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Deoxynivalenol (DON) and fumonisin B 1 (FB 1 ), as a group of highly toxic secondary metabolites, have become a potential source of water environmental pollutants. To minimize two mycotoxins exposure to consumers, a dual lateral flow immunoassay (LFIA) integrated with the smartphone was reported for simultaneous and quantitative detection of DON and FB 1 in the water environment. The significantly improved sensitivity was contributed to a smartphone-based device with the ability to image and analyze results. Under optimized conditions, the detection limits of DON and FB 1 were calculated to be 3.46 and 2.65 ng/mL, which were approximately 25 and 10 folds lower than those of the visual detection of the LFIA. This method showed good specificity and a good dynamic linear detection for DON and FB 1 . The recoveries of DON and FB 1 were evaluated by the spiked lake water, river water, and pond water, ranging from 92.47% to 106.2% with the relative standard deviation under 9.13%. Moreover, the results of the developed LFIA showed a high correlation with enzyme-linked immunosorbent assay (ELISA) results, with a correlation coefficient of 0.999 for DON and 0.996 for FB 1 , respectively. To sum up, the developed LFIA provides a promising platform for sensitive, simultaneous, quantitative, and on-site detection of DON and FB 1 in the water environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022. Published by Elsevier B.V.)