258 results on '"Fengfeng Li"'
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2. Separate-layer balanced waterflooding development technology for thick and complex carbonate reservoirs in the Middle East
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Xinmin SONG, Yong LI, Fengfeng LI, Liping YI, Benbiao SONG, Guangya ZHU, Haiyang SU, Liang WEI, and Chao YANG
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the Middle East ,thick carbonate reservoir ,vertical heterogeneity ,concealed baffle and barrier ,sealing ,separated development units ,Petroleum refining. Petroleum products ,TP690-692.5 - Abstract
Based on the waterflooding development in carbonate reservoirs in the Middle East, in order to solve the problem of the poor development effects caused by commingled injection and production, taking the thick bioclastic limestone reservoirs of Cretaceous in Iran-Iraq as an example, this paper proposes a balanced waterflooding development technology for thick and complex carbonate reservoirs. This technology includes the fine division of development units by concealed baffles and barriers, the combination of multi well type and multi well pattern, and the construction of balanced water injection and recovery system. Thick carbonate reservoirs in Iran-Iraq are characterized by extremely vertical heterogeneity, development of multi-genesis ultra-high permeability zones, and highly concealed baffles and barriers. Based on the technologies of identification, characterization, and sealing evaluation for concealed baffles and barriers, the balanced waterflooding development technology is proposed, and three types of balanced waterflooding development modes/techniques are formed, namely, conventional stratigraphic framework, fine stratigraphic framework, and deepened stratigraphic framework. Numerical simulations show that this technology is able to realize a fine and efficient waterflooding development to recover, in a balanced manner, the reserves of thick and complex carbonate reservoirs in Iran and Iraq. The proposed technology provides a reference for the development optimization of similar reservoirs.
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- 2024
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3. An omics-based characterization of Wolfiporia cocos reveals three CYP450 members involved in the biosynthetic pathway of pachymic acid
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Heping Liu, Naliang Jing, Fengfeng Li, Keyue Wang, Jing Tang, Qin Zhao, Yipeng Zhang, Hamza Armghan Noushahi, Ran Xu, Xuekui Wang, Wenjun Zhu, Shengqiu Feng, Shaohua Shu, and Zhinan Mei
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Biology (General) ,QH301-705.5 - Abstract
Abstract Wolfiporia cocos is a medicinal mushroom used in China. It biosynthesizes pachymic acid (PA), a main therapeutic triterpene associated with therapies. Nowadays, the unknown PA biosynthesis leads to difficulties in increasing its content in W. cocos. Herein, we report sequencing, assembling, and characterization of the genome and several transcriptomes of W. cocos. Sequence mining determined candidate genes that encode lanosterol synthase, sterol O-acyltransferase, and sterol C-24 methyltransferase likely involved in the steps from lanosterol to PA. Gene cluster analysis identified four CYP450 cDNAs likely involved in the biosynthesis of PA, namely WcCYP64-1, WcCYP64-2, WcCYP52, and WcCYP_FUM15, which were subjected to both overexpression and silencing in mycelia. The overexpression of each of WcCYP64-1, WcCYP52 and WcCYP_FUM15 increased the content of PA, 16α-hydroxytrametenolic acid, eburicoic acid, and tumulosic acid, while the silencing of each gene either significantly or slightly decreased the contents of these four compounds, indicating their involvement in the PA biosynthesis. In addition, different temperatures affected the expression of these genes and the formation of PA. By contrast, the overexpression and silencing of WcCYP64-2 did not alter the formation of these compounds. Taken together, these findings determine more potential steps in the biosynthetic pathway of PA for metabolic engineering.
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- 2024
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4. Transcriptomic analyses of Pinctada fucata martensii responses under stress of titanium dioxide nanoparticles
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Fengfeng Li, Jiaen Liu, Zixin Gao, Chuangye Yang, Liwei Sun, Yongshan Liao, and Yuewen Deng
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TiO2-NPs ,Pinctada fucata martensii ,transcriptomics ,protein homeostasis ,oxidative stress ,Science ,General. Including nature conservation, geographical distribution ,QH1-199.5 - Abstract
Titanium dioxide nanoparticles (TiO2-NPs) released into the environment is becoming more prevalent due to their increased usage, marine TiO2-NPs contamination is escalating concerns in coastal areas. To understand the potential impact of TiO2-NPs on transcript changes in pearl oyster (Pinctada fucata martensii), transcriptome analysis on the gill tissues of pearl oysters was conducted after 14-day TiO2-NPs exposure and 7-day brief recovery. A total of 911 differentially expressed genes (DEGs) were identified between the control group (TC) and the experimental group (TE) exposed to 14-day TiO2-NPs. Gene ontology (GO) analyses of the DEGs demonstrated their substantial enrichments in functions related to “hydrolase activity”, “oxidoreductase activity”, and “DNA integration”. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses of the DEGs indicated enrichment in several pathways, including “ubiquitin-mediated protein hydrolysis”, “ECM-receptor interactions”, “NOD-like receptor signaling pathway”, “Toll-like receptor”, and “FOXO signaling pathway”. This suggests that exposure to TiO2-NPs intensifies oxidative stress and apoptosis in pearls oysters, leading to negative effects such as disrupted protein homeostasis, decreased biomineralization activity, reduced neuronal excitability, weakened immune response, and reduced cellular metabolism. Transcriptome analysis identified 844 DEGs between the TE and recovery group (TR), which underwent a 7-day brief recovery period. GO analyses of the DEGs demonstrated their substantial enrichments in functions related to “DNA integration”, “obsolete oxidation-reduction process”, and “proteolysis”. KEGG pathways analyses of the DEGs indicated enrichment in several pathways, including “lysine degradation”, “glycine, serine, and threonine metabolism”, and “NOD-like receptor signaling pathway”. The findings indicated that although pearl oysters showed only slight relief after 7 days of brief recovery, they continued to experience negative effects from TiO2-NP exposure. Our findings shed light on the complex responses of pearl oysters to TiO2-NPs stress and offer valuable theoretical insights into the toxicological impact of TiO2-NPs on pearl oysters.
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- 2024
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5. A novel technique for treating simple transverse patellar fractures using cannulated screws: a cadaveric and clinical study
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Feng Han, Zhenjia Zhong, Ming Zhou, Qi Chen, Yinan Liu, Yongjun Rui, and Fengfeng Li
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Transverse patellar fracture ,Cannulated screw ,Minimally invasive ,Closed reduction and fracture fixation ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Tension band wiring (TBW) has conventionally been used for the open reduction and internal fixation of the patella. However, it suffers from distinct disadvantages such as large incision, implant irritation, and need for subsequent implant removal. Here, we propose a novel technique using closed reduction and percutaneous fixation with three cannulated screws (TCS), which may be an alternative to this established conventional technique. Although some researchers have proposed alternative methods including closed reduction and cannulated screw fixation, with or without additional wires through the screws, and arthroscopic-assisted reduction and fixation, there are few studies that focus on the biomechanical stability of percutaneous fixation using only cannulated screws. Thus, the purpose of this study was to evaluate TCS versus TBW for simple transverse patellar fractures in cadaveric and patients' level, aiming to determine whether TCS show superiority over TBW in terms of biomechanical stability in a cadaveric study with benign clinical feasibility and outcomes in patients. Methods We conducted a cadaveric study with 15 knee specimens that had simple transverse patellar fractures. We used two fixation techniques: TBW (group A, n = 6) and TCS (group B, n = 9). We applied sinusoidal forces (25 N–125 N) at 1/5 Hz and 90° knee flexion to simulate knee movement. We compared the displacements at the fracture site between the two groups. We also used the same technique in a total of 23 patients and followed up them for at least 1 year. Results TCS demonstrated favourable biomechanical stability in the cadaveric study. The technique also performed excellently in terms of postoperative pain, knee function recovery, and complication rates during the follow-up period. Conclusions The technique provides a surgical treatment option with small incisions, minimal soft tissue irritation, and possibly lower removal rate of bothersome material.
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- 2023
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6. Heterogeneous Reservoir Petrophysical Property and Controlling Factors in Semi-Restricted Depositional Setting: A Case Study of Yamama Formation, X Oilfield, Middle East
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Fengfeng Li, Lei Li, Haowei Chen, Wenyu Wang, and Yang Wan
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Early Cretaceous ,Yamama Formation ,semi-restricted ,bioclastic limestone ,sedimentation ,diagenesis ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
The Early Cretaceous Yamama Formation of X oilfield, deposited in a semi-restricted setting, holds considerable oil reserves. However, the reservoir is extremely heterogeneous and is poorly studied. Integrating outcrops, cores, cast thin sections, regular or special core analysis, wireline logging data from six wells, and seismic data, this study provides an improved understanding of reservoir petrophysical characteristics and geological controlling factors including sedimentation, diagenesis, and sequence. The results showed that eight lithologies are developed in the Yamama Formation, of which packstone and wackstone are dominant. The physical properties span a wide range, with porosity mainly distributed between 10% and 25%, and the permeability mainly distributed between 0.1 mD and 1 mD. Nine types of pores are developed, with moldic pores, micropores, and skeletal pores being the most developed. The reservoir has six types of microstructures, of which the poorly sorted with mega-throat represent the best reservoir. The Yamama Formation was mainly deposited in a lagoon, along with five other facies, such as supratidal flat, patchy reef, back shoal, shoal, and open shelf. Six types of diageneses are developed, with dissolution during the penecontemporaneous stage being the most beneficial to the reservoir and cementation being the most destructive. Three sequences were recognized in the Yamama Formation. It concluded that the hydrodynamics in semi-restricted depositional setting is weak overall and does not have the potential to develop large-scale high-quality reservoirs. A wide range of bioclasts were selectively dissolved to form a large number of secondary pores. Sediments rich in Algae, Bacinella, and peloids tend to form moldic pores, skeletal pores, and intergranular pores, respectively, which are prone to be favorable reservoirs. Controlled by the coupling of sedimentation and diagenesis driven by sequence, the reservoir is extremely heterogenous.
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- 2024
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7. Recent advances in nickel‐based catalysts in eCO2RR for carbon neutrality
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Weikang Peng, Fengfeng Li, Shuyi Kong, Chenxi Guo, Haotian Wu, Jiacheng Wang, Yi Shen, Xianguang Meng, and Mingxi Zhang
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carbon energy ,carbon neutrality ,CO2 reduction ,electrocatalysis ,nickel‐based materials ,Production of electric energy or power. Powerplants. Central stations ,TK1001-1841 - Abstract
Abstract The excessive use of nonrenewable energy has brought about serious greenhouse effect. Converting CO2 into high‐value‐added chemicals is undoubtedly the best choice to solve energy problems. Due to the excellent cost‐effectiveness and dramatic catalytic performance, nickel‐based catalysts have been considered as the most promising candidates for the electrocatalytic CO2 reduction reaction (eCO2RR). In this work, the electrocatalytic reduction mechanism of CO2 over Ni‐based materials is reviewed. The strategies to improve the eCO2RR performance are emphasized. Moreover, the research on Ni‐based materials for syngas generation is briefly summarized. Finally, the prospects of nickel‐based materials in the eCO2RR are provided with the hope of improving transition‐metal‐based electrocatalysts for eCO2RR in the future.
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- 2024
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8. A novel strategy for screening mutations in the voltage-gated sodium channel gene of Aedes albopictus based on multiplex PCR-mass spectrometry minisequencing technology
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Qunzheng Mu, Xin Zhao, Fengfeng Li, Wenyu Li, Xinxin Zhou, Xinchang Lun, Yiguan Wang, Dongdong Hua, Qiyong Liu, Di Xiao, and Fengxia Meng
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Aedes albopictus ,VGSC gene ,Mutation ,Single nucleotide polymorphisms ,Multiplex polymerase chain reaction-mass spectrometry mini-sequencing ,Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The current prevention and control strategy for Aedes albopictus heavily relies on comprehensive management, such as environmental management and chemical control. However, the wide application of pyrethroids has facilitated the development of insecticide resistance, primarily via mutations in the voltage-gated sodium channel (VGSC) gene. This study aims to develop a novel strategy for detecting mutations in the VGSC gene in Ae. albopictus using multiplex PCR-mass spectrometry (MPCR-MS) minisequencing technology. Methods We established a new strategy for detecting mutations in the VGSC gene in Ae. albopictus using MPCR-MS minisequencing technology. MPCR amplification and mass probe extension (MPE) were first used, followed by single nucleotide polymorphism (SNP) typing mass spectrometry, which allows the simultaneous detection of multiple mutation sites of the VGSC gene in 96 samples of Ae. albopictus. A total of 70 wild-collected Ae. albopictus were used to evaluate the performance of the method by comparing it with other methods. Results Three target sites (1016, 1532, 1534) in the VGSC gene can be detected simultaneously by double PCR amplification combined with matrix-assisted laser desorption ionization–time-of-flight mass spectrometry, achieving a detection limit of 20 fg/μl. We applied this method to 70 wild-collected Ae. albopictus, and the obtained genotypes were consistent with the routine sequencing results, suggesting the accuracy of our method. Conclusions MPCR-MS minisequencing technology provides a sensitive and high-throughput approach to Ae. albopictus VGSC gene mutation screening. Compared with conventional sequencing, this method is economical and time-saving. It is of great value for insecticide resistance surveillance in areas with a high risk of vector-borne disease. Graphical Abstract
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- 2023
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9. Research progress of carbonate rock diagenesis
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Fengfeng Li, Yu Ye, Yichang Yu, and Rui Guo
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diagenesis ,carbonate rock ,dissolution ,dolomitization ,microbial rock ,Geology ,QE1-996.5 ,Engineering geology. Rock mechanics. Soil mechanics. Underground construction ,TA703-712 - Abstract
Diagenesis is one of the important genesis of carbonate reservoirs, and it is also an important geological factor for the evaluation and prediction of carbonate reservoirs. First, the research methods of carbonate diagenesis were summarized, and the diagenesis mechanism and latest understandings of dissolution, dolomitization, cementation, neomorphism, silicification, compaction and pressolution were analyzed, and the auxiliary diagenesis factors such as bioturbation, tectonic disruption, hydrocarbon charging and micro-organisms were summarized. The evolution of the diagenetic environment under the control of tectonic-sequence were analyzed, and five sedimentary-diagenetic combinations were classified according to the sedimentary energy and diagenetic properties. Several issues needed to be improved in diagenetic research of carbonate reservoirs, which include: the diagenesis simulation system was different from the real stratigraphic environment; Diagenesis terminology was not standardized, and diagenesis mode was idealized and simplified; Lagoon, deep water and cold water and other unconventional carbonate diagenesis were in a low research degree; The double-edged influence of diagenesis on reservoirs and the quantitative research on reservoir reforms are insufficient. The development trend of carbonate diagenesis research include: by combining the history of stratum burial, the experimental simulation method will gradually move towards the real burial environment, and the diagenesis simulation software will continue to achieve modular integration and intelligence; The diagenesis model will be more refined and targeted, and the diagenesis can achieve quantitative research on the transformation of the reservoir; Research on the diagenesis mechanism of unconventional carbonate rocks will be more complete.
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- 2023
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10. Assessment of cold exposure-induced metabolic changes in mice using untargeted metabolomics
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Linqiang Gong, Shiyuan Zhao, Xue Chu, Hui Yang, Yanan Li, Shanshan Wei, Fengfeng Li, Yazhou Zhang, Shuhui Li, and Pei Jiang
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cold exposure ,metabolite ,gas chromatography-mass spectrometry ,multivariate analysis ,main tissues ,Biology (General) ,QH301-705.5 - Abstract
Background: Cold exposure (CE) can effectively modulate adipose tissue metabolism and improve metabolic health. Although previous metabolomics studies have primarily focused on analyzing one or two samples from serum, brown adipose tissue (BAT), white adipose tissue (WAT), and liver samples, there is a significant lack of simultaneous analysis of multiple tissues regarding the metabolic changes induced by CE in mice. Therefore, our study aims to investigate the metabolic profiles of the major tissues involved.Methods: A total of 14 male C57BL/6J mice were randomly assigned to two groups: the control group (n = 7) and the CE group (n = 7). Metabolite determination was carried out using gas chromatography-mass spectrometry (GC-MS), and multivariate analysis was employed to identify metabolites exhibiting differential expression between the two groups.Results: In our study, we identified 32 discriminant metabolites in BAT, 17 in WAT, 21 in serum, 7 in the liver, 16 in the spleen, and 26 in the kidney, respectively. Among these metabolites, amino acids, fatty acids, and nucleotides emerged as the most significantly altered compounds. These metabolites were found to be associated with 12 differential metabolic pathways closely related to amino acids, fatty acids, and energy metabolism.Conclusion: Our study may provide valuable insights into the metabolic effects induced by CE, and they have the potential to inspire novel approaches for treating metabolic diseases.
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- 2023
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11. m6A reader YTHDC2 mediates NCOA4 mRNA stability affecting ferritinophagy to alleviate secondary injury after intracerebral haemorrhage
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Fengfeng Li, Fang Wang, Lei Wang, Jianhua Wang, Shanshan Wei, Junjun Meng, Yanan Li, Lei Feng, and Pei Jiang
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m6A ,YTHDC2 ,NCOA4 ,ferritinophagy ,intracerebral haemorrhage ,Genetics ,QH426-470 - Abstract
ABSTRACTOxidative stress and neuronal dysfunction caused by intracerebral haemorrhage (ICH) can lead to secondary injury. The m6A modification has been implicated in the progression of ICH. This study aimed to investigate the role of the m6A reader YTHDC2 in ICH-induced secondary injury. ICH models were established in rats using autologous blood injection, and neuronal cell models were induced with Hemin. Experiments were conducted to overexpress YTH domain containing 2 (YTHDC2) and examine its effects on neuronal dysfunction, brain injury, and neuronal ferritinophagy. RIP-qPCR and METTL3 silencing were performed to investigate the regulation of YTHDC2 on nuclear receptor coactivator 4 (NCOA4). Finally, NCOA4 overexpression was used to validate the regulatory mechanism of YTHDC2 in ICH. The study found that YTHDC2 expression was significantly downregulated in the brain tissues of ICH rats. However, YTHDC2 overexpression improved neuronal dysfunction and reduced brain water content and neuronal death after ICH. Additionally, it reduced levels of ROS, NCOA4, PTGS2, and ATG5 in the brain tissues of ICH rats, while increasing levels of FTH and FTL. YTHDC2 overexpression also decreased levels of MDA and Fe2+ in the serum, while promoting GSH synthesis. In neuronal cells, YTHDC2 overexpression alleviated Hemin-induced injury, which was reversed by Erastin. Mechanistically, YTHDC2-mediated m6A modification destabilized NCOA4 mRNA, thereby reducing ferritinophagy and alleviating secondary injury after ICH. However, the effects of YTHDC2 were counteracted by NCOA4 overexpression. Overall, YTHDC2 plays a protective role in ICH-induced secondary injury by regulating NCOA4-mediated ferritinophagy.
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- 2024
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12. Biomechanical Analysis of Latin Dancers’ Lower Limb during Normal Walking
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Xiangli Gao, Datao Xu, Fengfeng Li, Julien S. Baker, Jiao Li, and Yaodong Gu
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walking ,Latin dancer ,lower limb biomechanics ,gait pattern ,Technology ,Biology (General) ,QH301-705.5 - Abstract
Latin dance involves fundamental walking steps, integral to the dance process. While resembling daily walking, Latin dance demands higher balance levels, necessitating body adjustments by dancers. These adaptations affect dancers’ gait biomechanics, prompting our study on gait differences between Latin dancers (LDs) and non-dancers (NDs). We enlisted 21 female Latin dancers and 21 subjects based on specific criteria. Participants executed walking tasks, with an independent sample t-test for 1-dimensional statistical parameter mapping (SPM 1d) analyzing stance phase variations between LDs and NDs. Notably, significant differences in ankle and hip external rotation were evident during the 16.43–29.47% (p = 0.015) and 86.35–100% (p = 0.014) stance phase. Moreover, pronounced distinctions in rectus Achilles tendon force (ATF) (12.83–13.10%, p = 0.049; 15.89–80.19%, p < 0.001) and Patellofemoral joint contact force (PTF) (15.85–18.31%, p = 0.039; 21.14–24.71%, p = 0.030) during stance were noted between LDs (Latin dancers) and NDs (Non-dancers). The study revealed dancers’ enhanced balance attributed to external ankle rotation for dance stability, coupled with augmented Achilles tendon and patellofemoral joint strength from prolonged practice. Moreover, integrating suitable Latin dance into rehabilitation may benefit those with internal rotation gait issues.
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- 2023
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13. Anthelmintics nitazoxanide protects against experimental hyperlipidemia and hepatic steatosis in hamsters and mice
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Fengfeng Li, Man Jiang, Minghui Ma, Xuyang Chen, Yidan Zhang, Yixin Zhang, Yuanyuan Yu, Yunfeng Cui, Jiahui Chen, Hui Zhao, Zhijie Sun, and Deli Dong
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Nitazoxanide ,Tizoxanide ,Hyperlipidemia ,Hepatic steatosis ,AMPK ,Autophagy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Lipid metabolism disorders contribute to hyperlipidemia and hepatic steatosis. It is ideal to develop drugs simultaneous improving both hyperlipidemia and hepatic steatosis. Nitazoxanide is an FDA-approved oral antiprotozoal drug with excellent pharmacokinetic and safety profile. We found that nitazoxanide and its metabolite tizoxanide induced mild mitochondrial uncoupling and subsequently activated AMPK in HepG2 cells. Gavage administration of nitazoxanide inhibited high-fat diet (HFD)-induced increases of liver weight, blood and liver lipids, and ameliorated HFD-induced renal lipid accumulation in hamsters. Nitazoxanide significantly improved HFD-induced histopathologic changes of hamster livers. In the hamsters with pre-existing hyperlipidemia and hepatic steatosis, nitazoxanide also showed therapeutic effect. Gavage administration of nitazoxanide improved HFD-induced hepatic steatosis in C57BL/6J mice and western diet (WD)-induced hepatic steatosis in Apoe–/– mice. The present study suggests that repurposing nitazoxanide as a drug for hyperlipidemia and hepatic steatosis treatment is promising.
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- 2022
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14. Effects of vacuum thermal cycling, ultraviolet radiation and atomic oxygen on the mechanical properties of carbon fiber/epoxy shape memory polymer composite
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Qiao Tan, Fengfeng Li, Liwu Liu, Yanju Liu, and Jinsong Leng
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Shape memory polymer composite ,Space radiation ,Thermal cycling ,Ultraviolet radiation ,Atomic oxygen ,Mechanical property ,Polymers and polymer manufacture ,TP1080-1185 - Abstract
The use of shape memory polymer composites as novel materials for next generation space deployable structures requires the study of the effect of space environment upon their properties. The resistance of carbon fiber/epoxy shape memory polymer composite to vacuum thermal cycling, ultraviolet radiation and atomic oxygen has been evaluated separately by using ground-based simulation facilities. The thermal cycling condition has a temperature range of −100 °C to +100 °C with cycle times of 0, 15, 30, and 45. The irradiation times of ultraviolet with a wavelength range of 250 nm–400 nm are 0, 80, 160, and 240 h. The atomic oxygen radiation has a translational energy of about 5 eV and a flux of about 2 × 1015 atoms/cm2 at the sample position, and the irradiation times are 0, 33, 66, and 100 h. The shape memory polymer composite specimens are compared in terms of morphology, tensile modulus, breaking strength and elongation at break before and after irradiation. Results show that thermal cycling increases tensile modulus but decreases the breaking strength and the elongation at break of material after 45 cycles. The effect of ultraviolet radiation on mechanical properties of materials depends on the radiation dose received. Atomic oxygen can negatively but slightly affect the mechanical properties of the material.
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- 2023
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15. Transformation mechanism of muddy carbonate rock by the coupling of bioturbation and diagenesis: A case study of the Cretaceous of the Mesopotamia Basin in the Middle East
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Yu YE, Fengfeng LI, Xinmin SONG, and Rui GUO
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bioturbation ,muddy carbonate rocks ,Mesopotamia Basin ,Cretaceous ,diagenesis ,dissolution ,Petroleum refining. Petroleum products ,TP690-692.5 - Abstract
The transformation mechanism of muddy carbonate rock by the coupling of bioturbation and diagenesis was studied based on core, cast thin section and physical property data of Cretaceous strata in the Mesopotamia Basin, the Middle East. There are 3 ways of biological transformation of rocks: (1) The living creatures transformed formations mechanically to make the rocks looser and intergranular pores increase. (2) After formation, burrows were backfilled with coarse-grained debris, and then unsaturated fluid infiltrated into the burrows during the penecontemporaneous period, resulting in dissolution. (3) Chemical alteration occurred in abandoned burrows and dolomitization produced a large number of intercrystalline pores. The coupling of bioturbation and dissolution occurred mainly in the penecontemporaneous phase, and was controlled by rock type, sea level decline, burrow abundance, infillings, and water environment etc. As the burrows had better physical properties than the matrix, unsaturated fluid preferentially migrated along the burrows, leading to dissolution and expansion of the burrows first and then dissolution of the matrix. The coupling of bioturbation and dolomitization occurred mainly in the burial phase. The rich organic matter and reducing bacteria in the burrow provided material basis, reducing conditions and alkaline environment for dolomitization. The metasomatism in the penecontemporaneous period had little effect on the physical properties of the burrows. When the burrows were separated from the deposition interface, equimolar metasomatism occurred in the burrows in a closed environment, forming euhedral fine-crystalline dolomite with intercrystalline pores. The transformation degree of bioturbation to muddy carbonate reservoir depends on rock type, density, connectivity, infillings and structure of the burrows. With the increase of the carbonate mud content, the improvement to rock physical properties by bioturbation becomes more prominent. When the burrows are filled with coarse-grained debris or fine-crystalline dolomite, the greater the density, the higher the connectivity, and the lower the tortuosity of burrows, the better the physical properties of the muddy carbonate rocks are.
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- 2021
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16. Effectiveness and safety of a less-invasive MCL reconstruction technique for contracted or ossified ligaments in patients with elbow stiffness: An open-label, non-randomised, prospective, multicentre trial in China
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Wenjun Liu, Hao Xiong, Wei Wang, Shuai Chen, Fengfeng Li, Junjian Liu, Hede Yan, Jingwei Zhang, Yun Qian, and Cunyi Fan
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Elbow stiffness ,Valgus stability ,Medial collateral ligament reconstruction ,Fascia and tendon patches ,Multicentre study ,Medicine (General) ,R5-920 - Abstract
Summary: Background: The elbow joint is sensitive to trauma from accidents, sports injuries, and surgical trauma. Some patients develop ossification or contracture of the medial collateral ligament (MCL) after elbow trauma. A less invasive reconstruction of the MCL can be performed after resection of diseased MCL. The biomechanical characteristics of this technique have been demonstrated and validated. However, its clinical effectiveness and safety require further confirmation in clinical practice. Methods: This open-label, non-randomised, prospective, multicentre trial included consecutive patients with elbow stiffness from five orthopaedic centres in China. Patients willing to participate in the study, with elbow stiffness caused by traumatic injury, who had reached skeletal maturity, and who had a range of motion of
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- 2022
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17. High performance bio-supercapacitor electrodes composed of graphitized hemicellulose porous carbon spheres
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Zhili Zhang, Fengfeng Li, Jiachuan Chen, Guihua Yang, Xingxiang Ji, Zhongjian Tian, Baobin Wang, Lei Zhang, and Lucian Lucia
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hemicelluloses ,template-free ,graphitic porous carbon spheres ,electrode materials ,supercapacitors ,Biotechnology ,TP248.13-248.65 - Abstract
A template-free and one-step carbonization process was developed for fabricating graphitic porous carbon spheres (GPCSs) on hemicelluloses as the electrode material for supercapacitors. This method is green, low-energy, and less time consuming compared to the conventional two-step process (pore-forming and graphitizing). It uses K2FeO4, a mild activating agent that fulfills synchronous activation and graphitization. The GPCSs is regular spherical shape, have high nanoporosity, a large specific surface area (1,250 m2 g−1), and have a high graphitization degree. A unique structural advantage includes a rich interconnected conductive network for electron transfer that shortens the ion transport distance of the electrolyte. Remarkably, the GPCSs electrode displays outstanding electrochemical performance including high specific capacitance (262 F g−1 at 1.0 A g−1), rate capability energy (80%, 20 A g−1), and excellent cycling stability (95%, 10,000 cycles). This work represents a powerful methodology to develop sustainable and low-cost energy storage devices from hemicellulose.
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- 2022
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18. Burn-induced heterotopic ossification from incidence to therapy: key signaling pathways underlying ectopic bone formation
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Xianglin Hu, Zhengwang Sun, Fengfeng Li, Chaoyin Jiang, Wangjun Yan, and Yangbai Sun
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Burn injury ,Heterotopic ossification ,Incidence ,Risk factor ,Signaling pathway ,Mechanism ,Cytology ,QH573-671 - Abstract
Abstract Burn injury is one of the potential causes of heterotopic ossification (HO), which is a rare but debilitating condition. The incidence ranges from 3.5 to 5.6 depending on body area. Burns that cover a larger percentage of the total body surface area (TBSA), require skin graft surgeries, or necessitate pulmonary intensive care are well-researched risk factors for HO. Since burns initiate such complex pathophysiological processes with a variety of molecular signal changes, it is essential to focus on HO in the specific context of burn injury to define best practices for its treatment. There are numerous key players in the pathways of burn-induced HO, including neutrophils, monocytes, transforming growth factor-β1-expressing macrophages and the adaptive immune system. The increased inflammation associated with burn injuries is also associated with pathway activation. Neurological and calcium-related contributions are also known. Endothelial-to-mesenchymal transition (EMT) and vascularization are known to play key roles in burn-induced HO, with hypoxia-inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) as potential initiators. Currently, non-steroidal anti-inflammatory drugs (NSAIDs) and radiotherapy are effective prophylaxes for HO. Limited joint motion, ankylosis and intolerable pain caused by burn-induced HO can be effectively tackled via surgery. Effective biomarkers for monitoring burn-induced HO occurrence and bio-prophylactic and bio-therapeutic strategies should be actively developed in the future.
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- 2021
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19. Enhanced activity for aerobic oxidative of alcohols over manganese oxides stimulated with interstitial nitrogen doping
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Fei Ruan, Fengfeng Li, Zhengping Dong, Qingping Ke, Yangxin Jin, Wangcheng Zhan, Minh Ngoc Ha, and Jun Tang
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Interstitial nitrogen ,N–MnO2 ,Alcohol oxidation ,Chemical technology ,TP1-1185 ,Biochemistry ,QD415-436 - Abstract
Heteroatom-doped transition-metal oxides are potential non-noble-metal-based catalysts for the aerobic oxidation of alcohols because of the synergistic effects of their multiple active sites. However, the design of efficient heteroatom-doped transition-metal oxide catalysts is hindered by the complexity of the synergistic effects of multiple active sites. We have developed a practical strategy for fabricating nitrogen-doped MnO2 (N–MnO2), in which nitrogen is selectively doped at interstitial sites in MnO2. Use of N–MnO2 as a catalyst enabled additive-free aerobic oxidation of alcohols in > 99% yield at 30 °C. The catalytic activity of commercial MnO2 (< 1%) was negligible. Systematic studies of a series of Mn-based oxide catalysts, namely commercial MnO2, MnNxO2−x (prepared by traditional N doping by treatment with NH3), and N–MnO2 showed that N–MnO2 with abundant interstitial nitrogen dopant (1.8 mol%) had the best catalytic activity. Mechanistic studies suggested that the introduction of interstitial nitrogen as a dopant in MnO2 enhanced the adsorption and dissociation of oxygen on the N–MnO2 catalyst and promoted oxidative dehydrogenation of alcohols at interstitial nitrogen and oxygen vacancy sites. In situ infrared spectroscopy showed that C–N bonds (1161 cm−1) were formed and broken during benzyl alcohol oxidation. This confirms the importance of interstitial nitrogen in the catalytic cycle. This work provides an alternative strategy based on interstitial nitrogen doping for designing nitrogen-doped transition-metal oxides for aerobic oxidative reactions.
- Published
- 2021
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20. Characteristics of High Flow Zones and a Balanced Development Strategy of a Thick Bioclastic Limestone Reservoir in the Mishrif Formation in X Oilfield, Iraq
- Author
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Fengfeng Li, Lei Li, and Jiaheng Chen
- Subjects
the Mishrif Formation ,thick bioclastic limestone ,heterogeneous ,High Flow Zones ,unbalanced development ,Technology - Abstract
The Mishrif Formation in X Oilfield in Iraq is heterogeneous and has prominent development contradictions, and the development plan required urgent adjustment. Based on data regarding the core, cast thin sections, physical property, mercury injection experiments, and development performance, the main geological factors causing the unbalanced development of the Mishrif Formation are identified, and the corresponding development strategy is proposed. The results show that the High Flow Zones (HFZs) are the main geological factors causing unbalanced production in the thick bioclastic limestone reservoir. There are three kinds of HFZs in MA, MB1, and MB2 intervals, namely, the point shoal type, the tidal channel type, and the platform margin shoal type. All HFZs have different scales and distribution patterns. HFZs have ultra-high permeability and large permeability differences with the surrounding reservoir. During development, the oil mainly comes from HFZs, and the considerable reserves in the low permeability reservoir surrounding the HFZs are difficult to develop. The size of the pore throat of the HFZs greatly varies, and permeability is mainly dominated by the mega-pore throat (>10 μm) and the macro-pore throat (2.5~10 μm). In water flood development, the injected water rapidly advances along the mega-pore throat and the macro-pore throat, and the oil in the micro-pore or medium-pore throats are difficult to be displace. It can be concluded that the Mishrif Formation is vertically heterogeneous. The connectivity of HFZs in different intervals greatly varies. As a result, the Mishrif Formation is divided into three development units, MA, MB1, and MB2 + MC, and production wells are deployed in HFZs. The MA adopts a reverse nine-point injection-production pattern, for which the well spacing is 900 m using a vertical well, and the injection well should avoid the HFZs near the faults. The MB1 adopts an irregular five-point injection-production pattern using a vertical well, and the injection wells are deployed at the edge of the tidal channel or in the lagoon. MB2_1 deploys horizontal production wells, for which the well spacing is 900 m. Horizontal production wells, for which the well spacing is 300 m, are deployed in the lower MB2, and the lateral horizontal production wells are converted into injection wells after water breakthrough, and the horizontal wells deployed in the lower part of MC should moderately inject water.
- Published
- 2023
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21. Biomass-Based Hydrothermal Carbons for the Contaminants Removal of Wastewater: A Mini-Review
- Author
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Yuanyuan Wang, Yuan Xu, Xintian Lu, Kefeng Liu, Fengfeng Li, Bing Wang, Qiang Wang, Xv Zhang, Guihua Yang, and Jiachuan Chen
- Subjects
biomass ,hydrothermal carbon ,adsorbents ,heavy metals ,organic dyes ,adsorption removal ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The preparation of adsorbents with eco-friendly and high-efficiency characteristics is an important approach for pollutant removal, and can relieve the pressure of water shortage and environmental pollution. In recent studies, much attention has been paid to the potential of hydrothermal carbonization (HTC) from biomass, such as cellulose, hemicellulose, lignin, and agricultural waste for the preparation of adsorbents. Hereby, this paper summarizes the state of research on carbon adsorbents developed from various sources with HTC. The reaction mechanism of HTC, the different products, the modification of hydrochar to obtain activated carbon, and the treatment of heavy metal pollution and organic dyes from wastewater are reviewed. The maximum adsorption capacity of carbon from different biomass sources was also evaluated.
- Published
- 2023
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22. Endovascular implantation of iodine-125 seed strand combined and stent placement for locally advanced pancreatic ductal adenocarcinoma with vascular invasion: a prospective single-arm pilot study
- Author
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Linlin Wu, Yanbo Zhang, Zihan Zhang, Fengfeng Li, and Jianjun Luo
- Subjects
pancreatic ductal adenocarcinoma ,125i seed strand ,vascular invasion. ,Medicine - Published
- 2020
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23. Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) emissions from electric arc furnaces for steelmaking
- Author
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Qiuting Yang, Lili Yang, Jia Shen, Yuanping Yang, Minxiang Wang, Xiaoyun Liu, Xuejing Shen, Changliang Li, Jia Xu, Fengfeng Li, Da Li, Guorui Liu, and Minghui Zheng
- Subjects
Electric arc furnaces ,PCDD/Fs ,Stack gas ,Congener profile ,Emission factors ,Environmental pollution ,TD172-193.5 - Abstract
The proportion of steel produced using electric arc furnaces (EAFs) is increasing. In this study, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) concentrations and patterns in stack gas samples from three typical EAFs in steelmaking plants were determined to allow the contributions of EAFs in steelmaking plants to PCDD/Fs emissions in China to be assessed. Samples collection was accomplished by adopting the automatic isokinetic sampling technique, then the PCDD/Fs congeners concentrations were analyzed qualitatively and quantitatively by isotope dilution high-resolution gas chromatography high-resolution mass spectrometry (HRGC-HRMS). The mean PCDD/Fs mass concentrations in stack gases emitted during the scrap preheating phase of plants E1, E2, and E3 were 0.48, 385.55, and 0.34 ng Nm−3, respectively, and the total toxic equivalent (WHO2005-TEQ) concentrations were 0.05, 33.16, and 0.03 ng Nm−3, respectively. The PCDD/Fs mass concentration in stack gases emitted during the melting phase at plant E3 was 0.12 ng Nm−3 (0.02 ng WHO2005-TEQ Nm−3). The operational temperature of the bag filter of the preheating in the E2 EAF was as high as 230 °C, which is conducive to the generation of PCDD/Fs, whether the temperature in the bag filter of E1 (150 °C) and E3 (120 °C) are not. Moreover, the bag filter temperature is suggested to be best controlled to below 150 °C. The emission factors (EF) for PCDD/Fs released into the air during the scrap preheating phase for plants E1, E2, and E3 were 0.13, 52, and 0.10 μg WHO2005-TEQ t−1, respectively, and the EF for the smelting phase from plant E3 was 0.14 μg WHO2005-TEQ t−1. The emission factor in E2 is 5 times higher than the highest current UNEP toolkit factor for EAFs (10 μg TEQ/tonne steel). Therefore, an update for the UNEP toolkit emission factor for a few case plants is suggested. Several congeners, including 2,3,7,8-TeCDF, 1,2,3,4,6,7,8-HpCDF, and 1,2,3,4,6,7,8-HpCDD, were dominant during the preheating phase, while 2,3,7,8-TeCDF, 1,2,3,7,8-PeCDF, and 2,3,4,7,8-PeCDF were dominant during the smelting phase. The monitoring data from the case plants helps to better understand the PCDD/Fs emissions of EAFs using preheating process stages. However, a more complete emission inventory requires more case investigations on the PCDD/Fs emissions from representative EAFs steelmaking plants.
- Published
- 2020
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24. Simultaneous optimization of the acidified water extraction for total anthocyanin content, total phenolic content, and antioxidant activity of blue honeysuckle berries (Lonicera caerulea L.) using response surface methodology
- Author
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Fengfeng Li, Hengtian Zhao, Ruiru Xu, Xiuling Zhang, Wentao Zhang, Meiling Du, Xiaochen Liu, and Lili Fan
- Subjects
antioxidant activity ,blue honeysuckle berries (Lonicera caerulea L.) ,Box–Behnken design ,response surface methodology ,total anthocyanin content ,total phenolic content ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Abstract The purpose of this study was to optimize the total anthocyanin content (TAC), total phenolic content (TPC), and antioxidant activity of acidified water extract from blue honeysuckle berries by response surface methodology (RSM). The optimized conditions were HCl concentration of 0.35%, liquid–solid ratio of 49.42 ml/g, and extraction temperature of 41.56°C for total anthocyanin content (24.01 ± 0.37 mg/g), total phenolic content (207.03 ± 3.31 mg/g), DPPH radical scavenging activity (68.24 ± 1.13%), and ABTS radical scavenging activity (70.05 ± 0.84%). The experimental results are consistent with the predicted values. The results showed that acidified water extraction was an effective, simple, and green technique for the extraction of total anthocyanins, total phenol, and antioxidant activity from blue honeysuckle berries.
- Published
- 2019
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25. Sedimentary diagenesis of rudist shoal and its control on reservoirs: A case study of Cretaceous Mishrif Formation, H Oilfield, Iraq
- Author
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Yichang YU, Longde SUN, Xinmin SONG, Rui GUO, Xingjun GAO, Minjie LIN, Liping YI, Haiying HAN, Fengfeng LI, and Hangyu LIU
- Subjects
Petroleum refining. Petroleum products ,TP690-692.5 - Abstract
Based on the core, cast thin section, whole rock analysis, conventional physical properties and high pressure mercury intrusion test, the sedimentary diagenesis characteristics of rudist shoal in Cretaceous Mishrif Formation of H Oilfield, Iraq and its control on the reservoir were studied. The rudist shoal of the Mishrif Formation develops in the high-stand systems tract and is distributed in the high places of paleogeomorphology on the edge of platform with strong hydrodynamic force. According to the relative sea level changes, lithologic evolution and sedimentary structure characteristics of the rudist shoal, the single rudist shoal is divided into four lithologic sections: A, B, C and D, that is, low-angle cross-bedding pelletoids-rudist packstone, low-angle cross-bedding and parallel bedding arene-rudist grainstone, parallel bedding rudist gravel limestone, and horizontal bedding carbonaceous mudstone. The complete sedimentary sequence of a single rudist shoal is often disrupted. Several rudist shoals superimpose to form thick rudist shoal sediment. The single rudist shoal thickness and lithologic sections assemblage change regularly in vertical direction. The rudist shoal has the characteristics of “strong dissolution, weak cementation and strong compaction”, forming pore-type reservoir with intergranular pores, intergranular dissolved pores, mold pores, and dissolved pores. With mainly coarse pore throats larger than 5 μm, the reservoir is of medium-high porosity and high permeability. There is lithological reverse cycles inside single shoals and between single shoals, with content of mud crystals decreasing from the bottom to the top, dissolution increasing, cementation decreasing in strength, pore throats getting larger, and physical properties turning better. The rudist shoal of MB2-1 at the top of the high-stand systems tract has the largest thickness, moreover, subject to the strongest atmospheric freshwater leaching, this layer has the most significant dissolution and the largest pore throat, so it is the best reservoir of the Mishrif Formation. Key words: Iraq, Cretaceous, rudist shoal, sedimentary process, diagenetic evolution, reservoir characteristics
- Published
- 2018
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26. Functional Analysis of Sterol O-Acyltransferase Involved in the Biosynthetic Pathway of Pachymic Acid in Wolfiporia cocos
- Author
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Wenjun Zhu, Ying Liu, Jing Tang, Heping Liu, Naliang Jing, Fengfeng Li, Ran Xu, and Shaohua Shu
- Subjects
pachymic acid ,biosynthetic pathway ,WcSOAT ,genes modification ,triterpenoids ,Organic chemistry ,QD241-441 - Abstract
Pachymic acid from Wolfiporia cocos possesses important medicinal values including anti-bacterial, anti-inflammatory, anti-viral, invigorating, anti-rejection, anti-tumor, and antioxidant activities. However, little is known about the biosynthetic pathway from lanostane to pachymic acid. In particular, the associated genes in the biosynthetic pathway have not been characterized, which limits the high-efficiency obtaining and application of pachymic acid. To characterize the synthetic pathway and genes involved in pachymic acid synthesis, in this study, we identified 11 triterpenoids in W. cocos using liquid chromatography tandem mass spectrometry (LC-MS/MS), and inferred the putative biosynthetic pathway from lanostane to pachymic acid based on analyzing the chemical structure of triterpenoids and the transcriptome data. In addition, we identified a key gene in the biosynthetic pathway encoding W. cocos sterol O-acyltransferase (WcSOAT), which catalyzes tumolusic acid to pachymic acid. The results show that silence of WcSOAT gene in W. cocos strain led to reduction of pachymic acid production, whereas overexpression of this gene increased pachymic acid production, indicating that WcSOAT is involved in pachymic acid synthesis in W. cocos and the biosynthesis of W. cocos pachymic acid is closely dependent on the expression of WcSOAT gene. In summary, the biosynthetic pathway of pachymic acid and the associated genes complement our knowledge on the biosynthesis of W. cocos pachymic acid and other triterpenoids, and also provides a reference for target genes modification for exploring high-efficiency obtaining of active components.
- Published
- 2021
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27. Matrine attenuates heterotopic ossification by suppressing TGF-β induced mesenchymal stromal cell migration and osteogenic differentiation
- Author
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Dong Mao, Xiaoyun Pan, Yongjun Rui, and Fengfeng Li
- Subjects
Matrine ,Heterotopic ossification ,TGF-β ,Osteogenic differentiation ,Mesenchymal stromal cells ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Purpose of the study: Heterotopic ossification (HO) is a debilitating disease characterized by extraskeletal bone formation. Active TGF-β recruits mesenchymal stromal cells (MSCs), which contribute to trauma-induced HO. Inhibiting TGF-β induced MSC migration and osteogenic differentiation could be a promising treatment for HO. Matrine is an alkaloid from the genus Sophora that can suppress pancreatic and hepatic fibrosis by regulating TGF-β/Smad signaling. We conducted this study to evaluate the effects of matrine on HO and explore the mechanisms, we carried out this study. Materials and methods: Achilles tendon puncture was performed in C57BL/6J male mice to establish the HO model. Following treatment with matrine for 3, 6, 9, and 15 weeks, mice were sacrificed and tendons were collected. In vivo, micro-CT, hematoxylin and eosin staining, CD73 and CD90 immunofluorescence, and osteocalcin staining were used to evaluate the development of HO. In vitro, a transwell migration assay was used to evaluate MSC migration. Immunohistochemistry, immunofluorescence and western blotting were used to evaluate the TGF-β/Smad2/3 pathway. Real-time PCR was conducted to analyze the transcription of alkaline phosphatase (Alp), runt-related transcription factor-2 (Runx2), osteocalcin (Ocn), osteopontin (Opn), and type I collagen (Col1). ALP activity and alizarin red staining were used to assess MSC osteogenic differentiation. Results: In vivo, matrine significantly reduced ossification and inhibited HO progression. In vitro, matrine significantly suppressed MSC migration, ALP activity, and mineralization of MSCs. Mechanistically, matrine inhibited TGF-β induced Smad2/3 phosphorylation and transcription of Runx2, Alp, and Ocn after osteoinduction. Conclusions: Matrine inhibited HO by suppressing the migration and osteogenic differentiation of TGF-β-induced-MSCsin mice.
- Published
- 2020
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28. Inhibition of overactive TGF-β attenuates progression of heterotopic ossification in mice
- Author
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Xiao Wang, Fengfeng Li, Liang Xie, Janet Crane, Gehua Zhen, Yuji Mishina, Ruoxian Deng, Bo Gao, Hao Chen, Shen Liu, Ping Yang, Manman Gao, Manli Tu, Yiguo Wang, Mei Wan, Cunyi Fan, and Xu Cao
- Subjects
Science - Abstract
Heterotopic ossification (HO) is a painful disease of unknown etiology characterized by extraskeletal bone formation after injury. Here the authors show that TGF-β is increased in HO lesions, where it promotes the early stages of HO pathology, and demonstrate that TGF-β inhibition ameliorates HO in mice.
- Published
- 2018
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29. The Dual Effect of Ionic Liquid Pretreatment on the Eucalyptus Kraft Pulp during Oxygen Delignification Process
- Author
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Letian Qi, Jinke Liu, Jianmin Peng, Guihua Yang, Fengfeng Li, Yu Xue, and Jiachuan Chen
- Subjects
ionic liquid ,pretreatment ,oxygen delignification ,fiber protection ,Organic chemistry ,QD241-441 - Abstract
Oxygen delignification presents high efficiency but causes damage to cellulose, therefore leading to an undesired loss in pulp strength. The effect of ionic liquid pretreatment of [BMIM][HSO4] and [TEA][HSO4] on oxygen delignification of the eucalyptus kraft pulp was investigated at 10% IL loading and 10% pulp consistency, after which composition analysis, pulp and fiber characterizations, and the mechanism of lignin degradation were carried out. A possible dual effect of enhancing delignification and protecting fibers from oxidation damage occurred simultaneously. The proposed [TEA][HSO4] pretreatment facilitated lignin removal in oxygen delignification and provided fibers with improved DP, fiber length and width, and curl index, resulting in the enhanced physical strength of pulp. Particularly, its folding endurance improved by 110%. An unusual brightness reduction was identified, followed by detailed characterization on the pulps and extracted lignin with FTIR, UV, XPS, and HSQC. It was proposed that [TEA][HSO4] catalyzed the cleavage of β-O-4 bonds in lignin during the oxygen delignification, with the formation of Hibbert’s ketones and quinonoid compounds. The decomposed lignin dissolved and migrated to the fiber surface, where they facilitated the access of the oxidation agent and protected the fiber framework from oxidation damage. Therefore, it was concluded that ionic liquid pretreatment has a dual effect on oxygen delignification.
- Published
- 2021
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30. Highly Efficient Ag3PO4/g-C3N4 Z-Scheme Photocatalyst for Its Enhanced Photocatalytic Performance in Degradation of Rhodamine B and Phenol
- Author
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Mingxi Zhang, Hanxiao Du, Juan Ji, Fengfeng Li, Y. C. Lin, Chenwei Qin, Ze Zhang, and Yi Shen
- Subjects
Ag3PO4 ,g-C3N4 ,semiconductor photocatalyst ,Z-scheme mechanism ,Organic chemistry ,QD241-441 - Abstract
Ag3PO4/g-C3N4 heterojunctions, with different g-C3N4 dosages, were synthesized using an in situ deposition method, and the photocatalytic performance of g-C3N4/Ag3PO4 heterojunctions was studied under simulated sunlight conditions. The results revealed that Ag3PO4/g-C3N4 exhibited excellent photocatalytic degradation activity for rhodamine B (Rh B) and phenol under the same light conditions. When the dosage of g-C3N4 was 30%, the degradation rate of Rh B at 9 min and phenol at 30 min was found to be 99.4% and 97.3%, respectively. After five cycles of the degradation experiment for Rh B, g-C3N4/Ag3PO4 still demonstrated stable photodegradation characteristics. The significant improvement in the photocatalytic activity and stability of g-C3N4/Ag3PO4 was attributed to the rapid charge separation between g-C3N4 and Ag3PO4 during the Z-scheme charge transfer and recombination process.
- Published
- 2021
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31. Preliminary design and analysis of a cubic deployable support structure based on shape memory polymer composite
- Author
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Fengfeng Li, Liwu Liu, Xin Lan, Xiaojun Zhou, Wenfeng Bian, Yanju Liu, and Jinsong Leng
- Subjects
Shape memory polymer composites ,deployable structure ,shape recovery experiment ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
The deployable structures based on shape memory polymer composites (SMPCs) have been developed for its unique properties, such as high reliability, low-cost, lightweight, and self-deployment without complex mechanical devices compared with traditional deployable structures. In order to increase the inflatable structure system’s robustness and light the weight of it, a cubic deployable support structure based on SMPC is designed and analyzed preliminarily. The cubic deployable support structure based on SMPC consists of four dependent spatial cages, each spatial cage is composed of 12 three-longeron SMPC truss booms and end connections. The shape recovery of arc-shaped deployable laminates drive the three-longeron SMPC truss booms to unfold, thus realize the expansion of the deployable support structure. The concept and operation of the cubic deployable support structure are described in detail. A series of experiments are performed on the three-longeron deployable laminates unit and the simplified cubic deployable support structure to investigate the shape recovery behavior in the deployment process. Results indicate that the cubic deployable support structure has a high deployment-tgo-stowage volume ratio and can achieve self-deployment, package, and deploy without complex mechanical devices.
- Published
- 2016
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32. The Adaptability of APSIM-Wheat Model in the Middle and Lower Reaches of the Yangtze River Plain of China: A Case Study of Winter Wheat in Hubei Province
- Author
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Panpan Zhao, Yang Zhou, Fengfeng Li, Xiaoxia Ling, Nanyan Deng, Shaobing Peng, and Jianguo Man
- Subjects
the middle and lower reaches of the Yangtze River plain ,winter wheat ,APSIM-wheat model ,calibration and validation ,adaptability ,Agriculture - Abstract
The middle and lower reaches of the Yangtze River (MLYR) plain represent the second-largest wheat producing area in China; the winter wheat-rice system is one of the main planting systems in this region. The use of the agricultural production system simulator (APSIM)-wheat model to simulate wheat production potential and evaluate the impact of future climate change on wheat production in this region is of great importance. In this study, the adaptability of the APSIM-wheat model in the MLYR was evaluated based on observational data collected in field experiments and daily meteorological data from experimental stations in Wuhan, Jingmen, and Xiangyang in Hubei province. The results showed significant positive relationships between model-predicted wheat growth duration from sowing to anthesis and maturity and the observed values, with coefficients of determination (R2) in ranges of 0.90–0.97 and 0.93–0.96, respectively. The normalized root-mean-square error (NRMSE) of the simulated growth durations and measured values were lower than 1.6%, and the refined index of agreement (dr-values) was in the range of 0.74–0.87. The percent mean absolute relative error (PMARE) was cited here as a new index, with a value below 1.4%, indicating that the model’s rating was excellent. The model’s performance in terms of grain yield and above-ground biomass simulation was also acceptable, although it was not as good as the growth periods simulation. The R2 value was higher than 0.75 and 0.72 for the simulation of grain yield and biomass, respectively. The indices NRMSE and PMARE were lower than 19.8% and 19.9%, and the dr-value was higher than 0.71. According to our results, APSIM-wheat was an effective and accurate model for simulating the phenology and yield production processes of wheat in the MLYR, and the results also provided a theoretical basis and technical support for further research on the yield potential of wheat-rice rotation planting systems with clarification of the key factors limiting the yield gap in this region.
- Published
- 2020
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33. Nitrogen Deficiency-Induced Decrease in Cytokinins Content Promotes Rice Seminal Root Growth by Promoting Root Meristem Cell Proliferation and Cell Elongation
- Author
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Qi Wang, Yanchun Zhu, Xiao Zou, Fengfeng Li, Jialiang Zhang, Ziyi Kang, Xuefei Li, Changxi Yin, and Yongjun Lin
- Subjects
cell elongation ,cell proliferation ,cytokinin ,nitrogen deficiency ,rice ,root meristem ,Cytology ,QH573-671 - Abstract
Rice (Oryza sativa L.) seedlings grown under nitrogen (N) deficiency conditions show a foraging response characterized by increased root length. However, the mechanism underlying this developmental plasticity is still poorly understood. In this study, the mechanism by which N deficiency influences rice seminal root growth was investigated. The results demonstrated that compared with the control (1 mM N) treatment, N deficiency treatments strongly promoted seminal root growth. However, the N deficiency-induced growth was negated by the application of zeatin, which is a type of cytokinin (CK). Moreover, the promotion of rice seminal root growth was correlated with a decrease in CK content, which was due to the N deficiency-mediated inhibition of CK biosynthesis through the down-regulation of CK biosynthesis genes and an enhancement of CK degradation through the up-regulation of CK degradation genes. In addition, the N deficiency-induced decrease in CK content not only enhanced the root meristem cell proliferation rate by increasing the meristem cell number via the down-regulation of OsIAA3 and up-regulation of root-expressed OsPLTs, but also promoted root cell elongation by up-regulating cell elongation-related genes, including root-specific OsXTHs and OsEXPs. Taken together, our data suggest that an N deficiency-induced decrease in CK content promotes the seminal root growth of rice seedlings by promoting root meristem cell proliferation and cell elongation.
- Published
- 2020
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34. UPLC-QTOF-MS metabolomics analysis revealed the contributions of metabolites to the pathogenesis of Rhizoctonia solani strain AG-1-IA.
- Author
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Wenjin Hu, Xinli Pan, Fengfeng Li, and Wubei Dong
- Subjects
Medicine ,Science - Abstract
To explore the pathogenesis of Rhizoctonia solani and its phytotoxin phenylacetic acid (PAA) on maize leaves and sheaths, treated leaf and sheath tissues were analyzed and interpreted by ultra-performance liquid chromatography-mass spectrometry combined with chemometrics. The PAA treatment had similar effects to those of R. solani on maize leaves regarding the metabolism of traumatin, phytosphingosine, vitexin 2'' O-beta-D-glucoside, rutin and DIBOA-glucoside, which were up-regulated, while the synthesis of OPC-8:0 and 12-OPDA, precursors for the synthesis of jasmonic acid, a plant defense signaling molecule, was down-regulated under both treatments. However, there were also discrepancies in the influences exhibited by R. solani and PAA as the metabolic concentration of zeaxanthin diglucoside in the R. solani infected leaf group decreased. Conversely, in the PAA-treated leaf group, the synthesis of zeaxanthin diglucoside was enhanced. Moreover, although the synthesis of 12 metabolites were suppressed in both the R. solani- and PAA-treated leaf tissues, the inhibitory effect of R. solani was stronger than that of PAA. An increased expression of quercitrin and quercetin 3-O-glucoside was observed in maize sheaths treated by R. solani, while their concentrations were not changed significantly in the PAA-treated sheaths. Furthermore, a significant decrease in the concentration of L-Glutamate, which plays important roles in plant resistance to necrotrophic pathogens, only occurred in the R. solani-treated sheath tissues. The differentiated metabolite levels may be the partial reason of why maize sheaths were more susceptible to R. solani than leaves and may explain the underlying mechanisms of R. solani pathogenesis.
- Published
- 2018
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35. Ionic Liquids as a New Platform for Fiber Brittleness Removal
- Author
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Zhili Zhang, Jiachuan Chen, Zhiqiang Pang, Lucian A. Lucia, Fengfeng Li, and Guihua Yang
- Subjects
Recycled fibers ,Ionic liquids ,Pretreatment ,Brittleness ,Biotechnology ,TP248.13-248.65 - Abstract
In the present study, three ionic liquids, 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), 1-allyl-3-methylimidazolium ([AMIM]Cl), and 1-ethyl-3-methylimidazolium chloride dimethyphosphate ([EMIM]DMP), were used to eliminate the brittleness of recycled fibers. The results showed that the pretreatments with ionic liquids were able to modify and improve the properties of recycled fibers even at high moisture contents. [EMIM]DMP gave better performance compared to [BMIM]Cl and [AMIM]Cl, which can tolerate higher moisture contents. The optimal conditions of EMIM]DMP pretreatment were moisture content of 65%, [EMIM]DMP dosage of 20 wt-%, 80 °C, and 60 min, for which a higher brittleness removal was obtained. The tensile index, bursting index, and tearing index of handsheets were increased by 32.4%, 57.0%, and 46.5%, respectively. Fiber quality was improved as demonstrated by fiber length, lowered fines content, and increased swellability. Such results imply that ionic liquids pretreatment can promote the swelling of recycled fibers and remove their brittleness.
- Published
- 2015
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36. TGF-β1 Promotes Osteosarcoma Cell Migration and Invasion Through the miR-143-Versican Pathway
- Author
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Fengfeng Li, Shaohua Li, and Tao Cheng
- Subjects
Invasion ,Osteosarcoma ,TGF-β1 ,Versican ,MiR-143 ,Migration ,Physiology ,QP1-981 ,Biochemistry ,QD415-436 - Abstract
Background & Aims: TGF-β1 is an abundant cytokine present in the tumour microenvironment. It has been shown to trigger versican expression in human osteosarcoma cells, which may account for the metastatic potential of these cells. However, the underlying mechanism of TGF-β1-mediated metastasis remains unclear. The aim of this study was to evaluate the roles of versican in TGF-β1-induced osteosarcoma cell migration and invasion. Methods: Sixty paired osteosarcoma tumour tissues and adjacent normal tissues were obtained, and the relationship between Enneking stage and versican expression was tested by ANOVA analysis. Real-time PCR or Western blot was used to detect versican, Smad and miR-143 expression. Osteosarcoma cell migration and invasion was assessed using Boyden chambers. A luciferase reporter assay was employed to validate the miR-143-versican interaction. Results: Both versican isoforms, V0 and V1, were significantly differentially expressed in tumours at different stages. TGF-β1 promoted osteosarcoma cell migration and invasion in vitro by up-regulating versican. Furthermore, TGF-β1 suppressed miR-143 expression through a Smad 2/3-dependent pathway. miR-143 directly targets the versican 3'-UTR, and anti-miR-143 or versican knockdown blocked the effects of TGF-β1. Conclusion: Our results suggest that TGF-β1 up-regulates versican expression by suppressing miR-143, and this pathway is important for osteosarcoma cell migration and invasion.
- Published
- 2014
- Full Text
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37. Metabolites contributing to Rhizoctonia solani AG-1-IA maturation and sclerotial differentiation revealed by UPLC-QTOF-MS metabolomics.
- Author
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Wenjin Hu, Xinli Pan, Hafiz Muhammad Khalid Abbas, Fengfeng Li, and Wubei Dong
- Subjects
Medicine ,Science - Abstract
Rhizoctonia solani is a causative agent of sheath blight, which results in huge economic losses every year. During its life cycle, the formation of sclerotia helps Rhizoctonia solani withstand a variety of unfavorable factors. Oxidative stress is a key factor that induces sclerotium formation. The differentiated and undifferentiated phenotypes of R. solani AG-1-IA were obtained by controlling aerial conditions. Metabolomics based on the mass spectrometry technique combined with multivariate and univariate analyses was used to investigate the metabolic variation in vegetative, differentiated and undifferentiated mycelia. Our results revealed that during maturation, the metabolic levels of N2-acetyl-L-ornithine, 3,1'-(OH)2-Gamma-carotene, (5Z,7E)-(1S,3R)-24,24-difluoro-24a-homo-9,10-seco-5,7,10(19)-cholestatrien-1,3,25-triol, stoloniferone O, PA(O-18:0/12:0), PA(P-16:0/14:0), PA(P-16:0/16:(19Z)) and PA(P-16:0/17:2(9Z,12Z)) were suppressed in both differentiated and undifferentiated mycelia. The concentrations of PE(20:1(11Z)/14:1(9Z)), PE(P-16:0/20:4(5Z,8Z,11Z,13E)(15OH[S])) and PS(12:0/18:1(9Z)) were increased in the differentiated group, while increased levels of N(gamma)-nitro-L-arginine, tenuazonic acid and 9S,10S,11R-trihydroxy-12Z,15Z-octadecadienoic acid were found in the undifferentiated group. Our results suggest that different levels of these metabolites may act as biomarkers for the developmental stages of R. solani AG-1-IA. Moreover, the mechanisms of sclerotium formation and mycelium differentiation were elucidated at the metabolic level.
- Published
- 2017
- Full Text
- View/download PDF
38. Compressive Alginate Sponge Derived from Seaweed Biomass Resources for Methylene Blue Removal from Wastewater
- Author
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Xiaojun Shen, Panli Huang, Fengfeng Li, Xiluan Wang, Tongqi Yuan, and Runcang Sun
- Subjects
alginate sponge ,two-step lyophilization ,methylene blue ,adsorption capacity ,biomass resources ,Organic chemistry ,QD241-441 - Abstract
Low cost fabrication of water treatment polymer materials directly from biomass resources is urgently needed in recent days. Herein, a compressive alginate sponge (AS) is prepared from seaweed biomass resources through a green two-step lyophilization method. This material is much different from conventional oven-, air-, vacuum-dried alginate-based adsorbents, which show limitations of shrinkage, rigidness, tight nonporous structure and restricted ions diffusion, hindering its practical applications, and was used to efficiently remove methylene blue (MB), a main colorful contaminant in dye manufacturing, from wastewater. The batch adsorption studies are carried out to determine the impact of pH, contact time and concentration of dye on the adsorption process. The maximum adsorption capacity can be obtained at 1279 mg g−1, and the shape-moldable AS can be facilely utilized as a fixed-bed absorption column, providing an efficient approach for continuous removal of MB within a short time. It is also important that such a compressive AS can be regenerated by a simple squeezing method while retaining about 70% capacity for more than ten cycles, which is convenient to be reused in practical water treatment. Compressive AS demonstrates its merits of high capability, large efficiency and easy to recycle as well as low cost resources, indicating widespread potentials for application in dye contaminant control regarding environmental protection.
- Published
- 2019
- Full Text
- View/download PDF
39. Lentivirus-Mediated ERK2 siRNA Reduces Joint Capsule Fibrosis in a Rat Model of Post-Traumatic Joint Contracture
- Author
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Cunyi Fan, Fengfeng Li, and Shen Liu
- Subjects
post-traumatic joint contracture ,extracellular signal-regulated kinase 2 ,ERK2 ,siRNA ,lentivirus ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Extracellular signal-regulated kinase (ERK)-2 is presumed to play an important role in the development of post-traumatic joint contractures. Using a rat injury model, we investigated whether treatment with ERK2 small interfering RNA (siRNA) could reduce the extent of joint capsule fibrosis after an induced injury. Rats were separated into three groups (n = 32 each): non-operated control group, operated contracture group and contracture-treatment group. Stable post-traumatic joint contracture was created through surgical intra-articular joint injury followed by eight weeks of immobilization. In the contracture-treatment group, the rats were treated with lentivirus (LV)-mediated ERK2 siRNA at days 3 and 7 post-surgery. The posterior joint capsule was assessed by western blotting, immunohistochemistry and biochemical analysis for changes in ERK2, phosphorylated (p)-ERK2, myofibroblast, total collagen and relative collagen Type III expression level. Biomechanical testing was used to assess the development of flexion contractures. Statistical analysis was performed using an analysis of variance. In the operated contracture group, rats that developed flexion contractures also showed elevated phosphorylated p-ERK2 expression. In the contracture-treatment group, ERK2 siRNA significantly reduced p-ERK2 expression levels, as well as the severity of flexion contracture development (p < 0.01). Myofibroblast numbers and measurements of total collagen content were also significantly reduced following ERK2 siRNA (p < 0.01). Relative collagen type III expression as a proportion of total of Types I and III collagen, however, was significantly increased in response to ERK2 siRNA (p < 0.01). Our findings demonstrate a role for ERK2 in the induction of joint capsule fibrosis after injury. Furthermore, we show that development of flexion contractures and the resultant increase of joint capsule fibrosis can be reduced by LV-mediated ERK2 siRNA treatment.
- Published
- 2013
- Full Text
- View/download PDF
40. Prevention of Tendon Adhesions by ERK2 Small Interfering RNAs
- Author
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Cunyi Fan, Xujun Li, Fengfeng Li, Hongjiang Ruan, and Shen Liu
- Subjects
tendon repair ,adhesion ,siRNA ,extracellular signal-regulated kinase (ERK) 2 ,fibroblast proliferation ,lentivirus ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Tendon adhesions are one of the most concerning complications after surgical repair of flexor tendon injury. Extracellular signal-regulated kinase (ERK) 2 plays crucial roles in fibroblast proliferation and collagen expression which contributes to the formation of tendon adhesions after flexor tendon surgery. Using a chicken model, we have examined the effects of a small interfering RNA (siRNA) targeting ERK2 delivered by a lentiviral system on tendon adhesion formation with an adhesion scoring system, histological assessment, and biomechanical evaluation. It was found that ERK2 siRNA effectively suppressed the increase of fibroblasts and the formation of tendon adhesions (p < 0.05 compared with the control group). Moreover, no statistically significant reduction in breaking force was detected between the ERK2 siRNA group and the control group. These results show that the lentiviral-mediated siRNA system is effective in preventing tendon adhesion formation but not to tendon healing, and may be used for tendon repair after confirmation and improvement by future detailed studies.
- Published
- 2013
- Full Text
- View/download PDF
41. Numerical Simulation Study of Dust Transport of Comprehensive Mining Working Surface.
- Author
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Liang Wang, Fengfeng Li, Yuyin Guo, Qingzhen Li, Tianming Chen, and Jingjian Wu
- Published
- 2022
- Full Text
- View/download PDF
42. The high-quality development in Beijing-Tianjin-Hebei regions: Based on the perspective of comparison.
- Author
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Weihong Li, Hongfang Song, Fan Dong, and Fengfeng Li
- Published
- 2021
- Full Text
- View/download PDF
43. Tracing System of Meat Supply Based on RFID and Two-Dimensional Code Technology.
- Author
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Xu Yang, Yongbin Zhao, Ranran Li, Fengfeng Li, and Xiaolin Qi
- Published
- 2018
- Full Text
- View/download PDF
44. Conversion of Bi3+ to Bi2+ in Bi-doped CaSnO3 ceramic phosphors and trap energy-upconversion of Bi2+-doped CaSnO3 for bio-imaging
- Author
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Yue Wang, Weiyan Lei, Shiran Wu, Fengyan Niu, Qisheng He, Yi Shen, and Fengfeng Li
- Subjects
Process Chemistry and Technology ,Materials Chemistry ,Ceramics and Composites ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2023
45. A 4D-printed programmable soft network with fractal design and adjustable hydrophobic performance
- Author
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Nan Li, Wei Zhao, Fengfeng Li, Liwu Liu, Yanju Liu, and Jinsong Leng
- Subjects
General Materials Science - Published
- 2023
46. Synthesis of a CaAl2O4:Eu2+, Dy3+ Phosphor by the Polymer Slurry Method and Its Application in Anticounterfeiting
- Author
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Dongxin Shi, Yue Wang, Nan Yang, Fengfeng Li, Xiaohui Li, Jiahao Ye, Xiaona Zhou, and Baozhong Li
- Subjects
General Chemical Engineering ,General Chemistry ,Industrial and Manufacturing Engineering - Published
- 2023
47. Shape recovery properties and load-carrying capacity of a 4D printed thick-walled kirigami-inspired honeycomb structure
- Author
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Chengbin Yue, Wei Zhao, Fengfeng Li, Liwu Liu, Yanju Liu, and Jinsong Leng
- Subjects
Materials Science (miscellaneous) ,Biomedical Engineering ,Industrial and Manufacturing Engineering ,Biotechnology - Published
- 2023
48. Smart Space Deployable Truss Based on Shape-Memory Releasing Mechanisms and Actuation Laminates
- Author
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Xin Lan, Liwu Liu, Chengtong Pan, Guanghua Hou, Fengfeng Li, Zhengxian Liu, Wenxu Dai, Linlin Wang, Fenghua Zhang, Jian Sun, Honghao Yue, Yanju Liu, Jinsong Leng, Xiaoqing Zhong, and Yong Tang
- Subjects
Space and Planetary Science ,Aerospace Engineering - Abstract
A structure-level smart deployable truss (SDT) was further studied for on-orbit verification on the SJ18 Geostationary Satellite in this work on the basis of a material-level validation of a shape-memory polymer composite (SMPC) sheet on the SJ17 Geostationary Satellite in 2016. In the SDT, a shape-memory alloy (SMA) mechanism was used to replace traditional pyrotechnic devices for locking and releasing, and SMPC actuation laminates were employed to replace traditional motor or spring driving mechanisms. With a strict parameter constrain of mass, size, and stiffness, the current form of the SDT flight hardware was finally obtained through several cycles of optimization design. Its systematical ground-based experiments were conducted, and the overall structural and functional performance met the demands of the aerospace standards of China. The locking force and expansion breaking force of the SMA releasing mechanism were [Formula: see text] and [Formula: see text], ensuring the locking and unlocking reliability for the movable parts with mass 5 kg. The actuation force of all SMPC laminates decreased from [Formula: see text] (0°) to [Formula: see text] (165°) with a recovery duration of [Formula: see text], which was strong enough for SDT deployment in space. The shape-memory materials show great prospects for smart deployable structures in space.
- Published
- 2023
49. Reusable cubic porous light-storing assisted photodegradation composite Sr2MgSi2O7:Eu2+,Dy3+/Ag3PO4 device with adsorption-photocatalysis effects for dark degradation
- Author
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Yue Wang, Shiran Wu, Deheng Li, Weiyan Lei, Yi Shen, and Fengfeng Li
- Subjects
Mechanics of Materials ,Mechanical Engineering ,General Materials Science - Published
- 2022
50. Structural Engineering of Vanadium Oxide Cathodes by Mn2+ Preintercalation for High-Performance Aqueous Zinc-Ion Batteries
- Author
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Fengfeng Li, Hongwei Sheng, Hongyun Ma, Yifeng Qi, Mingjiao Shao, Jiao Yuan, Wenquan Li, and Wei Lan
- Subjects
Materials Chemistry ,Electrochemistry ,Energy Engineering and Power Technology ,Chemical Engineering (miscellaneous) ,Electrical and Electronic Engineering - Published
- 2023
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