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Maize (Zea mays L.) is the foremost cereal consumed in Benin but its production is constrained by the lack of access to high-quality seeds for intensification of the crop; hence, the continued cultivation of disease-susceptible, low-yielding, and open-pollinated varieties (OPVs). The study (i) evaluated the agronomic performance and heterosis of topcross hybrids (THs) developed from a cross between new streak-resistant inbred lines and Benin-adapted OPVs, (ii) estimated the combining ability of the parental materials for grain yield (GY), and (iii) assessed the relationship between GY and other agronomic traits. Eleven OPVs were crossed to two inbred lines in a line × tester mating design to generate 22 THs. The THs were evaluated across four environments in Benin. The environment had a significant effect on GY and agronomic traits. General and specific combining ability effects were significant for GY and other traits indicating the presence of additive and non-additive gene effects. Four THs exhibited positive mid- and better-parent heterosis for GY and DMR ESR W × TZIL07A01322, which is the highest yielding TH, at 5.1 tons per hectare, had 29.1 and 13.3% yield advantages over its mid-parent and better-parent mean yields, respectively, with the potential for commercialization by indigenous seed enterprises. GY was positively associated with 100-seed weight enabling indirect selection. Four OPVs contributed favorably to observed heterosis and could serve as potential genotypes for inbred line extraction.

Abstract The present study aimed to assess mosquito species diversity, distribution, and ecological preferences in the Covè, Ouinhi, and Zangnanado communes, Southern Benin. Such information is critical to understand mosquito bio-ecology and to focus control efforts in high-risk areas for vector-borne diseases. Mosquito collections occurred quarterly in 60 clusters between June 2020 and April 2021, using human landing catches. In addition to the seasonal mosquito abundance, Shannon's diversity, Simpson, and Pielou's equitability indices were also evaluated to assess mosquito diversity. Ecological niche models were developed with MaxEnt using environmental variables to assess species distribution. Overall, mosquito density was higher in the wet season than in the dry season in all communes. A significantly higher Shannon's diversity index was also observed in the wet season than in the dry seasons in all communes (p

This study aims to assess the level of sustainability in vegetable-based agrifood production systems in Benin and to propose actions to enhance sustainability, food safety, and year-round production in the vegetable production systems. Semi-structured interviews were conducted with 200 vegetable farmers in contrasting agroecological areas (with areas of extensive production of staples and intensive production of vegetables), using the “Indicateur de Durabilité des Exploitations Agricoles” (IDEA) framework (an on-farm sustainability index). Most of the surveyed vegetable farmers produced a wide range of crops, including leafy vegetables (amaranth, African eggplant, and African basil) and peppers, grown by more than 50% of the farmers. The average scores achieved by the vegetable farms regarding three dimensions of sustainability—ecological, social, and economic—were 35, 41, and 63, respectively, out of a maximum score of 100. All three sustainability dimensions of the vegetable farms were, on average, at a low level and improvements were needed for them to reach an acceptable standard. The vegetable farms located in the south of Benin had, on average, a higher sustainability score than those in the north: around 50% of vegetable farms in the south had a medium score, while the sustainability level of almost 75% of vegetable farms in the north was low. Interventions seeking to improve the sustainability of vegetable farms in Benin should focus on the promotion and adoption of eco-responsible practices that improve on-farm biodiversity, water conservation, and the effective allocation and management of land and labor, to mitigate the environmental impacts of vegetable production.

Although agronomic biofortification has been argued as strategic approach to cope with food and nutrition insecurity in many areas, smallholders’ farmers in sub-Saharan Africa (SSA) are still facing challenges to produce cereals and legumes rich in micro nutrients such as iron, zinc, calcium and magnesium. This paper review progress made in agronomic biofortification in cereals (maize and rice) and legumes in iron, zinc, calcium and magnesium, and discussed perspective for further researches and uses of agronomic biofortification in maize, rice and legume crops in sub-Saharan Africa. The review clearly indicated that agronomic biofortification offers many options for cereals (maize and rice) and legumes (common bean, chickpea, lentil, mung bean, cowpea, etc.) based on chemical fertilizers (ZnSO4, FeSO4, Fe-chelates, MgSO4, MgCl2, urea, NPK) and traditional nutrient sources (bioinoculants, farm yard manure, compost, cereal-legume intercropping system), nutrient application methods (seed priming, soil and foliar application), nutrient application rate and period of application that are crop dependent. Bottlenecks to its wide adoption by SSA farmers are among others the low availability of micronutrient-based fertilizers, low awareness, and low financial capacity. Current trends in SSA agriculture support agroecological farming based on the use of local sources of micronutrients and strategies such as compost tea, organic manure, intensive production system, coated fertilizers with plant growth promoting bacteria, plant residue incorporation, cereal-legumes intercropping. Further research should focus on the effectiveness of different agroecological strategies/sources of micronutrients along with their content in micronutrients, the availability/translocation of these micronutrients in the plants, the application time and quality of harvested products.

This review provides evidence on the genetic diversity, chemical constituents, and ecotoxicology of Mesosphaerum suaveolens ‘ essential oil. It emphasizes the agricultural benefits such as crop protection effectiveness of the plant and highlights the existing knowledge gaps and research perspectives to promote its utilization in agriculture. A systematic and extensive review of the literature was done and all pertinent full-text articles and abstracts were analyzed and incorporated into the review. Mesosphaerum suaveolens is used traditionally in pharmacology to treat several diseases such as malaria, constipation, stomach problems, and renal inflammation. It also treats cramps, digestive infections, headaches, and skin infections. To date, very few studies have been conducted worldwide about its genetic diversity. These studies highlighted three morphological variants, the blue-flowering, the white-flowering, and the light-purple flowering M. suaveolens. Its wide biological actions may be attributed to the numerous groups of chemical constituents in its essential oil including monoterpenes, sesquiterpenes, and diterpenes. Biological studies highlighted evidence of M. suaveolens being used as an antifungal, bactericidal, antimicrobial, insecticidal, and repellent plant. The essential oil extracted from M. suaveolens showed significant potential for the control of agricultural pests such as Sitiophilus zeamais, Helicoverpa armigera and Helminthosporium oryzae. M. suaveolens is commonly used worldwide as a pesticidal plant in healthcare, agriculture, and food preservation. However, there is a lack of studies concerning the toxicity and effectiveness of isolated potent phytotoxic substances, the efficacy screening in the field, the genetic diversity, the essential oil yield, and productivity. Consequently, further studies are required to fill the knowledge gaps.

Le climat et la fauche influencent la production fourragère au Sahel. Cependant, l’effet combiné de ces paramètres reste à évaluer. Cette étude vise à évaluer l’effet conjoint de la pluviométrie et des pratiques de fauche sur la quantité et la qualité des fourrages. Des échantillons de phytomasse ont été prélevés au cours et à la fin de la saison des pluies 2021 par coupe intégrale sur 68 parcelles : 20 parcelles avec différents régimes hydriques et 48 parcelles avec différentes quantités d’eau combinées à différentes hauteurs et périodes de fauche. Les valeurs de la phytomasse sèche étaient respectivement comprise entre 2 932,2 ± 1 672,1 et 6 383,6 ± 2 962,6 kg/ha, 2 397,7 ± 6 263,4 et 15 059,2 ± 9 782,9 kg/ha pour les traitements de régime hydrique et du cumul de phytomasse fauchée. Le taux de cellulose brute (en % de Matière sèche) était compris entre 5,5 ± 0,9 % et 6,4 ± 1,2 %. La matière azotée digestible a varié entre 21,8 ± 67,96 et 67,2 ± 15,8 %. Quelle que soit la quantité d’eau reçue, des quantités et qualités équivalentes de fourrages ont été produites par les parcelles non fauchées et celles fauchées au début du cycle de développement des espèces. Pour une fauche à 0 ou 5 cm au-dessus du sol, la quantité de phytomasse était la même. Les parcelles fauchées au stade de fructification des espèces ont produit des fourrages composés de jeunes plantes en quantités plus faibles et de bonne qualité. La quantité et la qualité de fourrage herbacé en fin de saison des pluies ont surtout été influencées par le stade phénologique. Les programmes de gestion des ressources fourragères devront privilégier des périodes et hauteurs de coupe permettant une couverture optimale du sol afin de réduire les risques d’érosion.

Abstract Background Indoor residual spraying (IRS) was first implemented in the Atacora department, Benin from 2011 to 2012 using bendiocarb (carbamate) followed by annual spraying with pirimiphos-methyl (organophosphate) from 2013 to 2018. Before and after IRS implementation in Atacora, standard pyrethroid insecticide-treated bed nets were the main method of vector control in the area. This study investigated the knockdown resistance (kdr) gene (L1014F) and the acetylcholinesterase (ace-1) gene (G119S), before and during IRS implementation, and 4-years after IRS withdrawal from Atacora. This was done to assess how changes in insecticide pressure from indoor residual spraying may have altered the genotypic resistance profile of Anopheles gambiae s.l. Method Identification of sibling species of An. gambiae s.l. and detection of the L1014F mutation in the kdr gene and G119S mutation in ace-1 genes was done using molecular analysis. Allelic and genotypic frequencies were calculated and compared with each other before and during IRS implementation and 4 years after IRS withdrawal. The Hardy–Weinberg equilibrium and genetic differentiation within and between populations were assessed. Results Prevalence of the L1014F mutation in all geographic An. gambiae s.l. (An. gambiae s.s., Anopheles. coluzzii, Anopheles. arabiensis, and hybrids of “An. gambiae s.s. and An. coluzzii”) populations increased from 69% before IRS to 87% and 90% during and after IRS. The G119S allele frequency during IRS (20%) was significantly higher than before IRS implementation (2%). Four years after IRS withdrawal, allele frequencies returned to similar levels as before IRS (3%). Four years after IRS withdrawal, the populations showed excess heterozygosity at the ace-1 gene and deficit heterozygosity at the kdr gene, whereas both genes had excess heterozygosity before and during IRS (F IS

Abstract Background To assess the adherence to option B + antiretroviral therapy (ART) and associated factors in pregnant and breastfeeding women in Sub-Saharan Africa (SSA). Methods We conducted a comprehensive search from 01st January 2012 to 03rd October 2022, across four databases: PubMed, Scopus, Proquest Central, and Index Medicus Africain, to identify studies focused on pregnant and/or breastfeeding women living with HIV and receiving option B+ ART in SSA. Studies reporting adherence data were included in the meta-analysis. Were excluded studies published before 01st January 2012, grey literature, systematic reviews, and meta-analysis studies. Articles selection and data extraction were performed independently by two reviewers. We evaluated pooled adherence and pooled association between various factors and adherence using a random-effects model. Results Overall, 42 studies involving 15,158 participants across 15 countries contributed to the meta-analysis. The overall pooled adherence was 72.3% (95% CI: 68.2–76.1%). Having high education level (pooled odds ratio (OR): 2.25; 95% CI: 1.57–3.21), living in urban area (pooled OR: 1.75; 95% CI: 1.10–2.81), disclosing status to a family/partner (pooled OR: 1.74; 95% CI: 1.27–2.40), having a support system (pooled OR: 3.19; 95% CI: 1.89–5.36), receiving counseling (pooled OR: 3.97; 95% CI: 2.96–5.34), initiating ART at early clinical HIV stage (pooled OR: 2.22; 95% CI: 1.08–4.56), and having good knowledge on PMTCT/HIV (pooled OR: 2.71; 95% CI: 1.40–5.25) were factors significantly associated with adherence to option B + ART. Conclusions Despite the implementation of option B+ ART, the level of adherence among pregnant and breastfeeding women in SSA falls short of meeting the critical thresholds for viral load suppression as outlined in the 95-95-95 objectives set for 2025. These objectives are integral for achieving HIV elimination, and in turn, preventing HIV mother-to-child transmission. To bridge this gap, urgent tailored interventions based on individual and structural factors are essential to enhance adherence within these subgroups of women. This targeted approach is crucial in striving towards the HIV elimination target in SSA.

The Great Green Wall (GGW) is an international initiative to combat land degradation and restore native plant life in the Sahel, but due to a lack of monitoring tools, it remains unknown to be considered as success or failure. Here, we quantify the impact of GGW plantations and Corporate plantations (privately owned) in Sahelian Senegal based on remote sensing data and deep learning by mapping individual trees and their biomass across the Sahel region. Tree features (cover, density and above-ground biomass) have been computed in every hectare of 42 plantations (of both, corporate and GGW) and their surrounding non-planted areas, subsequently used for a comparative analysis of tree features. Results showed that gains in tree features varied substantially between plantations. At plot scale, among Corporate plantations, the average gain in tree density was 61.16±42.12 trees/ha while it was 5.7 ± 5.8 trees/ha for GGW plantations. In regards to tree cover, the average gain was 618.5 ± 588.5 m2/ha for Corporate plantations and 71.72±108.89 m2/ha for GGW plantations. For the above-ground biomass, the average gain was 3.36±3.29 tons/ha in the Corporate plantations and 0.46±0.67 tons/ha in the GGW plantations. The average gain in foliar biomass in the Corporate plantations was 0.15±0.13 tons/ha and in the GGW plantations, it was 0.02±0.03 tons/ha. The average gain in wood biomass was 3.21±3.12 tons/ha among the Corporate plantations and was 0.43±0.64 tons/ha among the GGW plantations. Notably, regarding the relative benefit in terms of ecosystem services per unit of density, each tree in GGW plantations contribute more to ecosystem services per unit of density compared to Corporate plantations. In GGW, each gained tree contributes 18 m2 of cover, 116.1 kg of above-ground biomass, 5.6 kg of foliar biomass, and 114.2 kg of woody biomass, while in corporate plantations, on the other hand, each gained tree adds 9 m2 of cover, 48.4 kg of above-ground biomass, 2.3 kg of foliar biomass, and 46.3 kg of woody biomass. These findings are opposed to conventional paradigms, suggesting that the Great Green Wall, while perhaps missing its tree density targets, has succeeded in its mission to produce ecosystem services per tree. This raises important questions about the redefinition of objectives in reforestation projects, focusing on quality rather than quantity, a perspective that could transform our understanding of the successes and failures of these essential ecological restoration initiatives. However, the assessment primarily relies on indicators such as cover and biomass, potentially overlooking other crucial ecosystem services. Therefore, while our conclusions underscore the effectiveness of reforestation initiatives, future research should aim to establish a more comprehensive understanding of ecosystem services by incorporating additional indicators beyond cover and biomass, such as species diversity, soil health, water retention, and habitat quality.

Abstract Background The savannah ecosystems of Sahel have experienced continuous and heavy grazing of livestock for centuries but still, their vegetation response to grazing pressure remains poorly understood. In this study, we analysed the herbaceous plant dynamics, measured by species diversity, composition, cover, and biomass in response to grazing pressure in the savannah ecosystems of Sahel. In Senegal, we selected four savannah sites represented with high, moderate, light and no grazing intensity levels. Transect survey methods were used for sampling the vegetation data within each of the sites. Species richness and composition were analysed using species accumulation curve and multivariate analyses. Furthermore, we used General Linear Models and a piecewise Structural Equation Model (pSEM) to examine the relationships between grazing intensity, vegetation cover, diversity and biomass. Results The herbaceous species diversity and composition varied significantly among the different grazing intensity levels (p

Orange-fleshed sweetpotato (OFSP) is a sustainable and inexpensive source of vitamin A that contributes to food and nutritional security in developing countries. Its adoption is low due to unconformity of the variety with community food culture, limited cultivar selection and breeding studies. The current study aimed at breeding of promising OFSP genotypes in Benin using three parental lines. Two hundred and seventy-four seeds and one hundred and six genotypes were obtained through biparental crosses of Tio-joe (imported orange-fleshed variety), Vobodouaho (local white-fleshed variety) and ACAB220 (local orange-fleshed variety). The phenotypic diversity of genotypes was assessed using 10 leaf characters and storage root flesh colour through multiple correspondence analysis and hierarchical cluster analysis. The results indicated cross-compatibility between Tio-joe and ACAB220 when ACAB220 was used as female. Reciprocal cross-compatibility was found between ACAB220 and Vobodouaho. The genotypes could be separated into two main populations and classified into five cluster groups. Promising intermediate and pale orange genotypes were obtained indicating possibilities to incorporate beta-carotene into the white background of the Vobodouaho variety through further backcrossing.

Abstract Selection of mosquito collection methods is of crucial importance to evaluate the impact of vector control tools on entomological outcomes. During a cluster randomised control trial evaluating the relative efficacy of two dual-active ingredient (a.i.) long-lasting insecticidal nets (LLINs) compared to pyrethroid-only LLINs, we assessed the performance of different mosquito collection methods: Human landing catches (HLC), Centers for Disease Control and Prevention (CDC) light traps, and pyrethrum spray catches (PSC). Anopheles mosquitoes were collected using three collection methods in 4 houses, in each of the 60 trial clusters at baseline and every quarter for 24 months using PSCs and HLCs, while CDC light traps were performed during two quarters only. Mean density of vectors collected per method per night was the highest with HLCs (15.9), followed by CDC light traps (6.8); with PSCs (1.1) collecting 10 times less mosquitoes than HLCs. All three collection methods collected fewer mosquitoes in the Interceptor G2® dual a.i. arm, compared to the other trial arms, although only HLCs and PSCs demonstrated strong evidence of this due to a greater number of collection rounds undertaken, than CDC light traps. The broadly similar results regarding the differential impact of the two dual a.i. LLINs showed by the three collection methods suggest that the more ethically acceptable, cheaper, and logistically simpler methods such as CDC light traps could be prioritised for use in large community trials for measuring the efficacy of vector control tools.

Abstract Background Provider-Initiated HIV Testing and Counseling (PITC) and Prevention of Mother-To-Child Transmission (PMTCT) are key services for achieving the goal of complete elimination of HIV. However, there is limited evidence on the ability of health facilities to provide these services in Burkina Faso. Therefore, we aimed to assess the trends and disparities in the availability and readiness of health facilities to provide PITC and PMTCT services in Burkina Faso between 2012 and 2018. Methods We performed a secondary analysis of facility-level data from the World Health Organization’s Service Availability and Readiness Assessment (SARA) surveys conducted in 2012, 2014, 2016, and 2018 in Burkina Faso. The availability and readiness of health facilities were assessed using SARA’s manual, and linear regressions were used to examine trends. Results Between 2012 and 2018, the mean proportion of health facilities providing PITC services increased, but not significantly, from 82.9% to 83.4% (p = 0.11), with the mean readiness index significantly decreasing from 71.5% to 65.4% (p

In 2022, an estimated 1.25 million children 50% remained undiagnosed or unreported. WHO recently recommended integrated and decentralized models of care as an approach to improve access to TB services for children, but evidence remains limited. The Catalyzing Paediatric TB Innovation project (CaP-TB) implemented a multi-pronged intervention to improve TB case finding in children in nine sub-Saharan African countries. The intervention introduced systematic TB screening in different facility-based child-health entry-points, decentralisation of TB diagnosis and management, improved sample collection with access to Xpert® MTB/RIF or MTB/RIF Ultra testing, and implementation of contact investigation. Pre-intervention records were compared with those during intervention to assess effect on paediatric TB cascade of care. The intervention screened 1 991 401 children

Abstract Objective To assess the 18-month field effectiveness on severe events of a pediatric package combining early HIV-diagnosis and targeted cotrimoxazole prophylaxis in HIV-infected children from age six-week before the antiretroviral era, in Abidjan, Côte d'Ivoire. Methods Data from two consecutive prevention of HIV mother-to-child transmission programs were compared: the ANRS 1201/1202 Ditrame-Plus cohort (2001–2005) and the pooled data of the ANRS 049a Ditrame randomized trial and its following open-labeled cohort (1995–2000), used as a reference group. HIV-infected pregnant women ≥ 32–36 weeks of gestation were offered a short-course peri-partum antiretroviral prophylaxis (ZDV in Ditrame, and ZDV ± 3TC+single-dose (sd) NVP in Ditrame-Plus). Neonatal prophylaxis was provided in Ditrame-Plus only: 7-day ZDV and sdNVP 48–72 h after birth. A 6-week pediatric HIV-RNA diagnosis was provided on-line in the Ditrame-Plus while it was only oriented on clinical symptoms in Ditrame. Six-week HIV-infected children received a daily cotrimoxazole prophylaxis in Ditrame-Plus while no prophylaxis was provided in Ditrame. The determinants of severe events (death or hospitalization > 1 day) were assessed in a Cox regression model. Results Between 1995 and 2003, 98 out of the 1121 live-births were diagnosed as HIV-infected in peri-partum: 45 from Ditrame-Plus and 53 from Ditrame. The 18-month Kaplan-Meier cumulative probability of presenting a severe event was 66% in Ditrame-Plus (95% confidence interval [95%CI]: 50%–81%) and 77% in Ditrame (95%CI: 65%–89%), Log Rank test: p = 0.47. After adjustment on maternal WHO clinical stage, maternal death, 6-week pediatric viral load, birth-weight, and breastfeeding exposure, the 18-month risk of severe event was lower in Ditrame-Plus than in Ditrame (adjusted Hazard Ratio (aHR): 0.55, 95%CI: 0.3–1.1), although the difference was not statistically significant; p = 0.07). Maternal death was the only variable determinant of the occurrence of severe events in children (aHR: 3.73; CI: 2.2–11.2; p = 0.01). Conclusion Early cotrimoxazole from 6 weeks of age in HIV-infected infants seemed to reduce probability of severe events but the study lacked statistical power to prove this. Even with systematic cotrimoxazole prophylaxis, infant morbidity and mortality remained high pointing towards a need for early pediatric HIV-diagnosis and antiretroviral treatment in Africa.

Increasing production requires the development of high yielding cultivars adapted to various environments. Multi-environment trials (MET) remains the best approach to assess the performance of accessions across environments. The objective of this study was to select the best performing and stable accessions of sesame across different environments in Benin Republic. Nineteen sesame accessions were evaluated across eight environments using a randomized complete block design with four replicates. The accessions were evaluated for three traits: days to 50% flowering (D50F), thousand-seed weight (TSW), and seed yield (SY) during 2020 growing season. The stable and top-performing accessions across environments were determined using AMMI (Additive main effects and multiplicative interaction), GGE (Genotype main effect and genotype × environment interaction), and MTSI (Multi-trait stability index). AMMI analysis of variance showed a significant difference across environments for the three traits. The accessions were affected by environmental conditions for the three traits. The broad-sense heritability estimates were high (>0.60) for all the traits, indicating the improvement is achievable through selection. AMMI1 and AMMI2 biplots identified G10 and G13 as high seed yielding accessions adapted to environments E1, E2. The GGE biplot showed two mega-environments for TSW and three mega-environments for D50F and SY. For SY, G11 and G13 were the best accessions in the first mega-environment, G10 the best accession in the second mega-environment; G3 and G8 were the best accessions in the third mega-environment. AMMI and GGE analyses identified G10, G5, G12 as high seed yielding and stable accessions across environments. GGE biplot revealed that E1 and E2 were the most suitable environments for multi-location trials based on their discriminating ability and representativeness. MTSI indicated G10, G13, G19 as promising germplasm to be recommended for breeding program.

Abstract Background The use of services to prevent mother-to-child transmission (PMTCT) of the human immunodeficiency virus (HIV) remains a serious challenge in sub-Saharan Africa. In the last decade, Burkina Faso has implemented numerous policies to increase the use of PMTCT services by pregnant women and their partners, as well as children. This study assesses trends in the uptake of PMTCT services in Burkina Faso from 2013 to 2020 in order to study the progress and gaps in achieving the national and international targets set for 2020. Methods A repeated cross-sectional analysis was performed using data extracted from district health information software version 2. Percentages were computed for each PMTCT indicator and comparisons between the years were made using a chi-square test for trends with a significance threshold of 5%. Regions were not compared with each other. Results The proportion of pregnant women who were tested and received their results significantly increased from 47.9% in 2013 to 84.6% in 2020 (p value

Abstract Cochlospermum spp. root powder is widely used by local populations in Sudanian zone of Benin as food ingredient. This study aims at documenting the mineral profile of Cochlospermum spp. root powder and the consumption frequencies of foods enriched with this species in the three phytodistricts of Sudanian zone of Benin. A consumption frequency survey was conducted among 449 women of reproductive age and their children (n = 406) aged 6 to 59 months. Processing follow‐up was performed among twelve (12) selected processors per phytodistict and samples were collected for minerals (Ca, Mg, Na, P, Fe, Zn, Cu, and Mn) analysis. Results showed that most (>75%) women and their children consumed foods enriched with Cochlospermum spp. root powder at least two times per day. The mineral contents (expect Ca and P) of Cochlospermum spp. root powder do not vary significantly according to phytodistricts. High mineral content was obtained for iron (54.1 ± 21.5 mg/100 g dry weight) and calcium (573.7 ± 98 and 802.5 ± 49.9 mg/100 g dry weight, respectively in Atacora Chain and Northern Borgou phytodistricts). Cochlospermum spp. root powder could be used to alleviate iron and calcium deficiencies among populations. Further studies should focus on the bioavailability of minerals and anti‐nutrients content of this plant food.

IntroductionQuality seed is essential to satisfy food demand. This is also true for neglected crops especially those that are economically valuable such as Kersting’s groundnut (Macrotyloma geocarpum), which holds the potential to improve farmers’ livelihoods. In this study, we assessed the attributes that drove Kersting groundnut farmers’ willingness to pay for improved seeds.MethodsA total of 567 respondents were selected in the Northern Guinea and Southern Sudanian production zones in Benin and Togo using chain referral sampling, and they were then interviewed with a semi-structured questionnaire. Classification and regression trees, Ordinary Least Squared, and Tobit regression were combined to assess the relationship between the socio-demographic variables and farmers’ Willingness to Pay (WTP) and Amount they are Willing to Pay (AWTP).Results and discussionResults suggested that more than 90% of respondents involved in the production of Macrotyloma geocarpum were willing to access its improved seeds, including those who had already abandoned the cultivation of this crop due to constraints such as the very low seed yield of current cultivars, the difficulty to access seeds, and the cultivation practices. The factors which affected the amount farmers are willing to pay included the following: the low yield of current cultivars used by farmers, the expected yield of the improved variety, which should be higher than 1 ton per ha (1 t.ha−1), the socio-linguistic group affiliation, and the adoption level of improved agricultural technologies. The average amount fixed by seed companies that farmers were willing to pay for 1 kg of the improved seed was USD 5.35 but they have freely proposed to pay the average amount of USD 4.63 to access 1 kg of improved seed. The white-seeded cultivar was the most appreciated by farmers and was the most cultivated in the Northern Guinean Zone whereas the cultivation of the colored-seeded (e.g., black-seeded) cultivars was mainly noted in the Southern Sudanian Zone. Furthermore, the respondents indicated seed yield improvement and disease management as their main research needs to help increase the production of the crop. The findings of this research will help refine Macrotyloma geocarpum improvement programs to release farmer-needed varieties.

Wedminere Noëlie Zoungrana-Yameogo,1 Lucresse Corine Fassinou,2 Calypse Ngwasiri,3 Sekou Samadoulougou,4,5 Isidore Tiandiogo Traoré,1,6 Hervé Hien,1,6 Fidèle Bakiono,7 Maxime Drabo,8,9 Fati Kirakoya-Samadoulougou3 1Service d’information et d’épidémiologie, Centre Hospitalier Universitaire de Tengandogo, Ouagadougou, Burkina Faso; 2Institut Supérieur des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, Burkina Faso; 3Centre de Recherche en Epidémiologie, Biostatistique et Recherche Clinique, Ecole de Santé Publique, Université Libre de Bruxelles, Bruxelles, Belgique; 4Evaluation Platform on Obesity Prevention, Quebec Heart and Lung Institute, Quebec, QC, G1V 4G5, Canada; 5Centre for Research on Planning and Development (CRAD), Université Laval, Quebec, QC, G1V 0A6, Canada; 6Centre Muraz, Institut National de Santé Publique, Bobo-Dioulasso, Burkina Faso; 7Secrétariat Permanent du conseil national de lutte contre le SIDA et les infections sexuellement transmissible, ministère de la santé, Ouagadougou, Burkina Faso; 8Département biomédical et santé publique, Institut de recherche en sciences de la santé, Ouagadougou, Burkina Faso; 9Laboratoire de santé publique de l’Ecole doctorale des sciences de la santé (ED2S), Université Joseph Ki Zerbo Ouagadougou, Ouagadougou, Burkina FasoCorrespondence: Lucresse Corine Fassinou, Institut Supérieur des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, Burkina Faso, Email corinefas123@gmail.comBackground: Since the scale-up of antiretroviral therapy (ART) services in Burkina-Faso, achieving an AIDS-free generation depends on optimal ART adherence. However, no data exists on the population group differences on the level of ART adherence in Burkina-Faso. This study analyzes ART adherence among pregnant-and breastfeeding-women, non-pregnant women, and men in Burkina-Faso.Methods: From December 2019 to March 2020, a cross-sectional study among adult HIV-infected patients in Burkina-Faso, belonging either to the active file or the Prevention of Mother-To-Child Transmission programs was conducted. An analysis was performed and adherence was measured based on the number of times, patients did not meet the number of doses prescribed and did not take the ART treatment in the month prior to the survey. Logistic-regression models were used to identify factors associated with poor ART adherence and the adjusted odds ratios (aORs) with their 95% confidence intervals (95% CI) were reported.Results: The prevalence of good adherence was higher in the group of pregnant-and breastfeeding-women (86.6%, n = 112) compared to the other groups (73.1%, n=1017 in non-pregnant women and 72.0%, n = 318 in men). No association was found between ART adherence and the socio-demographic, clinical, and therapeutic characteristics of pregnant-and breastfeeding-women and men groups. However, non-pregnant women with a high level of education (aOR = 1.70; 95% CI: 1.16– 2.49), having ever belonged to a support group (aOR= 1.47; 95% CI: 1.07– 2.04), not having income-generating occupations (aOR= 1.53; 95% CI: 1.11– 2.12), and in advanced clinical stage (aOR= 1.42; 95% CI: 1.06– 1.91) were more susceptible to have poor adherence compared to their pairs.Conclusion: Findings showed a large difference in ART adherence among pregnant-and breastfeeding-women, non-pregnant women and men and highlight the need for differentiated healthcare delivery according to population while specifically considering addressing the interest in early initiation of treatment and the benefit of support groups meeting.Keywords: adherence, HIV, antiretroviral therapy, PMTCT, Burkina Faso

Combretum racemosum, a plant from the Combretaceae family, is traditionally used in Benin for various health problems. However, scientific research on Beninese samples of this plant is limited. The aim of this study was to identify and assess the bioactive compounds in the plant’s leaves and roots. Initial screening involved analyzing powders derived from these parts for total polyphenols, flavonoids, and both condensed and hydrolyzable tannins. The polyphenolic compounds were analyzed using HPLC-DAD-ESI-MS. To evaluate the plant’s antimicrobial properties, the agar diffusion method was employed, while FRAP and DPPH assays were used to determine its antioxidant capacity. For anti-inflammatory activity, the study utilized tests for in vitro protein denaturation inhibition and in vivo acute edema induced by carrageenan. Additionally, an antiproliferative assay was conducted using the human melanoma cell line A375. The analysis revealed the presence of significant polyphenolic compounds in both the leaf and root extracts of C. racemosum. Notably, compounds like Pedunculagin, Vescalagin, Casuarictin, and Digalloyl-glucoside were abundant in the leaves, with Vescalagin being especially predominant in the roots. The study also found that the dichloromethane extracts from the leaves and roots exhibited bactericidal effects on a substantial percentage of meat-isolated strains. Moreover, the antioxidant activities of these extracts were confirmed through FRAP and DPPH methods. Interestingly, the dichloromethane root extract showed strong activity in inhibiting thermal albumin denaturation, while the water–ethanol leaf extract demonstrated significant edema inhibition. Finally, the study observed that C. racemosum extracts reduced cell viability in a dose-dependent manner, with leaf extracts showing more pronounced antiproliferative effects than root extracts. These findings highlight the potential of C. racemosum leaves and roots as sources of compounds with diverse and significant biological activities. In conclusion, C. racemosum’s leaves and roots exhibit promising biological activities, highlighting their potential medicinal value.

The inventory of the carbon (C) pools in Africa’s ecosystems is not well documented, although it is crucial to support climate mitigation policies. We quantified the C stocks in plant biomass, woody necromass, litter and soil (0–30 and 30–100 cm) for the five main land uses – forest, tree plantation, young and adult palm groves, croplands – of Ferralsols on the Allada plateau in southeast Benin. Forests have the highest total C stocks (389 ± 54 Mg C ha−1) compared with other land uses (222 ± 33, 154 ± 6, 105 ± 2, 77 ± 3 Mg C ha−1 in tree plantations, adult palm groves, young palm groves and croplands, respectively). The C stocks are higher in the biomass than in the soil (0–100 cm), e.g. in the forest, stocks were 279 ± 54 Mg C ha−1 in the biomass versus 83 ± 2 Mg C ha−1 in the soil. Differences of soil C stocks between land uses are low (≈ 28 Mg C ha−1) and concentrated in topsoils. The structure and species diversity of the forest partly explained the variability and the high C biomass compared to tree plantations. Type of forest and plantations is important to consider in conserving C stocks in landscapes.

Zusammenfassung: An nicht wenigen dichterischen Produktionen Goethes wirkt sprachgestalterisch eine gewisse Fülle von Wörtern und Konzepten sprachgeschichtlich fremden Ursprungs auffällig. Dieses Grundmerkmal goethescher Dichtung sucht die vorliegende Untersuchung näher zu beleuchten. In dieser Hinsicht wird durch ein positivistisch orientiertes Verfahren anhand bestimmter Dramenwerke eine Bestandsaufnahme dieser für die Sprachdiversität charakteristischen Sprachelemente systematisch erarbeitet und unter Bezug zu Goethes vielfachem Erfahren des Fremden untersuchend reflektiert.

Kersting’s groundnut (Macrotyloma geocarpum, Harms) is a legume crop with nutritional potential in West Africa where it is widely consumed due to its palatability. The present study investigated the physical characteristics, nutritional composition, and organoleptic properties of five Kersting’s groundnut accessions selected based on their yield performance, stability, and seed color. Physical characteristics (seed size, length, and thickness) were determined using a Vernier Caliper while a laboratory weighing scale was used to determine 100-seed weight. Protein and fat contents were analyzed using micro-Kjeldahl and Soxhlet systems, respectively. Dietary fiber content was determined using the AOAC method while carbohydrate content was determined using the method of difference. Amino acids were hydrolyzed and determined using the liquid chromatography method. Ten trained panelists participated in testing the cookability of the samples while 30 untrained panelists were involved in the acceptability test. Results showed that accessions ranged between 8.1 ± 0.1 and 9.3 ± 0.2 mm for seed length, 5.7 ± 0.4 and 6.0 ± 0.2 mm for the width, and 4.0 ± 0.0 and 4.9 ± 0.4 mm for thickness. The 100-seed weight ranged from 11.7 ± 0.8 to 16.7 ± 1.6 g. Protein, fat, and carbohydrate contents ranged from 20.5 ± 0.2 to 22.0 ± 0.2, 1.2 ± 0.0 to 2.3 ± 0.0, and 56.5 ± 0.0 to 59.4 ± 0.1 (g/100 g dw), respectively. The lowest and highest cooking times were 78 and 124 minutes, respectively. All accessions recorded good sensory acceptability (>50%) except the accession BUR 16 which was liked only by 23.3%. Overall, accession OUA had the highest protein, medium cooking time, and highest overall acceptability score (>70%), hence recommended for wide production. Future studies need to be performed on the antinutritional content of this accession and to find out the effect of cooking on the nutritional value of derived meals and starch modification.

Abstract Introduction To improve the diagnosis and survival of children living with HIV (CLWH), the World Health Organization recommends testing approaches beyond traditional infant HIV testing programmes. Information about undiagnosed HIV prevalence among children of varying ages in the general population is needed to guide innovative national/subnational case‐finding and testing approaches. Methods We used the Cost‐Effectiveness of Preventing AIDS Complications (CEPAC)‐Pediatric model to estimate the prevalence of undiagnosed HIV in 2‐, 5‐ and 10‐year‐old children in South Africa, Côte d'Ivoire and Zimbabwe in 2018. We simulated cohorts of children born in 2008 (10‐year‐olds), 2013 (5‐year‐olds) and 2016 (2‐year‐olds). Country‐/year‐specific inputs for pregnant/breastfeeding women included: HIV prevalence (4.2–32.3%), HIV incidence (0.03–0.24%/month), knowledge of HIV status (27–89%) and antiretroviral drug coverage (36–95%). Paediatric inputs included early infant testing coverage (6–95%) and breastfeeding duration (0–20 months). We projected the proportion of surviving CLWH in whom HIV remained undiagnosed and the undiagnosed HIV prevalence among surviving children of each age in the general population. For children born in 2016, we projected survival and diagnosis of all CLWH through 2026. We conducted sensitivity analyses on model parameters. Results In 2018, the projected proportion of surviving CLWH whose HIV remained undiagnosed in South Africa/Côte d'Ivoire/Zimbabwe was 44.2%/55.8%/52.9% among 2‐year‐old CLWH; 29.0%/37.8%/33.2% among 5‐year‐old CLWH; and 18.3%/25.4%/23.1% among 10‐year‐old CLWH. Projected general population undiagnosed HIV prevalence in South Africa/Côte d'Ivoire/Zimbabwe was 0.44%/0.32%/0.68% among 2‐year‐olds; 0.25%/0.17%/0.41% among 5‐year‐olds; and 0.24%/0.14%/0.38% among 10‐year‐olds. Among all CLWH born in 2016, 50–54% were projected to die without HIV diagnosis (and subsequently without treatment) within 10 years after birth; 80–85% of these deaths occurred in the first 2 years. Conclusions Projected population‐level undiagnosed HIV prevalence is low and sharply decreases after age 2, with more CLWH dying than being diagnosed. Despite low undiagnosed prevalence in the general population of older children, we project that a large proportion of CLWH remain undiagnosed, suggesting that innovative strategies targeting untested children of all ages outside of health facility settings should be prioritized. Programmes could consider routine testing of the general population of children below 2 in all settings and children of all ages in high‐prevalence settings.

IntroductionAccess to high-quality seeds remains a key constraint to the intensification of crop production in low-income countries. In this study, we analyzed maize seed production and distribution systems in Benin to identify leverage points for effective seed systems, a prerequisite for improving maize production.MethodsSemi-structured interviews were conducted with 81 seed producers selected in seven municipalities across the three phytogeographical zones of Benin. Key informant interviews were also conducted with ten public and private stakeholders involved in maize seed systems in Benin.Results and discussionFindings showed that the legal and institutional frameworks governing seed systems in Benin were recently reinforced with a national seed policy, the creation and operationalization of the National Committee of Plant Seeds and the existence of regulations and rules on the production, quality control, certification, trade, and packaging of seeds. In addition, enabling conditions to facilitate the involvement of the private sector have been greatly improved with the revision of modalities for obtaining approval for the production and distribution of seeds in Benin. While the seed sector is improving and both public and private stakeholders are involved in maize seed production and distribution, synergies among stakeholders need to be strengthened. Strengthening business and marketing skills of seed producers through training and promoting the comparative advantages of improved seeds in increasing yield and production among maize farmers could be a promising avenue. Connecting seed producers with maize farmers' organizations coupled with ICT-based agro-advisories could boost the development of the maize seed sector, and ultimately the maize value chain.

Abstract Herbaceous aboveground biomass (HAB) is a key indicator of grassland vegetation and indirect estimation tools, such as remote sensing imagery, increase the potential for covering larger areas in a timely and cost‐efficient way. Structure from Motion (SfM) is an image analysis process that can create a variety of 3D spatial models as well as 2D orthomosaics from a set of images. Computed from Unmanned Aerial Vehicle (UAV) and ground camera measurements, the SfM potential to estimate the herbaceous aboveground biomass in Sahelian rangelands was tested in this study. Both UAV and ground camera recordings were used at three different scales: temporal, landscape, and national (across Senegal). All images were processed using PIX4D software (photogrammetry software) and were used to extract vegetation indices and heights. A random forest algorithm was used to estimate the HAB and the average estimation errors were around 150 g m−² for fresh mass (20% relative error) and 60 g m−² for dry mass (around 25% error). A comparison between different datasets revealed that the estimates based on camera data were slightly more accurate than those from UAV data. It was also found that combining datasets across scales for the same type of tool (UAV or camera) could be a useful option for monitoring HAB in Sahelian rangelands or in other grassy ecosystems.

BackgroundHyperglycemia leads to serious damage to the body, especially the blood vessels and nerves. This study aimed to determine the prevalence and factors associated with hyperglycemia in a rural population of Tanvè and Dékanmey in Benin in 2019.Materials and methodsThis was a cross-sectional, descriptive and analytical study, nested in the Tanvè Health Study (TAHES) cohort. It covered all residents of the villages of Tanvè and Dékanmey, aged 25 years and above, and having given their written consent. Data were collected in the households during the fourth annual monitoring visit in 2019 using the WHO STEPS Wise approach. Hyperglycemia was defined as a fasting capillary blood glucose value ≥ 110 mg/dL. Data were analyzed with R Studio software version 3.5.1.ResultsA total of 1331 subjects were included in the study with a 60% female predominance and a sex ratio (male/female) of 0.7. The median age was 40 years (Q1 = 32 years; Q3 = 53 years) with a range of 25 and 98 years. The prevalence of hyperglycemia was 4.6%. In multivariate analysis, advanced age (AOR = 1.03; 95%CI = 1.02-1.73; p = 0.004), male sex (AOR = 2.93; 95%CI = 1.49-5.84; p = 0.023), monthly income> 105,000 FCFA (AOR = 2.63; 95%CI = 1.24-5.63; p = 0.030), abdominal obesity (AOR = 2.80; 95%CI = 1.29-6.16; p = 0.007, and obesity (AOR = 1.68; 95%CI = 0.75-3.59; p = 0.004) were statistically associated with hyperglycemia.ConclusionThe prevalence of hyperglycemia is not negligible in rural areas in Benin. Our study found that older age, male gender, high income, abdominal obesity, and obesity are determining factors in its occurrence.

Current diagnostic and surveillance systems in Benin are not designed to accurately identify or report non-Plasmodium falciparum (Pf) human malaria infections. This study aims to assess and compare the prevalence of circumsporozoite protein (CSP) antibodies of Pf and P. vivax (Pv) in Anopheles gambiae s.l. in Benin. For that, mosquito collections were performed through human landing catches (HLC) and pyrethrum spray catches (PSC). The collected mosquitoes were morphologically identified, and Pf, Pv 210, and Pv 247 CSP antibodies were sought in An. gambiae s.l. through the ELISA and polymerase chain reaction (PCR) techniques. Of the 32,773 collected mosquitoes, 20.9% were An. gambiae s.l., 3.9% An. funestus gr., and 0.6% An. nili gr. In An. gambiae s.l., the sporozoite rate was 2.6% (95% CI: 2.1–3.1) for Pf, against 0.30% (95% CI: 0.1–0.5) and 0.2% (95% CI: 0.1–0.4), respectively, for Pv 210 and Pv 247. P. falciparum sporozoite positive mosquitoes were mostly An. gambiae (64.35%), followed by An. coluzzii (34.78%) and An. arabiensis (0.86%). At the opposite, for the Pv 210 sporozoite-positive mosquitoes, An. coluzzii and An. gambiae accounted for 76.92% and 23.08%, respectively. Overall, the present study shows that P. falciparum is not the only Plasmodium species involved in malaria cases in Benin.

Entomological surveillance in Benin has historically been limited to zones where indoor residual spraying was performed or where long-standing sentinel surveillance sites existed. However, there are significant country-wide gaps in entomological knowledge. The National Malaria Control Program (NMCP) assessed population dynamics of Anopheles vectors and malaria transmission in each of Benin’s 12 departments to create an entomological risk profile. Two communes per department (24/77 communes) were chosen to reflect diverse geographies, ecologies and malaria prevalence. Two villages per commune were selected from which four households (HH) per village were used for human landing catches (HLCs). In each HH, an indoor and outdoor HLC occurred between 7 p.m. and 7 a.m. on two consecutive nights between July–September 2017. Captured Anopheles were identified, and ovaries were dissected to determine parous rate. Heads and thoraces were tested for Plasmodium falciparum sporozoites by ELISA. The Entomological Inoculation Rate (EIR) was calculated as the product of mosquito bite rate and sporozoite index. Bite rates from An. gambiae s.l., the primary vector species complex, differed considerably between communes; average sporozoite infection index was 3.5%. The EIR ranged from 0.02 infectious bites (ib) per human per night in the departments of Ouémé and Plateau to 1.66 ib/human/night in Collines. Based on transmission risk scales, Avrankou, Sakété and Nikki are areas of low transmission (0 < EIR < 3 ib/human/year), Adjarra, Adja Ouèrè, Zè, Toffo, Bopa, Pehunco, Pèrèrè and Kandi are of medium transmission (3 < EIR < 30 ib/human/year), and the other remaining districts are high transmission (EIR > 30 ib/human/year). The heterogeneous and diverse nature of malaria transmission in Benin was not readily apparent when only assessing entomological surveillance from sentinel sites. Prospectively, the NMCP will use study results to stratify and deploy targeted vector control interventions in districts with high EIRs to better protect populations most at-risk.

Abstract Background Changes in the natural habitats of insect groups are determined the genetic polymorphisms between individuals. The objective of this study was to establish the genetic structure of the Anopheles coluzzii populations in four localities of Benin. Methods Insecticide surveys and larval sampling were conducted on 4 study localities, including Cotonou, Ketou, Zagnanado, and Sô-Ava. Molecular characterizations were performed on the Anopheles mosquitoes collected with the allelic and genotypic frequencies of kdr gene determined. The multiple comparison Chi square test for proportions was performed with R version 3.3.3. Next, the observed heterozygosity, expected heterozygosity, and indices of fixation, and genetic differentiation were estimated. Finally, the Hardy–Weinberg equilibrium (EHW) was determined to assess whether panmixia exists in the different populations of mosquitoes of the agroecological zones under study. Results Carbamates, pyrethroids, organophosphorus and organochlorines use have been reported in all localities except Sô-Ava. Anopheles coluzzii was strongly represented across all study localities. The L1014F allele was observed in the localities of Kétou, Cotonou and Zagnanado. Likewise, insecticide selection pressure of homozygous resistant individuals (L1014F/L1014F) was significantly higher in Kétou, Cotonou and Zagnanado (p value

Abstract Background The fight against malaria faces various biological obstacles, including the resistance of parasites to anti-malarial drugs and the resistance of mosquito vectors to insecticides. The resistance of Anopheles gambiae sensu lato (s.l.) to pyrethroids, the only class of insecticides used to impregnate mosquito nets, is known in Benin; the expansion of this resistance is influenced by the existence of gene flow between species, otherwise by the presence or absence of the kdr mutation in them. The objective of this study is to determine the spatial distribution of An. gambiae and the level of expression of the pyrethroid resistance kdr gene in seven agro-ecological zones of Benin. Methods The study was conducted in 18 localities belonging to seven agro-ecological zones where environmental parameters varied. The sites represent the main areas of eco-epidemiological malaria in Benin. Anopheles gambiae larvae were collected in natural breeding sites using ladles and dipping method and reared under standard conditions. These larvae were reared under standard conditions of temperature and humidity (26 to 30 °C and 60 to 90%) at the insectarium of the Centre de Recherche Entomologique de Cotonou (CREC). Adult female mosquitoes having emerged are morphologically and molecularly identified. Homozygous resistant (1014F/1014F), homozygous sensitive (1014L/1014L) and heterozygous (1014F/1014L) genotypes of the L1014F kdr gene mutation are determined by PCR. Results A total of 677 An. gambiae was subjected at the PCR. The results revealed the presence of three vector species of the An. gambiae complex, of which 409 Anopheles coluzzii, 259 An. gambiae, 5 hybrids (An. coluzzii/An. gambiae) and 4 Anopheles arabiensis in the different agro-ecological zones. The four An. arabiensis were only found in Dassa, a locality in the cotton zone of central Benin. The frequency of distribution of the L1014F allele of the kdr gene varies from 84.48 to 100% in An. gambiae, from 80 to 100% in An. coluzzii and from 0 to 75% in An. arabiensis in the different agro-ecological zones. Moreover, a significant difference is generally observed in the distribution of the L1014F allele (P

Abstract Background Several studies carried out in Benin have shown the involvement of Anopheles gambiae sensu lato (s.l.), the Anopheles funestus group in malaria transmission, but none of them reported the contribution of the Anopheles nili group to the transmission of this disease. The current study investigated the question through an entomological cross-sectional survey performed in Northern Benin. Methods Mosquito samplings were performed in September and October 2017 in 4 villages located in two districts: Bambaba and Wodara (Kérou district) and, Péhunco 2 and Béké (Péhunco district). The collections were carried out indoors and outdoors using human landing catches (HLC) to assess the human biting rate (HBR) and pyrethrum spray catches (PSC) to evaluate the blood feeding rate and the blood meal origin using the ELISA test. All collected mosquitoes were morphologically identified and, the polymerase chain reaction (PCR) technique was used for molecular identification of sibling species of An. gambiae s.l., An. funestus group and An. nili group sporozoite index (SI) was also assessed by the ELISA test. Results Overall, An. gambiae s.l., An. funestus group and An. nili group were the three vectors found in the study area. A significantly higher human biting rate (HBR) was recorded in An. nili group (5 bites/human/night) compared to An. funestus group (0.656 bites/human/night) in the Kérou district (p

Abstract Background Several studies have reported the strong resistance of Anopheles gambiae s.l. complex species to pyrethroids. The voltage-dependent sodium channel (Vgsc) gene is the main target of pyrethroids and DDT. In Benin, the frequency of the resistant allele (L1014F) of this gene varies along the north-south transect. Monitoring the evolution of resistance is necessary to better appreciate the genetic structure of vector populations in localities subject to the intensive use of chemicals associated with other control initiatives. The purpose of this study was to map the distribution of pyrethroid insecticide resistance alleles of the Kdr gene in malaria vectors in different regions and ecological facies in order to identify the evolutionary forces that might be the basis of anopheline population dynamics. Methods The characterization of Anopheles gambiae s.l. populations and resistance mechanisms were performed using adult mosquitoes obtained from larvae collected in the four agroecological zones in southern Benin. Genomic DNA extraction was performed on whole mosquitoes. The extracted genomic DNA from them were used for the molecular identification of species in Anopheles gambiae s.l. complex and the identification of genotypes related to pyrethroid resistance as the Kdr gene amino acid position 1014 in sodium channel. Molecular speciation and genotyping of Kdr resistant alleles (1014) were done using PCR. Genepop software version 4.2 was used to calculate allelic and genotypic frequencies in each agroecological zone. The p value of the allelic frequency was determined using the binomial test function in R version 3.3.3. The Hardy-Weinberg equilibrium was checked for each population with Genetics software version 1.3.8.1. The observed heterozygosity and the expected heterozygosity as well as the fixation index and genetic differentiation index within and between populations were calculated using Genepop software version 4.2. Results During the study period, Anopheles coluzzii was the major species in all agroecological zones while Anopheles gambiae was scarcely represented. Regardless of the species, resistant homozygote individuals (L1014F/L1014F) were dominant in all agroecological zones, showing a strong selection of the resistant allele (L1014F). All populations showed a deficit of heterozygosity. No genetic differentiation was observed between the different populations of the two species. For Anopheles coluzzii, there was a small differentiation among the populations of the central cotton and bar-lands zones. The genetic differentiation was modest among the population of the fisheries zone (Fst = 0.1295). The genetic differentiation was very high in the population of Anopheles gambiae of the bar-lands zone (Fst = 0.2408). Conclusion This study revealed that the use of insecticides in Benin for years has altered the genetic structure of Anopheles gambiae s.s. populations in all agroecological zones of southern Benin. It would be desirable to orientate vector control efforts towards the use of insecticides other than pyrethroids and DDT or combinations of insecticides with different modes of action.

Quality, shelf life, and yield of a pineapple fruit are the important attributes for the producers and customers in the pineapple value chain of Benin, whereas poor quality, short shelf life, and low yield are the main constraints. We quantified the effects of planting density and K2O:N fertilizer ratio on the pineapple yield, external quality, and perceived shelf life in four on-farm experiments with cv. Sugarloaf in Benin; two experiments were installed in the long rainy season and two in the short rainy season. A split-plot design was used with the planting density as the main factor at three levels: 54,000, 66,600, and 74,000 plants.ha−1. The K2O:N ratio was a subfactor with three levels: K2O:N = 0.35 (farmers' practice), K2O:N = 1, and K2O:N = 2. The results showed that both factors had no effect on the crop development variables (such as the number of functional leaves and D-leaf length) at the moment of flowering induction. The planting density had no effect on the total weight per fruit, infructescence weight, total fruit length, infructescence length, crown length, or the fruit shelf life as perceived by traders. The yield increased from 54.9–69.1 up to 90.1 t.ha−1 with an increase in the planting density. The yield increase was not at the expense of the fruit weight. Increased K2O:N ratio led to a higher fruit weight whereas the fruit length was not affected. The shelf life of fruits produced at a K2O:N ratio of 1 and as perceived by traders was 6 days longer than that of fruits produced at a ratio of 0.35 (farmers' practice). Based on these results, we suggest the fresh pineapple farmers in Benin to use a combination of 66,600 plants.ha−1 with a K-fertilization scheme based on a K2O:N ratio of 1 to meet the expectation of both producers and customers in terms of fruit yield and fruit quality.

Abstract Background In recognition of the threat of insecticide resistance in vectors of malaria, the WHO Global Malaria Programme recommends the development of an appropriate and comprehensive response to insecticide resistance. In principle, good resistance management practice requires the application of multiple insecticides of different modes of action, for example, in rotations and mixtures. Insecticides recommended by the World Health Organization for indoor residual spraying and long-lasting insecticide nets are limited. It is, therefore, judicious to prevent the rapid spread of insecticide resistance by evaluating new insecticides formulations with different modes of action and long residual effect. Methods Fludora® Fusion, a new neonicotinoid IRS formulation (a mixture of 500 g/kg clothianidin and 62.5 g/kg deltamethrin applied 200 mg ai/sqm + 25 mg ai/sqm, respectively) was tested. Small scale field evaluation of this product was conducted in the district of Dangbo in Benin, to compare its efficacy and residual effect on cement and mud walls against those of clothianidin 200 mg ai/sqm (WG 70) alone, and of deltamethrin 25 mg ai/sqm (WG 250) alone. WHO wall cone bioassays were conducted monthly with laboratory susceptible Anopheles “Kisumu” and wild Anopheles gambiae sensu stricto (s.s.) population from Dangbo. The induced mortality by each treatment per wall substrate for 24 h, 48 h, and 72 h post exposure were recorded every month and analysed. Results Fludora® Fusion and clothianidin WG 70 showed mortality rates over 80% WHO bio-efficacy threshold on cement walls either with susceptible or resistant An. gambiae s.s. over a period of 10 and 9 months, respectively. Treatment with Fludora® Fusion and clothianidin WG 70 on the mud walls showed residual effect for 6 months and 5 months respectively against both susceptible and resistant mosquitoes. During the whole evaluation period, deltamethrin WG 250 showed mortality rates below 80% against resistant Anopheles population. Furthermore, the knock down rates observed with the Fludora® Fusion combination were significantly higher (p

Abstract Background Scale-up of the distribution of long-lasting insecticide-treated bed nets and indoor residual spraying with insecticides over the last decade have contributed to the considerable decrease of malaria morbidity and mortality in sub-Saharan Africa. Due to the increasing pyrethroid resistance intensity and the spread of carbamate resistance in Anopheles gambiae (s.s.) mosquitoes and the limited number of insecticides recommended by the WHO for vector control, alternative insecticide formulations for IRS with long-lasting residual activity are required to sustain the gains obtained in most malaria-endemic countries. Methods SumiShield 50WG (clothianidin 300 mg ai/m2) developed by Sumitomo Chemical was evaluated alongside deltamethrin 25 mg ai/m2 (K-Othrine 250 WG) against a pyrethroid resistant Anopheles gambiae (s.l.) population in experimental huts in Covè, Benin. Residual activity was also tested in cone bioassays with the susceptible An. gambiae “Kisumu” strain and the local wild resistant population. Results The results showed very low toxicity from deltamethrin (mortality rates ranged between 1–40%) against host-seeking resistant Anopheles populations. SumiShield in contrast gave an overall mean mortality of 91.7% at the 120 h observation across the eight- month observation period following spraying. The residual activity measured using cone tests was over the 80% WHO threshold for 24 weeks for resistant wild Anopheles population and 32 weeks for the susceptible strain “Kisumu” after the spraying. Conclusions SumiShield is a good candidate for IRS in areas of permanent malaria transmission and where Anopheles populations are resistant to other conventional insecticides such as pyrethroids. It would be interesting to complete experimental huts studies by assessing the efficacy and residual effect of SumiShield 50WG at community level (small-scale field testing) in an area where vectors are highly resistant to insecticides.

Despite the growing interest in the miracle plant worldwide due to its numerous applications, the threats and the wild harvest of the species hamper its sustainable utilisation. Moreover, traditional knowledge so far documented on the species is limited to a narrow geographical coverage of its natural distribution range, which is West and Central Africa. This study analysed the use variation and knowledge acquisition pattern of the miracle plant among West African sociolinguistic groups and deciphered the drivers of populations’ willingness and readiness to engage in cultivating the species. Semi-structured interviews were conducted with 510 respondents purposively selected from nine sociolinguistic groups in Benin and Ghana using the snowball sampling approach. Information was collected on respondents’ socio-demographic profile, miracle plant ownership, plant parts used and preparation methods, knowledge of the species bioecology, perceived threats on the species, willingness to cultivate, maximum acreage to allocate to the species and maximum price to pay for a seedling. Descriptive statistics, generalized linear models, classification and regression tree models were used for data analysis. The miracle plant ownership mode depended on the age category. Sociolinguistic affiliation, level of schooling, migratory status and religion significantly affected the number of trees owned. We recorded 76 uses belonging to six use categories. The overall use-value of the miracle plant significantly varied according to the respondent sociolinguistic affiliation, main activity and religion. Men were the main source of knowledge and knowledge is mainly acquired along the family line. Knowledge related to food and social uses was mostly acquired from parents and people of the same generation, while magico-therapeutic and medicinal use-related knowledge were inherited from parents and grandparents. Sociolinguistic affiliation, awareness of taboos and market availability were the most important drivers of respondent willingness to cultivate the miracle plant. While the respondent’s level of schooling and perception of plant growth rate determined the maximum acreage they were willing to allocate to the species in cultivation schemes, their main activity, sociolinguistic affiliation and knowledge of the species time to fruiting drove the maximum purchase price they were willing to offer for a seedling of the species. Our findings provide key information for the promotion of miracle plant cultivation in the study area.

Abstract Background Mango is an important tree fruit in Sierra Leone since it participates to food and nutritional security mainly in rural areas. However, the economic potential of this tree fruit is still untapped to a large extent. This situation is due to high loss and poor handling at post-harvest stage preventing farmers from meeting the quality standard for different markets. The aims of this paper were to analyse the constrains and opportunities for mango value chain development, as well as community-based copping strategies developed by chain actors to valorise the existing opportunities for better market development and forest conservation. Methods Data were collected in four sites around the Outamba Kilimi National Park, Sierra Leone, through focus group discussions with men and women groups of 25–35 mango value chain actors in each site. Results Results indicated that mango value chain is composed of nursery suppliers, mango producers, harvesters and assemblers, processors and traders. Main constraints encountered by both men and women are lack of: (1) polythene bags and improved mango seedlings, mineral fertilisers, herbicides, pesticide, (2) training on appropriate mango production practices, (3) appropriate harvesting tools, (4) appropriate processing knowledge and equipment, as well as high transportation cost. Hand weeding, use of available organic fertiliser, seeking of advices from colleagues farmers, equipment from other processors who own local processing equipment were some of the coping strategies developed by various actors along mango value chains. Soil fertility, favourable climatic conditions, technical and financial support from direct or indirect actors through training on good agronomic practices, processing and marketing techniques, availability of hired labour to perform farming and processing operations, existence of local processing plant to serve as outlet for fresh mango were identified as main factors to be exploited to improve mango value chains around Outamba Kilimi National Park in Sierra Leone. Conclusions Our results provided insight into the various factors limiting the performance of mango value chains around Outamba Kilimi National Park in Sierra Leone. This baseline study will help in designing and implementing appropriate strategies to promote mango value chains in the areas around the Outamba Kilimi National Park.

Abstract Background Malaria control is heavily reliant on insecticides, especially pyrethroids. Resistance of mosquitoes to insecticides may threaten the effectiveness of insecticide-based vector control and lead to a resurgence of malaria in Africa. Methods In 21 villages in Southern Benin with high levels of insecticide resistance, the resistance status of local vectors was measured at the same time as the prevalence of malaria infection in resident children. Results Children who used LLINs had lower levels of malaria infection [odds ratio = 0.76 (95% CI 0.59, 0.98, p = 0.033)]. There was no evidence that the effectiveness of nets was different in high and low resistance locations (p = 0.513). There was no association between village level resistance and village level malaria prevalence (p = 0.999). Conclusions LLINs continue to offer individual protection against malaria infection in an area of high resistance. Insecticide resistance is not a reason to stop efforts to increase coverage of LLINs in Africa.