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In order to achieve intelligent real-time target detection in high-speed scenes, a high-speed camera platform is designed based on FPGA of ZYNQ7000 and target detection algorithm is implemented on it. As a result, one prototype of integrated high frame rate real-time target detection system was created. This system connects the high-speed CMOS signal to the FPGA directly, doing data processing and implementing target detection algorithm through native FPGA, and outputs real-time target position sequence eventually. This system performed pipeline operation of background differencing, binarization, and centroid detection on high-speed video stream images by using a pipeline structure of FPGA design. Due to this operation, the system achieved the synchronization of image acquisition and target detection. The experimental results show that this system can achieve real-time target detection and output target position greater than 1 100 f/s at 560×480 resolution, meanwhile, the detection accuracy can be up to 3 pixels.