1. Role of PRPS2 as a prognostic and therapeutic target in osteosarcoma.
- Author
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Luo Y, Yuan J, Huang J, Yang T, Zhou J, Tang J, Liu M, Chen J, Chen C, Huang W, and Zhang H
- Subjects
- Bone Neoplasms mortality, Bone Neoplasms pathology, Bone Neoplasms surgery, Cell Proliferation, Child, Child, Preschool, Female, Femoral Neoplasms mortality, Femoral Neoplasms pathology, Femoral Neoplasms surgery, Humans, Immunohistochemistry, Infant, Ki-67 Antigen analysis, Male, Neoplasm Recurrence, Local, Osteosarcoma mortality, Osteosarcoma pathology, Osteosarcoma surgery, Phosphorylation, TOR Serine-Threonine Kinases analysis, Tissue Array Analysis, Treatment Outcome, Bone Neoplasms enzymology, Femoral Neoplasms enzymology, Osteosarcoma enzymology, Ribose-Phosphate Pyrophosphokinase analysis
- Abstract
Aims: Osteosarcoma (OS) is the most common primary malignant tumour of the bone. However, further improvement in survival has not been achieved due to a lack of well-validated prognostic markers and more effective therapeutic agents. Recently, the c-Myc-phosphoribosyl pyrophosphate synthetase 2 (PRPS2) pathway has been shown to promote nucleic acid metabolism and cancer cell proliferation in malignant melanoma; phosphorylated mammalian target of rapamycin (p-mTOR) has been upregulated and an effective therapeutic target in OS. However, the p-mTOR-PRPS2 pathway has not been evaluated in OS., Methods: In this study, the expression level of PRPS2, p-mTOR and marker of proliferation (MKI-67) was observed in a cohort of specimens (including 236 OS cases and 56 control samples) using immunohistochemistry, and the association between expression level and clinicopathological characteristics of patients with OS was analysed., Results: PRPS2 protein level, which is related to tumour proliferation, was higher in OS cells (p=0.003) than in fibrous dysplasia, and the higher PRPS2 protein level was associated with a higher tumour recurrence (p=0.001). In addition, our statistical analysis confirmed that PRPS2 is a novel, independent prognostic indicator of OS. Finally, we found that the expression of p-mTOR was associated with the poor prognosis of patients with OS (p<0.05)., Conclusions: PRPS2 is an independent prognostic marker and a potential therapeutic target for OS., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2021
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