Search

Your search keyword '"Fan, Wenlei"' showing total 50 results
Start Over Author "Fan, Wenlei"
50 results on '"Fan, Wenlei"'

1. Widely Targeted Metabolomics Revealed the Dynamic Changes of Metabolites during the Formation of Goose Fatty Liver

Author

MA Qiuxia, WANG Baowei, ZHANG Ming’ai, JIA Yiming, SUN Liqian, ZHANG Zihan, WANG Siyi, WANG Yue, FU Yingying, KONG Min, FAN Wenlei

Subjects
goose fatty liver, widely targeted metabolomics, different overfeeding periods, differential metabolites, Food processing and manufacture, TP368-456
Abstract

To understand the composition and dynamic changes of metabolites during the formation of goose fatty liver, the metabolite profiles of goose liver at three overfeeding stages were analyzed using widely targeted metabolomics. Three 70-day-old Landes geese with similar body conditions from the same batch were selected randomly for slaughter at the early (day 7), middle (day 16) and late (day 25) overfeeding stages, separately. The tip of the larger liver lobe was collected for widely targeted metabolomic analysis. The results showed that: (1) a total of 1 153 metabolites belonging to 19 classes including amino acids, organic acids, nucleotides and lipids were detected in the liver of geese at the three overfeeding stages; (2) principal component analysis (PCA) showed significant differences in the metabolic profiles of goose liver at the three stages, and identified 142 and 92 differential metabolites at the early versus middle stage, and the middle versus late stage, respectively, the major ones being amino acids and their derivatives, as well as organic acids and their derivatives; and (3) Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathway analysis indicated that the pathways involved in fatty acid biosynthesis, vitamin B6 metabolism, linoleic acid metabolism, lysine degradation, arginine biosynthesis, arachidonic acid metabolism and amino acid biosynthesis changed significantly during the formation of goose fatty liver. This study found that most of the differential metabolites were involved in fatty acid synthesis during goose fatty liver formation. Moreover, the contents of transport-related metabolites showed a continuous increasing trend. Findings in this study will not only enrich the theoretical knowledge of poultry liver metabolism, but also provide a theoretical basis for the precise nutritional regulation and efficient production of high-quality goose fatty liver.

Published
2024
Full Text
View/download PDF

15. Genetic fine‐mapping reveals single nucleotide polymorphism mutations in the MC1R regulatory region associated with duck melanism

Author

Liu, Hehe, primary, Xi, Yang, additional, Tang, Qian, additional, Qi, Jingjing, additional, Zhou, Zhengkui, additional, Guo, Zhanbao, additional, Fan, Wenlei, additional, Hu, Jian, additional, Xu, Yaxi, additional, Liang, Suyun, additional, Xie, Ming, additional, Tang, Jing, additional, Zhang, Yunsheng, additional, and Hou, Shuisheng, additional

Published
2023
Full Text
View/download PDF

16. Metabolome‐Based Genome‐Wide Association Study of Duck Meat Leads to Novel Genetic and Biochemical Insights

Author

Liu, Dapeng, primary, Zhang, He, additional, Yang, Youyou, additional, Liu, Tong, additional, Guo, Zhanbao, additional, Fan, Wenlei, additional, Wang, Zhen, additional, Yang, Xinting, additional, Zhang, Bo, additional, Liu, Hongfei, additional, Tang, Hehe, additional, Yu, Daxin, additional, Yu, Simeng, additional, Gai, Kai, additional, Mou, Qiming, additional, Cao, Junting, additional, Hu, Jian, additional, Tang, Jing, additional, Hou, Shuisheng, additional, and Zhou, Zhengkui, additional

Published
2023
Full Text
View/download PDF

19. Resequencing of a Pekin duck breeding population provides insights into the genomic response to short-term artificial selection

Author

Yu, Simeng, primary, Liu, Zihua, additional, Li, Ming, additional, Zhou, Dongke, additional, Hua, Ping, additional, Cheng, Hong, additional, Fan, Wenlei, additional, Xu, Yaxi, additional, Liu, Dapeng, additional, Liang, Suyun, additional, Zhang, Yunsheng, additional, Xie, Ming, additional, Tang, Jing, additional, Jiang, Yu, additional, Hou, Shuisheng, additional, and Zhou, Zhengkui, additional

Published
2023
Full Text
View/download PDF

26. Strategies to improve genomic predictions for 35 duck carcass traits in an F2 population.

Author

Cai, Wentao, Hu, Jian, Fan, Wenlei, Xu, Yaxi, Tang, Jing, Xie, Ming, Zhang, Yunsheng, Guo, Zhanbao, Zhou, Zhengkui, and Hou, Shuisheng

Subjects
RACTOPAMINE, DUCKS, ANIMAL breeding, ANIMAL breeds, FORECASTING, MEAT quality
Abstract

Background: Carcass traits are crucial for broiler ducks, but carcass traits can only be measured postmortem. Genomic selection (GS) is an effective approach in animal breeding to improve selection and reduce costs. However, the performance of genomic prediction in duck carcass traits remains largely unknown. Results: In this study, we estimated the genetic parameters, performed GS using different models and marker densities, and compared the estimation performance between GS and conventional BLUP on 35 carcass traits in an F2 population of ducks. Most of the cut weight traits and intestine length traits were estimated to be high and moderate heritabilities, respectively, while the heritabilities of percentage slaughter traits were dynamic. The reliability of genome prediction using GBLUP increased by an average of 0.06 compared to the conventional BLUP method. The Permutation studies revealed that 50K markers had achieved ideal prediction reliability, while 3K markers still achieved 90.7% predictive capability would further reduce the cost for duck carcass traits. The genomic relationship matrix normalized by our true variance method instead of the widely used ∑ 2 p i (1 - p i) could achieve an increase in prediction reliability in most traits. We detected most of the bayesian models had a better performance, especially for BayesN. Compared to GBLUP, BayesN can further improve the predictive reliability with an average of 0.06 for duck carcass traits. Conclusion: This study demonstrates genomic selection for duck carcass traits is promising. The genomic prediction can be further improved by modifying the genomic relationship matrix using our proposed true variance method and several Bayesian models. Permutation study provides a theoretical basis for the fact that low-density arrays can be used to reduce genotype costs in duck genome selection. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

31. Additional file 9 of Genome-wide association and selective sweep analyses reveal genetic loci for FCR of egg production traits in ducks

Author

Liu, Hehe, Wang, Lei, Guo, Zhanbao, Xu, Qian, Fan, Wenlei, Xu, Yaxi, Hu, Jian, Zhang, Yunsheng, Tang, Jing, Xie, Ming, Zhou, Zhengkui, and Hou, Shuisheng

Abstract

Additional file 9: Figure S4. Genome-wide association and selective sweep analyses on the candidate regions associated with FCR. After checking the GWAS association peaks on chromosomes13, 3, and 6, one by one, we observed that they overlapped with the selection target genomic supported by the sliding windows. Each blue and red dot in the image of the selection analysis represents a sliding window, while each purple dot represents an SNP site in the GWAS analysis.

Published
2021
Full Text
View/download PDF

34. Genome-wide association analysis reveal the genetic reasons affect melanin spot accumulation in beak skin of ducks

Author

Liu, Hehe, primary, Wang, Jianmei, additional, Hu, Jian, additional, Wang, Lei, additional, Guo, Zhanbao, additional, Fan, Wenlei, additional, Xu, Yaxi, additional, Liu, Dapeng, additional, Zhang, Yunsheng, additional, Xie, Ming, additional, Tang, Jing, additional, Huang, Wei, additional, Zhang, Qi, additional, Zhou, Zhengkui, additional, and Hou, Shuisheng, additional

Published
2021
Full Text
View/download PDF

35. Genetic variations for egg internal quality of ducks revealed by genome‐wide association study

Author

Liu, Hehe, primary, Zhou, Zhengkui, additional, Hu, Jian, additional, Guo, Zhanbao, additional, Xu, Yaxi, additional, Li, Yanying, additional, Wang, Lei, additional, Fan, Wenlei, additional, Liang, Suyun, additional, Liu, Dapeng, additional, Zhang, Yunsheng, additional, Xie, Ming, additional, Tang, Jing, additional, Huang, Wei, additional, Zhang, Qi, additional, and Hou, Shuisheng, additional

Published
2021
Full Text
View/download PDF

36. A single nucleotide polymorphism variant located in the cis ‐regulatory region of the ABCG2 gene is associated with mallard egg colour

Author

Liu, Hehe, primary, Hu, Jian, additional, Guo, Zhanbao, additional, Fan, Wenlei, additional, Xu, Yaxi, additional, Liang, Suyun, additional, Liu, Dapeng, additional, Zhang, Yunsheng, additional, Xie, Ming, additional, Tang, Jing, additional, Huang, Wei, additional, Zhang, Qi, additional, Xi, Yang, additional, Li, Yanying, additional, Wang, Lei, additional, Ma, Shengchao, additional, Jiang, Yong, additional, Feng, Yulong, additional, Wu, Yongbao, additional, Cao, Junting, additional, Zhou, Zhengkui, additional, and Hou, Shuisheng, additional

Published
2021
Full Text
View/download PDF

37. Vitamin D and diacylglycerol reduce fat deposit and change liver lipid metabolism and caecal microflora in rats fed a high-fat diet

Author

Liu Yanan, Baowei Wang, Ge Wenhua, Zhang Ming'ai, Kong Min, and Fan Wenlei

Subjects
medicine.medical_specialty, food.ingredient, biology, Chemistry, Blood lipids, Lipid metabolism, Metabolism, Soybean oil, Cecum, Fatty acid synthase, food, medicine.anatomical_structure, Endocrinology, Internal medicine, medicine, biology.protein, lipids (amino acids, peptides, and proteins), Carnitine, Diacylglycerol kinase, medicine.drug
Abstract

Vitamin D (VD) is associated with the release of leptin, which promotes fat mobilization in the body. Diacylglycerol can reduce triacylglycerol formation. This study evaluated the effects of administration of VD and duck oil diacylglycerol on blood lipids, liver lipid metabolism and gut microflora in rats fed a high-fat diet. A total of 75 rats were allocated randomly to five treatments: non-obesity (NO), obesity control (OC), diacylglycerol:10 mL per kg body weight (DG10), VD:12.5 μg per kg body weight (VD12.5), and diacylglycerol + VD: 10 mL/d + 12.5 μg per kg body weight (DG10VD12.5). Rats in NO group was fed the diet containing 7% soybean oil, while the other four groups were fed a high-fat (3.3% soybean oil and 30.1% lard). VD and diacylglycerol were orally dosed once per day. The administration of VD+diacylglycerol significantly reduced the body weight gain and fat content. The change of fat deposit was accompanied with the reduction in serum lipids, the increase in the activity of enzymes involved in lipid metabolism in the liver, the suppression of fatty acid synthase and the enhance of carnitine fatty acyl transferase Ⅰ (CPT1) expression. The administration of VD and diacylglycerol also significant modulated the bacterial diversity profile in the cecum towards that in non-obesity rats. Those results suggest that the administration of VD and diacylglycerol could have significant roles in reducing fat deposit and altering gut microflora in rats fed the high-fat diet.

Published
2020

38. Synergistic Effect of Diacylglycerol and Vitamin D in Ameliorating Dextran Sodium Sulfate-Induced Colitis in Rats

Author

Mingai Zhang, Baowei Wang, Ge Wenhua, Xie Yu'e, Xu Huixin, Liu Yanan, Fan Wenlei, and Kong Min

Subjects
0301 basic medicine, Male, Antioxidant, medicine.medical_treatment, Sodium, chemistry.chemical_element, Pharmacology, Biochemistry, Antioxidants, Diglycerides, Rats, Sprague-Dawley, 03 medical and health sciences, chemistry.chemical_compound, Intestinal mucosa, medicine, Vitamin D and neurology, Animals, Colitis, Vitamin D, Diacylglycerol kinase, 030109 nutrition & dietetics, Organic Chemistry, Body Weight, Dextran Sulfate, Drug Synergism, Cell Biology, medicine.disease, Enzymes, Gastrointestinal Microbiome, PPAR gamma, Disease Models, Animal, 030104 developmental biology, Cytokine, Dextran, Ducks, chemistry, Gene Expression Regulation, Intestinal Absorption, Cytokines, Drug Therapy, Combination, Spleen
Abstract

Vitamin D (VD) has the function of antibacteria and protect intestinal mucosa. Diacylglycerol has the property of dissolving VD, anti-bacterial, and antioxidant effects. The purpose of this study was to explore the potentially synergistic effects of diacylglycerol and VD in ameliorating dextran sodium sulfate-induced colitis in rats. A 2 × 3 factorial design was used in this experiment, consisting of two levels of VD (2.5 and 5 μg/day) crossed with three levels of duck oil diacylglycerol (0.5, 1, and 2 mL/day). The experiment lasted for 2 weeks. Compared with the colitis group, the physiological indexes were altered in colitis rats treated with diacylglycerol and VD, the concentrations of the pro-inflammatory indices were significantly reduced, the antioxidant enzyme activities were significantly increased, the diversity of caecal microflora was significantly increased. Besides, the expression of PPARγ was up-regulated while the expression of NF-κBp65 was downregulated. The changes of all those measures were toward those in the Healthy Control, and the mostly appropriate combination was 1 mL/day of diacylglycerol plus 2.5 μg/day VD.

Published
2019

47. Genome-wide association studies demonstrate that TASP1contributes to increased muscle fiber diameter

Author

Liu, Dapeng, Fan, Wenlei, Xu, Yaxi, Yu, Simeng, Liu, Wenjing, Guo, Zhanbao, Huang, Wei, Zhou, Zhengkui, and Hou, Shuisheng

Abstract

Muscle fiber diameter is an economically important trait because it affects meat yield and quality. However, the genetic basis underlying muscle fiber diameter has not been determined. In this study, we collected THREE muscular histological phenotypes in 479 ducks from an F2segregating population generated by mallard × Pekin duck crosses. We performed genome-wide association studies (GWAS) and identified a quantitative trait locus (QTL) significantly associated with muscle fiber diameter on chromosome 3. Then, we discovered the selection signatures using the fixation index among 40 mallards and 30 Pekin ducks in this QTL region. Furthermore, we characterized the recombination event in this QTL region and identified a 6-kb block located on TASP1that was significantly associated with muscle fiber diameter. Finally, five SNPs were screened as potential causative mutations within the 6-kb block. In conclusion, we demonstrated that TASP1contributes to an increase in muscle fiber diameter, which helps to characterize muscle development and contributes to the genetic improvement of meat yield and quality in livestock.

Published
2021
Full Text
View/download PDF

50. Effects of zinc and Bacillus subtilison the reproductive performance, egg quality, nutrient digestion, intestinal morphology and serum antioxidant capacity of geese breeders

Author

Fan, Wenlei, Shi, Jing, Wang, Baowei, Zhang, Mingai, Kong, Min, and Li, Wenli

Abstract

The effects of zinc (Zn) and Bacillus subtilis(B. subtilis) on reproductive performance, egg quality, nutrient digestion, intestine morphology and antioxidant capacity were explored in geese breeders. Geese breeders (n = 120, 46-wk of age) were randomly assigned into 6 groups with 4 replicates of 5 birds each (1 male and 4 female). Breeders were fed diets with two levels of B. subtilis(2.5 × 109and 5 × 109CFU/kg) crossed with three levels of Zn (25, 45 and 65 mg/kg) for duration of 10-weeks. The results showed that the egg laying rate (P< 0.05), fertility rate (P< 0.01), hatchability rate (P< 0.05), yolk color (P< 0.05) and the retentions of crude protein (P< 0.05), ether extract (P< 0.05) and phosphorus of geese breeders were improved by dietary supplementation of 5 × 109CFU/kg B. subtilisand 25 mg or 45 mg/kg Zn. The serum T-SOD (P< 0.05) was increased by 45 mg/kg Zn supplementation. The serum T-AOC (P< 0.05) and retention of Zn (P< 0.05) were increased by 5 × 109CFU/kg B. subtilissupplementation. The birds fed with 5 × 109CFU/kg B. subtilisand 25 mg or 45 mg/kg Zn showed improved villus length (P< 0.01) and villus length/ crypt depth (P< 0.01) in both the jejunum and ileum. In conclusion, the combination of B. subtilisand Zn may have synergistic effects on these parameters, and dietary inclusion of 5 × 109CFU/kg B. subtilisand 45 mg/kg Zn is recommended for improving the reproductive performance of geese breeders.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results