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32 results on '"Fan, Yi-Min"'

11. Isolating Antipathogenic Fungal Coumarins from Coriaria nepalensisand Determining Their Primary Mechanism In Vitro

Author

Jian, Jun-you, Fan, Yi-min, Jin, Jun, He, Xi-yue, Yi, Ping, Yuan, Chun-mao, Gu, Wei, Hu, Zhan-xing, Huang, Lie-jun, and Hao, Xiao-jiang

Abstract

Through bioassay-guided isolation, eight undescribed coumarins (1–8), along with six reported coumarins (9–14), were obtained from Coriaria nepalensis.The new structures were determined by using IR, UV, NMR, HRESIMS, and ECD calculations. The results of the biological activity assays showed that compound 9exhibited broad spectrum antifungal activities against all tested fungi in vitroand a significant inhibitory effect on Phytophthora nicotianaewith an EC50value of 3.00 μg/mL. Notably, compound 9demonstrated greater curative and protective effects against tobacco balack shank than those of osthol in vivo. Thus, 9was structurally modified to obtain new promising antifungal agents, and the novel derivatives (17b, 17j, and 17k) exhibited better effects on Sclerotinia sclerotiorumthan did lead compound 9. Preliminary mechanistic exploration illustrated that 9could enhance cell membrane permeability, destroy the morphology and ultrastructure of cells, and reduce the exopolysaccharide content of P. nicotianaemycelia. Furthermore, the cytotoxicity results revealed that compound 9exhibited relatively low cytotoxicity against HEK293 cell lines with an inhibition rate of 33.54% at 30 μg/mL. This research is promising for the discovery of new fungicides from natural coumarins with satisfactory ecological compatibility.

Published
2024
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18. A New C21-steroidal Cynansteroid O from Roots of Cynanchum auriculatum.

Author

Huang, Lie-jun, Fan, Yi-min, Jin, Jun, Jian, Jun-you, Yuan, Chun-mao, Gu, Wei, and Hao, Xiao-jiang

Subjects
*NATURAL products, *CHEMICAL structure, *NEW product development, *SAPOGENINS, *ASCLEPIADOIDEAE
Abstract

A new C21-steroidal cynansteroid O (1), together with one new natural product 20-hydroxylateqingyangshengenin (2), and three known compounds (3–5) were isolated from the roots of Cynanchum auriculatum. The chemical structures were elucidated by analyses of physio-chemical properties and spectral data and compared with the published data with given references. Compounds 1–3 were isolated for the first time from the roots of C. auriculatum. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Prevalence and molecular characterization of Cryptosporidium spp. in Père David’s deer (Elaphurus davidianus) in Jiangsu, China

Author

Huang, Si-Yang, Fan, Yi-Min, Yang, Yi, Ren, Yi-Jun, Gong, Jing-Zhi, Yao, Na, and Yang, Bin

Subjects
60 kDa glycoprotein (gp60) gene, the small subunit ribosomal RNA (SSU rRNA), Père David’s deer, animal diseases, parasitic diseases, Gene de glicoproteína de 60 kDa (gp60), Cryptosporidium, cervos de Père David, RNA ribossômico da pequena subunidade (SSU rRNA)
Abstract

Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David’s deer. In this study, 137 fecal samples from Père David’s deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David’s deer in this area. Resumo Cryptosporidium é um parasita zoonótico que causa diarreia em uma ampla gama de animais, incluindo veados. Pouco se sabe sobre a prevalência e o genótipo de Cryptosporidium spp. no cervo de Père David. Neste estudo, 137 amostras fecais do cervo de Père David foram coletadas entre julho de 2017 e agosto de 2018, na Reserva Dafeng, e analisadas para Cryptosporidium spp. por nested-PCR baseado no gene do RNA ribossômico da subunidade pequena (SSU rRNA), seguido de análises de sequências para determinar as espécies. O gene da glicoproteína de 60 kDa (gp60) foi utilizado para caracterizar Cryptosporidium spp. Dentre as 137 amostras, 2 (1,46%) foram positivas para Cryptosporidium spp. de acordo com os resultados do sequenciamento gênico de SSU rRNA. Ambas as amostras pertenciam ao genótipo do cervo Cryptosporidium, com duas deleções nucleotídicas e uma substituição nucleotídica. Os dados de prevalência e a caracterização molecular deste estudo fornecem conhecimentos básicos para controlar e prevenir infecções por Cryptosporidium nos cervos de Père David nessa.

Published
2020

31. Limonoids from Munronia henryi and their anti-tobacco mosaic virus activity.

Author

Yan, Ying, Yuan, Chun-Mao, Di, Ying-Tong, Huang, Tao, Fan, Yi-Min, Ma, Yuan, Zhang, Jian-Xin, and Hao, Xiao-Jiang

Subjects
*ALTERNATIVE medicine, *ANTIVIRAL agents, *DOSE-effect relationship in pharmacology, *MEDICINAL plants, *MICROBIAL sensitivity tests, *PLANT diseases, *SPECTRUM analysis, *WESTERN immunoblotting, *PLANT extracts, *DESCRIPTIVE statistics, *IN vitro studies, *PHARMACODYNAMICS
Abstract

Five new compounds ( 1 – 5 ), including three limonoids, one diterpenoid, and one phytosterol, along with four known limonoids ( 6 – 9 ), were isolated from the ethanolic extracts of whole plants of Munronia henryi . Their structures were elucidated by extensive spectroscopic analysis. In addition, the anti-tobacco mosaic virus (TMV) activities of all the isolated compounds were evaluated by the conventional half-leaf and leaf-disk methods along with Western blot analysis. Most of the tested compounds showed strong antiviral activities, with IC 50 values in the range of 14.8–34.0 μg/mL, compared with ningnanmycin as a positive control (IC 50 = 44.6 μg/mL), and their preliminary structure-activity relationships were also discussed. [ABSTRACT FROM AUTHOR]

Published
2015
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32. PruΔcdpk2 Protects Pigs against Acute Toxoplasmosis Depending on T-Lymphocyte Subsets and Natural Killer Cell Responses.

Author

Fan YM, Shi WQ, Jin QW, Pan M, Hou ZF, Fu L, Tao JP, and Huang SY

Abstract

Toxoplasma gondii is a widespread protozoan parasite approximately infecting one-third of the world population and can cause serious public health problems. In this study, we investigated the protective effect of the attenuated vaccine Pru:Δcdpk2 against acute toxoplasmosis and explored the underlying immune mechanisms of the protection in pigs. The systemic T-cell and natural killer (NK) cell responses were analyzed, including kinetics, phenotype, and multifunctionality (interferon [IFN]-γ, tumor necrosis factor [TNF]-α), and the IFN-γ levels were analyzed in PBMCs. Our results showed that T. gondii -specific antibodies were induced by Pru:Δcdpk2. After challenging with RH, the antibodies were able to respond quickly in the immunized group, and the expression level was significantly higher than that in the unimmunized group. The expression level of IFN-γ significantly increased after vaccination, and the CD3+ γδ-, NK, and CD3+ γδ+ cell subsets also significantly increased. At the same time, functional analysis indicated that these cells were polarized toward a Th1 phenotype, showing the ability to secrete IFN-γ and TNF-α. The CD4+CD8α-T cell population exhibited a higher frequency of IFN-γ+ producing cells compared with the CD4-CD8α+ and CD4+CD8α+ cell populations during the early days of vaccination. Our results indicated that the attenuated vaccine could induce the expression of NK, γδ, and CD3αβ cells in pigs, and IFN-γ and TNF-α secreted by these cells are important for resistance to T. gondii infection.

Published
2024
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