166 results on '"Fasciana T."'
Search Results
2. T2 magnetic resonance (T2MR®) technology for managment of patients with sepsis
- Author
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Fasciana, T., primary, Amico, S., additional, Carelli, V., additional, Bona, M.R.M., additional, Ilardo, M., additional, Serra, N., additional, Di Carlo, P., additional, Campanile, F., additional, Giammanco, A., additional, and Andriolo, M., additional more...
- Published
- 2024
- Full Text
- View/download PDF
Catalog
3. The microbiota of the bilio-pancreatic system: a cohort, STROBE-compliant study
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Di Carlo P, Serra N, D'Arpa F, Agrusa A, Gulotta G, Fasciana T, Rodolico V, Giammanco A, and Sergi C
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Human Bile microorganisms ,Survival ,Pancreatic and Biliary Tract Disease ,E. Coli ,Infectious and parasitic diseases ,RC109-216 - Abstract
Paola Di Carlo,1 Nicola Serra,2 Francesco D’Arpa,3 Antonino Agrusa,3 Gaspare Gulotta,3 Teresa Fasciana,1 Vito Rodolico,1 Anna Giammanco,1 Consolato Sergi4,51Department of Sciences for Health Promotion, Mother & Child Care, University of Palermo, Palermo, Italy; 2Statistic Unit, Department of Public Health, University of Naples ‘Federico II’, Naples, Italy; 3Department of General Surgery and Emergency, University of Palermo, Palermo, Italy; 4Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada; 5Department of Laboratory Medicine and Pathology, Stollery Children’s Hospital, University of Alberta, Edmonton, AB, CanadaBackground: The gut microbiota play an essential role in protecting the host against pathogenic microorganisms by modulating immunity and regulating metabolic processes. In response to environmental factors, microbes can hugely alter their metabolism. These factors can substantially impact the host and have potential pathologic implications. Particularly pathogenic microorganisms colonizing pancreas and biliary tract tissues may be involved in chronic inflammation and cancer evolution.Purpose: To evaluate the effect of bile microbiota on survival in patients with pancreas and biliary tract disease (PBD).Patients and Methods: We investigated 152 Italian patients with cholelithiasis (CHL), cholangitis (CHA), cholangiocarcinoma (CCA), gallbladder carcinoma (GBC), pancreas head carcinoma (PHC), ampullary carcinoma (ACA), and chronic pancreatitis (CHP). Demographics, bile cultures, therapy, and survival rates were analyzed in cohorts (T1 death more...
- Published
- 2019
4. The EBV-DNA Can be Used as a Diagnostic and Follow-up Parameter of the Rhinopharyngeal Tumors in the Non-Endemic Population of the Western Sicily
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Lorusso, F., Caleca, M. P., Bellavia, C., Pistoia, D., Gallina, S., Speciale, R., Dispenza, F., Fasciana, T., and Capra, G.
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- 2019
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5. Fourth case of louse-borne relapsing fever in Young Migrant, Sicily, Italy, December 2015. Mini Review Article
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Colomba, C., Scarlata, F., Di Carlo, P., Giammanco, A., Fasciana, T., Trizzino, M., and Cascio, A.
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- 2016
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6. The influence of harvest period and fruit ripeness at harvest on minimally processed cactus pears (Opuntia ficus-indica L. Mill.) stored under passive atmosphere
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Allegra, A., Sortino, G., Miciletta, G., Riotto, M., Fasciana, T., and Inglese, P.
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- 2015
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7. Characterization of Acinetobacter baumannii from intensive care units and home care patients in Palermo, Italy
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Mammina, C., Bonura, C., Aleo, A., Calà, C., Caputo, G., Cataldo, M.C., Benedetto, A. Di, Distefano, S., Fasciana, T., Labisi, M., Sodano, C., Palma, D.M., and Giammanco, A.
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- 2011
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8. VALUTATION OF HUMAN β-DEFENSIN 2 AND 3 IN SERA OF HELICOBACTER PYLORI INFECTED OR NON-INFECTED PATIENTS: Abstract no.: P05.06
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Donnarumma, G., Fasciana, T., Fusco, A., Calà, C., Bonura, C., Scarpulla, G., and Giammanco, A.
- Published
- 2013
9. ANTIBIOTIC RESISTANCE PROFILES IN HELICOBACTER PYLORI STRAINS ISOLATED IN SICILY (ITALY): Abstract no.: P07.22
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Fasciana, T., Calà, C., Bonura, C., Di Carlo, E., Marineo, S., Scarpulla, G., Scarpulla, M., and Giammanco, A.
- Published
- 2011
10. REVISITING ANTHROPOZOONOSES IN THE MEDITERRANEAN BASIN. A SINGLE-CENTRE PERSPECTIVE. A SOUTHERN ITALIAN EXPERIENCE
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Guadagnino, G., Fasciana, T., Caputo, V., Scarlata, F., Giammanco, A., Paola Di Carlo, and Guadagnino G., Fasciana T., Caputo V., Scarlata F., Giammanco A., Di Carlo P.
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Anthropozoonoses, Mediterranean basin, Sicily - Abstract
Zoonoses, often better defined with the term anthropozoonoses, are diseases that can be transmitted to humans either by direct contact with animals or through arthropod vector intervention. Microbial interaction between humans and animals constitutes an important public health challenge, particularly in the Mediterranean basin. The main reasons this challenge is still relevant today include the phenomenon of migration, of both humans and animals, and climate change, which tend to alter the geographical distribution of zoonosis or the zoonotic agent, as well as the distribution range of potential vectors. The Mediterranean area seems to be affected by plentiful and widely distributed zoonoses, the main diseases being rickettsiosis, leishmaniasis, brucellosis, hydatid disease and viral zoonoses. The aim of this study is to revisit the prevalence and main clinical features of anthropozoonoses observed at the Department of Sciences for Health Promotion and Mother & Child Care, University of Palermo, Sicily, Southern Italy. more...
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- 2017
11. Bactibiliaindiseasesofthebiliarytractandpancreaticglandinpatients older than 80 years: a STROBE-retrospective cohort study in a teaching hospital in Italy
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DI CARLO, P., Nicola, S., Gulotta, G., Giammanco, A., Colomba, C., Melfa, G., Fasciana, T., and Consolato, S.
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Elderly .Bactibilia .Survivaltime .Gram-negativebacteria - Published
- 2018
12. A NEW CASE OF LOUSE-BORNE RELAPSING FEVER IN SICILY: CASE REPORT AND MINI REVIEW
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Fasciana, T., Calà, C., Colomba, C., Mascarella, C., Scarlata, F., Giuseppina CAPRA, Di Carlo, P., Giammanco, A., Fasciana, T., Cala', C., Colomba, C., Mascarella, C., Scarlata, F., Capra, G., DI CARLO, P., and Giammanco, A. more...
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B. recurrentis, Louse-borne Relapsing Fever, migrants - Abstract
Body lice transport B. recurrentis from man to man and humans are the only host. The presence of lice in Italy and an increasing number of cases in migrants can contribute to the onset of autochthonous cases. In this paper, we report a new case of Louse-borne Relapsing Fever (LBRF) diagnosed among migrants in Sicily exactly one year after the first case was recorded. We reviewed all cases reported in Europe from February 2016 until now. Our study identified two new cases of LBRF in migrants arrived in Europe: one who came from Somalia and one from Mali. Here we report data on a new case in Sicily. The number of migrants and refugees to transit in Sicily has increased, and this has led to the introduction of infectious diseases. Therefore, in our opinion it is essential to upgrade control of the sanitation conditions of migrants. more...
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- 2017
13. VIRULENCE FACTORS AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI ST131 IN COMMUNITY-ONSET HEALTHCARE-ASSOCIATED INFECTIONS IN SICILY, ITALY
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Fasciana, T., Giordano, G., Paola Di Carlo, Colomba, C., Mascarella, C., Tricoli, M. R., Calà, C., Giammanco, A., Fasciana, T., Giordano, G., DI CARLO, P., Colomba, C., Mascarella, C., Tricoli, M., Cala', C., and Giammanco, A. more...
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Escherichia coli, urinary tract infections, antibiotics, fluoroquinolones, cephalosporins - Abstract
Escherichia coli ST131 is an emerging resistant agent recently called “superbug” in England. This strain is responsible of community-acquired urinary tract infections and nowadays showing increasing resistance to antibiotics like fluoroquinolones and cephalosporins. Survey of virulent bacterial clone is relevant to control its spreading in community. We aim to assess the circulation of resistant clones Escherichia coli ST131 outside of the hospital to prompt control of outbreak in our geographical area. We selected 105 E. coli resistant isolates from community-acquired urinary infections and performed a multiplex PCR to evaluate if they belonged to the ST131 type. We investigated their set of virulence factors; in particular, kpsMII, papA, sfaS, focG, iutA, papC, hlyD and afa genes, and finally, we evaluated beta lactamases genes and quinolone resistance determinants. E. coli ST 131 clone was present in 66.6% of our isolates and showed positivity to a wide range of resistance genes, in particular blaCTX-M-15 among beta lactamases and plasmid-related quinolone resistance genes (qnrA, qnrS and aac (6’)-Ib-cr). Moreover, 81% of the strains showed positivity to at least one of the virulence factor genes. Our results suggested a high presence of E. coli ST131 in community. We suggest antibiotic stewardship for outpatient clinicians and facilities to contain the spread of “superbug” agents. more...
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- 2017
14. The EBV-DNA Can be Used as a Diagnostic and Follow-up Parameter of the Rhinopharyngeal Tumors in the Non-Endemic Population of the Western Sicily
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Lorusso, F., primary, Caleca, M. P., additional, Bellavia, C., additional, Pistoia, D., additional, Gallina, S., additional, Speciale, R., additional, Dispenza, F., additional, Fasciana, T., additional, and Capra, G., additional more...
- Published
- 2018
- Full Text
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15. Biofilm production in Staphylococcus epidermidis strains, isolated from the skin of hospitalized patients: genetic and phenotypic characteristics
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Calà, C., Amodio, E., Di Carlo, E., Virruso, R., Fasciana, T., Giammanco, A., Calà, C., Amodio, E., Di Carlo, E., Virruso, R., Fasciana, T., and Giammanco, A
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Microbiology (medical) ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,Cross Infection ,Genotype ,Biofilm ,Methicillin resistance ,Staphylococcal Infections ,S. epidermidis ,Skin ,Hospitalization ,Phenotype ,S. epidermidi ,Bacterial Proteins ,Biofilms ,Staphylococcus epidermidis ,Humans - Abstract
A major virulence factor of Staphylococcus epidermidis is its ability to form biofilms, permitting it to adhere to a surface and, in turn, to form a mucoid layer on polymer surfaces. Multiple factors have been found to influence bacterial attachment. Currently, this bacterium is commonly associated with hospital infections as a consequence of its ability to colonize, albeit accidentally, medical devices. This study investigated the genetic and phenotypic formation of biofilm in 105 S. epidermidis strains isolated from the skin of hospitalized patients. Fifty-eight of these patients were positive for the mecA gene (MRSE) and 47 were found to be negative (MSSE). Genetic characterizations were performed for the detection of the mecA, icaADBC, atlE, aap, bhp, IS256 and agr groups by PCR. Biofilm production was examined by culturing the strains in TBS medium and TBS with 0.5 and 1% respectively of glucose, and a semiquantitative assay on tissue culture plates was used. Although a molecular analysis estimate of detailed biofilm formation is costly in terms of time and complexity, a semiquantitative assay can be proposed as a rapid and cheap diagnostic method for initial screening to discover virulent strains. We confirmed a close correlation between genetic and phenotypic characteristics, highlighting the fact that, when S. epidermidis isolates were cultured in TSB with 1% of glucose, an increase in biofilm production was observed, as confirmed by positivity for the ica locus by molecular analysis. more...
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- 2014
16. Is the monoclonal spread of the ST258, KPC-3-producing clone being replaced in southern Italy by the dissemination of multiple clones of carbapenem-nonsusceptible, KPC-3-producing Klebsiella pneumoniae?
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Geraci, D.M., Bonura, C., Giuffrè, M., Saporito, L., Graziano, G., Aleo, A., Fasciana, T., Di Bernardo, F., Stampone, T., Palma, D.M., and Mammina, C.
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- 2015
- Full Text
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17. What Healthcare Workers Should Know about Environmental Bacterial Contamination in the Intensive Care Unit
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Russotto, V, Cortegiani, A, Fasciana, T, Iozzo, P, Raineri, S, Gregoretti, C, Giammanco, A, Giarratano, A, Raineri, SM, Russotto, V, Cortegiani, A, Fasciana, T, Iozzo, P, Raineri, S, Gregoretti, C, Giammanco, A, Giarratano, A, and Raineri, SM more...
- Abstract
Intensive care unit- (ICU-) acquired infections are a major health problem worldwide. Inanimate surfaces and equipment contamination may play a role in cross-transmission of pathogens and subsequent patient colonization or infection. Bacteria contaminate inanimate surfaces and equipment of the patient zone and healthcare area, generating a reservoir of potential pathogens, including multidrug resistant species. Traditional terminal cleaning methods have limitations. Indeed patients who receive a bed from prior patient carrying bacteria are exposed to an increased risk (odds ratio 2.13, 95% confidence intervals 1.62-2.81) of being colonized and potentially infected by the same bacterial species of the previous patient. Biofilm formation, even on dry surfaces, may play a role in reducing the efficacy of terminal cleaning procedures since it enables bacteria to survive in the environment for a long period and provides increased resistance to commonly used disinfectants. No-touch methods (e.g., UV-light, hydrogen peroxide vapour) are under investigation and further studies with patient-centred outcomes are needed, before considering them the standard of terminal cleaning in ICUs. Healthcare workers should be aware of the role of environmental contamination in the ICU and consider it in the broader perspective of infection control measures and stewardship initiatives more...
- Published
- 2017
18. Panton-Valentine leukocidin positive sequence type 80 methicillin-resistant Staphylococcus aureus carrying a staphylococcal cassette chromosome mec type IVc is dominant in neonates and children in an Algiers hospital
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Djoudi, F., Bonura, C., Benallaoua, S., Touati, A., Touati, D., Aleo, A., Cala, C., Fasciana, T., Caterina Mammina, Djoudi F, Bonura C, Benallaoua S, Touati A, Touati D, Aleo A, Cala C, Fasciana T, and Mammina C. more...
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Methicillin-Resistant Staphylococcus aureus ,Cross Infection ,Molecular Epidemiology ,Adolescent ,Bacterial Toxins ,Exotoxins ,Infant ,Microbial Sensitivity Tests ,Staphylococcal Infections ,Settore MED/42 - Igiene Generale E Applicata ,Polymerase Chain Reaction ,Bacterial Typing Techniques ,Electrophoresis, Gel, Pulsed-Field ,Community-Acquired Infections ,MRSA, PVL, CA-MRSA, ST80, neonates, children ,Leukocidins ,Algeria ,Child, Preschool ,Humans ,Child ,Multilocus Sequence Typing - Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a major antimicrobial drug-resistant pathogen causing serious infections. It was first detected in healthcare settings, but in recent years it has also become disseminated in the community. Children and young adults are most susceptible to infection by community-acquired (CA) MRSA strains. In this study 25 MRSA isolates implicated in infections of neonates and children admitted to an Algiers hospital during an 18 month period were characterized by molecular methods including staphylococcal cassette chromosome (SCC) mec typing, PCR amplification of pvl genes, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Fifteen out of 25 isolates were from hospital-acquired infections. Twenty-four isolates carried SCCmec type IVc and belonged to the sequence type (ST) 80, one isolate carried SCCmec type II and was ST 39. Twenty-two out of 24 ST80-MRSA-IVc isolates carried pvl genes. Our results suggest that the Panton-Valentine leukocidin positive ST80- MRSA-IVc is the dominant MRSA clone causing disease in neonates and children in Algiers. more...
- Published
- 2012
19. Rapidly growing mycobacteria in TB/HIV co-infection: a report of two cases focusing on difficulties in diagnosis and management
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Bonura, C., Di Carlo, P., Spicola, D., Calà, C., Caterina Mammina, Fasciana, T., Giammanco, A., Bonura, C, Di Carlo, P, Spicola, D, Calà, C, Mammina, C, Fasciana, T, and Giammanco, A
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Settore MED/07 - Microbiologia E Microbiologia Clinica ,Settore MED/17 - Malattie Infettive ,RGM, TB/HIV coinfection, Diagnosis - Abstract
Recent reports indicate an increase in rates of infection and disease due to rapidly growing mycobacteria (RGM) in patients with pre-existing chronic lung disease. Studies have described difficulties in correctly identifying closely related species, even when proper methodologies are adopted, and several different gene targets have been proposed. We describe two cases of RGM infection in a 29-year-old HIV-1 positive Congolese man and a 19-year-old HIV-1 positive Liberian woman, respectively, both with bronchiectasis due to previous Mycobacterium tuberculosis (MTB) infection. Mycobacterium porcinum and Mycobacterium bolletii were identified in bronchoalveolar lavage fluid and sputum, respectively. After starting the patients on antiretroviral treatment and primary prophylaxis against non-tuberculous mycobacteria (NTM), and ensuring that they adhered to their prescribed regimen, we observed an improvement in their clinical condition and mycobacteria cleared from their respiratory specimens. Management of RGM respiratory infection in immunocompromised patients has to be evaluated on a case-by-case basis, taking into account the patient's pulmonary sequelae, adherence to multiple treatments and immune profile. more...
- Published
- 2012
20. Antibiotic resistance profiles in Helicobacter pylori strains isolated in Sicily (Italy)
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Fasciana, T., Cala, C., Bonura, C., Di Carlo, E., Sandra Marineo, Scarpulla, G., Scarpulla, M., Giammanco, A., Fasciana, T, Calà, C, Bonura, C, Di Carlo, E, Marineo,S, Scarpulla, G, Scarpulla, M, and Giammanco, A more...
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Settore MED/07 - Microbiologia E Microbiologia Clinica ,Antibiotic Resistance ,H.pylori - Published
- 2011
21. Isolation, identification and oenological characterization of non-Saccharomyces yeasts in a Mediterranean island
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Polizzotto, G., primary, Barone, E., additional, Ponticello, G., additional, Fasciana, T., additional, Barbera, D., additional, Corona, O., additional, Amore, G., additional, Giammanco, A., additional, and Oliva, D., additional more...
- Published
- 2016
- Full Text
- View/download PDF
22. Programma di sorveglianza attiva per la tubercolosi in Sicilia
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Mammina, C, Bonura, C, Aleo, A, Fasciana, T, Ghodousi, A, and Agodi, ANTONELLA PAOLA
- Published
- 2014
23. Sherris - Microbiologia Medica
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Giammanco, A., Cala', C., Fasciana, T., Anna Giammanco, Cinzia Calà, and Teresa Fasciana
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Microbiologia medica - Abstract
Il testo di Sherris “Medical Microbiology”, giunto oggi alla sua sesta edizione, continua a rappresentare, a livello internazionale, un testo cardine per lo studio della Microbiologiamedica sia nel Corso di Medicina e Chirurgia sia nell’ambito della formazionespecialistica. La prima edizione di questo testo venne pubblicata nel 1976 da John C. Sherris, Professore Emerito di Microbiologia, noto per gli studi sulle resistenze batteriche agli antibioticied il rapido evolversi delle Scienze Mediche ed in particolare della Microbiologia ha resonecessari numerosi aggiornamenti che hanno portato alla attuale sesta edizione. Abbiamo partecipato con entusiasmo alla prima edizione italiana e con altrettanto entusiasmopartecipiamo alla seconda, sempre convinti del valore didattico del testo. Il libro è adatto a studenti che frequentano corsi di Microbiologia di base, poiché fornisce una descrizione completa e dettagliata degli agenti di infezione: classificazione, struttura, replicazion e malattia, ma anche a studenti che frequentano corsi di Microbiologia Clinica, in quanto tratta di diagnosi, prevenzione e terapia. Un rinnovato ringraziamento all’Editore Dr. Antonio Diomaiuta la cui stima ed amicizia ha reso il lavoro di noi tutti agevole ed efficiente. more...
- Published
- 2012
24. Ongoing spread of colistin-resistant Klebsiella pneumoniae in different wards of an acute general hospital, Italy, June to December 2011
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Mammina, C, primary, Bonura, C, additional, Di Bernardo, F, additional, Aleo, A, additional, Fasciana, T, additional, Sodano, C, additional, Saporito, M A, additional, Verde, M S, additional, Tetamo, R, additional, and Palma, D M, additional more...
- Published
- 2012
- Full Text
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25. Helicobacter pylori and epstein–barr co-infection in gastric disease
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Fasciana, T., Giuseppina CAPRA, Calà, C., Zambuto, S., Mascarella, C., Colomba, C., Di Carlo, P., Giammanco, A., Fasciana, T., Capra, G., Cala', C., Zambuto, S., Mascarella, C., Colomba, C., DI CARLO, P., and Giammanco, A. more...
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H. pylori, EBV, coinfection, Italy, gastric diseases - Abstract
The incidence of gastrointestinal diseases and in particular gastric cancer (GC) is high worldwide. Over the last few years, numerous studies have speculated that Helicobacter pylori (H. pylori) and Epstein-Barr virus (EBV) can be correlated with gastric cancerogenesis. Virulence factors of H. pylori can contribute to the variability of clinical outcomes: among the most important virulence factors is the pathogenicity island (CagPAI), vacA and oipA gene. EBV infection usually persists in B cells and induces an inflammatory reaction in cooperation with H. pylori. In Sicily, H. pylori and EBV infections are particularly prevalent, and to our knowledge no study has addressed this yet. The aim of our study was to examine the association of H. pylori and EBV infection in patients with gastric diseases in Sicily. Gastric biopsies were collected from 24 adult patients with chronic gastritis active (CGA) and from 24 adult patients without any gastric disease (NGD) who underwent upper gastrointestinal endoscopy. H. pylori infection was diagnosed by PCR for ureaseA gene while EBV-DNA was detected by Real time PCR for region Bam HI-W. Moreoever, we investigated the presence of CagPaI and the status of vacA and oipA genes. Percentage of resistance to Clarithromycin of H. pylori was evaluated also. We established that H. pylori and EBV infection was present in 42% of patients, while dual infection with H. pylori and EBV-DNA was present in 54% of the patients with CGA. In patients with NGD we found that H. pylori and EBV infection was present in 46% and in 21% of patients respectively, while co-infection was present in 33% of patients. CagPAI was present in only 20% of patients with GCA and in 9% of patients with NGD. As regards vacA alleles, s2i2m2 were predominant, present in 80% and 82% of patients with CGA and NGD respectively. The status “ON” of oipA gene was present in the same percentage. Finally, we found that 38% of patients positive for H. pylori infection showed resistance to Clarithromycin. In our study, there was a strong association between the simultaneous presence of H. pylori and EBV infection in patients with CGA compared to patients with NGD. Furthermore, our data confirmed the high percentage of resistance among H. pylori strains circulating in Sicily, underlining the importance of establishing a therapy that is effective in eradicating them and reducing the frequency of coinfections and evolution towards gastric cancerogenesis more...
26. Coinfection of Helicobacter pylori and Epstein-Barr virus in patients with gastric disease in Southern Italy
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Mascarella, C., CAPRA, Giuseppina, Fasciana, Teresa Maria Assunta, CALA', Cinzia, Immordino, R., Fasciana, T., PITARRESI, Giovanna Laura, Scarpulla, G., and GIAMMANCO, Anna
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Settore MED/07 - Microbiologia E Microbiologia Clinica ,Helicobacter pylori ,Epstein-Barr - Abstract
Helicobacter pylori
27. What Healthcare Workers Should Know about Environmental Bacterial Contamination in the Intensive Care Unit
- Author
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Tma Fasciana, Andrea Cortegiani, Cesare Gregoretti, Santi Maurizio Raineri, Antonino Giarratano, Pasquale Iozzo, Russotto, Anna Giammanco, Russotto, V., Cortegiani, A., Fasciana, T., Iozzo, P., Raineri, S., Gregoretti, C., Giammanco, A., Giarratano, A., Russotto, V, Cortegiani, A, Fasciana, T, Iozzo, P, Raineri, S, Gregoretti, C, Giammanco, A, and Giarratano, A more...
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Health Personnel ,030106 microbiology ,lcsh:Medicine ,Review Article ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,03 medical and health sciences ,law ,Long period ,Health care ,medicine ,Humans ,Infection control ,Terminal cleaning ,MED/41 - ANESTESIOLOGIA ,Intensive care medicine ,Decontamination ,Cross Infection ,Infection Control ,Healthcare, Environmental, Bacterial, Contamination, Intensive Care Unit ,General Immunology and Microbiology ,business.industry ,lcsh:R ,General Medicine ,Contamination ,Intensive care unit ,Intensive Care Units ,Increased risk ,Equipment Contamination ,Environmental Pollution ,business ,Human - Abstract
Intensive care unit- (ICU-) acquired infections are a major health problem worldwide. Inanimate surfaces and equipment contamination may play a role in cross-transmission of pathogens and subsequent patient colonization or infection. Bacteria contaminate inanimate surfaces and equipment of the patient zone and healthcare area, generating a reservoir of potential pathogens, including multidrug resistant species. Traditional terminal cleaning methods have limitations. Indeed patients who receive a bed from prior patient carrying bacteria are exposed to an increased risk (odds ratio 2.13, 95% confidence intervals 1.62–2.81) of being colonized and potentially infected by the same bacterial species of the previous patient. Biofilm formation, even on dry surfaces, may play a role in reducing the efficacy of terminal cleaning procedures since it enables bacteria to survive in the environment for a long period and provides increased resistance to commonly used disinfectants. No-touch methods (e.g., UV-light, hydrogen peroxide vapour) are under investigation and further studies with patient-centred outcomes are needed, before considering them the standard of terminal cleaning in ICUs. Healthcare workers should be aware of the role of environmental contamination in the ICU and consider it in the broader perspective of infection control measures and stewardship initiatives. more...
- Published
- 2017
- Full Text
- View/download PDF
28. Factors leading to dissemination of cutaneous anthrax: an international ID-IRI study
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U.S. Elbahr, R. Tekin, M. Papić, N. Pandak, H. Erdem, F.K. Can, S.N. Alpat, A.U. Pekok, F. Pehlivanoglu, M. Karamese, P.C. Petru, S. Kulzhanova, S. Tosun, M. Doğan, R. Moroti, E. Ramosaco, H. Alay, E. Puca, J. Arapovic, N. Pshenichnaya, T. Fasciana, A. Giammanco, Elbahr U.S., Tekin R., Papic M., Pandak N., Erdem H., Can F.K., Alpat S.N., Pekok A.U., Pehlivanoglu F., Karamese M., Petru P.C., Kulzhanova S., Tosun S., Dogan M., Moroti R., Ramosaco E., Alay H., Puca E., Arapovic J., Pshenichnaya N., Fasciana T., and Giammanco A. more...
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Anthrax ,Gastrointestinal ,Meningiti ,Infectious Diseases ,Outcome ,Meningitis ,Bacteremia ,Mortality ,Microbiology - Abstract
Background: Although anthrax is a rare zoonotic infection, it still causes significant mortality and morbidity. In this multicenter study, which is the largest anthrax case series ever reported, we aimed to describe the factors leading to dissemination of cutaneous anthrax.Methods: Adult patients with cutaneous anthrax from 16 referral centers were pooled. The study had a retrospective design, and included patients treated between January 1, 1990 and December 1, 2019. Probable, and confirmed cases based upon CDC anthrax 2018 case definition were included in the study. A descriptive statistical analysis was performed for all variables.Results: A total of 141 cutaneous anthrax patients were included. Of these, 105 (74%) patients had probable and 36 (26%) had confirmed diagnosis. Anthrax meningitis and bacteremia occurred in three and six patients, respectively. Sequelae were observed in three patients: cicatricial ectropion followed by ocular anthrax (n = 2) and movement restriction on the left hand after surgical intervention (n = 1). One patient had gastrointestinal anthrax. The parameters related to poor outcome (p < 0.05) were fever, anorexia, hypoxia, malaise/fatigue, cellulitis, fasciitis, lymphadenopathy, leukocytosis, high CRP and creatinine levels, longer duration of antimicrobial therapy, and combined therapy. The last two were seemingly the consequences of dissemination rather than being the reasons. The fatality rate was 1.4%.Conclusions: Rapid identification of anthrax is crucial for prompt and effective treatment. Systemic symptoms, disseminated local infection, and high inflammatory markers should alert the treating physicians for the dissemination of the disease. (c) 2022 The Authors. Published by Elsevier Ltd. more...
- Published
- 2022
29. Sexually Transmitted Diseases: Diagnosis and Control
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Teresa Fasciana, Giuseppina Capra, Dario Lipari, Alberto Firenze, Anna Giammanco, Fasciana T., Capra G., Lipari D., Firenze A., Giammanco A., Fasciana, Teresa, Capra, Giuseppina, Lipari, Dario, Firenze, Alberto, and Giammanco, Anna more...
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Diseases ,Risk Factors ,Sexual Behavior ,Health, Toxicology and Mutagenesis ,Transmitted ,Sexually Transmitted Diseases ,Public Health, Environmental and Occupational Health ,Humans ,Control ,Sexually ,STD ,Diagnosi - Abstract
Sexually transmitted diseases (STDs)—or sexually transmitted infections (STIs)—are generally acquired through sexual contact [...]
- Published
- 2022
30. Survival in rhino-orbito-cerebral mucormycosis: An international, multicenter ID-IRI study
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Yasemin Cag, Hakan Erdem, Mehmet Gunduz, Suheyla Komur, Handan Ankarali, Serap Ural, Meltem Tasbakan, Pierre Tattevin, Anil Tombak, Derya Ozturk-Engin, Ayse Sagmak Tartar, Ayse Batirel, Recep Tekin, Fazilet Duygu, Hulya Caskurlu, Behice Kurtaran, Bulent Durdu, Demet Haciseyitoglu, Jordi Rello, Fatma Sirmatel, Antonio Cascio, Botond Lakatos, Serkan Oncu, Ayse Willke, Edmond Puca, Meliha Meric Koc, Pinar Korkmaz, Oguz Karabay, Rahmet Guner, Selmin Dirgen Caylak, Sema Keceli, Salih Cesur, Sirin Menekse, Pinar Ergen, Mehmet Sezai Tasbakan, Ayşegül Erdem, Yakup Cag, Emine Akcay, Teresa Fasciana, Anna Giammanco, Rabia Burçin Girgin, Canan Agalar, Haluk Vahaboglu, Istanbul Medeniyet University (IMU), Ankara University School of Medicine [Turkey], Cukurova University, Izmir Katip Celebi University (IKCU), Ege university, CHU Pontchaillou [Rennes], ARN régulateurs bactériens et médecine (BRM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Mersin University, Firat University, Dicle University, Vall d’Hebron Research Institute (VHIR), Centre Hospitalier Universitaire de Nîmes (CHU Nîmes), None, Cag Y., Erdem H., Gunduz M., Komur S., Ankarali H., Ural S., Tasbakan M., Tattevin P., Tombak A., Ozturk-Engin D., Tartar A.S., Batirel A., Tekin R., Duygu F., Caskurlu H., Kurtaran B., Durdu B., Haciseyitoglu D., Rello J., Sirmatel F., Cascio A., Lakatos B., Oncu S., Willke A., Puca E., Koc M.M., Korkmaz P., Karabay O., Guner R., Caylak S.D., Keceli S., Cesur S., Menekse S., Ergen P., Tasbakan M.S., Erdem A., Akcay E., Fasciana T., Giammanco A., Girgin R.B., Agalar C., and Vahaboglu H. more...
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Adult ,Antifungal Agents ,Surveillance ,Neutropenia ,Epidemiology ,Mucormycosi ,Infections ,Hospital-acquired infection ,Rhino-orbito-cerebral mucormycosis ,Zygomycosis ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Debridement ,Risk Factors ,Diagnosis ,Orbital Diseases ,Internal Medicine ,Humans ,Mucormycosis ,Rhino-orbito-cerebral mucormycosi ,Eye Infections, Fungal ,Retrospective Studies - Abstract
International audience; BACKGROUND: Mucormycosis is an emerging aggressive mold infection. This study aimed to assess the outcome of hospitalized adults with rhino-orbito-cerebral mucormycosis (ROCM). The secondary objective was to identify prognostic factors in this setting. METHODS: This study was an international, retrospective, multicenter study. Patients’ data were collected from 29 referral centers in 6 countries. All qualified as "proven cases" according to the EORTC/MSGERC criteria. RESULTS: We included 74 consecutive adult patients hospitalized with ROCM. Rhino-orbito-cerebral type infection was the most common presentation (n = 43; 58.1%) followed by rhino-orbital type (n = 31; 41.9%). Twenty (27%) had acquired nosocomial bacterial infections. A total of 59 (79.7%) patients (16 in combination) received appropriate antifungal treatment with high-doses of liposomal amphotericin B. Fifty-six patients (75.7%) underwent curative surgery. Thirty-five (47.3%) required intensive care unit admission (27; 36.5% under mechanical ventilation). Hospital survival was 56.8%, being reduced to 7.4% in patients with invasive mechanical ventilation. A multivariate binary backward logistic regression model identified confusion at admission (OR 11.48), overlapping hospital-acquired infection (OR 10.27), use of antifungal treatment before diagnosis (OR 10.20), no surgical debridement (OR 5.92), and the absence of prior sinusitis (OR 6.32) were independently associated with increased risk for death. CONCLUSION: Today, ROCM still has high mortality rate. Improving source control, rational therpy, and preventing nosocomial infections may improve survival in this severe infection. more...
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- 2022
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31. Transfusion‐transmitted malaria of plasmodium malariae in palermo, sicily
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Maria Rita Tricoli, Orazia Diquattro, Maria Favarò, Antonio Cascio, Anna Giammanco, Teresa Fasciana, Maurizio Musso, Jessica Pulvirenti, Natascia Oliveri, Pulvirenti J., Musso M., Fasciana T., Cascio A., Tricoli M.R., Oliveri N., Favaro M., Diquattro O., and Giammanco A. more...
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Pediatrics ,medicine.medical_specialty ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,Blood transfusion ,Leadership and Management ,Anemia ,medicine.medical_treatment ,malaria ,Case Report ,thrombocytopenia ,Health Informatics ,Plasmodium malariae ,Parasitemia ,blood transfusion ,blood donor screening ,Asymptomatic ,transfusion-transmitted malaria ,Health Information Management ,Asymptomatic semi‐immune donors, Blood donor screening, Blood transfusion, Malaria, Plasmodium malariae, Thrombocytopenia, Transfusion-transmitted malaria ,parasitic diseases ,medicine ,biology ,business.industry ,Health Policy ,Incidence (epidemiology) ,biology.organism_classification ,medicine.disease ,Diagnosis of malaria ,asymptomatic semi-immune donors ,Medicine ,medicine.symptom ,business ,Malaria - Abstract
Transfusion-transmitted malaria (TTM) is a rare occurrence with serious consequences for the recipient. In non-endemic areas, the incidence of transmission of malaria by transfusion is very low. We report a clinical case of transfusion-transmitted malaria due to Plasmodium malariae, which happened in a patient with acute hemorrhagic gastropathy. Case presentation: In April 2019, a 70-year-old Italian man with recurrent spiking fever for four days was diagnosed with a P. malariae infection, as confirmed using microscopy and real-time PCR. The patient had never been abroad, but about two months before, he had received a red blood cell transfusion for anemia. Regarding the donor, we revealed that they were a missionary priest who often went to tropical regions. Plasmodium spp. PCR was also used on donor blood to confirm the causal link. Discussion and Conclusions: The donations of asymptomatic blood donors who are predominantly “semi-immune” with very low parasitic loads are an issue. The main problem is related to transfusion-transmitted malaria. Our case suggests that P. malariae infections in semi-immune asymptomatic donors are a threat to transfusion safety. Currently, microscopy is considered the gold standard for the diagnosis of malaria but has limited sensitivity to detect low levels of parasitemia. Screening using serological tests and molecular tests, combined with the donor’s questionnaire, should be used to reduce the cases of TTM. more...
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- 2021
32. Epidemiology and Pattern of Resistance of Gram-Negative Bacteria Isolated from Blood Samples in Hospitalized Patients: A Single Center Retrospective Analysis from Southern Italy
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Sofia Lo Sauro, Alessandro Lucchesi, Paola Di Carlo, Anna Giammanco, Juan Camilo Signorello, Nicola Serra, Teresa Fasciana, Consolato Sergi, Maria Santa Napolitano, Teresa Rea, Vincenza Maria Carelli, Giuseppe Manta, Antonio Cascio, Maurizio Giarratana, Di Carlo P., Serra N., Sauro S.L., Carelli V.M., Giarratana M., Signorello J.C., Lucchesi A., Manta G., Napolitano M.S., Rea T., Cascio A., Sergi C.M., Giammanco A., Fasciana T., Di Carlo, Paola, Serra, Nicola, Lo Sauro, Sofia, Carelli, Vincenza Maria, Giarratana, Maurizio, Signorello, Juan Camilo, Lucchesi, Alessandro, Manta, Giuseppe, Napolitano, Maria Santa, Rea, Teresa, Cascio, Antonio, Sergi, Consolato Maria, Giammanco, Anna, and Fasciana, Teresa more...
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Microbiology (medical) ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,Carbapenem ,medicine.medical_specialty ,medicine.drug_class ,Antibiotics ,Tigecycline ,RM1-950 ,Biochemistry ,Microbiology ,Article ,law.invention ,Antibiotic resistance ,law ,Internal medicine ,MDR ,medicine ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,bacteria ,survival time ,biology ,business.industry ,biology.organism_classification ,medicine.disease ,Intensive care unit ,infection ,Acinetobacter baumannii ,Infectious Diseases ,Bacteria, Infection, MDR, Survival time ,Bacteremia ,Colistin ,Therapeutics. Pharmacology ,business ,medicine.drug - Abstract
Background: Blood culturing remains the mainstream tool to inform an appropriate treatment in hospital-acquired bloodstream infections and to diagnose any bacteremia. Methods: A retrospective investigation on the prevalence of Gram-negative bacteria (GNB) and their resistance in hospitalized patients by age, sex, and units from blood cultures (BCs) was conducted from January 2018 to April 2020 at Sant’Elia hospital, Caltanissetta, southern Italy. We divided the patient age range into four equal intervals. Results: Multivariate demographic and microbiological variables did not show an association between bacteria distributions and gender and age. The distribution by units showed a higher prevalence of Klebsiella pneumoniae and Acinetobacter baumannii in the intensive care unit (ICU) and Escherichia coli in the non-intensive care units (non-ICUs). The analysis of antibiotic resistance showed that E. coli was susceptible to a large class of antibiotics such as carbapenem and trimethoprim-sulfamethoxazole. K. pneumoniae showed a significant susceptibility to colistin, tigecycline, and trimethoprim-sulfamethoxazole. From the survival analysis, patients with E. coli had a higher survival rate. Conclusions: The authors stress the importance of the implementation of large community-level programs to prevent E. coli bacteremia. K. pneumoniae and E. coli susceptibility patterns to antibiotics, including in the prescription patterns of general practitioners, suggest that the local surveillance and implementation of educational programs remain essential measures to slow down the spread of resistance and, consequently, increase the antibiotic lifespan. more...
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- 2021
33. Potential Activity of Albino Grifola frondosa Mushroom Extract against Biofilm of Meticillin-Resistant Staphylococcus aureus
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Maria Letizia Gargano, Orazia Diquattro, Anna Giammanco, Salvatore Vieni, Nicola Serra, Teresa Fasciana, Giuseppa Graceffa, Giuseppe Venturella, Elena Galia, Maria Rita Tricoli, Ignazio Arrigo, Fasciana, T, Gargano, ML, Serra, N, Galia, E, Arrigo, I, Tricoli, MT, Diquattro, O, Graceffa, G, Vieni, S, Venturella, G, and Giammanco, A more...
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Microbiology (medical) ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,Grifola frondosa ,QH301-705.5 ,Staphylococcus aureus ,Plant Science ,medicine.disease_cause ,biofilm ,03 medical and health sciences ,Functional food ,medicine ,Food science ,Biology (General) ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,activities ,0303 health sciences ,Mushroom ,biology ,030306 microbiology ,Chemistry ,business.industry ,Biofilm ,biochemical phenomena, metabolism, and nutrition ,Antimicrobial ,biology.organism_classification ,Food safety ,Settore BIO/03 - Botanica Ambientale E Applicata ,Staphylococcus aureu ,business ,Bacteria - Abstract
Mushroom extracts are a rich source of natural compounds with antimicrobial properties, which are able to prevent, to some extent, the growth of foodborne pathogens. The aim of this study was to investigate the potential of extracts from albino Grifola frondosa (GF), commonly known as maitake, to inhibit the growth of some bacteria and the biofilm production by Staphylococcus aureus. We obtained not only a significant reduction of OD score between biofilm and biofilm plus albino G. frondosa extract group, but also a reduction of category of biofilm. In addition, we observed a significant presence of isolates with strong category for the biofilm group and a significant presence of isolates with absent category for the biofilm plus albino G. frondosa extract group. These results confirm that the use of albino G. frondosa extract reduces in significant way the presence of biofilm. Our results suggest and confirm that albino G. frondosa extracts could be employed as functional food and could be used as a natural additive for food process control and food safety. more...
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- 2021
34. Spread of Multidrug-Resistant Microorganisms
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Silvia Di Lodovico, Teresa Fasciana, Mara Di Giulio, Luigina Cellini, Anna Giammanco, Gian Maria Rossolini, Alberto Antonelli, Di Lodovico S., Fasciana T., Giulio M.D., Cellini L., Giammanco A., Rossolini G.M., and Antonelli A. more...
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Microbiology (medical) ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,Infectious Diseases ,bacteria ,Pharmacology (medical) ,MDRO ,General Pharmacology, Toxicology and Pharmaceutics ,Biochemistry ,Microbiology - Abstract
Multidrug-resistant organisms (MDRO) are bacteria that exhibit acquired resistance to multiple antibiotics, reducing the efficacy of antimicrobial therapies [...]
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- 2022
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35. Draft genome sequence and biofilm production of a carbapenemase-producing Klebsiella pneumoniae (KpR405) sequence type 405 strain isolated in Italy
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Teresa Fasciana, Daniela Maria Palma, Roberta Virruso, Bernardina Gentile, Andrea Ciammaruconi, Giovanna Pitarresi, Maria Rita Tricoli, Paola Di Carlo, Anna Giammanco, Florigio Lista, Fasciana T., Ciammaruconi A., Gentile B., Di Carlo P., Virruso R., Tricoli M.R., Palma D.M., Pitarresi G.L., Lista F., and Giammanco A. more...
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0301 basic medicine ,Microbiology (medical) ,Klebsiella pneumoniae ,030106 microbiology ,Virulence ,RM1-950 ,Biochemistry ,Microbiology ,Carbapenemase ,03 medical and health sciences ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Pathogen ,Whole genome sequencing ,biology ,Biofilm ,Outbreak ,ST405 ,biology.organism_classification ,030104 developmental biology ,Infectious Diseases ,Multilocus sequence typing ,Therapeutics. Pharmacology ,Bacteria - Abstract
Rapid identification and characterization of multidrug-resistant Klebsiella pneumoniae strains is essential to diagnose severe infections in patients. In clinical routine practice, K. pneumoniae is frequently identified and characterized for outbreak investigation. Pulsed-field gel electrophoresis or multilocus sequence typing could be used, but, unfortunately, these methods are time-consuming, laborious, expensive, and do not provide any information about the presence of resistance and virulence genes. In recent years, the decreasing cost of next-generation sequencing and its easy use have led to it being considered a useful method, not only for outbreak surveillance but also for rapid identification and evaluation, in a single step, of virulence factors and resistance genes. Carbapenem-resistant strains of K. pneumoniae have become endemic in Italy, and in these strains the ability to form biofilms, communities of bacteria fixed in an extracellular matrix, can defend the pathogen from the host immune response as well as from antibiotics, improving its persistence in epithelial tissues and on medical device surfaces. more...
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- 2021
36. Potential impact of a nonavalent anti HPV vaccine in Italian men with and without clinical manifestations
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Liana Bosco, Leonardo Di Gregorio, Gloria Calagna, Alberto Firenze, Rosaria Schillaci, Daniela Pistoia, Nicola Serra, Antonino Perino, Teresa Fasciana, Marco Vella, Giuseppina Capra, Bosco L., Serra N., Fasciana T., Pistoia D., Vella M., Di Gregorio L., Schillaci R., Perino A., Calagna G., Firenze A., and Capra G. more...
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Hpv genotypes ,Sexually transmitted disease ,Adult ,Male ,medicine.medical_specialty ,Science ,Diseases ,Microbiology ,Article ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Medical research ,Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18 ,Internal medicine ,Medicine ,Humans ,Human papilloma virus infection ,030212 general & internal medicine ,Papillomavirus Vaccines ,Papillomaviridae ,Sicily ,Aged ,Potential impact ,Multidisciplinary ,business.industry ,HPV Positive ,Papillomavirus Infections ,Health care ,Middle Aged ,030220 oncology & carcinogenesis ,Case-Control Studies ,business ,Sexual contact ,HPV, nonavalent vaccine, men, infectious desease - Abstract
Human papilloma virus infection (HPV) is the most common sexually transmitted disease. Little is known about male infection. Nonavalent vaccine against types 6/11/16/18/31/33/45/52/58 was approved and neutral gender immunization programs have been proposed. This study evaluates the potential impact of nonavalent vaccine compared to quadrivalent in male living in Sicily (Italy). 58.7% of samples were HPV positive and forty-four types of HPV were identified. A significant higher estimated coverage of nonavalent vaccine than quadrivalent was observed (64.3% vs. 45.8%), with absolute and relative additional impact of 20.1% and 47.2%, respectively. Low impact of the vaccine were calculated as the empirical probability of HPV genotypes 6/11/16/18/31/33/45/52/58 alone or in combination; the high impact as empirical probability of HPV6/11/16/18/31/33/45/52/58 genotypes alone or in association with other genotypes. The potential impact of the nonavalent vaccine vs quadrivalent was significant for low and high impact (29.7% > 18:8%; 34:6% > 26.6%, respectively). Particularly, in men with lesions and risky sexual contact was significant only for low impact (35.5% > 29.7%; 31.4% > 19.7%, respectively). In partners with positive females was significant for low impact (26.3% > 15.1%) and high impact (33.7% > 23.2%). Nonavalent vaccine offers broader protection in men with HPV positive partners, who would have a potential role in the transmission of the infection. more...
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- 2021
37. Socio-Demographic Characteristics and Sexual Behavioral Factors of Patients with Sexually Transmitted Infections Attending a Hospital in Southern Italy
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Marco Vella, Giuseppina Capra, Teresa Fasciana, Giuseppe Pistone, Cinzia Calà, Paola Di Carlo, Anna Giammanco, Claudia Colomba, Fasciana T., Capra G., Di Carlo P., Cala' C., Vella M., Pistone G., Colomba C., and Giammanco A. more...
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Adult ,Male ,HPV ,Sexual Behavior ,Health, Toxicology and Mutagenesis ,Socio demographics ,Sexually Transmitted Diseases ,syphilis ,HIV Infections ,Mycoplasma genitalium ,STIs ,Article ,Sexual and Gender Minorities ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,medicine ,Global health ,Humans ,Mycoplasma Infections ,030212 general & internal medicine ,MSM ,Homosexuality, Male ,Sicily ,Demography ,Retrospective Studies ,behaviour risky factors, HPV, MSM, STIs, syphilis ,030505 public health ,Chlamydia ,biology ,business.industry ,behaviour risky factors ,Public Health, Environmental and Occupational Health ,HPV infection ,virus diseases ,Retrospective cohort study ,medicine.disease ,biology.organism_classification ,Hospitals ,Sexual Partners ,Sexual behavior ,Medicine ,Female ,Syphilis ,0305 other medical science ,business - Abstract
Sexually transmitted infections (STIs) are a serious global health problem. In Italy, data describing the vulnerability to STIs of specific sexual minorities and the influence of sociodemographic and behavioral determinants are limited, as most infections are not subject to mandatory notification. This retrospective study describes the sociodemographic profile and main sexual behaviors of patients attending a hospital in Palermo (Sicily, Italy) from January 2018 to March 2019 as predictors of STI risk. Patients were divided in subgroups: men-who-have-sex-with-men (MSM), men-who-have-sex-with-women (MSW), bisexual men and females. Data were obtained through an anonymous questionnaire. Patients were tested for chlamydia, syphilis, Mycoplasma genitalium infection, genital herpes and HPV infection. A total of 294 subjects with STIs (male/female ratio about 2:1) were screened. Of the total sample, 79.6% of patients were Italian. MSM accounted for 34.3%, MSW for 29.6%, bisexual men for 5.8% and females for 30.3%. A total of 44.5% of patients had a high education level, 42.5% reported irregular use of condoms, 20.7% reported having had 5–10 partners in the six months prior to the visit and 32.9% were HIV-positive. HPV infection and syphilis were the most prevalent STIs. Conclusions: The most common profile of patients attending our clinic was that of an adult, Italian man with a high level of education, poor use of condoms and a high number of partners. MSM had the highest sex-behavior-related risk for STIs. In addition, our results suggest that all STD teams need to implement counselling topics and recommendations to share with patients, as well as tips on how to approach sexual health education/counselling, thereby promoting patient-centered approaches and educational programs. more...
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- 2021
38. Current and Future Trends in the Laboratory Diagnosis of Sexually Transmitted Infections
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Anna Giammanco, Roberta Virruso, Giorgia Caruso, Teresa Fasciana, Caruso G., Giammanco A., Virruso R., and Fasciana T.
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medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Point-of-care testing ,Gonorrhea ,Sexually Transmitted Diseases ,diagnostic ,lcsh:Medicine ,HIV Infections ,Review ,Diagnostic tools ,03 medical and health sciences ,0302 clinical medicine ,Prevalence ,medicine ,Humans ,NAAT ,Syphilis ,030212 general & internal medicine ,sexually transmitted infection ,immunoassay ,metagenomic ,Intensive care medicine ,sexually transmitted infections ,metagenomics ,0303 health sciences ,Trichomoniasis ,High prevalence ,Chlamydia ,Clinical Laboratory Techniques ,030306 microbiology ,business.industry ,Public health ,lcsh:R ,Public Health, Environmental and Occupational Health ,Chlamydia Infections ,culturomics ,medicine.disease ,culture ,microscopy ,point-of-care test ,biosensing ,business - Abstract
Sexually transmitted infections (STIs) continue to exert a considerable public health and social burden globally, particularly for developing countries. Due to the high prevalence of asymptomatic infections and the limitations of symptom-based (syndromic) diagnosis, confirmation of infection using laboratory tools is essential to choose the most appropriate course of treatment and to screen at-risk groups. Numerous laboratory tests and platforms have been developed for gonorrhea, chlamydia, syphilis, trichomoniasis, genital mycoplasmas, herpesviruses, and human papillomavirus. Point-of-care testing is now a possibility, and microfluidic and high-throughput omics technologies promise to revolutionize the diagnosis of STIs. The scope of this paper is to provide an updated overview of the current laboratory diagnostic tools for these infections, highlighting their advantages, limitations, and point-of-care adaptability. The diagnostic applicability of the latest molecular and biochemical approaches is also discussed. more...
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- 2021
39. International Multicentre Study of Candida auris Infections
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Teresa Fasciana, Nirav Pandya, Anna Giammanco, Hulya Caskurlu, Ilker Inanc Balkan, Yasemin Cag, Aruna Poojary, Yogesh Kumar Gupta, Ejaz Ahmed Khan, Folusakin Ayoade, Nenad Pandak, Abdullah Umut Pekok, Hakan Erdem, Dhanji P. Rajani, Pandya N., Cag Y., Pandak N., Pekok A.U., Poojary A., Ayoade F., Fasciana T., Giammanco A., Caskurlu H., Rajani D.P., Gupta Y.K., Balkan I.I., Khan E.A., and Erdem H. more...
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Microbiology (medical) ,Settore MED/07 - Microbiologia E Microbiologia Clinica ,medicine.medical_specialty ,Echinocandin ,C. auris, Candida, Fungi, Nosocomial, Outbreak, Resistance ,QH301-705.5 ,Plant Science ,Emergence ,resistance ,chemistry.chemical_compound ,C. auris ,Internal medicine ,Epidemiology ,medicine ,Infection control ,Biology (General) ,Ecology, Evolution, Behavior and Systematics ,Candida ,outbreak ,business.industry ,Mortality rate ,Micafungin ,nosocomial ,Resistant ,Management ,chemistry ,Candida auris ,Anidulafungin ,fungi ,Caspofungin ,business ,medicine.drug - Abstract
Background:Candida auris has emerged globally as a multi-drug resistant yeast and is commonly associated with nosocomial outbreaks in ICUs. Methods: We conducted a retrospective observational multicentre study to determine the epidemiology of C. auris infections, its management strategies, patient outcomes, and infection prevention and control practices across 10 centres from five countries. Results: Significant risk factors for C. auris infection include the age group of 61–70 years (39%), recent history of ICU admission (63%), diabetes (63%), renal failure (52%), presence of CVC (91%) and previous history of antibiotic treatment (96%). C. auris was commonly isolated from blood (76%). Echinocandins were the most sensitive drugs. Most common antifungals used for treatment were caspofungin (40%), anidulafungin (28%) and micafungin (15%). The median duration of treatment was 20 days. Source removal was conductedin 74% patients. All-cause crude mortality rate after 30 days was 37%. Antifungal therapy was associated with a reduction in mortality (OR:0.27) and so was source removal (OR:0.74). Contact isolation precautions were followed in 87% patients. Conclusions:C. auris infection carries a high risk for associated mortality. The organism is mainly resistant to most azoles and even amphotericin-B. Targeted antifungal therapy, mainly an echinocandin, and source control are the prominent therapeutic approaches. more...
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- 2021
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40. Infective Endocarditis: Preliminary Results of a Cohort Study in the Southern Italian Population
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Vincenzo Argano, Claudia Colomba, Paola Di Carlo, Giuseppina Novo, Anna Giammanco, Gabriele Palermo, Maria Michela Marino, Nicola Serra, Teresa Fasciana, Consolato Sergi, Teresa Rea, Serra, N, Colomba, C, Di Carlo, P, Palermo, G, Fasciana, T, Giammanco, A, Novo, G, Rea, T, Marino, MM, Argano, V, and Sergi, C more...
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microorganism ,medicine.medical_specialty ,Arterial embolism ,complications ,Cardiology ,univariate analysi ,candida endocarditi ,030204 cardiovascular system & hematology ,endocarditi ,03 medical and health sciences ,0302 clinical medicine ,matlab ,Internal medicine ,medicine ,gender ,Endocarditis ,Blood culture ,adult cardiac surgery ,microorganisms ,Surgical team ,Univariate analysis ,medicine.diagnostic_test ,business.industry ,General Engineering ,medicine.disease ,Cardiac surgery ,univariate analysis ,candida endocarditis ,multivariate analysis ,Infective endocarditis ,multi-drug resistant bacteria ,endocarditis ,business ,030217 neurology & neurosurgery ,Cohort study - Abstract
Background Infective endocarditis (IE) is an uncommon disease with an involved interplay of clinical and surgical team management. We aimed to define diagnosis parameters and delineate in-hospital management in patients with IE admitted in a tertiary hospital of Southern Italian. Materials and methods Fifty-six consecutive patients (42 males, 14 females; age range: 34-85 years) admitted for IE in the Infectious Diseases, Cardiac Surgery, and Cardiology units, between January 2011 and August 2017, were enrolled. Demographic data, mortality, comorbidities, specimen type, microscopy results, special histological staining performed, and antimicrobial therapy were collected and analyzed. Any comments at the multidisciplinary team meetings were recorded in minutes of and approved. Results We found 83.9% of patients with positive blood cultures. The four most common bacteria were methicillin-resistant Staphylococcus aureus (MRSA: 21.3%), methicillin-sensitive Staphylococcus aureus (MSSA: 17%), Streptococci (14.9%), and Enterococci (14.9%). Both in the univariate and multivariate analysis, we observed a significant positive correlation between surgery and complications. Particularly in the univariate analysis only, surgery was positively correlated to males and C-reactive protein (CPR) at baseline. Also, considering the most common bacteria, it resulted in a positive correlation between surgery and MRSA and Streptococci spp. and between complications and MSSA. Finally, the male gender was positively correlated to MSSA and heart complications, major arterial embolism, septic pulmonary emboli, splenic infarction, and cerebral embolism. Conclusions A blood culture test remains a critical factor for the diagnosis of IE and the antibiotic treatment of susceptible and emerging resistant bacteria. Male gender and heart complications are red flags for prompt operative management. more...
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- 2020
41. Ecology, Phylogeny, and Potential Nutritional and Medicinal Value of a Rare White 'Maitake' Collected in a Mediterranean Forest
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Georgios I. Zervakis, Teresa Fasciana, Giuseppe Venturella, Maria Letizia Gargano, Roberta Calvo, Valeria Ferraro, Anna Giammanco, Omoanghe S. Isikhuemhen, Gargano ML, Zervakis GI, Isikhuemhen OS, Venturella G, Calvo R, Giammanco A, Fasciana T, and Ferraro V more...
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0106 biological sciences ,Grifola frondosa ,bioprospecting ,Biology ,01 natural sciences ,03 medical and health sciences ,Dry weight ,Staphylococcus epidermidis ,Botany ,polypore fungu ,polypore fungus ,Mediterranean forest ,Internal transcribed spacer ,lcsh:QH301-705.5 ,Ribosomal DNA ,030304 developmental biology ,Nature and Landscape Conservation ,0303 health sciences ,Mushroom ,Ecology ,Settore BIO/02 - Botanica Sistematica ,Ecological Modeling ,Quercus pubescens ,ITS rDNA ,biology.organism_classification ,Grifola ,Agricultural and Biological Sciences (miscellaneous) ,medicinal mushroom ,fungal diversity ,phylogenetics ,lcsh:Biology (General) ,Settore BIO/03 - Botanica Ambientale E Applicata ,Basidiocarp ,phylogenetic ,010606 plant biology & botany ,basidiomycete - Abstract
Albino Grifola frondosa (Dicks.) Gray &ldquo, maitake&rdquo, mushrooms (described as G. albicans Imazeki and then placed in synonymy with G. frondosa) are particularly rare, and the few pertinent records are not treated in scientific publications. A field investigation carried out in Sicily (Italy) led to the collection of an unusual white Grifola specimen at the base of a living tree of Quercus pubescens Willd. s.l. The outcome of sequencing the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) indicated that it belongs to G. frondosa and provided an insight to the phylogenetic relationships within the genus. The results of nutritional composition analysis showed that the albino basidioma possesses relatively high contents of Ca, Fe, K, and Cu and is rather low in Na when compared with literature data on edible mushrooms. Vitamin (B1, B2, B3, B5, B9, and D2) contents ranged from 0.15 to 3.89 mg per 100 g of mushroom dry weight. The cold-water extract of this specimen was effective at inhibiting the growth of Staphylococcus epidermidis ATCC 12228 and Pseudomonas aeruginosa ATCC 15442 at the maximum screening concentration of 50% v/v. In addition, the extract slowed down the ability of Staphylococcus aureus ATCC 43300 to form biofilms. According to data hereby reported, the albino G. frondosa is a culinary-medicinal mushroom with a promising exploitation potential. more...
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- 2020
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42. The EBV-DNA Can be Used as a Diagnostic and Follow-up Parameter of the Rhinopharyngeal Tumors in the Non-Endemic Population of the Western Sicily
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Giuseppina Capra, Riccardo Speciale, F. Lorusso, Salvatore Gallina, Daniela Pistoia, Maria Pia Caleca, Teresa Fasciana, Carmelina Bellavia, Francesco Dispenza, and Lorusso, F., Caleca, M.P., Bellavia, C., Pistoia, D., Gallina, S., Speciale, R., Dispenza, F., Fasciana, T., Capra, G. more...
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Pathology ,medicine.medical_specialty ,education.field_of_study ,business.industry ,Population ,Nasopharyngeal neoplasm ,EBV Cancer of nasopharynx Nasopharyngeal carcinoma Nasopharyngeal neoplasm ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Blood serum ,medicine.anatomical_structure ,Otorhinolaryngology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Carcinoma ,medicine ,Original Article ,Surgery ,030223 otorhinolaryngology ,Rhinopharyngeal ,education ,business ,Viral load ,Lymph node - Abstract
To evaluate whether EBV-DNA can be used as a diagnostic and follow-up parameter for nasopharyngeal tumors in a non-endemic population. The study was carried out in a university hospital. A retrospective study was conducted on 40 paraffin samples of histological preparations. EB-DNA was detected by real-time PCR technique. A prospective study was also conducted on a group of 30 patients who underwent nasopharyngeal biopsy for suspected nasopharyngeal carcinoma (NPC) by comparing EBV-DNA concentrations between the histological specimen and the serum. Quantification of genomic copies of EBV-DNA in serum and detection of anti-EBV antibodies was performed. In both groups the presence of high viral load of EBV-DNA was found in nonkeratinizing squamous cell carcinomas, in three cases of lymphepitomyoma and in 4 out of 6 cases of non-differentiated non-carcinoma lymph node metastases. squamous keratinizing cells. In all cases of NPC, an antibody pattern typical of reactivations (IgGVCA+, IgG-EA+, IgG-EBNA+) and IgA-EA-D, frequently positive in cases of NPC, has been highlighted. A good correlation between the high EBV-DNA charges and the histological diagnosis was highlighted. Our study also found that the assessment of viral EBV load can also be considered in the prognostic evaluation and in the follow-up of patients with NPC. more...
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- 2018
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43. Cluster of Legionnaires’ Disease in an Italian Prison
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Cinzia Calà, Angela Rampulla, M Palermo, S. Distefano, Paola Di Carlo, Chiara Mascarella, Anna Giammanco, Giuseppina Capra, Teresa Fasciana, Fasciana T., Mascarella C., Distefano S.A., Cala C., Capra G., Rampulla A., Di Carlo P., Palermo M., and Giammanco A. more...
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System ,Veterinary medicine ,Legionella pneumophila ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,water ,lcsh:Medicine ,Prison ,030230 surgery ,Reference laboratory ,Disease cluster ,Article ,03 medical and health sciences ,0302 clinical medicine ,Prevalence ,medicine ,Cluster Analysis ,Humans ,Typing ,cluster ,Genotyping ,media_common ,0303 health sciences ,biology ,030306 microbiology ,lcsh:R ,Public Health, Environmental and Occupational Health ,Sequence types ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,respiratory tract diseases ,Italy ,Prisons ,bacteria ,systems ,Legionnaires' disease ,prison ,Legionnaires' Disease ,Water Microbiology ,Environmental Monitoring - Abstract
Background: Legionella pneumophila (Lp) is the most common etiologic agent causing Legionnaires&rsquo, Disease (LD). Water systems offer the best growth conditions for Lp and support its spread by producing aerosols. From 2015 to 2017, the Regional Reference Laboratory of Clinical and Environmental Surveillance of Legionellosis of Palermo monitored the presence of Lp in nine prisons in Western Sicily. During this investigation, we compared Lp isolates from environmental samples in a prison located in Palermo with isolates from two prisoners in the same prison. Methods: We collected 93 water samples from nine Sicilian prisons and the bronchoalveolar lavages (BALs) of two prisoners considered cases of LD. These samples were processed following the procedures described in the Italian Guidelines for the Prevention and Control of Legionellosis of 2015. Then, genotyping was performed on 19 Lp colonies (17 from water samples and 2 from clinical samples) using the Sequence-Based Typing (SBT) method, according to European Study Group for Legionella Infections (ESGLI) protocols. Results: Lp serogroup (sg) 6 was the most prevalent serogroup isolated from the prisons analyzed (40%), followed by Lp sg 1 (16%). Most of all, in four penitentiary institutions, we detected a high concentration of Lp >, 104 Colony Forming Unit/Liter (CFU/L). The environmental molecular investigation found the following Sequence Types (STs) in Lp sg 6: ST 93, ST 292, ST 461, ST 728, ST 1317 and ST 1362, while most of the isolates in sg 1 belonged to ST 1. We also found a new ST that has since been assigned the number 2451 in the ESGLI-SBT database. From the several Lp sg 1 colonies isolated from the two BALs, we identified ST 2451. Conclusions: In this article, we described the results obtained from environmental and epidemiological investigations of Lp isolated from prisons in Western Sicily. Furthermore, we reported the first cluster of Legionnaires&rsquo, in an Italian prison and the molecular typing of Lp sg 1 from one prison&rsquo, s water system and two BALs, identified the source of the contamination, and discovered a new ST. more...
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- 2019
44. Global Assessment of the Activity of Tigecycline against Multidrug-Resistant Gram-Negative Pathogens between 2004 and 2014 as Part of the Tigecycline Evaluation and Surveillance Trial
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Anna Giammanco, Cinzia Calà, Teresa Fasciana, Michael J. Dowzicky, Patricia A. Bradford, Giammanco, A., Calà, C., Fasciana, T., and Dowzicky, M.
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0301 basic medicine ,Klebsiella ,030106 microbiology ,lcsh:QR1-502 ,Tigecycline ,medicine.disease_cause ,030226 pharmacology & pharmacy ,Microbiology ,lcsh:Microbiology ,Clinical Science and Epidemiology ,surveillance studie ,03 medical and health sciences ,0302 clinical medicine ,multidrug resistance ,medicine ,surveillance studies ,Molecular Biology ,biology ,Pseudomonas aeruginosa ,Klebsiella oxytoca ,biology.organism_classification ,Antimicrobial ,QR1-502 ,Acinetobacter baumannii ,Multiple drug resistance ,Gram-negative bacteria ,tigecycline ,Enterobacter cloacae ,Research Article ,medicine.drug - Abstract
Multidrug resistance among bacterial pathogens is an ongoing global problem and renders antimicrobial agents ineffective at treating bacterial infections. In the health care setting, infections caused by multidrug-resistant (MDR) Gram-negative bacteria can cause increased mortality, longer hospital stays, and higher treatments costs. The aim of the Tigecycline Evaluation and Surveillance Trial (TEST) is to assess the in vitro antimicrobial activities of tigecycline and other contemporary agents against clinically relevant pathogens. This paper presents antimicrobial activity data from the TEST study between 2004 and 2014 and examines global rates of MDR Gram-negative isolates, including Acinetobacter baumannii, Pseudomonas aeruginosa, and members of the Enterobacteriaceae, during this time. Our results show that tigecycline retained in vitro activity against many MDR Gram-negative pathogens over the study period, while rates of MDR A. baumannii increased globally. Using these findings, we hope to highlight the current status of multidrug resistance in medical facilities worldwide., Multidrug-resistant (MDR) Gram-negative organisms are a burden on the global health care system. The Tigecycline Evaluation and Surveillance Trial (TEST) is an ongoing global study designed to monitor the in vitro activities of tigecycline and a panel of marketed antimicrobials against a range of clinically significant pathogens. In this study, in vitro data are presented for MDR Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter aerogenes, and Enterobacter cloacae isolates collected from 2004 to 2014. In total, 13% (21,967/170,759) of isolates displayed multidrug resistance globally, with the highest rates recorded among A. baumannii (overall rate, 44% [8,294/18,741], increasing from 23% [309/1,323] in 2004 to 63% [447/712] in 2014). Other multidrug resistance rates ranged from 2.5% for K. oxytoca (203/8,000) to 12% for P. aeruginosa and K. pneumoniae (3,951/32,786 and 3,895/32,888, respectively), and rates among these pathogens remained stable during the study period. Against MDR E. coli, Klebsiella spp., and E. aerogenes, the lowest rates of resistance were to tigecycline (0.2%, 6%, and 12%, respectively), and the lowest MIC90 value against A. baumannii was observed for tigecycline (2 mg/liter; MIC range, ≤0.008 to ≥32 mg/liter). The only significant change in resistance to tigecycline during the study period was for MDR E. coli (P < 0.01), among which eight resistant isolates were identified globally from 2009 to 2013. In summary, these results show that tigecycline retained in vitro activity against the majority of MDR Gram-negative organisms presented here, but the rising rates of MDR A. baumannii highlight the need for the continued monitoring of global multidrug resistance. IMPORTANCE Multidrug resistance among bacterial pathogens is an ongoing global problem and renders antimicrobial agents ineffective at treating bacterial infections. In the health care setting, infections caused by multidrug-resistant (MDR) Gram-negative bacteria can cause increased mortality, longer hospital stays, and higher treatments costs. The aim of the Tigecycline Evaluation and Surveillance Trial (TEST) is to assess the in vitro antimicrobial activities of tigecycline and other contemporary agents against clinically relevant pathogens. This paper presents antimicrobial activity data from the TEST study between 2004 and 2014 and examines global rates of MDR Gram-negative isolates, including Acinetobacter baumannii, Pseudomonas aeruginosa, and members of the Enterobacteriaceae, during this time. Our results show that tigecycline retained in vitro activity against many MDR Gram-negative pathogens over the study period, while rates of MDR A. baumannii increased globally. Using these findings, we hope to highlight the current status of multidrug resistance in medical facilities worldwide. more...
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- 2017
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45. Genotyping and Antifungal Susceptibility of Dipodascus capitatus Isolated in a Neonatal Intensive Care Unit of a Sicilian Hospital
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Dario Lipari, Giuseppa Pinello, Giovanni Corsello, Giuseppe Aquilina, Cinzia Calà, Giuseppina Capra, Teresa Fasciana, Anna Giammanco, Ingrid Anne Mandy Schierz, Mario Giuffrè, Fasciana, T., Giuffrè, M., Calà, C., Schierz, I., Aquilina, G., Pinello, G., Capra, G., Lipari, D., Corsello, G., and Giammanco, A more...
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0301 basic medicine ,Antifungal ,Genotyping ,Pediatrics ,medicine.medical_specialty ,Clonal dissemination ,Neonatal intensive care unit ,medicine.drug_class ,030106 microbiology ,Dipodascus ,03 medical and health sciences ,Epidemiology ,medicine ,Dipodascus capitatu ,Antifungal Susceptibility ,Dipodascus capitatus ,Biochemistry, Genetics and Molecular Biology (all) ,biology ,Medicine (all) ,Micafungin ,Nosocomial Acquisition ,Cross Infection ,Female ,Genotype ,Hospitals ,Humans ,Infant ,Infant, Newborn ,Intensive Care Units, Neonatal ,Male ,Mycoses ,Sicily ,biology.organism_classification ,medicine.drug - Abstract
In August 2015, Dipodascus capitatus was isolated from two patients admitted to the neonatal intensive care unit. Nosocomial acquisition of the fungus was suspected and epidemiological studies were undertaken. The patients were simultaneously hospitalized, and the comparison of the two isolates by two independent molecular typing methods have confirmed clonal dissemination of a single strain of D. capitatus. Antimicrobial susceptibility testing was useful for identifying the appropriated antifungal therapy in micafungin. To our knowledge these are the first described cases of neonatal D. capitatus infection and also the first report of successful treatment by micafungin. more...
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- 2017
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46. Potential impact of a nonavalent HPV vaccine on HPV related low-and high-grade cervical intraepithelial lesions: A referral hospital-based study in Sicily
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Giuseppina Capra, Alessandra Vassiliadis, Alberto Firenze, Domenica Matranga, Lucia Giovannelli, Giovanna Scaduto, Teresa Fasciana, Maria Francesca Guarneri, Carmelina Bellavia, Antonio Perino, Capra, G., Giovannelli, L., Matranga, D., Bellavia, C., Guarneri, M., Fasciana, T., Scaduto, G., Firenze, A., Vassiliadis, A., and Perino, A. more...
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Oncology ,Adult ,medicine.medical_specialty ,Referral ,Genotype ,Immunology ,Uterine Cervical Neoplasms ,HPV vaccines ,Cervix Uteri ,Hospital based study ,03 medical and health sciences ,0302 clinical medicine ,Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18 ,Internal medicine ,vaccine ,medicine ,Prevalence ,Humans ,Immunology and Allergy ,030212 general & internal medicine ,Papillomavirus Vaccines ,quadrivalent ,Referral and Consultation ,Sicily ,Female population ,squamous intraepithelial lesion ,Gynecology ,Pharmacology ,Potential impact ,Hpv types ,business.industry ,Papillomavirus Infections ,HPV infection ,squamous intraepithelial lesions ,virus diseases ,medicine.disease ,Uterine Cervical Dysplasia ,Research Papers ,impact estimate ,nonavalent ,030220 oncology & carcinogenesis ,Female ,business - Abstract
While bivalent and quadrivalent HPV vaccines have been used for about 10 years, a nonavalent vaccine against HPV types 6/11/16/18/31/33/45/52 and 58 has been recently approved by FDA and EMA and is now commercially available. The objective of our study was to evaluate the potential impact of the nonavalent vaccine on HPV infection and related low- and high-grade squamous intraepithelial lesions (LSIL, HSIL), compared to the impact of the quadrivalent vaccine, in a female population living in Sicily (Italy). Low estimates of HPV vaccine impact were calculated as prevalence of HPV 6/11/16/18/31/33/45/52 and 58 genotypes, alone or in association, but excluding presence of other HPV types; high estimates were calculated as prevalence of HPV 6/11/16/18/31/33/45/52 and 58 genotypes alone or in association, in the presence of other HPV types. The nonavalent HPV vaccine showed increased impact, compared to the quadrivalent vaccine. Estimates of potential impact varied from 30.9% (low estimate) to 53.3% (high estimate) for LSIL, and from 56.9% to 81,0% for HSIL. The proportion of additional cases potentially prevented by the nonavalent vaccine was 14.4%–23.8% for LSIL, and 19.0%–32.8% for HSIL. The benefit of the nonavalent vaccine compared to the quadrivalent vaccine was more than 80% for both low and high impact estimates for LSIL and more than 50% for both low and high impact estimates for HSIL. The present study confirms that the switch from a first generation HPV vaccines to a nonavalent vaccine would increase the prevention of cervical HSIL in up to 90% of cases. more...
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- 2017
47. Isolamento e tipizzazione di stipiti di Dipodascus capitatus: patogeno opportunista emergente
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Fasciana, Teresa Maria Assunta, CALA', Cinzia, BONURA, Celestino, CAPRA, Giuseppina, SEDDIO, Giovanna, CHIARAMONTE, Rosaria, GIAMMANCO, Anna, Immordino, R., Fasciana, T., Calà, C., Bonura, C., Capra, G., Seddio, G., Immordino, R., Chiaramonte, R., and Giammanco, A more...
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Settore MED/07 - Microbiologia E Microbiologia Clinica ,Dipodascus capitatus, isolamento, tipizzazione - Abstract
L’incidenza delle infezioni micotiche ha subito negli ultimi decenni un incremento rilevante, sia in ambito ospedaliero che comunitario. In ambiente ospedaliero il problema sta assumendo dimensioni preoccupanti poiché, sebbene Candida spp. si debba considerare il principale responsabile di infezioni correlate ai miceti, emergono altre specie il cui ruolo deve essere accertato. In particolare Dipodascus capitatus, forma teleomorfica di Geotrichum capitatum, microrganismo che può risiedere nel suolo, sulla pelle e nel tratto respiratorio e gastroenterico, nel passato considerato un contaminate o un innocuo colonizzatore, da qualche anno è stato associato ad infezioni soprattutto nei pazienti immunocompromessi. Sebbene le infezioni da tale microrganismo siano spesso favorite dall’uso di antibiotici e di cateteri, le forme invasive sembrano verificarsi in pazienti neutropenici e nei soggetti sottoposti a chemioterapia intensiva per leucemia acuta. Dai reparti ad alto rischio sono stati anche rilevati episodi epidemici D. capitatus-correlati. Il suo riscontro non deve quindi essere sottovalutato ed è necessario poter disporre di test semplici che ne permettano una rapida identificazione. Tra i metodi fenotipici quello che si basa sulla valutazione della crescita in terreno cromogeno è considerato ottimale, soprattutto se si utilizza il CCA (Chromogenic Candida Agar, Oxoid); ed è stato già descritto quale metodo molecolare il sequenziamento. Anche dai reparti dell’A.O.U.P. “P. Giaccone” di Palermo, nel corso dell’ultimo anno, sono stati isolati stipiti di D. capitatus. I ceppi, provenienti da tre pazienti, da campioni diversi e da differenti reparti, sono stati identificati fenotipicamente, utilizzando il CHROMagar Candida (BD), e la loro identificazione è stata confermata tramite sequenziamento. Inoltre, al fine di valutare l’eventuale diffusione epidemica di questi isolati, sono stati utilizzati la PCR fingerprinting che prevede l’utilizzo della sequenza core del fago M13 e la RAPD (Random Amplification of Polymorphic DNA) utilizzando il primer Ope-4 e la combinazione dei primer W80A-Ap12h. more...
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- 2016
48. Colonization of pressure ulcers by multidrug-resistant microorganisms in patients receiving home care
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Caterina Mammina, Anna Rita Mattaliano, Aurora Aleo, Celestino Bonura, Maria Concetta Cataldo, Teresa Fasciana, Giovanna Rizzo, Giuseppe Caputo, Cinzia Calà, Daniela Maria Geraci, Cataldo, MC, Bonura, C, Caputo, G, Aleo, A, Rizzo, G, Geraci, DM, Calá, C, Fasciana, T, Mattaliano, AR, and Mammina, C more...
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Male ,Methicillin-Resistant Staphylococcus aureus ,Microbiology (medical) ,medicine.medical_specialty ,multidrug-resistant ,Drug resistance ,medicine.disease_cause ,Drug Resistance, Multiple, Bacterial ,Internal medicine ,Gram-Negative Bacteria ,Epidemiology ,Humans ,Medicine ,In patient ,Colonization ,Intensive care medicine ,Aged ,Aged, 80 and over ,Pressure Ulcer ,General Immunology and Microbiology ,biology ,business.industry ,Skin Diseases, Bacterial ,General Medicine ,Middle Aged ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,biology.organism_classification ,Antimicrobial ,Home Care Services ,Anti-Bacterial Agents ,Multiple drug resistance ,Infectious Diseases ,Italy ,Enterococcus ,Staphylococcus aureus ,Female ,home care ,business - Abstract
Colonization and/or infection with multidrug-resistant microorganisms (MDRO) of pressure ulcers in patients receiving care at home have seldom been investigated. The objective of this study was to assess the prevalence of MDRO colonization in pressure ulcers of patients receiving home care in Palermo, Italy. Vancomycin-resistant Enterococcus (VRE), methicillinresistant Staphylococcus aureus (MRSA), and multidrug-resistant Gram-negative bacilli (MDRGN) were isolated, identifi ed, and characterized from pressure ulcers and selected home environment surfaces. Thirty-two patients were enrolled, of whom 12 were under antimicrobial therapy. Five patients had been admitted to hospital in the preceding year. Nineteen patients tested positive for 1 or more MDROs. In particular, 1 patient was colonized by a vanA-containing strain of VRE, 5 by MRSA, and 17 by MDRGN of different species. Our fi ndings suggest that pressure ulcers in home care patients could play a role in bringing MDROs into the community setting. more...
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- 2011
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49. Rapid Identification by MALDITOF of Neisseria elongata Subspecies nitroreducens in an Endocarditis Case
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C. Bonura, A. Giammanco, Indelicato S, G Giordano, Novo S, Aquilina G, Cinzia Calà, Di Gaudio F, T. Fasciana, Parrinello R, Fasciana, T, Di Gaudio, F2, Novo, S, Aquilina, G, Indelicato, S, Giordano, G, Parrinello, R, Bonura, C, Calà, C, and Giammanco, A more...
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biology ,business.industry ,Subspecies ,Neisseria elongata ,biology.organism_classification ,medicine.disease ,Microbiology ,Rapid identification ,Infective endocarditis ,medicine ,Endocarditis ,infective endocarditis ,business ,Pathogen - Abstract
Background: Neisseria elongata subspecies nitroreducens is considered to be an important pathogen responsible for infective endocarditis, even if it is infrequently reported. We report the first case of endocarditis in Italy due to N. elongata subsp. nitroreducens. Case presentation: The infection occurs in a 40-year-old male affected by Marfan syndrome with a prosthetic aortic valve. The patient had had a fever for a week which had been resistant to antibiotic therapy. Conclusion: we propose a correct and rapid identification of Neisseria elongata subspecies nitroreducens by mass spectrometry directly from the positive blood culture. The rapidly identification obtained by MALDI-TOF it has enabled us to administer a correct empirical therapy. more...
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- 2016
50. Fourth case of louse-borne relapsing fever in Young Migrant, Sicily, Italy, December 2015. Mini Review Article
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Antonio Cascio, Marcello Trizzino, Tma Fasciana, P. Di Carlo, Anna Giammanco, Claudia Colomba, Francesco Scarlata, Colomba, C., Scarlata, F., Di Carlo, P., Giammanco, A., Fasciana, T., Trizzino, M., and Cascio, A. more...
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myalgia ,Adult ,Male ,medicine.medical_specialty ,Pediatrics ,Refugee ,relapsing fever ,Somalia ,030231 tropical medicine ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Epidemiology ,Borrelia recurrentis ,Europe ,Louse-borne relapsing fever ,Refugees ,Public Health, Environmental and Occupational Health ,medicine ,Humans ,030212 general & internal medicine ,Louse-Borne Relapsing Fever ,Sicily ,Transients and Migrants ,biology ,Borrelia recurrenti ,business.industry ,Public health ,Borrelia ,Relapsing Fever ,General Medicine ,Lice Infestations ,medicine.disease ,biology.organism_classification ,Family medicine ,Chills ,medicine.symptom ,Headaches ,business - Abstract
Objectives Currently louse-borne relapsing fever (LBRF) is primarily found in limited endemic foci in Ethiopia, Somalia and Sudan; no case of imported LBRF has been reported in Europe in the 9 years prior to 2015. The aim of our paper is to describe a new case of imported LBRF detected in Sicily, Italy, and to review all cases reported in migrants arrived in Europe in the last 10 years. Study design Mini review of all published cases of louse-borne relapsing fever in Europe in the last 10 years. Methods A computerized search without language restriction was conducted using PubMed combining the terms ‘(louse-borne relapsing fever or LBRF or recurrentis) and (refugee or Europe or migrant)’ without limits. Furthermore, the ‘Ahead-of-Print Articles’ of the top 10 journals (ranked by Impact factor – Web of Science) of Infectious diseases and of Epidemiology were checked. Results Our search identified 26 cases of LBRF between July and October 2015 in migrants recently arrived in Europe: 8 had been described in Italy; 1 in Switzerland; 2 in the Netherlands; 15 in Germany. We describe data regarding the clinical characteristics, diagnostic methods, therapy and outcome of these patients and of the new case. Conclusions LBRF by Borrelia recurrentis should be considered among the clinical hypotheses in migrants presenting with fever, headache, chills, sweating, arthralgia, myalgia, dizziness, nausea and vomiting. more...
- Published
- 2016
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