1. Putative Protein Biomarkers of Escherichia coli Antibiotic Multiresistance Identified by MALDI Mass Spectrometry
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Bardot, Valérie, Escalon, Anaïs, Ripoche, Isabelle, Denis, Sylvain, ALRIC, Monique, Chalancon, Sandrine, Chalard, Pierre, Cotte, César, Berthomier, Lucile, Leremboure, Martin, Dubourdeaux, Michel, Universidade de Trás-os-Montes e Alto Douro (UTAD), Universidade do Trás-os-Montes e Alto Douro, Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Microbiologie Environnement Digestif Santé (MEDIS), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), 'Espectrometria de massa e sequenciacao aplicadas a microbiologia clinica moderna: pesquisa e identificacao de biomarcadores em especies bacterianas multiresistentes' (Programa de Accoes Universitarias Integradas Luso-Francesas) TC-14/2017Associate Laboratory for Green Chemistry-LAQV - FCT/MCTES UIDB/50006/2020, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), Tissus animaux, nutrition, digestion, écosystème et métabolisme (TANDEM), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-INP. Ecole Nationale Supérieure Agronomique de Toulouse, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Institut National de la Recherche Agronomique (INRA), Université de Pau et des Pays de l'Adour (UPPA), Veterinary Science Department, Faculty of Science and Technology, University of Trás-os-Montes and Alto Douro (Portugal), Department of Genetics and Biotechnology, University of Tras-os-Montes and Alto Douro, LAQV@REQUIMTE, DQ - Departamento de Química, PiLeJe Industrie, Sigma CLERMONT (Sigma CLERMONT), Microbiologie Environnement Digestif Santé - Clermont Auvergne (MEDIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Equipe MaQuaV, INATAA, Université Mentouri Constantine [Algérie] (UMC), Université Clermont Auvergne (UCA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Équipe Transformation et Elaboration des Produits Agro-Alimentaires (TEPA), Laboratoire de Nutrition et Technologies Alimentaires (LNTA)
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0301 basic medicine ,medicine.drug_class ,Tetracycline ,[SDV]Life Sciences [q-bio] ,030106 microbiology ,Antibiotics ,Virulence ,medicine.disease_cause ,Bacterial resistance ,Article ,General Biochemistry, Genetics and Molecular Biology ,antibiotics ,Microbiology ,03 medical and health sciences ,one health ,Antibiotic resistance ,SDG 3 - Good Health and Well-being ,Immunology and Microbiology(all) ,Ampicillin ,medicine ,MALDI-TOF MS ,lcsh:QH301-705.5 ,Gene ,Escherichia coli ,One health ,Agricultural and Biological Sciences(all) ,General Immunology and Microbiology ,biology ,Biochemistry, Genetics and Molecular Biology(all) ,bacterial resistance ,biomarkers ,biology.organism_classification ,3. Good health ,030104 developmental biology ,lcsh:Biology (General) ,General Agricultural and Biological Sciences ,Biomarkers ,Bacteria ,medicine.drug - Abstract
Ação n.º: TC-14/2017 UIDB/50006/2020 The commensal bacteria Escherichia coli causes several intestinal and extra-intestinal diseases, since it has virulence factors that interfere in important cellular processes. These bacteria also have a great capacity to spread the resistance genes, sometimes to phylogenetically distant bacteria, which poses an additional threat to public health worldwide. Here, we aimed to use the analytical potential of MALDI-TOF mass spectrometry (MS) to characterize E. coli isolates and identify proteins associated closely with antibiotic resistance. Thirty strains of extended-spectrum beta-lactamase producing E. coli were sampled from various animals. The phenotypes of antibiotic resistance were determined according to Clinical and Laboratory Standards Institute (CLSI) methods, and they showed that all bacterial isolates were multi-resistant to trimethoprim-sulfamethoxazole, tetracycline, and ampicillin. To identify peptides characteristic of resistance to particular antibiotics, each strain was grown in the presence or absence of the different antibiotics, and then proteins were extracted from the cells. The protein fingerprints of the samples were determined by MALDI-TOF MS in linear mode over a mass range of 2 to 20 kDa. The spectra obtained were compared by using the ClinProTools bioinformatics software, using three machine learning classification algorithms. A putative species biomarker was also detected at a peak m/z of 4528.00. publishersversion published
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- 2020