Search

Your search keyword '"Fan, Ruizhi"' showing total 25 results
Start Over Author "Fan, Ruizhi"
25 results on '"Fan, Ruizhi"'

13. The m6A modification mediated-lncRNA POU6F2-AS1 reprograms fatty acid metabolism and facilitates the growth of colorectal cancer via upregulation of FASN.

Author

Jiang, Tao, Qi, Junwen, Xue, Zhenyu, Liu, Bowen, Liu, Jianquan, Hu, Qihang, Li, Yuqiu, Ren, Jing, Song, Hu, Xu, Yixin, Xu, Teng, Fan, Ruizhi, and Song, Jun

Subjects
LIQUID chromatography-mass spectrometry, COLORECTAL cancer, FATTY acids, TUMOR growth, STAINS & staining (Microscopy)
Abstract

Background: Long noncoding RNAs (lncRNAs) have emerged as key players in tumorigenesis and tumour progression. However, the biological functions and potential mechanisms of lncRNAs in colorectal cancer (CRC) are unclear. Methods: The novel lncRNA POU6F2-AS1 was identified through bioinformatics analysis, and its expression in CRC patients was verified via qRT–PCR and FISH. In vitro and in vivo experiments, such as BODIPY staining, Oil Red O staining, triglyceride (TAG) assays, and liquid chromatography mass spectrometry (LC-MS) were subsequently performed with CRC specimens and cells to determine the clinical significance, and functional roles of POU6F2-AS1. Biotinylated RNA pull-down, RIP, Me-RIP, ChIP, and patient-derived organoid (PDO) culture assays were performed to confirm the underlying mechanism of POU6F2-AS1. Results: The lncRNA POU6F2-AS1 is markedly upregulated in CRC and associated with adverse clinicopathological features and poor overall survival in CRC patients. Functionally, POU6F2-AS1 promotes the growth and lipogenesis of CRC cells both in vitro and in vivo. Mechanistically, METTL3-induced m6A modification is involved in the upregulation of POU6F2-AS1. Furthermore, upregulated POU6F2-AS1 could tether YBX1 to the FASN promoter to induce transcriptional activation, thus facilitating the growth and lipogenesis of CRC cells. Conclusions: Our data revealed that the upregulation of POU6F2-AS1 plays a critical role in CRC fatty acid metabolism and might provide a novel promising biomarker and therapeutic target for CRC. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

14. List of contributors

Author

Abd Elhaleem, Maha B., Abdalnoor, Osman A.M., Abdel-Hady, E.E., Adekoya, Mathew Adefusika, Adelani, Samson Oluwagbenga, Ahmad, Azfaralariff, Ahmed, Firoz, Ahmed, Mohammad Boshir, Ahmed, Mohammad Tofayel, Ahmed, Mutasim Abdalla, Aladejebi, Oluwatosin Adekunle, Ali, Isra H., Alom, Jahangir, Alpár, Tibor, Alshrif, Mohammed Tajaldin M., Aniagor, Chukwunonso O., Awuzie, Solomon, Aşık, Müge, Bakri, Mihad Mahmoud, Bin Alam, Shekh Rafin, Bölgen, Nimet, Borges, Jonatas Emmanuel, C., Rajesh, Cai, Yingjie, Chattopadhyay, Dipankar, Chellan, Sasila, Chen, Ruey Shan, Chowdhury, Tonny, Cobra, Khadijatul, Deepa, Simon, Duza, Syeda Sharmin, Ejimofor, Marcel I., Elagib, Tienah H.H., Elango, Jeevithan, El-Raheem, Hany Abd, Erramuspe, Iris Beatriz Vega, Esmaeili, Akbar, Fan, Ruizhi, Farghali, Ahmed, Florea, Ciprian Victor, Galvão, Alessandro Cazonatto, Gan, Sinyee, Guang, Jianye, Habib, Md. Ahsan, Hafez, Sarah H.M., Hasan, K.M. Faridul, Hassan, Elwathig. A.M., Hassan, Md. Sahid, Hassan, Nur Adilah Abu, Hossen, Amjad, Inam, Md. Nafis, Inobeme, Abel, Inobeme, Jonathan, Islam, Md. Hasibul, Islam, Md. Mazharul, Islam, Md. Monirul, Islam, Md. Shahinoor, Jahid, Md. Anwar, Joseph, Kuruvilla, Karatutlu, Ali, Kim, Seong-Cheol, Kumar, Sazal, Lee, Seng Hua, Lin, Ming, Liu, Zigeng, Lopes, Toni Jefferson, López-Maldonado, Eduardo Alberto, Lu, Mengdi, Mahmoud, Rehab, Marjuban, Shaik Merkatur Hakim, Mathew, John Tsado, Matsumura, Kyoya, Menkiti, Matthew C., Mesacasa, Andréia, Mohamed, Hamdy F.M., Mondal, Md. Ibrahim H., Mutlay, Zehra Gizem, Naddeo, Vincenzo, Nitai, Ashis Sutradhar, Norrrahim, Mohd Nor Faiz, Nurruzaman, Md., Oladele, Isiaka Oluwole, Olanrewaju, Olajesu Favor, Omali, Paul, Onaş, Andra Mihaela, Onuh, Linus Nnabuike, Orasugh, Jonathan Tersur, Ortaç, Bülend, Otsuka, Hidenori, Padzil, Farah Nadia Mohammad, Pandele, Andreea Madalina, Patri, Manoranjan, Pertuzatti, Paula Becker, Pervez, Md. Nahid, Rahman, Md. Aminur, Rahman, Md. Hasinur, Rahman, Md. Saifur, Raicopol, Matei D., Ray, Suprakas Sinha, Robazza, Weber da Silva, Roy, Hridoy, Roy, Sukumar, Saha, Siddhartha Sankar, Sakım, Burcu, Samui, Asit Baran, Sfredo, Marilia Assunta, Srinivasan, Sangavi, Stylios, George K., Suwaileh, Wafa, Takebira, Umme Magreba, Talukder, Md. Eman, Teshima, Ryota, Valapa, Ravi Babu, Venkatesan, Raja, Vijayaraghavan, Renuka, Wang, Chen, Wang, Fang, Wu, Wenhui, Youssef, Ahmed F.A., Yıldırım, Elif Yapar, and Zaher, Amal

Published
2025
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results