37 results on '"Ertas, Busra"'
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2. A novel high-efficiency transdermal patches for combinational therapy of Alzheimer's disease: Donepezil/vitamin B12-loaded nanofibers
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Ertas, Busra, Onay, Irem Nur, Yilmaz-Goler, Ayse Mine, Karademir-Yilmaz, Betul, Aslan, Ismail, and Cam, Muhammet Emin
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- 2023
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3. Rosa canina L. improves learning and memory-associated cognitive impairment by regulating glucose levels and reducing hippocampal insulin resistance in high-fat diet/streptozotocin-induced diabetic rats
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Ertas, Busra, Hazar-Yavuz, Ayse Nur, Topal, Fadime, Keles-Kaya, Rumeysa, Karakus, Özge, Ozcan, Gul Sinemcan, Taskin, Turgut, and Cam, Muhammet Emin
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- 2023
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4. In vitro and in vivo evaluation of multi-target-directed Rivastigmine/Memantine/Gingko biloba-loaded nanofibers against Alzheimer's disease
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Veysanoglu, Servan, Ertas, Busra, Guler, Ece, Topal, Fadime, Ozcan, Gul Sinemcan, Duruksu, Gokhan, Ece, Burak, Cam, Cansun Sahin, Aydemir, Oguzhan, and Cam, Muhammet Emin
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- 2023
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5. Histological and biochemical effects of an ethanolic extract of Myrtus communis leaf on the pancreases of rats fed high fat diets
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Kabatas, Gul Sinemcan, primary, Ertas, Busra, additional, Sen, Ali, additional, Sener, Goksel, additional, Ercan, Feriha, additional, and Akakin, Dilek, additional
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- 2024
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6. Synergistic anticancer effects of metformin and Achillea vermicularis Trin-loaded nanofibers on human pancreatic cancer cell line: An in vitro study
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Babazade, Ravana, Beyzanur Polat, Elif, Ertas, Busra, Ozcan, Gul Sinemcan, Kiyak Kirmaci, Humeysa, Tatar, Esra, Taskin, Turgut, Yazir, Yusufhan, and Emin Cam, Muhammet
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- 2022
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7. A novel multi-target strategy for Alzheimer's disease treatment via sublingual route: Donepezil/memantine/curcumin-loaded nanofibers
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Topal, Fadime, Ertas, Busra, Guler, Ece, Gurbuz, Fatmanur, Ozcan, Gul Sinemcan, Aydemir, Oguzhan, Bocekci, Veysel Gokhan, Duruksu, Gokhan, Sahin Cam, Cansun, Yazir, Yusufhan, Gunduz, Oguzhan, and Cam, Muhammet Emin
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- 2022
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8. Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study
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Cam, Muhammet Emin, Ertas, Busra, Alenezi, Hussain, Hazar-Yavuz, Ayse Nur, Cesur, Sumeyye, Ozcan, Gul Sinemcan, Ekentok, Ceyda, Guler, Ece, Katsakouli, Christina, Demirbas, Zehra, Akakin, Dilek, Eroglu, Mehmet Sayip, Kabasakal, Levent, Gunduz, Oguzhan, and Edirisinghe, Mohan
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- 2021
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9. Histological and biochemical effects of an ethanolic extract of <italic>Myrtus communis</italic> leaf on the pancreases of rats fed high fat diets.
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Kabatas, Gul Sinemcan, Ertas, Busra, Sen, Ali, Sener, Goksel, Ercan, Feriha, and Akakin, Dilek
- Abstract
We investigated the effects of an ethanolic extract of
Myrtus communis subsp.communis (MC) leaves on the pancreases of rats fed with a high fat diet (HFD). Wistar albino rats were fed either with standard lab chow (Control group) or with a 45% fat diet (HFD and HFD+MC groups) for 4 months, with the MC extract (100 mg/kg) being administered by orogastric gavage to rats in the HFD+MC group during the last month. Blood and pancreas samples were collected from all experimental groups at the end of the study. Insulin and leptin levels, and the lipid profile, were analyzed in the blood serum. Pancreatic injury was assessed histologically. Insulin, nuclear factor kappa beta (NF-κB), and alpha-smooth muscle actin (α-SMA) were assessed using immunohistochemistry. Apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) immunohistochemistry. In addition, oxidant/antioxidant activity was analyzed by biochemical methods. Increased body weight, serum insulin and leptin levels, blood glucose level and pancreatic tissue malondialdehyde (MDA), 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels, myeloperoxidase (MPO) activity and decreased tissue glutathione (GSH) level were observed in the HFD group compared to the Control group, in addition to dyslipidemia. An increased histopathological damage score, pancreatic islet area, insulin, TUNEL, NF-κB and α-SMA immunoreactivity were seen in animals from the HFD group compared to the Control group. However, such pathological changes were reduced in the HFD+MC group. Our data indicate further investigation of MC extract as a therapeutic adjuvant for HFD-induced pancreatic injury, acting via anti-inflammatory and antioxidant mechanisms, is worth carrying out. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. Apocynin exhibits an ameliorative effect on endothelial dysfunction/ atherosclerosis-related factors in high-fat diet-induced obesity in rats.
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BULBUL AYCİ, Nurdan, ERTAS, Busra, KELEŞ KAYA, Rumeysa, KOCYIGIT SEVİNÇ, Sevgi, AMURAN, Gokce Gullu, ERCAN, Feriha, ŞENER, Göksel, ORUN, Oya, AKKIPRIK, Mustafa, and CETINEL, Sule
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BIOLOGICAL models , *HIGH density lipoproteins , *NUCLEOSIDES , *LEPTIN , *RESEARCH funding , *APOPTOSIS , *ATHEROSCLEROSIS , *ENDOTHELIUM , *DIETARY fats , *INSULIN , *OXIDATIVE stress , *RATS , *LOW density lipoproteins , *OXIDOREDUCTASES , *ANIMAL experimentation , *CHOLESTEROL , *GLYCOSIDES , *TRIGLYCERIDES , *OBESITY , *CELLS , *THORACIC aorta - Abstract
Objective: The aim of this study was to reveal the effect of apocynin (APO) on the factors involved in obesity-related endothelial dysfunction (ED) and atherosclerosis (AS). Materials and Methods: Male Wistar albino rats were divided into control (CNT), high-fat diet (HFD) and HFD+APO groups. HFD and HFD+APO groups were fed HFD for sixteen weeks. APO (25 mg/kg) was administered to the HFD+APO group for the last four weeks. The effects of APO on: AS-related metabolic parameters (triglyceride, total cholesterol, high-density lipoprotein-cholesterol, insulin and leptin), oxidative stress (OS), [ malondialdehyde, glutathione, nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase-2, oxidised-low-density lipoprotein (ox-LDL) and 8-hydroxy-2-deoxyguanosine], low-density lipoprotein and ox-LDL uptake potential (activin receptor-like kinase-1 and lectin-like oxidized low-density lipoprotein receptor-1, respectively), tissue inflammation (myeloperoxidase, monocyte-chemoattractant-protein-1, tumor necrosis factor-alpha), ED (endothelial-nitric oxide synthase, inducible-nitric oxide synthase, nitric oxide), programmed cell death (terminal deoxynucleotidyl-transferase-dUTP-nick-end labeling, cleaved-poly-ADP-ribose-polymerase, gasdermin-D N-terminal fragment, caspase-1), smooth muscle cell transformation (alpha-smooth muscle actin), histology and ultrastructure of thoracic aorta were evaluated. Results: In obesity, APO had an ameliorative effect on metabolic parameters, OS, inflammation, ED, programmed cell death and ox-LDL uptake potential, but not on foam cell formation and LDL uptake potential. Conclusion: Apocynin may improve ED and AS in obesity by suppressing OS-linked factors involved in the early stage of AS. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Ameliorative effects of Myrtus communis L. extract involving the inhibition of oxidative stress on high fat diet-induced testis damage in rats.
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Coskunlu, Busra, Koroglu, M. Kutay, Hersek, Irem, Ertas, Busra, Sen, Ali, Sener, Goksel, and Ercan, Feriha
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SPERMATOGENESIS ,OXIDATIVE stress ,SPERMATOZOA ,HIGH-fat diet ,MYRTLE (Plants) ,TESTIS ,HIGH density lipoproteins ,SEMINIFEROUS tubules - Abstract
The possible protective effects of Myrtus communis L. (MC) extract on a high fat diet (HFD)-induced testicular injury in a rat model were investigated using histological and biochemical methods. Wistar albino rats were divided into three groups: a standard diet control group; a HFD group; and an HFD+MC group. The HFD and HFD+MC groups were fed with a HFD for 16 weeks. MC extract (100 mg/kg) was given orally five days a week to the rats in the HFD+MC group during the last four weeks of the experiment. Leptin, triglyceride, high-density lipoproteins, cholesterol, estrogen, testosterone, LH and FSH were analyzed in blood serum. Sperm parameters were evaluated from the epididymis. Testicular morphology, proliferative, apoptotic and NADPH oxidase-2 (NOX2)-positive cells were evaluated histologically. Testicular oxidative stress parameters were analyzed biochemically. In the HFD group, lipid and hormone profiles were changed, abnormal spermatozoa, degenerated seminiferous tubules with apoptotic and NOX2-positive cells were increased in number, and sperm motility and germinal proliferative cells decreased compared to the control group. Moreover, testicular malondialdehyde, 8-hydroxy-2-deoxyguanosine and myeloperoxidase levels increased, whereas glutathione and superoxide dismutase levels decreased in the HFD group compared to the control group. All these histological and biochemical features were ameliorated by MC treatment of HFD-fed rats. In conclusion, HFD caused alterations in sperm parameters and testicular morphology by increasing oxidative damage and apoptosis. MC extract may have potential protective effects by inhibiting oxidative damage. [ABSTRACT FROM AUTHOR]
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- 2024
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12. The comparision of glybenclamide and metformin-loaded bacterial cellulose/gelatin nanofibres produced by a portable electrohydrodynamic gun for diabetic wound healing
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Cam, Muhammet Emin, Crabbe-Mann, Maryam, Alenezi, Hussain, Hazar-Yavuz, Ayse Nur, Ertas, Busra, Ekentok, Ceyda, Ozcan, Gul Sinemcan, Topal, Fadime, Guler, Ece, Yazir, Yusufhan, Parhizkar, Maryam, and Edirisinghe, Mohan
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- 2020
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13. The effect of Myrtus communis L. extract on nephrolithiasis model in rats.
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Ertas, Busra, Dorucu, Dogancan, Gulerturk, Oznur, Sen, Ali, Cevik, Ozge, Cetinel, Sule, Eker, Pinar, Akgun, Asuman, Sener, Tarik Emre, and Sener, Goksel
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KIDNEY stones ,RATS ,OXIDATIVE stress ,KIDNEY failure ,HISTOPATHOLOGY - Abstract
OBJECTIVE: Nephrolithiasis is a common urological disease that can lead to renal failure. Oxidative stress has been shown to be a contributing factor for nephrolithiasis and many agents have been studied to prevent and treat oxidative stress-related nephrolithiasis and renal damage. Myrtus communis (MC) extract has been shown to be an important antioxidant in different animal models. In this study, MC extract was administered preventively or therapeutically to rats with kidney stones, and its effectiveness was investigated. METHODS: Wistar albino rats were divided into four groups (n=8); control (C), ethylene glycol (EG), EG+preventive MC, and EG+curative MC groups. The nephrolithiasis model was created by adding 0.75% EG to drinking water for 8 weeks. Ultimately, 24-hour urine was collected to measure calcium, citrate, and creatinine levels. After decapitation, kidney tissues were harvested for histological analyses, measurement of osteopontin and 8-hydroxydeoxyguanosine (8-OHdG) levels, and N-acetyl-β-glucosaminidase (NAG), myeloperoxidase (MPO) and caspase-3 activities. RESULTS: In 24-hour urine samples, calcium, citrate and creatinine levels were decreased in the EG group, while oxalate levels were increased and in treatment groups these parameters returned to control levels. MPO, 8-OHdG, caspase-3 and NAG activity were significantly increased in tissue and these changes were reversed in both MC groups. Histological findings also supported the biochemical parameters. CONCLUSION: MC can reduce oxidative stress and histopathological changes in kidney tissues in rat nephrolithiasis model when used as either a preventive or therapeutic agent. If supported with further clinical trials, MC might have clinical implications in preventing oxidative renal cell injury and ultimately kidney stone formation. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Ethanolic extract of cotinus coggygria leaves attenuates crystalluria and kidney damage in ethylene glycol-induced urolithiasis in rats.
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Gumru, Salih, Ozgur, Gunal, Ertas, Busra, Sen, Ali, Eker, Pinar, and Sener, Tarik Emre
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ETHANOL ,RHUS cotinus ,KIDNEY disease treatments ,URINARY calculi ,OXIDATIVE stress - Abstract
OBJECTIVE: Nephrolithiasis is a common cause of kidney insufficiency. Nephrolithiasis is proven to be the result of various biochemical and inflammatory processes that result in crystal formation and subsequent aggregation. Cotinus coggygria L. (CCog) is a plant extract which has been used as a Turkish remedy for kidney stones. With this study, we planned to evaluate the effects of CCog extract in ethylene glycol (EG)-induced nephrolithiasis model in rats. METHODS: The study group comprised 32 Wistar albino rats which were divided into Control (C), EG, CCog Prophylaxis (CC+EG+CC), and CCog Treatment (EG+CC) groups. Stone formation was induced by adding EG (0.75%) into rat's drinking water. Normal drinking water was given to Control group for 8 weeks. Throughout the study period of 8 weeks, EG group was given only EG (0.75%) and CC+EG+CC group was given both EG and CCog. In EG+CC group, EG (0.75%) was given for 8 weeks whereas CCog was given for the past 4 weeks. After the 8th week, 24-h urine samples were collected. Rats were then sacrificed and kidney tissue samples were harvested. RESULTS: Metabolites (calcium, citrate) and creatinine in 24 h urine samples were decreased in CC+EG+CC and EG+CC groups. While hyperoxaluria was observed in the EG group, oxalate levels were similar to control levels in the P-CCog and C-CCog groups. The N-acetyl-ß-glucosaminidase and myeloperoxidase activities were both increased in EG group and these parameters were significantly decreased on CCog treatment. CONCLUSION: We can conclude that C. coggygria extract can have beneficial effect on lowering concentration of stone-forming metabolites in urine and consequently protect renal tissues from damage due to nephrolithiasis. C. coggygria extract can be considered as a potential prophylactic and therapeutic option in high-risk stone formers. Furthermore, our data confirm ethnobotanical use of CC against nephrolithiasis. [ABSTRACT FROM AUTHOR]
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- 2023
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15. In Vitro and in Vivo Evaluation of Multi-Target-Directed Rivastigmine/Memantine/Gingko Biloba-Loaded Nanofibers Against Alzheimer's Disease
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Veysanoglu, Servan, primary, Ertas, Busra, additional, Guler, Ece, additional, Topal, Fadime, additional, Ozcan, Gul Sinemcan, additional, Duruksu, Gökhan, additional, Ece, Burak, additional, Cam, Cansun Sahin, additional, Aydemir, Oguzhan, additional, and CAM, Muhammet Emin, additional
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- 2023
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16. Rosa Canina L. Improves Learning and Memory-Associated Cognitive Impairment by Regulating Glucose Levels and Reducing Hippocampal Insulin Resistance in High-Fat Diet/Streptozotocin-Induced Diabetic Rats
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Ertas, Busra, primary, Hazar-Yavuz, Ayse Nur, additional, Topal, Fadime, additional, Keles-Kaya, Rumeysa, additional, Karakus, Ozge, additional, Ozcan, Gul Sinemcan, additional, Yazır, Yusufhan, additional, Taskin, Turgut, additional, and CAM, Muhammet Emin, additional
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- 2023
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17. Carvacrol Improves Cognitive Dysfunction by Decreasing Amyloid-ß Accumulation and Regulating Neuroinflammation in Ovariectomized Renovascular Hypertensive Rats
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Bayraktar, Duygu, primary, Ertas, Busra, additional, Aydın, Yasemin, additional, and Sener, Goksel, additional
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- 2023
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18. Myrtus communis L. Extract Ameliorates High Fat Diet Induced Kidney and Bladder Damage by Inhibiting Oxidative Stress and Inflammation.
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Mustafaoglu, Fatma Kanpalta, Ertas, Busra, Sen, Ali, Akakin, Dilek, Sener, Goksel, and Ercan, Feriha
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MYRTLE (Plants) , *HIGH-fat diet , *URINARY organs , *CONTROL groups , *MALONDIALDEHYDE - Abstract
Objective: Obesity is associated with many diseases, including urinary system disorders such as chronic kidney disease and overactive bladder syndrome. Myrtus communis L. (MC) extract has been reported to have antioxidant and anti-inflammatory effects. The aim of this study was to investigate the protective effects of MC extract on high-fat diet (HFD)-induced kidney and bladder damage. Materials and Methods: Wistar albino male rats were divided into three experimental groups: control, HFD and HFD+MC. Experimental groups were fed a standard diet (control group) or HFD (HFD and HFD+MC groups) for 16 weeks. MC extract (100 mg/kg) was administered to the HFD+MC group orally during the last 4 weeks (5 days/week) of the experiment. Highdensity lipoprotein, total cholesterol, triglyceride and leptin levels were measured in blood serum. Tissue malondialdehyde (MDA), glutathione (GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG) and myeloperoxidase (MPO) levels were evaluated biochemically. Kidney and bladder morphology, NADPH oxidase-2 (NOX-2) and nuclear factor-kappa B (NF-KB)-positive and apoptotic cells were evaluated histologically. Results: Lipid profiles altered and leptin levels increased in blood serum. MDA, 8-OHdG and MPO levels increased and GSH level decreased in kidney and bladder in the HFD group. Moreover, degenerated kidney and bladder morphology, increased NOX-2 and NF-κB-positive and apoptotic cells were observed in this group. All of these biochemical and histological parameters were ameliorated in the HFD+MC group. Conclusion: HFD-induced obesity causes kidney and bladder damage by oxidative and inflammatory processes. MC extract may reduce oxidative stress and inflammation and play a protective role in obesity-related kidney and bladder damage. [ABSTRACT FROM AUTHOR]
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- 2022
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19. Beta vulgaris var. cicla improves memory deficits in intracerebroventricular streptozotocin injected rats: Role on neuroinflammation
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ERTAS, Busra, primary, TOPAL, Fadime, additional, GULHAN, Rezzan, additional, YANARDAĞ, Refiye, additional, SAÇAN, Özlem, additional, and ŞENER, Göksel, additional
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- 2021
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20. Dose-dependent effect of Scolymus hispanicus L. (sevketibostan) on ethylene glycol-induced kidney stone disease in rats.
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Coşkun, N. Kamer, Coşkun, Ali, Ertas, Busra, Ahmad, Sarfraz, Özdöl, Mehmet Ümit, Çankaya, Soner, Çetinkol, Yeliz, Ozel, Yahya, and Elçioğlu, H Kübra
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ETHYLENE glycol ,KIDNEY stones ,CALCULI ,CALCIUM oxalate ,AMINOTRANSFERASES ,ALKALINE phosphatase - Abstract
Kidney stone, also known as calcium oxalate nephrolithiasis, is one of the most common diseases worldwide. Calculi usually forms when urine becomes supersaturated with particular calcium salts such as calcium oxalate. In the present study, we investigated the ameliorative potential of the root extract of the Common golden thistle, Scolymus hispanicus L. (SH) on rats with ethylene glycol (EG) induced kidney stone disease. Sprague-Dawley rats, each weighing 250-300 g, were divided into three groups (n=6 per group): (i) Control (C); (ii) EG; and (iii) EG+SH. To induce nephrolithiasis, the rats received 1% of EG with drinking water, while the C group received normal drinking water during the study. SH extract 2 g/kg was added to the treatment from the 4th week onwards in EG+SH group. At the end of each experiment, rats were decapacitated and serum levels of calcium, magnesium, phosphorus, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were assessed in all groups at 0, 4, and 8 weeks. Oxalic acid and creatininelevels were measured in urine samples collected at 24 h in metabolic cages. Renal tissues were evaluated histopathologically at the end of the experiment. After 8 weeks, serum creatinine levels were found decreased in the SH group while increased in the EG group. Serum magnesium and AST levels were also found decreased in the EG group, however, SH treatment reversed these values. The SH treatment also increased urinary oxalic acid levels. When the kidney tissue of EG group was examined, there was a high level of crystal/stone, especially in the renal cortex. In kidney tissues of the SH group, only small amounts of crystal/stone were observed. Our experimental findings have demonstrated the ameliorative potential of the aqueous extracts of S. hispanicus roots and shells on EG-induced in the kidney stones in rats. Isolation of active compounds of SH would be desirable to understand the biochemical mechanism behind the process better. [ABSTRACT FROM AUTHOR]
- Published
- 2022
21. Effect of myrtus communis extract on serum cytokines in angiotensin dependent hypertensive rats
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Yakut, Zatiye, primary, Ertas, Busra, additional, Sen, Ali, additional, and Sener, Goksel, additional
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- 2020
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22. Myrtus communisleaf extract protects against cerulein‐induced acute pancreatitis in rats
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Ozbeyli, Dilek, primary, Sen, Ali, additional, Cilingir Kaya, Ozlem Tugce, additional, Ertas, Busra, additional, Aydemir, Sezgin, additional, Ozkan, Naziye, additional, Yuksel, Meral, additional, and Sener, Goksel, additional
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- 2019
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23. Protective Effects of Petroselinum crispum (Parsley) Extract Against Methotrexate-Induced Hepatotoxicity.
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Ertas, Busra, Turan, Feyza Berin, Ozbeyli, Dilek, Yanardag, Refiye, Sacan, Ozlem, and Sener, Goksel
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PARSLEY , *HEPATOTOXICOLOGY , *METHOTREXATE , *SPRAGUE Dawley rats , *ANTIOXIDANTS - Abstract
Objective: By inhibiting the synthesis of thymidine and purine, and thereby DNA synthesis, Methotrexate (MTX), suppresses the proliferation of cancer cells. It is thought that the side-effect mechanism is related to oxidant molecules derived from MTX metabolism. In this study, we examined whether the Petroselinum crispum extracts (PCr; parsley) of which the antioxidant properties have been previously shown, was protective against MTX induced liver damage. Materials and Methods: Sprague Dawley rats (female/male; 200-250 g) were used. MTX was injected intraperitoneally and PCr extract was given orally. A single dose of 20mg/kg MTX was administered to the groups that were to experience hepatotoxicity. Then, a physiological saline (MTX group) or PCr (2 g/kg, MTX + PCr group) treatment was applied for 5 days. The same treatments were applied to the other groups (control group, PCr group) for 5 days after a single dose saline injection. At the end of the study, the biochemical parameters were examined in the blood and liver tissues taken from animals sacrificed by decapitation. Results: MTX caused a significant increase in malondialdehyde and collagen levels and myeloperoxidase and caspase-3 activities, while glutathione levels were found to have decreased. PCr treatment showed protective efficacy by preventing these increases. Conclusion: It appears that the administration of PCr to MTX treated rats prevented the accumulation of lipid peroxides, inflamatory reactions and depletion of antioxidant glutathione, and thus protected liver tissues against oxidative stress. [ABSTRACT FROM AUTHOR]
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- 2021
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24. The methanolic extract of Thymus praecox subsp. skorpilii var. skorpilii restores glucose homeostasis, ameliorates insulin resistance and improves pancreatic β-cell function on streptozotocin/nicotinamide-induced type 2 diabetic rats
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Cam, Muhammet Emin, primary, Hazar-Yavuz, Ayse Nur, additional, Yildiz, Sila, additional, Ertas, Busra, additional, Ayaz Adakul, Betul, additional, Taskin, Turgut, additional, Alan, Saadet, additional, and Kabasakal, Levent, additional
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- 2019
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25. The inhibitory effects of Salvia triloba and Thymus praecox on acetylcholinesterase activity in diabetic rats
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Ertas, Busra, Cam, Muhammet Emin, Adakul, Betul Ayaz, Sener, Goksel, and Kabasakal, Levent
- Abstract
Abstract Title: The inhibitory effects of Salvia triloba and Thymus praecox on acetylcholinesterase activity in diabetic ratsBackgroundDiabetes mellitus (DM) is a metabolic disease characterized by long-term complications including cerebrovascular injury and cognitive disorders. Acetylcholinesterase (AChE) which is involved in numerous pathways in the central nervous system regulates the level of acetylcholine (ACh) which plays vital functions such as memory, learning, modulation of cerebral blood flow, movement control, neurotransmission as well (1). Recent studies have shown that hypoinsulinemia and insulin resistance is the reason of the decreased level of ACh and contribute the possible biochemical link between DM and cognitive disorders including Alzheimeru2019s disease. Studies with plants have shed light on the correction of cognitive functions of diabetes mellitus with increased AChE activity. Some Salvia and Thymus species of the Lamiaceae family have antidiabetic effects (2). There is no study on antidiabetic activity of Salvia triloba and Thymus praecox.AimTo investigate the effects of Salvia triloba and Thymus praecox on brain acetylcholinesterase activities in diabetes mellitus. With the help of obtained results, further studies will be carried out for ameliorating cognitive dysfunction effectively and also supporting current treatments.MethodThe rats were divided into five groups (6 rats per group): Group-I: Normal control, Group-II: Diabetic control, Group-III: Treatment with Salvia triloba methanolic extract, (200 mg/kg/day, i.g.), Group-IV: Treatment with Thymus praecox methanolic extract, (100mg/kg/day, i.g.), Group-V: Treatment with Metformin (400 mg/kg/day, i.g.). Type 2 DM was induced experimentally in these groups of rats by the intraperitoneal administration of 55 mg/kg of STZ and 100 mg/kg nicotinamide, except control group. Treatments were carried out after induction of DM and lasted for 3 weeks except decapitation day. The AChE enzymatic assay was determined according to the method of Ellman et al. The reaction mixture (2 mL final volume) contained 100 mM K+-phosphate buffer, pH 7.5 and 1 mM 5,50-dithiobisnitrobenzoic acid (DTNB). The method is based on the formation of the yellow anion, 5, 50- dithio-bis-acid-nitrobenzoic, measured by absorbance at 412 nm during 2 min incubation at 25u00b0C. The enzyme (40u201350 mg of protein) was pre-incubated for 2 min. The reaction was initiated by adding 0.8 mM acetylthiocholine iodide (AcSCh). All samples were in triplicate readings and the enzyme activities were expressed in mmol AcSCh/h/mg of protein.ResultsThymus praecox and Salvia triloba in methanolic extract considerably decreased (pu02c20.0001) blood glucose levels compared to diabetic rats and performed this drop for 3 weeks. Thymus praecox extract reached its maximum hypoglycemic effect (141.83u00b17.31, pu02c20.0001) in 2 weeks. At the same time, Thymus praecox and Salvia triloba extract also showed strong hypoglycemic effect (164.83u00b113.30, pu02c20.0001). The diabetic group exhibited a significant increase (pu02c20.001) in AChE activity in prefrontal cortex compared to the control and the treatment groups. However Salvia triloba extract and Thymus praecox extract reverted this increase (pu02c20.001) in AChE activity induced by diabetes after 3 weeks of treatment. The metformin group decreased (%47, pu02c20.001) in AChE activity compared to diabetic groups. Salvia triloba extract (%37, pu02c20.001) and Thymus praecox extract group (%41, pu02c20.001) a considerably decrease in AChE activity compared to diabetic group. The metformin group did not show a significant difference in enzyme activity compared to the control group. However, Salvia triloba and Thymus praecox increased (pu02c20.05) in AChE activity compared to control groups.DiscussionOur study results showed that an increase in AChE activity in type 2 diabetic rat brains. This increase in AChE activity in the T2DM rat model can be linked to depletion in cholinergic actions leading to defective neurotransmission and cognitive dysfunction as a result of decreased levels of ACh in the synaptic cleft. We showed that the reestablishment of AChE activity by Salvia triloba extract and Thymus praecox extract which for the first time demonstrated antidiabetic activities can contribute to the maintenance of cholinergic signaling in diabetic rats. In the light of the results, these plants give a valuable direction for researchers on the future for diabetes-related cognitive disorders. 1. Jacobson AM., et al., Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications Study Research Group, Long-term effect of diabetes and its treatment on cognitive function. N Engl J Med. 2007;356(18):1842u20131852.2. Davids D., et al., Ethnobotanical survey of medicinal plants used to manage High Blood Pressure and Type 2 Diabetes Mellitus in Bitterfontein, Western Cape Province, South Africa, J Ethnopharmacol. 2016 Dec 24;194:755-766.
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- 2017
26. The methanolic extract of Thymus praecox subsp. skorpilii var. skorpilii restores glucose homeostasis and ameliorates insulin resistance on streptozotocin/nicotinamide-induced type 2 diabetic rats
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Cam, Muhammet Emin, primary, Yildiz, Sila, additional, Hazar-Yavuz, Ayse Nur, additional, Ertas, Busra, additional, Taskin, Turgut, additional, and Kabasakal, Levent, additional
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- 2018
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27. In vitroand in vivoevaluation of multi-target-directed Rivastigmine/Memantine/Gingko biloba-loaded nanofibers against Alzheimer's disease
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Veysanoglu, Servan, Ertas, Busra, Guler, Ece, Topal, Fadime, Ozcan, Gul Sinemcan, Duruksu, Gokhan, Ece, Burak, Cam, Cansun Sahin, Aydemir, Oguzhan, and Cam, Muhammet Emin
- Abstract
The highly complex pathophysiology of Alzheimer's disease (AD) and the low bioavailability of the currently used drugs make it necessary to offer versatile treatment options. In this study, polyvinyl alcohol (PVA)/polyvinylpyrrolidone (PVP) nanofibers (NFs) were loaded with rivastigmine (RIV), memantine (MM), and Gingko biloba(GB) extract, and their anti-Alzheimer's effects were investigated in vitroby cell culture and in vivoby animal experiments. The characterization studies, including FTIR, XRD, and DSC analysis, showed that the fibers exhibited a good compatibility. The rapid disintegration (in 5.2 s) and complete dissolution (in 120 s) of RIV/MM/GB-loaded NF indicated their suitability for sublingual application. Cell viability analysis revealed that RIV/MM/GB-loaded NFs can be considered as safe and promising candidates. RIV/MM/GB-loaded NFs tremendously improved the learning and memory impairment seen in the AD model created by the injection of intracerebroventricular-streptozotocin into rats. Moreover, RIV/MM/GB-loaded NF stimulated stem cells to improve neuronal differentiation properties and significantly decreased the level of β-amyloid, tau, APP, TNF-α, AChE, and GSK-3β in the brain. The histopathological and immunostaining assays demonstrated that RIV/MM/GB-loaded NF reduced β-amyloid formation and tau hyperphosphorylation in the hippocampus and cerebral cortex. Thus, a novel and promising treatment strategy could be developed that has several advantages, such as ease of sublingual administration, reduced frequency of administration associated with lower drug amounts, and finally reduced toxicity and cost.
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- 2023
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28. Myrtus communis leaf extract protects against cerulein‐induced acute pancreatitis in rats.
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Ozbeyli, Dilek, Sen, Ali, Cilingir Kaya, Ozlem Tugce, Ertas, Busra, Aydemir, Sezgin, Ozkan, Naziye, Yuksel, Meral, and Sener, Goksel
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MYRTLE (Plants) ,PANCREATITIS ,RATS ,THERAPEUTICS ,APTAMERS ,INTERLEUKIN-6 ,OXIDANT status ,GLUTATHIONE - Abstract
In this study, the aim was to examine the potential protective effects of Myrtus communis subsp. communis leaf ethanol extract (MC) treatment against acute pancreatitis (AP) in rats. Thirty‐two rats were grouped as the saline‐pretreated control (C), MC‐pretreated control (MC), saline‐pretreated AP (AP), and MC‐pretreated AP (MC + AP) groups. To induce AP, cerulein was administered (50 µg/kg) two times. The rats were given MC for 14 days before cerulein injection. Six hours after the final cerulein injection, the rats were sacrificed. Pancreatic damage was associated with an increase in the serum activity of lipase and amylase, the pancreatic activity of myeloperoxidase, and the pancreatic level of malondialdehyde, interleukin‐1β, and interleukin‐6. AP also led to a decrease in the pancreatic level of anti‐inflammatory interleukin‐10 and glutathione. Pretreatment with MC before the induction of AP significantly reduced the pancreatic damage observed during the histological examination as well as reversed the biochemical changes evoked by AP. Practical applications: Acute pancreatitis is characterized by high mortality (average about 5%; severe cases may reach about 30%). The current treatment for acute pancreatitis is mainly symptomatic. The introduction of herbal drugs may lead to the development of a new strategy in the treatment of this disease. This study revealed that MC reduced pancreatic injury by decreasing pro‐inflammatory cytokines, increasing antioxidant capacity and anti‐inflammatory cytokine, IL‐10. To the authors' knowledge, this research is the first report showing that MC inhibits the development of AP. This observation suggests that MC may be useful in the prevention and the treatment of AP in clinical settings. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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29. The effects of riboflavin on ischemia/reperfusion induced renal injury: Role on caspase-3 expression
- Author
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Göksel Şener, Cansu Kandemir, Büşra Ertaş, Feriha Ercan, Zatiye Çevikelli, Ozge Cevik, Betul Ayaz Adakul, Dilek Özbeyli, Tarik Emre Sener, Ayaz Adakul, Betul, Ertas, Busra, Cevikelli, Zatiye Ayca, Ozbeyli, Dilek, Ercan, Feriha, Kandemir, Cansu, Cevik, Ozge, Sener, Tarik Emre, and Sener, Goksel
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ABDOMINAL AORTIC-ANEURYSM ,MELATONIN ,business.industry ,caspase ,Pharmacology toxicology ,Ischemia ,MYELOPEROXIDASE ,Caspase 3 ,Riboflavin ,Pharmacology ,medicine.disease ,Antioxidants ,ISCHEMIA ,Renal injury ,ischemia reperfusion ,medicine ,VITAMIN B-2 ,RAT ,REPERFUSION ,riboflavin ,OXIDATIVE STRESS ,business - Abstract
Reactive oxygen metabolites play important roles in ischemia/reperfusion (I/R) injury in several organ systems. Riboflavin has been shown to exert antioxidant and/or anti-inflammatory activities in several experimental models. The aim of this study was to investigate the role of riboflavin against I/R injury in the rat kidney. Wistar albino rats 200-300 g weighing were divided into 3 groups. One week after unilateral nephrectomy, the IR procedure was applied to the rats. To induce I/R injury renal pedicle was clamped for 45 minutes and then rats were allowed reperfusion for 6 hours. Riboflavin (25 mg/ kg, orally) or vehicle was administered for one week as pretreatment. After decapitation, kidney tissue samples were taken for the evaluation of malondialdehyde (MDA), an end product of lipid peroxidation; glutathione (GSH), a key antioxidant; and 8-hydroxydeoxyguanosine (8-OHdG), a specific marker of oxidative DNA damage. Furthermore, myeloperoxidase (MPO) and caspase-3 activities were also examined together with histological analysis. Ischemia/reperfusion induced significant increases in MDA and 8-OHdG levels and MPO and caspase- 3 activities, and decrese in GSH levels. In the riboflavin treatment these indices were found to be reversed back to control levels. The present data demonstrated that riboflavin, through its antioxidant effect, attenuates I/R induced acute renal injury in rats.
- Published
- 2019
30. Accelerated diabetic wound healing by topical application of combination oral antidiabetic agents-loaded nanofibrous scaffolds: An in vitro and in vivo evaluation study
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Sumeyye Cesur, Levent Kabasakal, Büşra Ertaş, Oguzhan Gunduz, Zehra Demirbas, Christina Katsakouli, Ceyda Ekentok, Ece Guler, Ayse Nur Hazar-Yavuz, Hussain Alenezi, Gul Sinemcan Ozcan, Muhammet Emin Cam, Mehmet S. Eroglu, Dilek Akakin, Mohan Edirisinghe, Cam, Muhammet Emin, Ertas, Busra, Alenezi, Hussain, Hazar-Yavuz, Ayse Nur, Cesur, Sumeyye, Ozcan, Gul Sinemcan, Ekentok, Ceyda, Guler, Ece, Katsakouli, Christina, Demirbas, Zehra, Akakin, Dilek, Eroglu, Mehmet Sayip, Kabasakal, Levent, Gunduz, Oguzhan, and Edirisinghe, Mohan
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Materials science ,food.ingredient ,Combination therapy ,METFORMIN ,Diabetic wound healing ,Nanofibers ,FABRICATION ,Bioengineering ,02 engineering and technology ,Pharmacology ,010402 general chemistry ,01 natural sciences ,Gelatin ,PPAR ,Diabetes Mellitus, Experimental ,Biomaterials ,Chitosan ,Glibenclamide ,chemistry.chemical_compound ,Mice ,DELIVERY ,food ,Dermis ,Pressurized gyration ,In vivo ,HYPERGLYCEMIA ,medicine ,Animals ,Hypoglycemic Agents ,Fibroblast ,CHITOSAN ,Wound Healing ,Tissue Scaffolds ,Pioglitazone ,Electrospinning ,RECEPTOR ,GELATIN ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Rats ,medicine.anatomical_structure ,chemistry ,Mechanics of Materials ,Drug delivery ,0210 nano-technology ,medicine.drug - Abstract
The combination of oral antidiabetic drugs, pioglitazone, metformin, and glibenclamide, which also exhibit the strongest anti-inflammatory action among oral antidiabetic drugs, were loaded into chitosan/gelatin/polycaprolactone (PCL) by electrospinning and polyvinyl pyrrolidone (PVP)/PCL composite nanofibrous scaffolds by pressurized gyration to compare the diabetic wound healing effect. The combination therapies significantly accelerated diabetic wound healing in type-1 diabetic rats and organized densely packed collagen fibers in the dermis, it also showed better regeneration of the dermis and epidermis than single drug-loaded scaffolds with less inflammatory cell infiltration and edema. The formation of the hair follicles started in 14 days only in the combination therapy and lower proinflammatory cytokine levels were observed compared to single drug-loaded treatment groups. The combination therapy increased the wettability and hydrophilicity of scaffolds, demonstrated sustained drug release over 14 days, has high tensile strength and suitable cytocompatibility on L929 (mouse fibroblast) cell and created a suitable area for the proliferation of fibroblast cells. Consequently, the application of metformin and pioglitazone-loaded chitosan/gelatin/PCL nanofibrous scaffolds to a diabetic wound area offer high bioavailability, fewer systemic side effects, and reduced frequency of dosage and amount of drug.
- Published
- 2021
31. Beta vulgaris L. var. cicla improves memory deficits in intracerebroventricular streptozotocin injected rats: Role on neuroinflammation
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Ozlem Sacan, Rezzan Gulhan, Göksel Şener, Büşra Ertaş, Refiye Yanardağ, Fadime Topal, Ertas, Busra, Topal, Fadime, Gulhan, Rezzan, Yanardag, Refiye, Sacan, Ozlem, and Sener, Goksel
- Subjects
Chemistry ,Extract ,EXTRACT ,CHOLINESTERASE ,Cholinesterase ,Pharmacology ,Alzheimer's disease ,neuroinflammation ,ALZHEIMERS-DISEASE ,Oxidative Stress ,Intracerebroventricular streptozotocin ,OXIDATIVE STRESS ,Beta (finance) ,Beta vulgaris L. var. cicla ,Neuroinflammation ,cholinergic dysfunction ,cognitive function ,Alzheimers-Disease - Abstract
Alzheimer's disease is a challenging disease for patients due to progressive loss of cognition and behavioral disorders. Disruption of cholinergic transmission and neuroinflammation are the most important mechanisms underlying cognitive damage. Beta vulgaris L. var. cicla (BV) has been reported to have various pharmacological effects associated with its rich antioxidant content. In addition, anti-cholinesterase and antiinflammatory activities of BV have been demonstrated in vitro. The aim of this study is to elucidate the therapeutic effect of BV against cognitive impairment, reduction in cholinergic transmission and neuroinflammation caused by intracerebroventricular (ICV) administration of streptozotocin (STZ). STZ was administered bilaterally at a dose of 3 mg/kg via ICV to rats, and BV treatment at a dose of 2 g/kg for 21 days was administered orally to STZ-induced animals. After behavioral tests, AChE activity, TNF-alpha and IL-1 beta levels were measured in hippocampus and cortex tissues excised from decapitated animals. Novel object recognition and passive avoidance test showed that the treatment of BV reverted the ICV-STZ induced memory dysfunctions in rats. Furthermore, increased AChE levels in the hippocampal and cortical tissues of STZ-induced rats were significantly reduced with 21 days of BV treatment. In conclusion, these results confirm that STZ administration caused cholinergic hypofunction, neuronal inflammation and cognitive dysfunction in rats, and BV therapy significantly inhibited these changes with its potential neuroprotective activity.
- Published
- 2021
32. The comparision of glybenclamide and metformin-loaded bacterial cellulose/gelatin nanofibres produced by a portable electrohydrodynamic gun for diabetic wound healing
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Gul Sinemcan Ozcan, Yusufhan Yazir, Ayse Nur Hazar-Yavuz, Ceyda Ekentok, Ece Guler, Muhammet Emin Cam, Büşra Ertaş, Maryam Crabbe-Mann, Hussain Alenezi, Fadime Topal, Mohan Edirisinghe, Maryam Parhizkar, Cam, Muhammet Emin, Crabbe-Mann, Maryam, Alenezi, Hussain, Hazar-Yavuz, Ayse Nur, Ertas, Busra, Ekentok, Ceyda, Ozcan, Gul Sinemcan, Topal, Fadime, Guler, Ece, Yazir, Yusufhan, Parhizkar, Maryam, and Edirisinghe, Mohan
- Subjects
food.ingredient ,Polymers and Plastics ,General Physics and Astronomy ,02 engineering and technology ,Pharmacology ,010402 general chemistry ,PHENOTYPE ,01 natural sciences ,Gelatin ,Bacterial cellulose ,chemistry.chemical_compound ,food ,In vivo ,Materials Chemistry ,medicine ,Cytotoxicity ,GELATIN ,Organic Chemistry ,Oral antidiabetic agents ,Granulation tissue ,Drug release ,Portable electrohydrodynamic gun ,021001 nanoscience & nanotechnology ,Electrospinning ,0104 chemical sciences ,Bioavailability ,Metformin ,medicine.anatomical_structure ,Wound dressings ,chemistry ,0210 nano-technology ,SKIN ,medicine.drug - Abstract
Wound dressings made from natural polymers are an important aspect of biomaterials. Protein-based materials are less likely to instigate an immunogenic response and have the capacity to degrade in vivo, also without triggering an inflammatory response. Therefore, gelatin (GEL) was chosen and combined with bacterial cellulose (BC) to produce nanofibres and the potential of an all-natural polymer construct was determined. GEL and BC were successfully electrospun with metformin (Met) and glybenclamide (Gb) using a portable, point of need electrospinning set up. The virgin fibre group exhibited a significant effect on the proliferation of L929 (mouse fibroblast) cells but all fibre samples can safely be applied on wound site without risk of cytotoxicity. According to the results obtained by animal tests, the GEL-BC-Gb group showed better recovery than the GEL-BC-Met group. Diabetic wounds treated with GEL-BC-Met were characterized by moderate re-epithelialization and partially organized granulation tissue. Moderate to complete re-epithelialization and well-formed granulation tissue were observed in diabetic wounds treated with GEL-BC-Gb. The histologic scores obtained on day 14 confirmed that the GEL-BC-Gb group played a stronger wound-healing role compared to the GEL-BC-Met group. The highest decrease of TNF-α level was observed in the GEL-BC-Gb group at the end of the experiment but there is no significant difference between drug-loaded fibre groups. Therefore, topical administration of Met and Gb in a sustained release form has a high potential for diabetic wound healing with high bioavailability and fewer systemic side effects but Gb showed better improvement according to the results of the animal tests.
- Published
- 2020
33. The effects of Urtica dioica L. ethanolic extract against urinary calculi in rats
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Pinar Eker, Ahmet Doğan, Tarik Emre Sener, Büşra Ertaş, Damla Gokceoglu Kayali, Göksel Şener, Ali Şen, Rumeysa Keles, Şule Çetinel, Keles, Rumeysa, Sen, Ali, Ertas, Busra, Kayali, Damla, Eker, Pinar, Sener, Tarik Emre, Dogan, Ahmet, Cetinel, Sule, and Sener, Goksel
- Subjects
antioxidant ,NEPHROTOXICITY ,Traditional medicine ,business.industry ,Urinary system ,Pharmacology toxicology ,urolithiasis ,NEPHROLITHIASIS ,Urtica dioica ,GLYCOL-INDUCED UROLITHIASIS ,kidney stone ,KIDNEY ,INFLAMMATION ,calcium oxalate ,INJURY ,Medicine ,business - Abstract
Nephrolithiasis is common urological problem and stone formation has multiple underlying pathogenetic factors. We investigated the possible preventive and therapeutic effect of Urtica dioica ethanol extract (UD) on ethylene glycol-induced nephrolithiasis model in rats. Sprague-Daw ley rats were divided into lour groups (n = 10). The control group was given normal drinking water for 8 weeks and was administered vehicle by gastric gavage. Stone formation was induced by adding 0.75% ethylene glycol (EG) to their drinking water. UD (700 mg/kg) was given orally lor 8 weeks to the preventive group and I or last 4 weeks to the treatment respectively. At the end of the experiment, urine, blood samples and kidney tissues were obtained. In 24-hour urine samples, calcium and citrate levels were decreased and oxalate levels were increased in EG whereas LID treatment groups reversed these parameters back to control levels. In addition, serum levels of creatinine and urea were increased in EG while LID significantly reduced these parameters. Malondialdehyde, 8-hydroxydeoxyguanosine and tumor necrosis alpha levels, and caspase- 3 and N-acetyl-beta-glucosaminidase activities were elevated in EG group and showed a decrease in LID treated groups. Glutathione level was decreased in EG group, whereas it was increased in UD preventive group. Histological examination showed an improvement in UD treated groups. Our results suggest that UD is effective both in prevention and treatment for kidney stones. The mechanism underlying this effect may be the antioxidant effect of UD and the effect on the concentration of stone-forming components in the urine.
- Published
- 2020
34. Nicorandil preserves blood-brain barrier and improves memory impairment in hypertensive rats
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Berrak Ç. Yeğen, Göksel Şener, Türkan Koyuncuoğlu, Zatiye Ayça Çevikelli Yakut, Büşra Ertaş, Cevikelli Yakut, Zatiye Ayca, Ertas, Busra, Koyuncuoglu, Turkan, Yegen, Berrak C., and Sener, Goksel
- Subjects
EXPRESSION ,STIMULATION ,medicine.medical_specialty ,NF-KAPPA-B ,blood brain barrier ,Blood–brain barrier ,SUPEROXIDE ,ACTIVATION ,INFLAMMATION ,Internal medicine ,medicine ,Memory impairment ,PERMEABILITY ,Nicorandil ,Renovascular hypertension ,RECEPTOR ,business.industry ,angiotensin 2 ,II-INDUCED HYPERTROPHY ,vascular dementia ,nicorandil ,medicine.anatomical_structure ,Cardiology ,RHO-KINASE ,business ,medicine.drug - Abstract
In renovascular hypertension (RVH), oxidative stress and inflammation due to high blood pressure and elevated levels of angiotensin 2 are mainly responsible of cerebrovascular complications and impaired cognitive functions. Since the nicorandil has been shown to exert neuroprotective, anti-inflammatory and antioxidant effects, we investigated the effect of nicorandil against vascular dementia and blood brain barrier damage in a rat model of angiotensin-dependent hypertension. Wistar albino rats, were divided as sham-operated control, renovascular hypertension (RVH) and Nicorandil-treated RVH groups. Silver clip was implanted onto the left renal artery. Using the tail-cuff method, blood pressure of rats was measured before the surgery and at the end of the post-surgical 3rd and 12th weeks. Nicorandil (4mg/kg, orally) or vehicle was administered for 9 weeks. Twelve weeks after RVH surgery, a new object recognition test was performed. Following the determination of blood brain barrier integrity, serum samples were taken for the evaluation of proinflammatory cytokines tumor necrosis alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta). Levels of sodium-potassium adenosine triphosphatase (Na+/K+-ATPase), as a marker of endothelial damage, were evaluated in the hippocampal tissues. RVH resulted in significant increases in TNF-alpha and IL-1 beta levels and decreases in Na+/K+-ATPase levels, along with impairment in blood brain barrier integrity and memory performance. In the nicorandil treatment group, these indices were reversed back to control levels. The present data demonstrated that nicorandil attenuates RVH-induced memory impairment and blood brain barrier damage in rats with RVH.
- Published
- 2019
35. Ameliorative effects of riboflavin on acetic acid-induced colonic injury in rats
- Author
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Göksel Şener, Berna Karakoyun, Meral Yüksel, Ozge Cevik, Büşra Ertaş, Dilek Akakin, and [Karakoyun, Berna] Marmara Univ, Dept Basic Hlth Sci, Fac Hlth Sci, Istanbul, Turkey -- [Ertas, Busra -- Sener, Goksel] Marmara Univ, Dept Pharmacol, Fac Pharm, Istanbul, Turkey -- [Yuksel, Meral] Marmara Univ, Vocat Sch Hlth Related Profess, Dept Med Lab, Istanbul, Turkey -- [Akakin, Dilek] Marmara Univ, Sch Med, Dept Histol & Embryol, Istanbul, Turkey -- [Cevik, Ozge] Cumhuriyet Univ, Dept Biochem, Fac Pharm, Sivas, Turkey
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0301 basic medicine ,medicine.medical_specialty ,Time Factors ,Antioxidant ,Colon ,colitis ,Physiology ,Riboflavin ,medicine.medical_treatment ,oxidative damage ,Collagen Type I ,Rats, Sprague-Dawley ,Transforming Growth Factor beta1 ,03 medical and health sciences ,chemistry.chemical_compound ,Western blot ,Sulfasalazine ,Physiology (medical) ,Internal medicine ,medicine ,Animals ,Deoxyguanosine ,riboflavin ,Colitis ,Acetic Acid ,Peroxidase ,Pharmacology ,Dose-Response Relationship, Drug ,medicine.diagnostic_test ,biology ,Chemistry ,Organ Size ,Glutathione ,medicine.disease ,Rats ,Collagen Type I, alpha 1 Chain ,Collagen Type III ,acetic acid ,030104 developmental biology ,Endocrinology ,Cytoprotection ,Myeloperoxidase ,biology.protein ,Female ,medicine.drug - Abstract
WOS: 000433569300009, PubMed ID: 29164668, Riboflavin (RF) has been found to be a promising antioxidant and/or anti-inflammatory agent in several studies. However, the effect of RF against acetic acid (AA)-induced colonic injury is currently unknown. This study aimed to investigate the potential antioxidant and protective effects of RF in a rat model of ulcerative colitis. Starting immediately after the colitis induction (AA+RF group) or 1week before the colitis induction (RF+AA+RF group), the rats were treated with RF (25mg/kg per day; p.o.) for 3days. The control and AA groups received saline (1mL; p.o.) whereas AA+SS group (positive control) received sulfasalazine (100mg/kg per day; p.o.) for 3days. Colonic samples were taken for the biochemical and histological assessments on the third day. High damage scores, elevated tissue wet weight index (WI), tissue myeloperoxidase (MPO) activity, 8-hydroxy-2-deoxyguanosine levels and chemiluminescence values, and a pronounced decrease in antioxidant glutathione (GSH) levels of the AA group were all reversed by RF pretreatment (RF+AA+RF group) and SS treatment (AA+SS group) (P
- Published
- 2017
36. Antidiabetic effects of Salvia triloba and Thymus praecox subsp. skorpilii var. skorpilii in a rat model of streptozotocin/nicotinamide-induced diabetes
- Author
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Muhammet Emin Cam, Levent Kabasakal, Ayşe Eda Acar, Turgut Taşkın, Sila Yildiz, Büşra Ertaş, Cam, Muhammet Emin, Yildiz, Sila, Ertas, Busra, Acar, Ayse Eda, Taskin, Turgut, and Kabasakal, Levent
- Subjects
endocrine system diseases ,Type II diabetes mellitus ,Rat model ,02 engineering and technology ,Pharmacology ,Salvia ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Diabetes mellitus ,medicine ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Salvia triloba ,Traditional medicine ,biology ,Nicotinamide ,Chemistry ,antidiabetic ,ALLOXAN ,Insulin tolerance test ,Streptozotocin nicotinamide ,021001 nanoscience & nanotechnology ,medicine.disease ,biology.organism_classification ,Streptozotocin ,0104 chemical sciences ,Thymus praecox ,herbal medicines ,Lamiaceae ,Streptozotocin-Nicotinamide ,0210 nano-technology ,medicine.drug - Abstract
Some Salvia and Thymus species of Lamiaceae family come into prominence with strong antidiabetic effects. Compared to the other species, there are limited studies on antidiabetic activity of Salvia triloba (ST) and Thymus praecox subsp. skorpilii var. skorpilii (TPS). The aim of this study was to adjust the dosage and to determine the antidiabetic effects of methanol extracts of ST and TPS in streptozotocin/nicotinamide-induced diabetic rats. Type II diabetes mellitus (T2DM) was induced by a single intraperitoneal (i. p.) injection of streptozotocin (STZ) dissolved in 0.1 M cold citrate buffer (pH 4.5) at a dose of 55 mg/kg/body weight (b.w.) and nicotinamide (100 mg/kg/b.w.) was given prior to STZ injection. For adjusting dosage, oral glucose tolerance test (OGTT) was used while insulin tolerance test (ITT), OGTT, blood glucose levels and animal weights were used to evaluate the antidiabetic effects of ST and TPS. According to the OGTT, the most effective doses for ST and TPS were 200 mg/kg and 100 mg/kg, respectively. At the end of three weeks, blood glucose levels of control goup reached to 462.50 mg/dl, whereas ST and TPS-treated groups blood glucose levels decreased less than 200.00 mg/dl. In conclusion, the present study suggests that both of ST and TPS methanolic extracts may be of therapeutic benefit in diabetes and thus need to further studies.
- Published
- 2017
37. Ethanolic extract of cotinuscoggygria leaves attenuates crystalluria and kidney damage in ethylene glycol-induced urolithiasis in rats.
- Author
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Gumru S, Ozgur G, Ertas B, Sen A, Eker P, Sener TE, and Sener G
- Abstract
Objective: Nephrolithiasis is a common cause of kidney insufficiency. Nephrolithiasis is proven to be the result of various biochemical and inflammatory processes that result in crystal formation and subsequent aggregation. Cotinuscoggygria L. (CCog) is a plant extract which has been used as a Turkish remedy for kidney stones. With this study, we planned to evaluate the effects of CCog extract in ethylene glycol (EG)-induced nephrolithiasis model in rats., Methods: The study group comprised 32 Wistar albino rats which were divided into Control (C), EG, CCog Prophylaxis (CC+EG+CC), and CCog Treatment (EG+CC) groups. Stone formation was induced by adding EG (0.75%) into rat's drinking water. Normal drinking water was given to Control group for 8 weeks. Throughout the study period of 8 weeks, EG group was given only EG (0.75%) and CC+EG+CC group was given both EG and CCog. In EG+CC group, EG (0.75%) was given for 8 weeks whereas CCog was given for the past 4 weeks. After the 8
th week, 24-h urine samples were collected. Rats were then sacrificed and kidney tissue samples were harvested., Results: Metabolites (calcium, citrate) and creatinine in 24 h urine samples were decreased in CC+EG+CC and EG+CC groups. While hyperoxaluria was observed in the EG group, oxalate levels were similar to control levels in the P-CCog and C-CCog groups. The N-acetyl-β-glucosaminidase and myeloperoxidase activities were both increased in EG group and these parameters were significantly decreased on CCog treatment., Conclusion: We can conclude that C. coggygria extract can have beneficial effect on lowering concentration of stone-forming metabolites in urine and consequently protect renal tissues from damage due to nephrolithiasis. C. coggygria extract can be considered as a potential prophylactic and therapeutic option in high-risk stone formers. Furthermore, our data confirm ethnobotanical use of CC against nephrolithiasis., Competing Interests: No conflict of interest was declared by the authors., (© Copyright 2023 by Istanbul Provincial Directorate of Health.)- Published
- 2023
- Full Text
- View/download PDF
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