1. Photochemotherapy induces the apoptosis of monocytes without impairing their function
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Françoise Gabert, Joel Plumas, Jean-Paul Molens, Dalil Hannani, Olivier Hequet, David Laurin, Laurence Chaperot, Mariam Sall, Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), EFS Rhône-Alpes, EFS, INSERM U823, équipe 9 (Immunobiologie et Immunothérapie des Cancers), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS Rhône-Alpes, and Etablissement Français du Sang et Association Recherche et Transfusion.
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MESH: Cell Death ,medicine.medical_treatment ,Cell Culture Techniques ,MESH: PUVA Therapy ,MESH: Chemotaxis, Leukocyte ,Apoptosis ,Adaptive Immunity ,MESH: Monocytes ,Monocytes ,chemistry.chemical_compound ,0302 clinical medicine ,Photopheresis ,Immunophenotyping ,Cell Movement ,Reference Values ,MESH: Cell Movement ,Psoralen ,Cells, Cultured ,0303 health sciences ,Cell Death ,MESH: Dendritic Cells ,Extracorporeal Photochemotherapy ,MESH: Reference Values ,Cell Differentiation ,Acquired immune system ,Chemotaxis, Leukocyte ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,PUVA therapy ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,MESH: Photopheresis ,MESH: Cells, Cultured ,MESH: Cell Differentiation ,Programmed cell death ,MESH: Immunophenotyping ,Biology ,Article ,Proinflammatory cytokine ,03 medical and health sciences ,medicine ,Humans ,PUVA Therapy ,030304 developmental biology ,Immunobiology ,MESH: Photochemotherapy ,Transplantation ,MESH: Cell Culture Techniques ,MESH: Humans ,Monocyte ,MESH: Apoptosis ,Dendritic Cells ,chemistry ,Photochemotherapy ,MESH: Monocytes, Activated Killer ,Immunology ,Monocytes, Activated Killer ,MESH: Adaptive Immunity - Abstract
International audience; BACKGROUND: Extracorporeal photopheresis (ECP) is a powerful therapy currently used to treat various hematological disorders as in graft versus host disease. Clinical data clearly demonstrate its efficacy and immunomodulation toward the pathogenic T cells. However, ECP mechanism of action is still poorly understood. Monocytes represent up to 30% of the total amount of treated cells and are known to play an important role in adaptive immunity. However, data from previous reports analyzing the effect of psoralen and UV-A irradiation (PUVA) on their functions are heterogeneous. In this study, we focused on the effect of PUVA on human monocytes functions in adaptive immunity. DESIGN AND METHODS: Purified human monocytes were treated in vitro by PUVA. We measured their kinetic of apoptosis after the treatment. We also determine whether their phenotype and functionalities were modified. Finally, we assessed the functionalities of PUVA-treated monocytes-derived dendritic cells (DC). RESULTS: PUVA treatment sentenced purified monocytes to die in 6 days and immediately altered their migratory capacities without impairing their ability of endocytosis. It also up-regulated co-stimulatory molecules and production of inflammatory cytokines on activation and consequently stimulated allogeneic or autologous T cells as efficiently as untreated monocytes. Moreover, PUVA-treated monocytes retained their ability to differentiate into fully functional DC that maturated and stimulated T cells as well as normal DC. CONCLUSIONS: Our data demonstrate that monocytes undergo apoptosis and loose a part of their migratory capacity after ECP and the surviving cell functionalities are not impaired, suggesting that monocytes have a minor effect on ECP-mediated immunomodulation.
- Published
- 2010
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