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17 results on '"Eta, Runa"'

8. Peptidoglycan-Like Components in Z-100, Extracted from Strain Aoyama B, Increase IL-12p40 via NOD2.

Author

Horii, Takayuki, Orikawa, Yuki, Ohira, Yuta, Eta, Runa, Tominaga, Uiko, Sato, Takanori, and Tanaka, Takao

Subjects
MYCOBACTERIUM tuberculosis, GRANULOCYTE-macrophage colony-stimulating factor, PRODUCTION increases, POLYSACCHARIDES, ANIMAL experimentation, CELL receptors, TUMOR necrosis factors, LIPIDS, MICE
Abstract

Background: Z-100 is a hot-water extract of the human-type Mycobacterium tuberculosis strain Aoyama B. While Z-100's macrophage-mediated immunomodulatory effects have been reported, the mechanistic details have not been fully clarified. Here, we studied the immunomodulatory effects of Z-100 on mouse bone marrow-derived cells, human CD14+ cells, and skin.Methods: Mouse bone marrow-derived cells and CD14+ cells were cultured in the presence of granulocyte-macrophage colony-stimulating factor, differentiated into macrophage-like cells, and then stimulated with Z-100. Furthermore, since Z-100 is subcutaneously administered clinically, we injected Z-100 into mice and measured gene expression in the skin.Results: While Z-100 stimulation increased the production of interleukin- (IL-) 12p40 and IL-1β in mouse bone marrow-derived macrophages, levels of IL-1β were low. In contrast, TNF-α production did not increase. Meanwhile, stimulation of human CD14+ cells with Z-100 increased production of IL-12p40, TNF-α, and IL-1β. Because Z-100 appeared to have the most stable effect on IL-12p40, we examined the components of Z-100 that induce IL-12p40 production. We found that Z-100 contained peptidoglycan-like components. In addition, an siRNA study showed that Z-100 increased the production of IL-12p40 via nucleotide-binding oligomerization domain 2 (NOD2). Further, subcutaneous administration of Z-100 to mice significantly elevated expression of IL-12p40 and IL-1β and showed a trend towards increasing TNF-α in the skin.Conclusion: Z-100 induced the production of immunomodulatory cytokines from various types of macrophages and specifically increased IL-12p40 production through peptidoglycan-like components via NOD2. [ABSTRACT FROM AUTHOR]

Published
2022
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10. Z-100, an Immunomodulatory Extract of Mycobacterium tuberculosis Strain Aoyama B, Prevents Spontaneous Lymphatic Metastasis of B16-BL6 Melanoma

Author

Horii, Takayuki, primary, Yoshinaga, Koji, additional, Kobayashi, Nobuyoshi, additional, Seto, Koichi, additional, Orikawa, Yuki, additional, Okamoto, Masahiro, additional, Eta, Runa, additional, Ohira, Yuta, additional, Katsunuma, Kokichi, additional, Hori, Yuko, additional, Tanaka, Takao, additional, and Takei, Mineo, additional

Published
2014
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12. Acotiamide Hydrochloride (Z-338), a New Selective Acetylcholinesterase Inhibitor, Enhances Gastric Motility without Prolonging QT Interval in Dogs: Comparison with Cisapride, Itopride, and Mosapride

Author

Matsunaga, Yugo, primary, Tanaka, Takao, additional, Yoshinaga, Koji, additional, Ueki, Shigeru, additional, Hori, Yuko, additional, Eta, Runa, additional, Kawabata, Yoshihiro, additional, Yoshii, Kazuyoshi, additional, Yoshida, Kenji, additional, Matsumura, Toshihiro, additional, Furuta, Shigeru, additional, Takei, Mineo, additional, Tack, Jan, additional, and Itoh, Zen, additional

Published
2010
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13. Pharmacological Evaluation of Analgesic Effects of the Cholecystokinin2 Receptor Antagonist Z-360 in Mouse Models of Formalin- and Cancer-Induced Pain

Author

Yoshinaga, Koji, primary, Horii, Takayuki, additional, Hamano, Hiroki, additional, Eta, Runa, additional, Ozaki, Tomoko, additional, Orikawa, Yuki, additional, Yoshii, Kazuyoshi, additional, Kawabata, Yoshihiro, additional, Hori, Yuko, additional, Seto, Koichi, additional, Takei, Mineo, additional, and Kuraishi, Yasushi, additional

Published
2010
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17. Acotiamide Hydrochloride (Z-338), a New Selective Acetylcholinesterase Inhibitor, Enhances Gastric Motility without Prolonging QT Interval in Dogs: Comparison with Cisapride, Itopride, and Mosapride.

Author

Matsunaga, Yugo, Tanaka, Takao, Yoshinaga, Koji, Ueki, Shigeru, Hori, Yuko, Eta, Runa, Kawabata, Yoshihiro, Yoshii, Kazuyoshi, Yoshida, Kenji, Matsumura, Toshihiro, Furuta, Shigeru, Takei, Mineo, Tack, Jan, and Itoh, Zen

Abstract

Acotiamide hydrochloride (acotiamide; N-[2-[bis(1-methylethyl) amino]ethyl]-2-[(2-hydroxy-4,5-dimethoxybenzoyl) amino] thiazole-4-carboxamide monohydrochloride trihydrate, Z-338) has been reported to improve meal-related symptoms of functional dyspepsia in clinical studies. Here, we examined the gastroprokinetic effects of acotiamide and its antiacetylcholinesterase activity as a possible mechanism of action in conscious dogs. Acotiamide increased postprandial gastric motor activity in conscious dogs with chronically implanted force transducers and, like itopride, mosapride, and cisapride, exhibited gastroprokinetic activity in these dogs. Furthermore, acotiamide improved clonidine-induced hypomotility and delayed gastric emptying. Acotiamide-enhanced postprandial gastroduodenal motility was suppressed completely by pretreatment with atropine, a muscarinic receptor antagonist. In in vitro studies, acotiamide enhanced acetylcholine- but not carbachol-induced contractile responses of guinea pig gastric antrum strips. Moreover, like itopride and neostigmine, acotiamide inhibited recombinant human and canine stomach-derived acetylcholinesterase (AChE) activity in vitro. The mode of the AChE inhibitory action of acotiamide was selective and reversible. Unlike itopride or mosapride, acotiamide showed no affinity for dopamine D(2) or serotonin 5-HT(4) receptors. With regard to cardiovascular side effects, unlike cisapride, acotiamide did not affect myocardial monophasic action potential duration, QT interval, or corrected QT interval in anesthetized dogs. These results suggest that acotiamide stimulates gastric motility in vivo by inhibiting AChE activity without affecting QT interval. Acotiamide thus represents a beneficial new drug for the treatment of functional dyspepsia involving gastric motility dysfunction, with differences from other prokinetic agents.

Published
2011
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