Search

International audience; Introduction :Escherichia coli représente 62% des entérobactéries sécrétrices de béta-lactamase à spectre étendu (BLSE) en France. Une surveillance génomique a été initiée sur les E. coli sécrétrices de béta-lactamase au CHU de Caen depuis 2012. Au cours de cette étude, deux épidémies d’E. coli sécrétrices de BLSE au sein de services de réanimation néonatale a été particulièrement investiguée.Matériels et méthodes :Entre Juin et Aout 2017, 28 souches d’E. coli sécrétrices de BLSE isolées dans les services de réanimation néonatales des hôpitaux de Cherbourg et de Caen ont été collectées. Au total 94 souches ont été séquencées dans le cadre de la surveillance. Les caractéristiques des bactéries : Sequence-Type (ST), sérotype, gènes de résistance, de virulence et plasmides ont été déterminés in silico grâce à différentes bases de données. Une phylogènie basée sur la recherche de Single Nucleotide Polymorphism (SNP) a été réalisée par mapping sur un génome de référence puis par comparaison à l’ensemble des 3765 génomes d’E. coli ST131 disponibles dans les bases de données.Résultats :Douze patients âgés d’un à cinq mois ont été colonisés ou infectés par une souche d’E. coli sécrétrice de BLSE entre Juin et Aout 2017. Les bactéries ont été isolées à partir de selles (n=22), yeux (n=3), hémocultures (n=2) et tractus respiratoire (n=1). Toutes les souches des services de réanimation appartenaient au clade ST131 O16:H5 Fim 41 sécrétant la béta-lactamase CTX-M-27 confirmant le caractère épidémique de cet évènement au vu des caractéristiques différentes des E. coli circulant au CHU de Caen depuis 2012. De plus, la phylogénie basée sur les SNP indiquait une faible divergence entre ces souches avec un nombre moyen en SNP de 77 alors qu’elles divergeaient des génomes d’E. coli BLSE ST131 non épidémique de plus de 13000 SNP. L’augmentation de la puissance des analyses par ajout de 3765 génomes d’E. coli ST131, a permis (i) de révéler que les 2 épidémies de Cherbourg et Caen étaient indépendantes (900 SNP de différence) et (ii) de démontrer une récente dissémination mondiale du clade E. coli ST131 O16:H5.Conclusion :La béta-lactamase CTX-M-27 dissémine rapidement au sein d’hôpitaux Français via un clade émergent d’E. coli ST131 O16:H5 Fim 41 différent des clades précédemment décrits. Des études phénotypiques doivent être menées afin de comprendre le succès de ce clone.

International audience; Introduction :Les entérobactéries sécrétrices de béta-lactamase à spectre étendu (BLSE) sont responsables d’une augmentation de la morbi-mortalité infectieuse. Cette étude s’est intéressée à la diversité génomique des souches d’E. coli sécrétrices de BLSE au de CHU de Caen sur une période de 5 ans.Matériels et méthodes :Cinquante-trois souches représentant la première indentification d’une BLSE chez des patients uniques, isolées entre 2012 et 2016, à partir d’écouvillonnages rectaux ont été séquencées en génome entier. Les caractéristiques des bactéries : Sequence-Type (ST), sérotype, gènes de résistance et gènes de virulence ont été déterminés in silico grâce aux bases de données.Résultats :Les prélèvements provenaient de 16 différents services, majoritairement les services de médecine, de chirurgie puis de réanimation (40%, 31%, 29%). Le sex ratio H/F était de 0,9. L’âge moyen des patients était de 64 ans ; 55% avaient été hospitalisés dans les 3 mois précédents ; 51% ont reçu une antibiothérapie au cours de leur hospitalisation et 14% avaient voyagé hors de France.Au total, 34 différents ST et 35 sérotypes ont été retrouvés. Les populations E. coli les plus fréquentes étaient les ST131 O25:H4 (n=6), ST10 O89:H9 (n=3) et ST162:O9:H9 (n=2). Onze différentes béta-lactamases ont été détectées, les plus fréquentes étaient : CTX-M-1 (n=18), CTX-M-14 (n=9), CTX-M-15 (n=7). Pour 27 patients (49%), la souche d’E. coli BLSE a été identifiée avant 48h d’hospitalisation, principalement CTX-M-14. Tandis que l’enzyme « nosocomiale » était volontiers CTX-M-15 (délai moyen d’acquisition 19 jours, min/max : 0/66 jours). Enfin, CTX-M-14 et SHV-12 étaient significativement plus retrouvées pour les souches d’E. coli circulantes au sein des services de réanimation (p=0,02, p=0,02) supposant une circulation diffuse de souches épidémiques.Conclusion :Cette étude d’épidémiologie génomique a permis de révéler une grande diversité de populations des souches d’E. coli sécrétrices de BLSE au CHU de Caen depuis 2012. Cela supporte que dans 50% des cas le portage de ces bactéries était probablement d’origine communautaire (détection dans les 48h après l’admission). D’autres souches hospitalières, communautaires et vétérinaires sont en cours de séquençage afin d’augmenter la puissance de l’étude sur leurs attributions et leurs circulations en Normandie qui semblent assez étendues.

National audience

Au printemps 2010, une chenille verte de plusieurs cm de long (jusqu'à 5-6 cm) a envahi plusieurs dizaines d'hectares de vignobles (Luc Sur Orbieu, Cruscades, Ornaisons, Gasparet et Boutenac) En quelques semaines (avril et mai), des densités très élevées de chenilles ont entraîné des dommages pouvant aller jusqu'à 60 à 80 % de perte de récolte dans certaines parcelles. L'espèce responsable de ces dégâts est une noctuelle du genre Xydena, vraisemblablement Xylena exsoleta, même si des adultes sont requis pour une identification certaine. Une présentation de la biologie de cette espèce est donnée dans cet article. De plus, une analyse des causes possibles des pullulations de ce phytophage habituellement inoffensif pour les cultures est réalisée. Enfin, certaines démarches à suivre pour 2011 sont proposées afin d'éviter les pullulations observées en 2010.

International audience; The house mouse (Mus musculus domesticus) and the short-tailed mouse of the eastern Mediterranean area (M. macedonicus) were thought to live sympatrically on Cyprus Island. Recently, a phylogenetic survey has shown that the non-commensal mouse of Cyprus was an unknown sister species of European wild mice. Here, we describe this new species of the genus Mus (Rodentia, Mammalia), namely Mus cypriacus sp. n., based on 19 specimens trapped in the southern part of Cyprus. These animals were first compared to Eurasian species of mice using both molecular genetics (complete D-loop sequences and nuclear gene intron) and cytogenetics to state on its systematic status. Then classical and geometric morphometric analyses on both cranial and dental characters have been performed to compare Mus cypriacus with circum-Mediterranean species and provide diagnositic morphological characters. Genetic data strongly support a sister species relationship of the new species to M. macedonicus, the closest mainland taxon. Morphometric analyses provide satisfying criteria for diagnosis of this species relative to other Mediterranean species. The most obvious phenotypic characteristics are its long tail and the allometric gigantism and shape robustness of its cranial and dental characters compared to other Mediterranean mice. The molecular clock and the history of the murine settlement on Cyprus are congruent and suggest that the common ancestor of M. cypriacus and M. macedonicus arrived on Cyprus during the Middle Pleistocene by a founder event on natural raft. The remoteness of Cyprus through time has prevented introgression from the mainland gene pool, and favoured phenotypic adaptation to competition release, leading to the allopatric speciation of M. cypriacus.

Description of Mus cypriacus sp. n. Mammalia, Rodentia, Muridae, Murinae Genus Mus Linneaus, 1758 Mus cypriacus sp. nov. Holotype Number 2005 ­ 988 in the collection of Vertébrés, Mammifères et Oiseaux, of the Muséum National d’Histoire Naturelle, Paris, France (MNHN). Adult female (skull and body in alcohol), age class 5 (6­8 months) (sensu Lidicker 1966); collected on the 22 nd of April 2004, by Josette Catalan, Annie Orth and Laurent Fabre at Alassa village of Lemesos (Limassol) district of Cyprus (34 ° 46 N, 32 ° 55 EW). Head and body: 84 mm, tail: 72 mm, foot: 18 mm Sampling of soft parts: muscles for DNA analysis and digestive tractus Karyotype (2 N= 40) Paratype 1 Number 2005 ­ 989 in the collection of Vertébrés, Mammifères et Oiseaux, of the Muséum National d’Histoire Naturelle, Paris, France (MNHN). Adult female (skull and body in alcohol), age class 4 (4­6 months) (sensu Lidicker (1966)), collected on the 26 th April 2004 by Jean­Christophe Auffray and François Bonhomme at Pachna (Lemesos district), Cyprus (34 ° 47 N, 32 ° 47 EW) Head and body: 79 mm, tail: 75 mm, foot: 17 mm Sampling of soft parts: liver for DNA analysis and digestive tract. Karyotype (2 N= 40). Paratype 2 Number 2005 ­ 990 in the collection of Vertébrés, Mammifères et Oiseaux, of the Muséum National d’Histoire Naturelle, Paris, France (MNHN); Adult female (skull and body in alcohol), age class 5 (6­8 months) (sensu Lidicker (1966)), collected on the 26 th of April 2004, by Josette Catalan, Annie Orth, Jean­Christophe Auffray, François Bonhomme, Thomas Cucchi, Laurent Fabre and Eleftherios Hadjisterkotis at Pera Pedi (Lemesos district), Cyprus (33 ° 44 N, 32 °04 EW) Head and body: 81 mm, tail: 74 mm, foot: 18 mm Sampling of soft parts: liver for DNA analysis and digestive tractus Karyotype (2 N= 40) Type locality Alassa (34 ° 46 N, 32 ° 55 EW) is located at 322 meters a.s.l. in the Troodos region. The specimens were live­trapped in abandoned cultivation terraces colonised by Thorny Broom (Calycotome villosa), Thorny Gorse (Genista sphacelata), Rock­Rose (Cistus creticus and C. salviifolius), Asphodel (Asphodelus aestivus) and shrubs such as Mastic trees, or Lentisks (Pistacia lentiscus) and grasses (Avena sp. oats). Pachna (34 ° 47 N, 32 ° 47 EW) is located at 655 meters a.s.l. in the Troodos region. The specimens were live­trapped in ancient Babylonian­type terraces and vineyard (Vitis vinifera) with low walls which favour the growth of Rock­roses, Terebinth (Pistacia terebinthus), Almond trees (Prunus dulcis) and some rosaceae nearby, Prickly burnet (Sarcopoterium spinosum) and Mediterranean Hawthorn (Crataegus azarolus). Pera Pedi (33 ° 44 N, 32 °04 EW) is located at 849 meters a.s.l. in the Troodos region. The specimens were live­trapped at the edge of a vineyard mixed with Syrian Pears (Pyrus syriaca), bordering with an abandoned vineyard invaded by Thorny Broom and Thorny Gorse. On the uncultivated terraced border there were Mastic trees, Terebinths and Almond Trees (Prunus dulcis = Amygdalus communis). Other material 1 young adult male (skull and body in alcohol) of the first generation from the breeding colony at Montpellier University in the collection of the Slovene Museum of Natural History, Ljubljana, Slovenia. Sampling of soft parts (liver for DNA analysis and digestive tract is available. 15 skulls (10 males and 5 females) in the collection of the ISE­M (UMR 5554), Université Montpellier 2, Montpellier, France Etymology Latin: “from Cyprus ” Vernacular name: “the Cyprus mouse” Distribution and habitat Among the 15 trapping localities sampled in different biotopes on the southern part of the island, Mus cypriacus was captured in eight of them. If further ecological studies and trapping campaigns are required in order to establish the range of the new species, it is possible to identify some trends in its distribution and habitat. Mus cypriacus was found mainly in the Troodos region between 300 and 900 meters a.s.l. Its habitat comprises abandoned cultivation terraces with vineyard, grassy fields, and bushes such as Mastic trees, Terebinths Thorny Broom and Thorny Gorse. In the altitudinal range of 100­150 m, Mus cypriacus can be syntopic with the house mouse (M. m. domesticus) in forested riverine areas. At low altitudes (less than 100 meters a.s.l.) Mus cypriacus seems absent from areas with strong anthropogenic pressure such as the overexploited agricultural fields of the Mesaoria (central plain of the island), human dwellings and farms, orchards (orange groves) where the house mouse is almost exclusively dominant (Cucchi 2005). Description of external and cranial characters Mus cypriacus (Figure 3) is a typical mouse with clear agouti fur on the back and a whitish belly. The underpants, hands and feet are pinkish. The ears are large and elongated with white hairs behind them. The eyes are large and protruding. The tail is long compared to the head and body length. The mammary formula is 3 + 2 = 10. The skull (Figure 4) is large but typical of the subgenus Mus. To illustrate the general morphology of the skull we have figured the different views of the Holotype and the paratype 1 because the holotype skull is incomplete (dislocation of the interparietal and the right tympanic bulla during measurements). Mus cypriacus presents a shallow and angulated skull with a very robust general appearance. In ventral and dorsal views, the zygomatic arch is very angular, wide and thick and provides a large ocular orbit. The anterior part of the malar process is large. The squamosal process is well developed with a strong embranchment on the skull box. In the dorsal view, the fronto­parietal ridge is angular and salient. The coronal suture is hemi­circular. A crest marks the parietal/interparietal suture. The external occipital crest is also strongly pronounced. In dorsal and ventral views the muscular insertion of the anterior superficial masseter just posterior to the suture of the maxilla and premaxilla is proeminent. In the lateral view, according to the criteria of Marshall & Sage (1981), the suture between the ventral wing of the parietal and the squamosal is simple, regular and gently curved. The post tympanic hook of the squamosal bone is large and projected in a posterior direction. The anterior profile of the zygomatic plate is straight. The anterior part of the first lower molar (m 1) of Mus cypriacus is tetralobed according to the description of Darviche & Orsini (1982) because of a developed and distinct labial anteroconid (tE according to Michaux (1971). Comparisons with the other circum­Mediterranean species Mitochondrial DNA and nuclear genes The trees presented in figure 5 A and 5 B confirm the results reported in Bonhomme et al. (2004), with the difference that Mus spretus has been added as a supplementary external group. In figure 5 A, bootstrap values show a good support for the new species as a sister taxon of the eastern Mediterranean short­tailed mouse M. macedonicus (including its two subspecies M. macedonicus macedonicus and M. macedonicus spretoides which are the closest mainland relatives), not very far after the divergence of Mus spicilegus. In figure 5 B however, the ABPa intron 2 sequences tend to show a higher closeness of cypriacus with spicilegus, although this is loosely supported (54 % bootstrap). Based on the D­loop net % divergence between species (musculus s.lato / spretus = 11.04 %, cypriacus / macedonicus s.lato = 4.17 %, K 2 P model with gamma correction as for the phylogenetic inference), we can estimate a divergence time for Mus cypriacus, using an external calibration point. This point is given by the study of Chevret et al., (2003), who set a divergence of M. spretus and M. musculus s.lato at 1.4 Myr ago, considering a Mus / Rattus divergence at 12 Myrs. Thus the cypriacus / macedonicus ­spretoides divergence time is estimated at: 1.4 x 4.17 / 11.04 = 0.53 Myrs. Additionnally the Øp 53 pseudogene is present in all the cypriacus samples, as in macedonicus­spretoides, spicilegus and domesticus. It is absent in spretus and musculus (Ohtsuka, et al., 1996; Tanooka, et al., 2001). As to the Zfy­ 2 Y chromosome gene, the ten cypriacus males tested showed a musculus pattern as in macedonicus­spretoides, spicilegus and spretus, but unlike domesticus (Tucker, et al., 1989). Chromosomal data All the individuals analysed presented a karyotype composed of 40 acrocentric chromosomes (2 n = 40 ­ NF = 40), which is a diagnostic character of the whole subgenus Mus (Boursot et al. 1993). Chromosomal identification by G­banding revealed that Mus cypriacus presents the standard band pattern of the subgenus Mus described by Cowell (1984). The Y chromosome is as large as the one in Mus m. domesticus from Cyprus, as well as in the other subspecies of M. musculus (M. m. domesticus, M. m. musculus, M. m. castaneus). This large Y chromosome contrasts with the small Ys described for wild European mice Mus macedonicus (Israel: (Ivanitskaya, et al., 1996); Greece and Bulgaria: J. Britton­ Davidian pers. com.), Mus spicilegus (southern USSR: (Bulatova & Kotenkova., 1990); Bulgaria: J. Britton­Davidian pers. com), Mus spretus (Spain and France: J. Britton­Davidian pers. com.), as well as in two Asian mice Mus booduga (Sen & Sharma, 1983) and M. cervicolor (Markvong, et al., 1973). External measurements and cranio­dental morphometry Morphometric traits The range of morphometric variables used by Orsini et al. (1983) is summarized in table 3. The body size range of Mus cypriacus (75­91) falls within the one of all the Mediterranean species but its Tail length and HB/T index mean values (74.15 and 1.10, respectively) fall within the ranges of the house mouse (M. m. domesticus) (69­88 and 0.90­1.20, respectively). Mus cypriacus zygomatic index (0.86) and lower tooth row length (3.39) are slightly larger than that of wild Mediterranean relatives (0.60 ­082 and 3.01­3.19, respectively). Mus cypriacus and M. m. domesticus present the same relative tail size (P: NS) (Table 4). Mus cypriacus shares similar values of ZI with wild species (P: NS) but has a significantly longer tooth row (LTR) (P ***) than all its relatives (Table 4). ANOVA HB F3.866df= 4** Tuckey HSD M. m. domesticus M. m. macedonicus M. m. spretoides M. cypriacus M. spretusM. m. domesticus ­­­ * NS NS NSM. m. macedonicus ­ 6.093 ­­­ NS NS NSM. m. spretoides ­ 2.615 3.478 ­­­ NS NSM. cypriacus ­ 0.870 5.222 1.744 ­­­ NS M. spretus 1.452 7.544 4.067 2.322 ­­­ ANOVA T F51.777df= 4*** Tuckey HSD M. m. domesticus M. m. macedonicus M. m. spretoides M. cypriacus M. spretusM. m. domesticus ­­­ *** *** NS ***M. m. macedonicus ­11.574 ­­­ NS *** ***M. m. spretoides ­15.385 ­ 3.811 ­­­ *** *M. cypriacus ­3.352 8.222 12.033 ­­­ ***M. spretus ­21.452 ­9.878 ­ 6.067 ­ 18.1 ­­­ ANOVA HB/T F46.182df=4*** Tuckey HSD M. m. domesticus M. m. macedonicus M. m. spretoides M. cypriacus M. spretus M. m. domesticus ­­­ * *** NS *** M. m. macedonicus 0.095 ­­­ ** NS *** M. m. spretoides 0.221 0.126 ­­­ *** *** M. cypriacus 0.038 ­0.057 ­0.183 ­­­ *** M. spretus 0.446 0.351 0.226 0.408 ­­­ ANOVA ZI F8.445df=4*** Tuckey HSD M. m. domesticus M. m. macedonicus M. m. spretoides M. cypriacus M. spretus M. m. domesticus ­­­ *** *** *** *** M. m. macedonicus 0.219 ­­­ NS NS NS M. m. spretoides 0.234 0.015 ­­­ NS NS M. cypriacus 0.253 0.033 0.018 ­­­ NS M. spretus 0.193 ­0.027 ­0.042 ­0.6 ­­­ ANOVA LTR F29.268df=4*** Tuckey HSD M. m. domesticus M. m. domesticus ­­­ M. m. macedonicus 0.183 M. m. spretoides 0.149 M. cypriacus 0.384 M. spretus 0.158 ......continued on the next page Geometric morphometrics of cranial and dental characters Sexual dimorphism on size and shape Sexual dimorphism has generally no or little influence on the size and shape of cranial and dental traits in rodent species (Auffray, et al., 1996; Renaud, 2005). Our results are in agreement with these views. Sexual dimorphism does not appear to be sufficient to interfere with the size and shape differentiation of skulls, teeth and mandibles within the different species (Table 5). A test focusing on Mus cypriacus confirmed the absence of significant sexual dimorphism on size and shape of cranial and dental features (Table 6). Therefore, the animals were pooled independently of their sex in the subsequent statistical analyses. ......continued on the next page Interspecific size difference All morphological features (skull, mandible and molars) show differences in size among species (Table 7). Mus cypriacus is significantly larger than its Mediterranean relatives. Interspecific shape difference Cranial characters Significant interspecific morphological differentiation was found for both skulls (dorsal and ventral views, MANOVA on Procrustes residuals: P P The combination of shape (PC 1) and size (CS) information allows a clear separation of Mus cypriacus whereas the skull configurations of the other species display an important overlap (fig. 6 AB). On the dorsal view, Mus cypriacus tends to segregate towards positive values on the shape axis, being opposed to domesticus. The main difference lies in the squamosal process (LMs 9, 10, 18) which is much more developed in Mus cypriacus than in the other species (fig. 6 A). Mus cypriacus is also characterised by a wide and anteriorly displaced zygomatic plate (LM 11), a wide anterior part of the malar process (LMs 14, 15), a longer nasal bone and a narrower parietal on the median axis (LMs 3, 4). In the ventral view, cypriacus specimens are tightly clustered on the shape axis towards negative PC 1 values. There is an overlap, however, with the large range of variation of spretus. In the ventral view (Fig. 6 B), Mus cypriacus differs mainly because of its relatively longer upper tooth row (LMs 9,10) and nasal bone (LMs 1,2) which enlarge the anterior part of its skull. The posterior part of the skull of Mus cypriacus shows a reduction of the occipital foramen (LMs 7,8), and a posteriorly displaced external acoustic meatus (LMs 16,17,19). The pattern of differentiation observed for the mandible (fig. 7 AB) shows that Mus cypriacus emerges as a well separate group whereas the other species, except domesticus segregating along CA 2, share similar outline shapes leading to an important overlap on the canonical plane. For mandibles the differences are difficult to describe, but we can observe that the divergence of Mus cypriacus lies mainly on the height of the corpus as well as on the convexity of the tooth row edge, probably because the molar alveolus bears larger teeth (See table 4). The angle of the incisor alveolus and the development and position of the coronoid process are also probably involved in the divergence of Mus cypriacus from other Mediterranean species. Generally, Mus cypriacus displays a more robust mandible than other Mediterranean species. Dental characters Similarly, molars display highly significant interspecific divergences (MANOVA on Fourier coefficients: P The pattern of differentiation of the first lower molar displayed on the first canonical plane (Fig. 7 C), represents up to 81 % of the among­group variance, and shows that the different species are distinguished from one another without overlap. Mus spretus occupies a central position whereas domesticus segregates towards positive CA 1 values. Both macedonicus subspecies show a similar range of negative scores along CA 1. These two taxa segregate along CA 2, however. This axis also isolates cypriacus, characterised by negative scores along CA 2. The mean reconstructed outline corresponding to each taxon allows us to interpret this general pattern in terms of difference in tooth shape. Mus cypriacus is characterised by a wide molar with a well­developed lingual anteroconid (tD) that causes a general squared shape to the m 1. .................................to be continued The pattern of differentiation of the first upper molar shape (Fig. 7 D) is slightly different. Mus spretus is once again in a rather central position but Mus cypriacus is separated from the other taxa more clearly than for the m 1, clustering towards positive values of CA 1. The second axis clearly separates M. m. domesticus (positive CA 2 values), from M. macedonicus macedonicus. According to mean outlines, the M 1 of Mus cypriacus is wider and its postero­labial part is more convex, apparently because of a more developed t 8. Furthermore, Mus cypriacus mainly differs from relative Mediterranean species by a more developed t 1 and t 6. Allometric variation A linear regression of the synthetic shape variable (PC 1 for skull, CA 1 for the molars and mandibles) on size (Log­transformed CS for skull, Ha 1 area for mandibles and molars) has been performed in order to investigate possible patterns of allometric variation. The shape of the skull in both dorsal (n = 57, r = 0.548, r 2 = 0.301, P n = 57, r = 0.464, r 2 = 0.183, P

Salmonella genomic island 1 (SGI1) harbors an antibiotic resistance gene cluster and was previously identified in the multidrug-resistant Salmonella enterica serovars Typhimurium DT104, Agona, Paratyphi B, and Albany. This antibiotic resistance gene cluster is a complex class 1 integron and most often confers resistance to ampicillin (Ap), chloramphenicol (Cm)/florfenicol (Ff), streptomycin (Sm)/spectinomycin (Sp), sulfonamides (Su), and tetracycline (Tc) (ApCmFfSmSpSuTc profile). Recently, variant SGI1 antibiotic resistance gene clusters conferring different antibiotic resistance profiles have been identified in several S. enterica serovars and were classified as SGI1-A to -G. We identified a new variant SGI1 antibiotic resistance gene cluster in two multidrug-resistant S. enterica serovar Newport strains isolated from humans in France. In these strains, the Sm/Sp resistance gene cassette aadA2 inserted at the first attI1 site was replaced by two other aminoglycoside resistance gene cassettes. The first one contains a new resistance gene encoding an AAC(3)-I aminoglycoside 3-N-acetyltransferase that confers resistance to gentamicin (Gm) and sisomicin (Sc). This gene has been named aac(3)-Id. The second one harbors the Sm/Sp resistance gene aadA7. This gene cassette replacement in the SGI1 complex integron of serovar Newport strains constitutes a new variant SGI1 antibiotic resistance gene cluster named SGI1-H. The occurrence of SGI1 in different S. enterica serovars, now including serovar Newport, strengthens the hypothesis of horizontal transfer of SGI1.