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1. Immunogenicity and reactogenicity of intradermal mRNA-1273 SARS-CoV-2 vaccination: a non-inferiority, randomized-controlled trial

Author

Prins, Manon L. M., Roozen, Geert V. T., Pothast, Cilia. R., Huisman, Wesley, van Binnendijk, Rob, den Hartog, Gerco, Kuiper, Vincent P., Prins, Corine, Janse, Jacqueline J., Lamers, Olivia. A. C., Koopman, Jan Pieter R., Kruithof, Annelieke C., Kamerling, Ingrid M. C., Dijkland, Romy C., de Kroon, Alicia. C., Azimi, Shohreh, Feltkamp, Mariet C. W., Kuijer, Marjan, Jochems, Simon P., Heemskerk, Mirjam H. M., Rosendaal, Frits R., Roestenberg, Meta, Visser, Leo G., and Roukens, Anna H. E.

Published
2024
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2. Immune responsiveness in stable kidney transplantation patients: Complete inhibition of T‐cell proliferation but residual T‐cell activity during maintenance immunosuppressive treatment.

Author

in 't Veld, Aliede E., Eveleens Maarse, Boukje C., Juachon, Maria J., Meziyerh, Soufian, de Vries, Aiko P. J., van Rijn, Aline L., Feltkamp, Mariet C. W., Moes, Dirk Jan A. R., Burggraaf, Jacobus, and Moerland, Matthijs

Subjects
KIDNEY transplantation, T cells, DEAD, DRUG monitoring, DRUG utilization, TREATMENT effectiveness, CELLULAR aging
Abstract

The recommended immunosuppressive treatment after kidney transplantation consists of tacrolimus, mycophenolate mofetil, and low‐dose corticosteroids. Drug concentrations are monitored using therapeutic drug monitoring (TDM), which does not necessarily correlate with pharmacodynamic activity. To find the balance between optimal efficacy and minimal toxicity, it might be more informative to monitor patients' immunological status rather than drug concentrations. We selected a panel of T‐cell‐based immune assays, which were used for immunomonitoring of 14 stable kidney transplantation patients. Whole blood was incubated with a T‐cell stimulus, after which T‐cell proliferation, T‐cell activation marker expression and cytokine production were measured to study residual immune activity in vitro (before drug intake; drug added to the incubation) and ex vivo (after drug intake). T‐cell proliferation was completely suppressed in all patients over the full day, while IL‐2, IFN‐γ, CD71, and CD154 showed fluctuations over the day with a strong inhibition (75%–25%) at 2 h post‐dose. The level of inhibition was variable between patients and could not be related to pharmacokinetic parameters or the presence of regulatory or senescence immune cells. Moreover, the level of inhibition did not correlate with the in vitro tacrolimus drug effect as studied by incubating pre‐dose blood samples with additional tacrolimus. Overall, IL‐2, IFN‐γ, CD71, and CD154 seem to be good markers to monitor residual immune activity of transplantation patients. To evaluate the correlation between these pharmacodynamic biomarkers and clinical outcome, prospective observational studies are needed. [ABSTRACT FROM AUTHOR]

Published
2024
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7. A taxonomy update for the family Polyomaviridae

Author

Calvignac-Spencer, Sébastien, Feltkamp, Mariet C. W., Daugherty, Matthew D., Moens, Ugo, Ramqvist, Torbjörn, Johne, Reimar, Ehlers, Bernhard, and Polyomaviridae Study Group of the International Committee on Taxonomy of Viruses

Published
2016
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9. Humoral response to SARS-CoV-2 infection among liver transplant recipients

Author

UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de gastro-entérologie, Becchetti, Chiara, Broekhoven, Annelotte G C, Dahlqvist, Géraldine, Fraga, Montserrat, Zambelli, Marco Fabrizio, Ciccarelli, Olga, Saouli, Anne-Catherine, Trizzino, Arianna, Banz, Vanessa, Dufour, Jean-François, Roukens, Anna H E, Torres Morales, Shessy P, Myeni, Sebenzile K, Kikkert, Marjolein, Feltkamp, Mariet C W, Coenraad, Minneke J, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de gastro-entérologie, Becchetti, Chiara, Broekhoven, Annelotte G C, Dahlqvist, Géraldine, Fraga, Montserrat, Zambelli, Marco Fabrizio, Ciccarelli, Olga, Saouli, Anne-Catherine, Trizzino, Arianna, Banz, Vanessa, Dufour, Jean-François, Roukens, Anna H E, Torres Morales, Shessy P, Myeni, Sebenzile K, Kikkert, Marjolein, Feltkamp, Mariet C W, and Coenraad, Minneke J

Abstract

Objective Immunosuppressive agents are known to interfere with T and/or B lymphocytes, which are required to mount an adequate serologic response. Therefore, we aim to investigate the antibody response to SARS-CoV- 2 in liver transplant (LT) recipients after COVID-19. Design Prospective multicentre case–control study, analysing antibodies against the nucleocapsid protein, spike (S) protein of SARS-CoV- 2 and their neutralising activity in LT recipients with confirmed SARS-CoV- 2 infection (COVID-19- LT) compared with immunocompetent patients (COVID-19- immunocompetent) and LT recipients without COVID-19 symptoms (non-COVID- 19- LT). Results Overall, 35 LT recipients were included in the COVID-19- LT cohort. 35 and 70 subjects fulfilling the matching criteria were assigned to the COVID-19- immunocompetent and non-COVID- 19- LT cohorts, respectively. We showed that LT recipients, despite immunosuppression and less symptoms, mounted a detectable antinucleocapsid antibody titre in 80% of the cases, although significantly lower compared with the COVID-19- immunocompetent cohort (3.73 vs 7.36 index level, p<0.001). When analysing anti-S antibody response, no difference in positivity rate was found between the COVID-19- LT and COVID-19- immunocompetent cohorts (97.1% vs 100%, p=0.314). Functional antibody testing showed neutralising activity in 82.9% of LT recipients (vs 100% in COVID-19- immunocompetent cohort, p=0.024). Conclusions Our findings suggest that the humoral response of LT recipients is only slightly lower than expected, compared with COVID-19 immunocompetent controls. Testing for anti-S antibodies alone can lead to an overestimation of the neutralising ability in LT recipients. Altogether, routine antibody testing against separate SARS-CoV- 2 antigens and functional testing show that the far majority of LT patients are capable of mounting an adequate antibody response with neutralising ability.

Published
2022

11. Humoral response to SARS-CoV-2 infection among liver transplant recipients

Author

Becchetti, Chiara, primary, Broekhoven, Annelotte G C, additional, Dahlqvist, Géraldine, additional, Fraga, Montserrat, additional, Zambelli, Marco Fabrizio, additional, Ciccarelli, Olga, additional, Saouli, Anne-Catherine, additional, Trizzino, Arianna, additional, Banz, Vanessa, additional, Dufour, Jean-François, additional, Roukens, Anna H E, additional, Torres Morales, Shessy P, additional, Myeni, Sebenzile K, additional, Kikkert, Marjolein, additional, Feltkamp, Mariet C W, additional, and Coenraad, Minneke J, additional

Published
2022
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14. Immune Determinants of Viral Clearance in Hospitalised COVID-19 Patients: Reduced Circulating Naïve CD4+ T Cell Counts Correspond with Delayed Viral Clearance.

Author

Zlei, Mihaela, Sidorov, Igor A., Joosten, Simone A., Heemskerk, Mirjam H. M., Myeni, Sebenzile K., Pothast, Cilia R., de Brouwer, Caroline S., Boomaars-van der Zanden, A. Linda, van Meijgaarden, Krista E., Morales, Shessy T., Wessels, Els, Janse, Jacqueline J., Goeman, Jelle J., Cobbaert, Christa M., Kroes, Aloys C. M., Cannegieter, Suzanne C., Roestenberg, Meta, Visser, Leonardus G., Kikkert, Marjolein, and Feltkamp, Mariet C. W.

Subjects
COVID-19, CD4 antigen, B cells, BASOPHILS, T cells, CRITICALLY ill, SARS-CoV-2, MONOCYTES
Abstract

Virus-specific cellular and humoral responses are major determinants for protection from critical illness after SARS-CoV-2 infection. However, the magnitude of the contribution of each of the components to viral clearance remains unclear. Here, we studied the timing of viral clearance in relation to 122 immune parameters in 102 hospitalised patients with moderate and severe COVID-19 in a longitudinal design. Delayed viral clearance was associated with more severe disease and was associated with higher levels of SARS-CoV-2-specific (neutralising) antibodies over time, increased numbers of neutrophils, monocytes, basophils, and a range of pro-inflammatory cyto-/chemokines illustrating ongoing, partially Th2 dominating, immune activation. In contrast, early viral clearance and less critical illness correlated with the peak of neutralising antibodies, higher levels of CD4 T cells, and in particular naïve CD4+ T cells, suggesting their role in early control of SARS-CoV-2 possibly by proving appropriate B cell help. Higher counts of naïve CD4+ T cells also correlated with lower levels of MIF, IL-9, and TNF-beta, suggesting an indirect role in averting prolonged virus-induced tissue damage. Collectively, our data show that naïve CD4+ T cell play a critical role in rapid viral T cell control, obviating aberrant antibody and cytokine profiles and disease deterioration. These data may help in guiding risk stratification for severe COVID-19. [ABSTRACT FROM AUTHOR]

Published
2022
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15. Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

Author

López-Labrador, F Xavier, Brown, Julianne R, Fischer, Nicole, Harvala, Heli, Van Boheemen, Sander, Cinek, Ondrej, Sayiner, Arzu, Madsen, Tina Vasehus, Auvinen, Eeva, Kufner, Verena, Huber, Michael, Rodriguez, Christophe, Jonges, Marcel, Hönemann, Mario, Susi, Petri, Sousa, Hugo, Klapper, Paul E, Pérez-Cataluňa, Alba, Hernandez, Marta, Molenkamp, Richard, van der Hoek, Lia, Schuurman, Rob, Couto, Natacha, Leuzinger, Karoline, Simmonds, Peter, Beer, Martin, Höper, Dirk, Kamminga, Sergio, Feltkamp, Mariet C W, Rodríguez-Díaz, Jesús, et al, and University of Zurich

Subjects
10028 Institute of Medical Virology, 2406 Virology, 570 Life sciences, biology, 610 Medicine & health, 2725 Infectious Diseases
Published
2021

17. Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

Author

López-Labrador, F Xavier, Brown, Julianne R, Fischer, Nicole, Harvala, Heli, Van Boheemen, Sander, Cinek, Ondrej, Sayiner, Arzu, Madsen, Tina Vasehus, Auvinen, Eeva, Kufner, Verena, Huber, Michael, Rodriguez, Christophe, Jonges, Marcel, Hönemann, Mario, Susi, Petri, Sousa, Hugo, Klapper, Paul E, Pérez-Cataluña, Alba, Hernandez, Marta, Molenkamp, Richard, der Hoek, Lia van, Schuurman, Rob, Couto, Natacha, Leuzinger, Karoline, Simmonds, Peter, Beer, Martin, Höper, Dirk, Kamminga, Sergio, Feltkamp, Mariet C W, Rodríguez-Díaz, Jesús, Keyaerts, Els, Nielsen, Xiaohui Chen, Puchhammer-Stöckl, Elisabeth, Kroes, Aloys C M, Buesa, Javier, Breuer, Judy, Claas, Eric C J, de Vries, Jutte J C, López-Labrador, F Xavier, Brown, Julianne R, Fischer, Nicole, Harvala, Heli, Van Boheemen, Sander, Cinek, Ondrej, Sayiner, Arzu, Madsen, Tina Vasehus, Auvinen, Eeva, Kufner, Verena, Huber, Michael, Rodriguez, Christophe, Jonges, Marcel, Hönemann, Mario, Susi, Petri, Sousa, Hugo, Klapper, Paul E, Pérez-Cataluña, Alba, Hernandez, Marta, Molenkamp, Richard, der Hoek, Lia van, Schuurman, Rob, Couto, Natacha, Leuzinger, Karoline, Simmonds, Peter, Beer, Martin, Höper, Dirk, Kamminga, Sergio, Feltkamp, Mariet C W, Rodríguez-Díaz, Jesús, Keyaerts, Els, Nielsen, Xiaohui Chen, Puchhammer-Stöckl, Elisabeth, Kroes, Aloys C M, Buesa, Javier, Breuer, Judy, Claas, Eric C J, and de Vries, Jutte J C

Abstract

Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

Published
2020

20. Convalescent Plasma in a Patient with Protracted COVID-19 and Secondary Hypogammaglobulinemia Due to Chronic Lymphocytic Leukemia: Buying Time to Develop Immunity?

Author

Hanssen, Jaap L. J., Stienstra, Johan, Boers, Stefan A., Pothast, Cilia R., Zaaijer, Hans L., Tjon, Jennifer M., Heemskerk, Mirjam H. M., Feltkamp, Mariet C. W., and Arend, Sandra M.

Subjects
CONVALESCENT plasma, CHRONIC lymphocytic leukemia, COMMON variable immunodeficiency, COVID-19, CELLULAR immunity
Abstract

It is not exactly clear yet which type of immune response prevails to accomplish viral clearance in coronavirus disease 2019 (COVID-19). Studying a patient with chronic lymphocytic leukemia and hypogammaglobulinemia who suffered from COVID-19 provided insight in the immunological responses after treatment with COVID-19 convalescent plasma (CCP). Treatment consisted of oxygen, repeated glucocorticosteroids and multiple dosages of CCP guided by antibody levels. Retrospectively performed humoral and cellular immunity analysis made clear that not every serological test for COVID-19 is appropriate for follow-up of sufficient neutralizing antibodies after CCP. In retrospect, we think that CCP merely bought time for this patient to develop an adequate cellular immune response which led to viral clearance and ultimately clinical recovery. [ABSTRACT FROM AUTHOR]

Published
2021
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25. Human papillomavirus and posttransplantation cutaneous squamous cell carcinoma: A multicenter, prospective cohort study

Author

Bavinck, Jan N. Bouwes, Feltkamp, Mariet C. W., Green, Adele C., Fiocco, Marta, Euvrard, Sylvie, Harwood, Catherine A., Nasir, Shaaira, Thomson, Jason, Proby, Charlotte M., Naldi, Luigi, Diphoorn, Janouk C. D., Venturuzzo, Anna, Tessari, Gianpaolo, Nindl, Ingo, Sampogna, Francesca, Abeni, Damiano, Neale, Rachel E., Goeman, Jelle J., Quint, Koen D., Halk, Anne B., Sneek, Carmen, Genders, Roel E., de Koning, Maurits N. C., Quint, Wim G. V., Wieland, Ulrike, Weissenborn, Soenke, Waterboer, Tim, Pawlita, Michael, Pfister, Herbert, Bavinck, Jan N. Bouwes, Feltkamp, Mariet C. W., Green, Adele C., Fiocco, Marta, Euvrard, Sylvie, Harwood, Catherine A., Nasir, Shaaira, Thomson, Jason, Proby, Charlotte M., Naldi, Luigi, Diphoorn, Janouk C. D., Venturuzzo, Anna, Tessari, Gianpaolo, Nindl, Ingo, Sampogna, Francesca, Abeni, Damiano, Neale, Rachel E., Goeman, Jelle J., Quint, Koen D., Halk, Anne B., Sneek, Carmen, Genders, Roel E., de Koning, Maurits N. C., Quint, Wim G. V., Wieland, Ulrike, Weissenborn, Soenke, Waterboer, Tim, Pawlita, Michael, and Pfister, Herbert

Abstract

Organ transplant recipients (OTRs) have a 100-fold increased risk of cutaneous squamous cell carcinoma (cSCC). We prospectively evaluated the association between genus human papillomaviruses (PV) and keratinocyte carcinoma in OTRs. Two OTR cohorts without cSCC were assembled: cohort 1 was transplanted in 2003-2006 (n =274) and cohort 2 was transplanted in 1986-2002 (n =352). Participants were followed until death or cessation of follow-up in 2016. PV infection was assessed in eyebrow hair by using polymerase chain reaction-based methods. PV IgG seroresponses were determined with multiplex serology. A competing risk model with delayed entry was used to estimate cumulative incidence of histologically proven cSCC and the effect of PV by using a multivariable Cox regression model. Results are reported as adjusted hazard ratios (HRs). OTRs with 5 or more different PV types in eyebrow hair had 1.7 times the risk of cSCC vs OTRs with 0 to 4 different types (HR 1.7, 95% confidence interval 1.1-2.6). A similar risk was seen with high PV loads (HR 1.8, 95% confidence interval 1.2-2.8). No significant associations were seen between serum antibodies and cSCC or between PV and basal cell carcinoma. The diversity and load of PV types in eyebrow hair are associated with cSCC risk in OTRs, providing evidence that PV is associated with cSCC carcinogenesis and may present a target for future preventive strategies. In two cohorts of organ transplant recipients, those with five and more different beta-genus human papillomavirus types and high virus loads in eyebrow hairs subsequently develop significantly more cutaneous squamous cell carcinomas than others, suggesting that these virus types may increase the risk of cutaneous squamous cell carcinoma in these patients.

Published
2018

29. Clinically Relevant Reactivation of Polyomavirus BK (BKPyV) in HLA-A02-Positive Renal Transplant Recipients Is Associated with Impaired Effector-Memory Differentiation of BKPyV-Specific CD8+ T Cells

Author

van Aalderen, Michiel C., primary, Remmerswaal, Ester B. M., additional, Heutinck, Kirstin M., additional, ten Brinke, Anja, additional, Feltkamp, Mariet C. W., additional, van der Weerd, Neelke C., additional, van der Pant, Karlijn A. M. I., additional, Bemelman, Frederike J., additional, van Lier, René A. W., additional, and ten Berge, Ineke J. M., additional

Published
2016
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30. Primary Polyomavirus Infection, Not Reactivation, as the Cause of Trichodysplasia Spinulosa in Immunocompromised Patients

Author

van der Meijden, Els, primary, Horváth, Barbara, additional, Nijland, Marcel, additional, de Vries, Karin, additional, Rácz, Emoőke, additional, Diercks, Gilles F., additional, de Weerd, Annelies E., additional, Clahsen-van Groningen, Marian C., additional, van der Blij-de Brouwer, Caroline S., additional, van der Zon, Arnulfo J., additional, Kroes, Aloys C. M., additional, Hedman, Klaus, additional, van Kampen, Jeroen J. A., additional, Riezebos-Brilman, Annelies, additional, and Feltkamp, Mariet C. W., additional

Published
2016
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34. Human papillomavirus and posttransplantation cutaneous squamous cell carcinoma: A multicenter, prospective cohort study

Author

Bouwes Bavinck, Jan N., Feltkamp, Mariet C. W., Green, Adele C., Fiocco, Marta, Euvrard, Sylvie, Harwood, Catherine A., Nasir, Shaaira, Thomson, Jason, Proby, Charlotte M., Naldi, Luigi, Diphoorn, Janouk C. D., Venturuzzo, Anna, Tessari, Gianpaolo, Nindl, Ingo, Sampogna, Francesca, Abeni, Damiano, Neale, Rachel E., Goeman, Jelle J., Quint, Koen D., Halk, Anne B., Sneek, Carmen, Genders, Roel E., de Koning, Maurits N. C., Quint, Wim G. V., Wieland, Ulrike, Weissenborn, Sönke, Waterboer, Tim, Pawlita, Michael, Pfister, Herbert, Zwan‐Kralt, P., Graaf, Y. G. L., Vos, L. E., Uphoff‐Meijerink, E. J., Willemze, R., Struijk, L., Wanningen, P., Meijden, E., Plasmeijer, E. I., Wolterbeek, R., Ocampo, A. C. M. A., Kanitakis, J., Stockfleth, E., Forschner, T., Pizzagalli, A., Sassi, F., Gotti, E., Fiocchi, R., Breuer, J., Mitchell, L., Purdie, K., Lambert, S. R., Ran, H., Sehr, P., Michael, K. M., Schegget, J., Kleter, B., Doorn, L. J., Simoni, S., Petasecca Donati, G. P., Masini, C., Olsen, C., O’Rourke, P., Harrison, S., and Buttner, P.

Abstract

Organ transplant recipients (OTRs) have a 100‐fold increased risk of cutaneous squamous cell carcinoma (cSCC). We prospectively evaluated the association between β genus human papillomaviruses (βPV) and keratinocyte carcinoma in OTRs. Two OTRcohorts without cSCCwere assembled: cohort 1 was transplanted in 2003‐2006 (n = 274) and cohort 2 was transplanted in 1986‐2002 (n = 352). Participants were followed until death or cessation of follow‐up in 2016. βPVinfection was assessed in eyebrow hair by using polymerase chain reaction–based methods. βPVIgG seroresponses were determined with multiplex serology. A competing risk model with delayed entry was used to estimate cumulative incidence of histologically proven cSCCand the effect of βPVby using a multivariable Cox regression model. Results are reported as adjusted hazard ratios (HRs). OTRswith 5 or more different βPVtypes in eyebrow hair had 1.7 times the risk of cSCCvs OTRs with 0 to 4 different types (HR1.7, 95% confidence interval 1.1‐2.6). A similar risk was seen with high βPVloads (HR1.8, 95% confidence interval 1.2‐2.8). No significant associations were seen between serum antibodies and cSCCor between βPVand basal cell carcinoma. The diversity and load of βPVtypes in eyebrow hair are associated with cSCCrisk in OTRs, providing evidence that βPVis associated with cSCCcarcinogenesis and may present a target for future preventive strategies. In two cohorts of organ transplant recipients, those with five and more different beta‐genus human papillomavirus types and high virus loads in eyebrow hairs subsequently develop significantly more cutaneous squamous cell carcinomas than others, suggesting that these virus types may increase the risk of cutaneous squamous cell carcinoma in these patients.

Published
2018
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38. Clinically Relevant Reactivation of Polyomavirus BK (BKPyV) in HLA-A02-Positive Renal Transplant Recipients Is Associated with Impaired Effector-Memory Differentiation of BKPyV-Specific CD8+ T Cells.

Author

van Aalderen, Michiel C., Remmerswaal, Ester B. M., Heutinck, Kirstin M., ten Brinke, Anja, Feltkamp, Mariet C. W., van der Weerd, Neelke C., van der Pant, Karlijn A. M. I., Bemelman, Frederike J., van Lier, René A. W., and ten Berge, Ineke J. M.

Subjects
POLYOMAVIRUS diseases, KIDNEY transplant patients, POLYOMAVIRUSES, T cell differentiation, CD81 antigen, DISEASE risk factors
Abstract

Polyomavirus BK (BKPyV) frequently reactivates in immunosuppressed renal transplant recipients (RTRs) and may lead to graft loss due to BKPyV-induced interstitial nephritis (BKVN). Little is known on the differentiation of CD8+ T cells targeting BKPyV in RTRs. Here we investigated whether BKPyV-specific CD8+ T cell differentiation differs in RTRs with varying degrees of BKPyV reactivation and/or BKVN. Using combinatorial encoding with tetramers carrying BKPyV major capsid protein (VP1) and large T antigen protein (LTAG) epitopes, we investigated CD8+ T cell responses to BKPyV in longitudinally obtained PBMC samples from 46 HLA-A02-positive RTRs and 20 healthy adults. We were also able to isolate BKPyV-specific CD8+ T cells from five renal allografts, two of which were affected by BKVN. Before transplantation, BKPyV-specific CD8+ T cells targeting VP1 and LTAG epitopes appeared predominantly as central-memory and CD27+/CD28+ effector-memory (TEM), and naïve-like PD-1-expressing cells, respectively. After viral reactivation, BKPyV-specific CD8+ T cells assumed CD28− TEM and TEMRA states in patients who were able to control BKPyV, whereas differentiation lagged behind in patients with severe viral reactivation or BKVN. Furthermore, VP1-specific CD69+/CD103+ tissue-resident memory (TRM) cells accumulated in BKVN-affected allografts but lacked signs of effector differentiation. In contrast, granzyme B-expressing effector cells were detected in allografts not affected by BKVN. In conclusion, effector-memory differentiation of BKPyV-specific CD8+ T cells in patients with high viral load or BKVN is impaired. Further characterization of the specific mechanisms behind this altered cellular differentiation is necessary to develop therapies that can prevent the emergence of BKVN. [ABSTRACT FROM AUTHOR]

Published
2016
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40. Lack of association between the presence and persistence of betapapillomavirus DNA in eyebrow hairs and betapapillomavirus L1 antibodies in serum

Author

Plasmeijer, Elsemieke I., primary, Neale, Rachel E., additional, O'Rourke, Peter, additional, Mallitt, Kylie-Ann, additional, de Koning, Maurits N. C., additional, Quint, Wim, additional, Buettner, Petra G., additional, Pawlita, Michael, additional, Waterboer, Tim, additional, Green, Adele C., additional, and Feltkamp, Mariet C. W., additional

Published
2010
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42. Evaluation of a Novel Broad-Spectrum PCR-Multiplex Genotyping Assay for Identification of Cutaneous Wart-Associated Human Papillomavirus Types

Author

de Koning, Maurits N. C., primary, ter Schegget, Jan, additional, Eekhof, Just A. H., additional, Kamp, Marga, additional, Kleter, Bernhard, additional, Gussekloo, Jacobijn, additional, Feltkamp, Mariet C. W., additional, Bouwes Bavinck, Jan Nico, additional, Purdie, Karin J., additional, Bunker, Christopher B., additional, Proby, Charlotte M., additional, Meys, Rhonda, additional, Harwood, Catherine A., additional, and Quint, Wim G. V., additional

Published
2010
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47. Human Polyomavirus 9 Infection in Kidney Transplant Patients.

Author

van der Meijden, Els, Wunderink, Herman F., van der Blij-de Brouwer, Caroline S., Zaaijer, Hans L., Rotmans, Joris I., Bavinck, Jan Nico Bouwes, and Feltkamp, Mariet C. W.

Subjects
POLYOMAVIRUS diseases, KIDNEY transplantation, POLYOMAVIRUSES, IMMUNOSUPPRESSION, IMMUNOSUPPRESSIVE agents, VIRAL genetics
Abstract

Several human polyomaviruses of unknown prevalence and pathogenicity have been identified, including human polyomavirus 9 (HPyV9). To determine rates of HPyV9 infection among immunosuppressed patients, we screened serum samples from 101 kidney transplant patients in the Netherlands for HPyV9 DNA and seroreactivity. A total of 21 patients had positive results for HPyV9 DNA; positivity rates peaked at 3 months after transplantation, but the highest viral loads were measured just after transplantation. During 18 months of follow-up, HPyV9 seroprevalence increased from 33% to 46% among transplant patients; seroprevalence remained stable at ≈30% in a control group of healthy blood donors in whom no HPyV9 DNA was detected. Further analysis revealed an association between detection of HPyV9 and detection of BK polyomavirus but not of cytomegalovirus. Our data indicate that HPyV9 infection is frequent in kidney transplant patients, but the nature of infection--endogenous or donor-derived--and pathogenic potential of this virus remain unknown. [ABSTRACT FROM AUTHOR]

Published
2014
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50. Human Papillomavirus Load in Eyebrow Hair Follicles and Risk of Cutaneous Squamous Cell Carcinoma.

Author

Neale, Rachel E., Weissenborn, Soenke, Abeni, Damiano, Bouwes Bavinck, Jan Nico, Euvrard, Sylvie, Feltkamp, Mariet C. W., Green, Adele C., Harwood, Catherine, de Koning, Maurits, Naldi, Luigi, Nindi, Ingo, Pawlita, Michael, Proby, Charlotte, Quint, Wim G., Waterboer, Tim, Wieland, Ulrike, and Pfister, Herbert

Abstract

The article discusses the role of viral load in the development of skin cancer using the findings of a case-control study conducted to determine the association between the load of 8 beta-human papillomavirus (betaPV) types and risk of squamous cell carcinomas (SCCs). Topics covered include the process of selecting the study participants, the statistical methods used to express the viral load and determined differences between the case participants and control participants.

Published
2013
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