141 results on '"Feng JC"'
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2. Numerical Simulation of the Influence of Interfacial Characteristics of Bulletproof Plates on Ballistic Protection Performance
- Author
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Gao, B, primary, Peng, G, additional, Wang, XC, additional, Zhao, H, additional, Feng, JC, additional, Chen, CX, additional, and Zhang, B, additional
- Published
- 2023
- Full Text
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3. Analysis of the Ballistic Performance and Mechanism of Soft Bulletproof Layers under the Impact of Multiple Lead Core Bullets
- Author
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Wang, XC, primary, Wang, W, additional, Peng, G, additional, Chen, CX, additional, Gao, B, additional, Feng, JC, additional, and Zhang, B, additional
- Published
- 2023
- Full Text
- View/download PDF
4. Analysis Model for Damage of PBO Composite Laminate Target by High-Velocity Projectile Penetration
- Author
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Chen, CX, primary, Wang, XC, additional, Peng, G, additional, Wang, W, additional, Gao, B, additional, Feng, JC, additional, and Zhang, B, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Analysis of influencing factors of projectile velocity in low-speed sub-caliber launch
- Author
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Zhang, B, primary, Peng, G, additional, Feng, JC, additional, Wang, XC, additional, Wang, W, additional, Chen, CX, additional, and Gao, B, additional
- Published
- 2023
- Full Text
- View/download PDF
6. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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Klionsky, DJ, Abdel-Aziz, AK, Abdelfatah, S, Abdellatif, M, Abdoli, A, Abel, S, Abeliovich, H, Abildgaard, MH, Abudu, YP, Acevedo-Arozena, A, Adamopoulos, IE, Adeli, K, Adolph, TE, Adornetto, A, Aflaki, E, Agam, G, Agarwal, A, Aggarwal, BB, Agnello, M, Agostinis, P, Agrewala, JN, Agrotis, A, Aguilar, PV, Ahmad, ST, Ahmed, ZM, Ahumada-Castro, U, Aits, S, Aizawa, S, Akkoc, Y, Akoumianaki, T, Akpinar, HA, Al-Abd, AM, Al-Akra, L, Al-Gharaibeh, A, Alaoui-Jamali, MA, Alberti, S, Alcocer-Gomez, E, Alessandri, C, Ali, M, Al-Bari, MAA, Aliwaini, S, Alizadeh, J, Almacellas, E, Almasan, A, Alonso, A, Alonso, GD, Altan-Bonnet, N, Altieri, DC, Alves, S, da Costa, CA, Alzaharna, MM, Amadio, M, Amantini, C, Amaral, C, Ambrosio, S, Amer, AO, Ammanathan, V, An, ZY, Andersen, SU, Andrabi, SA, Andrade-Silva, M, Andres, AM, Angelini, S, Ann, D, Anozie, UC, Ansari, MY, Antas, P, Antebi, A, Anton, Z, Anwar, T, Apetoh, L, Apostolova, N, Araki, T, Araki, Y, Arasaki, K, Araujo, WL, Araya, J, Arden, C, Arevalo, MA, Arguelles, S, Arias, E, Arikkath, J, Arimoto, H, Ariosa, AR, Armstrong-James, D, Arnaune-Pelloquin, L, Aroca, A, Arroyo, DS, Arsov, I, Artero, R, Asaro, DML, Aschner, M, Ashrafizadeh, M, Ashur-Fabian, O, Atanasov, AG, Au, AK, Auberger, P, Auner, HW, Aurelian, L, Autelli, R, Avagliano, L, Avalos, Y, Aveic, S, Aveleira, CA, AvinWittenberg, T, Aydin, Y, Ayton, S, Ayyadevara, S, Azzopardi, M, Baba, M, Backer, JM, Backues, SK, Bae, DH, Bae, ON, Bae, SH, Baehrecke, EH, Baek, A, Baek, SH, Bagetta, G, Bagniewska-Zadworna, A, Bai, H, Bai, J, Bai, XY, Bai, YD, Bairagi, N, Baksi, S, Balbi, T, Baldari, CT, Balduini, W, Ballabio, A, Ballester, M, Balazadeh, S, Balzan, R, Bandopadhyay, R, Banerjee, S, Bao, Y, Baptista, MS, Baracca, A, Barbati, C, Bargiela, A, Barila, D, Barlow, PG, Barmada, SJ, Barreiro, E, Barreto, GE, Bartek, J, Bartel, B, Bartolome, A, Barve, GR, Basagoudanavar, SH, Bassham, DC, Jr, RCB, Basu, A, Batoko, H, Batten, I, Baulieu, EE, Baumgarner, BL, Bayry, J, Beale, R, Beau, I, Beaumatin, F, Bechara, LRG, Beck, GR, Beers, MF, Begun, J, Behrends, C, Behrens, GMN, Bei, R, Bejarano, E, Bel, S, Behl, C, Belaid, A, Belgareh-Touze, N, Bellarosa, C, Belleudi, F, Perez, MB, Bello-Morales, R, Beltran, JSD, Beltran, S, Benbrook, DM, Bendorius, M, Benitez, BA, Benito-Cuesta, I, Bensalem, J, Berchtold, MW, Berezowska, S, Bergamaschi, D, Bergami, M, Bergmann, A, Berliocchi, L, Berlioz-Torrent, C, Bernard, A, Berthoux, L, Besirli, CG, Besteiro, S, Betin, VM, Beyaert, R, Bezbradica, JS, Bhaskar, K, Bhatia-Kissova, I, Bhattacharya, R, Bhattacharya, S, Bhattacharyya, S, Bhuiyan, MS, Bhutia, SK, Bi, LR, Bi, XL, Biden, TJ, Bijian, K, Billes, VA, Binart, N, Bincoletto, C, Birgisdottir, AB, Bjorkoy, G, Blanco, G, Blas-Garcia, A, Blasiak, J, Blomgran, R, Blomgren, K, Blum, JS, Boada-Romero, E, Boban, M, BoeszeBattaglia, K, Boeuf, P, Boland, B, Bomont, P, Bonaldo, P, Bonam, SR, Bonfili, L, Bonifacino, JS, Boone, BA, Bootman, MD, Bordi, M, Borner, C, Bornhauser, BC, Borthakur, G, Bosch, J, Bose, S, Botana, LM, Botas, J, Boulanger, CM, Boulton, ME, Bourdenx, M, Bourgeois, B, Bourke, NM, Bousquet, G, Boya, P, Bozhkov, PV, Bozi, LHM, Bozkurt, TO, Brackney, DE, Brandts, CH, Braun, RJ, Braus, GH, Bravo-Sagua, R, Bravo-San Pedro, JM, Brest, P, Bringer, MA, Briones-Herrera, A, Broaddus, VC, Brodersen, P, Alvarez, EMC, Brodsky, JL, Brody, SL, Bronson, PG, Bronstein, JM, Brown, CN, Brown, RE, Brum, PC, Brumell, JH, Brunetti-Pierri, N, Bruno, D, Bryson-Richardson, RJ, Bucci, C, Buchrieser, C, Bueno, M, Buitrago-Molina, LE, Buraschi, S, Buch, S, Buchan, JR, Buckingham, EM, Budak, H, Budini, M, Bultynck, G, Burada, F, Burgoyne, JR, Buron, MI, Bustos, V, Buttner, S, Butturini, E, Byrd, A, Cabas, I, Cabrera-Benitez, S, Cadwell, K, Cai, JJ, Cai, L, Cai, Q, Cairo, M, Calbet, JA, Caldwell, GA, Caldwell, KA, Call, JA, Calvani, R, Calvo, AC, Barrera, MCR, Camara, NO, Camonis, JH, Camougrand, N, Campanella, M, Campbell, EM, Campbell-Valois, FX, Campello, S, Campesi, I, Campos, JC, Camuzard, O, Cancino, J, de Almeida, DC, Canesi, L, Caniggia, I, Canonico, B, Canti, C, Cao, B, Caraglia, M, Carames, B, Carchman, EH, Cardenal-Munoz, E, Cardenas, C, Cardenas, L, Cardoso, SM, Carew, JS, Carle, GF, Carleton, G, Carloni, S, Carmona-Gutierrez, D, Carneiro, LA, Carnevali, O, Carosi, JM, Carra, S, Carrier, A, Carrier, L, Carroll, B, Carter, AB, Carvalho, AN, Casanova, M, Casas, C, Casas, J, Cassioli, C, Castillo, EF, Castillo, K, Castillo-Lluva, S, Castoldi, F, Castori, M, Castro, AF, Castro-Caldas, M, Castro-Hernandez, J, Castro-Obregon, S, Catz, SD, Cavadas, C, Cavaliere, F, Cavallini, G, Cavinato, M, Cayuela, ML, Rica, PC, Cecarini, V, Cecconi, F, Cechowska-Pasko, M, Cenci, S, Ceperuelo-Mallafre, V, Cerqueira, JJ, Cerutti, JM, Cervia, D, Cetintas, VB, Cetrullo, S, Chae, HJ, Chagin, AS, Chai, CY, Chakrabarti, G, Chakrabarti, O, Chakraborty, T, Chami, M, Chamilos, G, Chan, DW, Chan, EYW, Chan, ED, Chan, HYE, Chan, HH, Chan, H, Chan, MTV, Chan, YS, Chandra, PK, Chang, CP, Chang, CM, Chang, HC, Chang, K, Chao, J, Chapman, T, Charlet-Berguerand, N, Chatterjee, S, Chaube, SK, Chaudhary, A, Chauhan, S, Chaum, E, Checler, F, Cheetham, ME, Chen, CS, Chen, GC, Chen, JF, Chen, LL, Chen, L, Chen, ML, Chen, MK, Chen, N, Chen, Q, Chen, RH, Chen, S, Chen, W, Chen, WQ, Chen, XM, Chen, XW, Chen, X, Chen, Y, Chen, YG, Chen, YY, Chen, YQ, Chen, YJ, Chen, ZS, Chen, Z, Chen, ZH, Chen, ZJ, Chen, ZX, Cheng, HH, Cheng, J, Cheng, SY, Cheng, W, Cheng, XD, Cheng, XT, Cheng, YY, Cheng, ZY, Cheong, H, Cheong, JK, Chernyak, BV, Cherry, S, Cheung, CFR, Cheung, CHA, Cheung, KH, Chevet, E, Chi, RJ, Chiang, AKS, Chiaradonna, F, Chiarelli, R, Chiariello, M, Chica, N, Chiocca, S, Chiong, M, Chiou, SH, Chiramel, AI, Chiurchiu, V, Cho, DH, Choe, SK, Choi, AMK, Choi, ME, Choudhury, KR, Chow, NS, Chu, CT, Chua, JP, Chua, JJE, Chung, H, Chung, KP, Chung, S, Chung, SH, Chung, YL, Cianfanelli, V, Ciechomska, IA, Cifuentes, M, Cinque, L, Cirak, S, Cirone, M, Clague, MJ, Clarke, R, Clementi, E, Coccia, EM, Codogno, P, Cohen, E, Cohen, MM, Colasanti, T, Colasuonno, F, Colbert, RA, Colell, A, Coll, NS, Collins, MO, Colombo, MI, Colon-Ramos, DA, Combaret, L, Comincini, S, Cominetti, MR, Consiglio, A, Conte, A, Conti, F, Contu, VR, Cookson, MR, Coombs, KM, Coppens, I, Corasaniti, MT, Corkery, DP, Cordes, N, Cortese, K, Costa, MD, Costantino, S, Costelli, P, Coto-Montes, A, Crack, PJ, Crespo, JL, Criollo, A, Crippa, V, Cristofani, R, Csizmadia, T, Cuadrado, A, Cui, B, Cui, J, Cui, YX, Cui, Y, Culetto, E, Cumino, AC, Cybulsky, AV, Czaja, MJ, Czuczwar, SJ, D'Adamo, S, D'Amelio, M, D'Arcangelo, D, D'Lugos, AC, D'Orazi, G, da Silva, JA, Dafsari, HS, Dagda, RK, Dagdas, Y, Daglia, M, Dai, X, Dai, Y, Dai, YY, Dal Col, J, Dalhaimer, P, Dalla Valle, L, Dallenga, T, Dalmasso, G, Damme, M, Dando, I, Dantuma, NP, Darling, AL, Das, H, Dasarathy, S, Dasari, SK, Dash, S, Daumke, O, Dauphinee, AN, Davies, JS, Davila, VA, Davis, RJ, Davis, T, Naidu, SD, De Amicis, F, De Bosscher, K, De Felice, F, De Franceschi, L, De Leonibus, C, Barbosa, MGD, De Meyer, GRY, De Milito, A, De Nunzio, C, De Palma, C, De Santi, M, De Virgilio, C, De Zio, D, Debnath, J, DeBosch, BJ, Decuypere, J, Deehan, MA, Deflorian, G, DeGregori, J, Dehay, B, Del Rio, G, Delaney, JR, Delbridge, LMD, Delorme-Axford, E, Delpino, MV, Demarchi, F, Dembitz, V, Demers, ND, Deng, HB, Deng, ZQ, Dengjel, J, Dent, P, Denton, D, DePamphilis, ML, Der, CJ, Deretic, V, Descoteaux, A, Devis, L, Devkota, S, Devuyst, O, Dewson, G, Dharmasivam, M, Dhiman, R, di Bernardo, D, Di Cristina, M, Di Domenico, F, Di Fazio, P, Di Fonzo, A, Di Guardo, G, Di Guglielmo, GM, Di Leo, L, Di Malta, C, Di Nardo, A, Di Rienzo, M, Di Sano, F, Diallinas, G, Diao, JJ, Diaz-Araya, G, Diaz-Laviada, I, Dickinson, JM, Diederich, M, Dieude, M, Dikic, I, Ding, SP, Ding, WX, Dini, L, Dinic, M, Dinkova-Kostova, AT, Dionne, MS, Distler, JHW, Diwan, A, Dixon, IMC, Djavaheri-Mergny, M, Dobrinski, I, Dobrovinskaya, O, Dobrowolski, R, Dobson, RCJ, Emre, SD, Donadelli, M, Dong, B, Dong, XN, Dong, ZW, Ii, GWD, Dotsch, V, Dou, H, Dou, J, Dowaidar, M, Dridi, S, Drucker, L, Du, AL, Du, CG, Du, GW, Du, HN, Du, LL, du Toit, A, Duan, SB, Duan, XQ, Duarte, SP, Dubrovska, A, Dunlop, EA, Dupont, N, Duran, RV, Dwarakanath, BS, Dyshlovoy, SA, Ebrahimi-Fakhari, D, Eckhart, L, Edelstein, CL, Efferth, T, Eftekharpour, E, Eichinger, L, Eid, N, Eisenberg, T, Eissa, NT, Eissa, S, Ejarque, M, El Andaloussi, A, El-Hage, N, El-Naggar, S, Eleuteri, AM, El-Shafey, ES, Elgendy, M, Eliopoulos, AG, Elizalde, MM, Elks, PM, Elsasser, HP, Elsherbiny, ES, Emerling, BM, Emre, NCT, Eng, CH, Engedal, N, Engelbrecht, AM, Engelsen, AST, Enserink, JM, Escalante, R, Esclatine, A, Escobar-Henriques, M, Eskelinen, EL, Espert, L, Eusebio, MO, Fabrias, G, Fabrizi, C, Facchiano, A, Facchiano, F, Fadeel, B, Fader, C, Faesen, AC, Fairlie, WD, Falco, A, Falkenburger, BH, Fan, DP, Fan, J, Fan, YB, Fang, EF, Fang, YS, Fang, YQ, Fanto, M, Farfel-Becker, T, Faure, M, Fazeli, G, Fedele, AO, Feldman, AM, Feng, D, Feng, JC, Feng, LF, Feng, YB, Feng, YC, Feng, W, Araujo, TF, Ferguson, TA, Fernandez-Checa, JC, FernandezVeledo, S, Fernie, AR, Ferrante, AW, Ferraresi, A, Ferrari, MF, Ferreira, JCB, Ferro-Novick, S, Figueras, A, Filadi, R, Filigheddu, N, FilippiChiela, E, Filomeni, G, Fimia, GM, Fineschi, V, Finetti, F, Finkbeiner, S, Fisher, EA, Fisher, PB, Flamigni, F, Fliesler, SJ, Flo, TH, Florance, I, Florey, O, Florio, T, Fodor, E, Follo, C, Fon, EA, Forlino, A, Fornai, F, Fortini, P, Fracassi, A, Fraldi, A, Franco, B, Franco, R, Franconi, F, Frankel, LB, Friedman, SL, Frohlich, LF, Fruhbeck, G, Fuentes, JM, Fujiki, Y, Fujita, N, Fujiwara, Y, Fukuda, M, Fulda, S, Furic, L, Furuya, N, Fusco, C, Gack, MU, Gaffke, L, Galadari, S, Galasso, A, Galindo, MF, Kankanamalage, SG, Galluzzi, L, Galy, V, Gammoh, N, Gan, BY, Ganley, IG, Gao, F, Gao, H, Gao, MH, Gao, P, Gao, SJ, Gao, WT, Gao, XB, Garcera, A, Garcia, MN, Garcia, VE, Garcia-Del Portillo, F, Garcia-Escudero, V, GarciaGarcia, A, Garcia-Macia, M, Garcia-Moreno, D, Garcia-Ruiz, C, Garcia-Sanz, P, Garg, AD, Gargini, R, Garofalo, T, Garry, RF, Gassen, NC, Gatica, D, Ge, L, Ge, WZ, Geiss-Friedlander, R, Gelfi, C, Genschik, P, Gentle, IE, Gerbino, V, Gerhardt, C, Germain, K, Germain, M, Gewirtz, DA, Afshar, EG, Ghavami, S, Ghigo, A, Ghosh, M, Giamas, G, Giampietri, C, Giatromanolaki, A, Gibson, GE, Gibson, SB, Ginet, V, Giniger, E, Giorgi, C, Girao, H, Girardin, SE, Giridharan, M, Giuliano, S, Giulivi, C, Giuriato, S, Giustiniani, J, Gluschko, A, Goder, V, Goginashvili, A, Golab, J, Goldstone, DC, Golebiewska, A, Gomes, LR, Gomez, R, Gomez-Sanchez, R, Gomez-Puerto, MC, Gomez-Sintes, R, Gong, Q, Goni, FM, Gonzalez-Gallego, J, Gonzalez-Hernandez, T, Gonzalez-Polo, RA, Gonzalez-Reyes, JA, Gonzalez-Rodriguez, P, Goping, IS, Gorbatyuk, MS, Gorbunov, NV, Gorojod, RM, Gorski, SM, Goruppi, S, Gotor, C, Gottlieb, RA, Gozes, I, Gozuacik, D, Graef, M, Graler, MH, Granatiero, V, Grasso, D, Gray, JP, Green, DR, Greenhough, A, Gregory, SL, Griffin, EF, Grinstaff, MW, Gros, F, Grose, C, Gross, AS, Gruber, F, Grumati, P, Grune, T, Gu, XY, Guan, JL, Guardia, CM, Guda, K, Guerra, F, Guerri, C, Guha, P, Guillen, C, Gujar, S, Gukovskaya, A, Gukovsky, I, Gunst, J, Gunther, A, Guntur, AR, Guo, CY, Guo, C, Guo, HQ, Guo, LW, Guo, M, Gupta, P, Fernandez, AF, Gupta, SK, Gupta, S, Gupta, VB, Gupta, V, Gustafsson, AB, Gutterman, DD, Ranjitha, HB, Haapasalo, A, Haber, JE, Hadano, S, Hafren, AJ, Haidar, M, Hall, BS, Hallden, G, Hamacher-Brady, A, Hamann, A, Hamasaki, M, Han, WD, Hansen, M, Hanson, PI, Hao, ZJ, Harada, M, Harhaji-Trajkovic, L, Hariharan, N, Haroon, N, Harris, J, Hasegawa, T, Nagoor, NH, Haspel, JA, Haucke, V, Hawkins, WD, Hay, BA, Haynes, CM, Hayrabedyan, SB, Hays, TS, He, CC, He, Q, He, RR, He, YW, He, YY, Heakal, Y, Heberle, AM, Hejtmancik, JF, Helgason, GV, Henkel, V, Herb, M, Hergovich, A, Herman-Antosiewicz, A, Hernandez, A, Hernandez, C, Hernandez-Diaz, S, Hernandez-Gea, V, Herpin, A, Herreros, J, Hervas, JH, Hesselson, D, Hetz, C, Heussler, VT, Higuchi, Y, Hilfiker, S, Hill, JA, Hlavacek, WS, Ho, EA, Ho, IHT, Ho, PWL, Ho, S, Ho, WY, Hobbs, GA, Hochstrasser, M, Hoet, PHM, Hofius, D, Hofman, P, Hohn, A, Holmberg, CI, Hombrebueno, JR, Hong, CW, Hong, YR, Hooper, LV, Hoppe, T, Horos, R, Hoshida, Y, Hsin, IL, Hsu, HY, Hu, B, Hu, D, Hu, LF, Hu, MC, Hu, RG, Hu, W, Hu, YC, Hu, ZW, Hua, F, Hua, JL, Hua, YQ, Huan, CM, Huang, CH, Huang, CS, Huang, CX, Huang, CL, Huang, HS, Huang, K, Huang, MLH, Huang, R, Huang, S, Huang, TZ, Huang, X, Huang, YJ, Huber, TB, Hubert, V, Hubner, CA, Hughes, SM, Hughes, WE, Humbert, M, Hummer, G, Hurley, JH, Hussain, S, Hussey, PJ, Hutabarat, M, Hwang, HY, Hwang, S, Ieni, A, Ikeda, F, Imagawa, Y, Imai, Y, Imbriano, C, Imoto, M, Inman, DM, Inoki, K, Iovanna, J, Iozzo, RV, Ippolito, G, Irazoqui, JE, Iribarren, P, Ishaq, M, Ishikawa, M, Ishimwe, N, Isidoro, C, Ismail, N, Issazadeh-Navikas, S, Itakura, E, Ito, D, Ivankovic, D, Ivanova, S, Iyer, AKV, Izquierdo, JM, Izumi, M, Jaattela, M, Jabir, MS, Jackson, WT, Jacobo-Herrera, N, Jacomin, AC, Jacquin, E, Jadiya, P, Jaeschke, H, Jagannath, C, Jakobi, AJ, Jakobsson, J, Janji, B, JansenDurr, P, Jansson, PJ, Jantsch, J, Januszewski, S, Jassey, A, Jean, S, JeltschDavid, H, Jendelova, P, Jenny, A, Jensen, TE, Jessen, N, Jewell, JL, Ji, J, Jia, LJ, Jia, R, Jiang, LW, Jiang, Q, Jiang, RC, Jiang, T, Jiang, XJ, Jiang, Y, Jimenez-Sanchez, M, Jin, EJ, Jin, FY, Jin, HC, Jin, L, Jin, LQ, Jin, MY, Jin, S, Jo, EK, Joffre, C, Johansen, T, Johnson, GVW, Johnston, SA, Jokitalo, E, Jolly, MK, Joosten, LAB, Jordan, J, Joseph, B, Ju, DW, Ju, JS, Ju, JF, Juarez, E, Judith, D, Juhasz, G, Jun, Y, Jung, CH, Jung, S, Jung, YK, Jungbluth, H, Jungverdorben, J, Just, S, Kaarniranta, K, Kaasik, A, Kabuta, T, Kaganovich, D, Kahana, A, Kain, R, Kajimura, S, Kalamvoki, M, Kalia, M, Kalinowski, DS, Kaludercic, N, Kalvari, I, Kaminska, J, Kaminskyy, VO, Kanamori, H, Kanasaki, K, Kang, C, Kang, R, Kang, SS, Kaniyappan, S, Kanki, T, Kanneganti, TD, Kanthasamy, AG, Kanthasamy, A, Kantorow, M, Kapuy, O, Karamouzis, MV, Karim, MR, Karmakar, P, Katare, RG, Kato, M, Kaufmann, SHE, Kauppinen, A, Kaushal, GP, Kaushik, S, Kawasaki, K, Kazan, K, Ke, PY, Keating, DJ, Keber, U, Kehrl, JH, Keller, KE, Keller, CW, Kemper, JK, Kenific, CM, Kepp, O, Kermorgant, S, Kern, A, Ketteler, R, Keulers, TG, Khalfin, B, Khalil, H, Khambu, B, Khan, SY, Khandelwal, VKM, Khandia, R, Kho, W, Khobrekar, NV, Khuansuwan, S, Khundadze, M, Killackey, SA, Kim, D, Kim, DR, Kim, DH, Kim, DE, Kim, EY, Kim, EK, Kim, H, Kim, HS, Kim, HR, Kim, JH, Kim, JK, Kim, J, Kim, KI, Kim, PK, Kim, SJ, Kimball, SR, Kimchi, A, Kimmelman, AC, Kimura, T, King, MA, Kinghorn, KJ, Kinsey, CG, Kirkin, V, Kirshenbaum, LA, Kiselev, SL, Kishi, S, Kitamoto, K, Kitaoka, Y, Kitazato, K, Kitsis, RN, Kittler, JT, Kjaerulff, O, Klein, PS, Klopstock, T, Klucken, J, Knovelsrud, H, Knorr, RL, Ko, BB, Ko, F, Ko, JL, Kobayashi, H, Kobayashi, S, Koch, I, Koch, JC, Koenig, U, Kogel, D, Koh, YH, Koike, M, Kohlwein, SD, Kocaturk, NM, Komatsu, M, Konig, J, Kono, T, Kopp, BT, Korcsmaros, T, Korkmaz, G, Korolchuk, VI, Korsnes, MS, Koskela, A, Kota, J, Kotake, Y, Kotler, ML, Kou, YJ, Koukourakis, MI, Koustas, E, Kovacs, AL, Kovacs, T, Koya, D, Kozako, T, Kraft, C, Krainc, D, Kramer, H, Krasnodembskaya, AD, Kretz-Remy, C, Kroemer, G, Ktistakis, NT, Kuchitsu, K, Kuenen, S, Kuerschner, L, Kukar, T, Kumar, A, Kumar, D, Kumar, S, Kume, S, Kumsta, C, Kundu, CN, Kundu, M, Kunnumakkara, AB, Kurgan, L, Kutateladze, TG, Kutlu, O, Kwak, S, Kwon, HJ, Kwon, TK, Kwon, YT, Kyrmizi, I, La Spada, A, Labonte, P, Ladoire, S, Laface, I, Lafont, F, Lagace, DC, Lahiri, V, Lai, ZB, Laird, AS, Lakkaraju, A, Lamark, T, Lan, SH, Landajuela, A, Lane, DJR, Lane, JD, Lang, CH, Lange, C, Langer, R, Lapaquette, P, Laporte, J, LaRusso, NF, Lastres-Becker, I, Lau, WCY, Laurie, GW, Lavandero, S, Law, BYK, Law, HKW, Layfield, R, Le, WD, Le Stunff, H, Leary, AY, Lebrun, JJ, Leck, 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Tchetina, E, Tee, AR, Tegeder, I, Teis, D, Teixeira, N, Teixeira-Clerc, F, Tekirdag, KA, Tencomnao, T, Tenreiro, S, Tepikin, AV, Testillano, PS, Tettamanti, G, Tharaux, P, Thedieck, K, Thekkinghat, AA, Thellung, S, Thinwa, JW, Thirumalaikumar, VP, Thomas, SM, Thomes, PG, Thorburn, A, Thukral, L, Thum, T, Thumm, M, Tian, L, Tichy, A, Till, A, Timmerman, V, Titorenko, VI, Todi, SV, Todorova, K, Toivonen, JM, Tomaipitinca, L, Tomar, D, Tomas-Zapico, C, Tong, BCK, Tong, C, Tong, X, Tooze, SA, Torgersen, ML, Torii, S, Torres-Lopez, L, Torriglia, A, Towers, CG, Towns, R, Toyokuni, S, Trajkovic, V, Tramontano, D, Tran, Q, Travassos, LH, Trelford, CB, Tremel, S, Trougakos, IP, Tsao, BP, Tschan, MP, Tse, HF, Tse, TF, Tsugawa, H, Tsvetkov, AS, Tumbarello, DA, Tumtas, Y, Tunon, MJ, Turcotte, S, Turk, B, Turk, V, Turner, BJ, Tuxworth, RI, Tyler, JK, Tyutereva, EV, Uchiyama, Y, UgunKlusek, A, Uhlig, HH, Ulasov, IV, Umekawa, M, Ungermann, C, Unno, R, Urbe, S, Uribe-Carretero, E, Ustun, S, Uversky, VN, Vaccari, T, Vaccaro, MI, Vahsen, BF, Vakifahmetoglu-Norberg, H, Valdor, R, Valente, MJ, Valko, A, Vallee, RB, Valverde, AM, Van den Berghe, G, van Der Veen, S, Van Kaer, L, van Loosdregt, J, van Wijk, SJL, Vandenberghe, W, Vanhorebeek, I, Vannier-Santos, MA, Vannini, N, Vanrell, MC, Vantaggiato, C, Varano, G, Varela-Nieto, I, Varga, M, Vasconcelos, MH, Vats, S, Vavvas, DG, VegaNaredo, I, Vega-Rubin-de-Celis, S, Velasco, G, Velazquez, AP, Vellai, T, Vellenga, E, Velotti, F, Verdier, M, Verginis, P, Vergne, I, Verkade, P, Verma, M, Verstreken, P, Vervliet, T, Vervoorts, J, Vessoni, AT, Victor, VM, Vidal, M, Vidoni, C, Vieira, OV, Vierstra, RD, Vigano, S, Vihinen, H, Vijayan, V, Vila, M, Vilar, M, Villalba, JM, Villalobo, A, Villarejo-Zori, B, Villarroya, F, Villarroya, J, Vincent, O, Vindis, C, Viret, C, Viscomi, MT, Visnjic, D, Vitale, I, Vocadlo, DJ, Voitsekhovskaja, OV, Volonte, C, Volta, M, Vomero, M, Von Haefen, C, Vooijs, MA, Voos, W, Vucicevic, L, Wade-Martins, R, Waguri, S, Waite, KA, Wakatsuki, S, Walker, DW, Walker, MJ, Walker, SA, Walter, J, Wandosell, FG, Wang, B, Wang, CY, Wang, C, Wang, CR, Wang, CW, Wang, D, Wang, FY, Wang, F, Wang, FM, Wang, GS, Wang, H, Wang, HX, Wang, HG, Wang, JR, Wang, JG, Wang, J, Wang, JD, Wang, K, Wang, LR, Wang, LM, Wang, MH, Wang, MQ, Wang, NB, Wang, PW, Wang, PP, Wang, P, Wang, QJ, Wang, Q, Wang, QK, Wang, QA, Wang, WT, Wang, WY, Wang, XN, Wang, XJ, Wang, Y, Wang, YC, Wang, YZ, Wang, YY, Wang, YH, Wang, YP, Wang, YQ, Wang, Z, Wang, ZY, Wang, ZG, Warnes, G, Warnsmann, V, Watada, H, Watanabe, E, Watchon, M, Weaver, TE, Wegrzyn, G, Wehman, AM, Wei, HF, Wei, L, Wei, TT, Wei, YJ, Weiergraber, OH, Weihl, CC, Weindl, G, Weiskirchen, R, Wells, A, Wen, RXH, Wen, X, Werner, A, Weykopf, B, Wheatley, SP, Whitton, JL, Whitworth, AJ, Wiktorska, K, Wildenberg, ME, Wileman, T, Wilkinson, S, Willbold, D, Williams, B, Williams, RSB, Williams, RL, Williamson, PR, Wilson, RA, Winner, B, Winsor, NJ, Witkin, SS, Wodrich, H, Woehlbier, U, Wollert, T, Wong, E, Wong, JH, Wong, RW, Wong, VKW, Wong, WWL, Wu, AG, Wu, CB, Wu, J, Wu, JF, Wu, KK, Wu, M, Wu, SY, Wu, SZ, Wu, SF, Wu, WKK, Wu, XH, Wu, XQ, Wu, YW, Wu, YH, Xavier, RJ, Xia, HG, Xia, LX, Xia, ZY, Xiang, G, Xiang, J, Xiang, ML, Xiang, W, Xiao, B, Xiao, GZ, Xiao, HY, Xiao, HT, Xiao, J, Xiao, L, Xiao, S, Xiao, Y, Xie, BM, Xie, CM, Xie, M, Xie, YX, Xie, ZP, Xie, ZL, Xilouri, M, Xu, CF, Xu, E, Xu, HX, Xu, J, Xu, JR, Xu, L, Xu, WW, Xu, XL, Xue, Y, Yakhine-Diop, SMS, Yamaguchi, M, Yamaguchi, O, Yamamoto, A, Yamashina, S, Yan, SM, Yan, SJ, Yan, Z, Yanagi, Y, Yang, CB, Yang, DS, Yang, H, Yang, HT, Yang, JM, Yang, J, Yang, JY, Yang, L, Yang, M, Yang, PM, Yang, Q, Yang, S, Yang, SF, Yang, WN, Yang, WY, Yang, XY, Yang, XS, Yang, Y, Yao, HH, Yao, SG, Yao, XQ, Yao, YG, Yao, YM, Yasui, T, Yazdankhah, M, Yen, PM, Yi, C, Yin, XM, Yin, YH, Yin, ZY, Ying, MD, Ying, Z, Yip, CK, Yiu, SPT, Yoo, YH, Yoshida, K, Yoshii, SR, Yoshimori, T, Yousefi, B, Yu, BX, Yu, HY, Yu, J, Yu, L, Yu, ML, Yu, SW, Yu, VC, Yu, WH, Yu, ZP, Yu, Z, Yuan, JY, Yuan, LQ, Yuan, SL, Yuan, SSF, Yuan, YG, Yuan, ZQ, Yue, JB, Yue, ZY, Yun, J, Yung, RL, Zacks, DN, Zaffagnini, G, Zambelli, VO, Zanella, I, Zang, QS, Zanivan, S, Zappavigna, S, Zaragoza, P, Zarbalis, KS, Zarebkohan, A, Zarrouk, A, Zeitlin, SO, Zeng, JL, Zeng, JD, Zerovnik, E, Zhan, LX, Zhang, B, Zhang, DD, Zhang, HL, Zhang, H, Zhang, HH, Zhang, HF, Zhang, HY, Zhang, JB, Zhang, JH, Zhang, JP, Zhang, KLYB, Zhang, LSW, Zhang, L, Zhang, LS, Zhang, LY, Zhang, MH, Zhang, P, Zhang, S, Zhang, W, Zhang, XN, Zhang, XW, Zhang, XL, Zhang, XY, Zhang, X, Zhang, XX, Zhang, XD, Zhang, Y, Zhang, YJ, Zhang, YD, Zhang, YM, Zhang, YY, Zhang, YC, Zhang, Z, Zhang, ZG, Zhang, ZB, Zhang, ZH, Zhang, ZY, Zhang, ZL, Zhao, HB, Zhao, L, Zhao, S, Zhao, TB, Zhao, XF, Zhao, Y, Zhao, YC, Zhao, YL, Zhao, YT, Zheng, GP, Zheng, K, Zheng, L, Zheng, SZ, Zheng, XL, Zheng, Y, Zheng, ZG, Zhivotovsky, B, Zhong, Q, Zhou, A, Zhou, B, Zhou, CF, Zhou, G, Zhou, H, Zhou, HB, Zhou, J, Zhou, JY, Zhou, KL, Zhou, RJ, Zhou, XJ, Zhou, YS, Zhou, YH, Zhou, YB, Zhou, ZY, Zhou, Z, Zhu, BL, Zhu, CL, Zhu, GQ, Zhu, HN, Zhu, HX, Zhu, H, Zhu, WG, Zhu, YP, Zhu, YS, Zhuang, HX, Zhuang, XH, Zientara-Rytter, K, Zimmermann, CM, Ziviani, E, Zoladek, T, Zong, WX, Zorov, DB, Zorzano, A, Zou, WP, Zou, Z, Zou, ZZ, Zuryn, S, Zwerschke, W, Brand-Saberi, B, Dong, XC, Kenchappa, CS, Li, ZG, Lin, Y, Oshima, S, Rong, YG, Sluimer, JC, Stallings, CL, and Tong, CK
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flux ,macroautophagy ,phagophore ,stress ,vacuole ,Autophagosome ,LC3 ,lysosome ,neurodegeneration ,cancer - Abstract
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
- Published
- 2021
7. Microstructure and mechanical properties of ZrB2–SiC ultrahigh temperature ceramic composite joint using TiZrNiCu filler metal
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Li, Z R, primary, Wang, ZZ, additional, Wu, G D, additional, and Feng, JC, additional
- Published
- 2011
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8. Microstructure and mechanical properties of ZrB2-SiC ultrahigh temperature ceramic composite joint using TiZrNiCu filler metal.
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Li, Z R, Wang, ZZ, Wu, G D, and Feng, JC
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MICROSTRUCTURE ,MECHANICAL properties of metals ,ZIRCONIUM ,SILICON carbide ,EFFECT of temperature on metals ,CERAMIC materials ,METALLIC composites ,FILLER materials - Abstract
ZrB 2 -SiC ceramic composite was brazed by using TiZrNiCu active filler metal. The microstructure and interfacial phenomena of the joints were analysed by means of SEM, energy dispersive X-ray spectroscopy and X-ray diffraction. The joining effect was evaluated by shear strength. The results showed that the reaction products of the ZrB2 -SiC ceramic composite joint were TiC, ZrC, Ti5 Si3 , Zr2 Si, Zr(s,s) and (Ti, Zr)2 (Ni, Cu), and the microstructure was separately ZrB2 -SiC/Zr(s,s)/Ti5 Si3 +Zr2 Si+TiC+ZrC+(Ti,Zr)2 (Ni,Cu)/Zr(s,s)/ZrB2 -SiC. A conceptual interface evolution model was established to explain the interface evolution mechanism. The maximum shear strength of the brazed joints was 143·5 MPa at the brazing temperature T of 920°C and the holding time T of 10 min. [ABSTRACT FROM AUTHOR]- Published
- 2011
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9. Microstructural evolution characterization of friction stirring welded AZ31 magnesium alloy
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Zhang, H., Sanbao Lin, Wu, L., and Feng, Jc
10. Mechanical Properties and ITZ Microstructure of Recycled Aggregate Concrete Using Carbonated Recycled Coarse Aggregate
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Wu, JY, Zhang, YS, Zhu, PH, Feng, JC, and Hu, K
11. [Using Therapeutic Play to Increase the Oral Administration Adherence Rate of Preschool Pediatric Inpatients].
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Li TP, Huang SL, Wu YT, Feng JC, and Yu CC
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- Humans, Child, Preschool, Administration, Oral, Inpatients, Play and Playthings, Male, Female, Medication Adherence
- Abstract
Background & Problems: Oral administration is the most common mode of medical treatment for pediatric patients. Although over 98% of the patients in the targeted pediatric unit require oral medication, the oral administration adherence rate in 2022 was 43.9%. The reasons for this low rate were identified as: (1) no relevant oral administration educational materials, (2) no relevant continued nursing education provided, and (3) lack of assistive tools and feeding aids to help administer oral medication to young children., Purpose: This project was developed to increase the adherence rate of oral administration of preschool pediatric inpatients in our hospital., Resolutions: The improvement strategies included the design of patient-centered oral medication care guidance leaflets and QR code (quick response code) video links, the development of therapeutic play aids, adding cartoon characters to medicine feeders, and the creation of a "Rescue the Forest" picture book and video and reward stickers., Results: The oral medication adherence rate increased from 43.9% pretest to 91.9% posttest., Conclusions: This project and its positive effect on the rate of oral medication adherence may be referenced by other pediatric units.
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- 2025
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12. Effects of Different Types of Microplastics on Cold Seep Microbial Diversity and Function.
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Wu XN, Feng JC, Chen X, Lin YL, Huang Y, Zhong S, Li CR, Zhu MZ, and Zhang S
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- Ecosystem, Geologic Sediments microbiology, Water Pollutants, Chemical, Biodiversity, Microplastics toxicity, Seawater microbiology, Seawater chemistry
- Abstract
The massive production and widespread use of plastics have resulted in a growing marine plastic pollution problem. Cold seep ecosystems are maintained by microorganisms related to nitrogen and carbon cycling that occur in deep-sea areas, where cold hydrocarbon-rich water seeps from the ocean floor. Little is known about plastic pollution in this ecosystem. To fill this knowledge gap, we collected sediment and seawater samples from the Haima cold seep and conducted laboratory cultivation experiments, simulating in situ environmental conditions. Environmental factors and microbial genetics were analyzed at different stages over a 2-month cultivation period. Our main conclusions are as follows: (1) When microplastics (MPs) were added to sediment and seawater environments, the microbial communities most closely resembled those of the original habitat. The changes in the plastisphere communities were mainly associated with the culture time. (2) The co-occurrence network of the plastisphere was more fragile than that of environments. (3) Multiple environmental factors determined the community composition, whereas a small number of environmental variables drove the community function. MPs affected nitrogen cycling and methane metabolism and might aggregate pathogenic species. This work provides a better perspective of the effect of MPs on the community structure and function in cold seeps.
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- 2025
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13. Methane seepage leads to a specific microplastic aging process in the simulated cold seep environment.
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Chen X, Wu XN, Feng JC, Wang B, Li CR, Lin YL, Huang YJ, Zhong S, Zhang XC, Hu JL, and Zhang S
- Abstract
Marine microplastics pose a significant threat to ecosystems, and deep-sea regions serve as critical sinks for these pollutants. Among these regions, cold seeps harbor relatively high concentrations of microplastics. However, research on the aging of microplastics under low-temperature, dark, methane-abundant, and high-pressure conditions remains limited. Seawater and sediment were collected from various Haima cold seepage sites to simulate seepage environments in 200-mL high-pressure reactors. Four types of microplastics at high concentrations (approximately 10 %) were cultured and monitored over two months to explore how they aged. The key findings are as follows: (1) Compared to areas of weak seepage, methane seepage accelerated microplastic aging, as evidenced by increased surface roughness, enhanced C-O and (CO)-O bond formation, increased microbial colonization, and reduced contact angles. (2) Microplastic aging is more pronounced in sediments than in seawater, with biodegradable polylactic acid (PLA) exhibiting the most significant aging characteristics and carbon contribution. (3) Aged microplastics induce greater disturbances in inorganic nutrient levels than in organic matter, impacting nitrogen cycle processes involving nitrate, nitrite, and ammonium. This study results reveal the fundamental aging characteristics of microplastics in extremely deep seas and highlight their potential ecological effects., Competing Interests: Declaration of Competing Interest We declare that we have no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2025
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14. Hydrate formation in porous media with upward-migrating methane and its implications for the evolution of deep-sea cold seep ecosystems.
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Wang B, Chen X, Xie Y, Wang P, Feng JC, and Zhang S
- Abstract
Methane leaking from the deep seabed is a primary source of carbon and energy for various microorganisms, sustaining the evolution and productivity of cold seep ecosystems. However, the dynamics of methane hydrate formation under methane seepage conditions and potential impacts on the evolution of cold seep ecosystems remain unclear. This study investigated the dynamic formation characteristics of gas hydrates within cold seep sediments by simulating the methane leakage process. Using magnetic resonance imaging (MRI) to monitor the methane hydrate formation process by detecting the distribution of
1 H in pore water, we aimed to determine the influence of various parameters on hydrate formation. Our experimental results demonstrated that high flow rates and pressures combined with low temperatures would accelerate the formation of methane hydrates and effectively promote the conversion of leaked methane into solid hydrates. Our findings suggest that methane hydrate formation within cold seep sediments may regulate the availability and flux of methane for microbial activities within cold seep environments, thus influencing local biogeochemical processes and ecosystem dynamics. This study advances our understanding of the transformation of leaked methane into solid hydrate within cold seep sediments, highlighting its importance in the dynamic evolution of cold seep ecosystems and its impact on the oceanic carbon cycle., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)- Published
- 2025
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15. Shellfish and algae: Climate change mitigation through green and valuable foods.
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Zhang X, Feng JC, Le C, Chen X, and Diao Y
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- Aquaculture, Climate Change, Shellfish, Carbon Sequestration
- Abstract
Food safety issues have become increasingly severe, while the global population continues to grow. Shellfish and algae (SA), as sustainable and environmentally friendly foods, have great significance in helping countries worldwide achieve the goal of carbon neutrality. However, there is still a gap in quantitatively evaluating the carbon neutrality of SA farming. To address this gap, this study evaluated global SA farming, including aspects of carbon sinks, the drivers of carbon removal, value-added ecosystem services, and the performance of carbon reduction mechanisms. Results revealed that (1) 16.57 Mt. CO
2 -eq/yr were sequestered worldwide via SA farming during 2000-2020, wherein Asia was the dominant contributor to SA carbon sinks due to the abundance of production. (2) Product values were the main drivers for carbon sinks via SA farming. (3) The annual added value of SA was 501.40 million dollars during 2000-2020, of which algae farming accounted for the highest proportion. Mussel and laver were promising due to their excellent ability for carbon removal and added value. (4) It is estimated that SA farming can reduce carbon within a range from 47.72 Mt. CO2 -eq/yr to 1539.95 Mt. CO2 -eq/yr in 2021-2035. In general, the farming of SA is a promising way to respond to the call for carbon neutrality and food safety assurance worldwide, while the preference for mussel and laver can be further explored., Competing Interests: Declaration of competing interest We declare that we have no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2025
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16. The role of intestinal flora in metabolic dysfunction-associated steatotic liver disease and treatment strategies.
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Wang LJ, Sun JG, Chen SC, Sun YL, Zheng Y, and Feng JC
- Abstract
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a common multi-factorial liver disease, and its incidence is gradually increasing worldwide. Many reports have revealed that intestinal flora plays a crucial role for the occurrence and development of MASLD, through mechanisms such as flora translocation, endogenous ethanol production, dysregulation of choline metabolism and bile acid, and endotoxemia. Here, we review the relationship between intestinal flora and MASLD, as well as interventions for MASLD, such as prebiotics, probiotics, synbiotics, and intestinal flora transplantation. Intervention strategies targeting the intestinal flora along with its metabolites may be new targets for preventing and treating MASLD., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Wang, Sun, Chen, Sun, Zheng and Feng.)
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- 2025
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17. Methane emissions from the riverine sandy wetlands on the Mongolia Plateau.
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Li A, Luo ZL, Machacova K, Zhao S, Feng JC, Han XG, and Wang ZP
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- Mongolia, Wetlands, Methane analysis, Environmental Monitoring, Air Pollutants analysis
- Abstract
Methane (CH
4 ) processes and fluxes have been widely investigated in low-latitude tropical wetlands and high-latitude boreal peatlands. In the mid-latitude Mongolia Plateau, however, CH4 processes and fluxes have been less studied, particularly in riverine wetlands. In this study, in situ experiments were conducted in the riverine sandy wetlands of the Mongolia Plateau to gain a better understanding of CH4 emissions and their influencing mechanisms. Annual CH4 emissions were observed at 8.7 mg m-2 h-1 from the flowing water wetlands during November 2019 - October 2021, approximately 80% and 20% of which were emitted during the growing and non-growing seasons, respectively. In particular, CH4 emissions during the thawing period contributed < 5% to the annual total, contrary to the traditional idea that thawing plays an important role in annual CH4 emissions in boreal peatlands. CH4 emissions were significantly higher in the wetlands dominated by plant species than in that dominated by water body during the growing seasons; therefore, plant-mediated CH4 transport was explained as a favorable pathway for CH4 emissions from sandy soils to the atmosphere. Gene sequencing revealed differences in the phylogenies and taxonomies of methanogenic archaea and methanotrophs between the flowing and static water wetlands, suggesting that flowing water should bring oxygen and nutrients to microbial habitats and potentially affect the production, oxidation, and diffusion of CH4 in sandy wetlands., Competing Interests: Declarations. Ethics approval and consent to participate: All authors have read, understood, and complied as applicable with the statement on “Ethical responsibilities of Authors” as found in the Instructions for Authors. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)- Published
- 2024
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18. Occupational Radiation Exposure During Intraoperative 3-Dimensional Fluoroscopy in Pelvis and Acetabular Surgery.
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Gosselin MM, Ngo DH, Levack AE, Kutzler MA, Osunbayo O, Feng JC, Gary JL, Warner SJ, and Routt MLC
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- Humans, Fluoroscopy, Prospective Studies, Male, Female, Middle Aged, Adult, Pelvic Bones diagnostic imaging, Pelvic Bones injuries, Pelvic Bones surgery, Radiation Dosage, Operating Rooms, Orthopedic Procedures, Pelvis diagnostic imaging, Pelvis surgery, Occupational Exposure prevention & control, Acetabulum surgery, Acetabulum diagnostic imaging, Radiation Exposure prevention & control, Imaging, Three-Dimensional
- Abstract
Objectives: To quantify the occupational radiation exposure with a 3-dimensional (3D) fluoroscopic machine during routine use in pelvic and acetabular surgery and to determine whether the additional radiation exposure encountered with the 3D fluoroscopic spin is within previously accepted limits., Design: Prospective cohort study., Setting: Level I trauma center., Patient Selection Criteria: All patients undergoing 3D fluoroscopy intraoperatively during pelvis (OTA/AO 61B,C) or acetabular (OTA/AO 62A-C) surgery between April 2021 and July 2021., Outcome Measures and Comparisons: Radiation dose at standardized locations around the operating room during the spin portion of the 3D fluoroscopy., Results: Seventy-six 3D spins were performed on 69 patients during the study period. The average emitted radiation dose from the machine for the routine fluoroscopy portion of the case was 74.5 mGy. The average displayed radiation dose in the air for the spin portion of the case was 39.9 mGy, an average of 53.6% less radiation than the routine fluoroscopy portion. For the spin portion, the average radiation exposure seen by the patient was 3.42 mGy (centered on the patient) and the average maximal exposure in the room was 0.062 mGy. Minimal radiation was detected outside the operating room doors., Conclusions: The radiation exposure encountered by operating room personnel with 3D fluoroscopy appears to be within safe occupational limits. The marginal increase in radiation exposure during pelvic and acetabular surgery should not discourage the use of 3D imaging intraoperatively., Level of Evidence: Level IV, Case Series., Competing Interests: M. M. Gosselin: Board member for AAOS, Orthopaedic Trauma Association and Society of Military Orthopaedic Surgeons; A. E. Levack: Board member for Orthopaedic Trauma Association, Grant funding from NIH and DePuy Synthes, all unrelated this work; J. L. Gary: Stryker—consulting and royalties, Smith and Nephew—consulting, SMV Scientific—stockholder, Curvafix—consulting and stock options, AONA—Board Member, JOT and JBJS—Editorial Board Member, all unrelated to this work; S. J. Warner: AAOS, AONA, OTA Committee member, Globus, Smith and Nephew, Stryker: paid presenter/speaker; M. L. C. Routt: AONA Board member, Johnson and Johnson, Stryker and Zimmer: paid presenter/speaker. The remaining authors report no conflict of interest., (Copyright © 2024 Wolters Kluwer Health, Inc. All rights reserved.)
- Published
- 2024
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19. Exploring optimal incubation conditions and characteristics of methane oxidizing organisms in deep-sea environments.
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Zhong S, Feng JC, Chen X, Huang YJ, Wu XN, and Zhang S
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- Oxidation-Reduction, Seawater microbiology, Oxidoreductases metabolism, Temperature, Sulfates metabolism, Methane metabolism, Archaea metabolism
- Abstract
Anaerobic methanotrophic archaea (ANME) play key roles in buffering the methane budget in the deep-sea environment. This study aimed to explore the optimal environmental conditions for ANME enrichment. The result showed that the sample at 10.5 MPa contained the largest copy numbers of methyl-coenzyme M reductase alpha subunit (mcrA) gene (1.1 × 10
6 copies/g) compared to any other pressures and the sample at 4 °C contained higher mcrA gene (1.6 × 106 copies/g) than other temperatures. The optimal enrichment pressure for ANME-2c is 10.5 MPa at 4 °C, with an optimal subsequent incubation for ANME-2c less than 211days. Moreover, the beta nearest taxon index was significantly correlated with the incubation time (P<0.05). Total inorganic carbon and sulfate ion were key environmental factors driving community construction. This study offers insights into how ANME-2c was enriched and how species coexist in shared habitats during enrichment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)- Published
- 2024
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20. Exploring Porous Flow Behavior of the Decomposed Gas from CH 4 Hydrate in Clayey Sediments by Molecular Dynamics Simulation.
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Yan KF, Mao MH, Kou X, Li XS, Chen ZY, Wang Y, and Feng JC
- Abstract
A fundamental understanding of the fluid flow mechanism during CH
4 hydrate dissociation in nanoscale clayey sediments from the molecular perspective can provide invaluable information for macroscale natural gas hydrate (NGH) exploration. In this work, the fluid flow behaviors of the decomposed gas from CH4 hydrate within clayey nanopores under different temperature conditions are revealed by molecular dynamics (MD) simulation. The simulation results indicate that the key influencing factors of gas-water flow in nanoscale clayey sediments include the diffusion and the random migration of gas molecules. The influencing mechanisms of fluid flow in nanopores are closely related with the temperature conditions. Under a low temperature condition, the gas diffusion process is impeded by the secondary hydrate formation, leading to the decline in gas transport velocity within nanopores. However, it is still noteworthy that the gas-water fluid flow channels are not completely blocked by the occurrence of secondary hydrate. Under a high temperature condition, the significant phenomenon of water migration during gas flow is observed, which can be ascribed to the gas-liquid entrainment effect in nanopores of the clayey sediment. These results may provide valuable implications and fundamental evidence for improving gas production efficiency in future field tests of NGH exploitation in marine sediments.- Published
- 2024
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21. Anthropogenic nitrogen pollution impacts saltmarsh resilience with inhibition of seedling establishment and population dispersal.
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Liang J, Cai Y, Zhu Z, Feng JC, Zhang S, Wan H, and Zhang X
- Subjects
- Seedlings, Nitrogen, Wetlands, Poaceae physiology, Ecosystem, Resilience, Psychological
- Abstract
Saltmarsh, a prominent buffer ecosystem, has been identified as an important sink for nitrogen (N) pollutants from marine- and land-based anthropogenic activities. However, how the enriched anthropogenic N impacts saltmarsh sustainability has been neglected due to limited understanding of marsh resilience based on seedling establishment and population dispersal under anthropogenic N inputs. This study combined mesocosm experiments and model simulations to quantify the effects of increased anthropogenic N on the seedling-based vegetation expansion of Spartina alterniflora. The results indicated that seedling survivals, growth rates, and morphological indicators were inhibited by 20.08 %, 37.14 %, and > 35.56 %, respectively, under 1.5 gN/kg anthropogenic N. The sensitivity rate of vegetation expansion was increased by 70 % with 1 gN/kg increased N concentration under the scenario of low seedling density (< 15 m/yr). These findings revealed an important unidentified weakness of the marsh development process to anthropogenic N inputs. Finally, we highlighted the importance of appropriate protection measures to control nutrient pollution in salt marshes. Our study provides new insights for enhancing the resilience and sustainability of saltmarsh ecosystems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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22. [A case of treatment for severe hip trauma].
- Author
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Feng JC, Gao MX, Chen SL, Zhang W, and Luo WY
- Subjects
- Humans, Hip Injuries therapy, Hip Injuries surgery
- Published
- 2024
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23. Nonlinear differential equations and their application to evaluating the integrated impacts of multiple parameters on the biochemical safety of drinking water.
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Chen X, Wu XN, Feng JC, Wang Y, Zhang XC, Lin YL, Wang B, and Zhang S
- Subjects
- Chlorine chemistry, Chlorine pharmacology, Disinfection methods, Biofilms, Drinking Water, Disinfectants, Water Purification methods
- Abstract
The present study aimed to narrow such gaps by applying nonlinear differential equations to biostability in drinking water. Biostability results from the integrated dynamics of nutrients and disinfectants. The linear dynamics of biostability have been well studied, while there remain knowledge gaps concerning nonlinear effects. The nonlinear effects are explained by phase plots for specific scenarios in a drinking water system, including continuous nutrient release, flush exchange with the adjacent environment, periodic pulse disinfection, and periodic biofilm development. The main conclusions are, (1) The correlations between the microbial community and nutrients go through phases of linear, nonlinear, and chaotic dynamics. Disinfection breaks the chaotic phase and returns the system to the linear phase, increasing the microbial growth potential. (2) Post-disinfection after multiple microbial peaks produced via metabolism can increase disinfection efficiency and decrease the risks associated with disinfectant byproduct risks. This can provide guidelines for optimizing the disinfection strategy, according to the long-term water safety target or a short management. Limited disinfection and ultimate disinfection may be more effective and have low chemical risk, facing longer stagnant conditions. (3) Periodic biofilm formation and biofilm detachment increase the possibility of uncertainty in the chaotic phase. For future study, nonlinear differential equation models can accordingly be applied at the molecular and ecological levels to further explore more nonlinear regulation mechanisms., Competing Interests: Declaration of competing interest We declare that we have no conflict of interest., (Copyright © 2024. Published by Elsevier Ltd.)
- Published
- 2024
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24. Incidence, Risk Factors, and Prognosis of Patients with Hepatocellular Carcinoma and Brain Metastases.
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Feng JC, He Y, Polychronidis G, Xin J, You S, and Xiong J
- Subjects
- Humans, Adult, Middle Aged, Incidence, Prognosis, Risk Factors, Carcinoma, Hepatocellular diagnosis, Liver Neoplasms diagnosis, Bone Neoplasms, Brain Neoplasms epidemiology, Brain Neoplasms secondary
- Abstract
Objective: Brain metastases significantly impact the clinical course of patients with hepatocellular carcinoma (HCC). This study aimed to examine the age-related incidence, demographics, and survival of patients with HCC and brain metastases., Methods: Data of HCC patients from 2010 to 2015 in the Surveillance, Epidemiology, and End Results (SEER) Registry were screened for the presence of brain metastases. They were stratified by age and ethnicity. Multivariable logistic and Cox regression analyses were used to identify factors associated with brain metastases and those with overall survival (OS) and liver cancer-specific survival (CSS), respectively., Results: A total of 141 HCC patients presenting with brain metastases were identified, accounting for 0.35% of all HCC patients and 2.37% of patients with metastatic disease. Among all HCC patients, the incidence rate was the highest among patients aged 30-49 years old (0.47%). Ethnicity was not associated with the presence of brain metastases at the time of HCC diagnosis. However, African-American patients presented with a significantly lower disease-specific survival [median time: 1 month; interquartile range (IQR): 0-3.0 months)]. Initial lung or bone metastasis was independently associated with an increased risk of the presence of brain metastases [odds ratio (OR): 12.62, 95% confidence interval (CI): 8.40-18.97] but was not associated with a worse OS or CSS among those with brain metastases., Conclusion: This study identified the age-related incidence and risk factors of brain metastases in HCC patients. These results may contribute to the consideration of brain screening among patients with initial metastatic HCC with lung or bone metastases, and influence the counseling of this patient population regarding their prognosis., (© 2024. Huazhong University of Science and Technology.)
- Published
- 2024
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25. Exploring carbon content variation in microplastics sequestrated from seawater to sediment in the Haima cold seep area.
- Author
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Wu XN, Feng JC, Chen X, Li CR, and Zhang S
- Abstract
In the decades since plastic has become widely used, deep-sea areas, specifically cold seeps, have developed into plastic sinks. Cold seeps contain clean energy natural gas hydrates and act as a barrier reducing methane migration to the upper water column. However, the impacts of microplastics (MPs) on the carbon content in the cold seep remain unclear. In this study, we explored spatial changes in the MPs' carbon content (MPC) selecting the Haima cold seep (HCS) as the study area. The main conclusions are as follows: (1) For active seepage areas, the mass abundance of the MPs increases with the methane seepage strength in all water columns and sediment of strong seepage areas. It decreases with the seepage strength in the sediment cores in other areas. (2)The MPC is positively correlated with the depth of the water column in the non-seepage area, while it is negatively correlated in the sediment core. (3) The surface roughness of the MPs was greater in the middle of the water column and the sediment core at ROV1. In the high-pressure and oligotrophic cold seep, the amount and method of microbial utilization of carbon from the MPs deserve greater attention., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2024
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26. Midbrain dopamine oxidation links ubiquitination of glutathione peroxidase 4 to ferroptosis of dopaminergic neurons.
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Sun J, Lin XM, Lu DH, Wang M, Li K, Li SR, Li ZQ, Zhu CJ, Zhang ZM, Yan CY, Pan MH, Gong HB, Feng JC, Cao YF, Huang F, Sun WY, Kurihara H, Li YF, Duan WJ, Jiao GL, Zhang L, and He RR
- Published
- 2023
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27. Community assemblages and species coexistence of prokaryotes controlled by local environmental heterogeneity in a cold seep water column.
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Huang Y, Feng JC, Kong J, Sun L, Zhang M, Huang Y, Tang L, Zhang S, and Yang Z
- Subjects
- Geologic Sediments chemistry, Ecosystem, Methane chemistry, Water, Gammaproteobacteria
- Abstract
The distribution and heterogeneity characteristics of microbial communities in cold seep water columns are significant factors governing the efficiency of methane filtering and carbon turnover. However, this process is poorly understood. The diversity of vertically stratified microbial communities and the factors controlling the community assemblage process in the water column above the Haima cold seep were investigated in this study. The prokaryotic community diversities varied distinctly with vertical changes in hydrochemistry. Cyanobacteria dominated the light-transmitting layers and Proteobacteria dominated the deeper layers. With respect to microbial community assemblages and co-occurrence networks, stochastic processes were particularly important in shaping prokaryotic communities. In the shallow (≥85 m) and mesopelagic water columns (600-800 m), microbial community characteristics were affected by deterministic processes, reduced network connectivity, and modularity. Microbial community diversities and assemblage processes along a vertical profile were influenced by the vertical variations in pH, temperature, DIC, and nutrients. Stochastic processes may have facilitated the formation of complex co-occurrence networks. Briefly, the distribution of local environmental heterogeneity along the vertical dimension could drive unique microbial community assemblage and species coexistence patterns. This study provides new perspectives on how microorganisms adapt to the environment and build communities, and how species coexist in shared habitats., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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28. Tracing the Century-Long Evolution of Microplastics Deposition in a Cold Seep.
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Feng JC, Li CR, Tang L, Wu XN, Wang Y, Yang Z, Yuan W, Sun L, Hu W, and Zhang S
- Subjects
- Plastics, Microplastics, Methane metabolism, Ecosystem, Geologic Sediments
- Abstract
Microplastic (MP) pollution is one of the greatest threats to marine ecosystems. Cold seeps are characterized by methane-rich fluid seepage fueling one of the richest ecosystems on the seafloor, and there are approximately more than 900 cold seeps globally. While the long-term evolution of MPs in cold seeps remains unclear. Here, how MPs have been deposited in the Haima cold seep since the invention of plastics is demonstrated. It is found that the burial rates of MPs in the non-seepage areas significantly increased since the massive global use of plastics in the 1930s, nevertheless, the burial rates and abundance of MPs in the methane seepage areas are much lower than the non-seepage area of the cold seep, suggesting the degradation potential of MPs in cold seeps. More MP-degrading microorganism populations and functional genes are discovered in methane seepage areas to support this discovery. It is further investigated that the upwelling fluid seepage facilitated the fragmentation and degradation behaviors of MPs. Risk assessment indicated that long-term transport and transformation of MPs in the deeper sediments can reduce the potential environmental and ecological risks. The findings illuminated the need to determine fundamental strategies for sustainable marine plastic pollution mitigation in the natural deep-sea environments., (© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.)
- Published
- 2023
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29. Global occurrence, drivers, and environmental risks of microplastics in marine environments.
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Tang L, Feng JC, Li C, Liang J, Zhang S, and Yang Z
- Subjects
- Plastics, Environmental Monitoring, Seawater, Microplastics analysis, Water Pollutants, Chemical analysis
- Abstract
With an increasing quantity of plastic waste being discharged into the oceans, marine microplastic (MP) pollution has received widespread attention. However, the global occurrence characteristics, environmental risks, driving factors, and source-sink relationships remain unclear. In this study, we conducted a meta-analysis based on 165 articles about marine MP pollution. It was found that the global marine MP abundance displayed a significant spatial heterogeneity, and the distribution pattern was influenced by offshore distance, population density, and economic development. The morphological characteristics of MPs showed a significant difference between seawater and marine sediment, and small-size MPs (<1 mm) accounted for the majority of all MPs in the marine environment. The environmental risk assessment revealed that most of the marine MP pollution still remains at low concentrations in the global context, with the Polyurethane (PU), Polyacrylonitrile (PAN), and Polyvinyl chloride (PVC) types of MPs showing high environmental-risk contributions. In addition, land-based waste and marine operations, which were considered to be the dominant sources of marine MPs, primarily aggregated at nearshore submarine areas, in the water column, and in the deep-sea bottom environment. This study suggested that the combination of a meta-analysis and Monte Carlo simulation can provide much valuable information regarding the global occurrence characteristics and environmental risks of marine MPs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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30. Quantification and Proteomic Characterization of β-Hydroxybutyrylation Modification in the Hearts of AMPKα2 Knockout Mice.
- Author
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Ding WJ, Li XH, Tang CM, Yang XC, Sun Y, Song YP, Ling MY, Yan R, Gao HQ, Zhang WH, Yu N, Feng JC, Zhang Z, and Xing YQ
- Subjects
- Animals, Humans, Mice, Chromatography, Liquid, Mice, Inbred C57BL, Mice, Knockout, Proteomics, Tandem Mass Spectrometry, 3-Hydroxybutyric Acid chemistry, 3-Hydroxybutyric Acid metabolism, AMP-Activated Protein Kinases genetics, AMP-Activated Protein Kinases metabolism, Myocardium metabolism
- Abstract
AMP-activated protein kinase alpha 2 (AMPKα2) regulates energy metabolism, protein synthesis, and glucolipid metabolism myocardial cells. Ketone bodies produced by fatty acid β-oxidation, especially β-hydroxybutyrate, are fatty energy-supplying substances for the heart, brain, and other organs during fasting and long-term exercise. They also regulate metabolic signaling for multiple cellular functions. Lysine β-hydroxybutyrylation (Kbhb) is a β-hydroxybutyrate-mediated protein posttranslational modification. Histone Kbhb has been identified in yeast, mouse, and human cells. However, whether AMPK regulates protein Kbhb is yet unclear. Hence, the present study explored the changes in proteomics and Kbhb modification omics in the hearts of AMPKα2 knockout mice using a comprehensive quantitative proteomic analysis. Based on mass spectrometry (LC-MS/MS) analysis, the number of 1181 Kbhb modified sites in 455 proteins were quantified between AMPKα2 knockout mice and wildtype mice; 244 Kbhb sites in 142 proteins decreased or increased after AMPKα2 knockout (fold change >1.5 or <1/1.5, p < 0.05). The regulation of Kbhb sites in 26 key enzymes of fatty acid degradation and tricarboxylic acid cycle was noted in AMPKα2 knockout mouse cardiomyocytes. These findings, for the first time, identified proteomic features and Kbhb modification of cardiomyocytes after AMPKα2 knockout, suggesting that AMPKα2 regulates energy metabolism by modifying protein Kbhb., Competing Interests: Conflict of interest The authors declare no competing interests., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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31. In Situ Encapsulated Moiré Perovskite for Stable Photodetectors with Ultrahigh Polarization Sensitivity.
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Li SX, Xia H, Liu TY, Zhu H, Feng JC, An Y, Zhang XL, and Sun HB
- Abstract
Nanostructures provide a simple, effective, and low-cost route to enhance the light-trapping capability of optoelectronic devices. In recent years, nano-optical structures have been widely used in perovskite optoelectronic devices to greatly enhance the device performance. However, the inherent instability of perovskite materials hinders the practical application of these nanostructured optoelectronic devices. Here, in situ encapsulated moiré lattice perovskite photodetectors (PDs) by two nanograting-structured soft templates with relative rotation angles is fabricated. The confinement growth of the two nanograting templates leads to crystal growth with moiré lattice structure, which improves the light-harvesting ability of the perovskite crystal, thereby improving the device performance. The PD exhibits responsivity to 1026.5 A W
-1 . The Moiré lattice-perovskite-based PD maintained 95% of the initial performance after 223 days. After being continuously sprayed with water moist for 180 min, the performance is maintained at 95.7% of its initial level. The nanograting structure endows the device with high polarization sensitivity of Imax /Imin as high as 9.1., (© 2022 Wiley-VCH GmbH.)- Published
- 2023
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32. Impacts of hydraulic conditions on microplastics biofilm development, shear stresses distribution, and microbial community structures in drinking water distribution pipes.
- Author
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Chen X, Lian XY, Wang Y, Chen S, Sun YR, Tao GL, Tan QW, and Feng JC
- Subjects
- Humans, Microplastics, Plastics, Biofilms, Drinking Water, Microbiota
- Abstract
Both microplastic and biofilm are contamination sources in drinking water, but their integrated impacts on water quality have been rarely studied, especially in drinking water distribution pipes with complex hydraulic conditions. This study explored the impacts of hydraulic conditions (0-2 m/s) on microplastic biofilm (MP-BM) development, shear stresses distribution, and microbial community structures. The research was conducted for two weeks using a pilot test device to simulate practical water pipes. The following were the primary conclusions: (1) According to morphology analysis, clusters (>5 μm) significantly increased in the plastisphere when the flow velocity ranged from 0.55 m/s to 0.95 m/s, and average size of clusters decreased when the flow velocity ranged from 1.14 m/s to 1.40 m/s (2) Characteristics of MP-BM impact shear stress on both plastisphere and pipe wall biofilm. Shear stresses were positively correlated with flow velocity, number of MP-BM, and size of MP-BM, while negatively correlated with diameters of pipes. (3) 31 genera changed strictly and monotonously with the fluid velocity, accounting for 15.42%. Opportunistic pathogens in MP-BM such as Sediminibacterium, Curvibacter, and Flavobacterium were more sensitive to hydraulic conditions. Moreover, microplastics (<100 μm) deserve more attention to avoid human ingestion and to prevent mechanical damage and bio-chemical risks., Competing Interests: Declaration of competing interest None., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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33. Application of nanoarchitectonics in moist-electric generation.
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Feng JC and Xia H
- Abstract
The consumption of energy is an important resource that cannot be ignored in modern society. Non-renewable forms of energy, such as coal, natural gas, and oil, have always been important strategic resources and are always facing a crisis of shortage. Therefore, there is an urgent need for green renewable forms of energy. As an emerging green energy source, the moist-electric generator (MEG) has been studied in recent years and may become an energy source that can be utilized in daily life. Along with the advancement of technological means, nanoarchitectonics play an important role in MEG devices. This review aims to provide a comprehensive summary of the fundamentals of the MEG from the perspective of different material classifications and to provide guidance for future work in the field of MEGs. The effects of various parameters and structural designs on the output power, recent important literature and works, the mechanism of liquid-solid interactions at the nanoscale, and the application status and further potential of MEG devices are discussed in this review. It is expected that this review may provide valuable knowledge for future MEG research., (Copyright © 2022, Feng and Xia.)
- Published
- 2022
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34. Deep-sea organisms research oriented by deep-sea technologies development.
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Feng JC, Liang J, Cai Y, Zhang S, Xue J, and Yang Z
- Subjects
- Oceans and Seas, Ecosystem, Biodiversity
- Abstract
Deep-sea environment, characterized by high pressures, extremely high/low temperatures, limited photosynthesis-generated organic matter, darkness, and high levels of corrosion, is home to flourishing special ecosystems in the world. Here, we illustrate how the deep-sea equipment offers insights into the study of life in the deep sea based on the work in the past five decades. We first describe how organisms in the deep sea are studied, even though it is highly difficult to get access to such extreme environments. We then explain the role of deep-sea technologies in advancing research on the evolution of organisms in hydrothermal vents, cold seeps, seamounts, oceanic trenches, and whale falls from the following perspectives: biological diversity, mechanisms of environmental adaptation, biological evolution, and ecosystem connectivity. Finally, to better understand the function and service of deep-sea organisms, and further conserve the special creatures under anthropologic activity and climate change, we highlight the importance of innovative deep-sea technologies to promote cutting-edge research on deep-sea organisms, and note the remaining challenges and developing directions for deep-sea equipment., Competing Interests: Conflict of interest The authors declare that they have no conflict of interest., (Copyright © 2022 Science China Press. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
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35. Methane mitigation: Learning from the natural marine environment.
- Author
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Feng JC, Yan J, Wang Y, Yang Z, Zhang S, Liang S, and Li XS
- Abstract
Competing Interests: The authors declare no competing interests.
- Published
- 2022
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36. First Report of ' Candidatus Phytoplasma ziziphi' in Sweet Potato in China.
- Author
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Li QC, Chen P, Yang QQ, Chen LC, Zhang Y, Li JD, and Feng JC
- Subjects
- China, DNA, Ribosomal, RNA, Ribosomal, 16S, Ipomoea batatas, Phytoplasma genetics
- Published
- 2022
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37. In-situ Raman study on kinetics behaviors of hydrated bubble in thickening.
- Author
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Zeng XY, Wu G, Zhang S, Sun L, Sun C, Chen G, Zhong J, Li P, Yang Z, and Feng JC
- Subjects
- Kinetics, Natural Gas, Spectrum Analysis, Raman, Methane, Water
- Abstract
Natural gas leakage by means of bubbles in cold seep abundantly existed on the ocean floor, causing the change of ocean ecology and the increase of atmospheric temperature. Fortunately, hydrated bubbles as a way of methane sequestration can reduce the effect on the ocean ecology and the escape of gas bubbles from the ocean floor, and are getting attention. To know the growth mode and efficiency of gas hydrate sequestration on bubble, the thickening growth kinetics of hydrated bubble was studied in present work. In-situ Raman spectroscopy was used to analyze the evolution of gas pores and mass transfer channels in the sI CH
4 , sI CH4 -C2 H6 and sII CH4 -C2 H6 hydrate films on the hydrated bubble by the peak area ratio of Raman spectra. Three types of Raman spectra (a-, b-, and c-type), three texture structures of film (Large gas pore; Small gas pore; No gas pore) and two hydrate thickening patterns (filling of new hydrate within large gas pores; covering growth on the original hydrate lattice) were provided in the thickening of hydrated bubble. Results showed that the thickening of the hydrated bubble was a multi-stages growth, i.e., quick growth (stage I), slow growth (stage II), and no growth (stage III). The texture structures and the type and size of gas pore in hydrated bubble were critical for the kinetics growth rate of hydrated bubble in thickening. Especially, the theory of heterogeneous growth of hydrated bubble was proposed to apply the hydrate growth at the interface of two or multi- bubbles, accelerating the efficiency of carbon sequestration as the hydrated bubble. This study will provide a better theoretical basis for understanding the behaviors and efficiency of hydrated carbon sequestration on the surface of bubbles resulting from the gas leakage in the hydrate exploitation or the natural cold seep. SYNOPSIS: Hydrated bubble strongly modulates the emission of a potent greenhouse gas from the deep sea., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021. Published by Elsevier B.V.)- Published
- 2022
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38. Role of deep-sea equipment in promoting the forefront of studies on life in extreme environments.
- Author
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Liang J, Feng JC, Zhang S, Cai Y, Yang Z, Ni T, and Yang HY
- Abstract
The deep-sea environment creates the largest ecosystem in the world with the largest biological community and extensive undiscovered biodiversity. Nevertheless, these ecosystems are far from well known. Deep-sea equipment is an indispensable approach to research life in extreme environments in the deep-sea environment because of the difficulty in obtaining access to these unique habitats. This work reviewed the historical development and the state-of-the-art of deep-sea equipment suitable for researching extreme ecosystems, to clarify the role of this equipment as a promoter for the progress of life in extreme environmental studies. Linkages of the developed deep-sea equipment and the discovered species are analyzed in this study. In addition, Equipment associated with researching the deep-sea ecosystems of hydrothermal vents, cold seeps, whale falls, seamounts, and oceanic trenches are introduced and analyzed in detail. To clarify the thrust and key points of the future promotion of life in extreme environmental studies, prospects and challenges related to observing equipment, samplers, laboratory simulation systems, and submersibles are proposed. Furthermore, a blueprint for the integration of in situ observations, sampling, controllable culture, manned experiments in underwater environments, and laboratory simulations is depicted for future studies., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)
- Published
- 2021
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39. [Homology analysis and clinical infection characteristics of hypervirulent Klebsiella pneumonia ].
- Author
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Li XJ, Wang QL, Feng JC, Guan XL, Chen ZJ, and Hu B
- Subjects
- Aged, Anti-Bacterial Agents therapeutic use, Female, Humans, Male, Middle Aged, Retrospective Studies, Virulence, Klebsiella pneumoniae, Pneumonia drug therapy
- Abstract
Objective: The hvKP phenotype strains were screened from the sensitive and multi-drug resistant Klebsiella pneumoniae , and the distribution and homology of their clinical infection characteristics were compared. Methods: A total of 158 Klebsiella pneumoniae strains isolated from clinical infection specimens in the Third Affiliated Hospital of Sun Yat-sen University from 2014 to 2018 were retrospectively collected. The string test (ST) was used to screen the hvKP infection, and a total of 65 were screened. The number of patients included 51 males and 14 females, with an average age of 56 years. The composition ratio of Klebsiella pneumoniae was analyzed for clinical infection related information. Matrix assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) was used to identify the hypervirulent Klebsiella pneumoniae and establish a new database, and bio-tree cluster analysis and principal component analysis were carried out for all strains that met the experimental conditions by using analysis software and the homology of protein level was obtained. Results: A total of 65 strains hvKP were screened, 62 of which were susceptible strains and 3 were multi-drug resistant strains. The main diseases of infected patients were diagnosed as hepatobiliary disease, encephalopathy and liver abscess. The basic diseases of the population were mainly diabetes and hypertension. Dendrogram cluster analysis divided hvKP into 3 groups at a distance of 550 from the horizontal line; principal component analysis found that some strains were closely related, and the Kc group with the largest proportion was mainly related to hepatobiliary diseases. Ka group was mainly related to infection after surgery. Conclusions: HvKP was highly invasive and the clinical manifestations of infection were diverse. The infected patients were mostly elderly patients with weakened immunity. The rapid homology analysis of MALDI-TOF MS mass spectrometry has important clinical significance for the rapid screening of hvKP infection.
- Published
- 2021
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40. [Construction and Application Optimization of the Chl-a Forecast Model ARIMA for Lake Taihu].
- Author
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Li N, Li Y, Feng JC, Shan YJ, and Qian JN
- Abstract
As an important indicator of phytoplankton biomass in lakes, the chlorophyll-a (Chl-a) concentration reflects the abundance and variation of phytoplankton in the water. Based on the monthly monitoring data of Chl-a and environmental factors in Lake Taihu from December 1999 to August 2019, key environmental factors related to Chl-a and their relationships were found using the principal component analysis (PCA) method. A multiple linear stepwise regression model and an auto-regressive integrated moving average (ARIMA) model were developed to predict the monthly Chl-a concentrations. The results showed that the Chl-a concentrations in Lake Taihu exhibited clear seasonal change characteristics and an overall trend of a gradual increase. The changes in total phosphorus (TP), the permanganate index, monthly average temperature (MAT), and monthly rainfall (MR) matched the Chl-a concentrations relatively well, whereas the changes in total nitrogen (TN) and ammonium nitrogen (NH
4 + -N) lagged significantly. The PCA results showed that the increased phytoplankton biomass and consequent algae outbreaks in Lake Taihu were not limited to the effect of a single factor such as TN or TP, but were comprehensively affected by multiple factors such as TN, NH4 + -N, TP, the permanganate index, MR, and MAT. Through further validation, the ARIMA model of Chl-a concentrations was proved to be significantly better than the multiple linear stepwise regression model, especially when considering the key environmental factors as independent variables and optimizing their values. The established ARIMA (0,1,1) (0,1,1) model would be helpful for forecasting algae blooms in Lake Taihu and provide useful suggestions for water environmental management, such as water resources dispatch and regulation.- Published
- 2021
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41. Phenome-wide investigation of the causal associations between childhood BMI and adult trait outcomes: a two-sample Mendelian randomization study.
- Author
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Dong SS, Zhang K, Guo Y, Ding JM, Rong Y, Feng JC, Yao S, Hao RH, Jiang F, Chen JB, Wu H, Chen XF, and Yang TL
- Subjects
- Adult, Aged, Child, Child, Preschool, Diet, Genetic Pleiotropy, Humans, Life Style, Middle Aged, Multivariate Analysis, Pediatric Obesity genetics, Body Mass Index, Genome-Wide Association Study, Mendelian Randomization Analysis, Phenomics, Quantitative Trait, Heritable
- Abstract
Background: Childhood obesity is reported to be associated with the risk of many diseases in adulthood. However, observational studies cannot fully account for confounding factors. We aimed to systematically assess the causal associations between childhood body mass index (BMI) and various adult traits/diseases using two-sample Mendelian randomization (MR)., Methods: After data filtering, 263 adult traits genetically correlated with childhood BMI (P < 0.05) were subjected to MR analyses. Inverse-variance weighted, MR-Egger, weighted median, and weighted mode methods were used to estimate the causal effects. Multivariable MR analysis was performed to test whether the effects of childhood BMI on adult traits are independent from adult BMI., Results: We identified potential causal effects of childhood obesity on 60 adult traits (27 disease-related traits, 27 lifestyle factors, and 6 other traits). Higher childhood BMI was associated with a reduced overall health rating (β = - 0.10, 95% CI - 0.13 to - 0.07, P = 6.26 × 10
-11 ). Specifically, higher childhood BMI was associated with increased odds of coronary artery disease (OR = 1.09, 95% CI 1.06 to 1.11, P = 4.28 × 10-11 ), essential hypertension (OR = 1.12, 95% CI 1.08 to 1.16, P = 1.27 × 10-11 ), type 2 diabetes (OR = 1.36, 95% CI 1.30 to 1.43, P = 1.57 × 10-34 ), and arthrosis (OR = 1.09, 95% CI 1.06 to 1.12, P = 8.80 × 10-9 ). However, after accounting for adult BMI, the detrimental effects of childhood BMI on disease-related traits were no longer present (P > 0.05). For dietary habits, different from conventional understanding, we found that higher childhood BMI was associated with low calorie density food intake. However, this association might be specific to the UK Biobank population., Conclusions: In summary, we provided a phenome-wide view of the effects of childhood BMI on adult traits. Multivariable MR analysis suggested that the associations between childhood BMI and increased risks of diseases in adulthood are likely attributed to individuals remaining obese in later life. Therefore, ensuring that childhood obesity does not persist into later life might be useful for reducing the detrimental effects of childhood obesity on adult diseases.- Published
- 2021
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42. Emerging Role of Ferroptosis in the Pathogenesis of Ischemic Stroke: A New Therapeutic Target?
- Author
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Bu ZQ, Yu HY, Wang J, He X, Cui YR, Feng JC, and Feng J
- Subjects
- Humans, Lipid Peroxidation, Brain Ischemia complications, Brain Ischemia drug therapy, Ferroptosis, Ischemic Stroke, Stroke drug therapy
- Abstract
Ischemic stroke is one of the main causes of high morbidity, mortality, and disability worldwide; however, the treatment methods are limited and do not always achieve satisfactory results. The pathogenesis of ischemic stroke is complex, defined by multiple mechanisms; among them, programmed death of neuronal cells plays a significant role. Ferroptosis is a novel type of regulated cell death characterized by iron redistribution or accumulation and increased lipid peroxidation in the membrane. Ferroptosis is implicated in many pathological conditions, such as cancer, neurodegenerative diseases, and ischemia-reperfusion injury. In this review, we summarize current research findings on ferroptosis, including possible molecular mechanisms and therapeutic applications of ferroptosis regulators, with a focus on the involvement of ferroptosis in the pathogenesis and treatment of ischemic stroke. Understanding the role of ferroptosis in ischemic stroke will throw some light on the development of methods for diagnosis, treatment, and prevention of this devastating disease.
- Published
- 2021
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43. [Effects of water and nitrogen source types on soil enzyme activity and nitrogen utilization efficiency of wheat].
- Author
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Sun W, Liu SJ, Feng JC, Wang PT, Ma DY, Xie YX, Lu HF, and Wang CY
- Subjects
- Agricultural Irrigation, Biomass, Soil, Water analysis, Nitrogen analysis, Triticum
- Abstract
To provide basis for high-yield and high-efficiency of wheat production, with two wheat cultivars, 'Zhengmai 366' (strong gluten) and 'Bainong 207' (medium gluten), we investigated the effects of four nitrogen source types, ammonium chloride (NT
1 ), calcium nitrate (NT2 ), urea (NT3 ) and calcium ammonium nitrate (NT4 ), applied under two water treatments, no irrigation (W1 ) and irrigation at jointing and heading stages (W2 ), on soil N-supplying capacity, grain yield and nitrogen utilization efficiency. The results showed that content of soil ammonium and nitrate at flowering stage decreased with increasing soil depths. Compared with the corresponding value of 'Zhengmai 366' under W1 treatment, W2 treatment decreased the contents of soil ammonium and nitrate in the 0-60 cm layer, and enzymes activities of urease, invertase and catalase by 10.0%, 13.3%, 7.5%, 2.8%, and 3.9%, respectively. For the two wheat cultivars, the content of ammo-nium was significantly higher under NT1 and NT3 treatments than that of others, while the content of nitrate under NT2 and NT3 treatments was significantly higher than that of others. Additionally, NT3 and NT4 treatments increased soil urease and invertase activities at the middle and later stages of grain filling. Compared with NT1 treatment, NT3 and NT4 fertilization increased grain yield and nitrogen use efficiency of cultivar 'Zhengmai 366' by 14.9% and 20.7%, 25.6% and 13.9%, under W2 treatment, respectively. Soil nitrate content in the 0-20 cm layer and the ammonium content in the 20-40 cm layer were positively correlated with wheat grain yield and nitrogen utilization efficiency. Under both water conditions, applying urea and calcium ammonium nitrate improved soil enzyme activity at the middle and later stages of grain filling, which was beneficial for wheat yield and nitrogen use efficiency.- Published
- 2020
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44. The Effects of Oral Rehmannia glutinosa Polysaccharide Administration on Immune Responses, Antioxidant Activity and Resistance Against Aeromonas hydrophila in the Common Carp, Cyprinus carpio L.
- Author
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Feng JC, Cai ZL, Zhang XP, Chen YY, Chang XL, Wang XF, Qin CB, Yan X, Ma X, Zhang JX, and Nie GX
- Subjects
- Administration, Oral, Aeromonas hydrophila immunology, Aeromonas hydrophila pathogenicity, Animals, Anti-Bacterial Agents isolation & purification, Antioxidants isolation & purification, Cell Proliferation drug effects, Cells, Cultured, Cytokines metabolism, Gram-Negative Bacterial Infections immunology, Gram-Negative Bacterial Infections metabolism, Gram-Negative Bacterial Infections microbiology, Head Kidney immunology, Head Kidney metabolism, Immunologic Factors isolation & purification, Nitric Oxide metabolism, Phagocytosis drug effects, Polysaccharides isolation & purification, Aeromonas hydrophila drug effects, Anti-Bacterial Agents administration & dosage, Antioxidants administration & dosage, Carps immunology, Carps metabolism, Carps microbiology, Gram-Negative Bacterial Infections prevention & control, Head Kidney drug effects, Immunologic Factors administration & dosage, Oxidative Stress drug effects, Polysaccharides administration & dosage, Rehmannia chemistry
- Abstract
The effects of the oral administration of Rehmannia glutinosa polysaccharide (RGP-1) on the immunoregulatory properties, antioxidant activity, and resistance against Aeromonas hydrophila in Cyprinus carpio L. were investigated. The purified RGP-1 (250, 500, and 1,000 μg/mL) was co-cultured with the head kidney cells of the common carp. The proliferation and phagocytosis activities of the head kidney cells, and the concentration of nitric oxide (NO) and cytokines in the culture medium were determined. Next, 300 common carps (47.66 ± 0.43 g) were randomly divided into five groups; the two control groups (negative and positive) were administered sterile PBS and the three treatment groups were administered different concentrations of RGP-1 (250, 500, and 1,000 μg/mL) for seven days. Subsequently, the positive and treatment groups were infected with A. hydrophila , and the negative group was administered sterile PBS for 24 h. The concentration of NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in serum, the total antioxidant capacity (T-AOC), the levels of malonaldehyde (MDA) and glutathione (GSH), and the total activities of superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the hepatopancreas of the common carp were tested. We observed that RGP-1 could significantly enhance the proliferation and phagocytosis activities ( P < 0.05), besides inducing the production of NO, pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12) and anti-inflammatory cytokines (IL-10, TGF-β) ( P < 0.05) in vitro . The in vivo experimental results revealed that RGP-1 significantly enhanced NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12), LZM and AKP activities, and the antioxidant content (T-AOC, SOD, CAT, GSH, GSH-Px, and MDA) compared to that observed in the negative group prior to A. hydrophila infection ( P < 0.05). NO, pro-inflammatory cytokines, LZM and AKP activities were significantly lower than that in the positive group after infection ( P < 0.05). However, whether infected or not, the expression of anti-inflammatory cytokines (IL-10, TGF-β) increased significantly in the RGP-1-treated groups ( P < 0.05). Therefore, the results suggested that RGP-1 could enhance the non-specific immunity, antioxidant activity and anti- A. hydrophila activity of the common carp, and could be used as a safe and effective feed additive in aquaculture., (Copyright © 2020 Feng, Cai, Zhang, Chen, Chang, Wang, Qin, Yan, Ma, Zhang and Nie.)
- Published
- 2020
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45. [Effect of soil moisture on water potential gradients in the soil-plant-atmosphere continuum (SPAC) of apple orchards in the Loess Plateau, Northwest China].
- Author
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Dang HZ, Que XE, Feng JC, Wang MM, and Chen S
- Subjects
- China, Droughts, Water, Malus, Soil
- Abstract
The variations of water potential gradients through the soil-plant-atmosphere continuum (SPAC) are of great significance to reveal the responses of plant water use to environmental changes. We conducted a continuous experiment to monitor the potentials in the near-canopy atmosphere (Ψ
air ), soil (Ψsoil ) and plant xylems (Ψstem ) during the growing season in an apple orchard located in the Loess Plateau. The results showed that the average Ψstem during the growing season ranged from -0.24 to -2.0 MPa, with a mean value of -0.57 MPa. The average water potential gradient in soil-plant-atmosphere system was 1:9.8:1155 (Ψsoil :Ψstem :Ψair ). We found a significant positive linear correlation between the Ψstem :Ψsoil gradient and volumetric soil water content (VWC, %). The Ψstem was more strongly correlated with Ψsoil than Ψair . Moreover, the sensitivity of Ψstem to Ψsoil decreased when Ψsoil was lower than -0.08 MPa which corresponded to VWC=17%, 0.56 times of field capacity. This was reflected by the increased linearity between Ψair /Ψstem and Ψsoil as Ψsoil decreased. There was a threshold effect for the relationship between Ψair and Ψstem . That is, the Ψstem in a day increased with the increasing of Ψair before the latter reached -69 MPa, after which the Ψstem decreased. The decline of soil water content caused an obvious decrease in water potential gradient through the SPAC system, and the threshold effect existed when VWC was below 17%. The results provide a basis for understanding the mechanisms of plant water in response to soil and atmospheric drought.- Published
- 2020
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46. S doped NiCo 2 O 4 nanosheet arrays by Ar plasma: An efficient and bifunctional electrode for overall water splitting.
- Author
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Lin JH, Yan YT, Xu TX, Qu CQ, Li J, Cao J, Feng JC, and Qi JL
- Abstract
Transition metal oxides show great potential as electrocatalysts, owing to the low cost and rich chemical states. However, the limited surface areas, low intrinsic activity and poor hydrogen evolution reaction (HER) activity greatly restrict the application for overall water splitting. Herein, we have constructed S doped NiCo
2 O4 nanosheet arrays by Ar plasma (Ar-NiCo2 O4 |S) to enhance active sites and boost catalytic kinetics. Consequently, the Ar-NiCo2 O4 |S shows the improved performances for HER and oxygen evolution reaction (OER). Further, as bifunctional electrocatalysts, Ar-NiCo2 O4 |S exhibit a voltage of 1.63 V at 10 mA cm-2 , as well as good stability., (Copyright © 2019 Elsevier Inc. All rights reserved.)- Published
- 2020
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47. A novel CCM3 mutation associated with cerebral cavernous malformation in a Chinese family.
- Author
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Jiang XY, Zhang Y, Yin X, Nan D, Wang X, Feng JC, and Miao J
- Abstract
Background: Cerebral cavernous malformation (CCM), especially the familial form, is a relatively rare congenital and occult vascular disease of the central nervous system. The familial form of CCM has been linked to three different genes: KRIT1 / CCM1, MGC4607 / CCM2 , and PDCD10 / CCM3 ; however, the genetic basis of CCM is not well understood. The PDCD10 / CCM3 is the most recent gene to be identified that results in worse clinical symptoms. Early diagnosis and treatment is important for patient prognosis., Case Report: The proband is a 38-year-old male who has been suffering from weakness in the limbs for 7 months. Investigation of his family history revealed that his mother also suffered from limbs paralysis and had been bedridden for a long time. His older brother suffered from headache for years, whereas his younger brother was asymptomatic. Brain computed tomography analysis of all family members showed multiple high-density shadows. Subsequently, magnetic resonance imaging analysis identified more prominent and similar multiple intracranial lesions in all family members. The lesions were hypo-intense, or showed mixed signs on T1-weighted imaging, and were significantly more intense on T2-weighted imaging. To understand the genetic basis of the disease in the family, DNA sequencing analysis was performed. A novel deletion mutation in the PDCD10 / CCM3 gene was identified in the proband and his relatives. The deletion resulted in a frameshift mutation and premature termination of translation of the protein, and potentially caused the disease in this family., Conclusions: Our study identified a novel PDCD10 / CCM3 heterozygous deletion (c.165delT) associated with CCM. This finding expands the CCM gene mutation profile, which will be beneficial for genetic counseling and clinical therapy., Competing Interests: Conflict of interest statement: The authors declare that there is no conflict of interest., (© The Author(s), 2020.)
- Published
- 2020
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48. Sesquiterpenoids from Artemisia vestita and Their Antifeedant and Antifungal Activities.
- Author
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Ding YH, Wang HT, Shi S, Meng Y, Feng JC, and Wu HB
- Subjects
- Animals, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents pharmacology, Antifungal Agents chemistry, Artemisia chemistry, Fungi pathogenicity, Larva drug effects, Lepidoptera microbiology, Plant Extracts chemistry, Sesquiterpenes isolation & purification, Sesquiterpenes pharmacology, Antifungal Agents pharmacology, Fungi drug effects, Plant Extracts pharmacology, Sesquiterpenes chemistry
- Abstract
Four new sesquiterpenoids, named artemivestinolide D-G ( 1 - 4 ) and three known sesquiterpenoids ( 5 - 7 ), were isolated from Artemisia vestita. The structures of these new compounds were determined based on extensive spectroscopic data analyses. Furthermore, the electronic circular dichroism data determined the absolute configurations of the new compounds. The antifeedant and antifungal activities of the isolates were evaluated against third-instar larvae of Plutella xylostella and three plant pathogenic fungi. Compounds 1 - 7 showed moderate antifeedant activities and compounds 1 - 4 and 6 - 7 exhibited antifungal activities.
- Published
- 2019
- Full Text
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49. Mechanisms underlying the regulation of intracellular and luminal pH in vaginal epithelium.
- Author
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Zhang YL, Feng JC, Ke LJ, Xu JW, Huang ZX, Huang J, Zhu YX, and Zhou WL
- Abstract
The vagina provides a characteristic low-Na
+ and low-pH fluid microenvironment that is considered generally protective. Previous studies have shown that various types of epithelial cells harbor the capacity of intracellular pH (pHi) regulation. However, it remains elusive whether vaginal epithelium could actively regulate pHi by transporting acid-base ions. In this study, we verified that after transient exposure to NH4 Cl, the pHi values could rapidly recover from acidification via Na+ -H+ exchanger (NHE), Na+ -HCO3 - cotransporter (NBC), and carbonic anhydrase in human vaginal epithelial cell line VK2/E6E7. Positive expression of the main acid-base transporters including NHE1-2, NBCe1-2, and NBCn1 mRNA was also detected in VK2/E6E7 cells. Moreover, the in vivo study further showed that interfering with the function of V-type H+ -ATPase, NHE or NBC expressed in vagina impaired vaginal luminal pH homeostasis in rats. Taken together, our study reveals the property of pH regulation in vaginal epithelial cells, which might provide novel insights into the potential role of vaginal epithelium in the formation of the vaginal acidic microenvironment., (© 2019 Wiley Periodicals, Inc.)- Published
- 2019
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50. Constitutive expression of NtabSPL6-1 in tobacco and Arabidopsis could change the structure of leaves and promote the development of trichomes.
- Author
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Ma YQ, Li Q, Pu ZQ, Lu MX, Yao JW, Feng JC, and Xu ZQ
- Subjects
- Arabidopsis growth & development, Arabidopsis metabolism, Plant Leaves genetics, Plant Leaves growth & development, Plant Proteins metabolism, Plants, Genetically Modified genetics, Plants, Genetically Modified growth & development, Plants, Genetically Modified metabolism, Nicotiana growth & development, Nicotiana metabolism, Transcription Factors metabolism, Trichomes genetics, Trichomes growth & development, Arabidopsis genetics, Gene Expression Regulation, Plant, Plant Proteins genetics, Nicotiana genetics, Transcription Factors genetics
- Abstract
The coding sequence of NtabSPL6-1 was cloned by high-fidelity PCR with specific primers and was used in construction of a binary vector for overexpression. Wild-type Col-0 Arabidopsis plants and Qinyan95 tobacco leaves were transformed using floral dip and leaf disc methods, respectively. Phenotypic observation showed that constitutive expression of NtabSPL6-1 in Arabidopsis could promote the development of trichomes on leaf epidermis and influence the growth pattern of cauline leaves. In tobacco, ectopic expression of NtabSPL6-1 led to dwarfism of the plants and alteration of the leaf structure, accompanied by changes of the glandular trichomes in development. At the same time, the self-regulation capability of NtabSPL6-1 was determined by yeast two-hybrid system. The results indicated that SBP-C terminal domain and C terminal domain of NtabSPL6-1 possessed strong transcriptional activation ability; the intact protein, N terminal domain, and the first peptide fragment in N terminal domain possessed weak transcriptional activation ability; and the second and the third peptide fragments in N terminal domain had no transcriptional activation ability, suggesting the N terminal domain of NtabSPL6-1 could block the activity of the C terminal domain. NtabSPL6-1 may affect the resistance of plants to biotic stress factors indirectly by regulation of the trichome growth., (Copyright © 2019 Elsevier GmbH. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
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