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A substitution of FBS in culturing of bovine satellite cells for the purpose of culturing meat is presented, addressing both basal media and growth factors in relation to proliferating and differentiating cells.

Modern agriculture has responded to the growing pressure for animal-protein consumption in the global human population by selecting for specific production traits, which, over the last fifty years, has resulted in a loss of genetic diversity. Most rare and endangered breeds of livestock have not experienced the same selection pressures for production and therefore may contain useful genetic traits not found within modern breeds. In an effort to maintain biodiversity of livestock breeds, the SVF Foundation, a non-profit organization founded to preserve the genetic diversity of food and fiber livestock, has established an ex situ repository of genetic material from endangered North American cattle, sheep, and goats. This genetic material includes in vivo and in vitro produced embryos, semen, fibroblasts, serum, and whole blood DNA cards. The majority of samples in the SVF repository are cryopreserved, creating a genome resource bank for future use. Through the Smithsonian and SVF Biodiversity Preservation Project, this repository will be maintained at the Smithsonian’s Front Royal, VA, facility. This effort represents an excellent model for understanding and sustaining the genetic diversity of rare breeds in the US and in other countries.