1. Metagenomic evidence for the microbial transformation of carboxyl-rich alicyclic molecules: A long-term macrocosm experiment
- Author
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Changfei He, Jihua Liu, Rui Wang, Yuanning Li, Qiang Zheng, Fanglue Jiao, Chen He, Quan Shi, Yongle Xu, Rui Zhang, Helmuth Thomas, John Batt, Paul Hill, Marlon Lewis, Hugh Maclntyre, Longfei Lu, Qinghua Zhang, Qichao Tu, Tuo Shi, Feng Chen, and Nianzhi Jiao
- Subjects
Diatoms ,Environmental Engineering ,Bacteria ,Ecological Modeling ,Microbiota ,Dissolved Organic Matter ,Pollution ,Phytoplankton ,Humans ,Metagenome ,Metagenomics ,Waste Management and Disposal ,Water Science and Technology ,Civil and Structural Engineering - Abstract
Carboxyl-rich alicyclic molecules (CRAMs) widely exist in the ocean and constitute the central part of the refractory dissolved organic matter (RDOM) pool. Although a consensus has been reached that microbial activity forms CRAMs, the detailed molecular mechanisms remain largely unexplored. To better understand the underlying genetic mechanisms driving the microbial transformation of CRAM, a long-term macrocosm experiment spanning 220 days was conducted in the Aquatron Tower Tank at Dalhousie University, Halifax, Canada, with the supply of diatom-derived DOM as a carbon source. The DOM composition, community structure, and metabolic pathways were characterised using multi-omics approaches. The addition of diatom lysate introduced a mass of labile DOM into the incubation seawater, which led to a low degradation index (I
- Published
- 2021