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238 results on '"Fang, Liang-Xing"'

4. Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli

Author

Sun, Jian, Chen, Chong, Cui, Chao-Yue, Zhang, Yan, Liu, Xiao, Cui, Ze-Hua, Ma, Xiao-Yu, Feng, Youjun, Fang, Liang-Xing, Lian, Xin-Lei, Zhang, Rong-Min, Tang, You-Zhi, Zhang, Kou-Xing, Liu, Han-Mian, Zhuang, Zhi-Hui, Zhou, Shi-Dan, Lv, Jing-Nan, Du, Hong, Huang, Bin, Yu, Fang-You, Mathema, Barun, Kreiswirth, Barry N., Liao, Xiao-Ping, Chen, Liang, and Liu, Ya-Hong

Published
2019
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6. Phentolamine Significantly Enhances Macrolide Antibiotic Antibacterial Activity against MDR Gram-Negative Bacteria

Author

Cui, Ze-Hua, primary, He, Hui-Ling, additional, Zheng, Zi-Jian, additional, Yuan, Zhao-Qi, additional, Chen, Ying, additional, Huang, Xin-Yi, additional, Ren, Hao, additional, Zhou, Yu-Feng, additional, Zhao, Dong-Hao, additional, Fang, Liang-Xing, additional, Yu, Yang, additional, Liu, Ya-Hong, additional, Liao, Xiao-Ping, additional, and Sun, Jian, additional

Published
2023
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7. CRISPR-AMRtracker: a novel toolkit to monitor the antimicrobial resistance gene transfer in fecal microbiota

Author

Li, Gong, primary, Long, Tengfei, additional, Zhou, Shiying, additional, Xia, Lijuan, additional, Wan, Lei, additional, Diao, Xiaoyuan, additional, Gao, Ang, additional, He, Yuzhang, additional, Sun, Ruan-Yang, additional, Ren, Hao, additional, Fang, Liang-Xing, additional, Liao, Xiao-Ping, additional, Liu, Ya-Hong, additional, Chen, Liang, additional, and Sun, Jian, additional

Published
2023
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8. ARSCP: An antimicrobial residue surveillance cloud platform for animal-derived foods

Author

Yu, Jun-Jun, primary, Hu, Ya-Lin, additional, Liu, Cheng-Zheng, additional, Wu, Shuai-Bin, additional, Zheng, Zi-Jian, additional, Cui, Ze-Hua, additional, Chen, Li, additional, Wei, Tian, additional, Sun, Shi-Kai, additional, Ning, Jun, additional, Wen, Xiang, additional, Diao, Qiu-Yue, additional, Yu, Yang, additional, Fang, Liang-Xing, additional, Zhou, Yu-Feng, additional, Liu, Ya-Hong, additional, Liao, Xiao-Ping, additional, Li, Xi-Ming, additional, and Sun, Jian, additional

Published
2023
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9. Environmental remodeling of human gut microbiota and antibiotic resistome in livestock farms

Author

Sun, Jian, Liao, Xiao-Ping, D’Souza, Alaric W., Boolchandani, Manish, Li, Sheng-Hui, Cheng, Ke, Luis Martínez, José, Li, Liang, Feng, You-Jun, Fang, Liang-Xing, Huang, Ting, Xia, Jing, Yu, Yang, Zhou, Yu-Feng, Sun, Yong-Xue, Deng, Xian-Bo, Zeng, Zhen-Ling, Jiang, Hong-Xia, Fang, Bing-Hu, Tang, You-Zhi, Lian, Xin-Lei, Zhang, Rong-Min, Fang, Zhi-Wei, Yan, Qiu-Long, Dantas, Gautam, and Liu, Ya-Hong

Published
2020
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13. Molecular Epidemiology of Plasmid-Mediated Types 1 and 3 Fimbriae Associated with Biofilm Formation in Multidrug Resistant Escherichia coli from Diseased Food Animals in Guangdong, China

Author

Guo, Wen-Ying, primary, Zhang, Hui, additional, Cheng, Ming, additional, Huang, Min-Rui, additional, Li, Qian, additional, Jiang, Yu-Wei, additional, Zhang, Ji-Xing, additional, Sun, Ruan-Yang, additional, Wang, Min-Ge, additional, Liao, Xiao-Ping, additional, Liu, Ya-Hong, additional, Sun, Jian, additional, and Fang, Liang-Xing, additional

Published
2022
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19. Comprehensive analysis of plasmid-mediated tet(X4)-positive Escherichia coli isolates from clinical settings revealed a high correlation with animals and environments-derived strains

Author

Cui, Chao-Yue, primary, Li, Xiao-Jie, additional, Chen, Chong, additional, Wu, Xiao-Ting, additional, He, Qian, additional, Jia, Qiu-Lin, additional, Zhang, Xiao-Jing, additional, Lin, Zhuo-Yu, additional, Li, Cang, additional, Fang, Liang-Xing, additional, Liao, Xiao-Ping, additional, Liu, Ya-Hong, additional, Hu, Bo, additional, and Sun, Jian, additional

Published
2022
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20. Supplementary materials submitted to figshare.docx

Author

Zhang, Jin-Fei, Fang, Liang-Xing, Chang, Man-Xia, Cheng, Ming, Zhang, Hui, Long, Teng-Fei, Li, Qian, Lian, Xin-lei, Sun, Jian, Liao, Xiao-Ping, and Liu, Ya-Hong

Abstract

A trade-off for maintenance of multidrug-resistance IncHI2 plasmids in Salmonella Typhimurium through adaptive evolution

Published
2022
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21. MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria

Author

Zheng, Zi-Jian, primary, Cui, Ze-Hua, additional, Diao, Qiu-Yue, additional, Ye, Xin-Qing, additional, Zhong, Zi-Xing, additional, Tang, Tian, additional, Wu, Shuai-Bin, additional, He, Hui-Ling, additional, Lian, Xin-Lei, additional, Fang, Liang-Xing, additional, Wang, Xi-Ran, additional, Liang, Li-Jie, additional, Liu, Ya-Hong, additional, Liao, Xiao-Ping, additional, and Sun, Jian, additional

Published
2021
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29. Duck wastes as a potential reservoir of novel antibiotic resistance genes

Author

Wang, Xi-Ran, primary, Lian, Xin-Lei, additional, Su, Tian-Tian, additional, Long, Teng-Fei, additional, Li, Meng-Yuan, additional, Feng, Xiao-Yin, additional, Sun, Ruan-Yang, additional, Cui, Ze-Hua, additional, Tang, Tian, additional, Xia, Jing, additional, Huang, Ting, additional, Liu, Ya-Hong, additional, Liao, Xiao-Ping, additional, Fang, Liang-Xing, additional, and Sun, Jian, additional

Published
2021
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31. MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria

Author

Zheng, Zi-Jian, Cui, Ze-Hua, Diao, Qiu-Yue, Ye, Xin-Qing, Zhong, Zi-Xing, Tang, Tian, Wu, Shuai-Bin, He, Hui-Ling, Lian, Xin-Lei, Fang, Liang-Xing, Wang, Xi-Ran, Liang, Li-Jie, Liu, Ya-Hong, Liao, Xiao-Ping, and Sun, Jian

Abstract

ABSTRACTThe extensive use of tetracycline antibiotics has led to the widespread presence of tetracycline-resistance genes in Gram-negative bacteria and this poses serious threats to human and animal health. In our previous study, we reported a method for rapid detection of Tet(X)-producers using MALDI-TOF MS. However, there have been multiple machineries involved in tetracycline resistance including efflux pump, and ribosomal protection protein. Our previous demonstrated the limitation in probing the non-Tet(X)-producing tetracycline-resistant strains. In this regard, we further developed a MALDI-TOF MS method to detect and differentiate Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant strains. Test strains were incubated with tigecycline and oxytetracycline in separate tubes for 3 h and then analyzed spectral peaks of tigecycline, oxytetracycline, and their metabolite. Strains were distinguished using MS ratio for [metabolite/(metabolite+ tigecycline or oxytetracycline)]. Four control strains and 319 test strains were analyzed and the sensitivity was 98.90% and specificity was 98.34%. This was consistent with the results obtained from LC-MS/MS analysis. Interestingly, we also found that the reactive oxygen species (ROS) produced by tetracycline-susceptible strains were able to promote the degradation of oxytetracycline. Overall, the MALDITet(X)-plustest represents a rapid and reliable method to detect Tet(X)-producers, non-Tet(X)-producing tetracycline-resistant strains, and tetracycline-susceptible strains.

Published
2022
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32. Epidemiology of bla CTX-M-Positive Salmonella Typhimurium From Diarrhoeal Outpatients in Guangdong, China, 2010–2017.

Author

Jiang, Qi, Ke, Bi-xia, Wu, De-shu, Wang, Dong, Fang, Liang-xing, Sun, Ruan-yang, Wang, Min-ge, Lei, Jing-er, Shao, Zheng, and Liao, Xiao-ping

Subjects
SALMONELLA typhimurium, OUTPATIENTS, EPIDEMIOLOGY, NUCLEOTIDE sequencing, PLASMIDS
Abstract

Salmonella enterica can lead to intestinal diarrhea, and the emergence and spread of cephalosporin-resistant Salmonella have brought great challenges to clinical treatment. Therefore, this study investigated the prevalence and transmission of bla CTX-M genes among S. Typhimurium from diarrhoeal outpatients in Guangdong, China, from 2010 to 2017. A total of 221 bla CTX-M-positive isolates were recovered from 1,263 S. Typhimurium isolates from the facal samples of diarrhoea patients in 45 general hospitals from 11 cities. The most popular CTX-M gene was bla CTX-M-55 (39.6%, 72/182) in the CTX-M-1 group, followed by bla CTX-M-14 (22.5%, 41/182) and bla CTX-M-65 (19.2%, 35/182) in the CTX-M-9 group. The isolates that carried bla CTX-M-9G had significantly higher resistance rates to multiple antibacterials compared with bla CTX-M-1G (p < 0.01). Meanwhile, PFGE analysis not only showed the clonal transmission of bla CTX-M-55/14/65-positve isolates of diarrhoeal outpatients' origins from different hospitals in Guangdong province, but also the characteristic of bla CTX-M-55/14/65-positve isolates' bacterial persistence. Multilocus sequence typing (MLST) analysis indicated that these S. Typhimurium isolates possessed ST34 and ST19. Furthermore, genomic Beast phylogenomic analysis provided the evidence of a close relationship of bla CTX-M-positive S. Typhimurium isolates between the outpatients and pork. Most bla CTX-M-55/14/65 genes were transmitted by non-typeable or IncI1/IncFII/IncHI2 plasmids with the size of ranging from ~80 to ~280 kb. Moreover, whole-genome sequencing (WGS) analysis further revealed that bla CTX-M-55/14/65 coexisted with other 25 types of ARGs, of which 11 ARGs were highly prevalent with the detection rates >50%, and it first reported the emergence of bla TEM-141 in S. Typhimurium. This study underscores the importance of surveillance for bla CTX-M-positive microbes in diarrhea patients. [ABSTRACT FROM AUTHOR]

Published
2022
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33. Comparison of the prevalence and molecular characteristics of fosA3 and fosA7 among Salmonella isolates from food animals in China.

Author

Wang, Dong, Fang, Liang-Xing, Jiang, Yu-Wei, Wu, De-Shu, Jiang, Qi, Sun, Ruan-Yang, Wang, Min-Ge, Sun, Jian, Liu, Ya-Hong, and Liao, Xiao-Ping

Subjects
*FOOD of animal origin, *SALMONELLA, *FOOD animals, *FOS oncogenes, *SALMONELLA typhimurium
Abstract

Objectives: To investigate the prevalence and molecular characteristics of fosA3 and fosA7 among Salmonella isolates.Methods: Five hundred and fifty-one Salmonella isolates collected from food animals in China during 2016-19 were screened for fos genes. The drug resistance, serovars, clonal relationships and genetic environments of fosA were compared between fosA7- and fosA3-positive Salmonella.Results: A relatively high prevalence of fosA7 (9.26%) and fosA3 (6.53%) was identified. fosA3 was associated with high-level fosfomycin resistance (≥512 mg/L), while fosA7 conferred relatively low-level resistance that was independent of the presence of glucose-6-phosphate. Additionally, fosA7 could facilitate Salmonella survival under oxidative stress. Both fosA3 and fosA7 were found in diverse serovars and STs, but segregated into distinct groups. The fosA3-positive Salmonella Typhimurium/Salmonella Indiana strains showed close genetic relationships, while fosA7-positive Salmonella Meleagridis/Salmonella Agona/Salmonella Derby showed a relatively high degree of whole-genome sequence heterogeneity. fosA3 was located on conjugative IncHI2 plasmids or chromosomes, while fosA7 was strictly chromosomal. Furthermore, two strains carried large chromosomal fosA7 regions within genomic islands. The fosA3 and fosA7 contigs from our isolates and the NCBI could be segregated into four primary and distinct genomic backbones. IS26 and the antibiotic resistance genes (ARGs) blaCTX-M, blaTEM-1B and rmtB were frequently adjacent to fosA3, while fosA7-carrying contigs generally lacked mobile elements and ARGs.Conclusions: fosA3 and fosA7 were the primary factors contributing to reduced fosfomycin susceptibility, to different degrees, in these Salmonella isolates. The distinct distributions and molecular characteristics of fosA7 and fosA3 indicated that their origin and evolution in Salmonella were most likely distinct. [ABSTRACT FROM AUTHOR]

Published
2022
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34. Occurrence and Transmission of bla NDM -Carrying Enterobacteriaceae from Geese and the Surrounding Environment on a Commercial Goose Farm

Author

Cen, Dao-Ji, primary, Sun, Ruan-Yang, additional, Mai, Jia-Lin, additional, Jiang, Yu-Wei, additional, Wang, Dong, additional, Guo, Wen-Ying, additional, Jiang, Qi, additional, Zhang, Hui, additional, Zhang, Jin-Fei, additional, Zhang, Rong-Min, additional, Sun, Jian, additional, Liao, Xiao-Ping, additional, Liu, Ya-Hong, additional, and Fang, Liang-Xing, additional

Published
2021
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37. Emergence of fosA3 and blaCTX–M–14 in Multidrug-Resistant Citrobacter freundii Isolates From Flowers and the Retail Environment in China

Author

Cheng, Ke, primary, Fang, Liang-Xing, additional, Ge, Qian-Wen, additional, Wang, Dong, additional, He, Bing, additional, Lu, Jia-Qi, additional, Zhong, Zi-Xing, additional, Wang, Xi-Ran, additional, Yu, Yang, additional, Lian, Xin-Lei, additional, Liao, Xiao-Ping, additional, Sun, Jian, additional, and Liu, Ya-Hong, additional

Published
2021
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39. Transmission and molecular characteristics of blaNDM-producing Escherichia coli between companion animals and their healthcare providers in Guangzhou, China.

Author

Wang, Min-Ge, Fang, Chang, Liu, Kai-Di, Wang, Lin-Lin, Sun, Ruan-Yang, Zhang, Rong-Min, Fang, Liang-Xing, Sun, Jian, Liu, Ya-Hong, and Liao, Xiao-Ping

Subjects
MEDICAL personnel, ESCHERICHIA coli, KLEBSIELLA pneumoniae, PLASMIDS, KLEBSIELLA, RESEARCH, ANIMAL experimentation, RESEARCH methodology, PETS, EVALUATION research, HYDROLASES, COMPARATIVE studies, GENES, RESEARCH funding, ANTIBIOTICS, MICROBIAL sensitivity tests
Abstract

Objectives: To determine the transmission and molecular characteristics of blaNDM-producing Escherichia coli between companion animals and their healthcare providers at veterinary clinics in Guangzhou, China.Methods: A total of 359 samples from companion animals and their healthcare providers were collected at 14 veterinary clinics in Guangzhou, China. Genomic characteristics and clonal relationships for blaNDM-positive E. coli and complete plasmid sequences were characterized based on WGS data from combined Illumina and MinION platform reads.Results: Forty-five blaNDM-positive bacteria were recovered from companion animals (n = 43) and their healthcare providers (n = 2) at 10 veterinary clinics. Overall, E. coli (73.3%, 33/45) and Klebsiella pneumoniae (13.3%, 6/45) were the most prevalent species among the seven species of blaNDM-positive bacteria. Four blaNDM variants (blaNDM-1, blaNDM-4, blaNDM-5 and blaNDM-7) were identified in 45 blaNDM-positive bacteria and blaNDM-5 was the most prevalent (77.8%, 35/45). WGS indicated that the most prevalent STs were ST405 (8/33), ST453 (6/33), ST457 (6/33) and ST410 (5/33) among the 33 blaNDM-positive E. coli isolates. Phylogenomics and PFGE analysis revealed that clonal spread of blaNDM-positive ST453 E. coli isolates between companion animals and their healthcare providers was evident. In addition, two novel IncFIB plasmids carrying blaNDM-4 (pF765_FIB and pG908_FIB) were found in this study and indicated that IS26 may promote the horizontal transmission of blaNDM between different plasmid types.Conclusions: In this study we conducted a large-scale investigation on the prevalence of blaNDM-positive E. coli isolates from companion animals and their healthcare providers and revealed the clonal spread of blaNDM-positive E. coli isolates between these two groups. [ABSTRACT FROM AUTHOR]

Published
2022
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43. Rapid Detection of High-Level Tigecycline Resistance in Tet(X)-Producing Escherichia coli and Acinetobacter spp. Based on MALDI-TOF MS

Author

Cui, Ze-Hua, primary, Zheng, Zi-Jian, additional, Tang, Tian, additional, Zhong, Zi-Xing, additional, Cui, Chao-Yue, additional, Lian, Xin-Lei, additional, Fang, Liang-Xing, additional, He, Qian, additional, Wang, Xi-Ran, additional, Chen, Chong, additional, He, Bing, additional, Wang, Min-Ge, additional, Liu, Ya-Hong, additional, Liao, Xiao-Ping, additional, and Sun, Jian, additional

Published
2020
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50. Global clonal spread of mcr-3-carrying MDR ST34 Salmonella enterica serotype Typhimurium and monophasic 1,4,[5],12:i:− variants from clinical isolates

Author

Sun, Ruan-Yang, primary, Ke, Bi-Xia, primary, Fang, Liang-Xing, primary, Guo, Wen-Ying, primary, Li, Xing-Ping, primary, Yu, Yang, primary, Zheng, Si-Lin, primary, Jiang, Yu-Wei, primary, He, Dong-Mei, primary, Sun, Jian, primary, Ke, Chang-Wen, primary, Liu, Ya-Hong, primary, and Liao, Xiao-Ping, primary

Published
2020
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