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Biogenic emissions affect the urban air quality as they are ozone and secondary organic aerosol (SOA) precursors and should be taken into account when applying photochemical pollution models. The present study presents an estimation of the magnitude of non-methane volatile organic compounds (BNMVOCs) emitted by vegetation over Greece. The methodology is based on computation developed with the aid of a Geographic Information System (GIS) and theoretical equations in order to produce an emission inventory on a 6 × 6 km2 spatial resolution, in a temporal resolution of 1 h covering one year (2016). For this purpose, a variety of input data was used: updated satellite land-use data, land-use specific emission potentials, foliar biomass densities, temperature, and solar radiation data. Hourly, daily, and annual isoprene, monoterpenes, and other volatile organic compounds (OVOCs) were estimated. In the area under study, the annual biogenic emissions were estimated up to 472 kt, consisting of 46.6% isoprene, 28% monoterpenes, and 25.4% OVOCs. Results delineate an annual cycle with increasing values from March to April, while maximum emissions were observed from May to September, followed by a decrease from October to January. [ABSTRACT FROM AUTHOR]