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Advances in cryobiology techniques commonly target either the cooling or the warming cycle, while little thought has been given to ≪repair≫ protocols applicable during cold storage. In particular, crystallization is the dominant threat to cryopreserved samples but proceeds from small nuclei that are innocuous if further growth is forestalled. To this end, we propose a laser editing technique that locally heats individual crystals above their melting point by a focused nanosecond pulse, followed by amorphization during rapid resolidification. As a reference, we first apply the approach to ice crystals in cryoprotected solution and use Raman confocal mapping to study the deactivation of crystalline order. Then, we examine dimethyl sulfoxide trihydrate crystals that can germinate at low temperatures in maximally freeze concentrated regions, as commonly produced by equilibrium cooling protocols. We show how to uniquely identify this phase from Raman spectra and evidence retarded growth of laser-edited crystals during warming. [ABSTRACT FROM AUTHOR]

Frequency doubling of multimode diode-pumped GRIN-fiber Raman laser with improved beam quality (M 2 =1.9-2.6 depending on configuration) in a simple single-pass scheme with 5-mm PPLN crystal is studied. After scheme optimization and elimination of back reflection and crystal heating effects, an efficient conversion into blue spectral range with output power of about 0.4 W@488 nm and 0.64 W@477 nm has been demonstrated.