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Your search keyword '"Fazeli Delshad, Banafsheh"' showing total 3 results
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3 results on '"Fazeli Delshad, Banafsheh"'

1. Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine

Author

Soltanpour Gharibdousti, Faezeh, Fazeli Delshad, Banafsheh, Falak, Reza, Shayanfar, Nasrin, Ganjalikhani Hakemi, Mazdak, Andalib, Alireza, and Kardar, Gholam Ali

Subjects
angiogenesis, lcsh:R, peptide based vaccine, Peptide-based vaccine, metastasis, lcsh:Medicine, Original Article, immunoinformatics, vegf
Abstract

Objective(s): Blocking of vascular endothelial growth factor (VEGF) plays a pivotal role in inhibition of metastasis and is a target for development of anti-angiogenic agents. In this study, a peptide-based vaccine was designed and its potential for induction of humoral immune responses, generation of neutralizing antibodies, inhibition of tumor growth and metastasis was determined.Materials and Methods: With online bioinformatics tools, a fragment of the VEGF-A was selected for a peptide-based vaccine. To enhance its antigenicity, the peptide was conjugated with Keyhole limpet hemocyanin and used to immunize mice. Then, the polyclonal anti-VEGF antibody titer was measured and its effect on proliferation of HUVEC cell line was investigated by MTT assay. Finally, we checked the effect of the peptide on tumor growth, metastasis, and survival rates in a mouse model of cancer.Results: The bioinformatics analysis of the selected region confirmed dis-similarity of the peptide with any other human protein and its acceptable antigenicity to stimulate a tumor-specific humoral response. Anti-VEGF antibody titers were significantly greater in vaccinated mice than in controls. IgG antibody from mice immunized with recombinant VEGF-A inhibited HUVEC proliferation (PConclusion: Production of high-titer antibody against the peptide vaccine indicated that the peptide has the potency to be used as a peptide-based vaccine for humoral inhibition of tumor growth and metastasis. The efficacy of the peptide should be further tested in primate models.

Published
2020

3. Evaluation of Serum Immunoreactivity to Common Indigenous Iranian Inhalation and Food Allergens in Patients with Meniere's Disease.

Author

Roomiani, Maryam, Dehghani Firouzabadi, Fatemeh, Delbandi, Ali-Akbar, Ghalehbaghi, Babak, Daneshi, Ahmad, Yazdani, Nasrin, Fazeli Delshad, Banafsheh, and Asghari, Alimohamad

Subjects
MENIERE'S disease, ALLERGENS, ENZYME-linked immunosorbent assay, IMMUNOGLOBULIN E, IRANIANS
Abstract

A few studies investigated the relationship between allergy and Meniere disease considering complete allergen panel. We aimed to evaluate the serum immunoreactivity in patients with Meniere's disease (MD) compared with healthy people according to common indigenous Iranian inhalation and food allergens. Thirty-nine patients with MD referred to Rasoul Akram Hospital (Tehran, Iran) were evaluated and compared with a 41 membered control group. A panel of common inhalation and food allergens (using an immunoblotting method), as well as total immunoglobulin E (IgE) level (using the sandwich enzyme-linked immunosorbent assay method), were checked on the patients' serum. The mean total IgE level was 193.85 ± 175.43 IU/ml in the patients with MD and 117.61 ± 138.05 IU/ml in the control group, which was significantly higher than the other subjects in the control group (P =.016). There was a significant difference between the two groups regarding inhalation allergens such as; sweet vernal grass, cultivated rye, cultivated oat, Russian thistle, goosefoot, and rough pigweed (P =.01–0.038). Patients with MD reported more reactive to food allergens such as; rye flour, hazelnut, pepper, citrus mix 2, potato, strawberry, and celery allergens. There was a significant relationship between Meniere and serum immunoreactivity to inhalation and food allergens (both P =.001). Conclusion: Serum total IgE level in patients with MD (in both inhalation and food allergens groups) was higher than the control group, and there was a relationship between MD and immunoreactivity to common indigenous inhalation and food allergens of Iran. [ABSTRACT FROM AUTHOR]

Published
2022
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