Search

Your search keyword '"Evandro Ricardo da Costa Colares"' showing total 2 results
Start Over Author "Evandro Ricardo da Costa Colares"
2 results on '"Evandro Ricardo da Costa Colares"'

1. Wastewater-based epidemiological investigation of SARS-CoV-2 in Porto Alegre, Southern Brazil

Author

Bruno Aschidamini Prandi, Arthur Tonietto Mangini, Waldemir Santiago Neto, André Jarenkow, Lina Violet-Lozano, Aline Alves Scarpellini Campos, Evandro Ricardo da Costa Colares, Paula Regina de Oliveira Buzzetto, Camila Bernardes Azambuja, Lisiane Correa de Barros Trombin, Margot de Souza Raugust, Rafaela Lorenzini, Alberto da Silva Larre, Caroline Rigotto, Fabrício Souza Campos, and Ana Cláudia Franco

Subjects
SARS-CoV-2, Wastewater based epidemiology, Social restrictions, RT-qPCR, Ultracentrifugation, Veterinary medicine, SF600-1100, Medicine
Abstract

Wastewater-based epidemiology (WBE) may be successfully used to comprehensively monitor and determine the scale and dynamics of some infections in the community. We monitored severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in raw wastewater samples from Porto Alegre, Southern Brazil. The samples were collected and analyzed every week between May 2020 to May 2021. Meanwhile, different social restrictions were applied according to the number of hospitalized patients in the region. Weekly samples were obtained from two wastewater treatment plants (WWTP), named Navegantes and Serraria. To determine the SARS-CoV-2 RNA titers in wastewater, we performed RT-qPCR analysis targeting the N gene (N1). The highest titer of SARS-CoV-2 RNA was observed between epidemiological weeks (EWs) 33–37 (August), 42–43 (October), 45–46 (November), 49–51 (December) in 2020, and 1–3 (January), 7–13 (February to March) in 2021, with viral loads ranging from 1 × 106–3 × 106 genomic copies/Liter. An increase in positive confirmed cases followed such high viral loads. Depending on the sampling method used, positive cases increased in 6–7 days and 15 days after the rise of viral RNA titers in wastewater, with composite sampling methods showing a lower time lag and a higher resolution on the analyses. The results showed a direct relation between strict social restrictions and the loads of detected RNA reduction in wastewater, corroborating the number of confirmed cases. Differences in viral loads between different sampling points and methods were observed, as composite samples showed more stable results during the analyzed period. Besides, viral loads obtained from samples collected at Serraria WWTP were consistently higher than the ones obtained at Navegantes WWTP, indicating differences in local dynamics of SARS-CoV-2 spread in different regions of Porto Alegre. In conclusion, wastewater sampling to monitor SARS-CoV-2 is a robust tool to evaluate the viral loads contributing to hospitalized patients’ data and confirmed cases. In addition, SARS-CoV-2 detection in sewage may inform and alert the government when there are asymptomatic or non-tested patients.

Published
2022
Full Text
View/download PDF

2. Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants

Author

Allan Guedes Pozzebon, Viviane Girardi, Kristina D. Mena, Ana Paula Campos Westphalen, Fernando Rosado Spilki, Rafael Newton Zaneti, Ramiro Gonçalves Etchepare, and Evandro Ricardo da Costa Colares

Subjects
Environmental Engineering, 010504 meteorology & atmospheric sciences, Sanitation, Pneumonia, Viral, Sewage, Wastewater, 010501 environmental sciences, medicine.disease_cause, Risk Assessment, 01 natural sciences, Article, Betacoronavirus, Occupational Exposure, Environmental health, Wastewater treatment plants, Pandemic, medicine, Humans, Environmental Chemistry, Pandemics, Waste Management and Disposal, Risk management, 0105 earth and related environmental sciences, Coronavirus, Novel coronavirus, SARS-CoV-2, business.industry, Transmission (medicine), Emergency response plans, COVID-19, Pollution, Quantitative microbial risk assessment, Sewage treatment, Coronavirus Infections, business, Viral load
Abstract

Faecal-oral transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is yet to be validated, but it is a critical issue and additional research is needed to elucidate the risks of the novel coronavirus in sanitation systems. This is the first study that investigates the potential health risks of SARS-CoV-2 in sewage to wastewater treatment plant (WWTP) workers. A quantitative microbial risk assessment (QMRA) is applied for three COVID-19 scenarios (moderate, aggressive and extreme) to study the effects of different stages of the pandemic in terms of percentage of infected population on the probability of infection to WWTP workers. A dose-response model for SARS-CoV-1 (as a surrogate pathogen) is assumed in the QMRA for SARS-CoV-2 using an exponential model with k = 4.1 × 102. Literature data are incorporated to inform assumptions for calculating the viral load, develop the model, and derive a tolerable infection risk. Results reveal that estimates of viral RNA in sewage at the entrance of WWTPs ranged from 4.14 × 101 to 5.23 × 103 GC·mL−1 (viable virus concentration from 0.04 to 5.23 PFU·mL−1, respectively). In addition, estimated risks for the aggressive and extreme scenarios (2.6 × 10−3 and 1.3 × 10−2, respectively) were likely to be above the derived tolerable infection risk for SARS-CoV-2 of 5.5 × 10−4 pppy, thus reinforcing the concern of sewage systems as a possible transmission pathway of SARS-CoV-2. These findings are helpful as an early health warning tool and in prioritizing upcoming risk management strategies, such as Emergency Response Plans (ERPs) for water and sanitation operators during the COVID-19 and future pandemics., Graphical abstract Unlabelled Image, Highlights • Viral loads and infectivity of SARS-CoV-2 in sewage affluent to WWTPs were estimated. • QMRA estimated the risk of infection for WWTP workers in three COVID-19 scenarios. • QMRA risk estimates will provide critical input for risk management decisions. • Wastewater operators can benefit from QMRA-informed Emergency Response Plans.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results