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4. Comparative Evaluation of Mathematical Model and In Vivo Study of Calcium Phosphate Bone Grafts.

Author

Shlykov, Mikhail A., Smirnova, Polina V., Senotov, Anatoliy S., Teterina, Anastasia Yu., Minaychev, Vladislav V., Smirnov, Igor V., Novikov, Roman A., Marchenko, Ekaterina I., Salynkin, Pavel S., Komlev, Vladimir S., Fadeev, Roman S., and Fadeeva, Irina S.

Subjects
CALCIUM phosphate, CHEMICAL kinetics, LABORATORY rats, BONE grafting, SURFACES (Technology)
Abstract

One of the key factors of the interaction 'osteoplastic material—organism' is the state of the implant surface. Taking into account the fact that the equilibrium in regeneration conditions is reached only after the reparative histogenesis process is completed, the implant surface is constantly modified. This work is devoted to the numerical description of the dynamic bilateral material–medium interaction under close to physiological conditions, as well as to the assessment of the comparability of the model with in vitro and in vivo experimental results. The semi-empirical model obtained on the basis of chemical kinetics allows us to describe numerically the processes occurring in the in vitro systems and extrapolates well to assess the behavior of dicalcium phosphate dihydrate (DCPD) material under conditions of ectopic (subcutaneous) implantation in Wistar rats. It is shown that an experiment conducted using a perfusion–diffusion bioreactor in a cell culture medium with the addition of fetal bovine serum (FBS) allows for achieving morphologically and chemically identical changes in the surface of the material in comparison with the real organism. This fact opens up wide possibilities for the creation of an analog of a 'laboratory-on-a-chip' and the transition from classical in vivo models to more controlled and mathematically based in vitro systems. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Low-Temperature Calcium Phosphate Ceramics Can Modulate Monocytes and Macrophages Inflammatory Response In Vitro

Author

Minaychev, Vladislav V., primary, Smirnova, Polina V., additional, Kobyakova, Margarita I., additional, Teterina, Anastasia Yu., additional, Smirnov, Igor V., additional, Skirda, Vladimir D., additional, Alexandrov, Artem S., additional, Gafurov, Marat R., additional, Shlykov, Mikhail A., additional, Pyatina, Kira V., additional, Senotov, Anatoliy S., additional, Salynkin, Pavel S., additional, Fadeev, Roman S., additional, Komlev, Vladimir S., additional, and Fadeeva, Irina S., additional

Published
2024
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7. Composite Remineralization of Bone-Collagen Matrices by Low-Temperature Ceramics and Serum Albumin: A New Approach to the Creation of Highly Effective Osteoplastic Materials

Author

Minaychev, Vladislav V., primary, Teterina, Anastasia Yu., additional, Smirnova, Polina V., additional, Menshikh, Ksenia A., additional, Senotov, Anatoliy S., additional, Kobyakova, Margarita I., additional, Smirnov, Igor V., additional, Pyatina, Kira V., additional, Krasnov, Kirill S., additional, Fadeev, Roman S., additional, Komlev, Vladimir S., additional, and Fadeeva, Irina S., additional

Published
2024
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8. Optimizing Antimicrobial Peptide Design: Integration of Cell-Penetrating Peptides, Amyloidogenic Fragments, and Amino Acid Residue Modifications.

Author

Kravchenko, Sergey V., Domnin, Pavel A., Grishin, Sergei Y., Zakhareva, Alena P., Zakharova, Anastasiia A., Mustaeva, Leila G., Gorbunova, Elena Y., Kobyakova, Margarita I., Surin, Alexey K., Poshvina, Darya V., Fadeev, Roman S., Azev, Viacheslav N., Ostroumova, Olga S., Ermolaeva, Svetlana A., and Galzitskaya, Oxana V.

Subjects
ANTIMICROBIAL peptides, CELL-penetrating peptides, AMINO acid residues, PEPTIDE antibiotics, PEPTIDES, ESCHERICHIA coli, AMINO acids
Abstract

The escalating threat of multidrug-resistant pathogens necessitates innovative approaches to combat infectious diseases. In this study, we examined peptides R23FS*, V31KS*, and R44KS*, which were engineered to include an amyloidogenic fragment sourced from the S1 protein of S. aureus, along with one or two cell-penetrating peptide (CPP) components. We assessed the antimicrobial efficacy of these peptides in a liquid medium against various strains of both Gram-positive bacteria, including S. aureus (209P and 129B strains), MRSA (SA 180 and ATCC 43300 strains), and B. cereus (strain IP 5832), and Gram-negative bacteria such as P. aeruginosa (ATCC 28753 and 2943 strains) and E. coli (MG1655 and K12 strains). Peptides R23FS*, V31KS*, and R44KS* exhibited antimicrobial activity comparable to gentamicin and meropenem against all tested bacteria at concentrations ranging from 24 to 48 μM. The peptides showed a stronger antimicrobial effect against B. cereus. Notably, peptide R44KS* displayed high efficacy compared to peptides R23FS* and V31KS*, particularly evident at lower concentrations, resulting in significant inhibition of bacterial growth. Furthermore, modified peptides V31KS* and R44KS* demonstrated enhanced inhibitory effects on bacterial growth across different strains compared to their unmodified counterparts V31KS and R44KS. These results highlight the potential of integrating cell-penetrating peptides, amyloidogenic fragments, and amino acid residue modifications to advance the innovation in the field of antimicrobial peptides, thereby increasing their effectiveness against a broad spectrum of pathogens. [ABSTRACT FROM AUTHOR]

Published
2024
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9. Enhancing the Antimicrobial Properties of Peptides through Cell-Penetrating Peptide Conjugation: A Comprehensive Assessment

Author

Kravchenko, Sergey V., primary, Domnin, Pavel A., additional, Grishin, Sergei Y., additional, Vershinin, Nikita A., additional, Gurina, Elena V., additional, Zakharova, Anastasiia A., additional, Azev, Viacheslav N., additional, Mustaeva, Leila G., additional, Gorbunova, Elena Y., additional, Kobyakova, Margarita I., additional, Surin, Alexey K., additional, Fadeev, Roman S., additional, Ostroumova, Olga S., additional, Ermolaeva, Svetlana A., additional, and Galzitskaya, Oxana V., additional

Published
2023
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11. Composite Remineralization of Bone-Collagen Matrices: A New Approach to the Creation of Highly Effective Osteoplastic Materials

Author

Fadeeva, Irina S., primary, Minaychev, Vladislav V., additional, Teterina, Anastasia Yu., additional, Smirnova, Polina V., additional, Menshikh, Ksenia A., additional, Senotov, Anatoliy S., additional, Kobyakova, Margarita I., additional, Lomovskaya, Yana V., additional, Smirnov, Igor V., additional, Krasnova, Olga A., additional, Zvyagina, Alyona I., additional, Shlykov, Mikhail A., additional, Pyatina, Kira V., additional, Krasnov, Kirill S., additional, Fadeev, Roman S., additional, Akatov, Vladimir S., additional, and Komlev, Vladimir S., additional

Published
2023
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13. Improving the Stability and Effectiveness of Immunotropic Squalene Nanoemulsion by Adding Turpentine Oil

Author

Krasnova, Olga A., primary, Minaychev, Vladislav V., additional, Akatov, Vladimir S., additional, Fadeev, Roman S., additional, Senotov, Anatoly S., additional, Kobyakova, Margarita I., additional, Lomovskaya, Yana V., additional, Lomovskiy, Alexey I., additional, Zvyagina, Alyona I., additional, Krasnov, Kirill S., additional, Shatalin, Yuriy V., additional, Penkov, Nikita V., additional, Zhalimov, Vitaly K., additional, Molchanov, Maxim V., additional, Palikova, Yuliya A., additional, Murashev, Arkady N., additional, Maevsky, Eugeny I., additional, and Fadeeva, Irina S., additional

Published
2023
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14. Injectable Hydrated Calcium Phosphate Bone-like Paste: Synthesis, In Vitro, and In Vivo Biocompatibility Assessment

Author

Teterina, Anastasia Yu., primary, Minaychev, Vladislav V., additional, Smirnova, Polina V., additional, Kobiakova, Margarita I., additional, Smirnov, Igor V., additional, Fadeev, Roman S., additional, Egorov, Alexey A., additional, Ashmarin, Artem A., additional, Pyatina, Kira V., additional, Senotov, Anatoliy S., additional, Fadeeva, Irina S., additional, and Komlev, Vladimir S., additional

Published
2023
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15. Biomimetic Remineralized Three-Dimensional Collagen Bone Matrices with an Enhanced Osteostimulating Effect

Author

Fadeeva, Irina S., primary, Teterina, Anastasia Yu., additional, Minaychev, Vladislav V., additional, Senotov, Anatoliy S., additional, Smirnov, Igor V., additional, Fadeev, Roman S., additional, Smirnova, Polina V., additional, Menukhov, Vladislav O., additional, Lomovskaya, Yana V., additional, Akatov, Vladimir S., additional, Barinov, Sergey M., additional, and Komlev, Vladimir S., additional

Published
2023
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18. Multiple Antimicrobial Effects of Hybrid Peptides Synthesized Based on the Sequence of Ribosomal S1 Protein from Staphylococcus aureus

Author

Kravchenko, Sergey V., primary, Domnin, Pavel A., additional, Grishin, Sergei Y., additional, Panfilov, Alexander V., additional, Azev, Viacheslav N., additional, Mustaeva, Leila G., additional, Gorbunova, Elena Y., additional, Kobyakova, Margarita I., additional, Surin, Alexey K., additional, Glyakina, Anna V., additional, Fadeev, Roman S., additional, Ermolaeva, Svetlana A., additional, and Galzitskaya, Oxana V., additional

Published
2022
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19. Octacalcium Phosphate for Bone Tissue Engineering: Synthesis, Modification, and In Vitro Biocompatibility Assessment

Author

Teterina, Anastasia Yu., primary, Smirnov, Igor V., additional, Fadeeva, Irina S., additional, Fadeev, Roman S., additional, Smirnova, Polina V., additional, Minaychev, Vladislav V., additional, Kobyakova, Margarita I., additional, Fedotov, Aleksandr Yu., additional, Barinov, Sergey M., additional, and Komlev, Vladimir S., additional

Published
2021
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20. Is It Possible to Create Antimicrobial Peptides Based on the Amyloidogenic Sequence of Ribosomal S1 Protein of P. aeruginosa?

Author

Grishin, Sergei Y., primary, Domnin, Pavel A., additional, Kravchenko, Sergey V., additional, Azev, Viacheslav N., additional, Mustaeva, Leila G., additional, Gorbunova, Elena Y., additional, Kobyakova, Margarita I., additional, Surin, Alexey K., additional, Makarova, Maria A., additional, Kurpe, Stanislav R., additional, Fadeev, Roman S., additional, Vasilchenko, Alexey S., additional, Firstova, Victoria V., additional, Ermolaeva, Svetlana A., additional, and Galzitskaya, Oxana V., additional

Published
2021
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23. Myosin Binding Protein-C Forms Amyloid-Like Aggregates In Vitro

Author

Bobyleva, Liya G., primary, Shumeyko, Sergey A., additional, Yakupova, Elmira I., additional, Surin, Alexey K., additional, Galzitskaya, Oxana V., additional, Kihara, Hiroshi, additional, Timchenko, Alexander A., additional, Timchenko, Maria A., additional, Penkov, Nikita V., additional, Nikulin, Alexey D., additional, Suvorina, Mariya Yu., additional, Molochkov, Nikolay V., additional, Lobanov, Mikhail Yu., additional, Fadeev, Roman S., additional, Vikhlyantsev, Ivan M., additional, and Bobylev, Alexander G., additional

Published
2021
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25. Who is Mr. HAMLET? Interaction of human [alpha]-lactalbumin with monomeric oleic acid

Author

Knyazeva, Ekaterina L., Grishchenko, Valery M., Fadeev, Roman S., Akatov, Vladimir S., Permyakov, Sergei E., and Permyakov, Eugene A.

Subjects
Cell death -- Research, Milk proteins -- Structure, Milk proteins -- Properties, Milk proteins -- Physiological aspects, Milk proteins -- Research, Oleic acid -- Physiological aspects, Oleic acid -- Research, Biological sciences, Chemistry
Abstract

An oleic acid (OA)-pretreated ion-exchange column (HAMLET) that triggers several cell death pathways in various tumor cells is used to prepare a specific state of the human milk [Ca.sup.2+] binding protein [alpha]-lactalbumin (hLA) complexed with OA. The findings indicated that the simple mixing of [alpha]-lactalbumin and oleic acid might result in formation of the complex possessing cytotoxic activity with respect to tumor cells.

Published
2008

26. Smooth muscle titin forms in vitro amyloid aggregates

Author

Bobylev, Alexandr G., primary, Galzitskaya, Oxana V., additional, Fadeev, Roman S., additional, Bobyleva, Liya G., additional, Yurshenas, Darya A., additional, Molochkov, Nikolay V., additional, Dovidchenko, Nikita V., additional, Selivanova, Olga M., additional, Penkov, Nikita V., additional, Podlubnaya, Zoya A., additional, and Vikhlyantsev, Ivan M., additional

Published
2016
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30. Antimicrobial and Amyloidogenic Activity of Peptides Synthesized on the Basis of the Ribosomal S1 Protein from Thermus Thermophilus.

Author

Kurpe, Stanislav R., Grishin, Sergei Yu., Surin, Alexey K., Selivanova, Olga M., Fadeev, Roman S., Dzhus, Ylyana F., Gorbunova, Elena Yu., Mustaeva, Leila G., Azev, Vyacheslav N., and Galzitskaya, Oxana V.

Subjects
PEPTIDE antibiotics, RIBOSOMAL proteins, THERMUS thermophilus, PEPTIDES, BACTERIAL proteins, NUCLEIC acids
Abstract

Controlling the aggregation of vital bacterial proteins could be one of the new research directions and form the basis for the search and development of antibacterial drugs with targeted action. Such approach may be considered as an alternative one to antibiotics. Amyloidogenic regions can, like antibacterial peptides, interact with the "parent" protein, for example, ribosomal S1 protein (specific only for bacteria), and interfere with its functioning. The aim of the work was to search for peptides based on the ribosomal S1 protein from T. thermophilus, exhibiting both aggregation and antibacterial properties. The biological system of the response of Gram-negative bacteria T. thermophilus to the action of peptides was characterized. Among the seven studied peptides, designed based on the S1 protein sequence, the R23I (modified by the addition of HIV transcription factor fragment for bacterial cell penetration), R23T (modified), and V10I (unmodified) peptides have biological activity that inhibits the growth of T. thermophilus cells, that is, they have antimicrobial activity. But, only the R23I peptide had the most pronounced activity comparable with the commercial antibiotics. We have compared the proteome of peptide-treated and intact T. thermophilus cells. These important data indicate a decrease in the level of energy metabolism and anabolic processes, including the processes of biosynthesis of proteins and nucleic acids. Under the action of 20 and 50 μg/mL R23I, a decrease in the number of proteins in T. thermophilus cells was observed and S1 ribosomal protein was absent. The obtained results are important for understanding the mechanism of amyloidogenic peptides with antimicrobial activity and can be used to develop new and improved analogues. [ABSTRACT FROM AUTHOR]

Published
2020
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