1. α-Tomatine inhibits growth and induces apoptosis in HL-60 human myeloid leukemia cells.
- Author
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Huang H, Chen S, Van Doren J, Li D, Farichon C, He Y, Zhang Q, Zhang K, Conney AH, Goodin S, Du Z, and Zheng X
- Subjects
- Animals, Body Weight drug effects, Cholesterol pharmacology, Female, HL-60 Cells, Humans, Leukemia, Myeloid drug therapy, Leukemia, Myeloid metabolism, Leukemia, Myeloid pathology, Mice, Mice, SCID, NF-kappa B metabolism, Tetradecanoylphorbol Acetate pharmacology, Tomatine chemistry, Tomatine pharmacology, Tomatine therapeutic use, Transplantation, Heterologous, Apoptosis drug effects, Cell Proliferation drug effects, Tomatine analogs & derivatives
- Abstract
α‑Tomatine is a glycoalkaloid that occurs naturally in tomatoes (Lycopersicon esculentum). In the present study, the effects of α‑tomatine on human myeloid leukemia HL‑60 cells were investigated. Treatment of HL‑60 cells with α‑tomatine resulted in growth inhibition and apoptosis in a concentration‑dependent manner. Tomatidine, the aglycone of tomatine had little effect on the growth and apoptosis of HL‑60 cells. Growth inhibition and apoptosis induced by α‑tomatine in HL‑60 cells was partially abrogated by addition of cholesterol indicating that interactions between α‑tomatine and cell membrane‑associated cholesterol may be important in mediating the effect of α‑tomatine. Activation of nuclear factor‑κB by the phorbol ester, 12‑O‑tetradecanoylphorbol‑13‑acetate failed to prevent apoptosis in HL‑60 cells treated with α‑tomatine. In animal experiments, it was found that treatment of mice with α‑tomatine inhibited the growth of HL‑60 xenografts in vivo. Results from the present study indicated that α‑tomatine may have useful anti‑leukemia activities.
- Published
- 2015
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