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The primary objective of this study was to compare urban and peri-urban mammal assemblages and relate variation in these communities to local differences in vegetation. We surveyed 15 locations in both urban and peri-urban habitats (n = 30). Boundaries of our survey areas coincided with those of National Park Service (NPS) areas in central Virginia. Over a 14-month period, we used five trap-types to document species in three guilds. A total of 9 and 15 species were documented at urban and peri-urban locations, respectively. Top predators Canis latrans (Coyote) and Felis rufus (Bobcat) were undetected at urban sites, while mesopredators were consistently more abundant. The absence of four small prey species and reduced abundances of the most common native generalist, Peromyscus leucopus (White-footed Mouse), were also associated with urban locations. Multivariate analyses of relative abundance data indicated significantly dissimilar mammal communities in urban and peri-urban locations. Shrub cover...

Coyotes (Canis latrans) are novel predators throughout the southeastern United States and their depredation of white-tailed deer (Odocoileus virginianus) neonates may explain observed declines in some deer populations in the region, but direct evidence for such a relationship is lacking. Our objective was to quantify neonate survival rates and causes of mortality at the United States Department of Energy's Savannah River Site (SRS), South Carolina to directly evaluate degree of predation in this deer population. From 2006 to 2009, we radio-monitored 91 neonates captured with the aid of vaginal implant transmitters in pregnant adult females and opportunistic searches. Overall Kaplan-Meier survival rate to 16 weeks of age was 0.230 (95% CI ¼ 0.155-0.328), and it varied little among years. Our best-fitting model estimated survival at 0.220 (95% CI ¼ 0.144-0.320). This model included a quadratic time trend variable (lowest survival rate during the first week of life and increasing to near 1.000 around week 10), and Julian date of birth (survival probability declining as date of birth increased). Predation by coyotes was the most frequent cause of death among the 70 monitored neonates that died, definitively accounting for 37% of all mortalities and potentially accounting for as much as 80% when also including probable coyote predation. Predation by bobcats (Felis rufus) accounted for 7% (definitive) to 9% (including probable bobcat predation) of mortalities. The level of coyote-induced mortality we observed is consistent with the low recruitment rates exhibited in the SRS deer population since establishment of coyotes at the site. If representative of recruitment rates across South Carolina, current harvest levels appear unsustainable. This understanding is consistent with the recent declining trend in the statewide deer population. The effects of coyote predation on recruitment should be considered when setting harvest goals, regardless of whether local deer population size is currently above or below desired levels, because coyotes can substantially reduce fawn recruitment. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.

We investigated the role of water features as focal attractors for gray foxes (Urocyon ciner- eoargenteus), coyotes (Canis latrans), and bobcats (Felis rufus) in west Texas to determine if they were foci for interspecific interaction. Mixed effects models indicated that species partitioned use of water features spatially and temporally. Linear models indicated factors influencing relative activity at water features varied by species. For coyotes and bobcats, the water availability model, containing days since last rainfall and nearest-neighbor distance to water was best supported by the data, with relative activity increasing with time between rainfall and distance between waters. For gray foxes, the best approximating model indicated that relative activity was inversely correlated to coyote and bobcat activity indices, and positively correlated to topographical complexity. Encounters between carnivore species were low, with most occurring between coyotes and gray foxes, followed by coyotes and bobcats, and bobcats and gray foxes. These findings suggest a behavioral-environmental mechanism that may function to modulate resource partitioning by carnivores in the arid West. 2011 The Wildlife Society.

A review of medical records and necropsy reports from 1979-2003 found 40 neoplasms in 26 zoo felids, including five lions (Panthera leo, two males and three females), three leopards (Panthera pardus, two males and one female), one jaguar (Panthera onca, female), 11 tigers (Panthera tigris, three males and eight females), two snow leopards (Panthera uncia, one male and one female), two cougars (Felis concolor, one male and one female), one bobcat (Felis rufus, male), and one cheetah (Acinonyx jubatus, female). Animals that had not reached 3 yr of age or had been housed in the collection less than 3 yrs were not included in the study. Neoplasia rate at necropsy was 51% (24/47), and overall incidence of felid neoplasia during the study period was 25% (26/103). Neoplasia was identified as the cause of death or reason for euthanasia in 28% (13/47) of those necropsied. Neoplasms were observed in the integumentary-mammary (n=11), endocrine (n=10), reproductive (n=8), hematopoietic-lymphoreticular (n=5), digestive (n=3), and hepatobiliary (n=2) systems. One neoplasm was unclassified by system. Multiple neoplasms were observed in 11 animals. Both benign and malignant neoplasms were observed in all systems except for the hematopoietic-lymphoreticular systems where all processes were malignant. Of the endocrine neoplasms, those involving the thyroid and parathyroid glands predominated (n=8) over other endocrine organs and included adenomas and carcinomas. In the integumentary system, 63% (7/11) of neoplasms involved the mammary gland, with mammary carcinoma representing 83% (6/7) of the neoplasms. The rates of neoplasia at this institution, during the given time period, appears to be greater than rates found in the one other published survey of captive felids.

Domestic dogs (Canis familiaris) are frequent visitors to protected areas, but little is known about how they affect wildlife communities. We studied the effects of dogs on wildlife communities by comparing the activity levels of wildlife in areas that prohibited dogs with areas that allowed dogs. We measured wildlife activity on trails and up to 200 m away from trails using five methods: (1) pellet plots, (2) track plates, (3) remote triggered cameras, (4) on-trail scat surveys, and (5) mapping prairie dog (Cynomys ludovicianus) burrow locations. The presence of dogs along recreational trails correlated with altered patterns of habitat utilization by several species. Mule deer (Odocoileus hemionus) activity was significantly lower within 100 m of trails in areas that allowed dogs than in areas that prohibited dogs. Small mammals, including squirrels (Sciurus spp.) and rabbits (Sylvilagus spp.), also exhibited reduced levels of activity within 50 m of trails in areas that allowed dogs when compared with areas without. The density of prairie dog burrows was lower within 25 m of trails in areas that allowed dogs. The presence of dogs also affected carnivore activity. Bobcat (Felis rufus) detections were lower in areas that allowed dogs, and red fox (Vulpes vulpes) detections were higher. These findings have implications for the management of natural areas, particularly those that allow dogs to be off-leash.

Few researchers have developed large-scale habitat models for sympatric carnivore species. We created habitat models for red foxes ( Vulpes vulpes ), coyotes ( Canis latrans ) and bobcats ( Lynx rufus ) in southern Illinois, USA, using the Penrose distance statistic, remotely sensed landscape data, and sighting location data within a GIS. Our objectives were to quantify and spatially model potential habitat differences among species. Habitat variables were quantified for 1-km 2 buffered areas around mesocarnivore sighting locations. Following variable reduction procedures, five habitat variables (percentage of grassland patches, interspersion–juxtaposition of forest patches, mean fractal dimension of wetland patches and the landscape, and road density) were used for analysis. Only one variable differed ( P

Harvest records reveal that populations of bobcats (Lynx rufus) in New Hampshire have undergone substantial changes during the past 200 years. In the 1800s, a nearly continuous bounty program resulted in annual harvests that averaged ∼30 bobcats. Harvests increased in 1915, and fluctuated from 100 to 400 bobcats during the 1920s through the 1950s. In 1959, harvests peaked at 421 and then rapidly declined. By 1970, payment was made on only 10 bobcats, and legal status was changed from nuisance animal to game species in 1973. In 1989, trapping and hunting seasons were closed and bobcats were designated a protected species. After 15 years of protection, populations of bobcats seem to be remaining at modest levels. To understand what factors may have contributed to the remarkable rise and fall of bobcat populations, we compared the temporal distribution of harvests to comments by early naturalists, legislation to control bobcat abundance, and historical changes in land use. We then used two approaches with a geographic information system to identify the environmental features that may affect present-day populations. The empirical approach relied on a comparison of landscape characteristics associated with recent (1990–2004) observations of bobcats to characteristics found at a comparable set of random locations. We also examined the characteristics of townships that yielded the majority of historical bobcat harvests (1931–1965) and developed a process-oriented model to rank present-day habitat suitability. The irruption of bobcat populations coincided with the availability of early-successional habitats as abandoned agricultural lands reverted to second-growth forests during the first half of the 20th century. Likewise, bobcat populations rapidly declined as these forests matured and no longer supported abundant prey, especially New England cottontails (Sylvilagus transitionalis). Our efforts to identify habitat features associated with present-day populations had mixed results. The empirically derived model correctly classified only 52% of recent bobcat locations, whereas the process-oriented model indicated that nearly 88% of recent bobcat observations were associated with sites that were ranked at high suitability. The results of this study demonstrate the utility of information on historical harvests when addressing questions on the status of contemporary wildlife populations.

From May 1997 to July 1999, 24 gray foxes Urocyon cinereoargenteus were radio-marked and their fates monitored in a natural area of southern California to identify rates of survival and cause-specific mortality, and thus to assess population sustainability. Pup (0.4–1.0 years old) foxes had an 8-month (September–April) survival rate (0.34) that was lower than the 8-month (0.77) or 12-month (0.58) estimates for adult foxes. Interference competition was evident; 92% (11/12) of fox mortalities were the result of predation by sympatric coyotes Canis latrans or bobcats Felis rufus . Also, five of seven gray fox mortalities were outside of, or on the border of, the home range of the killed fox. Calculations indicated that the fox population would remain stable if survival of pups during their first 4 months of life was 0.68 ( vs 0.75 for adults during these months and 0.58 for older pups for 4 other months). This seems reasonable, yet sympatric carnivores, mainly coyotes, clearly influence the gray fox population in southern California.

We documented adult mortality and fawn recruitment of Sonoran pronghorn (Antilocapra americana sonoriensis) in Arizona. This population is endangered (N

International audience; Bartonella henselae is the main agent of cat scratch disease in humans and domestic cats are the main reservoir of this bacterium. We conducted a serosurvey to investigate the role of American wild felids as a potential reservoir of Bartonella species. A total of 479 samples (439 serum samples and 40 Nobuto strips) collected between 1984 and 1999 from pumas (Felis concolor) and 91 samples (58 serum samples and 33 Nobuto strips) collected from bobcats (Lynx rufus) in North America, Central America and South America were screened for B. henselae antibodies. The overall prevalence of B. henselae antibodies was respectively 19.4% in pumas and 23.1% in bobcats, with regional variations. In the USA, pumas from the southwestern states were more likely to be seropositive for B. henselae (prevalence ratio (PR) = 2.82, 95% confidence interval (CI) = 1.55, 5.11) than pumas from the Northwest and Mountain states. Similarly, adults were more likely to be B. henselae seropositive than juveniles and kittens (PR = 1.77, 95% CI = 1.07, 2.93). Adult pumas were more likely to have higher B. henselae antibody titers than juveniles and kittens (p = 0.026). B. henselae antibody prevalence was 22.4% (19/85) in bobcats from the USA and 33.3% (2/6) in the Mexican bobcats. In the USA, antibody prevalence varied depending on the geographical origin of the bobcats. In California, the highest prevalence was in bobcats from the coastal range (37.5%). These results suggest a potential role of wild felids in the epidemiological cycle of Bartonella henselae or closely related Bartonella species.

Two frozen, raw horse meat-based diets fed to captive exotic felids at Brookfield Zoo were irradiated to determine the extent of microbial destruction and whether radiation treatment would affect consumption and/or fecal consistency in exotic cats. Fifteen cats, two African lions (Panthera leo), two Amur tigers (Panthera tigris altaica), one Amur leopard (Panthera pardus orientalis), two clouded leopards (Neofelis nebulosa), two caracals (Felis caracal), one bobcat (Felis rufus), and five fishing cats (Felis viverrinus), housed at Brookfield Zoo were fed nonirradiated and irradiated raw diets containing horse meat with cereal products and fortified with nutrients: Nebraska Brand Feline and/or Canine Diet (Animal Spectrum, North Platte, Nebraska 69103, USA). Baseline data were obtained during a 2-wk control period (nonirradiated diets), which was followed by a 4-wk period of feeding comparable irradiated diets. Feed intake and fecal consistency data were collected. An estimated radiation dose range of 0.5-3.9 kilograys reduced most microbial populations, depending on specific diet and microbe type. Irradiation had no overall effect on either feed consumption or fecal consistency in captive exotic cats, regardless of species, age, sex, or body mass. Data indicate that irradiation of frozen horse meat-based diets (packaged in 2.2-kg portions) result in microbial destruction in these products but that product storage time between irradiation and sampling may also affect microbial reduction. However, irradiation would be an appropriate method for reducing potentially pathologic bacteria in raw meat fed to exotic cats.

During a survey of carnivores and omnivores for bovine tuberculosis conducted in Michigan (USA) since 1996, Mycobacterium bovis was cultured from lymph nodes pooled from six coyotes (Canis latrans) (four adult female, two adult male), two adult male raccoons (Procyon lotor), one adult male red fox (Vulpes vulpes), and one 1.5-yr-old male black bear (Ursus americanus). One adult, male bobcat (Felis rufus) with histologic lesions suggestive of tuberculosis was negative on culture but positive for organisms belonging to the Mycobacterium tuberculosis complex when tested by polymerase chain reaction. All the tuberculous animals were taken from three adjoining counties where M. bovis is known to be endemic in the free-ranging white-tailed deer (Odocoileus virginianus) population. There were two coyotes, one raccoon, one red fox, and one bobcat infected in Alpena county. Montmorency County had two coyotes and one raccoon with M. bovis. Two coyotes and a bear were infected from Alcona County. These free-ranging carnivores/omnivores probably became infected with M. bovis through consumption of tuberculous deer. Other species included in the survey were opossum (Didelphis virginiana), gray fox (Urocyon cinereoargenteus), and badger (Taxidea taxus); these were negative for M. bovis.

We examined the relative roles of dominance in agonistic interactions and energetic constraints related to body size in determining local abundances of coyotes (Canis latrans, 8-20 kg), gray foxes (Urocyon cinereoargenteus, 3-5 kg) and bobcats (Felis rufus, 5-15 kg) at three study sites (hereafter referred to as NP, CP, and SP) in the Santa Monica Mountains of California. We hypothesized that the largest and behaviorally dominant species, the coyote, would exploit a wider range of resources (i.e., a higher number of habitat and/or food types) and, consequently, would occur in higher density than the other two carnivores. We evaluated our hypotheses by quantifying their diets, food overlap, habitat-specific abundances, as well as their overall relative abundance at the three study sites. We identified behavioral dominance of coyotes over foxes and bobcats in Santa Monica because 7 of 12 recorded gray fox deaths and 2 of 5 recorded bobcat deaths were due to coyote predation, and no coyotes died as a result of their interactions with bobcats or foxes. Coyotes and bobcats were present in a variety of habitats types (8 out of 9), including both open and brushy habitats, whereas gray foxes were chiefly restricted to brushy habitats. There was a negative relationship between the abundances of coyotes and gray foxes (P=0.020) across habitats, suggesting that foxes avoided habitats of high coyote predation risk. Coyote abundance was low in NP, high in CP, and intermediate in SP. Bobcat abundance changed little across study sites, and gray foxes were very abundant in NP, absent in CP, and scarce in SP; this suggests a negative relationship between coyote and fox abundances across study sites, as well. Bobcats were solely carnivorous, relying on small mammals (lagomorphs and rodents) throughout the year and at all three sites. Coyotes and gray foxes also relied on small mammals year-round at all sites, though they also ate significant amounts of fruit. Though there were strong overall interspecific differences in food habits of carnivores (P

Published reports of survival and cause-specific mortality for pronghorn (Antilocapra americana) indicate that mortality differs regionally and seasonally with sex, age and density of pronghorn. Cold winters with deep crusted snow is a primary source of mortality on adult pronghorn, however, predation also can be an important source of adult mortality. We report a visual observation of bobcat (Felis rufus) predation on an adult female pronghorn in northwestern South Dakota.

Minisatellite DNA profiles using the multilocus human probe 33.6 are presented for 27 captive bobcats (Felis rufus) of documented geographic and genetic origins. The results show that 30% of the finger-printing bands present in males are sex linked. The effect of sex on band sharing was attributed to the presence of male-specific hemizygotic bands belonging to a minisatellite cluster located in the nonrecombinant region of the Y chromosome. A combination of mechanisms might drive the dynamics of minisatellite loci and allow different evolutionary rates depending on the recombinational capability of the chromosomal locations involved. We discuss the utility of sex-linked fingerprinting bands as genetic markers for the study and management of bobcats.

Lung tissue from 52 bobcats, Lynx rufus, from Alabama, Kansas, New Mexico, Oklahoma, and Virginia were examined for the presence of lung-inhabiting parasites by gross inspection and histological examination. Parasites identified included Metathelazia californica, Troglostrongylus wilsoni, Vogeloides felis, and Paragonimus kellicotti. No parasite was discovered in bobcat lungs from New Mexico or Virginia. Distributions of M. californica and V. felis infections have been extended to include bobcats from Alabama, Kansas, and Oklahoma. The distribution of P. kellicotti has been extended to include bobcats from Alabama and Oklahoma, whereas that of T. wilsoni now includes bobcats from Alabama.

Reliable estimates of status and population trend are critical for conservation of large terrestrial carnivores, but are usually lacking due to the high costs of sampling across large geographic areas. For detecting population trends of mountain lion Felis concolor californica, we evaluated counts of track sets on 48 randomly chosen quadrats in California. Each quadrat contained 33.8 km of transect on dusty, dirt roads, which were chosen by local wildlife biologists. A count of track sets by one person on all quadrats was more efficient than recording presence/absence by local survey teams. We estimated an efficient sample size of 44 quadrats in California after applying our data to a general formula for contagious distributions. This sample size can be reduced substantially by choosing new transect locations based on associations of tracks with topography and habitat. Tracks were most likely found on roads along 1st- and 2nd-order streams, on mountain slopes and knolls/peaks and in oak woodland and montane hardwood conifer forest. A changing mountain lion population can be detected with an inexpensive, periodic track survey and self-stratifying, non-parametric tests. Each track survey across California can be finished in 30 days. The many mountain lions and the variety of environmental conditions included at this extraordinarily large spatial scale permit estimates of: (1) trends among population strata in quadrats that are clustered according to typical number and age/sex class of track sets; (2) population size and demography after individuals are identified by their tracks, and after linear density on roads is calibrated from spatial density at intensive study, sites: and (3) spatio-temporal associations with bobcat Felis rufus, black bear Ursus americanus, coyote Canis latrans, and fox Vulpes vulpes and Urocyon cinereoargenteus.

Many felids are threatened by loss of habitat, lack of genetic diversity, and over-exploitation. The reintroduction of bobcats (Felis rufus) to Cumberland Island, Georgia provided an opportunity to reintroduce a mid-sized felid without the concern for species survival that is paramount with endangered species. We captured bobcats from the coastal plain region of Georgia, briefly held them in captivity, and released them on Cumberland Island. We describe and evaluate the protocols and techniques used to accomplish the reintroduction. Future reintroductions of felids should consider the problem of post-release dispersal, although our island was relatively isolated and inhibited dispersal. Also, any reintroduction effort should invest effort and resources into post-release monitoring of the population. Empirical knowledge about the effects of spatial distribution, genetics, population dynamics, especially mechanisms of population regulation, behavior, and environmental conditions on the viability of populations is critical to the conservation of endangered species. Future research of the bobcats on Cumberland Island will be able to address aspects of the population and genetic dynamics of a small, insular felid population.

Unlike other regions of North America, forested habitats in New England bave increased substantially in the past 100 years. The proportion of land in New Hampshire covered by forests was 47% in 1880 and 87% in 1980. This increase was largely the result of a region-wide abandonment of farms and the subsequent colonization of these lands by second-growth forests. I examined the sequence of farm abandonment, forest colonization and forest maturation that occurred in New Hampshire in relation to changes in the abundance and distribution of a group of forest mammals and birds that have undergone substantial declines. A modeled pattern of secondary succession resulted in the availability of approximately 195,000 ha of early seral habitats (10–25 years after abandonment) from 1905 to 1940. These habitats then mutured into closed-canopy forests by about 1960. Concurrent to the loss of early successional habitats, populations of New England cottontails (Sylvilagus transitionalis) decreased from an apparent continuous distribution throughout 60% of New Hampshire to fragmented populations that occupy less than 20% of the state Bobacts (Felis rufus) responded functionally (S. transitionalis in diet: 1951–1954 = 43%, 1961–1964 = 10%) and numerically (mean annual harvest of bobcats: 1951–1954 = 350, 1965–1969 = 36) to changes in cottontail abundance. Eighteen of 26 species of migratory passerines that nest in the forests of northern New England also declined during the period their populations were monitored (1934–1987). Eight (44%) of the species that declined are associated with early successional habitats, and these species consistently exhibited population declines during the 1950s. The reduction of early successional species may be extended in space and time by current land uses that fragment and isolate patches of habitat. Ownership patterns of forest lands in New England (excluding Maine) reveal 88% private ownership with an average holding of 10 ha. This suggests that large tracts of early successional habitats will be restricted to industrial and state/national forests. Although even-aged management of a portion of these forests may be prerceived as incompatible with area-sensitive and interior species, clustering of clearcuts and maintaining large tracts of mature habitats could sustain diverse populations of forest vertebrates. Resumen: A diferencia de otras regiones en Norte America, los habitats boscosos en Nueva Inglattera se han incrementado substancialmente en los ultimos 100 anos. La proporcion de tierra cubierta por bosques en New Hampshire fue del 47 % en 1880 y del 87% en 1980. Este incremento fue, en gran medida, el resultado de un abandono a nivel regional de granjas y la subsecuente colonizacion de estas tierras por un crecimiento secundario de bosques. Yo examine la secuencia del abandono de las granjas, la colonizacion de los bosques y la maduracion de los bosques que ha ocurrido en New Hampshire en relacion con los cambios en la abundancia y distribucion de un grupo de mamiferos y pajaros que han sufrido declinaciones substanciales. Un patron modelado de sucesion secundaria resulto en la disponibilidad de aproximadamente 195,000 ha de habitats serales tempranos (10–25 anos despues del abandono) entre 1905 y 1940. Subsecuentemente, hacia 1960, estos habitats, maduraron hacia bosques de canopeo cerrado. Conjuntamente con la pardida de habitats sucesionales tempranos, las poblaciones de conejos (Sylvilagus transitionalis) de Nueva Inglaterra decrecieron de una distribucion aparentemente continua a traves del 60% de New Hampshire, a una poblacion fragmentada que ocupa < 20% del estado. Los “bobcats” (Felis rufus) respondieron funcionalmente (S. transitionalis en dieta; 1951–54 = 43% versus 1961–64 = 10%) y numericamente (media anual de la captura de Linces: 1951–54 = 350, 1965–69 = 36) a cambios en la abundancia de conejos. Dieciocho de las 26 especies de paserinidos migratorios que anidan en los bosques del norte de Nueva Inglaterra tambien declinaron durante el periodo en que la poblacion fue monitoreada (1934–87). Ocho (44%) de las especies que declinaron estan asociadas con habitats sucesionales tempranos, y estas especies consistentemente exhibieron declinaciones durante los anos 50. La reduccion de especies sucesionales tempranas puede ser extendida (en espacio y tiempo) por los usos actuales de la tierra que fragmentan y aislan los patches de habitat. Los patrones de tenencia de tierra de bosques en Nueva Inglaterra (excluyendo Maine, 88% en manos privadas con una media de tenencia de 10 ha) sugieren que grandes extensiones de habitats sucesionales tempranos van a estar restringidos a usos industriales y reservas estatales/nacionales. Aunque un manejo con stands de la misma edad de porciones de estos bosques puede ser percibido como incompatible con especies sensibles al area y con especies interiores, el agrupamiento de areas taladas y el mantenimiento de grandes extensiones de habitats maduros pueden sustentar poblaciones diversas de vertebrados del bosque.

We studied the hematology and blood chemistry of 33 adult bobcats (Felis rufus) captured from 1982 through 1985 in southeastern Idaho during a decline in lagomorphs, their major prey. Our objectives were to relate blood physiology of bobcats to sex, season, and a decline in abundance of black-tailed jackrabbits (Lepus californicus) and Nuttall's cottontail rabbits (Sylvilagus nuttallii). Males had higher (P

Intraspecific and intrasexual variation in home range size, body mass and ecological productivity is examined in three selected species of Carnivora (Felis rufus: Canis latrans; Ursus americanus), reflecting different diets. We then compare the intraspecific results with prior cross-species studies. Home range size and body mass inFelis rufus are positively and significantly correlated, similar to other comparative studies. For both intraspecific and intrasexual analyses of all three species, home range size and latitude are strongly correlated. Home range size scales with latitude more steeply as the proportion of meat increases in the diet of each species. That is, the slope of home range size on latitude is greatest in the meat-eatingFelis rufus followed by a shallower slope in the omnivorousCanis latrans and the most shallow slope in the frugivorous/folivorousUrsus americanus. These differences in slope are consistent with models of trophic level biomass change with variation in productivity, and parallel the dietary differences observed in cross-species differences in home range.

A bobcat (Felis rufus) estimated to be 6-mo old exhibited head pressing, stupor, intermittent seizures, and vocalization. Based on gross and histopathologic features, it was diagnosed to have severe focally extensive protozoal meningoencephalitis. Toxoplasma gondii was confirmed as the etiologic agent by an avidin-biotin immunohistochemical test. This is the first report of clinical toxoplasmosis in a free-ranging bobcat.

Thirty feral cats (Felis catus) from Alachua county (northern Florida) and 30 from Palm Beach county (southern Florida) were examined for hookworms. Two species, Ancylostoma tubaeforme and Ancylostoma braziliense, were identified. Forty-five cats (75%) were infected with A. tubaeforme, with a mean intensity of 48 hookworms per cat. Twenty cats (33%) were infected with A. braziliense, with a mean intensity of 28 worms per cat. The prevalence of A. tubaeforme was greater than that of A. braziliense in Alachua (P=0.002) and Palm Beach (P=0.004) counties. The intensity of A. tubaeforme infections was higher in Palm Beach county than Alachua county (P=0.013). The intensities of A. tubaeforme and A. braziliense were positively correlated (increased together) in Palm Beach county (P=0.011). These hookworms have also been identified in bobcats (Felis rufus), gray foxes (Urocyon cinereoargenteus), and red foxes (Vulpes vulpes) in Florida. The prevalence of A. tubaeforme was significantly greater in feral cats than those reported in bobcats (P

An 11-mo-old captive-bred male neutered bobcat (Felis rufus) presented with lethargy, anorexia, leukopenia, neutropenia, lymphopenia, and nonregenerative anemia. The animal was diagnosed as feline leukemia virus (FeLV) positive by immunofluorescent antibody and enzyme-linked immunosorbant assay (ELISA) testing. It died despite supportive care. Pathologic examination revealed multifocal non-suppurative encephalitis, diffuse interstitial pneumonia, multifocal hepatocellular necrosis, non-suppurative peritonitis, and lymphoid depletion. FeLV was isolated from peripheral blood mononuclear cells, bone marrow, spleen, and lymph node. FeLV-specific gag sequences were amplified by DNA polymerase chain reaction (PCR) and aligned with known domestic cat FeLV's. The source of the virus was speculated to be a domestic cat that was a surrogate nurse. Case reports of FeLV in nondomestic felids are few, and FeLV does not appear to be enzootic in wild felids, except European wildcats (Felis silvestris) in France and Scotland. Introduction of FeLV into free-living and captive nondomestic felid populations could have serious consequences for their health and survival. Measures to prevent the introduction of this virus to nondomestic felids are warranted.

Papillomaviruses (PVs) are highly species- and site-specific pathogens of stratified squamous epithelium. Although PV infections in the various Felidae are rarely reported, we identified productive infections in six cat species. PV-induced proliferative skin or mucous membrane lesions were confirmed by immunohistochemical screening for papillomavirus-specific capsid antigens. Seven monoclonal antibodies, each of which reacts with an immunodominant antigenic determinant of the bovine papillomavirus L1 gene product, revealed that feline PV capsid epitopes were conserved to various degrees. This battery of monoclonal antibodies established differential expression patterns among cutaneous and oral PVs of snow leopards and domestic cats, suggesting that they represent distinct viruses. Clinically, the lesions in all species and anatomic sites were locally extensive and frequently multiple. Histologically, the areas of epidermal hyperplasia were flat with a similarity to benign tumors induced by cutaneotropic, carcinogenic PVs in immunosuppressed human patients. Limited restriction endonuclease analyses of viral genomic DNA confirmed the variability among three viral genomes recovered from available frozen tissue. Because most previous PV isolates have been species specific, these studies suggest that at least eight different cat papillomaviruses infect the oral cavity (tentative designations: Asian lion, Panthera leo, P1PV; snow leopard, Panthera uncia, PuPV-1; bobcat, Felis rufus, FrPV; Florida panther, Felis concolor, FcPV; clouded leopard, Neofelis nebulosa, NnPV; and domestic cat, Felis domesticus, FdPV-2) or skin (domestic cat, F. domesticus, FdPV-1; and snow leopard, P. uncia, PuPV-2).

During a survey for tuberculosis in wild carnivores and omnivores, Mycobacterium bovis was cultured from pooled lymph nodes of three adult female coyotes (Canis latrans) harvested by hunters in Michigan (USA). No gross or histologic lesions suggestive of tuberculosis were seen in these animals. One coyote was taken from Montmorency county and two coyotes from Alcona county located in the north-eastern portion of Michigan's Lower Peninsula where free-ranging white-tailed deer (Odocoileus virginianus) have been found infected with bovine tuberculosis. It is thought that these coyotes became infected with M. bovis through the consumption of tuberculous deer. Other species included in the survey were the opossum (Didelphis virginiana), raccoon (Procyon lotor), red fox (Vulpes vulpes), bobcat (Felis rufus), and badger (Taxidea taxus).

In order to determine the importance of wild felids in the epidemiology of Bartonella spp. infection, 136 Nobuto strips or serum samples from free-ranging mountain lions (Felis concolor) and bobcats (Felis rufus) captured in California (USA) between 1985 and 1996 were tested for B. henselae antibodies (titeror = 1:64) using an immunofluorescence test. Similarly, 124 serum samples from 114 captive wild cats representing 26 species or subspecies collected between 1991 and 1995 were retrieved from the serum banks of four California zoological parks. Fifty-three percent (33/62) of the bobcats, 35% (26/74) of the mountain lions, and 30% (34/114) of the captive wild felids (genera Acinonyx, Panthera and Felis) had B. henselae antibodies. In captive wild felids, prevalence varied widely among the species, but seropositivity was more likely to occur in the genus Felis than in the genus Acinonyx or Panthera. Prevalence was evenly distributed between sexes, except for free-ranging mountain lions. Antibody prevalence ranged from 25% in 0- to 2-yr-old captive felids to 35% in catsor = 9-yr-old, but the highest antibody titers were observed in cats5-yr-old.

Notoedric mange was diagnosed in a free-ranging adult male ocelot (Felis pardalis) found dead in April 1994 in southern Texas (USA). The emaciated carcass had no body fat. The heart was enlarged and flaccid. There was a nonpurulent serosanguineous pericardial and peritoneal exudate. Severe encrusted skin lesions and alopecia extended from the head posteriorly to the shoulders. The forelegs and feet were less severely affected. We observed a massive infection of Notoedres cati in skin scrapings. Epidermal lesions included hyperkeratosis and parakeratosis with necrotic debris and foci of acute inflammatory cells surrounding mites and their eggs in the stratum corneum. There was mild acanthosis and spongiosis of the stratum germativum, but no chronic inflammation in the dermis. This is the first confirmed case in the ocelot, but notoedric manage has been reported from the bobcat (Felis rufus) in southern Texas. Thus, notoedric mange could pose an additional threat to the already endangered remnant population of the ocelot.

Ixodid ticks were present in all 189 samples examined from 53 Florida panthers (Felis concolor coryi, 104 collections) and 85 bobcats (Felis rufus floridana) in Florida (USA) between 1974 and 1991. We identified 3,251 ticks from panthers and 918 from bobcats. Specimens of Dermacentor variabilis, Ixodes scapularis, I. affinis, Amblyomma maculatum, and A. americanum were present on 49, 39, 17, seven, and two of the 53 Florida panthers, respectively, and comprised 36%, 55%, 7%, 1%, and

We immobilized 10 ocelots (Felis pardalis), and 21 bobcats (F. rufus) in south Texas (USA) during March to November 1991 with a mixture of ketamine hydrochloride (KH) and xylazine hydrochloride (XH); two ocelots were immobilized twice. Species were immobilized with (mean +/- SE) 14.7 +/- 1.6 mg KH/kg body mass for ocelots, 13.3 +/- 1.8 mg KH/kg for bobcats, and 1.1 +/- 0.1 mg XH/kg and 1.2 +/- 0.1 mg XH/kg for ocelots and bobcats, respectively. Immobilization times in bobcats were longer (P = 0.08) than in ocelots. Adult female ocelots (18.5 +/- 2.6 mg/kg) needed larger (P0.05) doses of KH than adult males (12.0 +/- 1.7 mg/kg). Bobcats were immobilized during summer with lower initial (8.6 +/- 0.9 mg/kg, P0.001) and total (10.1 +/- 1.3 mg/kg, P = 0.02) doses of KH than bobcats immobilized in winter (14.5 +/- 1.0 mg/kg, and 18.5 +/- 3.8 mg/kg, respectively); summer immobilization times (44.3 +/- 3.8 min) were also shorter (P = 0.03) than during winter (59.1 +/- 5.2 min). Bobcats immobilized during summer had lower (P0.01) initial rectal temperatures (39.4 +/- 0.2 C) than bobcats trapped in winter (41.1 +/- 0.4 C). Overall, we observed no effects of KH-XH dose on body temperature.

The Roosevelt Community Development Study (RCD) involved the testing and excavation of 27 sites in the Lower Tonto Basin of central Arizona. This is one of three related data recovery projects undertaken in the Tonto Basin for the Bureau of Reclamation prior to the raising of the Roosevelt Lake dam. The results of the RCD project are presented in four Anthropological Papers of the Center for Desert Archaeology: Anthropological Papers No. 12 is the research design; Anthropological Papers No. 13 (two volumes) contains background information and the site descriptions; Anthropological Papers No. 14 (three volumes) includes the artifact and environmental analyses; and Anthropological Papers No. 15 presents the synthesis and conclusions. The project was situated within the Tonto National Forest and covered a four-mile, continuous area along the north bank of the Salt River. Sites within the project area exhibited a great range of functional, temporal, and, possibly, cultural diversity. These included two sites with platform mounds (the Meddler Point and Pyramid Point sites); a 10Q-room masonry pueblo (the Griffin Wash site); smaller masonry compounds (e.g., the Porcupine site); and pithouse hamlets and farmsteads (e.g., the Hedge Apple and Eagle Ridge sites). Temporal components ranged from the Early Ceramic period (A.D. 100-600), at Locus B of the Eagle Ridge site, through the Roosevelt phase (A.D. 1250-1350) of the Classic period. The Early Ceramic component of the Eagle Ridge site is now the earliest documented ceramic period site in the Tonto Basin and provides definitive evidence for an indigenous ceramic-using population. The project area was inhabited most intensively during the Roosevelt phase, when platform mounds, pueblo room blocks, and small masonry compounds were occupied. Architectural and artifact variability suggest the presence of several different cultural groups co-residing in the Tonto Basin at this time, and migration is believed to have been a significant process in Tonto Basin prehistory. The RCD project area was largely abandoned by A.D. 1325, prior to the large-scale aggregation that occurred during the Gila phase; very few Gila Polychrome sherds were recovered from project area sites. The mandate of the RCD project, as spedfied by the Bureau of Reclamation, was to investigate the temporal and developmental sequence of the prehistoric populations within this area. To meet these goals, six sites were intensively examined through full-scale excavation, and an extensive data set was gathered from the remaining 21 sites. The three volumes in Anthropological Papers No. 14 contain the artifact and environmental analyses. More than 150,000 artifacts were recovered from the RCD excavations. This volume (Volume 3) presents the paleobotanical and osteological data. Included are analyses of the pollen (Chapter 18), flotation (Chapter 19), faunal (Chapter 20), and mortuary (Chapter 21) assemblages. Data from the subsistence analyses are combined and synthesized in Chapter 22. Volume 1 of Anthropological Papers No. 14 presents the analyses of the chipped stone, ground stone, jewelry and personal ornament, and shell assemblages. The ceramic artifact assemblage is examined in Volume 2 More spedfic information on the individual sites and the project background can be found in Anthropological Papers No. 13. Anthropological Papers No. 15 integrates and synthesizes these data to provide a more inclusive view of the prehistoric occupation of the RCD project area and the Tonto Basin.

Sarcocysts were found in the tongue, diaphragm, heart, intestinal tunica muscularis, and skeletal muscle of bobcats (Felis rufus floridanus) collected in Florida (USA). The tongue was found to be the best indicator tissue for sarcocysts (P less than 0.005). Thirty of 60 bobcats screened were found to contain sarcocysts in at least one of the muscle tissues examined. Of the positive bobcats, 28 of 28 tongues contained sarcocysts, while only 10 of 27 (37%), and 8 of 26 (31%) contained sarcocysts in the diaphragm or cardiac muscle, respectively. Although immune suppression has been suggested as a possible reason for formation of sarcocysts in some carnivores, no such correlation was evident in the bobcats. Comparisons of prey species taken by the panther and bobcat, and overlap of geographical range by the two species leave questions as to the source of infection, and the species of Sarcocystis that is infecting both felids.

A laboratory cat died 12 days after intraperitoneal inoculation of a 1 ml suspension containing 1.5 x 10(6) blood mononuclear cells from a Florida panther (Felis concolor coryi). Gross, histologic and ultrastructural investigations revealed the cause of death to be infection by Cytauxzoon felis, a protozoal parasite known to cause a rapidly fatal disease (cytauxzoonosis) in domestic cats. The bobcat (Felis rufus) has been identified as a natural host for C. felis. This report implicates the Florida panther as another possible host for C. felis.

Two of five bobcats (Felis rufus) from southwestern Arkansas had natural pulmonary infections of Paragonimus kellicotti and Filaroides rostratus. Pairs of P. kellicotti were found in spherical cyst-like structures approximately 1 cm in diameter. Filaroides rostratus were seen as serpentine pale white areas on the pleural surface and also firmly embedded in fibrous capsules in the pulmonary parenchyma. Histologic lesions associated with the presence of these parasites consisted primarily of a verminous bronchitis. Clinical signs or compromised pulmonary function were not associated with these infections.

A passive tracking index method that has been successfully applied to dingoes (Canis lupus dingo) in Australia was shown to have more general applicability to wild canids by monitoring coyote (Canis latrans) populations in southern Texas. The index was calculated simultaneously for multiple species of animals from observations on the number of intrusions onto a series of tracking plots over several days. We found that the index reflected changes in coyote activity before and after a trapping program on each of 2 ranches. We also were able to simultaneously monitor bobcat (Felis rufus) and white-tailed deer (Odocoileus virginianus) populations, producing some interesting (and unexpected) insights. In our study area, we found it difficult to distinguish the number of rabbit and rodent intrusions into the plots, but these animals might be indexed in other habitats. Analyses of the data as binary responses (presence or absence of spoor on each tracking plot), as has been done in scent-post surveys, reduced the sensitivity and accuracy of inferences.

Identification of mortality sources of white-tailed deer (Odocoileus virginianus), particularly predation and survival rates, is important for effective management. We captured, radiocollared, and monitored 78 white-tailed deer fawns in northcentral New Brunswick to determine survival and cause-specific mortality from February 1994 through May 1997. Of 50 fawns captured as neonates, 22 died by 30 November 1994-96. Predation by coyotes (Canis latrans; n = 9), black bears (Ursus americanus; n = 5), domestic dogs (Canis familiaris; n = 3), and bobeats (Felis rufus; n = 2) was the largest cause of fawn mortality during summer and autumn, Coyotes were the primary cause of mortality of fawns >7 months old (11 of 15). Fawn survival was lowest during summer (0.47). increased during autumn (0.86) and early winter (0.95), and then declined during late winter (0.76) and spring (0.81). Our results support the hypothesis that coyotes have replaced gray wolves (Canis lupus) in northeastern North America, with survival and mortality rates being comparable between New Brunswick and other areas where wolves and coyotes are sympatric.

Scent-station surveys have been widely used to monitor bobcat (Felis rufus) populations, but relationships between bobcat abundance and the index derived from scent-station surveys have not been validated. In autumn 1988 and 1989 we reintroduced bobcats (n = 31) to Cumberland Island, Georgia. We conducted 15 scent-station surveys during September-February 1988, 1989, and 1990 to obtain scent-station indices (SSI) as we increased bobcat density. We found a positive relationship (r 2 = 0.45, P = 0.0066) between population size and SSI. However, because SSI variance also was correlated positively with SSI, we transformed data to meet the assumption of homoscedasticity for the regression model (r 2 = 0.73, P < 0.001)

Cranial characters of bobcats (Felis rufus) from the eastern United States were examined using univariate and multivariate techniques to assess morphologic variation and to identify relationships among morphologic features and selected environmental variables. The patterns of variation produced using principal component analysis indicated that larger males occurred in northern and eastern localities, whereas N-central localities produced the largest females. The smallest individuals of both sexes occurred in the southern Appalachian Mountains. Comparisons of matrices for overall environmental variation and variation of individual environmental variables with matrices of morphologic variation showed a higher degree of correlation for females than for males. These results suggest that variation in female morphology may be under somewhat different selective constraints than corresponding variation in males.

I measured 11 habitat variables at bobcat (Felis rufus) diurnal loafing sites (n=22) and compared them to random sites (n=22) in southeastern Colorado during 1983-85. Univariate analysis of variables indicated bobcats selected steep-sloped, rocky areas with dense vertical cover and little herbaceous ground cover. Dense vertical cover (>70%) appeared to be the most significant feature of loafing sites, permitting correct classification of 88% of sites