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2. Prävalenz und Charakterisierung azolresistenter Aspergillus fumigatus-Isolate bei deutschen Mukoviszidose-Patienten

Author

Seufert, R, Dittmer, S, Killengray, D, Schmidt, D, Eskandarian, N, Buer, J, Rath, PM, Steinmann, J, and ARAF Study Group

Subjects
ddc: 610, 610 Medical sciences, Medicine
Abstract

Zielsetzung:Aspergillus fumigatus ist häufigste aus Atemwegen isolierte Fadenpilz bei Patienten mit Cystischer Fibrose (Mukoviszidose, CF) [ref:1]. Ziel dieser prospektiven multizentrischen Studie war es, die Prävalenz von azolresistenten A. fumigatus-Stämmen (ARAF) in Atemwegssekreten[zum vollständigen Text gelangen Sie über die oben angegebene URL], Infektiologie Update 2018; 26. Jahrestagung der Paul-Ehrlich-Gesellschaft für Chemotherapie (PEG)

Published
2018
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3. Prevalence and characterization of azole-resistant Aspergillus fumigatus in patients with cystic fibrosis: a prospective multicentre study in Germany

Author

Seufert, R., Sedlacek, L., Kahl, B., Hogardt, M., Hamprecht, A., Haase, G., Gunzer, F., Haas, A., Grauling-Halama, S., MacKenzie, C. R., Essig, A., Stehling, F., Sutharsan, S., Dittmer, S., Killengray, D., Schmidt, D., Eskandarian, N., Steinmann, E., Buer, J., Hagen, F., Meis, J. F., Rath, P. -M., Steinmann, J., Seufert, R., Sedlacek, L., Kahl, B., Hogardt, M., Hamprecht, A., Haase, G., Gunzer, F., Haas, A., Grauling-Halama, S., MacKenzie, C. R., Essig, A., Stehling, F., Sutharsan, S., Dittmer, S., Killengray, D., Schmidt, D., Eskandarian, N., Steinmann, E., Buer, J., Hagen, F., Meis, J. F., Rath, P. -M., and Steinmann, J.

Abstract

Objectives: Aspergillus fumigatus is the most prevalent filamentous fungus in the respiratory tract of patients with cystic fibrosis (CF). The aim of this prospective multicentre study was to investigate the prevalence of azole-resistant A. fumigatus (ARAF) in respiratory secretions from CF patients across Germany and to characterize ARAF isolates by phenotypic and molecular methods. Methods: Twelve tertiary care centres from Germany participated in the study. In total, 2888 A. fumigatus isolates from 961 CF patients were screened for ARAF by using azole-containing agar plates. Antifungal susceptibility testing of isolates was performed by broth microdilution according to EUCAST guidelines. Analysis of mutations mediating resistance was performed using PCR and sequencing of the cyp51A gene. Furthermore, genotyping by microsatellite PCR was performed. Results: Of a total of 2888 A. fumigatus isolates, 101 isolates from 51 CF patients were found to be azole resistant (prevalence per patient 5.3%). The Essen centre had the highest prevalence (9.1%) followed by Munich (7.8%), Munster (6.0%) and Hannover (5.2%). Most ARAF isolates (n = 89) carried the TR34/L98H mutation followed by eight G54E/R, one TR46/Y121F/T289A and one F219S mutation. In two isolates no mutation was found. Genotyping results showed no major clustering. Forty-five percent of CF patients with ARAF had previously received azole therapy. Conclusions: This is the first multicentre study analysing the prevalence of ARAF isolates in German CF patients. Because of a resistance rate of up to 9%, susceptibility testing of A. fumigatus isolates from CF patients receiving antifungal treatment should be part of standard diagnostic work-up.

Published
2018

4. Prevalence and characterization of azole-resistant Aspergillus fumigatus in patients with cystic fibrosis: a prospective multicentre study in Germany

Author

Seufert, R, Sedlacek, L, Kahl, B, Hogardt, M, Hamprecht, A, Haase, G, Gunzer, F, Haas, A, Grauling-Halama, S, MacKenzie, C R, Essig, A, Stehling, F, Sutharsan, S, Dittmer, S, Killengray, D, Schmidt, D, Eskandarian, N, Steinmann, E, Buer, J, Hagen, F, Meis, J F, Rath, P-M, Steinmann, J, Seufert, R, Sedlacek, L, Kahl, B, Hogardt, M, Hamprecht, A, Haase, G, Gunzer, F, Haas, A, Grauling-Halama, S, MacKenzie, C R, Essig, A, Stehling, F, Sutharsan, S, Dittmer, S, Killengray, D, Schmidt, D, Eskandarian, N, Steinmann, E, Buer, J, Hagen, F, Meis, J F, Rath, P-M, and Steinmann, J

Abstract

Objectives: Aspergillus fumigatus is the most prevalent filamentous fungus in the respiratory tract of patients with cystic fibrosis (CF). The aim of this prospective multicentre study was to investigate the prevalence of azole-resistant A. fumigatus (ARAF) in respiratory secretions from CF patients across Germany and to characterize ARAF isolates by phenotypic and molecular methods.Methods: Twelve tertiary care centres from Germany participated in the study. In total, 2888 A. fumigatus isolates from 961 CF patients were screened for ARAF by using azole-containing agar plates. Antifungal susceptibility testing of isolates was performed by broth microdilution according to EUCAST guidelines. Analysis of mutations mediating resistance was performed using PCR and sequencing of the cyp51A gene. Furthermore, genotyping by microsatellite PCR was performed.Results: Of a total of 2888 A. fumigatus isolates, 101 isolates from 51 CF patients were found to be azole resistant (prevalence per patient 5.3%). The Essen centre had the highest prevalence (9.1%) followed by Munich (7.8%), Münster (6.0%) and Hannover (5.2%). Most ARAF isolates (n = 89) carried the TR34/L98H mutation followed by eight G54E/R, one TR46/Y121F/T289A and one F219S mutation. In two isolates no mutation was found. Genotyping results showed no major clustering. Forty-five percent of CF patients with ARAF had previously received azole therapy.Conclusions: This is the first multicentre study analysing the prevalence of ARAF isolates in German CF patients. Because of a resistance rate of up to 9%, susceptibility testing of A. fumigatus isolates from CF patients receiving antifungal treatment should be part of standard diagnostic work-up.

Published
2018

5. Prevalence and characterization of azole-resistant Aspergillus fumigatus in patients with cystic fibrosis: a prospective multicentre study in Germany

Author

Seufert, R, primary, Sedlacek, L, additional, Kahl, B, additional, Hogardt, M, additional, Hamprecht, A, additional, Haase, G, additional, Gunzer, F, additional, Haas, A, additional, Grauling-Halama, S, additional, MacKenzie, C R, additional, Essig, A, additional, Stehling, F, additional, Sutharsan, S, additional, Dittmer, S, additional, Killengray, D, additional, Schmidt, D, additional, Eskandarian, N, additional, Steinmann, E, additional, Buer, J, additional, Hagen, F, additional, Meis, J F, additional, Rath, P -M, additional, and Steinmann, J, additional

Published
2018
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8. Characterization of a Group B Streptococcus infection based on the demographics, serotypes, antimicrobial susceptibility and genotypes of selected isolates from sterile and non-sterile isolation sites in three major hospitals in Malaysia.

Author

Suhaimi MES, Desa MNM, Eskandarian N, Pillay SG, Ismail Z, Neela VK, Masri SN, and Nordin SA

Subjects
Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Demography, Diabetes Complications, Female, Genetic Variation, Hospitals, Humans, Immunocompromised Host, Infant, Infant, Newborn, Malaysia epidemiology, Male, Microbial Sensitivity Tests, Middle Aged, Random Amplified Polymorphic DNA Technique, Risk Factors, Streptococcal Infections epidemiology, Streptococcal Infections pathology, Streptococcus agalactiae drug effects, Young Adult, Drug Resistance, Bacterial, Genotype, Serogroup, Streptococcal Infections microbiology, Streptococcus agalactiae classification, Streptococcus agalactiae isolation & purification
Abstract

Background/purpose: The purpose of this study is to characterize GBS isolates that were collected from three major hospitals in a densely populated area of Klang Valley for their demographics, serotypes, antibiotic susceptibility patterns and genetic background., Methods: Sixty GBS isolates from sterile and non-sterile samples in three major hospitals in the Klang Valley area of Malaysia were collected by convenience sampling from 2012 until March 2014. These isolates were studied for their antimicrobial susceptibilities, serotypes and genotypes. Patients' demographic data and clinical information were collected from lab request forms., Results: Diabetes mellitus was the only underlying condition (7 patients, 23.3%); the remaining samples were from patients who were immunocompromised due to medications. Fifty-nine (98%) isolates were sensitive to penicillin, while 78.3% and 88.3% of the isolates were sensitive to erythromycin and clindamycin, respectively. Serotype Ia was the most common serotype (n=27, 45%), followed by serotype III (n=10, 16.7%), V (n=9, 15%), VI (n=8, 13.3%), VIII (n=2, 3.3%) and VII (n=1, 1.7%). Random Amplified Polymorphic DNA (RAPD) typing showed a diverse genetic pedigree for all isolates, including four major groups that clustered according to geographical location., Conclusion: This preliminary study determines the prevalence of limited common serotypes and antimicrobial resistance in distinct GBS isolates. Nonetheless, the RAPD clustering pattern suggests a close genetic lineage of the GBS isolates based on their isolation sites and location of hospitals., (Copyright © 2016 King Saud Bin Abdulaziz University for Health Sciences. Published by Elsevier Ltd. All rights reserved.)

Published
2017
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9. Genetic Analysis of the Atrial Natriuretic Peptide Gene Polymorphisms among Essential Hypertensive Patients in Malaysia.

Author

Ghodsian N, Ismail P, Ahmadloo S, Eskandarian N, and Etemad A

Subjects
Aged, Diabetes Mellitus, Type 2 pathology, Essential Hypertension, Female, Genetic Association Studies, Genotype, Humans, Hypertension pathology, Malaysia, Male, Middle Aged, Polymorphism, Single Nucleotide, Atrial Natriuretic Factor genetics, Diabetes Mellitus, Type 2 genetics, Genetic Predisposition to Disease, Hypertension genetics
Abstract

Background. Atrial natriuretic peptide (ANP) considerably influences blood pressure regulation through water and sodium homoeostasis. Several of the studies have utilized anonymous genetic polymorphic markers and made inconsequent claims about the ANP relevant disorders. Thus, we screened Insertion/Deletion (ID) and G191A polymorphisms of ANP to discover sequence variations with potential functional significance and to specify the linkage disequilibrium pattern between polymorphisms. The relationships of detected polymorphisms with EH with or without Type 2 Diabetes Mellitus (T2DM) status were tested subsequently. Method. ANP gene polymorphisms (I/D and A191G) were specified utilizing mutagenically separated Polymerase Chain Reaction (PCR) in 320 subjects including 163 EH case subjects and 157 controls. Result. This case-control study discovered a significant association between I/D polymorphisms of ANP gene in EH patient without T2DM. However, the study determined no association between G191A polymorphisms of ANP in EH with or without T2DM. In addition, sociodemographic factors in the case and healthy subjects exhibited strong differences (P < 0.05). Conclusion. As a risk factor, ANP gene polymorphisms may affect hypertension. Despite the small sample size in this study, it is the first research assessing the ANP gene polymorphisms in both EH and T2DM patients among Malaysian population.

Published
2016
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