1. Dp71 Delta(78-79) dystrophin mutant stimulates neurite outgrowth in PC12 cells via upregulation and phosphorylation of HspB1
- Author
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Febe E. Cazáres‐Raga, Candelaria Merino-Jiménez, Solenne Chardonnet, Griselda Rodríguez‐Martínez, Alvaro Rendon, Jorge Aragón, Cédric Pionneau, Víctor Ceja, Cecilia Montañez, Departamento de Genetica Y Molecular Biology, Centro de Investigacion y de estudios Avanzados del I.P.N., Plateforme Post-génomique de la Pitié-Salpêtrière (P3S), UMS omique (OMIQUE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Université Pierre et Marie Curie - Paris 6 - UFR de Médecine Pierre et Marie Curie (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut de la Vision, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centro de Investigacion y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Consejo Nacional de Ciencia y Tecnologia (Conacyt) [233893, CB-2013-222054, ECOS-ANUIES-SEP-Conacyt M11-S02], and Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
0301 basic medicine ,Cell biology ,animal structures ,Neurite ,Cellular differentiation ,Neuronal Outgrowth ,Biology ,Dp71 Delta(78-79) ,Biochemistry ,Antibodies ,Dystrophin ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,Downregulation and upregulation ,Heat shock protein ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Nerve Growth Factor ,medicine ,Animals ,Electrophoresis, Gel, Two-Dimensional ,Phosphorylation ,Molecular Biology ,Heat-Shock Proteins ,Neurons ,Regulation of gene expression ,medicine.diagnostic_test ,Gene Expression Profiling ,Neurite outgrowth ,PC12 cells ,Cell Differentiation ,MS ,Molecular biology ,Neoplasm Proteins ,Rats ,HspB1 ,030104 developmental biology ,Gene Expression Regulation ,nervous system ,Mutation ,Signal transduction ,030217 neurology & neurosurgery ,Molecular Chaperones ,Signal Transduction - Abstract
International audience; PC12 cells acquire a neuronal phenotype in response to nerve growth factor (NGF). However, this phenotype is more efficiently achieved when the Dp71 Delta(78-79) dystrophin mutant is stably expressed in PC12-C11 cells. To investigate the effect of Dp71 Delta(78-79) overexpression on the protein profile of PC12-C11 cells, we compared the expression profiles of undifferentiated and NGF-differentiated PC12-C11 and PC12 cells by 2DE. In undifferentiated cultures, one protein was downregulated, and five were upregulated. Dp71 Delta(78-79) overexpression had a greater effect on differentiated cultures, with ten proteins downregulated and seven upregulated. The protein with the highest upregulation was HspB1. Changes in HspB1 expression were validated by Western blot and immunofluorescence analyses. Interestingly, the neurite outgrowth in PC12-C11 cells was affected by a polyclonal antibody against HspB1, and the level of HspB1 and HspB1Ser86 decreased, suggesting an important role for this protein in this cellular process. Our results show that Dp71 Delta(78-79) affects the expression level of some proteins and that the stimulated neurite outgrowth produced by this mutant is mainly through upregulation and phosphorylation of HspB1.
- Published
- 2016