde gelişen enfeksiyonu önlemek amacıyla, antimikrobiyal ve antifungal etkiye sahip etkin maddeler kullanılmaktadır. Ayrıca, yara bölgesinde oluşan doku hasarının onarım sürecinde oksijen radikalleri oluşmaktadır. Antioksidan maddeler, yara bölgesini serbest radikal hasarlarına karşı korurlar ve böylece yara iyileşmesini hızlandırırlar. Doğal bir arı ürünü olan propolis, içeriği nedeniyle hem antimikrobiyal/antifungal hem de antioksidan özelliğe sahiptir. Bu nedenle, yara tedavisi için topikal formülasyonlar tasarlamak amacıyla kullanılmıştır. Konvansiyonel yaklaşımlara kıyasla daha etkili ve daha az yan etkili olması bakımından, nanotaşıyıcı sistemler, terapötik maddelerin uygulanması için önemli potansiyel uygulamalara sahiptir. Bu sistemler arasında lipozomlar, deri lipitlerine benzer yapıda olması nedeniyle etkin madde geçişini arttıran, veziküler yapıda ilaç taşıyıcı sistemler olarak dikkat çekmektedirler. Bu amaçla tez çalışmamızda ilk olarak propolis yüklü lipozom formülasyonları hazırlanmış, karakterizasyon (partikül boyutu dağılımı, polidispersite indeksi, zeta potansiyel, morfoloji, pH, yükleme kapasitesi, enkapsülasyon etkinliği) ve stabilite çalışmaları yapılmıştır. İn-vitro salım çalışmalarının ardından, optimize edilen formülasyonlar in-vitro antioksidan aktivite (serbest radikal süpürme kapasitesi ve troloks eşdeğer antioksidan kapasite tayini yöntemi) ve antimikrobiyal/antifungal aktivite çalışmaları ile değerlendirilmiştir. İdeal lipozom formülasyonu belirlenmiş ve topikal uygulamaya elverişli viskoziteyi sağlamak için, hidroksipropilmetilselüloz (HPMC) jel içinde dağıtılmıştır. Hazırlanan lipozomal jel formülasyonları; pH, reoloji, mekanik özellikler ve stabilite açısından değerlendirilmiştir. Sonuç olarak; bu tez çalışmasında hem antioksidan hem de antimikrobiyal etkiye sahip propolis yüklü lipozomal jel formülasyonları ile, yara tedavisinde topikal uygulamaya yönelik etkin ve yenilikçi bir alternatif geliştirilmiştir., Wound infections affect millions of people all over the world. Especially to avoid infections which develop during long treatment process of chronical wounds, active substances which have antimicrobial and antifungal effects are used. Additionally, oxygen radicals are generated during the healing process of tissue injuries in the wound area. Antioxidant substances protect the wound area against the effect of free radicals and accelerate the healing process. Propolis, a natural bee product, has both antimicrobial /antifungal and antioxidant characteristics because of its content. Therefore, propolis is used to develop topical formulations for wound treatment. As nanocarrier systems are more effective and have less side effects compare to conventional approach, they have important potential applications to implement therapeutic substances. Among these systems, liposomes attract notice as substances that enhance active substance transition because of having a similar structure to skin lipids, and as carrier systems which have vesicular structure. For this purpose, in our thesis study, initially propolis loaded liposome formulations were prepared and characterization (particle size distribution, polidispersity index, zeta potential, morfology, pH, loading capacity, encapsulation efficiency) and stability studies were performed. After the in-vitro studies, formulations that are optimized have been evaluated with in-vitro antioxidant activity (free radical scavenging capacity and trolox equivalent antioxidant capacity) and antimicrobial/antifungal activity studies. Ideal liposome formulation was defined and it was distributed in hidroksipropilmetilselüloz (HPMC) gel in order to make suitable viscosity for topical application. The prepared liposomal gel formulations were evaluated in terms of pH, rheology, mechanic chracteristics and stability. In conclusion; in this thesis study, an effective and innovator alternative was developed for topical application in wound treatment with propolis loaded liposomal gel formulations which have both antioxidant and antimicrobial effects.
- Published
- 2018
28. Hyaluronan mikropartikülleri içeren dermal sistemlerin doku onarımı üzerine etkilerinin incelenmesi
- Author
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Simsolo, Erol Eli, Özer, Kevser Özgen, Eroğlu, İpek, and Farmasötik Teknoloji Anabilim Dalı
- Subjects
Skin diseases ,Pharmacy and Pharmacology ,Hyaluronic acid ,Caffeine ,Phosphatidylcholines ,Cellulitis ,Dermatology ,Drug carriers ,Eczacılık ve Farmakoloji ,Microparticles ,Dermatoloji ,Delayed action preparations - Abstract
Selülit, yağ hücrelerinin irileşmesi ve derinin esnekliğinin azalması sonucu olarak dermal hasara neden olan kozmetik bir bozukluktur. Bitkisel ekstrelerden elde edilen kafein, kan dolaşımını arttırıcı, lipolitik ve lipojenezi inhibe edici etkileri nedeniyle selülit tedavisinde tercih edilen bir maddedir. Mikropartiküller farmasötik teknoloji alanında sıklıkla hazırlanan ilaç taşıyıcı sistemler olup, polimerik yapıları nedeniyle etkin maddenin kontrollü ve uzatılmış salımını sağlarlar. Bu çalışmada, selülit tedavisinde uzun süre etkili yeni bir bir formülasyon geliştirilmesi ve etkinliklerinin optimize edilmesi amaçlandı.Bu amaçla, yeni hücre oluşumu/hareketliliğinin devamını sağlayan ve nemlendirme özelliğine sahip doğal deri bileşenlerinden olan hyalüronik asit (hyaluronan-HA) ile kafein yüklü mikropartiküller püskürterek kurutma yöntemi ile hazırlandı. Hazırlanan mikropartikül formülasyonları topikal uygulamada uygun viskoziteyi verecek lesitin organojel formülasyonları içerisinde dağıtıldı. Hazırlanan organojel formülasyonları pH, viskozite, reolojik ve mekanik özellikler ile karakterize edilirken, mikropartikül formülasyonları partikül büyüklüğü, morfolojik incelemeler ve etkin madde yükleme etkinliği parametreleri ile karakterize edildi. Formülasyonların stabilite çalışmaları sonucunda 25 ± 2ºC ve % 60 ± 5 relatif rutubet koşullarında 6 ay süre ile stabil kaldıkları belirlendi. Termal analizlerin ardından, mikropartiküllerin diyaliz membrandan in vitro salım çalışmaları gerçekleştirildi. UV altında sterilize edildikten sonra, sitotoksik etkileri hücre kültürü çalışmaları ile değerlendirildi. Formülasyonların sıçan karın derisinden ex-vivo difüzyon çalışmaları yapıldı. Mikropartikül içeren organojel formülasyonlarından kafein salımının anlamlı derecede uzadığı belirlendi (p
- Published
- 2013
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