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Chicken meat is one of the most consumed proteins worldwide, however, it is one of the main reservoirs of Escherichia coli which is the main cause of multiple infections after consumption due to different factors such as inefficient handling at the time of slaughter, poor hygiene and inadequate refrigeration. The diseases it causes not only affect the health of consumers, but also the economy of traders. Therefore, this study aimed to identify by sequencing the 16S rRNA gene different strains that were isolated in chicken meat, for this 135 samples were taken and collected from formal and informal points of sale and from 9 slaughtering centers that supply Ambato. The samples were evaluated by means of microbiological and biochemical tests and then DNA was extracted from the 40 suspect samples, which were analyzed by PCR. The results were obtained through sequencing where a total of 33 different strains were identified, strains AF1 and ATCC 11775 were predominant in the chicken meat samples in the canton of Ambato. [ABSTRACT FROM AUTHOR]