Search

Your search keyword '"Füzik, Tibor"' showing total 40 results
Start Over Author "Füzik, Tibor"
40 results on '"Füzik, Tibor"'

3. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi

Author

Homola, Miroslav, primary, Büttner, Carina R., additional, Füzik, Tibor, additional, Křepelka, Pavel, additional, Holbová, Radka, additional, Nováček, Jiří, additional, Chaillet, Marten L., additional, Žák, Jakub, additional, Grybchuk, Danyil, additional, Förster, Friedrich, additional, Wilson, William H., additional, Schroeder, Declan C., additional, and Plevka, Pavel, additional

Published
2024
Full Text
View/download PDF

9. Structure and replication cycle of a virus infecting climate-modulating alga Emiliania huxleyi

Author

Structural Biochemistry, Sub Structural Biochemistry, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten L., Žák, Jakub, Grybchuk, Danyil, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., Plevka, Pavel, Structural Biochemistry, Sub Structural Biochemistry, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten L., Žák, Jakub, Grybchuk, Danyil, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., and Plevka, Pavel

Published
2024

10. The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation.

Author

Anastasina, Maria, Füzik, Tibor, Domanska, Aušra, Pulkkinen, Lauri Ilmari Aurelius, Šmerdová, Lenka, Formanová, Petra Pokorná, Straková, Petra, Nováček, Jiří, Růžek, Daniel, Plevka, Pavel, and Butcher, Sarah Jane

Subjects
*TICK-borne encephalitis viruses, *FLAVIVIRUSES, *ATOMIC models
Abstract

We present structures of three immature tick-borne encephalitis virus (TBEV) isolates. Our atomic models of the major viral components, the E and prM proteins, indicate that the pr domains of prM have a critical role in holding the heterohexameric prM3E3 spikes in a metastable conformation. Destabilization of the prM furin-sensitive loop at acidic pH facilitates its processing. The prM topology and domain assignment in TBEV is similar to the mosquito-borne Binjari virus, but is in contrast to other immature flavivirus models. These results support that prM cleavage, the collapse of E protein ectodomains onto the virion surface, the large movement of the membrane domains of both E and M, and the release of the pr fragment from the particle render the virus mature and infectious. Our work favors the collapse model of flavivirus maturation warranting further studies of immature flaviviruses to determine the sequence of events and mechanistic details driving flavivirus maturation. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

15. Structure and replication cycle of a virus infecting climate-modulating algaEmiliania huxleyi

Author

Sub Structural Biochemistry, Structural Biochemistry, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., Plevka, Pavel, Sub Structural Biochemistry, Structural Biochemistry, Homola, Miroslav, Büttner, Carina R., Füzik, Tibor, Křepelka, Pavel, Holbová, Radka, Nováček, Jiří, Chaillet, Marten, Förster, Friedrich, Wilson, William H., Schroeder, Declan C., and Plevka, Pavel

Published
2023

22. Virion structure and in vitro genome release mechanism of dicistrovirus Kashmir bee virus

Author

Government of Czech Republic, European Commission, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Universidad Nacional de La Plata, Universidad del País Vasco, Mukhamedova, Liya, Füzik, Tibor, Nováček, Jiří, Hrebík, Dominik, Přidal, Antonín, Marti, Gerardo A., Guérin, Diego M. A., Plevka, Pavel, Government of Czech Republic, European Commission, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Universidad Nacional de La Plata, Universidad del País Vasco, Mukhamedova, Liya, Füzik, Tibor, Nováček, Jiří, Hrebík, Dominik, Přidal, Antonín, Marti, Gerardo A., Guérin, Diego M. A., and Plevka, Pavel

Abstract

Infections of Kashmir bee virus (KBV) are lethal for honeybees and have been associated with colony collapse disorder. KBV and closely related viruses contribute to the ongoing decline in the number of honeybee colonies in North America, Europe, Australia, and other parts of the world. Despite the economic and ecological impact of KBV, its structure and infection process remain unknown. Here we present the structure of the virion of KBV determined to a resolution of 2.8 Å. We show that the exposure of KBV to acidic pH induces a reduction in inter-pentamer contacts within capsids and the reorganization of its RNA genome from a uniform distribution to regions of high and low density. Capsids of KBV crack into pieces at acidic pH, resulting in the formation of open particles lacking pentamers of capsid proteins. The large openings of capsids enable the rapid release of genomes and thus limit the probability of their degradation by RNases. The opening of capsids may be a shared mechanism for the genome release of viruses from the family Dicistroviridae ImportanceThe western honeybee (Apis mellifera) is indispensable for maintaining agricultural productivity as well as the abundance and diversity of wild flowering plants. However, bees suffer from environmental pollution, parasites, and pathogens, including viruses. Outbreaks of virus infections cause the deaths of individual honeybees as well as collapses of whole colonies. Kashmir bee virus has been associated with colony collapse disorder in the US, and no cure of the disease is currently available. Here we report the structure of an infectious particle of Kashmir bee virus and show how its protein capsid opens to release the genome. Our structural characterization of the infection process determined that therapeutic compounds stabilizing contacts between pentamers of capsid proteins could prevent the genome release of the virus.

Published
2021

25. Capsid Structure of Leishmania RNA Virus 1

Author

Procházková, Michaela, primary, Füzik, Tibor, additional, Grybchuk, Danyil, additional, Falginella, Francesco Luca, additional, Podešvová, Lucie, additional, Yurchenko, Vyacheslav, additional, Vácha, Robert, additional, and Plevka, Pavel, additional

Published
2021
Full Text
View/download PDF

26. Capsid opening enables genome release of iflaviruses

Author

Škubník, Karel, primary, Sukeník, Lukáš, additional, Buchta, David, additional, Füzik, Tibor, additional, Procházková, Michaela, additional, Moravcová, Jana, additional, Šmerdová, Lenka, additional, Přidal, Antonín, additional, Vácha, Robert, additional, and Plevka, Pavel, additional

Published
2021
Full Text
View/download PDF

35. Description and Comparative Genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., Novel Macrococci From Human Clinical Material With Virulence Potential and Suspected Uptake of Foreign DNA by Natural Transformation

Author

Mašlaňová, Ivana, primary, Wertheimer, Zuzana, additional, Sedláček, Ivo, additional, Švec, Pavel, additional, Indráková, Adéla, additional, Kovařovic, Vojtěch, additional, Schumann, Peter, additional, Spröer, Cathrin, additional, Králová, Stanislava, additional, Šedo, Ondrej, additional, Krištofová, Lucie, additional, Vrbovská, Veronika, additional, Füzik, Tibor, additional, Petráš, Petr, additional, Zdráhal, Zbyněk, additional, Ružičková, Vladislava, additional, Doškař, Jiří, additional, and Pantuček, Roman, additional

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results