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The influence of different strawberry varieties on the microbiological, antioxidant and sensory properties of probiotic yoghurt, containing Bifidobacterium animalis subsp. lactis Bb12, were monitored for 21 days. For this purpose, strawberry pulp (15 %) containing strawberry and sugar in a 1:1 ratio was used in the yoghurt formulation. Higher total phenolic content and ferric reducing antioxidant power (FRAP) were detected in Albion and Camarosa varieties than Rubygem whereas Albion variety had the highest total anthocyanin content (p

Bu çalışmada, modifiye atmosfer paketleme (MAP) ve 1-metilsiklopropen (1-MCP) ‘Farfia’ kayısı çeşidi meyvelerinin hasat sonrası dayanımlarına etkilerinin belirlenmesi amaçlanmıştır. Çalışma; a) Kontrol, b) MAP, c) 1-MCP, d) MAP + 1-MCP, e) RipeLock™ MAP ambalaj + 1-MCP olacak şekilde beş farklı uygulama gerçekleştirilmiştir. 1-MCP, 24 saat 625 ppb olarak uygulanmıştır. ‘Farfia’ kayısı çeşidine ait meyveler 8 hafta süreyle 0°C’de %90 nemde depolanmıştır. Depolama öncesi ve süresince 2 haftalık periyotlarla alınan örneklerde ambalajların ağzı açılarak 2 gün raf ömründe (20°C) bekletildikten sonra ağırlık kaybı, renk, meyve eti sertliği, suda çözünür kuru madde miktarı, asitlik, pH, toplam fenol miktarı, antioksidan aktivitesi, solunum hızı, etilen salınımı, duyusal değerlendirme ve çürüklük gelişimi belirlenmiştir. MAP’ın yer aldığı uygulamalar, kayısı meyvelerinin ağırlık kaybını depolama ve raf ömrü süresince önemli derecede azalttığı görülmüştür. Meyve eti sertliğinin korunmasında, MAP ile 1-MCP’nin birlikte uygulamaları etkili olmuştur. MAP’ın yer aldığı uygulamalarda meyvelerin solunum hızları daha düşük bulunmuştur. MAP ve 1-MCP’nin teksel ve birlikte uygulanmaları kayısı meyvelerinin etilen salınımını yavaşlatmıştır. Çalışma sonucunda MAP ile 1-MCP’nin birlikte yapıldığı uygulamalardaki ‘Farfia’ kayısı meyvelerinin 42 gün başarıyla saklanabileceği saptanmıştır.

This study was carried out to determine the effects of different openings onto the polyethylene (PE) packages used in storage of ‘Sultani Çekirdeksiz’ grape variety on quality, SO2 damage, pathological and physiological disorders of grapes. Grapes were harvested at full maturity and placed in PE bags with ‰0 (control), ‰0.5, ‰1 and ‰2 openings. After pre-cooling grapes in the case of SO2 pets were placed after the mouth of the bags were closed. Grapes were stored at 0°C and 90-95% relative humidity for 4 months, and quality changes were determined in monthly samples. SO2 concentrations detected at the end of storage in PE packages with ‰0, ‰0.5, ‰1 and ‰2 openings were 11.16, 5.80, 2.05 and 0,00 mg/kg respectively. It has been found that grapes in packages without opening had significant SO2 content and SO2 damage, especially the end of storage. However, grapes in packages with ‰2 openings had high weight loss and browning scores in stems. The results showed that ‘Sultani Çekirdeksiz’ grapes could be stored more successfully in PE packages with ‰0.5 openings. ‘Sultani Seedless’ grape variety, it may be suggested to use PE bags without openings for 2 and 3 months storage.

Due to the soft texture of the nectarine fruit, there are difficulties in long-term storage of this fruit. Therefore, it is of great importance to extend the postharvest storage period of this fruit species. In this study, the effect of postharvest OA (Oxalic acid) applications (0, 0.5, 1 and 1.5 mM) on the quality and biochemical contents of nectarine fruits was investigated. On the 40th day of storage, 1.5 mM OA doses reduced weight loss (1.96%) and fruit flesh firmness (36 N) by approximately 44% and 20%, respectively, compared to the control group. The most effective dose in reducing the respiratory rate was again 1.5 mM OA. The 1 mM OA dose was determined to prevent the decay rate approximately by 16% compared to the control group. Organic acid contents showed a continuous decreasing trend during storage and malic acid was determined to be the dominant acid in nectarine fruits. At the end of storage, it was determined that a 1.5 mM OA dose prevented the decrease in malic acid content approximately 35% more than the control group. Chlorogenic acid and rutin were detected at a higher rate than other phenolics. It was observed that 1.5 mM OA dose prevented the breakdown of chlorogenic acid and rutin compounds more so than other doses during storage. In this study, it was determined that a 1.5 mM OA dose especially protected the quality properties and biochemical contents of nectarine fruits in the cold storage more than the control group.

Free radicals are chemical molecules that are more reactive and have an unpaired electron. Free radicals formed inside the cell oxidize biomolecules, leading to cell death and tissue damage. Antioxidants are molecules that can stabilize or inactivate free radicals before they damage the cell. In this study; the availability of Malondialdehyde, Superoxide dismutase, Catalase and Reduced glutathione levels as markers for related diseases was evaluated by examining whether and in what range they may vary in some diseases. In study, nine groups consist of prostate cancer, cirrhosis, liver transplantation, chronic kidney damage, acute kidney injury, X-ray exposure, CT exposure, MR exposure and Osteonecrosis. Analysis of means is a method developed to compare group means with the overall mean and presents the results graphically in an easy-to-understand manner without the required for any post hoc test. In addition, related characteristics were categorized as “low and high” and Nonlinear Principal Component Analysis was conducted to visually present their relationship with related disease types in two-dimensional space. The upper and lower decision lines were found 3.123 and 2.794 μmol/L, respectively for Malondialdehyde. Those with cirrhosis, chronic kidney disease, acute kidney disease and tomography exposure were included in the upper and lower decision lines. Those with prostate cancer, osteonecrosis, and X-ray exposure were above the upper decision line and are found higher than the overall mean. Those with lung transplantation and MR exposure appear to be below the lower decision line and lower than the overall mean. The present study provides the first comprehensive assessment of the availability of Malondialdehyde, Superoxide dismutase, Catalase and Reduced glutathione levels as markers for some related diseases. This study has shown that Analysis of means can be used as an alternative graphical procedure for multiple group comparisons with an overall mean in the studies regarding as biochemical characteristics and relating diseases. In addition, Nonlinear Principal Component Analysis can be useful aid for decision marker in some biochemical characteristics and related diseases.

In this study, it was aimed to determine the effects of gibberillic acid (GA3) applications on drop and quality changes of fruits on-tree storage period. GA3 (before peel color break, on the peel color break and both periods) and 20 ppm GA3 (before peel color break and on the peel color break) were applied to the trees, and the untreated trees constituted the control group. Fruits were harvested in five periods at monthly intervals from april. In the last harvest period, the average fruit drop rate was significantly higher in the control group (38.48%) than in the color transformation 10 ppm GA3 (9.44%) and before color transformation 20 ppm GA3 (19.28%). The fruit drop was not observed in the first and second harvest periods. Especially in the first period on-tree storage, the shell C* and h° values of the fruits in control were found higher than the GA3 application. As a result, twice application of 10 ppm GA3 in 'Valencia Late' orange reduced pre-harvest fruit drop, delayed peel color transformation and limited fruit quality change. Accordingly, it has been revealed that it allows on-tree storability until the end of august.

The storage and shelf life of the fig, which has a sensitive fruit structure, is short, and this results in excessive economic losses. In a study carried out to contribute to the solution of this problem, the effect of postharvest putrescine application at different doses (0, 0.5, 1.0, 2.0, and 4.0 mM) on fruit quality characteristics and biochemical content during cold storage in figs was determined. At the end of the cold storage, the decay rate and weight loss in the fruit were in the ranges of 1.0–1.6% and 1.0–5.0 %, respectively. The decay rate and weight loss were lower in putrescine-applied fruit during cold storage. Putrescine application had a positive effect on the changes in fruit flesh firmness values. The SSC rate of fruit varied between 14 and 20%, while significant differences in the SSC rate occurred depending on storage time and putrescine application dose. With putrescine application, the decrease in the acidity rate of the fig fruit during cold storage was smaller. At the end of the cold storage, the acidity rate was between 1.5–2.5% and 1.0–5.0. Putrescine treatments affected total antioxidant activity values and changes occurred in total antioxidant activity depending on the application dose. In the study, it was observed that the amount of phenolic acid in fig fruit decreased during storage and putrescine doses prevented this decrease. Putrescine treatment affected the changes in the quantity of organic acids during cold storage, and this effect varied depending on the type of organic acid and the length of the cold storage period. As a result, it was revealed that putrescine treatments can be used as an effective method to maintain postharvest fruit quality in figs.

This paper report is an analysis of the title compound by means of X-ray crystallography, FT-IR, NMR and DFT calculations, in the context of structural and spectral characterization. The crystal and molecular structures of the compound were determined by single-crystal X-ray diffraction (SCXRD). Fourier Transform Infrared (FTIR) spectrum was recorded in the range from 400 cm–1 to 4000 cm–1. The 1H and 13C nuclear magnetic resonance (NMR) spectra were also recorded. DFT calculations were employed to support X-ray molecular geometry and calculate IR and NMR (1H and 13C) spectral bands. The structural (bond lengths, bond angles, torsion angles) and spectral (vibrational modes and chemical shifts) parameters obtained from DFT levels (B3LYP/6-31G(d,p) and B3LYP/6-31G+(d,p)) were compared with experimental findings, and an excellent harmony between the two data was ascertained.

The title compound was synthesized and structurally characterized. Theoretical IR, NMR (with the GIAO technique), UV, and nonlinear optical properties (NLO) in four different solvents were calculated for the compound. The calculated HOMO–LUMO energies using time-dependent (TD) DFT revealed that charge transfer occurs within the molecule, and probable transitions in the four solvents were identified. The in silico absorption, distribution, metabolism, and excretion (ADME) analysis was performed in order to determine some physicochemical, lipophilicity, water solubility, pharmacokinetics, drug-likeness, and medicinal properties of the molecule. Finally, molecular docking calculation was performed, and the results were evaluated in detail.

Abstract The composite consisted of clay and polymers like polyethylene (GCP) was used to remove methylene blue (MB) from the water. The most effective pH, temperature and initial dye concentration in adsorption process were found to be 9, 55 °C and 5 × 10−6 M, respectively. The results of the experiment showed that the adsorption process was compatible with the pseudo-second-order model. Activation parameters of ΔG: − 70.64 K J mol−1, ΔS: − 70.64 J mol−1 K−1, E a: 12.37 K J mol−1 at 308 °C were calculated and showed that adsorption process was exothermic and spontaneous. The results revealed that adsorption of MB on composite GCP was spontaneous and the composite of GCP f could be used for removing of MB from the water.

Abstract In this research, adsorption properties of bovine serum albumin (BSA) on diatomite clay, which is an oxide mineral, were studied as a function of BSA, sodium phosphate buffer and protein concentration and pH and the thermodynamic parameters of adsorption process were investigated. The BSA adsorption experiment onto diatomite clay indicated that the BSA solution reached the maximum adsorption value at pH 5.5. It was observed that the maximum adsorption capacity (qm) of the data obtained from the adsorption studies showed a great dependence on pH. The maximum amount of adsorption in adsorption experiments can be considered as points where the electrostatic interaction for pH is appropriate. Both structural and electrostatic interaction in regions outside of the isoelectric point may have caused a decrease in BSA absorbance. The structural influences were associated with different conformational states that while BSA molecules accept changes with pH, electrostatic effects can be observed in BSA molecules behaved like soft particles. In this case, it is not possible to explain the independence of the qm–pH curves of the amount of adsorption. The protein molecules at this point are very stable. Because this value is close to the isoelectric point of serum albumin. The surface structural change of BSA and diatomite clay was studied. For this, Fourier transform infrared spectroscopy (FTIR) spectroscopy values were compared before and after the experiment. The diatomite samples used as support material were characterized by FTIR, scanning electron microscopy, thermogravimetric analysis and Brunauer Emmett–Teller surface area analysis. The thermodynamic functions such as enthalpy, entropy, Gibbs free energy and activation energy were investigated in their experimental work. The thermodynamic parameters such as Gibbs free energy (ΔG*), E a, ΔH* and ΔS* were calculated as − 67.45, 15.41, − 12.84 kJ mol−1 and − 183.28 J mol−1 K−1 for BSA adsorption, respectively. We can deduce that the adsorption process from the data obtained from the thermodynamic parameters is spontaneous and exothermic. The adsorption of the process was investigated using Eyring and Arrhenius equations, and its adsorption kinetic found to be coherent with the pseudo-second-order model. As a result, we reached that the diatomite clay is a suitable adsorbent for the BSA. Experimental results showed that diatomite clay has the potency to be used for rapid pretreatment in the process of identifying proteins.

The brown (Kumato) tomato is distinguished by its distinctive 'brown' appearance and sweet aroma, unlike the usual red tomatoes. In this study, quality changes of KM8367, KM8034 and KM1210 Kumato tomato cultivars grown in the plastic greenhouse by soilless culture method were investigated during storage. Tomato fruits harvested at commercial maturity stage were stored in cardboard boxes at 7°C and 85-90% relative humidity for 21 days. Some physical, chemical and biochemical analyses were carried out at the beginning of storage and 7 days intervals during storage period. During storage, fruit weight, width and height in KM8367, total soluble solids, total phenol amount and antioxidant activity in KM1210 were found high, and fruit firmness and TA amounts in KM8034 variety were found low. Changes in color values (C*, h°) of Kumato tomato varieties were similar during storage. It was determined that the effect of storage time on quality parameters of Kumato tomato fruits was limited. These varieties have been shown to protect marketable qualities at the end of 21 days of storage.

In the present study, a combined experimental and computational study on molecular structure and spectroscopic characterization on the title compound has been reported. The crystal was synthesized and its molecular structure brought to light by X-ray single crystal structure determination. The spectroscopic properties of the compound were examined by FT-IR and NMR (1H and 13C) techniques. FT-IR spectra of the target compound in solid state were observed in the region 4000–400 cm−1. The 1H and 13C NMR spectra were recorded in CDCl3 solution. The molecular geometries were those obtained from the X-ray structure determination optimized using the density functional theory (DFT/B3LYP) method with the 6-31G(d, p) and 6-31G+(d, p) basis set in ground state. From the optimized geometry of the molecule, geometric parameters (bond lengths, bond angles and torsion angles), vibrational assignments and chemical shifts of the title compound have been calculated theoretically and compared with those of experimental data. Besides, molecular electrostatic potential (MEP), frontier molecular orbitals (FMOs), Mulliken population analysis, Thermodynamic properties and non-linear optical (NLO) properties of the title molecule were investigated by theoretical calculations.

Objective: Despite recent advances in medical support and interventions, only 5% to 10% of patients with out-of-hospital cardiac arrest (OHCA) survive to discharge. In this study, factors related to neurologically favorable survival in patients with OHCA were analyzed. Methods: A total of 129 patients who were admitted to hospital with OHCA were retrospectively enrolled. Results: Sustained return of spontaneous circulation (ROSC) (ROSC lasting >20 min) was achieved in 29 (22.4%) patients. Percentage of cardiac arrests with ischemic etiology was significantly higher in successful ROSC group (p

Pesticides cause pollution by remaining in water, soil, fruits and vegetables for a long time and also reach human through the food chain. It was thought that some pesticides used in agriculture could adversely affect the antioxidant enzyme system and the minimum inhibition values were studied. glutathione s-transferase (GST), an important antioxidant enzyme, catalyzes the conjugation of glutathione with toxic metabolites. It was purified from the blueberry fruits. The purification of the enzyme was performed separately by affinity and gel filtration chromatography. The purity of the enzyme was determined by SDS-PAGE electrophoresis. Characterization studies were done for the enzyme. For this purpose, optimal pH, temperature, Km and Vmax values for GSH and CDNB were also determined for the enzyme as 7.2 in K-phosphate buffer, 50 °C, 1.0 M, 7.0 in K-phosphate buffer, 1.57 mM; 0.17 mM and 0.048 EU/mL, 0.0159 EU/mL, respectively. Additionally, inhibitory effects of some pesticides; dichlorvos, acetamiprid, cyhalothrin, haloxyfop-p-Methyl, 2,4 dichlorophenoxy acetic acid, cypermethrin, imidacloprid, fenoxaprop-p-ethyl, glyphosate isopropylamine salt were examined the enzyme activity in vitro by performing Lineweaver–Burk graphs and plotting activity % IC50 and Ki values were calculated for each of pesticides. All of the pesticides inhibited the GST enzyme at millimolar level. Pesticide showing the best inhibitory effect was found as dichlorvos. The Ki value which is the inhibition constant of this pesticide was 0.0175 ± 0.005.

Yumurta kolisi (viyol) imalatının en önemli süreçlerinden biri kurutma işlemidir. Viyol hamuru kalıplara döküldükten sonra yapısında bulunan su moleküllerini yok etmek için kurutma işlemi gerçekleştirilmektedir. Endüstride viyol kurutma işlemi geleneksel kurutma sistemleri ile yapılmaktadır. Yapılan bu çalışmada ise; özel olarak tasarlanıp imal edilmiş prototip konveyörlü mikrodalga fırın sisteminde ıslak viyolün kurutma deneyleri yapılmıştır. Deneyler sonucunda, magnetron gücünün artmasıyla nem oranının düştüğü, dolayısıyla viyolün kurutma süresinin azaldığı görülmüştür.

Yumurta kolisi (viyol) imalatının en önemli süreçlerinden biri kurutma işlemidir. Viyol hamuru kalıplara döküldükten sonra yapısında bulunan su moleküllerini yok etmek için kurutma işlemi gerçekleştirilmektedir. Endüstride viyol kurutma işlemi geleneksel kurutma sistemleri ile yapılmaktadır. Yapılan bu çalışmada ise; özel olarak tasarlanıp imal edilmiş prototip konveyörlü mikrodalga fırın sisteminde ıslak viyolün kurutma deneyleri yapılmıştır. Deneyler sonucunda, magnetron gücünün artmasıyla nem oranının düştüğü, dolayısıyla viyolün kurutma süresinin azaldığı görülmüştür.

Child abuse is a global health problem. For this reason, it is important to perform suitable medical approaches in suspected cases of child abuse in the emergency room. The first consideration is performing life-saving medical approaches. Reporting the case to judicial authorities and the correlation of medical examination findings with medicolegal history are other important steps. We report a case of a 3-year-old male child who died after severe physical abuse. In suspected cases of child abuse, examination of the fundus is important. If death occurs, histopathological examination of eye globes must be performed to determine the cause of death. The present case was the first in which eye globe examination was performed in Izmir, Turkey. Autopsy findings revealed subdural hemorrhage and subarachnoid hemorrhage caused by blunt trauma. The cause of death was blunt head trauma, and the manner of death was listed as homicide. Except for microhemorrhages in the optic nerve sheath, no pathological finding was identified in the retina. Retinal hemorrhage can be seen in child abuse cases, and it is associated with a poor prognosis if present; however, the absence of retinal hemorrhage does not exclude child abuse. Crime scene investigation findings, witnesses’ statements, medical records, and autopsy findings must all be taken into consideration in child abuse cases in a correlative and holistic manner.

In the title compound, [Cu(NO3)2(C5H6N2)4], the CuII ion is located on an inversion centre. It features a Jahn–Teller-distorted octahedral coordination geometry, defined by four N atoms of four 1-vinylimidazole ligands in the equatorial plane and two nitrate O atoms in the axial positions. The nitrate anion is disordered over two sets of sites in a 0.801 (6):0.199 (6) ratio. In the crystal, the complex molecules are linked by weak intermolecular C—H...O and C—H...π interactions.

In the title Schiff base compound, C13H9Cl2NO, the molecule displays an E conformation about the imine C=N double bond, with a dihedral angle of 8.09 (11)° between the two benzene rings. In the crystal, molecules are linked by a single O—H...O hydrogen bond, giving one-dimensional chains which extend along (100).

The title molecule, C26H35NO2, contains two cyclobutane rings that adopt butterfly conformations and are linked by a –CH(OH)CH2NHCH2CH(OH)– bridge. In the crystal, N—H...O, O—H...N and O—H...O hydrogen bonds together with C–H...π interactions link the molecules.

In the title compound, C20H30N2O2, the cyclobutane ring is puckered, with a dihedral angle of 19.60 (13)° between the two planes. In the crystal, the molecules are linked by intermolecular O—H...N and weak C—H...O hydrogen bonds, as well as a C—H...π hydrogen-bonding association.

Adli soruşturmalar; adli tahkikat, olay yeri incelemeleri, otopsi ve laboratuvar bulgularını kapsar. Ölüm sebebinin saptanması, elde edilen tüm bilgilerin dikkatlice toparlanmasına dayanır. Otopsi yapılmaksızın, adli tahkikata dayanarak ölüm sebebinin belirtilmesi ve defin ruhsatı düzenlenmesi sonucunda; kararın yeniden gözden geçirilmesinin gerektiği durumlar ortaya çıkabilir. Bu yazıda; bir inşaatın zemininde bulunan cesedin, savcılık tarafından yapılan olay yeri incelemesi sırasındaki görüşmelerde; kişinin iş kazası sonucu kafa travmasına bağlı olarak öldüğü ifade edilmiştir. Yapılan otopside ise; kişinin av tüfeği yaralanması sonucu meydana gelen yaralanmalar nedeniyle öldüğü belirlenmiştir. Bu olgu, adli olayların sağlıklı çözümünde yaklaşımların önemini vurgulamak amacıyla sunulmaktadır. Anahtar Kelimeler: Av Tüfeği Yaralanması, Ölüm Nedeni, Olay Yeri Keşfi, Adli Soruşturma, Otopsi.

Abstract Background Owing to its high perishability, the market life of nectarine fruit is very short. Cold storage is a principal approach to limit post-harvest quality loss in nectarines. The objective of this research was to evaluate the impact of postharvest methyl jasmonate (MeJA), salicylic acid (SA) and 1-methylcyclopropene (1-MCP) on quality properties of nectarine fruit, specifically weight loss, firmness, phenolics and antioxidant activity, following cold storage and subsequent shelf life. Fruit immersed in water were considered as control. The fruit were stored at 0 ± 0.5 °C and 90 ± 5% RH for 56 d, then kept at 20 ± 0.5 °C and 70 ± 5% RH for 2 d in shelf life. Results The results demonstrated that single or combined treatments of MeJA, SA and 1-MCP were effective on quality characteristics. During cold storage, fruit treated with MeJA + 1-MCP (3.66%) and SA + 1-MCP (3.54%) exhibited lower weight loss than the control (4.08%). In the final two measurements of storage, the flesh firmness of fruit treated with SA + 1-MCP (54.5 and 54.06 N, respectively) was higher than that of the control. At the end of cold storage, the SA + 1-MCP treatment (17.4%) exhibited higher soluble solids than the control (15.37%) and SA (15.20%) treatments. However, the total phenolics content was found to be higher in nectarine fruit treated with single SA than in the control, as well as in fruit treated with SA + 1-MCP and single 1-MCP. Wooliness in fruit treated with 1-MCP, SA and MeJA was found to be lower than in the control, while fruit had higher acceptance. Conclusions As a result, the SA + 1-MCP and MeJA + 1-MCP treatments were more efficacious in retarding the weight and firmness decline of nectarine fruit during storage. Also, it was revealed that 1-MCP, SA and MeJA could be employed as efficacious instruments in nectarine fruit with respect to wooliness and acceptance, which influence consumer preferences.

Abstract Background Post-harvest losses cause significant product losses in the world, which leads to food waste. Therefore, it is of great importance for people to have access to sufficient amounts of products by increasing the storage period of fruits with applications such as post-harvest calcium chloride (CaCl2). In this study, the effect of calcium chloride (CaCl2) on physical quality parameters and bioactive contents of stored strawberry fruit (Albion cv.) was investigated. Accordingly, strawberries were treated with 2%, 4% and 6% CaCl2 before storage and stored for 15 days (0 ± 0.5 °C and 90 ± 5% RH). Analyses and measurements were conducted every 5 days during the storage period.Weight loss, decay rate, soluble solids content (SSC), acidity, pH, respiration rate, organic acids (malic, citric, ascorbic, fumaric, oxalic, succinic and tartaric) and phenolic compounds (catechin, chlorogenic, ferulic, gallic, o-coumaric, p-coumaric, protocatechuic, quercetin, rutin and syringic) were analyzed as quality parameters during storage. Results In the study, in general, the best values were observed in 6% CaCl2-treated fruits in terms of weight loss, SSC, TA, decay and respiration rates, although they varied according to different storage periods. Similarly, in terms of phenolic compounds, organic acids and vitamin C, 6% CaCl2-treated fruits had significantly better prevention of quality losses. In addition, the most common phenolic compound of strawberry fruits was gallic acid, followed by chlorogenic acid and catechin, respectively. On the other hand, the predominant organic acid observed in the fruits was malic acid, followed by citric acid, succinic acid and oxalic acid, respectively. Conclusions In this study, it was observed that CaCl2 applications more effectively prevented weight loss and decay rate by reducing the respiration rate compared to the control group at the end of the storage period (15th day). It was concluded that, particularly, the 6% CaCl2 dose can be used as an important treatment to extend storage life by preserving fruit quality and biochemical changes.

Objective: The need for new biomaterials with biocompatibility, mechanical, and antimicrobial properties continues in regenerative endodontic clinical applications in dentistry. Biodentine™ is successfully used in vital pulp therapies and has regenerative effects. Additionally, graphene, which possesses good physicochemical, mechanical, and biological properties, promotes cellular biomineralization and osteogenic differentiation in dentistry, along with its neuroregenerative effect. This study aims to investigate the effects of graphene and Biodentine™ on cell proliferation, oxidative stress, and odontogenic differentiation in human dental pulp stem cells (hDPSCs).Methods: Cryopreserved hDPSCs purchased from American Type Culture Collection (ATCC) were used in our study (Cat No: PT-5025). hDPSCs were seeded into the E-plate wells and subsequently four different doses of graphene (12.5, 25, 50 and 100 µg/ml) and Biodentine™ (2, 4, 8 and 16 µg/ml) were added. Results of MTT, total antioxidant capacity (TAC), total oxidant status (TOS) and alkaline phosphatase (ALP) tests were obtained at the end of the 24th hr. 96 hr-real time cell index data were collected with xCELLigence® system. Resulting data were compared using one-way analysis of variance (ANOVA). Results: 12.5 µg/ml graphene and 2 µg/ml Biodentine™ were found to be the subgroups with the highest levels of cell proliferation and the lowest oxidative stress. Antioxidative effect was determined in all Biodentine™ doses but only in 12.5 µg/ml graphene. Odontogenic differentiation was observed in all doses of graphene and Biodentine™. Conclusion: 12.5 µg/ml graphene and 2 µg/ml Biodentine™ were observed to have positive impacts on the proliferation, oxidative stress and odontogenic differentiations of hDPSCs. Keywords: Cell proliferation. Graphene. Oxidative stress. Odontogenic differentiation. Tricalcium silicate