1. Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie
- Author
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A. Lai, M. G. Cancedda, Ciriaco Ligios, Giuseppe Marruchella, Caterina Sorteni, Caterina Maestrale, Di Guardo G, Roberto Chiocchetti, F. Demontis, Cancedda MG, Di Guardo G, Chiocchetti R, Demontis F, Marruchella G, Sorteni C, Maestrale C, Lai A, and Ligios C.
- Subjects
Palatine tonsil ,PrPSc Proteins ,Prions ,Lymphoid Tissue ,animal diseases ,Immunology ,Scrapie ,Pathogenesis ,Biology ,Microbiology ,prion ,Peyer's Patches ,Virology ,medicine ,Animals ,Sheep ,Experimental sheep scrapie ,Spinal cord ,nervous system diseases ,medicine.anatomical_structure ,Lymphatic system ,nervous system ,SARDA SHEEP ,Insect Science ,Tonsil ,Medulla oblongata - Abstract
Atypical and classical scrapie-infected sheep brain tissue was monolaterally injected into the tonsils of lambs to investigate their role as a prion entry point. We first detected classical PrP Sc within the inoculated tonsil and in the ipsilateral retropharyngeal lymph node at 3 months postinoculation (p.i.). At 7 months p.i., PrP Sc colonized other lymphoid tissues bilaterally, including ileal Peyer's patches. The earliest PrP Sc deposition within the brain was ipsilaterally observed at 9 months p.i. in the substantia reticularis of the medulla oblongata. At 12 months p.i., PrP Sc deposition was present bilaterally in the nucleus parasympathicus nervi vagi, as well as in the intermediolateral cell column of the thoracolumbar spinal cord. No PrP Sc was detected in the lambs inoculated with atypical scrapie. These findings suggest that neuroinvasion may naturally occur from the tonsil after a widespread prion replication within the lymphoid tissues during classical scrapie only, thus mimicking the pathogenesis after oral ingestion.
- Published
- 2014
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