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2 results on '"Eva-Maria C, Moench"'

1. Author Correction: LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding

Author

Kerstin U. Ludwig, Ricarda M. Schmithausen, David Li, Max L. Jacobs, Ronja Hollstein, Katja Blumenstock, Jana Liebing, Mikołaj Słabicki, Amir Ben-Shmuel, Ofir Israeli, Shay Weiss, Thomas S. Ebert, Nir Paran, Wibke Rüdiger, Gero Wilbring, David Feldman, Bärbel Lippke, Nina Ishorst, Lara M. Hochfeld, Eva C. Beins, Ines H. Kaltheuner, Maximilian Schmitz, Aliona Wöhler, Manuel Döhla, Esther Sib, Marius Jentzsch, Eva-Maria C. Moench, Jacob D. Borrajo, Jonathan Strecker, Julia Reinhardt, Brian Cleary, Matthias Geyer, Michael Hölzel, Rhiannon Macrae, Markus M. Nöthen, Per Hoffmann, Martin Exner, Aviv Regev, Feng Zhang, and Jonathan L. Schmid-Burgk

Subjects
Biomedical Engineering, Molecular Medicine, Bioengineering, Applied Microbiology and Biotechnology, Biotechnology
Published
2022
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2. LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding

Author

Kerstin U, Ludwig, Ricarda M, Schmithausen, David, Li, Max L, Jacobs, Ronja, Hollstein, Katja, Blumenstock, Jana, Liebing, Mikołaj, Słabicki, Amir, Ben-Shmuel, Ofir, Israeli, Shay, Weiss, Thomas S, Ebert, Nir, Paran, Wibke, Rüdiger, Gero, Wilbring, David, Feldman, Bärbel, Lippke, Nina, Ishorst, Lara M, Hochfeld, Eva C, Beins, Ines H, Kaltheuner, Maximilian, Schmitz, Aliona, Wöhler, Manuel, Döhla, Esther, Sib, Marius, Jentzsch, Eva-Maria C, Moench, Jacob D, Borrajo, Jonathan, Strecker, Julia, Reinhardt, Brian, Cleary, Matthias, Geyer, Michael, Hölzel, Rhiannon, Macrae, Markus M, Nöthen, Per, Hoffmann, Martin, Exner, Aviv, Regev, Feng, Zhang, and Jonathan L, Schmid-Burgk

Subjects
COVID-19 Testing, Molecular Diagnostic Techniques, COVID-19, Humans, Nucleic Acid Amplification Techniques
Abstract

Frequent testing of large population groups combined with contact tracing and isolation measures will be crucial for containing Coronavirus Disease 2019 outbreaks. Here we present LAMP-Seq, a modified, highly scalable reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Unpurified biosamples are barcoded and amplified in a single heat step, and pooled products are analyzed en masse by sequencing. Using commercial reagents, LAMP-Seq has a limit of detection of ~2.2 molecules per µl at 95% confidence and near-perfect specificity for severe acute respiratory syndrome coronavirus 2 given its sequence readout. Clinical validation of an open-source protocol with 676 swab samples, 98 of which were deemed positive by standard RT-qPCR, demonstrated 100% sensitivity in individuals with cycle threshold values of up to 33 and a specificity of 99.7%, at a very low material cost. With a time-to-result of fewer than 24 h, low cost and little new infrastructure requirement, LAMP-Seq can be readily deployed for frequent testing as part of an integrated public health surveillance program.

Published
2020

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