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The main aim of this study is to determine the chemical compounds ofOriganum compactumessential oils (OCEO) at three phenological stages (vegetative, flowering, and post-flowering) and to evaluate their antifungal activity against three dermatophytes fungal strains:Trichophyton violaceum, Trichophyton tonsurans, andTrichophyton mentagrophytesusing direct contact method. The main compounds of OCEO are carvacrol, thymol, p-cymene, and γ-terpinene. Oregano EOs showed important antifungal activities with some variability between EOs and fungal testing. At a concentration of 0.75% (v/v), the best inhibition values showed with OCEO at vegetative stage againstTrichophyton mentagrophytes(95.17%),Trichophyton tonsurans(92.47%), andTrichophyton violaceum(91.41%). The OCEO at vegetative stage also showed the best IC50 (concentration of an inhibitor where the response is reduced by half) values of 52.86, 0.56, and 0.57% (v/v) againstTrichophyton mentagrophytes,Trichophyton tonsurans, andTrichophyton violaceum, respectively. The findings reveal that OCEO is a good source of anti-dermatophytes agents.

Dans le présent travail, quatre échantillons de miel collectés des régions différentes sont analysés pour leur teneur en composés phénoliques et testés pour leur activité antioxydante. Les contenus en polyphénols totaux ont été déterminés par la méthode de Folin-Ciocalteu et les teneurs en flavonoïdes par la méthode de trichlorure d’aluminium (AlCl3). L’activité antioxydante a été évaluée par la méthode de balayage du radical 2,2-diphényl-1-picrylhydrazyl. L’estimation quantitative des flavonoïdes et des phénols totaux par la méthode colorimétrique a montré que le miel est riche en ces composés avec une différence significative entre les échantillons. Les teneurs en polyphénols s’étendent entre 124,60 ± 1,12 et 75,14 ± 0,78 mg EAG/g de miel ; alors que les pourcentages des flavonoïdes sont compris entre 43,24 ± 0,72 et 19,64 ± 0,83 mg EQ/g de miel. L’évaluation quantitative de l’effet antioxydant a montré que l’échantillon de Zoumi est le plus actif (IC50 = 48,15 μg/ml), cette valeur ne présente pas une différence significative avec les deux témoins positifs (acide ascorbique : IC50 = 27,20 μg/ml et Trolox : IC50 = 43,72 μg/ml). Nous avons trouvé une corrélation positive très élevée (R2 = 0,974) entre les teneurs en flavonoïdes et la capacité antioxydante, alors que cette corrélation est moins élevée (R2 = 0,821) entre l’activité antioxydante et les phénols totaux.

The main of this study is to evaluate the anticancer and anti-dermatophyte activities of Mentha pulegium, Rosmarinus officinalis and Lavandula stoechas essential oils. The cytotoxic effect ...

The aim of this study was the investigation of the in vitro antioxidant, antidiabetic, dermatoprotective, antidermatophyte, and antibacterial properties of Mentha viridis essential oil (MVEO). Chemical composition of MVEO was determined using GC-MS analysis. Antioxidant activity was estimated by three complementary methods: DPPH, FRAP and ABTS. The in vitro antidiabetic effect was evaluated by the inhibition of α-Amylase and α-Glucosidase. Inhibitory effects of tyrosinase and elastase were used to reveal the dermatoprotective property of MVEO. Antibacterial activity was tested against five strains using agar well diffusion assay and microtitration method. The chemical composition determination of MVEO revealed the presence of carvone (37.26), 1.8-Cineole (11.82), and Terpinen-4-ol (08.72%) as the main compounds. MVEO presented remarkable antioxidant effects by IC50 values of 80.45 ± 1.86 μg/mL, 101.78 ± 3.14 μg/mL, and 139.59 ± 3.12 μg/mL obtained by DPPH, FRAP and ABTS tests respectively. In vitro inhibition of enzymes implicated in hydrocarbures degradation showed that MVEO exhibited important inhibitory effects against α-Amylase (IC50 = 101.72 ± 1.86 μg/mL) and α-Glucosidase (IC50 = 86.93 ± 2.43 μg/mL). Moreover, MVEO demonstrated remarkable inhibition of tyrosinase (IC50 = 55.13 ± 1.01 μg/mL) and elastase (IC50 = 114.24 ± 1.22 μg/mL). The antifungal activity showed important inhibitions of three dermatophyte species (T. mentagrophytes, T. tonsurans and T. violaceum). The antibacterial activity showed an important bactericidal action against tested strains, especially, Staphylococcus aureus and Listeria monocytogenes (MIC = MBC = 0.25%). The findings of this work showed that volatile components of MVEO could constitute a new source for antioxidant, antidiabetic, dermatoprotective, antidermatophyte and antibacterial properties. However, further investigations regarding the isolation of MVEO main compounds and investigations of their biological properties are required.