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Introduction: Helicobacter pylori infection is a major issue worldwide, with widespread prevalence, combined with its link to gastritis, peptic ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. Meanwhile, effectiveness of current treatment protocols is limited by increasing antibiotic resistance and patient compliance issues due to long regimens and side effects. Plukenetia volubilis , or sacha inchi, is a valuable source of bioactive molecules. However, studies on its antimicrobial activity, especially against H. pylori , are lacking. Methods: In this study, the anti- H. pylori activity of P. volubilis leaves water extract was explored using in vitro and in silico approaches. High-Performance Liquid Chromatography coupled to Electrospray Ionisation and Quadrupole Time-of-Flight Mass Spectrometry (HPLC-ESI- QTOF-MS-MS) analysis of the water extract from the leaves was used to characterise the chemical composition of the plant and allowed identification of some flavonoids, such as astragalin, and some phenolic compounds. Then, high-speed counter current chromatography (HSCCC) was used to fractionate the ethyl acetate partition obtained from the water extract from the leaves. Results and Discussion: The presence of flavonoids derived from kaempferol was confirmed and astragalin was isolated for the first time in P. volubilis. The P. volubilis water infusion, ethyl acetate extract and the isolated astragalin exhibited anti-bacterial activity against H. pylori J99 and two clinical isolates (e.g., minimum inhibitory concentrations of 0.53, 0.51 and 0.49 μg/mL, respectively, for clarithromycin-resistant clinical isolate SSR366). Then, using molecular docking for potential protein targets for H. pylori , it was verified that astragalin could interact with these proteins by in silico analysis. Conclusion: These findings highlight that P. volubilis and astragalin produce a bacteriostatic activity against H. pylori and may have potential to be used in treatment against H. pylori , after further research. [ABSTRACT FROM AUTHOR]

Plants use extrafloral nectaries (EFNs) as indirect defence mechanisms against herbivores. These structures contain nectar that is offered to ants in exchange for their protection. EFNs display pronounced functional variation, but it is unknown how this variation comes together in phenotypes. Here, we characterized the main functional traits of EFNs and investigated the associations among them. This work was conducted at a study site in the Caatinga that hosts 14 species of woody plants. We characterized the following functional traits: EFN type, position, arrangement, size, and reducing-sugar level. We observed a marked degree of trait variation (~30%) that was manifested in species-specific trait combinations, giving rise to an 'ecological spectrum'. At one end were Fabaceae species with large, vascularized EFNs that occur individually on the leaf petiole and/or rachis and that produce high levels of reducing sugars. At the other end were Euphorbiaceae species with small, nonvascularized EFNs that are generally grouped on the leaf blade and that produce low levels of reducing sugars. Despite its limited geographical and phylogenetic scale, this study represents an important first step in describing an ecological spectrum that can inform our understanding of the ecological interactions and evolutionary history of this functionally relevant group of plants. [ABSTRACT FROM AUTHOR]

Croton palustris (≡ Caperonia palustris) and Croton castaneifolius (≡ Caperonia castaneifolia) were published by Linnaeus in the first edition of Species plantarum. Both names remain untypified at present. While syntypes are extant for C. palustris there is no known original material for C. castaneifolius. Here, a lectotype is selected for C. palustris and a neotype is selected for C. castaneifolius. [ABSTRACT FROM AUTHOR]

The phytochemical investigation of the leaves and twigs of Croton yunnanensis resulted in the isolation of eight new clerodane furanoditerpenoids, named croyunfuranoids A−H (1 − 8), along with three known analogs (9 − 11). The structures of these compounds were elucidated using spectroscopic analyses, and their absolute configurations were determined through a combination of electronic circular dichroism (ECD) calculations and single-crystal X-ray diffraction. Notably, Croyunfuranoid D (4) is identified as a rare 18,19- dinor -clerodane diterpenoid. Additionally, the structure of a previously reported diterpenoid, crotonyunnan B, was revised. All isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages. Among them, compounds 5 and 6 demonstrated significant inhibitory effects, with IC 50 values of 20.33 ± 2.31 and 22.80 ± 1.31 μmol·L−1, respectively. [ABSTRACT FROM AUTHOR]

Psoriasis, an immune-mediated inflammatory skin disorder, seriously affects the quality of life of nearly four percent of the world population. Euphorbia helioscopia L. is the monarch constituent of Chinese ZeQi powder preparation for psoriasis, so it is necessary to illustrate its active ingredients. Thus, twenty-three diterpenoids, including seven new ones, were isolated from the whole herb of E. helioscopia L. Compounds 1 and 2, each featuring a 2,3-dicarboxylic functionality, are the first examples in the ent-2,3-sceo-atisane or the ent-2,3-sceo-abietane family. Extensive spectroscopic analysis (1D, 2D NMR, and HRMS data) and computational methods were used to confirm their structures and absolute configurations. According to the previous study and NMR data from the jatropha diterpenes obtained in this study, some efficient 1H NMR spectroscopic rules for assigning the relative configurations of 3α-benzyloxy-jatroph-11E-ene and 7,8-seco-3α-benzyloxy-jatropha-11E-ene were summarized. Moreover, the hyperproliferation of T cells and keratinocytes is considered a key pathophysiology of psoriasis. Anti-proliferative activities against induced T/B lymphocytes and HaCaT cells were tested, and IC50 values of some compounds ranged from 6.7 to 31.5 μM. Compounds 7 and 11 reduced the secretions of IFN-γ and IL-2 significantly. Further immunofluorescence experiments and a docking study with NF-κB P65 showed that compound 13 interfered with the proliferation of HaCaT cells by inhibiting the NF-κB P65 phosphorylation at the protein level. [ABSTRACT FROM AUTHOR]

Floral diversity of Croton, the second largest genus in Euphorbiaceae, is currently under-explored. Several clades demonstrate an unusual floral morphology, e.g., lower or higher stamen number, bilateral symmetry and reduced ovary, but have never been investigated in a comparative study with typical Croton. This study examined morphology and ontogeny of flowers in nine Croton species from different clades within the genus with light and scanning microscopy, resin sectioning and micro-computed tomography. In staminate flowers, great variations of stamen number and arrangement are observed. The ancestral androecium likely consisted of two or more whorls with the outermost antepetalous stamen whorl developing centrifugally. Modification by reduction of the antepetalous whorl resulted in an outer alternipetalous stamen whorl in Croton section Moacroton, subgenus Quadrilobi. Several species in the subgenus Geiseleria show an independent reduction of stamen numbers by absence of a centrifugal development with the antepetalous whorl the first whorl to develop. Petal losses are observed in the distantly related C. setiger and C. dioicus. Chaotic stamen arrangement is found in C. celtidifolius (subgenus Adenophylli) as a result of a secondary stamen increase. In pistillate flowers, reduction of carpel numbers happened three times in the subgenus Geiseleria. C. monanthogynus has a bicarpellate ovary, while in C. setiger and C. michauxii the ovary is monocarpellate. Reduction of carpel number is linked with merism change and perianth reduction. The ovary in C. michauxii has basal placentation which is unique among all Croton. Moreover, strong bilateral sepals and nectaries are observed in species from section Julocroton. Therefore, the floral diversity of some species in the genus Croton could be explained by developmental modification of an ancestral form via reduction, rearrangement of stamen whorls, and symmetry shifts. [ABSTRACT FROM AUTHOR]

The aim of the present study is to evaluate the potent molluscicidal activity of aqueous stem bark extracts of plant Euphorbia tirucalli (Family: Euphorbiaceae). Lymnaea acuminata were exposed for 24 hours to sublethal doses of crude aqueous stem bark extracts of Euphorbia tirucalli, i.e. 95.67 µM and 191.35 µM (40% and 80% of 24h LC50 of L. acuminata) respectively, and then were switched to extract-free water to determine the effects of withdrawal from treatment. For the following seven days, this water was replaced every 24 hours. Following that, biochemical parameters were assessed in various snail tissues which showed significant (P < 0.05) alteration in the carbohydrates and nitrogenous metabolisms in time and dose dependent manner. The snail tissues recovered in part after 7days completely after withdrawl of toxic aqueous extracts. The aqueous extracts of stem bark of Euphorbia tirucalli may be used as a potent source of molluscicides; being less expensive, easily available, easily soluble in water. [ABSTRACT FROM AUTHOR]

Objectives: The phytochemicals in the aerial parts of Euphorbia paralias (also known as Sea Spurge) and their anti-inflammatory and antimicrobial activities were investigated.Methods: The methanolic extract was characterized using GC-MS and HPLC techniques. The anti-inflammatory feature was estimated through a Human Red Blood Cell (HRBC) membrane stabilization technique, while the antimicrobial feature was evaluated by the disc diffusion agar technique, minimum bactericidal concentration, and minimum inhibitory concentration (MIC) via micro-broth dilution method.Results: The GC/MS results demonstrated the existence of various phytochemicals, such as n-hexadecenoic acid, cis-11-eicosenoic acid, and methyl stearate, recognized for their anti-inflammatory and antibacterial features. The similarity of the phytochemical composition with other Euphorbia species emphasizes the genus-wide similarity. The antiinflammatory activity exhibited a noteworthy inhibitory effect comparable to the reference drug indomethacin. The extract’s antimicrobial potential was tested against a range of microorganisms, demonstrating significant action against Gram-positive bacteria and Candida albicans . The quantification of total phenolics and flavonoids further supported the therapeutic potential of the extract.Conclusion: The methanolic extract from E. paralias emerges as a successful natural source of important active constituents with potential applications as anti-inflammatory and antimicrobial agents. This research provides a first step to valorize Euphorbia paralias insights as a source of worthwhile phytochemicals that have potential applications in the pharmaceutical industry.

The methanolic extract of the leaves of Macaranga hurifolia Beille showed arginase inhibitory activity (40% at 100 µg/mL) and was then fractionated to obtain nine polyphenolic compounds. Their structures were elucidated on the basis of NMR spectroscopic data, and by comparison with data previously reported in the literature, as gallic acid (1), 3,4-dihydroxybenzoic acid (2), chlorogenic acid, (3), corilagin (4), cynaroside (5), cosmosiin (6), hyperoside (7) isoquercitrin (8) and guajaverin (9). These compounds have been evaluated as arginase inhibitors. Compounds 4, 7, 8 and 9 showed varying arginase inhibitory activities with IC50 values ranging from 102 to 302 μM. All the isolated compounds were previously identified in this species but their activities on arginase are reported here for the first time. [ABSTRACT FROM AUTHOR]

Four new norditerpenoid heterodimers with different dimerization patterns—namely, trigofragiloids A–C (denoted as compounds 1–3) and (+)- and (−)-trigofragiloid D (compound 4)—and three new phenanthrenone norditerpenoids—namely, trigofragiloids E–G (compounds 5–7)—were isolated from Trigonostemon fragilis. Compounds 1 and 2 feature a novel heterodimeric carbon skeleton formed by the conjugation of a tetra-norditerpenoid and an ennea-norditerpenoid; they have been identified as class 2 atropisomers by means of quantum chemical calculations. Compound 3 is an unprecedented phenylpropanoid–norditerpenoid adduct with a new dimerization pattern. Compounds (+)- and (−)-4 are the first example of S-shaped 1,4-dioxane-fused norditerpenoid dimers. Inspired by the structure elucidation of compound 4, two co-occurring analogues, actephilol A and epiactephilol A, were structurally revised as a pair of geometrical isomers and were identified as two pairs of enantiomers, (+)- and (−)-8 and (+)- and (−)-9, respectively. Their structures were characterized using a combined method. Notably, compound 7 exhibits remarkable adenosine triphosphate-citrate lyase (ACLY) inhibition with a half-maximal inhibition concentration (IC50) value of (0.46 ± 0.11) μmol∙L−1, as active as the positive control BMS-303141, and a molecular docking study offers deep insight into the interaction between compound 7 and ACLY.

Abstract Chrozophora sabulosa Kar. & Kir. is a biennial herbaceous plant that belongs to the Euphorbiaceae family and has medicinal properties. This research aimed to identify the genetic characteristics and phylogenetic position of the Chrozophora genus within the Euphorbiaceae family. The evolutionary position of the Chrozophora genus was previously unknown due to insufficient research. Therefore, to determine the evolutionary link between C. sabulosa and other related species, we conducted a study using the NGS Illumina platform to sequence the C. sabulosa chloroplast (cp.) genome. The study results showed that the genome was 156,488 bp in length. It had a quadripartite structure consisting of two inverted repeats (IRb and IRa) of 24,649-bp, separated by an 87,696-bp LSC region and a 19,494-bp SSC region. The CP genome contained 113 unique genes, including four rRNA genes, 30 tRNA genes, and 79 CDS genes. In the second copy of the inverted repeat, there were 18 duplicated genes. The C. sabulosa lacks the petD, petB, rpl2, and rps16 intron. The analysis of simple sequence repeats (SSRs) revealed 93 SSR loci of 22 types and 78 oligonucleotide repeats of four kinds. The phylogenetic investigation showed that the Chrozophora genus evolved paraphyletically from other members of the Euphorbiaceae family. To support the phylogenetic findings, we selected species from the Euphorbiaceae and Phyllanthaceae families to compare with C. sabulosa for Ks and Ka substitution rates, InDels investigation, IR contraction and expansion, and SNPs analysis. The results of these comparative studies align with the phylogenetic findings. We identified six highly polymorphic regions shared by both families, which could be used as molecular identifiers for the Chrozophora genus (rpl33-rps18, rps18-rpl20, rps15-ycf1, ndhG-ndhI, psaI-ycf4, petA-psbJ). The cp. genome sequence of C. sabulosa reveals the evolution of plastid sequences in Chrozophora species. This is the first time the cp. genome of a Chrozophora genus has been sequenced, serving as a foundation for future sequencing of other species within the Chrozophoreae tribe and facilitating in-depth taxonomic research. The results of this research will also aid in identifying new Chrozophora species.

Nine jatrophane diterpenoids were isolated from the whole plant Euphorbia helioscopia, including two new ones, helioscopnins A (1) and B (2). Comprehensive spectroscopic data analysis and ECD calculations elucidated their structures, including absolute configurations. All compounds were evaluated for bioactivity towards autophagic flux by flow cytometry using HM mCherry-GFP-LC3 cells. Compounds 1, 3, 4, 5, 8, and 9 significantly increased autophagic flux. [ABSTRACT FROM AUTHOR]

Acalypha godesffiana is a plant used in conventional medicine for fungal-related illnesses. The plant‘s extracts were investigated in this study, antioxidant, and antimicrobial studies were conducted. Different models were employed in the antioxidant assay; serial dilution was utilized to determine the minimum inhibitory concentration (MIC). The extract from A. godseffiana was purified and characterized by chromatographic and spectroscopic techniques, respectively. Three biologically active compounds, 3, 5-dihdroxylbenzoic acid (1), 3, 4, 5-trihydroxybenzoic acid (2), as well as Di-(2-ethylhexyl) phthalate (3), were identified for the first time in A. godseffiana. The extract and fractions exhibited varying scavenging capacities on different anti-oxidative models. The DPPH of MeOH (IC50= 0.51 mg mL−1) was comparable with silymarine (SLY) IC50= 0.52 mg mL−1 and better than gallic acid (GAL) IC50=1.95 mg mL−1; the ABTS.+ of EtOAc column fraction (ACF, IC50=0.46 mg mL−1) was comparable with standard SLY, IC50 = 0.47 mg mL−1; and an OH radical of DCM, IC50= 0.10 mg mL−1, was better than both standards (SLY, IC50= 6.30 mg mL−1, GAL: IC50= 1.93 mg mL−1). ACF showed superior antifungal activities (0.02 mg mL−1) against Cryptococcus neoformans and Candida albicans, compared to ketoconazole (MIC of 0.250 mg mL−1). Compounds (1-3) from A. godseffina reportedly displayed antioxidant and other activities. This study validated the antifungal potentials of A. godseffiana leaves and identified bioactive compounds. The extracts should be further investigated, and the compounds should be added to the existing library for further investigation of possible leads. [ABSTRACT FROM AUTHOR]

Crotonyanes A (1) and B (2), two new cembranoids were isolated from the leaves and twigs of Croton yanhuii Y. T. Chang. Their structures were elucidated by extensive spectroscopic analyses and quantum chemical calculations. Bioassays verified that compound 1 exhibited an inhibitory effect on nitric oxide (NO) production in RAW 264.7 cells, with an IC50 value of 30.6 ± 4.3 µM. [ABSTRACT FROM AUTHOR]

The nomenclatural significance of Thomas Martyn's monumental revision (and expansion) of Philip Miller's Gardeners dictionary, namely Martyn's puzzlingly neglected The gardener's and botanist's dictionary ([1795-]1807), is assessed with particular reference to New World botany. The publication dates of its constituent parts are discussed; a key resource is internal evidence, citation of recently published works, notably Roxburgh's Plants of the coast of Coromandel, publication dates of which are also made more precise through examination of Royal Society of London records. Besides making more secure currently accepted names in Lagerstroemia (Lythraceae), Liatris (Compositae) and Meniscium (Thelypteridaceae), a number of other additions and corrections to current databases are identified, those affecting currently accepted names being disposed of here. This results merely in a new combination in Ctenodon (Leguminosae) being proposed, though Croton cochinchinensis Martyn may well be an earlier name for C. cascarilloides Raeusch. (Euphorbiaceae), while Mimosa carthagenensis Martyn (Leguminosae) is lectotypified such that it conclusively falls into the synonymy of Piptadenia retusa (Jacq.) P.J. Ribeiro & al. (Leguminosae). [ABSTRACT FROM AUTHOR]

The genus Euphorbia (Euphorbiaceae) has near-cosmopolitan distribution and serves as a significant resource for both ornamental and medicinal purposes. Despite its economic importance, Euphorbia's taxonomy has long been challenged by the intricate nature of morphological traits exhibiting high levels of convergence. While molecular markers are essential for phylogenetic studies, their availability for Euphorbia has been limited. To address this gap, we conducted comparative analyses focusing on the chloroplast (CP) genomes of nine Euphorbia species, incorporating three newly sequenced and annotated accessions. In addition, phylogenetic informativeness and nucleotide diversity were computed to identify candidate markers for phylogenetic analyses among closely related taxa in the genus. Our investigation revealed relatively conserved sizes and structures of CP genomes across the studied species, with notable interspecific variations observed primarily in non-coding regions and IR/SC borders. By leveraging phylogenetic informativeness and nucleotide diversity, we identified rpoB gene as the optimal candidate for species delimitation and shallow-level phylogenetic inference within the genus. Through this comprehensive analysis of CP genomes across multiple taxa, our study sheds light on the evolutionary dynamics and taxonomic intricacies of Euphorbia, offering valuable insights into its CP genome evolution and taxonomy. [ABSTRACT FROM AUTHOR]

• Euphorbia schoenlandii has been heavily impacted by habitat loss and degradation. • There is a high degree of isolation between the three remaining populations. • Plants are negatively affected by the stem-boring larvae of nephopteryx divisella. • Extant populations are stable and recruiting overall, especially at coastal sites. Many threatened plant species in South Africa occur outside of protected areas and these include numerous succulent species that are traded internationally. Despite the potential impact that this may have on rare and endemic succulent species, limited population data exist to inform the non-detriment findings required by the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) to ensure that international trade is sustainable. A concerted effort is being made in South Africa to gather such data for threatened succulents in the Euphorbiaceae. As part of this effort, the study set out to locate the extant populations of Euphorbia schoenlandii , an endemic of the Succulent Karoo, and acquire a better understanding of the population biology and ecology of the species. We applied the point-centred quarter method to estimate population size and density at each locality. This first-ever comprehensive field survey allowed for the determination of the geographic distribution of the species, as well as the sizes and number of populations. Biotic and abiotic environmental variables were employed to generate a habitat profile and a species distribution model for conservation purposes. Population structure, regeneration potential, and stability of the three known populations were also assessed. According to this study, approximately 29 152 individuals remain in the wild. It was shown that the species is often associated with nurse plants. Various potential pollinators were identified, but of concern was the considerable infestation by an alien snout moth (Nephopteryx divisella) , a known pest of euphorbias. The first adult size class contained the highest proportion of individuals, which is indicative of pulse recruitment. Seedling numbers were low, most likely because the surveys were conducted during a drought period. Overall, this study provides the first data on various aspects of the population structure and ecology of E. schoenlandii. It provides valuable information regarding current threats, and also highlights the vulnerability of the species to further habitat loss and degradation or future illegal or unregulated harvesting. [ABSTRACT FROM AUTHOR]

A new species of Euphorbia that has been historically treated as E. dentata in Argentina is described and illustrated. The new species, Euphorbia subilsiae, is morphologically similar to E. pentadactyla, the only other native species of the “Euphorbia dentata alliance” in Argentina, but it has bifid styles and less brightly colored umbel bracts. Euphorbia davidii, long thought to be native in Córdoba Province, should be considered as recently introduced in Argentina. It is now a common weed across Buenos Aires Province but is apparently less common further north in the country. A discussion of the distinctions among these species and their taxonomic history in Argentina is presented. [ABSTRACT FROM AUTHOR]

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Euphorbia, one of the largest genera of flowering plants, is well-known for containing many biofuel crops. Euphorbia tirucalli, an evergreen succulent mainly native to the Africa continent but cultivated worldwide, is a promising petroleum plant with high tolerance to drought and salt stress. However, the exploration of such an important plant resource is severely hampered by the lack of a reference genome. Here, we present the chromosome-level genome assembly of E. tirucalli using PacBio HiFi sequencing and Hi-C technology. Its genome size was approximately 745.62 Mb, with a contig N50 of 74.16 Mb. A total of 743.63 Mb (99.73%) of the assembled sequences were anchored to 10 chromosomes with a complete BUSCO score of 97.80%. Genome annotation revealed 26,304 protein-coding genes, and 76.37% of the genome was identified as repeat elements. The high-quality genome provides valuable genetic resources that would be useful for unraveling the genetic mechanisms of biofuel synthesis and evolutionary adaptation of E. tirucalli. [ABSTRACT FROM AUTHOR]

Flowering is the change from the vegetative state to the reproductive state. Flowering initiation is regulated by endogenous genetic components and by numerous environmental factors. These stimuli determine the different molecular mechanisms that are required to give way to flowering and vary according to the dose of the stimulus and the crop used. Jatropha curcas is a perennial shrub that produces oil from its seeds of adequate quality to be transformed into biofuels. However, this plant still presents limitations for its establishment as a commercial crop since it produces low seeds, which can be attributed to its low production of female flowers. This plant also presents multiple and asynchronous flowering periods as a consequence of the accession and of the climatic conditions, which implies that the fruits do not ripen synchronously, and therefore the harvests are constant, manual, and expensive. Knowing the mechanisms that regulate the flowering of J. curcas is important to improve its seed production. Therefore, current revision covers the analysis of genes involved in flowering to gain information on the pathways involved in floral transition and in the differentiation of the sexual organs of J. curcas. [ABSTRACT FROM AUTHOR]

IntroductionHelicobacter pylori infection is a major issue worldwide, with widespread prevalence, combined with its link to gastritis, peptic ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. Meanwhile, effectiveness of current treatment protocols is limited by increasing antibiotic resistance and patient compliance issues due to long regimens and side effects. Plukenetia volubilis, or sacha inchi, is a valuable source of bioactive molecules. However, studies on its antimicrobial activity, especially against H. pylori, are lacking.MethodsIn this study, the anti-H. pylori activity of P. volubilis leaves water extract was explored using in vitro and in silico approaches. High-Performance Liquid Chromatography coupled to Electrospray Ionisation and Quadrupole Time-of-Flight Mass Spectrometry (HPLC-ESI- QTOF-MS-MS) analysis of the water extract from the leaves was used to characterise the chemical composition of the plant and allowed identification of some flavonoids, such as astragalin, and some phenolic compounds. Then, high-speed counter current chromatography (HSCCC) was used to fractionate the ethyl acetate partition obtained from the water extract from the leaves.Results and DiscussionThe presence of flavonoids derived from kaempferol was confirmed and astragalin was isolated for the first time in P. volubilis. The P. volubilis water infusion, ethyl acetate extract and the isolated astragalin exhibited anti-bacterial activity against H. pylori J99 and two clinical isolates (e.g., minimum inhibitory concentrations of 0.53, 0.51 and 0.49 μg/mL, respectively, for clarithromycin-resistant clinical isolate SSR366). Then, using molecular docking for potential protein targets for H. pylori, it was verified that astragalin could interact with these proteins by in silico analysis.ConclusionThese findings highlight that P. volubilis and astragalin produce a bacteriostatic activity against H. pylori and may have potential to be used in treatment against H. pylori, after further research.

Abstract Climate change is a global concern, with far-reaching implications for biodiversity and ecosystems. Understanding impact on species distribution is crucial for effective conservation strategies. The aims of this study were to evaluate the projected effects of climate change on the potential distribution of Manihot species endemic to Northeast Brazil and estimate the presence of climate suitability within protected areas in the future. We used ecological niche models to assess the potential distribution of 11 endemic species, providing predictions of current and future scenarios using an optimistic and pessimistic climate change scenario. The results revealed that in the optimistic scenario, 45% of the species may experience a partial reduction in their potential distribution range by 2100, and this percentage increases to 54% in the pessimistic scenario. Other species, on the other hand, will increase their potential distribution. The climatically suitable area for most species will be inserted in some protected areas, but species with limited current distribution and decreasing potential range must be prioritized for conservation. This study provides valuable information about the future potential distribution of endemic species of Manihot.

O Brasil é um país com grande biodiversidade. Em sua flora, é encontrada a espécie Jatropha gossypiifolia L., conhecida popularmente como “pinhão-roxo”, “pião-roxo”, “pião-preto”, “jalapão”, “erva purgante” e “mamoninha”. Essa planta é muito utilizada na medicina popular do Brasil e de outros países por apresentar vários efeitos terapêuticos em decorrência da composição química da planta, onde é possível encontrar ácidos orgânicos, alcaloides, esteroides, flavonoides, taninos, fenóis, saponinas, entre outros constituintes. Este estudo teve como objetivo analisar os usos terapêuticos, a atividade farmacológica e a toxicologia da Jatropha gossypiifolia L. com base na literatura. A metodologia consistiu em selecionar artigos das bases de dados Pubmed, Google Scholar, Scielo e BVS e realizar uma análise através dos critérios de inclusão e exclusão. Através desse estudo, foi observado que o “pinhão-roxo” apresenta efeitos terapêuticos como: antimicrobiano, cicatrizante, diurético, analgésico, anti-hipertensivo, anti-inflamatório, entre outros citados no estudo, incluindo alguns contraditórios, como o antidiarreico e purgante. Apesar dos efeitos citados, a toxicologia da planta é um fator importante, destacando-se principalmente o látex presente no caule que apresenta atividade tóxica, além de alguns estudos indicarem também citotoxicidade e genotoxicidade. A J. gossypiifolia é uma planta que apresenta diversas propriedades farmacológicas que podem ser benéficas ou prejudiciais à saúde, sendo necessária uma análise mais aprofundada dos seus efeitos.

A new ketone, volubilisone A (1), along with seven known compounds including trans-methyl-p-coumarate (2), methyl-p-methoxybenzoate (3), coniferyl aldehyde (4), methyl ferulate (5), ferulic acid (6), p-coumaric acid (7), and protocatechuic acid (8), were isolated from the seed shells of Plukenetia volubilis L. (Euphorbiaceae). The structure of the new hexenone derivative was elucidated by chemical and physical evidence. [ABSTRACT FROM AUTHOR]

Acalypha australis L. 1753 is a potherb popular among Asian populations and is a traditional herbal medicine. In the current study, the overall genetic diversity of A. australis still needs to be better. Here, we assembled and characterized the complete plastome of A. australis. The plastome is 168,885 bp in length with a large single-copy (LSC) of 94,576 bp, a small single-copy (SSC) of 19,715 bp, and two copies of inverted repeat region (IRs) of 27,297 bp each. The overall GC content is 34.9%. The plastome contains 127 genes, including 83 protein-coding genes, 36 tRNA genes, and eight rRNA genes. Phylogenomic analysis of the representative species of Euphorbiaceae showed that A. australis and A. hispida formed a monophyletic sister clade. The results of this study will support further research on the evolution and conservation of the Euphorbiaceae species; they will benefit pharmaceutical applications and ornamentation of the medicinal plant A. australis.

Bioassay-guided fractionation led to the isolation of three new spirocyclic terpenoid compounds from Euphorbia amygdaloides L., named Zagrosin I–III. Their structures were identified by 1D and 2D NMR (1H NMR, 13C NMR, DEPT 135, HMBC, and HSQC-TOCSY) and LC-MS-MS spectrometry. The cytotoxicity of the isolated spirocyclic terpenoids (Zagrosin I-III) was assessed against human breast cancer (MCF-7), human fibrosarcoma (HT1080), and normal human foreskin fibroblast cells with MTT assays (24, 48, and 72 h treatments). The FITC-Annexin V apoptosis flow cytometry assays and cell cycle analysis were performed for Zagrosin I–III.These isolated compounds were identified as: (9)-8a-((benzoyloxy)methyl)-2-methoxy-4,9-dimethyltetrahydro-4H,5H-2,4a-methanobenzo[d] [1,3] dioxine-4-carboxylate (Zagrosin I), ((9)-4-hydroxy-2-methoxy-4,9-dimethyltetrahydro-4H,8aH-2,4a-methanobenzo[d] [1,3] dioxin-8a-yl) methyl benzoate (Zagrosin II), and (9)-2-methoxy-4,9-dimethyl-8a-(phenoxy methyl) tetrahydro-4H,5H-2,4a-methanobenzo[d][1,3]dioxin-4-yl 4-methylpentanoate (Zagrosin III).The IC50 of Zagrosin I on 48-h-treated MCF-7 was calculated as 1.5 μg/mL. Zagrosin II and III exhibited cytotoxicity on 48-h-treated MCF-7 with IC50s of 14.04 and 12.50 μg/mL, respectively. The IC50 of Zagrosin I on human fibrosarcoma (HT1080) was 115.5 μg/mL, Zagrosin III, 16.81 μg/mL (48 h treatment), and Zagrosin II, 142.7 μg/mL (72 h treatment). Zagrosin I-III exhibited significant cytotoxicity against the MCF-7 cell line and human fibrosarcoma (HT1080), with the mechanism of early and late apoptosis affecting cells mostly in G0/G1 fallowed by S and G2 phases. MCF-7 had a higher rate of phosphatidyl serine exposure on the cell membrane than two other studied cells. The cytotoxicity on normal human foreskin fibroblasts was low. Zagrosin I-III can be considered an effective chemical backbone for anticancer drug development.Abbreviations: 2D NMR: Two-Dimensional Nuclear Magnetic Resonance Spectroscopy; API: Atmospheric Pressure Ionization; DEPT: Distortionless Enhancement by Polarization Transfer; ELISA: Enzyme-Linked Immunosorbent assay; ERK: Extracellular signal-regulated kinase; ESI: Electrospray ionization; FBS: Fetal Bovine Serum; FITC: Fluorescein isothiocyante; fr: fraction; HMBC: Heteronuclear Multiple Bond Correlation; HPLC: High-Performance Liquid Chromatography; HSQC: Heteronuclear Single Quantum Coherence; HT1080: Human fibrosarcoma cell line; IC50: Half-maximal inhibitory concentration; MCF-7: Human breast cancer cell line; MHz: Megahertz; MTT: 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; NMR: Nuclear Magnetic Resonance; PBS: Phosphate Buffered Saline; PI: Propidium Iodide; ppm: Part Per Million; Rf: Retention Factor; TLC: Thin-layer chromatography; TOCSY: Total Correlation Spectroscopy; VLC: Vacuum Liquid Chromatography.

Despite efforts, breast cancer remains associated with a high incidence and mortality rate. Ricinodendron heudelotii also known as "Njansang," is a plant used for cancer treatment. While several reports on the anticancer potential of its leaves exist, little is known about its seed oil. This study aimed to evaluate the in vitro and in vivo anti-breast cancer activity of "Njansang" seed oil. The inhibitory effect of "Njansang" seed oil was determined using MTT and CCK-8 dye reduction assays. Breast cancerwas inducedwithDMBA and promoted with E2V (1mg/kg) for 4 weeks in ovariectomized rats (menopausal condition). Evaluated parameters included tumor incidence, tumor mass and volume, histopathology, breast cancer biomarker CA 15-3, antioxidant status (CAT, GSH, MDA, NO, SOD), TNF-α and INFγ levels, lipid profile (total cholesterol, LDL-cholesterol, triglycerides and HDL-cholesterol), as well as toxicity parameters (ALT, AST, creatinine). "Njansang" oil significantly reduced the growth of ER+ (MCF-7) and triple negative (MDA-MB 231) adenocarcinoma cells in vitro as well as tumor incidence, tumor mass and CA 15-3 levels in vivo. It exhibited antioxidant activity, characterized by an increase in SOD and catalase activities, GSH levels and decreased MDA levels compared to the DMBA group. TNF-α and INF-γ levels were reduced following oil treatment, while total cholesterol, LDL-cholesterol and triglyceride levelswere reduced. The aforementioned findings confirm the protective effects of "Njansang" oil on induced breast cancer in ovariectomized rats. [ABSTRACT FROM AUTHOR]

The Mediterranean Basin is a hotspot of animal and plant diversity. Contrary to the Balkan and Iberian Peninsulas that were subject of many phylogeographic studies in past decades, the Apennine Peninsula and, in particular, the diversification of its flora has been neglected in contemporary phylogenetic studies. The few available studies showed a complex pattern of north–south differentiation of genetic diversity in Italy, both among the Alps, the Apuan Alps, and the Apennines, as well as within the Apennines. Here, we explore phylogeographic patterns within recently described Euphorbia adriatica , distributed in the central and northern Apennine Peninsula, the southern margin of the Eastern Alps and the north-western Balkan Peninsula, and its relationship to the southern Italian E. japygica. Our integrative approach using nuclear ITS sequences, amplified fragment length polymorphisms, relative genome size estimation combined with chromosome counting, as well as multivariate morphometrics inferred a weak genetic differentiation that only partly corresponds to the morphological differentiation. Whereas all southern populations have hairy capsules characteristic for E. japygica , only those in south-eastern Italy (Puglia and Basilicata) are genetically divergent. There are, however, additional morphological characters that differentiate them from other populations. Our data are thus in favour of recognizing a single species, E. japygica , which includes E. adriatica that should be treated as a subspecies, named E. japygica subsp. prostrata. We provide a revised taxonomic treatment for E. japygica , including the typification. Our study shows the necessity of further in-depth investigations of diversification of Italian biota. [ABSTRACT FROM AUTHOR]

A new 1,6-seco-norisoprenoid, named croyanhuin G (1), together with ten known norisoprenoids (2–11), was isolated from the leaves and twigs of Croton yanhuii Y. T. Chang (Euphorbiaceae). The structure of this new compound was characterized and identified by HR-ESI-MS, IR, and 1D and 2D NMR spectroscopic techniques. Compound 1 was evaluated for its cytotoxic activity against HeLa cell line. [ABSTRACT FROM AUTHOR]

The galls can offer shelter, protection, and an adequate diet for the gall-inducing organisms. Herein, we evaluated the structure of Manihot esculenta leaves and galls induced by Iatrophobia brasiliensis in order to identify metabolic and cell wall composition changes. We expected to find a complex gall with high primary metabolism in a typical nutritive tissue. Non-galled leaves and galls were subjected to anatomical, histochemical, and immunocytochemical analyses to evaluate the structural features, primary and secondary metabolites, and glycoproteins, pectins, and hemicelluloses in the cell wall. The gall is cylindric, with a uniseriate epidermis, a larval chamber, and a parenchymatic cortex divided into outer and inner compartments. The outer compartment has large cells with intercellular spaces and stocks starch and is designated as storage tissue. Reducing sugars, proteins, phenolic compounds, and alkaloids were detected in the protoplast of inner tissue cells of galls, named nutritive tissue, which presents five layers of compact small cells. Cell walls with esterified homogalacturonans (HGs) occurred in some cells of the galls indicating the continuous biosynthesis of HGs. For both non-galled leaves and galls, galactans and xyloglucans were broadly labeled on the cell walls, indicating a cell growth capacity and cell wall stiffness, respectively. The cell wall of the nutritive tissue had wide labeling for glycoproteins, HGs, heteroxylans, and xyloglucans, which can be used as source for the diet of the galling insect. Manihot esculenta galls have compartments specialized in the protection and feeding of the galling insect, structured by nutritive tissue rich in resource compounds, in the cell walls and protoplast. [ABSTRACT FROM AUTHOR]

Copyright of Environmental & Social Management Journal / Revista de Gestão Social e Ambiental is the property of Environmental & Social Management Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Spodoptera frugiperda is the main pest of maize. One of the alternatives proposed for its control is the implementation of products of botanical origin, such as those derived from Ricinus communis. In this work, the insecticidal and insectistatic activities of methanolic and hydrolyzed methanolic extracts of the aerial parts of R. communis and kaempferol against S. frugiperda are evaluated. The methanolic extract presented a larval mortality rate of 55% and an accumulated mortality rate of 65% starting at 4000 ppm, with LC50 values of 3503 (larvae) and 2851 (accumulated); meanwhile, from a concentration of 1000 ppm, a decrease in pupa weight at 24 h of 20.5 mg was observed when compared to the control. The hydrolyzed methanolic extract presented a larval mortality and accumulated mortality rate of 60% from a concentration of 1000 ppm, and a decrease in pupa weight at 24 h of 35.31 mg was observed, when compared to the control. For the compound kaempferol 3-β-D-glucopyranoside, a larval mortality rate of 65% and an accumulated mortality rate of 80% were observed from 800 ppm, with LC50 values of 525.2 (larvae) and 335.6 ppm (accumulated); meanwhile, at 300 ppm, a decrease in pupa weight of 25.59 mg after 24 h was observed when compared to the control. [ABSTRACT FROM AUTHOR]

A new clerodane diterpenoid, crotolanin A (1), along with three known clerodane diterpenoids, crotoeurin B (2), teucvidin (3) and teucvin (4), was isolated from the ethanol extract of the leaves and twigs of Croton lachnocarpus Benth. Their structures were identified by extensive NMR spectroscopic and HRESIMS analyses. The dopaminergic neuroprotective activity of compounds 1-4 was tested by using transgenic Caenorhabditis elegans pathological model. Compound 2 alleviated dopaminergic neuron degeneration of worms induced by 6-hydroxydopamine (6-OHDA) that represented a potential therapy for Parkinson's disease (PD). [ABSTRACT FROM AUTHOR]

Helicases, motor proteins present in both prokaryotes and eukaryotes, play a direct role in various steps of RNA metabolism. Specifically, SF2 RNA helicases, a subset of the DEAD-box family, are essential players in plant developmental processes and responses to biotic and abiotic stresses. Despite this, information on this family in the physic nut (Jatropha curcas L.) remains limited, spanning from structural patterns to stress responses. We identified 79 genes encoding DEAD-box RNA helicases (JcDHX) in the J. curcas genome. These genes were further categorized into three subfamilies: DEAD (42 genes), DEAH (30 genes), and DExH/D (seven genes). Characterization of the encoded proteins revealed a remarkable diversity, with observed patterns in domains, motifs, and exon–intron structures suggesting that the DEAH and DExH/D subfamilies in J. curcas likely contribute to the overall versatility of the family. Three-dimensional modeling of the candidates showed characteristic hallmarks, highlighting the expected functional performance of these enzymes. The promoter regions of the JcDHX genes revealed potential cis-elements such as Dof-type, BBR-BPC, and AP2-ERF, indicating their potential involvement in the response to abiotic stresses. Analysis of RNA-Seq data from the roots of physic nut accessions exposed to 150 mM of NaCl for 3 h showed most of the JcDHX candidates repressed. The protein–protein interaction network indicated that JcDHX proteins occupy central positions, connecting events associated with RNA metabolism. Quantitative PCR analysis validated the expression of nine DEAD-box RNA helicase transcripts, showing significant associations with key components of the stress response, including RNA turnover, ribosome biogenesis, DNA repair, clathrin-mediated vesicular transport, phosphatidyl 3,5-inositol synthesis, and mitochondrial translation. Furthermore, the induced expression of one transcript (JcDHX44) was confirmed, suggesting that it is a potential candidate for future functional analyses to better understand its role in salinity stress tolerance. This study represents the first global report on the DEAD-box family of RNA helicases in physic nuts and displays structural characteristics compatible with their functions, likely serving as a critical component of the plant's response pathways. [ABSTRACT FROM AUTHOR]

Plant extracts are actively being used worldwide due to the presence of biologically active constituents helping in the preservation of food, and to aid against various diseases owing to their antimicrobial and antioxidant potential. The present research work was carried out to investigate the phytochemical constituents, antimicrobial activity, and antioxidant activity of different extracted samples of Euphorbia parviflora. Anti-microbial studies were carried out by Agar well diffusion while the DPPH method was employed for investigating anti-oxidant activity. Three samples from methanol, chloroform, and ethyl acetate extract were tested against five different bacterial strains comprising two species from Gram-negative bacteria i.e., Staphylococcus aureus and Bacillus subtilis and three species from Gram-positive bacteria i.e. Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia along two fungal strains i.e. Candida albicans and Aspergillus niger. The results of the qualitative phytochemical analysis showed that methanolic, chloroformic, and ethylacetate extract of Euphorbia parviflora consist of alkaloids, reducing sugars, flavonoids, terpenoids, tannins, and saponins. The total phenol and flavonoid content of E. parviflora showed that the methanolic extract of E. parviflora had a significantly higher total phenolic content (53.73 ± 0.30 mg of GAE/g) and flavonoid content (44.62 ± 0.38 mg of than other extracts. The content of total phenolic and flavonoids was more in methanolic extract as compared to other extracts of E. prolifera. The HPLC analysis showed that in the chloroform extract of E. parviflora Cinnamic acid (4.32 ± 2.89 mg/g) was dominant, in methanol extract quercetin (3.42 ± 2.89 mg/g) was dominant and in ethyl acetate extract of E. parviflora catechin (4.44 ± 2.89 mg/g) was found dominant. The antimicrobial activity revealed that amongst all the extracts the highest antibacterial activity was shown by methanolic extract against B. subtilis and Staphylococcus aureus as compared to the other extracts. The antioxidant activity revealed that methanolic extract of E. parviflora demonstrated higher antioxidant activity (82.42 ± 0.02) followed by chloroform extract (76.48 ± 0.08) at 150 µg/mL. The aim of this study was primarily to evaluate the potential of this plant as a reliable source of antimicrobials and antioxidants that may be used for the treatment of various infectious diseases in the future. The study provides evidence that this plant can act as a reliable source of antimicrobial and antioxidant agents and might be used against several infectious diseases. [ABSTRACT FROM AUTHOR]

Plant mediated green synthetic route for silver and gold nanoparticles (AgNPs & AuNPs) from four plant families: Phyllanthaceae, Lamiaceae, Rutaceae and Euphorbiaceae and their therapeutic application of are summarized in the present review article. Silver and gold nanoparticles prepared from the mentioned plant families were visualized with various therapeutic applications viz., anti-microbial, ant-bacterial, anti-cancer and anti-oxidant activities etc. Owing to size and shapes, NPs green synthesized from various plants like Ricinus communis (Ag), Tectona grandis (Ag), Plectranthus amboinicus (Ag, Au), Phyllanthus emblica (Ag, Au), Ricinu communis (Ag), Leucas aspera (Ag, Au), Acalypha indica Linn. (Ag, Au) were envisioned with innumerable curative applications such as antimicrobial, anticancer, antibacterial, anti-oxidant, cytotoxicity, haemolytic and stimulating effect etc. In the current review, eco-friendly green synthetic itineraries for synthesis of AgNPs and AuNPs are mainly focused. Phyto-components present in plants such as anti-oxidant molecules, terpenes, alkaloids, coumarins like quercetin, d -allose, 1-valine, ocosenamide molecules etc. are the responsible reducing agents for the capping reaction (Ag+ to Ag0 and Au3+ to Au0) of nanoparticle from metal precursors like AgNO 3 and HAuCl 4. [Display omitted] • Bio-synthesis of AgNPs and AuNPs from four plant families: Phyllanthaceae, Lamiaceae, Rutaceae and Euphorbiaceae are summarized. • AgNPs and AuNPs green synthesized from plants belonging to Phyllanthaceae, Lamiaceae, Rutaceae and Euphorbiaceae families contain various medicinal applications like antimicrobial, anticancer, antibacterial, anti-oxidant, cytotoxicity etc. • Phyto-compounds present in plants are responsible for capping reaction of nano-particles. [ABSTRACT FROM AUTHOR]

Cassava Lace Bugs (CLB) are native pest of cassava (Manihot esculenta Crantz, Euphorbiaceae) to the Neotropical Region, mainly in Brazil. On the other hand, East Java was the first region in Indonesia to record the presence of CLB in 2021, however, it has not been reported in other regions in Indonesia. Therefore, the very importance to recognise the occurrence of CLB in other regions in Indonesia. Based on this, the research has been carried out starting with a field survey, observing behaviour of insect in the field and identify morphologically in the laboratory. The survey results show that the infestation of CLB has been found in lowland, medium, and highland areas in Bali. Symptoms of infestation on the upper leaf surface are small yellow spots with brownish variations. Based on the identification key, CLB from Bali Indonesia, shows a characteristic of a head with a pair of frontal spines. Based on this evidence, the CLB insect can be identified as Vatiga illudens (Hemiptera: Heteroptera: Tingidae). It is the first report of novel distribution areas for V. illudens in Bali, Indonesia. The results of this research are important because V. illudens is one of the main pests of cassava. [ABSTRACT FROM AUTHOR]

Plukenetia volubilis L. has been documented as a new record for the first time in Java, Indonesia. The species is easily distinguished from the native species, P. corniculata Sm., by its exstipellate basilaminar-glands, long cylindrical column, and wingless fruit-lobes. Plukenetia volubilis is cultivated mainly in South America for its beneficial values as food and medicine and was recently introduced to Asia. However, its occurrence in Java has not been reported. We collected specimens from West Java (Depok City, Bandung Barat and Sumedang Regency) and East Java (Malang Regency). Morphological description, identification key, and photographs of the species are provided. [ABSTRACT FROM AUTHOR]

Two new rare trachylobane euphoratones A-B (1-2), together with five known diterpenoids (compounds 3-7), were isolated from the aerial parts of Euphorbia atoto. Their structures were unambiguously elucidated through HRESIMS, 1D and 2D NMR spectral analysis. Compounds 1, 3, 4 and 7 showed weak anti-inflammatory activities (IC50 77.49 ± 6.34, 41.61 ± 14.49, 16.00 ± 1.71 and 33.41 ± 4.52 μM, respectively), compared to the positive control quercetin (IC50 15.23 ± 0.65 μM). [ABSTRACT FROM AUTHOR]

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

ABSTRACT Manihot includes about 150 species with a Neotropical distribution. Wild species of Manihot constitute a plant genetic heritage that can be used in breeding programs for the cultivable species Manihot esculenta Crantz (cassava). However, wild populations, especially endemic ones, are under constant threat due to habitat destruction and climate change. The objective of this manuscript was to elaborate a taxonomic treatment of the endemic species of Manihot recorded in Northeast Brazil; determine the geographical distribution of species, richness, their conservation status, and solve typification problems. The study was based on the analysis of specimens deposited in national and international herbaria, as well as on field observations. Fifteen endemic species of Manihot were found, we propose lectotypes for five of them and one neotype. Most species are distributed in the state of Bahia, growing in Caatinga, Cerrado, and Atlantic Forest environments. Only M. breviloba was found in the states of Sergipe and Alagoas. The richest area was found in the Chapada Diamantina of the Espinhaço Range. In terms of conservation status, 80% of endemic species are in some threatened category, 13% are in the near threatened category and only 7% are in the least concern category.

Acalypha wilkesiana, commonly known as “Copper leaf” and belonging to the Euphorbiaceae family, is a tropical shrub renowned for its ornamental beauty and diverse cultural uses. Its leaves are frequently utilized by locals for various medicinal purposes. This study evaluated the proximate, mineral and vitamin compositions of Acalypha wilkesiana leaf. Proximate analysis was conducted using standard methods, while the mineral concentrations were determined by means of atomic absorption spectrophotometer, and vitamins were quantified using appropriate colorimetric and titration methods. The percentage proximate composition for moisture, ash, fiber, protein, fat, and carbohydrates were 13.75±1.75, 12.75±0.75, 8.00±0.60, 3.65±0.05, 12.90±0.10, and 48.95±0.45, respectively, while the energy value was 326±1.10 kcal/g. The mineral analysis revealed that the leaf is rich in sodium, potassium, phosphorus, magnesium, manganese, calcium and zinc, with concentrations (mg/g) of 3.96±0.05, 2.89±1.79, 2.73±0.25, 1.94±0.08, 1.40±1.01, 1.31±0.42 and 0.30±0.14, respectively. The fat soluble vitamins ranged from 4.12±0.26 (for vitamin D) ¬¬to 17.28±1.00 mg/kg (for vitamin E), while the water soluble vitamins ranged from 0.05±0.02 mg/100g (for vitamin B2) to 69.61±1.77 mg/kg (for vitamin C). The results show that Acalypha wilkesiana leaf is a good source of nutrients, minerals and vitamins, and could serve as a dietary supplement for addressing nutritional deficiencies.

Abstract Background Different herbal phytochemicals have potential in cancer treatment, Euphorbia genus has valuable secondary metabolites and is used in traditional medicine to treat various ailments. However, the specific constituents and biological activity of Euphorbia greenwayi remain largely unexplored. Results Euphorbia greenwayi aerial parts were extracted using methanol. Consequently, the methanol extract was then fractionated with solvents of different polarities viz., n-hexane, chloroform, and ethyl acetate. All were screened for their cytotoxic activity against different cell lines; MCF-7, HepG-2, and SW620. The n-hexane (HF) and chloroform (CF) fractions showed considerable activity against all tested cell lines especially MCF-7 with IC50 values at 18.6 ± 0.2 and 17.5 ± 0.6 μg/ml respectively. Therefore, a cell migration assay on the MCF-7 cell line was applied to both fractions as well as investigation and isolation of the main active constituents. Lupeol, β-sitosterol, and cycloartenol were isolated from the nonpolar fractions of E. greenwayi for the first time. Conclusions Euphorbia greenwayi aerial parts exhibit considerable anti-cancer effects via cytotoxicity. Three chemical constituents with promising cytotoxic activity are identified.

AbstractEuphorbia prostrata Aiton 1789, an annual herb, is native to tropical and subtropical Americas and was introduced into many parts of the Old-World including Korea. We determined the genomic characteristics of the complete chloroplast genome of E. prostrata with a de novo assembly strategy. The complete chloroplast genome was 162,858 bp long, and harbored 86 protein coding, 37 tRNA and 8 rRNA. The genome showed the typical quadripartite structure consisting of a large single copy (LSC) (90,580 bp), a small single copy (SSC) (18,570 bp) and a pair of inverted repeats (IRs) (26,854 bp). The phylogenetic tree inferred from chloroplast genomes of 25 taxa, which belong to Euphorbiaceae indicated that the Euphorbia is a monophyletic group and E. prostrata is closely related with E. humifusa, E. thymifolia and E. hirta, forming a well-supported clade. Our findings can be valuable for understanding the phylogenetic relationships and the evolution in Euphorbiaceae and will provide basic information for future studies on E. prostrata from genomic perspective.

Abstract Background In cellular organisms, inosine triphosphate pyrophosphatases (ITPases) prevent the incorporation of mutagenic deaminated purines into nucleic acids. These enzymes have also been detected in the genomes of several plant RNA viruses infecting two euphorbia species. In particular, two ipomoviruses produce replicase-associated ITPases to cope with high concentration of non-canonical nucleotides found in cassava tissues. Method Using high-throughput RNA sequencing on the wild euphorbia species Mercurialis perennis, two new members of the families Potyviridae and Secoviridae were identified. Both viruses encode for a putative ITPase, and were found in mixed infection with a new partitivirid. Following biological and genomic characterization of these viruses, the origin and function of the phytoviral ITPases were investigated. Results While the potyvirid was shown to be pathogenic, the secovirid and partitivirid could not be transmitted. The secovirid was found belonging to a proposed new Comovirinae genus tentatively named "Mercomovirus", which also accommodates other viruses identified through transcriptome mining, and for which an asymptomatic pollen-associated lifestyle is suspected. Homology and phylogenetic analyses inferred that the ITPases encoded by the potyvirid and secovirid were likely acquired through independent horizontal gene transfer events, forming lineages distinct from the enzymes found in cassava ipomoviruses. Possible origins from cellular organisms are discussed for these proteins. In parallel, the endogenous ITPase of M. perennis was predicted to encode for a C-terminal nuclear localization signal, which appears to be conserved among the ITPases of euphorbias but absent in other plant families. This subcellular localization is in line with the idea that nucleic acids remain protected in the nucleus, while deaminated nucleotides accumulate in the cytoplasm where they act as antiviral molecules. Conclusion Three new RNA viruses infecting M. perennis are described, two of which encoding for ITPases. These enzymes have distinct origins, and are likely required by viruses to circumvent high level of cytoplasmic non-canonical nucleotides. This putative plant defense mechanism has emerged early in the evolution of euphorbias, and seems to specifically target certain groups of RNA viruses infecting perennial hosts. Graphical Abstract

Cây Dầu lai có củ (Jatropha podagrica Hook. – Euphorbiaceae) được dùng trong y học cổ truyền để chữa bệnh táo bón, nhiễm trùng da, vàng da, và sốt. Thành phần hóa học chủ yếu là các hợp chất nhóm flavonoid, coumarin, acid phenol, diterpenoid, peptide, và steroid. Một số nghiên cứu đã cho biết các tác dụng dược lý tiềm năng của cây Dầu lai có củ như hoạt tính kháng khuẩn, kháng ung thư, chống oxy hóa, ức chế sự phát triển của côn trùng, ức chế thần kinh cơ và hạ huyết áp. Cho đến nay, Dược điển Việt Nam V vẫn chưa có chuyên luận về dược liệu Dầu lai có củ. Do đó, mục tiêu của nghiên cứu này là xác định các đặc điểm thực vật học của cây Dầu lai có củ. Đặc điểm hình thái, vi phẫu, bột dược liệu của lá, thân và rễ được mô tả chi tiết. Kết quả của nghiên cứu này hữu ích cho việc xác định và kiểm nghiệm dược liệu cũng như xây dựng chuyên luận Dầu lai có củ trong Dược điển Việt Nam.