175 results on '"Escudero, Raquel"'
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2. Executive summary of the consensus statement of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC), Spanish Society of Neurology (SEN), Spanish Society of Immunology (SEI), Spanish Society of Pediatric Infectology (SEIP), Spanish Society of Rheumatology (SER), and Spanish Academy of Dermatology and Venereology (AEDV), on the diagnosis, treatment and prevention of Lyme borreliosis
- Author
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Oteo, José A., Corominas, Héctor, Escudero, Raquel, Fariñas-Guerrero, Fernando, García-Moncó, Juan Carlos, Goenaga, Miguel A., Guillén, Sara, Mascaró, José M., and Portillo, Aránzazu
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- 2023
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3. Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays
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Nakajima, Rie, Escudero, Raquel, Molina, Douglas M, RodrÃguez-Vargas, Manuela, Randall, Arlo, Jasinskas, Algis, Pablo, Jozelyn, Felgner, Philip L, AuCoin, David P, Anda, Pedro, Davies, D. Huw, and Carroll, K. C
- Published
- 2016
4. Strengthening the genomic surveillance of Francisella tularensis by using culture-free whole-genome sequencing from biological samples
- Author
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Isidro, Joana, primary, Escudero, Raquel, additional, Luque-Larena, Juan José, additional, Pinto, Miguel, additional, Borges, Vítor, additional, González-Martín-Niño, Rosa, additional, Duarte, Sílvia, additional, Vieira, Luís, additional, Mougeot, François, additional, Vidal, Dolors, additional, Herrera-Rodríguez, Daniel, additional, Rodríguez-Pastor, Ruth, additional, Herrero-Cófreces, Silvia, additional, Jubete-Tazo, Fernando, additional, Gomes, João Paulo, additional, and Lopes de Carvalho, Isabel, additional
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- 2024
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5. Procalcitonin (PCT) levels for ruling-out bacterial coinfection in ICU patients with influenza: A CHAID decision-tree analysis
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Cobo, Pedro, Martins, Javier, Carbayo, Cecilia, Robles-Musso, Emilio, Cárdenas, Antonio, Fierro, Javier, Fernández, Dolores Ocaña, Sierra, Rafael, Huertos, Mª Jesús, Carmona Pérez, Mª Luz, Pozo Laderas, Juan Carlos, Guerrero, R., Robles, Juan Carlos, León, Melissa Echevarría, Gómez, Alberto Bermejo, Márquez, Enrique, Rodríguez-Carvajal, Manuel, Estella, Ángel, Pomares, José, Ballesteros, José Luis, Romero, Olga Moreno, Fernández, Yolanda, Lobato, Francisco, Prieto, José F., Albofedo-Sánchez, José, Martínez, Pilar, de la Torre, María Victoria, Nieto, María, Sola, Estefanía Camara, Díaz Castellanos, Miguel Angel, Soler, Guillermo Sevilla, Leyba, Carlos Ortiz, Garnacho-Montero, José, Hinojosa, Rafael, Fernández, Esteban, Loza, Ana, León, Cristóbal, López, Samuel González, Arenzana, Angel, Ocaña, Dolores, Navarrete, Inés, Beryanaki, Medhi Zaheri, Sánchez, Ignacio, Pérez Alé, Manuel, Poullet Brea, Ana Mª, Machado Casas, Juan Francisco, Serón, Carlos, Avellanas, Manuel Luis, Lander, Arantxa, de Arellano, S. Garrido Ramírez, Lacueva, M.I. Marquina, Luque, Pilar, Serrano, Elena Plumed, Martín Lázaro, Juan Francisco, Polo, Carlos Sánchez, Cia, Isabel Gutiérrez, Bartolomé, Belén Jiménez, Nuñez, Carlos López, González, Ignacio, Tomás Marsilla, José Ignacio, Andrés, Clara Jaques, Ibañes, Pablo Gtiérrez, Aguilar, Pilar Araujo, Montón, Jose Mª, Regil, Paloma Dorado, Iglesias, Lisardo, González, Carmen Pascual, Fernández, Brígida Quindós, Iglesias, Lorena Martín, Soria, Lucía Viña, Escudero, Raquel Yano, Revuelta, Mª del Rosario Mtnez, Quiroga, García-Rodríguez, Águeda, Cuadrado, Marta Martín, Balán Mariño, Ana Luz, Socias, Lorenzo, Ibánez, Pedro, Borges-Sa, Marcío, Socias, A., Del Castillo, A., Marcos, Ricard Jordà, Muñoz, Cristina, Bonell, José M., Amestarán, Ignacio, López, M. Angeles González, Pàmies, Cecilia Vilanova, Bonell Goytisolo, José Ma., Morales Carbonero, José Antonio, Senoff, Rossana Pérez, López de Medrano, Marta Generelo, Ruiz-Santana, Sergio, Díaz, Juan José, Ramírez, Catalina Sánchez, Sisón, Montse, Hernández, David, Trujillo, Ana, Regalado, Luis, Fndez, Sonia Rodríguez, Lorente, Leonardo, Rivero, Judith Cabrera, Mora Quintero, Mª Luisa, Martín, Mar, Martínez, Sergio, Cáceres, J.J., Sanchez Palacio, Manuel, Marcos, García Rodríguez, D., Leria, María Ripoll, Suberviola, Borja, Ugarte, P., García-López, Fernando, Iniesta, Rafael Sánchez, Alonso, Angel Álvaro, Padilla, Antonio, Palacios, Basi Martínez, Grande, Mª Luisa Gómez, Martín Rodríguez, Ma. Carmen, Adbel-Hadi Álvarez, Hasania, Ambros Checa, Alfonso, Hernández, Higinio Martín, Albaya, Antonio, Obregón, Alberto Silva, Crespo, Carlos Marian, Estrella, Carlos Armendariz, Benito Puncel, Carmen, Oyargue, Eduardo Quirós, Canabal, Alfonso, Marina, Luis, López de Toro, Ismael, Simón, Almudena, Añón, José María, López Messa, Juan B., López Pueyo, Mª Jesús, del valle Sergio Ossa Echeverri, Ortíz María, Ferreras, Zulema, Ballesteros Herraez, Juan C., Macias, Santiago, Berezo, José Ángel, Varela, Jesús Blanco, Schweizer, Pablo Blanco, Salamanca, Angela González, Lomas, Luis Tamayo, Anzález, Andaluz Ojeda, Cicuéndez Avila, Ramón, Francisco Javier, Pérez G., Terrero, Antonio Álvarez, Ezpeleta, Fabiola Tena, Sala, Christian, López, Oliverio, Paez, Zulema, García, Álvaro, Carriedo Ule, Demetrio, Crespo, Miriam Riesco, Rebolledo, Jesús Pino, Andrés, Nicolás Hidalgo, Zirena, Ana Carolina Caballero, García, Belén Román, López Messa, Juan Bautista, del Valle Ortiz, María, Echeverri, Sergio Ossa, Catalán, Rosa Mª, Ferrer, Miquel, Torres, Antoni, Cilloniz, Catia, Ansorregui, Sandra Barbadillo, Cabré, Lluís, Baeza, Ignacio, Rovira, Assumpta, Álvarez-Lerma, Francisco, Vázquez, Antonia, Nolla, Joan, Fernández, Francisco, Cervelló, Joaquim Ramón, Iglesia, Raquel, Mañéz, Rafael, Ballús, J., Granada, Rosa Mª, Vallés, Jordi, Díaz, Emili, Ortíz, Marta, Guía, C., Martín-Loeches, Ignacio, Páez, Joaquim, Almirall, Jordi, Balanzo, Xavier, Güell, Estel, Yebenes, Juan Carlos, Rello, Jordi, Arnau, Elena, Pérez, Marcos, Laborda, César, Souto, Jesica, Lagunes, Leonel, Catalán, Iñaki, Sirvent, Josep Mª, de Arbina, Nerea López, Serra, Anna Baró, Sánchez, Adriana, Cuenca;, Silvia M., Badía, Mariona, Baseda-Garrido, Begonia, Valverdú-Vidal, Montserrat, Barcenilla, Fernando, Palomar, Mercedes, Nuvials, Xavier, Benedicto, Pedro Garrido, Campo, Ferran Roche, Esteban, M.F., Luna, José, Eixarch, Gaspar Masdeu, Diago, Angels Pascual, Nava, Juan Mª, González de Molina, J., Trenado, Josep, Ferrer, Ricard, Josic, Zoran, Casanovas, Montserrat, Gurri, Francisco, Rodríguez, Paula, Rodríguez, Alejandro, Claverias, Laura, Trefler, Sandra, Bodí, María, Magret, Mónica, Ferri, Cristina, Díaz, Rosa María, Mesalles, Eduard, Arméstar, Fernando, de Mendoza, Diego, Fernández, Carmen Lomas, Berrade, José Julián, Saris, Alfonso Bonet, Pechkova, Marina, Jiménez, Cristina Mora, Gil, Santiago Picos, Juliá-Narváez, José, Marcos, Manuel Robles, Mallqui, Vanessa Farje, Santiago Triviño, Mª. Angeles, García, Pablo Martínez, Fernández-Zapata, Alberto, Recio, Teresa, Arrascaeta, Abilio, García-Ramos, Mª José, Gallego, Elena, Rodrigo, Esther Saiz, Bueno, Fernándo, Díaz, Mercedes, Pérez, Noemí Gil, Hormigo, David López, Delgado, Juan Diego Jiménez, Frutos, Pérez, Rivera Pinna, M.J., Cordero, Mª Lourdes, Pastor, José A., Álvarez-Rocha, Luis, Ceniceros Barros, Alexandra, Pedreira, Alejandra Virgós, Vila, Dolores, González, Carmen Fernández, Pérez, Javier Blanco, Piquer, M. Ortiz, Merayo, Eleuterio, López-Ciudad, Victor Jose, Cañones, Juan Cortes, Vilaboy, Eva, Chao, José Villar, Cid López, Francisco Savira, Cortés, Pablo Vidal, Pérez Veloso, Marcos A., Saborido, Eva Maria, Pardavila, Enrique Alemparte, Montes, Ana Ortega, González, Raul José, Freita, Santiago, Alemparte, Enrique, Ortega, Ana, López, Ana María, Canabal, Julio, Ferres, Enrique, Ramos, Santiago Freitas, Cendón, Lucas Lage, Casal, Vanesa Gómez, Adrio, Sabela Vara, Fernández, Eva Menor, Prado, Susana González, Franco, Antonio Varela, Monzón, José Luis, Goñi, Félix, Del Nogal Sáez, Frutos, Navalpotro, M. Blasco, Abad, Ricardo Díaz, Lasierra, José Luis Flordelis, García-Torrejón, Mª Carmen, Pérez–Calvo, César, López, Diego, Arnaiz, Luis, Sánchez-Alonso, S., Velayos, Carlos, del Río, Francisco, González, Miguel Ángel, Nieto, Mercedes, Cesteros, Carmen Sánchez, Martín, María Cruz, Molina, José Mª, Montejo, Juan Carlos, Catalán, Mercedes, Albert, Patricia, de Pablo, Ana, Guerrero, José Eugenio, Zurita;, María, Peyrat, Jaime Benitez, Cámara, Miriam Díaz, Cerdá, Enrique, Alvarez, Manuel, Pey, Carlos, Riestra, Eva Manteiga, Martinez-Fidalgo, Concepción, Rodríguez, Montse, Palencia, Eduardo, Caballero, Rafael, Vaquero, Concepción, Mariscal, Francisco, García, S., Cepeda, Rico, Carrasco, Nieves, Prieto, Isidro, Liétor, A., Ramos, R., Casas, Rosario Cuadra, Cuesta, Cruz Soriano, Sánchez Alonso, Susana, Galván, Beatriz, Figueira, Juan C., Soriano, M. Cruz, Martín, Bélen Civantos, Caballero, Alejgandro Robles, Galdós, P., Moreno, Bárbara Balandin, Alcántara Carmona, Sara, del Cabo, Fernández, Hermosa, Cecilia, Gordo, Federico, Algora, Alejandro, Paredes, Amparo, Carmona, Teodoro Grau, Cambroner, J.A., Ramos, Esther López, de Zárate, Yaiza Ortiz, Gómez-Rosado, Sonia, Lodo, Margarita Mas, Garrobo, Nieves Franco, Hernández, Silvia Álvarez, Honrubia, Teresa, Prado López, Luis Miguel, Esteban, A., Lorente, J.A., Nin, N., Sotomayor, Carlos Jaramillo, Silvero, Esperanza Molero, de la Reguera, Eduardo Morales Fdez., de la Casa Monje, Rosa Mª, Serrano, Fátima Martín, Trasmonte Martínez, Mª Victoria, Martín Delgado, M. Cruz, Martínez, Sofía, Abad, F. Felices, Navalon, Isabel Cremades, Velis, Martín Vigil, Martínez, Mariano, Martínez Baño, Domingo, Andreu, Enriqueta, Butí, Sergio Manuel, Rueda, Bernardo Gil, García, Francisco, Fernández, Noemí Llamas, Para, Luis Herrera, Freire, Alejandro Ortín, Nvarro Ruiz, Mª Rosa, Romero, C.R. Hernández, Maraví-Poma, Enrique, Urra, I. Jimenez, Redin, Laura Macaya, Tellería, A., Insansti, Josu, Garcia, Noelia Artesero, Macaya, Laura, Palanco, Joaquín Lobo, González, Nagore, Marco, Pilar, Vidaur, Loreto, Salas, Estibaliz, Udabe, Ruth Salaberría, Santamaría, B., Rodríguez, Tomás, Vergara, Juan Carlos, Amiano, Jose Ramon Iruretagoyena, Santos, Iratí Garrido, Manzano, Alberto, Arenal, Carlos Castillo, Olaechea, Pedro María, López, Alejandro Martín, San Miguel, Fernando Fonseca, Blanquer, José, Carbonell, Nieves, Franco, José Ferreres, Valero, Roberto Reig, Belenger, A., Altaba, Susana, Álvarez–Sánchez, Bernabé, Robles, José Canovas, Francisco, Jaime Sánchez, Sánchez, Mar Ruiz, Picos, Santiago Alberto, Llanes, Abilio Arrascaeta, Gutiérrez, Eugenio Herrero, Zapata, Alberto Fernández, Sánchez-Miralles, Ángel, Antón Pascual, José Luis, Bonastre, Juan, Palamo, M., Cebrian, Javier, Cuñat, José, Sahuquillo, Mónica Gordón, Romero, Belén, Pallé, Santiago Borrás, de León Belmar, Javier, Zaragoza, Rafael, Tormo, Constantino, Chinesta, Susana Sancho, Paricio, Virgilio, Marques, Asunción, Sánchez-Morcillo, S., Tormo, S., Latour, J., García, M. Ángel, Palomo, Manuel, Royo, Francisco Tarín, Hinojosa, Pedro Manzano, Sánchez Pino, Mª Salomé, Ribes, Concha Maragues, Luis, Rubén González, Ribas, Antoli, Rodríguez, Alejandro H., Avilés-Jurado, Francesc X., Schuetz, Philipp, Trefler, Sandra I., Solé-Violán, Jordi, Cordero, Lourdes, Pozo Laderas, Juan C., Vergara, Juan C., Guerrero, José E., Cilloniz, Catia., and Restrepo, Marcos I.
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- 2016
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6. Zoonotic Bacteria in Fleas Parasitizing Common Voles, Northwestern Spain
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Rodriguez-Pastor, Ruth, Mougeot, Francois, Vidal, Ma Dolors, Jado, Isabel, Gonzalez-Martin-Nino, Rosa M., Escudero, Raquel, and Luque-Larena, Juan Jose
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Bacteria ,Disease transmission ,Fleas ,Zoonoses - Abstract
A dynamic prevalence of Francisella tularensis and Bartonella spp. was reported in irruptive common vole (Microtus arvalis) populations during 2013-2015 from agricultural landscapes of northwestern Spain (1,2). In that area, [...]
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- 2019
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7. Strengthening the genomic surveillance of Francisella tularensis by using culture-free whole-genome sequencing from biological samples
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European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Programa Operacional do Potencial Humano (Portugal), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Isidro, Joana, Escudero, Raquel, Luque-Larena, Juan José, Pinto, Miguel, Borges, Vítor, González Martín-Niño, Rosa, Duarte, Sílvia, Vieira, Luis R., Mougeot, François, Vidal, Dolors, Herrera-Rodríguez, Daniel, Rodríguez-Pastor, Ruth, Herrero-Cófreces, Silvia, Jubete, Fernando, Gomes, João Paulo, European Commission, Fundação para a Ciência e a Tecnologia (Portugal), Programa Operacional do Potencial Humano (Portugal), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia e Innovación (España), Isidro, Joana, Escudero, Raquel, Luque-Larena, Juan José, Pinto, Miguel, Borges, Vítor, González Martín-Niño, Rosa, Duarte, Sílvia, Vieira, Luis R., Mougeot, François, Vidal, Dolors, Herrera-Rodríguez, Daniel, Rodríguez-Pastor, Ruth, Herrero-Cófreces, Silvia, Jubete, Fernando, and Gomes, João Paulo
- Abstract
[Introduction] Francisella tularensis is a highly infectious bacterium that causes the zoonotic disease tularemia. The development of genotyping methods, especially those based on whole-genome sequencing (WGS), has recently increased the knowledge on the epidemiology of this disease. However, due to the difficulties associated with the growth and isolation of this fastidious pathogen in culture, the availability of strains and subsequently WGS data is still limited., [Methods] To surpass these constraints, we aimed to implement a culture-free approach to capture and sequence F. tularensis genomes directly from complex samples. Biological samples obtained from 50 common voles and 13 Iberian hares collected in Spain were confirmed as positive for F. tularensis subsp. holarctica and subjected to a WGS target capture and enrichment protocol, using RNA oligonucleotide baits designed to cover F. tularensis genomic diversity., [Results] We obtained full genome sequences of F. tularensis from 13 animals (20.6%), two of which had mixed infections with distinct genotypes, and achieved a higher success rate when compared with culture-dependent WGS (only successful for two animals). The new genomes belonged to different clades commonly identified in Europe (B.49, B.51 and B.262) and subclades. Despite being phylogenetically closely related to other genomes from Spain, the detected clusters were often found in other countries. A comprehensive phylogenetic analysis, integrating 599 F. tularensis subsp. holarctica genomes, showed that most (sub)clades are found in both humans and animals and that closely related strains are found in different, and often geographically distant, countries., [Discussion] Overall, we show that the implemented culture-free WGS methodology yields timely, complete and high-quality genomic data of F. tularensis, being a highly valuable approach to promote and potentiate the genomic surveillance of F. tularensis and ultimately increase the knowledge on the genomics, ecology and epidemiology of this highly infectious pathogen.
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- 2023
8. Microbiological analysis of acute hepatitis of unknown aetiology in children in Spain
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Calvo, Ana Avellon, primary, Munoz-Chimeno, Milagros, additional, Varela, Carmen, additional, Guerrero, Maria, additional, Peñuelas, Marina, additional, Morago, Lucia, additional, Sanchez, Nazaret, additional, Gonzalez, Monica, additional, Tarrago, David, additional, Echevarria, Juan Emilio, additional, Fernandez, Maria Dolores, additional, Escudero, Raquel, additional, Pozo, Francisco, additional, Varona, Sarai, additional, Hernando, Victoria, additional, and Diaz, Asuncion, additional
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- 2023
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9. Dermacentor-borne necrosis erythema lymphadenopathy (DEBONEL) due to Rickettsia raoultii in a patient with erythema migrans
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Norman, Francesca F, primary, Sánchez-Martín, Jesús, additional, Rodríguez-Domínguez, Mario, additional, Escudero, Raquel, additional, and Martín, Oihane, additional
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- 2023
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10. Enteroaggregative Escherichia coli as etiological agent of endemic diarrhea in Spain: A prospective multicenter prevalence study with molecular characterization of isolates
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Llorente, María Teresa, primary, Escudero, Raquel, additional, Ramiro, Raquel, additional, Remacha, María Antonia, additional, Martínez-Ruiz, Rocío, additional, Galán-Sánchez, Fátima, additional, de Frutos, Mónica, additional, Elía, Matilde, additional, Onrubia, Isabel, additional, and Sánchez, Sergio, additional
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- 2023
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11. Density-dependent prevalence of Francisella tularensis in fluctuating vole populations, northwestern Spain
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Rodriguez-Pastor, Ruth, Escudero, Raquel, Vidal, Dolors, Mougeot, Francois, Arroyo, Beatriz, Lambin, Xavier, Vila-Coro, Ave Maria, Rodriguez-Moreno, Isabel, Anda, Pedro, and Luque-Larena, Juan J.
- Subjects
Research ,Risk factors ,Tularemia -- Risk factors -- Research - Abstract
Emerging infectious zoonotic diseases are increasing worldwide, and most zoonoses are linked to wildlife (1,2). Thus, quantifying disease prevalence in potential wildlife hosts is critical to understanding the outbreak dynamics [...]
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- 2017
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12. Reduced susceptibility to biocides in Acinetobacter baumannii: association with resistance to antimicrobials, epidemiological behaviour, biological cost and effect on the expression of genes encoding porins and efflux pumps
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Fernández-Cuenca, Felipe, Tomás, María, Caballero-Moyano, Francisco-Javier, Bou, Germán, Martínez-Martínez, Luis, Vila, Jordi, Pachón, Jerónimo, Cisneros, José-Miguel, Rodríguez-Baño, Jesús, Pascual, Álvaro, Garnacho, José, Gutierrez-Pizarraya, Antonio, Márquez-Vácaro, Juan Antonio, Cano, María Eliecer, Fariñas, M. Carmen, Sánchez-Porto, Antonio, Meruendano, Gloria Esteban, Barbeyto-Vales, Luis, Casas-Ciria, Javier, Vallejo, Luis, Fernández-Pérez, Begona, Villar-Chao, José Carlos, Padilla-Ortega, Belén, Cercenado-Mansilla, Emilia, García-Irure, José Javier, del Arco Jiménez, Alfonso, Gimeno-Cardona, Concepción, Valía, Juan Carlos, Tormo-Palop, Núria, Abril, Vicente, Rifa, Josefina, Martinez-Garcia, Maria Jesus, Vilaró-Pujals, Joseph, Aguirre, Marian Navarro, Vilamala, Ana, Jiménez-Alfaro, José Antonio, Reviejo-Jaca, Carlos, Casanova, Pilar Marín, Guerreo, Francisca, Shaw, Evelyn, Plasencia, Virginia, Nebreda-Mayoral, Teresa, Fernández-Calavia, María José, de Cruz, Susana García, Aldea-Mansilla, Carmen, de Lucas, Esperanza Merino, Zorraquino, Alfredo, Reus-Bañuls, Sergio, Garduno-Eseverri, Eugenio, Sánchez, Luis López, Fleites-Gutiérrez, Ana, Rodríguez-Guardado, Azucena, Moreno, Alfonso, García-Arenzana Anguera, José María, López-Palmero, Serafín, Rodríguez-Maresca, Manuel, García-Garrote, Fernando, Varela-Otero, José, del Pilar Alonso, María, Vidal-Verdú, Elisa, Rodríguez-López, Fernando, Pardo-Sánchez, Fernanda, Ferrer-Vizoso, E., Regueiro-Garcia, B., Gurgui, Mercé, Pericas, Roser, Pomar, Virginia, Olaechea-Astigarraga, Pedro María, Ayarza-Igartua, Rafael, Maciá-Romero, María Dolores, de Gopegui-Bordes, Enrique Ruiz, Sánchez-Romero, María Isabel, García-Mata, Jesús, Goyanes, María José, Morales-Mateos, Cristina, Hernández-Quero, José, Escobar-Lara, Trinidad, Ballester-Bastardie, Frederic, Iftimie, Simona, Pujol-Bajador, Isabel, Galán-Navarro, María Isabel, Cádiz-Gurrea, María Luz, Amores-Antequera, Carmen, Gómez, Montserrat, Cantudo, Purificación, Martí-Salas, Carmina, Cuquet-Peragosa, Jordi, Moreno-Flores, Antonio, Anibarro-García, Luis, Hernando-Real, Susana, Carrero-González, Pablo A., Pallarés-González, María Angeles, Rodríguez-Fernández, Sergio, Pujol-Rojo, Miquel, Tubau, Fe, Nuno-Alvarez, Enrique, Ortega-Torres, María, Giner-Almaraz, Salvador, Roca-Castelló, María Rosa, Castillo, Manuela, Hortelano, Elena, Chaves-Sánchez, Fernando, García-Reyne, Ana, Horcajada-Gallego, Juan Pablo, Segura, Concha, Sierra-Dorado, Gema, Yano-Escudero, Raquel, Dorta-Hung, María Elena, and del Rosario Q, Cristóbal
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- 2015
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13. The importance of lizards and small mammals as reservoirs for Borrelia lusitaniae in Portugal
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Norte, Ana Cláudia, da Silva, António Alves, Alves, Joana, da Silva, Luís Pascoal, Núncio, Sofia M., Escudero, Raquel, Anda, Pedro, Ramos, Jaime A., and de Carvalho, Isabel Lopes
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- 2015
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14. Monotherapy versus combination therapy for sepsis due to multidrug-resistant Acinetobacter baumannii: analysis of a multicentre prospective cohort
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López-Cortés, L. E., Cisneros, J. M., Fernández-Cuenca, F., Bou, G., Tomás, M., Garnacho-Montero, J., Pascual, A., Martínez-Martínez, L., Vila, J., Pachón, J., Rodríguez Baño, J., Porto, Antonio Sánchez, Vallejo, Luis, Pérez, Begoña Fernández, Villar Chao, José Carlos, Ortega, Belén Padilla, Mansilla, Emilia Cercenado, García Irure, José Javier, del Arco Jiménez, Alfonso, de la Torre Lima, Javier, Cardona, Concepción Gimeno, Abril, Vicente, Pujals, Joseph Vilaró, Aguirre, Marian Navarro, Jiménez Alfaro, José Antonio, Jaca, Carlos Reviejo, Casanova, Pilar Marín, Guerreo, Francisca, Mayoral, Teresa Nebreda, Fernández Calavia, María José, de Lucas, Esperanza Merino, Zorraquino, Alfredo, Eseverri, Eugenio Garduño, Sánchez, Luis López, Gutiérrez, Ana Fleites, Guardado, Azucena Rodríguez, García-Arenzana Anguera, José María, Palmero, Serafín López, Maresca, Manuel Rodríguez, Garrote, Fernando García, Otero, José Varela, Verdú, Elisa Vidal, López, Fernando Rodríguez, Sánchez, Fernanda Pardo, Vizoso, E. Ferrer, Gurgui, Mercé, Pericas, Roser, Olaechea Astigarraga, Pedro María, Igartua, Rafael Ayarza, Maciá Romero, María Dolores, de Gopegui Bordes, Enrique Ruiz, Sánchez Romero, María Isabel, Mata, Jesús García, Goyanes, María José, Quero, José Hernández, Lara, Trinidad Escobar, Bastardie, Frederic Ballester, Iftimie, Simona, Galán Navarro, María Isabel, Cádiz Gurrea, María Luz, Antequera, Carmen Amores, Gómez, Montserrat, Salas, Carmina Martí, Peragosa, Jordi Cuquet, Real, Susana Hernando, Carrero González, Pablo A., Pallarés González, María Angeles, Fernández, Sergio Rodríguez, Rojo, Miquel Pujol, Tubau, Fe, Alvarez, Enrique Nuño, Torres, María Ortega, Almaraz, Salvador Giner, Hortelano, Elena, Sánchez, Fernando Chaves, Reyne, Ana García, Horcajada Gallego, Juan Pablo, Segura, Concha, Dorado, Gema Sierra, Escudero, Raquel Yano, Dorta Hung, María Elena, and del Rosario Q, Cristóbal
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- 2014
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15. Characterization of plasmids carrying the blaOXA-24/40 carbapenemase gene and the genes encoding the AbkA/AbkB proteins of a toxin/antitoxin system
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Mosqueda, Noraida, Gato, Eva, Roca, Ignasi, López, María, de Alegría, Carlos Ruíz, Fernández Cuenca, Felipe, Martínez-Martínez, Luis, Pachón, Jerónimo, Cisneros, José Miguel, Rodríguez-Baño, Jesús, Pascual, Álvaro, Vila, Jordi, Bou, Germán, Tomás, María, Garnacho, José, Pizarraya, Antonio Gutierrez, Márquez Vácaro, Juan Antonio, Cano, María Eliecer, Fariñas, M. Carmen, Porto, Antonio Sánchez, Meruendano, Gloria Esteban, Vales, Luis Barbeyto, Ciria, Javier Casas, Vallejo, Luis, Pérez, Begona Fernández, Chao, José Carlos Villar, Ortega, Belén Padilla, Mansilla, Emilia Cercenado, Irure, José Javier García, del Arco Jiménez, Alfonso, Cardona, Concepción Gimeno, Valía, Juan Carlos, Palop, Núria Tormo, Abril, Vicente, Rifa, Josefina, Jesus, Maria, Garcia, Martinez, Pujals, Joseph Vilaró, Aguirre, Marian Navarro, Vilamala, Ana, Alfaro, José Antonio Jiménez, Jaca, Carlos Reviejo, Casanova, Pilar Marín, Guerreo, Francisca, Shaw, Evelyn, Plasencia, Virginia, Mayoral, Teresa Nebreda, Calavia, María José Fernández, de Cruz, Susana García, Mansilla, Carmen Aldea, de Lucas, Esperanza Merino, Zorraquino, Alfredo, Bañuls, Sergio Reus, Eseverri, Eugenio Garduno, Sánchez, Luis López, Gutiérrez, Ana Fleites, Guardado, Azucena Rodríguez, Moreno, Alfonso, Anguera, José María García-Arenzana, Palmero, Serafín López, Maresca, Manuel Rodríguez, Garrote, Fernando García, Otero, José Varela, del Pilar Alonso, María, Verdú, Elisa Vidal, López, Fernando Rodríguez, Sánchez, Fernanda Pardo, Vizoso, E. Ferrer, Garcia, B. Regueiro, Gurgui, Mercé, Pericas, Roser, Pomar, Virginia, Astigarraga, Pedro María Olaechea, Igartua, Rafael Ayarza, Romero, María Dolores Maciá, de Gopegui Bordes, Enrique Ruiz, Romero, María Isabel Sánchez, Mata, Jesús García, Goyanes, María José, Mateos, Cristina Morales, Quero, José Hernández, Lara, Trinidad Escobar, Bastardie, Frederic Ballester, Iftimie, Simona, Bajador, Isabel Pujol, Navarro, María Isabel Galán, Gurrea, María Luz Cádiz, Antequera, Carmen Amores, Gómez, Montserrat, Cantudo, Purificación, Salas, Carmina Martí, Peragosa, Jordi Cuquet, Flores, Antonio Moreno, García, Luis Anibarro, Real, Susana Hernando, González, Pablo A. Carrero, González, María Angeles Pallarés, Fernández, Sergio Rodríguez, Rojo, Miquel Pujol, Tubau, Fe, Alvarez, Enrique Nuno, Torres, María Ortega, Almaraz, Salvador Giner, Castelló, María Rosa Roca, Castillo, Manuela, Hortelano, Elena, Sánchez, Fernando Chaves, Reyne, Ana García, Gallego, Juan Pablo Horcajada, Segura, Concha, Dorado, Gema Sierra, Escudero, Raquel Yano, Hung, María Elena Dorta, and del Rosario Q, Cristóbal
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- 2014
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16. Patterns of Bartonella infection in small mammal from intensive farmlands, NW Spain
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Herrero-Cófreces, Silvia, Escudero, Raquel, Mougeot, François, Castro, N., and Luque-Larena, Juan José
- Abstract
Póster presentado a la Joint Virtual Conference of the WDA and EWDA, celebrada del 31 de agosto al 2 de septiembre de 2021.
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- 2021
17. The roles of mammalian predators in the epidemiology of Francisella tularensis in north west Spain
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Mougeot, François, Escudero, Raquel, Lambin, Xavier, Jubete, Fernando, Vidal, Dolors, Herrero-Cófreces, Silvia, Gonzales, Rosa, Merino, Veronica, Jado, Isabel, and Luque-Larena, Juan José
- Abstract
Resumen del trabajo presentado a la Joint Virtual Conference of the WDA and EWDA, celebrada del 31 de agosto al 2 de septiembre de 2021.
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- 2021
18. Identification of a new Borrelia species among small mammals in areas of northern Spain where Lyme disease is endemic
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Gil, Horacio, Barral, Marta, Escudero, Raquel, Garcia-Perez, Ana L., and Anda, Pedro
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Lyme disease -- Analysis ,Biological sciences - Abstract
The role of small mammals as reservoir hosts for Borrelia burgdorferi is investigated in several areas of northern Spain where Lyme disease is endemic. The results show that although small mammals do not seem to play any major role as reservoirs for B. burgdorferi sensu lato in the northern Spain, they seem to be implicated in the maintenance of spirochetes similar to R57.
- Published
- 2005
19. FRI-153 - Microbiological analysis of acute hepatitis of unknown aetiology in children in Spain
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Calvo, Ana Avellon, Munoz-Chimeno, Milagros, Varela, Carmen, Guerrero, Maria, Peñuelas, Marina, Morago, Lucia, Sanchez, Nazaret, Gonzalez, Monica, Tarrago, David, Echevarria, Juan Emilio, Fernandez, Maria Dolores, Escudero, Raquel, Pozo, Francisco, Varona, Sarai, Hernando, Victoria, and Diaz, Asuncion
- Published
- 2023
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20. Genetic Traces of the Francisella tularensis Colonization of Spain, 1998–2020
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Myrtennäs, Kerstin, primary, Escudero, Raquel, additional, Zaballos, Ángel, additional, González-Martín-Niño, Rosa, additional, Gyuranecz, Miklós, additional, and Johansson, Anders, additional
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- 2020
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21. Genetic Traces of the Francisella tularensis Colonization of Spain, 1998-2020
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Myrtennas, Kerstin, Escudero, Raquel, Zaballos, Angel, Gonzalez-Martin-Nino, Rosa, Gyuranecz, Miklos, Johansson, A., Myrtennas, Kerstin, Escudero, Raquel, Zaballos, Angel, Gonzalez-Martin-Nino, Rosa, Gyuranecz, Miklos, and Johansson, A.
- Abstract
More than 1000 humans have acquired the febrile disease tularemia in Spain since the first notification of human cases in 1997. We here aimed to study the recent molecular evolution of the causative bacterium Francisella tularensis during disease establishment in Spain. Single-nucleotide polymorphisms (SNPs) and variable-number tandem repeats (VNTRs) were analyzed in whole-genome sequences (WGS) of F. tularensis. Short-read WGS data for 20 F. tularensis strains from humans infected in the periods 2014-2015 and 2018-2020 in Spain were generated. These data were combined with WGS data of 25 Spanish strains from 1998 to 2008 and two reference strains. Capillary electrophoresis data of VNTR genetic regions were generated and compared with the WGS data for the 11 strains from 2014 to 2015. Evolutionary relationships among strains were analyzed by phylogenetic methods. We identified 117 informative SNPs in a 1,577,289-nucleotide WGS alignment of 47 F. tularensis genomes. Forty-five strains from Spain formed a star-like SNP phylogeny with six branches emerging from a basal common node. The most recently evolved genomes formed four additional star-like structures that were derived from four branches of the basal common node. VNTR copy number variation was detected in two out of 10 VNTR regions examined. Genetic clustering of strains by VNTRs agreed with the clustering by SNPs. The SNP data provided higher resolution among strains than the VNTRs data in all but one cases. There was an excellent correlation between VNTR marker sizing by capillary electrophoresis and prediction from WGS data. The genetic data strongly support that tularemia, indeed, emerged recently in Spain. Distinct genetic patterns of local F. tularensis population expansions imply that the pathogen has colonized a previously disease-free geographical area. We also found that genome-wide SNPs provide higher genetic resolution among F. tularensis genomes than the use of VNTRs, and that VNTR copy numbers can
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- 2020
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22. ¿Son eficaces los recubrimientos pulpares en dentición temporal?
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Coello Suanzes, José Antonio, Barbero Navarro, Ignacio, Universidad de Sevilla. Departamento de Estomatología, Pastor Escudero, Raquel, Coello Suanzes, José Antonio, Barbero Navarro, Ignacio, Universidad de Sevilla. Departamento de Estomatología, and Pastor Escudero, Raquel
- Abstract
Objetivo: La presente revisión de la literatura tiene como objetivo principal conocer la efectividad de los Recubrimientos pulpares directos e indirectos en dientes primarios, además de conocer las diferentes técnicas de tratamiento, los factores que influyen en la eficacia del mismo y los criterios pronósticos. Material y métodos: Se realizó una búsqueda bibliográfica en las bases de datos Pubmed, FAMA y Cochrane Libary. Aplicando los criterios de inclusión y exclusión, se seleccionaron 10 artículos finalmente para llevar a cabo los resultados y la discusión. Conclusión: Los recubrimientos pulpares en dientes temporales se consideran una técnica correcta, efectiva y reproducible en la clínica. Se necesitan más ensayos y estudios clínicos a largo plazo que demuestren la eficacia de unos medicamentos pulpares frente a otros además de otros factores incluidos en el estudio., Objective: The main objective of this literature review is to know the effectiveness of direct and indirect pulp coatings on primary teeth, in addition to knowing the different treatment techniques, the factors that influence its efficacy and prognostic criteria. Material and methods: A bibliographic search was performed in the Pubmed, FAMA and Cochrane Libary databases. Applying the inclusion and exclusion criteria, 10 articles were finally selected to carry out the results and discussion. Conclusion: Pulp coverings in temporary teeth are considered a correct, effective and reproducible technique in the clinic. More trials and long-term clinical studies are needed to demonstrate the efficacy of some pulp medications against others in addition to other factors included in the study.
- Published
- 2020
23. An unexpected cause of dysphagia: pleural mesothelioma
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Santos-Seoane, Sagrario Maria, Yano-Escudero, Raquel, and Arenas-Garcia, Victor
- Published
- 2019
24. Phylogeography and Genetic Diversity of Francisella tularensis subsp. holarctica in France (1947–2018)
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Kevin, Maëllys, primary, Girault, Guillaume, additional, Caspar, Yvan, additional, Cherfa, Moulay Ali, additional, Mendy, Christiane, additional, Tomaso, Herbert, additional, Gavier-Widen, Dolores, additional, Escudero, Raquel, additional, Maurin, Max, additional, Durand, Benoît, additional, Ponsart, Claire, additional, and Madani, Nora, additional
- Published
- 2020
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25. Rapid and Highly Sensitive DNA Flow Technology Platform to Detect Tick-Borne Bacterial Pathogens in Clinical Samples
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Jado, Isabel, primary, Escudero, Raquel, additional, Espigares, Belén, additional, Lara, Ester, additional, Rodriguez-Vargas, Manuela, additional, Garcia-Amil, Cristina, additional, Lobo, Bruno, additional, Rodriguez-Moreno, Isabel, additional, and Toledo, Alvaro, additional
- Published
- 2020
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26. Molecular method for the characterization of Coxiella burnetii from clinical and environmental samples: variability of genotypes in Spain
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Jado Isabel, Carranza-Rodríguez Cristina, Barandika Jesús, Toledo Álvaro, García-Amil Cristina, Serrano Beatriz, Bolaños Margarita, Gil Horacio, Escudero Raquel, García-Pérez Ana L, Olmeda A, Astobiza Ianire, Lobo Bruno, Rodríguez-Vargas Manuela, Pérez-Arellano José, López-Gatius Fernando, Pascual-Velasco Francisco, Cilla Gustavo, Rodríguez Noé F, and Anda Pedro
- Subjects
Microbiology ,QR1-502 - Abstract
Abstract Background Coxiella burnetii is a highly clonal microorganism which is difficult to culture, requiring BSL3 conditions for its propagation. This leads to a scarce availability of isolates worldwide. On the other hand, published methods of characterization have delineated up to 8 different genomic groups and 36 genotypes. However, all these methodologies, with the exception of one that exhibited limited discriminatory power (3 genotypes), rely on performing between 10 and 20 PCR amplifications or sequencing long fragments of DNA, which make their direct application to clinical samples impracticable and leads to a scarce accessibility of data on the circulation of C. burnetii genotypes. Results To assess the variability of this organism in Spain, we have developed a novel method that consists of a multiplex (8 targets) PCR and hybridization with specific probes that reproduce the previous classification of this organism into 8 genomic groups, and up to 16 genotypes. It allows for a direct characterization from clinical and environmental samples in a single run, which will help in the study of the different genotypes circulating in wild and domestic cycles as well as from sporadic human cases and outbreaks. The method has been validated with reference isolates. A high variability of C. burnetii has been found in Spain among 90 samples tested, detecting 10 different genotypes, being those adaA negative associated with acute Q fever cases presenting as fever of intermediate duration with liver involvement and with chronic cases. Genotypes infecting humans are also found in sheep, goats, rats, wild boar and ticks, and the only genotype found in cattle has never been found among our clinical samples. Conclusions This newly developed methodology has permitted to demonstrate that C. burnetii is highly variable in Spain. With the data presented here, cattle seem not to participate in the transmission of C. burnetii to humans in the samples studied, while sheep, goats, wild boar, rats and ticks share genotypes with the human population.
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- 2012
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27. Rickettsia monacensis and human disease, Spain
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Jado, Isabel, Oteo, Jose A., Aldamiz, Mikel, Gil, Horacio, Escudero, Raquel, Ibarra, Valvanera, Portu, Joseba, Portillo, Aranzazu, Lezaun, Maria J., Garcia-Amil, Cristina, Rodriguez-Moreno, Isabel, and Anda, Pedro
- Subjects
Research ,Health aspects ,Causes of ,Communicable diseases -- Research ,Rickettsiae -- Health aspects -- Research ,Rickettsial diseases -- Causes of -- Research ,Rickettsia -- Health aspects -- Research - Abstract
We identified Rickettsia monacensis as a cause of acute tickborne rickettsiosis in 2 humans. Its pathogenic role was assessed by culture and detection of the organism in patients' blood samples. [...]
- Published
- 2007
28. Arthropod-Borne Bacteria Cause Nonmalarial Fever in Rural Ethiopia: A Cross-Sectional Study in 394 Patients
- Author
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Ramos, Jose M., primary, Pérez-Tanoira, Ramón, additional, Martín-Martín, Inés, additional, Prieto-Pérez, Laura, additional, Tefasmariam, Abraham, additional, Tiziano, Gebre, additional, Escudero, Raquel, additional, Gil-Zamorano, Judit, additional, Gil-Gil, Horacio, additional, Górgolas, Miguel, additional, and Jado, Isabel, additional
- Published
- 2019
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29. Complement factor H binding by different Lyme disease and relapsing fever Borrelia in animals and human
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Gil Horacio, Camafeita Emilio, Escudero Raquel, Bhide Mangesh R, Jado Isabel, and Anda Pedro
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Medicine ,Biology (General) ,QH301-705.5 ,Science (General) ,Q1-390 - Abstract
Abstract Background Borreliae employ multiple immune evasive strategies such as binding to complement regulatory proteins [factor H (fH) and factor H like-1 (FHL1)], differential regulation of surface membrane proteins, antigenic variation, and binding of plasminogen/plasmin and matrix metalloproteinases. As a complement regulatory subunit, fH serves as a cofactor for the factor I-mediated cleavage of C3b. fH binding by Borrelia has been correlated with pathogenesis as well as with host diversity. Here we show the differential binding of borrelial proteins to fH from human and animal sera. Findings Affinity ligand binding experiments, 2-D electrophoresis, and protein identification and peptide de novo sequencing based on mass spectrometry, revealed novel fH putative binding proteins of Lyme- and relapsing fever Borrelia. An OspA serotype-associated differential human and animal fH binding by B. garinii was also observed, which could be related with the ability of some strains from serotypes 4 and 7 to invade non-nervous system tissues. Also, the variable affinity of binding proteins expressed by different Borrelia to animal fH correlated with their host selectivity. Conclusion The novel animal and human putative fH binding proteins (FHBPs) in this study underscore the importance of evasion of complement in the pathogenesis of Borrelia infections.
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- 2009
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30. Zoonotic pathogens in fluctuating common vole (Microtus arvalis) populations: occurrence and dynamics
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Ministerio de Economía y Competitividad (España), Banco Santander, Universidad de Valladolid, Rodríguez-Pastor, Ruth, Escudero, Raquel, Lambin, Xavier, Vidal, Dolors, Gil, Horacio, Jado, Isabel, Rodríguez-Vargas, Manuela, Luque-Larena, Juan José, Mougeot, François, Ministerio de Economía y Competitividad (España), Banco Santander, Universidad de Valladolid, Rodríguez-Pastor, Ruth, Escudero, Raquel, Lambin, Xavier, Vidal, Dolors, Gil, Horacio, Jado, Isabel, Rodríguez-Vargas, Manuela, Luque-Larena, Juan José, and Mougeot, François
- Abstract
Diseases and host dynamics are linked, but their associations may vary in strength, be time-lagged, and depend on environmental influences. Where a vector is involved in disease transmission, its dynamics are an additional influence, and we often lack a general understanding on how diseases, hosts and vectors interact. We report on the occurrence of six zoonotic arthropod-borne pathogens (Anaplasma, Bartonella, Borrelia, Coxiella, Francisella and Rickettsia) in common voles (Microtus arvalis) throughout a population fluctuation and how their prevalence varies according to host density, seasonality and vector prevalence. We detected Francisella tularensis and four species of Bartonella, but not Anaplasma, Borrelia, Coxiella or Rickettsia. Bartonella taylorii and B. grahamii prevalence increased and decreased with current host (vole and mice) density, respectively, and increased with flea prevalence. Bartonella doshiae prevalence decreased with mice density. These three Bartonella species were also more prevalent during winter. Bartonella rochalimae prevalence varied with current and previous vole density (delayed-density dependence), but not with season. Coinfection with F. tularensis and Bartonella occurred as expected from the respective prevalence of each disease in voles. Our results highlight that simultaneously considering pathogen, vector and host dynamics provide a better understanding of the epidemiological dynamics of zoonoses in farmland rodents.
- Published
- 2019
31. Zoonotic bacteria in fleas parasitizing common voles, northwestern Spain
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Ministerio de Economía y Competitividad (España), European Commission, Banco Santander, Universidad de Valladolid, Rodríguez-Pastor, Ruth, Mougeot, François, Vidal, Dolors, Jado, Isabel, González Martín-Niño, Rosa, Escudero, Raquel, Luque-Larena, Juan José, Ministerio de Economía y Competitividad (España), European Commission, Banco Santander, Universidad de Valladolid, Rodríguez-Pastor, Ruth, Mougeot, François, Vidal, Dolors, Jado, Isabel, González Martín-Niño, Rosa, Escudero, Raquel, and Luque-Larena, Juan José
- Abstract
We detected Francisella tularensis and Bartonella spp. in fleas parasitizing common voles (Microtus arvalis) from northwestern Spain; mean prevalence was 6.1% for F. tularensis and 51% for Bartonella spp. Contrasted vector–host associations in the prevalence of these bacteria suggest that fleas have distinct roles in the transmission cycle of each pathogen in nature.
- Published
- 2019
32. Fractura craneal evolutiva. Extensión intradiploica occipital del quiste leptomeníngeo
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Escudero, Raquel, Pérez, Isabel, Roldán, Florinda, and Canabal, Ana
- Published
- 2001
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33. Genome-scale comparison of Francisella tularensis strains isolated in an endemic region of Spain
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Lopes, Isabel, Pinto, Miguel, Rodríguez-Pastor, Ruth, Isidro, Joana, Nunes, Carolina, Mougeot, François, Vidal, Dolors, Luque-Larena, Juan José, and Escudero, Raquel
- Abstract
Resumen del trabajo presentado a la 9th International Conference on Tularemia, celebrada en Montréal (Canada) del 16 al 19 de octubre de 2018., [Background and aim]: Tularemia is caused by the gram-negative intracellular pathogen Francisella tularensis. In Europe, the region of Castilla y León, Northwest Spain, is a major hotspot for tularemia, where the largest outbreaks of the disease have been recently reported. While rodents and lagomorphs are recognised as the main mammalian hosts in Europe, the common voles (Microtus arvalis) are documented key agents for human tularemia in Northwestern Spain, as evidenced by a spatial and temporal coincidence between human cases and increases in vole abundance. This study aimed to perform comparative genomics of F. tularensis isolates from tissue samples of F. tularensis positive voles with 11 human isolates from 2014, when an increased number of human cases was observed in the same area of Spain. [Material and Methods]: For this study, we selected thirty-four trapped voles, sampled in 80 km2 of farmland in Palencia Province, Spain (42°1′N, 4°42′W), that tested Ft-positive, by conventional PCR and hybridization by reverse line blotting (targeting lpnA), and the multi-target TaqMan PCR, tul4 and ISFtu2 assays. Tissues (liver, spleen and lung) from each animal were minced, inoculated in chocolate agar PolyViteXTM at 37°C in 5% CO2, and observed at 24, 48 and 72h post-inoculation. So far, after DNA extraction, four isolates, showing typical Ft growth, were subjected to paired-end whole-genome sequencing in an Illumina MiSeq apparatus, followed by genome assembly and bioinformatics analysis. [Results and conclusions]: All trapped voles tested positive for F. tularensis subsp. holarctica. A preliminary core-genome SNPs-based analysis, representing >99% of the genome, showed that all isolates (N=15) were distinguishable by 33 single nucleotide variant sites, representing two clear phylogenetic clusters. Noteworthy, four newly sequenced vole-isolated strains segregated together with five human-associated strains from 2014, suggesting the potential persistence of this strain in this region.
- Published
- 2018
34. Molecular evidence of Francisella tularensis in nature, 10 years after a large outbreak of tularemia in Spain
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Vidal, Dolors, Mougeot, François, Rodríguez-Pastor, Ruth, Jubete, Fernando, Arroyo, Beatriz, González Martín-Niño, Rosa, Jado, Isabel, Luque-Larena, Juan José, and Escudero, Raquel
- Subjects
bacterial infections and mycoses - Abstract
Resumen del trabajo presentado a la 9th International Conference on Tularemia, celebrada en Montréal (Canada) del 16 al 19 de octubre de 2018., [Background and aim]: Tularemia is a zoonosis caused by Francisella tularensis, a pathogen that can use a variety of transmission routes in nature as well as in humans. Since 1997 more than 1,000 human cases of tularemia have been declared in Spain in two major outbreaks, but knowledge about the epidemiology of this infection is very limited. The ability of F. tularensis to find mechanisms to persist in the environment and cause new outbreaks makes it necessary to identify, among others, the potential reservoirs during inter-epizootic periods, the main objective of this study. [Material and methods]: The sampling points were located in an area of Castilla y León, well known as “hot spots” for tularemia. They corresponded to surface water and sediments, as well as crayfish, mosquitoes, hares and ticks. Specifically, 8 zones divided into 4 subzones were sampled, including three rivers, three irrigation channels and three lagunes. Samples were collected in July and November of 2016 and 2017. For detection of Francisella lpnA was amplified by conventional PCR and hybridization by reverse line blotting with specific probes. Positive samples were confirmed by a real-time multitarget TaqMan PCR, using tul4 and ISFtu2 assays. For additional characterization sdhA gen and VNTR Ft-M19 were amplified and sequenced. [Results]: A total of 102 samples of water, 111 sediments, 343 crayfish, 156 mosquitos, 64 hares and 126 ticks from 14 hares were collected. Overall, 2 samples of water, 3 of sediments, 7 hares and 17 ticks tested positive for Francisella. Interestingly, F. tularensis subspecie holarctica was detected in water, hares and ticks while F. hispaniensis was the species detected in water and sediments. [Conclusions]: In this study we provide evidence of Francisella presence in nature during periods in which no large outbreaks of tularemia occur, and suggest that hares and ticks play an important role in the maintenance of the bacteria in the terrestrial environment. Moreover, to the best of our knowledge this is the first description of F. hispaniensis in abiotic environment, which enforces the need to assess the risk for the population and establish the necessary measures for its prevention and control.
- Published
- 2018
35. Zoonotic pathogens in fluctuating common vole (Microtus arvalis) populations: occurrence and dynamics
- Author
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Rodríguez-Pastor, Ruth, primary, Escudero, Raquel, additional, Lambin, Xavier, additional, Vidal, Mª Dolors, additional, Gil, Horacio, additional, Jado, Isabel, additional, Rodríguez-Vargas, Manuela, additional, Luque-Larena, Juan José, additional, and Mougeot, François, additional
- Published
- 2018
- Full Text
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36. Instalación eléctrica de un edificio destinado a viviendas
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Collantes Escudero, Raquel, Rodríguez Matilla, María del Pilar, and Universidad de Valladolid. Escuela de Ingenierías Industriales
- Subjects
Instalaciones eléctricas domésticas - Abstract
El presente trabajo de fin de grado es el proyecto de la instalación eléctrica de baja tensión de un edificio de viviendas cumpliendo la normativa vigente, a destacar el CTE y el REBT. El edificio del que es objeto este proyecto consta de tres plantas y un sótano. Está formado por 10 viviendas, 4 de dos dormitorios y 6 de tres dormitorios, ascensor, 2 locales comerciales y en el sótano 10 trasteros y garaje adaptado a la nueva instrucción técnica complementaria 52 del reglamento electrotécnico de baja tensión: ''Infraestructura para la Recarga de Vehículos Eléctricos'', que entró en vigor en Julio de 2015. Para la elaboración de este proyecto como herramientas de software han sido utilizados en especial los programas AutoCAD 2016 y CYPE, modulo CYPECAD MEP 2016., Departamento de Ingeniería Eléctrica, Grado en Ingeniería Eléctrica
- Published
- 2016
37. Francisella tularensis in a wild population of common vole (Microtus arvalis) from agricultural landscapes of SW Europe
- Author
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Rodríguez-Pastor, Ruth, Escudero, Raquel, Vidal, Dolors, Mougeot, François, Arroyo, Beatriz, Vila-Coro, Ave Maria, Rodríguez-Moreno, Isabel, Anda, Pedro, Lambin, Xavier, and Luque-Larena, Juan José
- Abstract
Resumen del trabajo presentado al 15th Rodens et Spatium: International Conference on Rodent Biology, celebrada en Olomouc (Czech Republic) del 25 al 29 de julio de 2016., In many European regions, most microtine rodents exhibit multi-annual fluctuations in abundance and can cause significant crop damage and economical losses when they are overabundant during population outbreaks. In addition, they can be reservoirs of multiple pathogens responsible of serious zoonotic diseases to humans and livestock. Therefore, understanding the relationships between rodents and their pathogens under a dynamic perspective (density-dependent patterns of prevalence) may be particularly relevant in terms of effective public health management. In NW Spain, the outbreaking common vole (Microtus arvalis) recently colonized intensive agricultural landscapes where it has been pointed as a main amplification and spill-over host of Francisella tularensis (the etiological agent of tularemia). However, the epidemiological role of these fluctuating vole populations with regard to zoonotic diseases remains largely unknown. Here we describe for the first time the patterns of prevalence of F. tularensis along a complete fluctuation in abundance in a wild common vole population. Voles were captured seasonally (March, July and November) during a population outbreak between 2013 and 2015 and were screened for the occurrence of the pathogen. From a total of 243 captured voles, 46 individuals were infected, but prevalence greatly varied in time (range from 0 to 30%).We found a strong effect of vole abundance on tularemia prevalence, in a direct positive density-dependent way. We discuss the implications of the findings for our understanding of vole population regulation and public health. The increasing frequency of zoonotic disease events in rodents encourage us to go on studying the co-infection in common vole populations with other relevant pathogens.
- Published
- 2016
38. Análisis del sector vitivinícola en Francia=Analysis of French wine sector
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Castro Escudero, Raquel, López González, Hermenegildo, Algebra, and Facultad de Ciencias Economicas y Empresariales
- Subjects
Francia ,Sector vitivinícola ,Comercio exterior ,Análisis ,Vino ,Importación ,Comercio ,Empresas ,Exportación ,Economía - Abstract
En el presente trabajo se lleva a cabo un análisis de la situación actual del sector vitivinícola en Francia. Francia se encuentra entre los líderes en este sector a nivel mundial, siendo líder en cuanto a nivel de producción, en superficie destinada al viñedo, en consumo y en volumen y valor de sus exportaciones. En primer lugar, se establecen el conjunto de factores que influyen en el sector del vino en Francia. Estos factores son determinantes en nuestro análisis puesto que se trata de las características que definen la situación actual del sector. Entre estos factores destaca la producción, la estructura empresarial, el consumo, el precio o los canales de distribución utilizados en el mercado del vino en Francia. En segundo lugar, se ha realizado un análisis del comercio exterior en Francia, estableciendo primero la situación de los indicadores económicos fundamentales en el análisis del comercio internacional y posteriormente analizando las exportaciones e importaciones desarrolladas en Francia en el año 2015. Dentro de las mismas hemos establecido una división que diferencia entre categoría de producto y país de destino o procedencia. Dentro de dicha división, de nuevo establecemos una distinción entre volumen y valor de las exportaciones e importaciones. Posteriormente, como síntesis del estudio realizado, se han detallado una serie de perspectivas del mercado del vino en Francia. Para ello se ha desarrollado un escueto análisis de la coyuntura internacional para establecer más correctamente la situación del mercado vitivinícola francés a nivel mundial. Finalmente, se extraen una serie de conclusiones fruto del análisis desarrollado
- Published
- 2016
39. Irruptive mammal host populations shape tularemia epidemiology
- Author
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Luque-Larena, Juan J., primary, Mougeot, François, additional, Arroyo, Beatriz, additional, Vidal, Mª Dolors, additional, Rodríguez-Pastor, Ruth, additional, Escudero, Raquel, additional, Anda, Pedro, additional, and Lambin, Xavier, additional
- Published
- 2017
- Full Text
- View/download PDF
40. Density-dependent prevalence of Francisella tularensis in fluctuating vole populations, northwestern Spain
- Author
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European Commission, Banco Santander, Universidad de Valladolid, Ministerio de Economía y Competitividad (España), Rodríguez-Pastor, Ruth, Escudero, Raquel, Vidal, Dolors, Mougeot, François, Arroyo, Beatriz, Lambin, Xavier, Vila-Coro, Ave Maria, Rodríguez-Moreno, Isabel, Anda, Pedro, Luque-Larena, Juan José, European Commission, Banco Santander, Universidad de Valladolid, Ministerio de Economía y Competitividad (España), Rodríguez-Pastor, Ruth, Escudero, Raquel, Vidal, Dolors, Mougeot, François, Arroyo, Beatriz, Lambin, Xavier, Vila-Coro, Ave Maria, Rodríguez-Moreno, Isabel, Anda, Pedro, and Luque-Larena, Juan José
- Abstract
Tularemia in humans in northwestern Spain is associated with increases in vole populations. Prevalence of infection with Francisellatularensis in common voles increased to 33% during a vole population fluctuation. This finding confirms that voles are spillover agents for zoonotic outbreaks. Ecologic interactions associated with tularemia prevention should be considered.
- Published
- 2017
41. Irruptive mammal host populations shape tularemia epidemiology
- Author
-
Ministerio de Economía y Competitividad (España), Luque-Larena, Juan José, Mougeot, François, Arroyo, Beatriz, Vidal, Dolors, Rodríguez-Pastor, Ruth, Escudero, Raquel, Anda, Pedro, Lambin, Xavier, Ministerio de Economía y Competitividad (España), Luque-Larena, Juan José, Mougeot, François, Arroyo, Beatriz, Vidal, Dolors, Rodríguez-Pastor, Ruth, Escudero, Raquel, Anda, Pedro, and Lambin, Xavier
- Abstract
Pearls: Host population dynamics are the key of wildlife zoonotic risk; Aquatic and terrestrial agents of tularemia coexist in nature; Irrigation has provided aquatic reservoirs and a grass-loving amplification agent for tularemia in Spain; Fluctuating mammalian populations shape tularemia epidemiology; Tularemia surveillance must target unstable mammalian host populations.
- Published
- 2017
42. Long-range dispersal moved Francisella tularensis into Western Europe from the East
- Author
-
Dwibedi, Chinmay Kumar, Birdsell, Dawn, Larkeryd, Adrian, Myrtennas, Kerstin, Ohrman, Caroline, Nilsson, Elin, Karlsson, Edvin, Hochhalter, Christian, Rivera, Andrew, Maltinsky, Sara, Bayer, Brittany, Keim, Paul, Scholz, Holger C., Tomaso, Herbert, Wittwer, Matthias, Beuret, Christian, Schuerch, Nadia, Pilo, Paola, Hernandez Perez, Marta, Rodriguez-Lazaro, David, Escudero, Raquel, Anda, Pedro, Forsman, Mats, Wagner, David M., Larsson, Par, Johansson, Anders, Dwibedi, Chinmay Kumar, Birdsell, Dawn, Larkeryd, Adrian, Myrtennas, Kerstin, Ohrman, Caroline, Nilsson, Elin, Karlsson, Edvin, Hochhalter, Christian, Rivera, Andrew, Maltinsky, Sara, Bayer, Brittany, Keim, Paul, Scholz, Holger C., Tomaso, Herbert, Wittwer, Matthias, Beuret, Christian, Schuerch, Nadia, Pilo, Paola, Hernandez Perez, Marta, Rodriguez-Lazaro, David, Escudero, Raquel, Anda, Pedro, Forsman, Mats, Wagner, David M., Larsson, Par, and Johansson, Anders
- Abstract
For many infections transmitting to humans from reservoirs in nature, disease dispersal patterns over space and time are largely unknown. Here, a reversed genomics approach helped us understand disease dispersal and yielded insight into evolution and biological properties of Francisella tularensis, the bacterium causing tularemia. We whole-genome sequenced 67 strains and characterized by single-nucleotide polymorphism assays 138 strains, collected from individuals infected 1947-2012 across Western Europe. We used the data for phylogenetic, population genetic and geographical network analyses. All strains (n= 205) belonged to a monophyletic population of recent ancestry not found outside Western Europe. Most strains (n= 195) throughout the study area were assigned to a star-like phylogenetic pattern indicating that colonization of Western Europe occurred via clonal expansion. In the East of the study area, strains were more diverse, consistent with a founder population spreading from east to west. The relationship of genetic and geographic distance within the F. tularensis population was complex and indicated multiple long-distance dispersal events. Mutation rate estimates based on year of isolation indicated null rates; in outbreak hotspots only, there was a rate of 0.4 mutations/genome/year. Patterns of nucleotide substitution showed marked AT mutational bias suggestive of genetic drift. These results demonstrate that tularemia has moved from east to west in Europe and that F. tularensis has a biology characterized by long-range geographical dispersal events and mostly slow, but variable, replication rates. The results indicate that mutation-driven evolution, a resting survival phase, genetic drift and long-distance geographical dispersal events have interacted to generate genetic diversity within this species.
- Published
- 2016
- Full Text
- View/download PDF
43. Análisis del sector vitivinícola en Francia=Analysis of French wine sector
- Author
-
López González, Hermenegildo, Algebra, Castro Escudero, Raquel, López González, Hermenegildo, Algebra, and Castro Escudero, Raquel
- Abstract
En el presente trabajo se lleva a cabo un análisis de la situación actual del sector vitivinícola en Francia. Francia se encuentra entre los líderes en este sector a nivel mundial, siendo líder en cuanto a nivel de producción, en superficie destinada al viñedo, en consumo y en volumen y valor de sus exportaciones. En primer lugar, se establecen el conjunto de factores que influyen en el sector del vino en Francia. Estos factores son determinantes en nuestro análisis puesto que se trata de las características que definen la situación actual del sector. Entre estos factores destaca la producción, la estructura empresarial, el consumo, el precio o los canales de distribución utilizados en el mercado del vino en Francia. En segundo lugar, se ha realizado un análisis del comercio exterior en Francia, estableciendo primero la situación de los indicadores económicos fundamentales en el análisis del comercio internacional y posteriormente analizando las exportaciones e importaciones desarrolladas en Francia en el año 2015. Dentro de las mismas hemos establecido una división que diferencia entre categoría de producto y país de destino o procedencia. Dentro de dicha división, de nuevo establecemos una distinción entre volumen y valor de las exportaciones e importaciones. Posteriormente, como síntesis del estudio realizado, se han detallado una serie de perspectivas del mercado del vino en Francia. Para ello se ha desarrollado un escueto análisis de la coyuntura internacional para establecer más correctamente la situación del mercado vitivinícola francés a nivel mundial. Finalmente, se extraen una serie de conclusiones fruto del análisis desarrollado
- Published
- 2016
44. Long-range dispersal moved Francisella tularensis into Western Europe from the East
- Author
-
Dwibedi, Chinmay, primary, Birdsell, Dawn, additional, Lärkeryd, Adrian, additional, Myrtennäs, Kerstin, additional, Öhrman, Caroline, additional, Nilsson, Elin, additional, Karlsson, Edvin, additional, Hochhalter, Christian, additional, Rivera, Andrew, additional, Maltinsky, Sara, additional, Bayer, Brittany, additional, Keim, Paul, additional, Scholz, Holger C., additional, Tomaso, Herbert, additional, Wittwer, Matthias, additional, Beuret, Christian, additional, Schuerch, Nadia, additional, Pilo, Paola, additional, Hernández Pérez, Marta, additional, Rodriguez-Lazaro, David, additional, Escudero, Raquel, additional, Anda, Pedro, additional, Forsman, Mats, additional, Wagner, David M., additional, Larsson, Pär, additional, and Johansson, Anders, additional
- Published
- 2016
- Full Text
- View/download PDF
45. Zoonotic pathogens in fluctuating common vole (Microtus arvalis) populations: occurrence and dynamics.
- Author
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Rodríguez-Pastor, Ruth, Escudero, Raquel, Lambin, Xavier, Vidal, Mª Dolors, Gil, Horacio, Jado, Isabel, Rodríguez-Vargas, Manuela, Luque-Larena, Juan José, and Mougeot, François
- Subjects
- *
ZOONOSES , *MICROTUS arvalis , *DISEASE vectors , *ANAPLASMA , *BARTONELLA - Abstract
Diseases and host dynamics are linked, but their associations may vary in strength, be time-lagged, and depend on environmental influences. Where a vector is involved in disease transmission, its dynamics are an additional influence, and we often lack a general understanding on how diseases, hosts and vectors interact. We report on the occurrence of six zoonotic arthropod-borne pathogens (Anaplasma , Bartonella , Borrelia , Coxiella , Francisella and Rickettsia) in common voles (Microtus arvalis) throughout a population fluctuation and how their prevalence varies according to host density, seasonality and vector prevalence. We detected Francisella tularensis and four species of Bartonella , but not Anaplasma , Borrelia , Coxiella or Rickettsia. Bartonella taylorii and B. grahamii prevalence increased and decreased with current host (vole and mice) density, respectively, and increased with flea prevalence. Bartonella doshiae prevalence decreased with mice density. These three Bartonella species were also more prevalent during winter. Bartonella rochalimae prevalence varied with current and previous vole density (delayed-density dependence), but not with season. Coinfection with F. tularensis and Bartonella occurred as expected from the respective prevalence of each disease in voles. Our results highlight that simultaneously considering pathogen, vector and host dynamics provide a better understanding of the epidemiological dynamics of zoonoses in farmland rodents. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
46. Ureteral Iatrogenic. Findings at multidetector computed tomography
- Author
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Escudero, Raquel
- Subjects
Catheters ,Abdomen ,Pathology ,Urinary Tract / Bladder ,CT - Abstract
Purpose Methods and Materials Results Conclusion References Personal Information, Purpose: The Multidetector Computed Tomography(MDCT) is very useful given the importance of characterizing the type and extent of ureteral injury, location (it can be upper, middle or bottom location), time of diagnosis (immediate or deferred) and the...
- Published
- 2012
- Full Text
- View/download PDF
47. Francisella tularensis, Portugal
- Author
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de Carvalho, Isabel Lopes, Escudero, Raquel, Garcia-Amil, Cristina, Falcao, Helena, Anda, Pedro, and Nuncio, Maria Sofia
- Subjects
Prevention ,Research ,Causes of ,Disease transmission -- Research -- Prevention ,Tularemia -- Causes of -- Prevention -- Research - Abstract
To the Editor: Tularemia is a zoonosis caused by Francisella tularensis. Recently, tularemia has emerged in new locations, populations, and settings (1). After an outbreak in Spain in 1997 (2), [...]
- Published
- 2007
48. Tularemia Outbreaks and Common Vole (Microtus arvalis) Irruptive Population Dynamics in Northwestern Spain, 1997–2014
- Author
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Luque-Larena, Juan José, primary, Mougeot, François, additional, Roig, Dolors Vidal, additional, Lambin, Xavier, additional, Rodríguez-Pastor, Ruth, additional, Rodríguez-Valín, Elena, additional, Anda, Pedro, additional, and Escudero, Raquel, additional
- Published
- 2015
- Full Text
- View/download PDF
49. The importance of lizards and small mammals as reservoirs forBorrelia lusitaniaein Portugal
- Author
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Norte, Ana Cláudia, primary, Alves da Silva, António, additional, Alves, Joana, additional, da Silva, Luís Pascoal, additional, Núncio, M. Sofia, additional, Escudero, Raquel, additional, Anda, Pedro, additional, Ramos, Jaime A., additional, and Lopes de Carvalho, Isabel, additional
- Published
- 2015
- Full Text
- View/download PDF
50. Distribution of Bartonella henselae Variants in Patients, Reservoir Hosts and Vectors in Spain
- Author
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Gil, Horacio, primary, Escudero, Raquel, additional, Pons, Inmaculada, additional, Rodríguez-Vargas, Manuela, additional, García-Esteban, Coral, additional, Rodríguez-Moreno, Isabel, additional, García-Amil, Cristina, additional, Lobo, Bruno, additional, Valcárcel, Félix, additional, Pérez, Azucena, additional, Jiménez, Santos, additional, Jado, Isabel, additional, Juste, Ramón, additional, Segura, Ferrán, additional, and Anda, Pedro, additional
- Published
- 2013
- Full Text
- View/download PDF
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