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156 results on '"Fang, Shiuh‐Bin"'

11. An Antibody of the Secreted Isoform of Disintegrin and Metalloprotease 9 (sADAM9) Inhibits Epithelial–Mesenchymal Transition and Migration of Prostate Cancer Cell Lines.

Author

Jotatsu, Yura, Sung, Shain-Ying, Wu, Ming-Heng, Takeda, Shunya, Hirata, Yuto, Maeda, Koki, Fang, Shiuh-Bin, Chen, Kuan-Chou, and Shigemura, Katsumi

Subjects
CANCER cell migration, EPITHELIAL-mesenchymal transition, CASTRATION-resistant prostate cancer, CELL lines, ANDROGEN deprivation therapy, SLEEP deprivation, CADHERINS, ANDROGEN receptors
Abstract

Prostate cancer (PC) is the most common cancer diagnosed in men worldwide. Currently, castration-resistant prostate cancer (CRPC), which is resistant to androgen deprivation therapy, has a poor prognosis and is a therapeutic problem. We investigated the antitumor effects on PC of an antibody neutralizing secreted disintegrin and metalloproteinase domain-containing protein 9 (sADAM9), which is a blood-soluble form. We performed proliferation assays, wound healing assays, invasion assays, Western blot (WB), and an in vivo study in which a sADAM9 neutralizing antibody was administered intratumorally to PC-bearing mice. In invasion assays, the sADAM9 neutralizing antibody significantly inhibited invasion in all cell lines (TRAMP-C2: p = 0.00776, LNCaP: p = 0.000914, PC-3: p = 0.0327, and DU145: p = 0.0254). We examined epithelial–mesenchymal transition (EMT) markers, one of the metastatic mechanisms, in WB and showed downregulation of Slug in TRAMP-C2, LNCaP, and DU145 and upregulation of E-cadherin in TRAMP-C2 and PC-3 by sADAM9 neutralization. In mouse experiments, the sADAM9 neutralizing antibody significantly suppressed tumor growth compared to controls (1.68-fold in TRAMP-C2, 1.89-fold in LNCaP, and 2.67-fold in PC-3). These results suggested that the sADAM9 neutralizing antibody inhibits invasion, migration, and tumor growth in PC. Previous studies examined the anti-tumor effect of knockdown of total ADAM9 or sADAM9, but this study used the new technology of neutralizing antibodies for sADAM9. This may be novel because there was no animal study using a neutralizing antibody for sADAM9 to see the relationship between ADAM9 expression and prostate cancer. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Response to: Grummer‐Strawn LM, Lutter CK, Siegfried N, et al. Response to: World Health Organization (WHO) guideline on the complementary feeding of infants and young children aged 6–23 months 2023: A multisociety response. JPGN 2024

Author

Fewtrell, Mary, Baumann, Ulrich, Bronsky, Jiri, Haiden, Nadja, Hill, Susan, Kivelä, Laura, Koenig, Barbara, Kӧglmeier, Jutta, Luque, Veronica, Moltu, Sissel J., Norsa, Lorenzo, Savino, Francesco, Verduci, Elvira, Allied, Julie Lanigan, Banci, Elena, Mustapha, Moriam, Asseiceira, Ines, Malmberg, Elin, Stróżyk, Agata, Schoeler, Natasha Schoeler, Niseteo, Tena, Koletzko, Berthold, European, Liviana da Dalt, Young, Nora Karara Chair, De Guchtenaere, Ann, Treasurer, Łukasz Dembiński, Grossman, Zachi, Hadjipanayis, Adamos, Stordal, Ketil, Boode, Willem P., Pipaon, Miguel Saenz, Allied, Rosan Meyer, Arasi, Stefania, Marina, Orsi, Vázquez‐Frias, Rodrigo, Ladino‐Meléndez, Liliana, Ochoa‐Ortiz, Erika, Spolidoro, José, D'Agostino, Daniel, El‐Guindi, Mohamed, Zekri, Maha Abou, Mouane, Nezha, El Awad, Mamoun, Rawashdeh, Mohammad, Sabir, Omayma, Koleilat, Aziz, Hidra, Aida, Fang, Shiuh‐Bin, Arai, Katsuhiro, Treepongkaruna, Suporn, Kim, Kyung Mo, Goday, Praveen S., Duro, Debora, Lightdale, Jenifer R., Martin, Bryan, Morais‐Almeida, Mario, Wong, Gary W. K., Ebisawa, Motohiro, Counselor, Ignacio Ansotegui, and Fiocchi, Alessandro

Published
2024
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14. World Health Organization (WHO) guideline on the complementary feeding of infants and young children aged 6−23 months 2023: A multisociety response

Author

Fewtrell, Mary, Baumann, Ulrich, Bronsky, Jiri, Haiden, Nadja, Hill, Susan, Kivelä, Laura, Koenig, Barbara, Kӧglmeier, Jutta, Luque, Veronica, Moltu, Sissel J., Norsa, Lorenzo, Savino, Francesco, Verduci, Elvira, Lanigan, Julie, Banci, Elena, Mustapha, Moriam, Asseiceira, Ines, Segerstad, Elin Malmberg Hård af, Stróżyk, Agata, Schoeler, Natasha Schoeler, Niseteo, Tena, Koletzko, Berthold, da Dalt, Liviana, Karara, Nora, De Guchtenaere, Ann, Dembiński, Łukasz, Grossman, Zachi, Hadjipanayis, Adamos, Stordal, Ketil, Boode, Willem P., Pipaon, Miguel Saenz, Meyer, Rosan, Arasi, Stefania, Marina, Orsi, Vázquez‐Frias, Rodrigo, Ladino‐Meléndez, Liliana, Ochoa‐Ortiz, Erika, Spolidoro, José, D'Agostino, Daniel, El‐Guindi, Mohamed, Zekri, Maha Abou, Mouane, Nezha, Awad, Mamoun El, Rawashdeh, Mohammad, Sabir, Omayma, Koleilat, Aziz, Hidra, Aida, Fang, Shiuh‐Bin, Arai, Katsuhiro, Treepongkaruna, Suporn, Kim, Kyung Mo, Goday, Praveen S., Duro, Debora, Lightdale, Jenifer R., Martin, Bryan, Morais‐Almeida, Mario, Wong, Gary W. K., Ebisawa, Motohiro, Ansotegui, Ignacio, Fiocchi, Alessandro, Wong, Gary W. K., and Ebisawa, Motohiro

Abstract

The recent World Health Organization (WHO) guideline aims to provide evidence‐based recommendations on complementary feeding (CF) of healthy term infants and young children 6−23 months living in low‐, middle‐, and high‐income countries, including both breastfed and non‐breastfed children. Like WHO, our organizations aim to promote optimal infant and young child nutrition and health, with a focus on promoting breastfeeding as well as appropriate and timely CF. In this paper, we share our concerns about aspects of the guideline, some of which may have the potential to cause unintended harm in infants and young children and suggest alternative or modified proposals.

Published
2024
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21. Genetic characteristics of azithromycin-resistant Neisseria gonorrhoeae collected in Hyogo, Japan during 2015–2019

Author

Miura, Makiko, primary, Shigemura, Katsumi, additional, Osawa, Kayo, additional, Nakanishi, Noriko, additional, Nomoto, Ryohei, additional, Onishi, Reo, additional, Yoshida, Hiroyuki, additional, Sawamura, Toru, additional, Fang, Shiuh-Bin, additional, Chiang, Yi-Te, additional, Sung, Shian-ying, additional, Chen, Kuan-cho, additional, Miyara, Takayuki, additional, and Fujisawa, Masato, additional

Published
2022
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22. Case Report: Proteinase 3 Antineutrophil Cytoplasmic Antibody-Associated Ulcerative Colitis Presenting as Recurrent Intestinal Pseudo-Obstruction in a Teenage Patient With in situ Proteinase 3 Immunohistochemical Staining

Author

Yang, Ching-Wen, primary, Kao, Yu-Chien, additional, Lin, Pei-Chun, additional, Chien, Hsi-Yuan, additional, Lin, Sheng-Chieh, additional, Lee, Yu-Hsien, additional, Huang, Yen-Lin, additional, and Fang, Shiuh-Bin, additional

Published
2022
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24. Molecular characteristics and genetic analysis of the genes related to carbapenemase, efflux pump, and outer membrane proteins in carbapenem-resistant Klebsiella pneumoniae.

Author

Onishi, Reo, Shigemura, Katsumi, Osawa, Kayo, Yang, Young-Min, Maeda, Koki, Tanimoto, Hiroshi, Kado, Mitsuki, Fang, Shiuh-Bin, and Fujisawa, Masato

Subjects
CARBAPENEM-resistant bacteria, KLEBSIELLA pneumoniae, CARBAPENEMASE, MEMBRANE proteins, CARBAPENEMS, ERTAPENEM
Abstract

Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a life-threatening pathogen that has not been fully investigated on a molecular basis. Therefore, the molecular mechanisms of carbapenem resistance in CRKP collected from medical institutions in Hyogo Prefecture has been analyzed. Antimicrobial susceptibilities and the presence of carbapenemase along with epidemiological analyzes using multilocus sequence typing (MLST) have been investigated. The relative expression of efflux pump genes and mutations of ompK35 and ompK36 , encoding the outer membrane porin, were also assessed for their relationship with carbapenem resistance. Most of the collected 22 CRKP isolates were non-susceptible to imipenem (68.2%), meropenem (90.9%), and ertapenem (81.8%), but all 22 strains were susceptible to colistin. Twelve strains (54.5%) were detected for carbapenemase genes such as bla IMP-6. Sequence type 37 was detected by MLST in 10 strains (45.5%). Non-carbapenemase-producing strains had high resistance rates for three carbapenems, and the main cause of resistance was ompK35 mutation. In conclusion, the main cause of resistance was imipenemase metallo-β-lactamase (IMP-6) production in carbapenemase-producing strains, and ompK35 mutation in non-carbapenemase-producing strains. Susceptibility to carbapenem did not differ in CRKP regardless of carbapenemase production, except for imipenem susceptibility. This result contributes to a more insightful understanding of the mechanisms of CRKP in Japan. [ABSTRACT FROM AUTHOR]

Published
2023
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25. Impact on quinolone resistance of plasmid-mediated quinolone resistance gene and mutations in quinolone resistance-determining regions in extended spectrum beta lactamase-producing Klebsiella pneumoniae isolated from urinary tract infection patients

Author

Onishi, Reo, primary, Shigemura, Katsumi, additional, Osawa, Kayo, additional, Yang, Young-Min, additional, Maeda, Koki, additional, Tanimoto, Hiroshi, additional, Kado, Mitsuki, additional, Fang, Shiuh-Bin, additional, Sung, Shian-Ying, additional, Miyara, Takayuki, additional, and Fujisawa, Masato, additional

Published
2022
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27. Genotypic Diversity of Ciprofloxacin Nonsusceptibility and Its Relationship with Minimum Inhibitory Concentrations in Nontyphoidal Salmonella Clinical Isolates in Taiwan

Author

Fang, Shiuh-Bin, primary, Lauderdale, Tsai-Ling Yang, additional, Huang, Chih-Hung, additional, Chang, Pei-Ru, additional, Wang, Yuan-Hung, additional, Shigemura, Katsumi, additional, Lin, Ying-Hsiu, additional, Chang, Wei-Chiao, additional, Wang, Ke-Chuan, additional, Huang, Tzu-Wen, additional, and Chang, Yu-Chu, additional

Published
2021
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31. Comparative genetic analysis of the antimicrobial susceptibilities and virulence of hypermucoviscous and non-hypermucoviscous ESBL-producing Klebsiella pneumoniaein Japan

Author

Tanimoto, Hiroshi, Shigemura, Katsumi, Osawa, Kayo, Kado, Mitsuki, Onishi, Reo, Fang, Shiuh-Bin, Sung, Shian-Ying, Miyara, Takayuki, and Fujisawa, Masato

Abstract

Hypermucoviscous (HMV) Klebsiella pneumoniaeproduces large amounts of capsular polysaccharides, leading to high mortality. Since extended spectrum beta-lactamase (ESBL)-producing HMV K. pneumoniaestrains have increased in Japan, we investigated and compared the antimicrobial susceptibilities and genetic characteristics of HMV and non-HMV ESBL-producing K. pneumoniae.

Published
2023
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34. Genotypic diversity of ciprofloxacin nonsusceptibility and its relationship with minimum inhibitory concentrations in nontyphoidal salmonella clinical isolates in taiwan

Author

Fang, Shiuh Bin, Lauderdale, Tsai Ling Yang, Huang, Chih Hung, Chang, Pei Ru, Wang, Yuan Hung, Shigemura, Katsumi, Lin, Ying Hsiu, Chang, Wei Chiao, Wang, Ke Chuan, Huang, Tzu Wen, Chang, Yu Chu, Fang, Shiuh Bin, Lauderdale, Tsai Ling Yang, Huang, Chih Hung, Chang, Pei Ru, Wang, Yuan Hung, Shigemura, Katsumi, Lin, Ying Hsiu, Chang, Wei Chiao, Wang, Ke Chuan, Huang, Tzu Wen, and Chang, Yu Chu

Abstract

This study analyzed the genetic diversity of ciprofloxacin (CIP) nonsusceptibility and the relationship between two major mechanisms and minimum inhibitory concentrations (MICs) of CIP in nontyphoidal Salmonella (NTS). Chromosomal mutations in quinolone resistance-determining regions (QRDRs) and plasmid-mediated quinolone resistance (PMQR) genes were searched from ResFinder, ARG-ANNOT, and PubMed for designing the sequencing regions in gyrA, gyrB, parC, and parE, and the 13 polymerase chain reactions for PMQR genes. We found that QRDR mutations were detected in gyrA (82.1%), parC (59.0%), and parE (20.5%) but not in gyrB among the 39 isolates. Five of the 13 PMQR genes were identified, including oqxA (28.2%), oqxB (28.2%), qnrS (18.0%), aac(6′ )-Ib-cr (10.3%), and qnrB (5.1%), which correlated with the MICs of CIP within 0.25–2 µg/mL, and it was found that oxqAB contributed more than qnr genes to increase the MICs. All the isolates contained either QRDR mutations (53.8%), PMQR genes (15.4%), or both (30.8%). QRDR mutations (84.6%) were more commonly detected than PMQR genes (46.2%). QRDR mutation numbers were significantly associated with MICs (p < 0.001). Double mutations in gyrA and parC determined high CIP resistance (MICs ≥ 4 µg/mL). PMQR genes contributed to intermediate to low CIP resistance (MICs 0.25–2 µg/mL), thus providing insights into mechanisms underlying CIP resistance.

Published
2021

44. Pseudomonas aeruginosasepsis presenting as oral ecthyma gangrenosum in identical twins with Bruton tyrosine kinase gene mutation: Two case reports and review of the literature

Author

Lin, Sheng-Chieh, Chiang, Bor-Luen, Lee, Yuarn-Jang, Chang, Ya-Ting, and Fang, Shiuh-Bin

Abstract

X-linked agammaglobulinemia (XLA) is a primary immunodeficiency disease. We reported two 7-month-old identical male twins with Pseudomonas aeruginosasepsis who initially manifested as oral ecthyma gangrenosum and were finally diagnosed to have XLA. In both cases, we confirmed the c.862C > T BTKmissense mutation in exon 10 at the SH2 domain.

Published
2020
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47. Comparison of global transcriptomes for nontyphoidal Salmonellaclinical isolates from pediatric patients with and without bacteremia after their interaction with human intestinal epithelial cells in vitro

Author

Batsaikhan, Buyandelger, Lin, Pei-Chun, Shigemura, Katsumi, Wu, Yu-Wei, Onishi, Reo, Chang, Pei-Ru, Cheng, Hung-Yen, and Fang, Shiuh-Bin

Abstract

Nontyphoidal Salmonella(NTS) outbreaks of invasive diseases are increasing. Whether the genetic diversity of invasive NTS correlates with the clinical characteristics and bacteremia development in NTS infections remains unclear. In this study, we compared the global transcriptomes between bacteremic and nonbacteremic NTS strains after their interaction with human intestinal epithelial cells in vitro.

Published
2024
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