1. A Fluorescent Alternative to the Synthetic Strigolactone GR24
- Author
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Rik Van Deun, Danny Geelen, François-Didier Boyer, Christian V. Stevens, Amanda Rasmussen, Cedrick Matthys, Sofie Goormachtig, Thomas S. A. Heugebaert, Institut de Chimie des Substances Naturelles (ICSN), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), Faculty of Bioscience Engineering, Ghent University, Universiteit Gent = Ghent University [Belgium] (UGENT), Department of Plant Production [Ghent], Faculty of Bioscience Engineering [Ghent], Universiteit Gent = Ghent University [Belgium] (UGENT)-Universiteit Gent = Ghent University [Belgium] (UGENT), FP7 Marie Curie International Incoming Fellowships, Hercules Foundation [AUGE/09/024], Research Foundation-Flanders [0081.10N], and Ghent University [01N01010]
- Subjects
0106 biological sciences ,Arabidopsis ,Strigolactone ,Germination ,strigolactones ,Plant Science ,Isoindoles ,Plant Roots ,01 natural sciences ,Lactones ,03 medical and health sciences ,4-Butyrolactone ,Gene Expression Regulation, Plant ,In vivo ,branching ,Botany ,adventitious rooting ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,biology ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,Arabidopsis Proteins ,Orobanche ,Biological activity ,biology.organism_classification ,In vitro ,Spectrometry, Fluorescence ,fluorescent markers ,Biochemistry ,parasitic weed seed germination ,Shoot ,Signal transduction ,Carrier Proteins ,Plant Shoots ,010606 plant biology & botany - Abstract
International audience; Strigolactones have recently been implicated in both above- and below-ground developmental pathways in higher plants. To facilitate the molecular and chemical properties of strigolactones in vitro and in vivo, we have developed a fluorescent strigolactone molecule, CISA-1, synthesized via a novel method which was robust, high-yielding, and used simple starting materials. We demonstrate that CISA-1 has a broad range of known strigolactone activities and further report on an adventitious rooting assay in Arabidopsis which is a highly sensitive and rapid method for testing biological activity of strigolactone analogs. In this rooting assay and the widely used Orobanche germination assay, CISA-1 showed stronger biological activity than the commonly tested GR24. CISA-1 and GR24 were equally effective at inhibiting branching in Arabidopsis inflorescence stems. In both the branching and adventitious rooting assay, we also demonstrated that CISA-1 activity is dependent on the max strigolactone signaling pathway. In water methanol solutions, CISA-1 was about threefold more stable than GR24, which may contribute to the increased activity observed in the various biological tests.
- Published
- 2013
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