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The paper describes specific usage scenarios of an innovative platform, interfacing users, monitoring, and simulations for building energy simulations and the computation of key performance indicators to support, in an interoperable and open vision, design and management choices exploiting the enabling capabilities of ICT in architecture. The modularity of the proposed solution allows the development of pre-defined usage scenarios for professionals: impact of modifications in technological design choices, model calibration, and performance gap between simulated and monitored building data. The paper faces some new architectural usage scenarios of the tool, considering its enabling capabilities, and focuses on the tool's components developed and tested in the EU H2020 E-DYCE project. [ABSTRACT FROM AUTHOR]

Several tools and pieces of software support building energy modelling for optimization, certification and comparisons of different scenarios and usages. Nevertheless, the consistent rise in accessible computational power and the expansion of ICT are pushing the development of new software functionalities and tools able to support cross-disciplinary work on smart building optimization. This paper introduces a new platform (under development) that combines the EnergyPlus dynamic simulation tool with extra-functionalities and pre-defined usage scenarios based on automatic actions to manage massive simulations and correlation analyses. The tool's utility was tested in three experiments, with goals that we consider to be fundamental requirements: comparing simple retrofit actions to reduce net energy needs; analyzing the free-running potential of a demo building and the impacts of different low-energy technologies in terms of increasing thermal comfort (shading and ventilative cooling); and comparing measured sensor data indicators with simulated ones under real weather conditions for model verification. The results demonstrate the ability of the tool to automatically generate hundreds of EnergyPlus input building models by acting on building geometry; we focused on the most common uses for parametric dynamic simulations. Additionally, the way in which the tool combines the automatic modification of the building's design and the parallel launching of multiple simulations allows the labor to be reduced. The user can execute complex tasks without spending any time working with model editing software and aggregating the results from multiple simulations. [ABSTRACT FROM AUTHOR]

Abstract: The reaction between ozone and the three cresol isomers was investigated in pure water. Cresols were selected as model substrates representing an important component of humic material. Cresols carry both a hydroxyl and a methyl group, each theoretically increasing the reactivity of ozone with the aromatic ring. Direct comparison of the aromatic ring and the methyl group reactivities was made possible by the analysis of reaction products. The substrate degradation kinetics was studied by preparing aqueous solutions of each cresol and treating them with ozone for increasing time periods. It had been hypothesized that hydroxybenzaldehydes and hydroxybenzoic acids could be possible degradation intermediates of cresols. To verify this hypothesis, the degradation kinetics of three hydroxybenzaldehydes and two hydroxybenzoic acids were also studied. The reaction products were studied using gas chromatography (GC)-electron capture negative ionization (ECNI)-mass spectrometry (MS) analysis after direct derivatization of the samples with 5-chloro-2,2,3,3,4,4,5,5-octafluoro-1-pentyl chloroformate (ClOFPCF). This new analytical approach enables the extraction and analysis of highly polar polycarboxylic and hydroxycarboxylic acids, as well as highly polar aldehydes and hydroxy aldehydes that are difficult to extract and measure using conventional methods. As such, this new approach offered insights into ozone reaction intermediates that had been previously hypothesized, but not confirmed. Several highly hydrophilic degradation intermediates were identified, including malic, citraconic, itaconic, malonic, methylmuconic, and tartronic acid, but no hydroxybenzaldehydes were observed. The results support a 3-stage mechanism previously hypothesized, which involves ring-opening of the phenolic group, followed by the generation of several intermediates of increasing oxidation state, finally leading to relatively stable products, such as malonic and oxalic acids. We demonstrated that oxidation of the methyl group does not occur during cresol degradation. [Copyright &y& Elsevier]

An occurrence study was conducted to measure five iodoacids (iodoacetic acid, bromoiodoacetic acid, (Z)-3-bromo-3-iodo-propenoic acid, (E)-3-bromo-3-iodo-propenoic acid, and (E)-2-iodo-3-methylbutenedioic acid) and two iodo-trihalomethanes (iodo-THMs), (dichloroiodomethane and bromochloroiodomethane) in chloraminated and chlorinated drinking waters from 23 cities in the United States and Canada. Since iodoacetic acid was previously found to be genotoxic in mammalian cells, the iodo-acids and iodo-THMs were analyzed for toxicity. A gas chromatography (GC)/negative chemical ionizationmass spectrometry (MS) method was developed to measure the iodo-acids; iodo-THMs were measured using GC/high resolution electron ionization-MS with isotope dilution. The iodoacids and iodo-THMs were found in waters from most plants, at maximum levels of 1.7 μg/L (iodoacetic acid), 1.4 μg/L (bromoiodoacetic acid), 0.50 μg/L ((2)-3-bromo-3-iodopropenoic acid), 0.28 μg/L ((E)-3-bromo-3-iodopropenoic acid), 0.58 μg/L ((E)-2-iodo-3-methylbutenedioic acid), 10.2 μg/L (bromochloroiodomethane), and 7.9 μg/L (dichloroiodomethane). lodo-acids and iodo-THMs were highest at plants with short free chlorine contact times (<1 mm), and were lowest at a chlorineonly plant or at plants with long free chlorine contact times (>45 min). Iodide levels in source waters ranged from 0.4 to 104.2 μg/L (when detected), but there was not a consistent correlation between bromide and iodide. The rank order for mammalian cell chronic cytotoxicity of the compounds measured in this study, plus other iodinated compounds, was iodoacetic acid > (E)-3-bromo-2-iodopropenoic acid > iodoform > (E)-3-bromo-3-iodo-propenoic acid > (Z)-3-bromo-3-iodo-propenoic acid > diiodoacetic acid > bromoiodoacetic acid > (E)-2-iodo-3-methylbutenedioic acid > bromodiiodomethane > dibromoiodomethane > bromochloroiodomethane ≈ chlorodiiodomethane > dichloroiodomethane. With the exception of iodoform, the iodo-THMs were much less cytotoxic than the ado-acids. Of the 13 compounds analyzed, 7 were genotoxic; their rank order was iodoacetic acid ⪢ diiodoacetic acid > chlorodiiodomethane > bromoiodoacetic acid > E-2-iodo-3-methylbutenedioic acid > (E)-3-bromo-3-iodo-propenoic acid > (E)-3-bromo-2-iodopropenoic acid. In general, compounds that contain an iodo-group have enhanced mammalian cell cytotoxicity and genotoxicity as compared to their brominated and chlorinated analogues. [ABSTRACT FROM AUTHOR]

Several recent reports describe a role of probiotics as a therapeutic approach for celiac disease (CD). Two undigested A-gliadin peptides, P31-43 and P57-68, are central to CD pathogenesis, inducing an innate and an adaptive immune response, respectively. They enter enterocytes and localize to vesicular compartment to induce their toxic/immunogenics effects. In this article, we tested the effect of probiotic Lactobacillus paracasei (LP) CBA L74 (International Depository Accession Number LMG P-24778), its supernatant and LP-fermented cereals on gliadin peptides, P31-43 and P57-68, entrance in Caco-2 cells. Both LP CBA L74 and its supernatant inhibit P31-43 (intensity of fluorescence; FI: 75%) and P57-68 (FI: 50%) entrance in Caco2 cells, indicating that this biological effect is due to some product included in LP CBA L74 supernatant. This effect was present also after fermentation of cereals. This study describes a novel effect of probiotics in the prevention of undigested gliadin peptides toxic effects. [ABSTRACT FROM AUTHOR]

The rapid expansion of commercially available fermented food products raises important safety issues particularly when infant food is concerned. In many cases, the activity of the microorganisms used for fermentation as well as what will be the immunological outcome of fermented food intake is not known. In this manuscript we used complex in vitro, ex-vivo and in vivo systems to study the immunomodulatory properties of probiotic-fermented products (culture supernatant and fermented milk without live bacteria to be used in infant formula). We found in vitro and ex-vivo that fermented products of Lactobacillus paracasei CBA L74 act via the inhibition of proinflammatory cytokine release leaving anti-inflammatory cytokines either unaffected or even increased in response to Salmonella typhimurium. These activities are not dependent on the inactivated bacteria but to metabolic products released during the fermentation process. We also show that our in vitro systems are predictive of an in vivo efficacy by the fermented products. Indeed CBA L74 fermented products (both culture medium and fermented milk) could protect against colitis and against an enteric pathogen infection (Salmonella typhimurium). Hence we found that fermented products can act via the inhibition of immune cell inflammation and can protect the host from pathobionts and enteric pathogens. These results open new perspectives in infant nutrition and suggest that L. paracasei CBA L74 fermented formula can provide immune benefits to formula-fed infants, without carrying live bacteria that may be potentially dangerous to an immature infant immune system. [ABSTRACT FROM AUTHOR]

Background: Reduced CD4 T lymphocytes counts can be observed in HIV infection and in patients undergoing autologous haematopoietic stem cell transplantation (ASCT). Nevertheless, whereas opportunistic infections (OI) are frequent in HIVinfected individuals with low cell counts, OI are uncommon in ASCT patients. Methodology/Principal Findings: To verify whether this observation could be secondary to intrinsic HIV-correlated T cell defects, we performed in-depth immunologic analyses in 10 patients with comparable CD4 counts in whom lymphopenia was secondary either to HIV-infection or ASCT-associated immunosuppressive therapy and compared them to age-matched healthy subjects. Results showed the presence of profound alterations in CD4+ T lymphocytes in both groups of patients with respect to healthy controls. Thus, a low percentage of CCR7+ CD4+ T cells and a compensative expansion of CD45RA-CCR72 CD4+ T cells, a reduced IL-2/IFN-γ cytokine production and impaired recall antigens-specific proliferative responses were detected both in ASCT and HIV patients. In stark contrast, profound differences were detected in CD8+ T-cells between the two groups of patients. Thus, mature CD8+ T cell prevailed in ASCT patients in whom significantly lower CD45RA-CCR7- cells, higher CD45RA+CCR7- CD8+ cells, and an expansion of CCR7+CD8+ cells was detected; this resulted in higher IFN-γ +/TNFα production and granzyme CD8+ expression. The presence of strong CD8 T cells mediated immune responses justifies the more favorable clinical outcome of ASCT compared to HIV patients. Conclusion/Significance: These results indicate that CD8 T cells maturation and functions can be observed even in the face of a profound impairment of CD4+ T lymphocytes in ASCT but not in HIV patients. Primary HIV-associated CD8 defects or an imprinting by an intact CD4 T cell system in ASCT could justify these results. [ABSTRACT FROM AUTHOR]

The haloacetamides, a class of emerging nitrogenous drinking water disinfection byproduct (DBPs), were analyzed for their chronic cytotoxicity and for the induction of genomic DNA damage in Chinese hamster ovary cells. The rank order for cytotoxicity of 13 haloacetamides was DIAcAm > lAcAm > BAcAm > TBAcAm > BlAcAm > DBCAcAm > ClAcAm> BOCAcAm > OBAcAm > BCAcAm > CAcAm > OCAcAm > TCAcAm. The rank order of their genotoxicity was TBAcAm > DIAcAm ≈ lAcAm > BAcAm > DBCAcAm > BlAcAm > BDCAcAm > ClAcAm > BCAcAm > DBAcAm > CAcAm > TCAcAm. OCAcAm was not genotoxic. Cytotoxicity and genotoxicity were primarily determined by the leaving tendency of the halogens and followed the order I > Br >> Cl. With the exception of brominated trihaloacetamides, most of the toxicity rank order was consistent with structure-activity relationship expectations. For di- and trihaloacetamides, the presence of at least one good leaving halogen group (I or Br but not Cl) appears to be critical for significant toxic activity. Log P was not a factor for monohaloacetamides but may play a role in the genotoxicity of trihaloacetamides and possible activation of dihaloacetamides by intracellular GSH and -SH compounds. With the advent of the U.S. EPA Stage 2 DBP regulations, water utilities are considering the use of disinfectants that are alternatives to chlorine, The use of these afternative disinfectants will shiftthe distribution of DBP chemical classes. The emergence of new, highly toxic iodinated, nitrogenous DBPs, as illustrated by the discovery of bromoiodoacetamide as a new DBP, underscores the importance of comparative toxicity studies to assist in the overall goal of safer drinking water practice. [ABSTRACT FROM AUTHOR]

Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G (APOBEC3G), a human cytidine deaminase, is a potent inhibitor of HIV replication. To explore a possible role of this protein in modulating in vivo susceptibility to HIV infection, we analyzed APOBEC3G expression in HIV exposed seronegative individuals, HIV-seropositive patients, and healthy control subjects. The results showed that the expression of APOBEC3G is significantly increased in peripheral blood mononuclear cells (PBMCs)—mainly CD14+ cells—and in cervical tissues of HIV-exposed seronegative individuals. Higher APOBEC3G expression correlated with a reduced susceptibility of PBMCs to in vitro infection with the HIV-1Ba-L R5 strain. APOBEC3G could be important in modulating in vivo susceptibility to sexually transmitted HIV infection. [ABSTRACT FROM AUTHOR]

Abstract: We investigated the apoptosis of myelin basic protein (MBP)-specific T lymphocytes in multiple sclerosis (MS) patients with acute (AMS) or stable (SMS) MS by evaluating the expression of apoptosis markers on peripheral cells. Cells of healthy controls (HC) were evaluated as well. Results showed that mitogen-stimulated apoptosis was comparable among patients and controls, whereas MBP-stimulated CD4+ and CD8+7-AAD+ and 7-AAD+ Fas+ cell (apoptotic cells) were significantly reduced in AMS patients. A reduction of the apoptotic rate of myelin-specific CD4+ and CD8+T lymphocytes could be involved in the immune-mediated destruction of the myelin sheath seen in AMS patients. [Copyright &y& Elsevier]

In the pathogenesis of Alzheimer disease (AD), it has been proposed that the anti-inflammatory interleukins such as IL-10 regulate β-amyloid-induced microglial inflammatory responses inhibiting the proinflammatory cytokine IL-6. Since the promoters of the IL-10 and IL-6 genes show single nucleotide polymorphisms (SNPs) (IL-10: -1082 G→A; IL-6: -174 G→C), we investigated these SNPs and cytokine production by peripheral blood mononuclear cells in 65 AD patients and 65 controls (HC). In AD there was a significant increase of the -1082A IL-10 allele (P=0.009) and a decrease of -1082GG genotype (P=0.019). The frequency of the GG IL-6 genotype in AD was lower and the C allele significantly higher (P<0.005). The co-occurrence of IL-10 A and IL-6 C alleles significantly raised the odds ratio (OR 11.2, confidence interval: CI 1.3–97.3; P<0.05) independently of apolipoprotein E4 (adjusted OR 10.3, CI 1–108; P<0.05). Only amyloid-stimulated IL-10 production differed between the groups (P=0.023). These results raise questions regarding the inflammatory theory in AD, pointing to a pivotal role of IL-10 and IL-6 and a selective alteration in this network. [Copyright &y& Elsevier]

Analyzes the human alpha defensin in HIV-exposed but uninfected individuals. CD8+ T-lymphocyte purification; Production of alpha defensin; Flow cytometric analysis for alpha defensin; RNA extraction; Reverse transcription; Immunochemistry for alpha defensin detection; In situ alpha defensin detection.