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To connect human biology to fish biomedical models, we sequenced the genome of spotted gar (Lepisosteus oculatus), whose lineage diverged from teleosts before teleost genome duplication (TGD). The slowly evolving gar genome has conserved in content and size many entire chromosomes from bony vertebrate ancestors. Gar bridges teleosts to tetrapods by illuminating the evolution of immunity, mineralization and development (mediated, for example, by Hox, ParaHox and microRNA genes). Numerous conserved noncoding elements (CNEs; often cis regulatory) undetectable in direct human-teleost comparisons become apparent using gar: functional studies uncovered conserved roles for such cryptic CNEs, facilitating annotation of sequences identified in human genome-wide association studies. Transcriptomic analyses showed that the sums of expression domains and expression levels for duplicated teleost genes often approximate the patterns and levels of expression for gar genes, consistent with subfunctionalization. The gar genome provides a resource for understanding evolution after genome duplication, the origin of vertebrate genomes and the function of human regulatory sequences.

Abstract We show how effects of compositeness emerging in a Composite 2-Higgs Doublet Model can enter Standard Model (SM)-like Higgs boson pair production at the Large Hadron Collider in both resonant and non-resonant mode. Such effects can arise from modified trilinear Higgs self-couplings and top-Yukawa couplings, as well as from loops of new heavy quarks and additional quartic Higgs-fermion interactions. In the resonant case, significant distortions of the Breit-Wigner shape of a new scalar state decaying into the two SM-like Higgs states may occur due to interference effects amongst not only the SM-like diagrams but also those involving the new heavy quarks. In the non-resonant case, a modification of the underlying line-shape and a local maximum at twice a new heavy quark mass appear simultaneously. We quantify these effects by taking into account the relevant theoretical and latest experimental bounds.

Abstract Background By being highly involved in the tumor evolution and disease progression of small cell lung cancer (SCLC), Myc family members (C-Myc, L-Myc, and N-Myc) might represent promising targetable molecules. Our aim was to investigate the expression pattern and prognostic relevance of these oncogenic proteins in an international cohort of surgically resected SCLC tumors. Methods Clinicopathological data and surgically resected tissue specimens from 104 SCLC patients were collected from two collaborating European institutes. Tissue sections were stained by immunohistochemistry (IHC) for all three Myc family members and the recently introduced SCLC molecular subtype-markers (ASCL1, NEUROD1, POU2F3, and YAP1). Results IHC analysis showed C-Myc, L-Myc, and N-Myc positivity in 48%, 63%, and 9% of the specimens, respectively. N-Myc positivity significantly correlated with the POU2F3-defined molecular subtype (r = 0.6913, p = 0.0056). SCLC patients with C-Myc positive tumors exhibited significantly worse overall survival (OS) (20 vs. 44 months compared to those with C-Myc negative tumors, p = 0.0176). Ultimately, in a multivariate risk model adjusted for clinicopathological and treatment confounders, positive C-Myc expression was confirmed as an independent prognosticator of impaired OS (HR 1.811, CI 95% 1.054–3.113, p = 0.032). Conclusions Our study provides insights into the clinical aspects of Myc family members in surgically resected SCLC tumors. Notably, besides showing that positivity of Myc family members varies across the patients, we also reveal that C-Myc protein expression independently correlates with worse survival outcomes. Further studies are warranted to investigate the role of Myc family members as potential prognostic and predictive markers in this hard-to-treat disease.

Abstract The complex singlet extension CxSM of the Standard Model (SM) is a simple extension of the SM with two visible Higgs bosons in the spectrum and a Dark Matter (DM) candidate. In this paper we complete the computation of the next-to-leading (NLO) electroweak (EW) corrections to on-shell and non-loop-induced Higgs decays. Our calculations are implemented in the code EWsHDECAY which also includes the relevant QCD corrections. Performing an extensive parameter scan in the model and including all relevant theoretical and experimental single- and di-Higgs as well as DM constraints, we obtain a viable parameter sample. We find that current DM constraints are able to test the model in DM mass regions where collider searches are not sensitive. The relative EW corrections turn out to be large for scenarios with relatively large couplings, threshold effects or small leading-order (LO) widths. Otherwise, they are of typical EW size and can amount up to about 20–25%. The theory uncertainty derived from the change of the renormalization scheme dependence then is of a few per cent. While the NLO corrections applied in the constraints due to single- and di-Higgs searches impact the validity of specific parameter points, the overall shape of the allowed parameter region is not yet sensitive to the EW corrections. This picture will change with further increased experimental precision in the future and necessitates precise predictions on the theory side as presented in this paper.

Recent research has shown that social media services create large consumer surplus. Despite their positive impact on economic welfare, concerns are raised about the negative association between social media usage and well-being or performance. However, causal empirical evidence is still scarce. To address this research gap, we conduct a randomized controlled trial among students in which we track participants' daily digital activities over the course of three quarters of an academic year. In the experiment, we randomly allocate half of the sample to a treatment condition in which social media usage (Facebook, Instagram, and Snapchat) is restricted to a maximum of 10 minutes per day. We find that participants in the treatment group substitute social media for instant messaging and do not decrease their total time spent on digital devices. Contrary to findings from previous correlational studies, we do not find any significant impact of social media usage as it was defined in our study on well-being and academic success. Our results also suggest that antitrust authorities should consider instant messaging and social media services as direct competitors before approving acquisitions.

Brain-computer interfaces (BCIs) transfer human brain activities into computer commands and enable a communication channel without requiring movement.Among other BCI approaches, steady-state visual evoked potential (SSVEP)-based BCIs have the potential to become accurate, assistive technologies for persons with severe disabilities.Those systems require customization of different kinds of parameters (e.g. stimulation frequencies). Calibration usually requires selecting predefined parameters by experienced/trained personnel, though in real-life scenarios an interface allowing people with no experience in programming to set up the BCI would be desirable. Another occurring problem regarding BCI performance is BCI illiteracy (also called BCI deficiency). Many articles reported that BCI control could not be achieved by a non-negligible number of users. In order to bypass those problems we developed a SSVEP-BCI wizard, a system that automatically determines user-dependent key-parameters to customize SSVEP-based BCI systems. This wizard was tested and evaluated with 61 healthy subjects. All subjects were asked to spell the phrase ``RHINE WAAL UNIVERSITY'' with a spelling application after key parameters were determined by the wizard. Results show that all subjects were able to control the spelling application. A mean (SD) accuracy of 97.14 (3.73)% was reached (all subjects reached an accuracy above 85% and 25 subjects even reached 100% accuracy).

Chlorophyll fluorescence measurement is a widely used technique to determine photosynthetic performance. Light energy absorbed by a chlorophyll molecule can be dissipated by driving photochemical energy conversion, as heat in non-photochemical quenching processes, or it is re-emitted as fluorescence. The loss of light energy as chlorophyll fluorescence is primarily derived from photosystem II. Photosystem II is a thylakoid-embedded multiprotein complex which provides the high redox potential needed to oxidize water. Within photosystem II photons of light are captured and used to energize electrons. Energized electrons are fed into the linear electron transport chain and photosystem II replenishes lost electrons with electrons from splitting of water. Chlorophyll fluorescence yield can be quantified using a modulated fluorometer device. In such a device, a modulated measuring light beam (switched on and off at a high frequency) and the parallel detection of fluorescence exclusively excited by the measuring light allows chlorophyll fluorescence measurements in the presence of photosynthetic (actinic) light. In addition, high intensity, but short duration light flashes (saturating pulses) are used to determine maximum fluorescence yields in dark and light adapted leaves. In this protocol the procedure to receive a typical fluorescence graph of Arabidopsis wild-type leaves is given. Furthermore, this procedure can be used to identify Arabidopsis mutant plants affecting photosystem II, on the basis of the respective fluorescence graphs and values.

This paper presents a conceptual model of referee efficacy, defines the concept, proposes sources of referee specific efficacy information, and suggests consequences of having high or low referee efficacy. Referee efficacy is defined as the extent to which referees believe they have the capacity to perform successfully in their job. Referee efficacy beliefs are hypothesized to be influenced by mastery experiences, referee knowledge/education, support from significant others, physical/mental preparedness, environmental comfort, and perceived anxiety. In turn, referee efficacy beliefs are hypothesized to influence referee performance, referee stress, athlete rule violations, athlete satisfaction, and co-referee satisfaction.

This study aimed to problematize aspects of the work of professors in relation to gender, assessment policies, and health. For this purpose, a qualitative social investigation was conducted within the aspect of participatory studies and the views of materialist feminism. An analysis of the material was carried out through content analysis, according to the topic, using four main themes: conflicts between professors' work and domestic work; professors' work, motherhood, and guilt; policies for the assessment of professors and gender relations; and the sexual division of labor and teaching. It was possible to perceive just how much the demands of the productive sphere have gone beyond the time of the workday and into the reproductive sphere and the private life of professors, compromising the struggle for health and leading to processes of suffering and illness. The theme of the sexual division of labor in public universities appears to be an important issue that highlights work overload and psychological illness, especially at a time when the teaching profession is becoming increasingly competitive. In conclusion, we believe that there is an imperative need for investments in public policies that can guarantee gender equality in higher education.

The main objective of this article is to present and analyze the research instrument called "health and work notebooks", focusing on the production of knowledge concerning professors' work at a public university. The notebooks serve as a qualitative and participant research technique that is appropriate for the in-depth study of relations between health and work, viewing workplaces as privileged spaces for exercising effective action in the defense of health and the work experience as the principal material for analysis. The notebooks' special quality as a research technique lies in worker's role as protagonist in the research, as the diary's author and co-participant in the study. Eight professors participated, all from the same institute in a federal university (IFES) in Rio de Janeiro, Brazil. As for analysis of the empirical materials from the notebooks and consistent with the workers, the thematic analysis technique was adopted, producing four main discussion categories: time on the job and professors' multiple work activities; precarization of working conditions at universities; faculty health at limits; and the notebooks viewed from the authors' perspective. As for the results, the theme that stood out was work overload and time pressure to meet targets. Finally, the health and work notebooks proved to be a potential research tool for generating knowledge from a collective perspective.

The main objective of this article is to analyze the new organization of labor of university teachers, seeking to investigate the potential relationship with the health status of these workers. It is based on the assumption that job instability in public universities has had repercussions on the health of higher education teachers. A qualitative exploratory study was conducted by means of bibliographic research in indexed databases. As a method of analysis, thematic analysis was used, focusing on four empirical categories, namely: job instability in the teaching profession; intensification of labor; aspects of the organization of teaching work in universities; and data on the health of university teachers. It was revealed in the literature that the use of strong organizational pressures prevails in the university scenario and consequently the intensification of labor is prevalent, with emphasis on the issue of increasing the demand for academic productivity. It was also observed that the topic of excess workload of teachers is recurrent and the concept of availability of less leisure time prevails. In addition, the need for organized collective resistance was confirmed in order to modify the job instability of teaching work.