103 results on '"Fernández-Cancio, M."'
Search Results
2. Niveles plasmáticos de vitamina D en población autóctona y en poblaciones inmigrantes de diferentes etnias menores de 6 años de edad
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Sánchez Muro, J.M., Yeste Fernández, D., Marín Muñoz, A., Fernández Cancio, M., Audí Parera, L., and Carrascosa Lezcano, A.
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- 2015
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3. Vitamin D and growth hormone regulate growth hormone/insulin-like growth factor (GH–IGF) axis gene expression in human fetal epiphyseal chondrocytes
- Author
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Fernández-Cancio, M., Audi, L., Carrascosa, A., Toran, N., Andaluz, P., Esteban, C., and Granada, M.L.
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- 2009
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4. IGF-I and not IGF-II expression is regulated by glucocorticoids in human fetal epiphyseal chondrocytes
- Author
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Fernandez-Cancio, M., Esteban, C., Carrascosa, A., Toran, N., Andaluz, P., and Audi, L.
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- 2008
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5. Ten Novel Mutations in the NR5A1 Gene Cause Disordered Sex Development in 46,XY and Ovarian Insufficiency in 46,XX Individuals
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Camats, N., Pandey, A. V., Fernández-Cancio, M., Andaluz, P., Janner, M., Torán, N., Moreno, F., Bereket, A., Akcay, T., García-García, E., Muñoz, M. T., Gracia, R., Nistal, M., Castaño, L., Mullis, P. E., Carrascosa, A., Audí, L., and Flück, C. E.
- Published
- 2012
6. SRD5A2 gene mutations and polymorphisms in Spanish 46,XY patients with a disorder of sex differentiation
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Fernández-Cancio, M., Audí, L., Andaluz, P., Torán, N., Piró, C., Albisu, M., Gussinyé, M., Yeste, D., Clemente, M., Martínez-Mora, J., Blanco, A., Granada, M. L., Marco, M., Ferragut, J., López-Siguero, J. P., Beneyto, M., Carles, C., and Carrascosa, A.
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- 2011
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7. Novel (60%) and Recurrent (40%) Androgen Receptor Gene Mutations in a Series of 59 Patients with a 46,XY Disorder of Sex Development
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Audi, L., Fernández-Cancio, M., Carrascosa, A., Andaluz, P., Torán, N., Piró, C., Vilaró, E., Vicens-Calvet, E., Gussinyé, M., Albisu, M. A., Yeste, D., Clemente, M., Hernández de la Calle, I., Del Campo, M., Vendrell, T., Blanco, A., Martínez-Mora, J., Granada, M. L., Salinas, I., Forn, J., Calaf, J., Angerri, O., Martínez-Sopena, M. J., del Valle, J., García, E., Gracia-Bouthelier, R., Lapunzina, P., Mayayo, E., Labarta, J. I., Lledó, G., Sánchez del Pozo, J., Arroyo, J., Pérez-Aytes, A., Beneyto, M., Segura, A., Borrás, V., Gabau, E., Caimarí, M., Rodríguez, A., Martínez-Aedo, M. J., Carrera, M., Castaño, L., Andrade, M., and Bermúdez de la Vega, J. A.
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- 2010
8. The exon 3-deleted/full-length Growth Hormone Receptor Polymorphism Does Not Influence the Effect of Puberty or Growth Hormone Therapy on Glucose Homeostasis in Short Non-Growth Hormone-Deficient Small-for-Gestational-Age Children: Results from a Two-Year Controlled Prospective Study
- Author
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Audí, L, Carrascosa, A, Esteban, C, Fernández-Cancio, M, Andaluz, P, Yeste, D, Espadero, R, Granada, M L., Wollmann, H, and Fryklund, L
- Published
- 2008
9. The Exon 3-Deleted/Full-Length Growth Hormone Receptor Polymorphism Did Not Influence Growth Response to Growth Hormone Therapy over Two Years in Prepubertal Short Children Born at Term with Adequate Weight and Length for Gestational Age
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Carrascosa, A, Audí, L, Fernández-Cancio, M, Esteban, C, Andaluz, P, Vilaró, E, Clemente, M, Yeste, D, Albisu, M A., and Gussinyé, M
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- 2008
10. Molecular Basis of Aromatase Deficiency in a 46, XX Patient with Mutation of Arginine 550 to Tryptophan in POR: Expanding the Endocrine Phenotype in PORD
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Sameer S Udhane, Norio Kagawa, Shaheena Parween, Laura Audí, Sara Benito-Sanz, Velazquez Mnr, Juan-Pedro López-Siguero, Christa E. Flück, Amit V. Pandey, Fernández Cancio M, and Núria Camats
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medicine.medical_specialty ,Arginine ,Tryptophan ,Biology ,medicine.disease ,Phenotype ,3. Good health ,Endocrinology ,Internal medicine ,Mutation (genetic algorithm) ,medicine ,Endocrine system ,Congenital adrenal hyperplasia ,Aromatase deficiency - Abstract
Context: Mutations in Cytochrome P450 oxidoreductase (POR) cause a form of congenital adrenal hyperplasia (CAH). We are reporting a novel R550W mutation in POR identified in a 46, XX patient with signs of aromatase deficiency. Objective: Analysis of aromatase deficiency from R550W mutation in POR. Design, Setting, and Patient: Both the child and the mother had signs of virilization. Ultrasound revealed the presence of uterus and ovaries. No defects in CYP19A1 were found, but further analysis with a targeted Disorders of Sexual Development NGS panel (DSDSeq.V1, 111 genes) on a NextSeq (Illumina) platform in Madrid and Barcelona, Spain, revealed compound heterozygous mutations c.73_74delCT/p.L25FfsTer93 and c.1648C>T/p.R550W in POR. WT and R550W POR were produced as recombinant proteins and tested with multiple cytochrome P450 enzymes at University Children’s Hospital, Bern, Switzerland. Main Outcome Measure and Results: R550W POR showed 41% of the WT activity in cytochrome c and 7.7% activity for reduction of MTT. Assays of CYP19A1 showed a severe loss of activity and CYP17A1, as well as CYP21A2 activities, were also lost by more than 95%. Loss of CYP2C9, CYP2C19, and CYP3A4 activities was observed for the R550W-POR. Predicted adverse effect on aromatase activity as well as a reduction in binding of NADPH was confirmed. Conclusions: Pathological effects due to POR R550W were identified, expanding the knowledge of molecular pathways associated with aromatase deficiency. Screening of the POR gene may provide a diagnosis in CAH without defects in genes for steroid metabolizing enzymes.
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- 2019
11. The d3/fl-Growth Hormone (GH) Receptor Polymorphism Does Not Influence the Effect of GH Treatment (66 μg/kg per Day) or the Spontaneous Growth in Short Non-GH-Deficient Small-for-Gestational-Age Children: Results from a Two-Year Controlled Prospective Study in 170 Spanish Patients
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Carrascosa, A, Esteban, C, Espadero, R, Fernández-Cancio, M, Andaluz, P, Clemente, M, Audí, L, Wollmann, H, Fryklund, L, and Parodi, L
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- 2006
12. VDR gene polymorphism at exon 2 start codon (FokI) may have influenced Type 1 diabetes mellitus susceptibility in two Spanish populations
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Audí, L., Martí, G., Esteban, C., Oyarzabal, M., Chueca, M., Gussinyé, M., Yeste, D., Fernández-Cancio, M., Andaluz, P., and Carrascosa, A.
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- 2004
13. Longitudinal Pubertal Growth According to Age at Pubertal Growth Spurt Onset: Data from a Spanish Study Including 458 Children (223 Boys and 235 Girls)
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Ferrández, A., primary, Carrascosa, A., additional, Audí, L., additional, Baguer, L., additional, Rueda, C., additional, Bosch-Castañé, J., additional, Gussinyé, M., additional, Yeste, D., additional, Labarta, J.I., additional, Mayayo, E., additional, Fernández-Cancio, M., additional, Albisu, M.A., additional, and Clemente, M., additional
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- 2009
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14. Vitamin D Stimulates Growth Hormone-Insulin-Like Growth Factor (GH-IGF) Gene Axis Expression and Potentiates GH Effect to Reverse the Inhibition Produced by Glucocorticoids in Human Growth Plate Chondrocytes
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Fernández-Cancio, M., primary, Andaluz, P., additional, Torán, N., additional, Esteban, C., additional, Carrascosa, A., additional, and Audí, L., additional
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- 2007
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15. Evaluación de la anemia ferropénica en niños menores de 6 años de edad de diferentes etnias.
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Sánchez Muro, J. M., Marín Muñoz, A., Yeste Fernández, D., Fernández Cancio, M., Audí Parera, L., and Carrascosa Lezcano, A.
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IRON deficiency anemia ,HEALTH of immigrants ,NUTRITIONAL anemia ,NUTRITIONAL status ,ANTHROPOMETRY - Abstract
Copyright of Acta Pediátrica Española is the property of Ediciones Mayo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2015
16. Height Gain at Adult-Height Age in 184 Short Patients Treated with Growth Hormone from Prepubertal Age to Near Adult-Height Age is Not Related to GH Secretory Status at GH Therapy Onset.
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Carrascosa, a., audí, L., Fernández-Cancio, M., Yeste, D., Gussinye, M., Campos, a., albisu, M.a., Clemente, M., Bel, J., Nosás, R., Rabanal, M., del Pozo, C., Gómez, J.M., and Mesa, J.
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HUMAN growth ,SHORT stature ,SOMATOTROPIN ,RETROSPECTIVE studies ,CHILD patients ,GROWTH hormone releasing factor ,U-statistics - Abstract
Background: GH release after stimuli classifies short children as severe idiopathic isolated GH deficiency (IIGHD), mild IIGHD, dissociated GH release (DGHR) and normal GH release (NGHR) and anthropometric birth data as adequate for gestational age (AGA) or small for gestational age (SGA). GH release after stimuli classifies AGA patients as IIGHD or as idiopathic short stature (ISS). Aim: To compare height gain induced by GH therapy (31.8 ± 3.5 µg/kg/day, 7.7 ± 1.6 years) started at prepubertal age and stopped at near adult-height age. Methods: A retrospective longitudinal multicenter study including184 short patients classified as severe IIGHD n = 25, mild IIGHD n = 75, DGHR n = 55 and NGHR n = 29; or as IIGHD n = 78, ISS n = 57 and SGA n = 49. Height gain was evaluated throughout GH therapy and at adult-height age. Results: Height-SDS gain at adult-height age was similar among severe IIGHD (1.8 ± 0.8 SDS), mild IIGHD (1.6 ± 0.6 SDS), DGHR (1.7 ± 0.7 SDS) and NGHR (1.6 ± 0.7 SDS), or among IIGHD (1.7 ± 0.7 SDS), ISS (1.7 ± 0.6 SDS) and SGA (1.6 ± 0.8 SD). Conclusion: GH-release stimuli are of little help for deciding on GH therapy in the clinical management of prepubertal children with IIGHD, ISS or SGA. Copyright © 2013 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
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- 2013
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17. Letters VDR gene polymorphism at exon 2 start codon ( FokI) may have influenced Type 1 diabetes mellitus susceptibility in two Spanish populations.
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Audí, L., Marti, G., Esteban, C., Oyarzabal, M., Chueca, M., Gussinyé, M., Yeste, D., Fernández-Cancio, M., Andaluz, P., Carrascosa, A., Goldman, S.M., Viswanathan, V., Sivagami, M., Seena, R., Snehalatha, C., Ramachandran, A., Veves, A., Classen, J.B., and Montgomery, S.M.
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DIABETES ,LETTERS to the editor ,GENETIC polymorphisms ,VITAMIN D ,DIAGNOSTIC errors ,NEUROPATHY ,SPINAL stenosis ,ULCERS - Abstract
Presents several letters to the editor regarding issues on diabetes and diabetic medicine. Discussion on the influence of vitamin D receptor gene polymorphism at exon 2 start codon on type 1 diabetes mellitus susceptibility in Spanish populations; Study on the prevalence of misdiagnosis in diabetic peripheral neuropathy and spinal stenoses; Research on increased forefoot to rearfoot plantar pressure ratio in South Indian patients with diabetic foot ulceration; Consideration of pertussis infections, vaccines in type 1 diabetes.
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- 2004
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18. Clinical, Biochemical and Morphologic Diagnostic Markers in an Infant Male Pseudohermaphrodite Patient with Compound Heterozygous Mutations (G115D/R246W) in SRD5A2 Gene
- Author
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Fernández-Cancio, M., Rodó, J., Andaluz, P., Osaba, M.J. Martínez de, Rodríguez-Hierro, F., Esteban, C., Carrascosa, A., and Audí, L.
- Abstract
Abstract A patient with male pseudohermaphroditism and clinical diagnosis of partial androgen insensitivity in the neonatal period was studied at pubertal age for a molecular diagnosis. Hormone studies were conducted at baseline and under hCG stimulation for testosterone and dihydrotestosterone determinations at 2 months of age. Gonadectomy was performed at 4 months. At the age of 13 years genital skin fibroblasts were studied for androgen binding and 5α-reductase activity and peripheral blood DNA was available for androgen receptor (AR) and 5α-reductase (SRD5A2) gene analysis. Exons 18 of AR gene and exons 15 of SRD5A2 gene were sequenced. AR gene coding sequences were normal. SRD5A2 gene analysis revealed two heterozygote mutations (G115D and R246W), with the mother carrying the G115D and the father the R246W mutations. The compound heterozygote mutations in SRD5A2 gene explained an extremely low 5α-reductase enzyme activity in genital skin fibroblasts. Revision of hormonal data from the neonatal period revealed an increased testosterone-to-dihydrotestosterone ratio at the end of an hCG stimulation test, which concurred with the molecular diagnosis. Testis morphology at 4 months of age was normal. Clinical and biochemical differential diagnosis between partial androgen insensitivity syndrome and 5α-reductase enzyme deficiency is difficult in the neonatal period and before puberty. Our results show that in our patient the testosterone-to-dihydrotestosterone ratio would have adequately orientated the diagnosis. The two mutations in SRD5A2 gene have been described in patients of different lineages, though not in combination to date. Testis morphology showed that, during early infancy, the 5α-reductase deficiency may not have affected interstitial or tubular development.Copyright © 2004 S. Karger AG, Basel- Published
- 2004
19. Surgical feminization. Logical or obligatory?,Feminització quirúrgica. Lògica o obligada?
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Rodó-Salas, J., Audi, L., Fernández-Cancio, M., Sepúlveda, J. A., Xavier Tarrado, Cáceres, F., and Morales, L.
20. The sixth ESPE Growth Plate Working Group Symposium (EUROGROP), June 30th, Rotterdam, the Netherlands, 'A multidisciplinary approach to growth plate biology'
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Emons, J., Phillip, M., Wit, J. M., Lars Sävendahl, Vortkamp, A., Mortier, G., Audí, L., Fernández-Cancio, M., Andaluz, P., Torán, N., Esteban, C., Carrascosa, A., Chagin, A., Wehtje, H., Marchini, A., Rappold, G., Karperien, M., Macrae, V. E., Wong, S. C., Smith, W., Gracie, A., Mcinnes, I., Galea, P., Gardner-Medwin, J., Farquharson, C., Ahmed, S. F., Miclea, R. L., Robanus-Maandag, E. C., Hoogendam, J., Löwik, C. W. G. M., and Perry, R. J.
21. Novel variant in HHAT as a cause of different sex development with partial gonadal dysgenesis associated with microcephaly, eye defects, and distal phalangeal hypoplasia of both thumbs: Case report
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Baz Redón, Noelia, Soler Colomer, Laura, Fernández Cancio, Mónica, Benito-Sanz, Sara, Garrido-Pontnou, Marta, Moline Marimon, Teresa, Clemente Leon, Maria, Camats Tarruella, Nuria, Yeste Fernandez, Diego, Institut Català de la Salut, [Baz-Redón N] Grup de Recerca en Creixement i Desenvolupament, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Pediatria, Ginecologia i Obstetrícia i Medicina Preventiva, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Soler-Colomer L] Unitat d’Endocrinologia Pediàtrica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Fernández-Cancio M, Camats-Tarruella N] Grup de Recerca en Creixement i Desenvolupament, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centre for Biomedical Network Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. [Benito-Sanz S] Centre for Biomedical Network Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. Institute of Medical and Molecular Genetics (INGEMM), Hospital Universitario La Paz, Universidad Autonóma de Madrid, Madrid, Spain. [Garrido M, Moliné T] Servei d’Anatomia Patològica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. [Clemente M, Yeste D] Grup de Recerca en Creixement i Desenvolupament, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament de Pediatria, Ginecologia i Obstetrícia i Medicina Preventiva, Universitat Autònoma de Barcelona, Bellaterra, Spain. Unitat d’Endocrinologia Pediàtrica, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Centre for Biomedical Network Research on Rare Diseases (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain, and Vall d'Hebron Barcelona Hospital Campus
- Subjects
Trastorns del desenvolupament ,Crani - Malformacions ,Turner, Síndrome de ,Endocrinology, Diabetes and Metabolism ,fenómenos fisiológicos::crecimiento y desarrollo::desarrollo sexual [FENÓMENOS Y PROCESOS] ,Endocrine System Diseases::Gonadal Disorders::Disorders of Sex Development::Gonadal Dysgenesis [DISEASES] ,enfermedades del sistema endocrino::trastornos gonadales::trastornos del desarrollo sexual::disgenesia gonadal [ENFERMEDADES] ,Musculoskeletal Diseases::Musculoskeletal Abnormalities::Craniofacial Abnormalities::Microcephaly [DISEASES] ,enfermedades musculoesqueléticas::anormalidades musculoesqueléticas::anomalías craneofaciales::microcefalia [ENFERMEDADES] ,Physiological Phenomena::Growth and Development::Sexual Development [PHENOMENA AND PROCESSES] - Abstract
Different sexual development; Minigene studies Desarrollo sexual diferente; Estudios de minigenes Desenvolupament sexual diferent; Estudis minigènics The palmitoylation of the Hedgehog (Hh) family of morphogens, named sonic hedgehog (SHH), desert hedgehog (DHH), and Indian hedgehog (IHH), is crucial for effective short- and long-range signaling. The hedgehog acyltransferase (HHAT) attaches the palmitate molecule to the Hh; therefore, variants in HHAT cause a broad spectrum of phenotypes. A missense HHAT novel variant c.1001T>A/p.(Met334Lys) was described in a patient first referred for a 46,XY different sexual development with partial gonadal dysgenesis but with microcephaly, eye defects, and distal phalangeal hypoplasia of both thumbs. The in silico analysis of the variant predicted an affectation of the nearest splicing site. Thus, in vitro minigene studies were carried out, which demonstrated that the variant does not affect the splicing. Subsequent protein in silico studies supported the pathogenicity of the variant, and, in conclusion, this was considered the cause of the patient’s phenotype. This study was partly supported by a grant from the Fondo de Investigación Sanitaria (PI15/01647 [to MF-C and SB-S]).
- Published
- 2022
22. Expanding the clinical and genetic spectra of primary immunodeficiency-related disorders with clinical exome sequencing: expected and unexpected findings
- Author
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[Rudilla F] Laboratori d'Immunogenètica i Histocompatibilitat, Banc de Sang i Teixits, Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Franco-Jarava C, Martínez-Gallo M, Aguiló-Cucurull A, Pujol-Borrell R] Recerca en Immunologia Diagnòstica, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’ Immunologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Departament de Biologia Cel•lular, de Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Barcelona, Spain. Jeffrey Model Foundation Excellence Center, Barcelona, Spain. [Garcia-Prat M, Martín-Nalda A, Rivière J, Soler-Palacín P] Jeffrey Model Foundation Excellence Center, Barcelona, Spain. Infecció en el pacient pediàtric Immunodeprimit, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Unitat de Patologia Infecciosa I Immunodeficiències de Pediatria (UPIIP), Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Vidal F] Laboratori d'Immunogenètica i Histocompatibilitat, Banc de Sang i Teixits, Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. CIBER en Investigación Biomédica en Red Enfermedades Cardiovaculares (CIBERCV), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. [Cuscó I, Serra C] Servei de Genètica, Vall d'Hebron Hospital Universitari, Barcelona, Spain. [Baz-Redón N] Grup de Recerca en Creixement i Desenvolupament, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Fernández-Cancio M] Grup de Recerca en Creixement i Desenvolupament, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. CIBER en Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. [Garcia-Patos V] Servei de Dermatologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. [Colobran R] Recerca en Immunologia Diagnòstica, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’ Immunologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Departament de Biologia Cel•lular, de Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Barcelona, Spain. Jeffrey Model Foundation Excellence Center, Barcelona, Spain. Servei de Genètica, Vall d'Hebron Hospital Universitari, Barcelona, Spain and Hospital Universitari Vall d'Hebron
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Immunodeficiència ,Técnicas de Investigación::Técnicas Genéticas::Análisis de Secuencia::Análisis de Secuencia de ADN::Secuenciación Completa del Genoma::Secuenciación del Exoma Completo [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,Investigative Techniques::Genetic Techniques::Sequence Analysis::High-Throughput Nucleotide Sequencing [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA::Whole Genome Sequencing::Whole Exome Sequencing [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Genetic Diseases, Inborn::Primary Immunodeficiency Diseases [DISEASES] ,Otros calificadores::Otros calificadores::/genética [Otros calificadores] ,Técnicas de Investigación::Técnicas Genéticas::Análisis de Secuencia::Secuenciación de Nucleótidos de Alto Rendimiento [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,Other subheadings::Other subheadings::/genetics [Other subheadings] ,enfermedades del sistema inmune [ENFERMEDADES] ,Exons ,Genètica - Tècnica - Published
- 2021
23. Broad phenotypes of disorders/differences of sex development in MAMLD1 patients through oligogenic disease
- Author
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[Flück CE, Sauter KS] Pediatric Endocrinology, Diabetology and Metabolism, Department of Pediatrics and Department of BioMedical Research, Bern University Hospital and University of Bern, Bern, Switzerland. [Audí L, Fernández-Cancio M, Camats N] Grup de Recerca en Creixement i Desenvolupament, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, Madrid, Spain. [Martinez de LaPiscina I] Endocrinology and Diabetes Research Group, BioCruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM, CIBERER, University of the Basque Country (UPV-EHU), Barakaldo, Spain. [Castaño L] Pediatric Endocrinology Section, Cruces University Hospital, Endocrinology and Diabetes Research Group, BioCruces Bizkaia Health Research Institute, CIBERDEM, CIBERER, University of the Basque Country (UPV-EHU), Barakaldo, Spain and Hospital Universitari Vall d'Hebron
- Subjects
Técnicas de Investigación::Técnicas Genéticas [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,Investigative Techniques::Genetic Techniques [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Endocrine System Diseases::Gonadal Disorders::Disorders of Sex Development::Sex Chromosome Disorders of Sex Development [DISEASES] ,Genètica - Tècnica ,Cromosomes sexuals - Anomalies ,Enfermedades del Sistema Endocrino::Trastornos Gonadales::Trastornos del Desarrollo Sexual::Trastornos de los Cromosomas Sexuales del Desarrollo Sexual [ENFERMEDADES] - Published
- 2021
24. Broad phenotypes of disorders/differences of sex development in MAMLD1 patients through oligogenic disease
- Author
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Flück, Christa E., Audí Parera, Laura, Fernández Cancio, Mónica, Sauter, Kay-Sara, Martinez de LaPiscina, Idoia, Castaño, Luis, Camats Tarruella, Nuria, [Flück CE, Sauter KS] Pediatric Endocrinology, Diabetology and Metabolism, Department of Pediatrics and Department of BioMedical Research, Bern University Hospital and University of Bern, Bern, Switzerland. [Audí L, Fernández-Cancio M, Camats N] Grup de Recerca en Creixement i Desenvolupament, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, Madrid, Spain. [Martinez de LaPiscina I] Endocrinology and Diabetes Research Group, BioCruces Bizkaia Health Research Institute, Cruces University Hospital, CIBERDEM, CIBERER, University of the Basque Country (UPV-EHU), Barakaldo, Spain. [Castaño L] Pediatric Endocrinology Section, Cruces University Hospital, Endocrinology and Diabetes Research Group, BioCruces Bizkaia Health Research Institute, CIBERDEM, CIBERER, University of the Basque Country (UPV-EHU), Barakaldo, Spain, and Vall d'Hebron Barcelona Hospital Campus
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Investigative Techniques::Genetic Techniques [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Endocrine System Diseases::Gonadal Disorders::Disorders of Sex Development::Sex Chromosome Disorders of Sex Development [DISEASES] ,técnicas de investigación::técnicas genéticas [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,enfermedades del sistema endocrino::trastornos gonadales::trastornos del desarrollo sexual::trastornos de los cromosomas sexuales del desarrollo sexual [ENFERMEDADES] ,Genètica - Tècnica ,Cromosomes sexuals - Anomalies - Abstract
MAMLD1; Disorders/differences of sex development; Hypospadias MAMLD1; Trastornos/diferencias de desarrollo sexual; Hipospadias MAMLD1; Trastorns/diferències del desenvolupament sexual; Hipospàdies Disorders/differences of sex development (DSD) are the result of a discordance between chromosomal, gonadal, and genital sex. DSD may be due to mutations in any of the genes involved in sex determination and development in general, as well as gonadal and/or genital development specifically. MAMLD1 is one of the recognized DSD genes. However, its role is controversial as some MAMLD1 variants are present in normal individuals, several MAMLD1 mutations have wild-type activity in functional studies, and the Mamld1-knockout male mouse presents with normal genitalia and reproduction. We previously tested nine MAMLD1 variants detected in nine 46,XY DSD patients with broad phenotypes for their functional activity, but none of the mutants, except truncated L210X, had diminished transcriptional activity on known target promoters CYP17A1 and HES3. In addition, protein expression of MAMLD1 variants was similar to wild-type, except for the truncated L210X. We hypothesized that MAMLD1 variants may not be sufficient to explain the phenotype in 46,XY DSD individuals, and that further genetic studies should be performed to search for additional hits explaining the broad phenotypes. We therefore performed whole exome sequencing (WES) in seven of these 46,XY patients with DSD and in one 46,XX patient with ovarian insufficiency, who all carried MAMLD1 variants. WES data were filtered by an algorithm including disease-tailored lists of MAMLD1-related and DSD-related genes. Fifty-five potentially deleterious variants in 41 genes were identified; 16/55 variants were reported in genes in association with hypospadias, 8/55 with cryptorchidism, 5/55 with micropenis, and 13/55 were described in relation with female sex development. Patients carried 1-16 variants in 1-16 genes together with their MAMLD1 variation. Network analysis of the identified genes revealed that 23 genes presented gene/protein interactions with MAMLD1. Thus, our study shows that the broad phenotypes of individual DSD might involve multiple genetic variations contributing towards the complex network of sexual development. This work was supported by grants of the Swiss National Science Foundation (http://www.snf.ch) (320030-146127) to CF, the Instituto de Salud Carlos III (www.isciii.es/; Madrid, Spain) Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER, http://www.ciberer.es/) U-712 to MF-C, the Agency for Management of University and Research Grants (AGAUR; agaur.gencat.cat), Barcelona, Spain (2009SGR31) to LA, and by the Beatriu de Pinós Fellowship 2014 BP-B 00145 (AGAUR, Catalonia, Spain), the Instituto de Salud Carlos III (www.isciii.es/; Madrid, Spain) Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER; http:// www.ciberer.es/) U-712 to NC.
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- 2019
25. Expanding the Clinical and Genetic Spectra of Primary Immunodeficiency-Related Disorders With Clinical Exome Sequencing: Expected and Unexpected Findings
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Francesc Rudilla, Clara Franco-Jarava, Mónica Martínez-Gallo, Marina Garcia-Prat, Andrea Martín-Nalda, Jacques Rivière, Aina Aguiló-Cucurull, Laura Mongay, Francisco Vidal, Xavier Solanich, Iñaki Irastorza, Juan Luis Santos-Pérez, Jesús Tercedor Sánchez, Ivon Cuscó, Clara Serra, Noelia Baz-Redón, Mónica Fernández-Cancio, Carmen Carreras, José Manuel Vagace, Vicenç Garcia-Patos, Ricardo Pujol-Borrell, Pere Soler-Palacín, Roger Colobran, [Rudilla F] Laboratori d'Immunogenètica i Histocompatibilitat, Banc de Sang i Teixits, Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Franco-Jarava C, Martínez-Gallo M, Aguiló-Cucurull A, Pujol-Borrell R] Recerca en Immunologia Diagnòstica, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’ Immunologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Departament de Biologia Cel•lular, de Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Barcelona, Spain. Jeffrey Model Foundation Excellence Center, Barcelona, Spain. [Garcia-Prat M, Martín-Nalda A, Rivière J, Soler-Palacín P] Jeffrey Model Foundation Excellence Center, Barcelona, Spain. Infecció en el pacient pediàtric Immunodeprimit, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Unitat de Patologia Infecciosa I Immunodeficiències de Pediatria (UPIIP), Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Vidal F] Laboratori d'Immunogenètica i Histocompatibilitat, Banc de Sang i Teixits, Barcelona, Spain. Grup de Recerca en Medicina Transfusional, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. CIBER en Investigación Biomédica en Red Enfermedades Cardiovaculares (CIBERCV), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. [Cuscó I, Serra C] Servei de Genètica, Vall d'Hebron Hospital Universitari, Barcelona, Spain. [Baz-Redón N] Grup de Recerca en Creixement i Desenvolupament, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. [Fernández-Cancio M] Grup de Recerca en Creixement i Desenvolupament, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. CIBER en Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), Madrid, Spain. [Garcia-Patos V] Servei de Dermatologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. [Colobran R] Recerca en Immunologia Diagnòstica, Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. Servei d’ Immunologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Departament de Biologia Cel•lular, de Fisiologia i d'Immunologia, Universitat Autònoma de Barcelona, Barcelona, Spain. Jeffrey Model Foundation Excellence Center, Barcelona, Spain. Servei de Genètica, Vall d'Hebron Hospital Universitari, Barcelona, Spain, and Vall d'Hebron Barcelona Hospital Campus
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0301 basic medicine ,Male ,Investigative Techniques::Genetic Techniques::Sequence Analysis::Sequence Analysis, DNA::Whole Genome Sequencing::Whole Exome Sequencing [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Congenital, Hereditary, and Neonatal Diseases and Abnormalities::Genetic Diseases, Inborn::Primary Immunodeficiency Diseases [DISEASES] ,0302 clinical medicine ,Investigative Techniques::Genetic Techniques::Sequence Analysis::High-Throughput Nucleotide Sequencing [ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT] ,Genotype ,Immunology and Allergy ,Medical diagnosis ,Child ,Exome sequencing ,Original Research ,next generation sequencing ,Otros calificadores::Otros calificadores::/genética [Otros calificadores] ,Sequencing panel ,clinical exome sequencing ,Exons ,Middle Aged ,Genètica - Tècnica ,Phenotype ,Child, Preschool ,técnicas de investigación::técnicas genéticas::análisis de secuencias::secuenciación de nucleótidos de alto rendimiento [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,Female ,técnicas de investigación::técnicas genéticas::análisis de secuencias::análisis de secuencias de ADN::secuenciación del genoma completo::secuenciación del exoma completo [TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS] ,primary immunodeficiencies ,Adult ,lcsh:Immunologic diseases. Allergy ,Adolescent ,Primary Immunodeficiency Diseases ,Immunology ,TruSight one ,Computational biology ,DNA sequencing ,03 medical and health sciences ,Young Adult ,Exome Sequencing ,medicine ,Other subheadings::Other subheadings::/genetics [Other subheadings] ,Humans ,Genetic Predisposition to Disease ,Genetic Association Studies ,Immunodeficiència ,Genetic heterogeneity ,business.industry ,genetic variants ,Infant, Newborn ,Genetic Variation ,Infant ,medicine.disease ,mutations ,030104 developmental biology ,Mutation ,Primary immunodeficiency ,enfermedades del sistema inmune [ENFERMEDADES] ,TruSight one sequencing panel ,Differential diagnosis ,business ,lcsh:RC581-607 ,030215 immunology ,Comparative genomic hybridization - Abstract
Inmunodeficiencias primarias; Secuenciación de próxima generación; Secuenciación clínica del exoma Immunodeficiències primàries; Seqüenciació de propera generació; Seqüenciació clínica d’exomes Primary immunodeficiencies; Next generation sequencing; Clinical exome sequencing Primary immunodeficiencies (PIDs) refer to a clinically, immunologically, and genetically heterogeneous group of over 350 disorders affecting development or function of the immune system. The increasing use of next-generation sequencing (NGS) technology has greatly facilitated identification of genetic defects in PID patients in daily clinical practice. Several NGS approaches are available, from the unbiased whole exome sequencing (WES) to specific gene panels. Here, we report on a 3-year experience with clinical exome sequencing (CES) for genetic diagnosis of PIDs. We used the TruSight One sequencing panel, which includes 4,813 disease-associated genes, in 61 unrelated patients (pediatric and adults). The analysis was done in 2 steps: first, we focused on a virtual PID panel and then, we expanded the analysis to the remaining genes. A molecular diagnosis was achieved in 19 (31%) patients: 12 (20%) with mutations in genes included in the virtual PID panel and 7 (11%) with mutations in other genes. These latter cases provided interesting and somewhat unexpected findings that expand the clinical and genetic spectra of PID-related disorders, and are useful to consider in the differential diagnosis. We also discuss 5 patients (8%) with incomplete genotypes or variants of uncertain significance. Finally, we address the limitations of CES exemplified by 7 patients (11%) with negative results on CES who were later diagnosed by other approaches (more specific PID panels, WES, and comparative genomic hybridization array). In summary, the genetic diagnosis rate using CES was 31% (including a description of 12 novel mutations), which rose to 42% after including diagnoses achieved by later use of other techniques. The description of patients with mutations in genes not included in the PID classification illustrates the heterogeneity and complexity of PID-related disorders. This study was funded by Instituto de Salud Carlos III, grants PI14/00405 and PI17/00660, cofinanced by the European Regional Development Fund (ERDF).
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- 2019
26. Genetic and Functional Studies of Patients with Thyroid Dyshormonogenesis and Defects in the TSH Receptor ( TSHR ).
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Yeste D, Baz-Redón N, Antolín M, Garcia-Arumí E, Mogas E, Campos-Martorell A, González-Llorens N, Aguilar-Riera C, Soler-Colomer L, Clemente M, Fernández-Cancio M, and Camats-Tarruella N
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- Adolescent, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Congenital Hypothyroidism genetics, Genetic Association Studies, Genotype, Mutation, Phenotype, Thyrotropin metabolism, Thyrotropin blood, Receptors, Thyrotropin genetics, Receptors, Thyrotropin metabolism, Thyroid Dysgenesis genetics
- Abstract
Genetic defects in the TSH receptor ( TSHR ) can cause poor thyroid differentiation (thyroid dysgenesis) and/or thyroid malfunction (thyroid dyshormonogenesis). The phenotype spectrum is wide: from severe congenital hypothyroidism to mild hyperthyrotropinemia. Over 250 TSHR variants have been published, many uncharacterized in vitro. We aimed to genetically characterize patients with thyroid dyshormonogenesis with TSHR defects and to study in vitro the effect of the genetic variants to establish the genotype-phenotype relationship. Pediatric patients with thyroid dyshormonogenesis (160 patients, Catalan CH neonatal screening program, confirmation TSH range: 18.4-100 mIU/L), were analyzed by a high-throughput gene panel. In vitro studies measuring the TSH-dependent cAMP-response-element activation were performed. Five patients with mild or severe thyroid dyshormonogenesis presented six TSHR variants, two unpublished. Each variant showed a different in vitro functional profile that was totally or partially deleterious. Depending on the genotype, some of the variants showed partial deficiency in both genotypes, whereas others presented a different effect. In conclusion, the percentage of patients with thyroid dyshormonogenesis and candidate variants in TSHR is 3.13%. Our in vitro studies contributed to the confirmation of the pathogenicity of the variants and highlighted the importance of studying the effect of the patient's genotype for a correct diagnostic confirmation.
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- 2024
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27. Patients with Thyroid Dyshormonogenesis and DUOX2 Variants: Molecular and Clinical Description and Genotype-Phenotype Correlation.
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Baz-Redón N, Antolín M, Clemente M, Campos A, Mogas E, Fernández-Cancio M, Zafon E, García-Arumí E, Soler L, González-Llorens N, Aguilar-Riera C, Camats-Tarruella N, and Yeste D
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- Humans, Female, Male, Infant, Newborn, Thyroid Dysgenesis genetics, Thyroid Dysgenesis pathology, Phenotype, Mutation, Genotype, Congenital Hypothyroidism genetics, Neonatal Screening, Thyroxine, Dual Oxidases genetics, Dual Oxidases metabolism, Genetic Association Studies
- Abstract
Thyroid dyshormonogenesis (THD) is a heterogeneous group of genetic diseases caused by the total or partial defect in the synthesis or secretion of thyroid hormones. Genetic variants in DUOX2 can cause partial to total iodination organification defects and clinical heterogeneity, from transient to permanent congenital hypothyroidism. The aim of this study was to undertake a molecular characterization and genotype-phenotype correlation in patients with THD and candidate variants in DUOX2 . A total of 31 (19.38%) patients from the Catalan Neonatal Screening Program presented with variants in DUOX2 that could explain their phenotype. Fifteen (48.39%) patients were compound heterozygous, 10 (32.26%) heterozygous, and 4 (12.90%) homozygous. In addition, 8 (26.67%) of these patients presented variants in other genes. A total of 35 variants were described, 10 (28.57%) of these variants have not been previously reported in literature. The most frequent variant in our cohort was c.2895_2898del/p.(Phe966SerfsTer29), classified as pathogenic according to reported functional studies. The final diagnosis of this cohort was permanent THD in 21 patients and transient THD in 10, according to reevaluation and/or need for treatment with levothyroxine. A clear genotype-phenotype correlation could not be identified; therefore, functional studies are necessary to confirm the pathogenicity of the variants.
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- 2024
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28. Clinical and molecular study of patients with thyroid dyshormogenesis and variants in the thyroglobulin gene.
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Fernández-Cancio M, Antolín M, Clemente M, Campos-Martorell A, Mogas E, Baz-Redón N, Leno-Colorado J, Comas-Armangué G, García-Arumí E, Soler-Colomer L, González-Llorens N, Camats-Tarruella N, and Yeste D
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- Humans, Female, Male, Child, Child, Preschool, High-Throughput Nucleotide Sequencing, Phenotype, Infant, Thyroid Dysgenesis genetics, Mutation, Adolescent, Adult, Infant, Newborn, Thyroglobulin genetics, Congenital Hypothyroidism genetics
- Abstract
Introduction: Defects in any thyroid hormone synthesis steps cause thyroid dyshormonogenesis (THD). THD due to thyroglobulin ( TG ) gene variants is a cause of congenital hypothyroidism (CH) with a wide clinical spectrum, ranging from mild to severe permanent hypothyroidism. We present high-throughput sequencing results of patients with TG variants., Methods: A CH high-throughput sequencing-panel of the main genes involved in the regulation of thyroid hormonogenesis was performed to identify those TG variants that may be related to patient THD phenotype., Results: We identified 21 TG gene variants in 19 patients (11.8%) which could explain their phenotype. Ten of those (47.6%) were not previously described. CH was biochemically severe in these 19 patients. Eight of them were reevaluated after one month of discontinuing LT4 treatment and all had severe permanent hypothyroidism. We also identified another 16 patients who presented heterozygous TG variants, of whom, at reevaluation, five had mild permanent and only one had severe permanent hypothyroidisms., Discussions: In this study, 10 novel and 11 previously reported variants in the TG gene have been identified that could explain the phenotype of 19 patients from non-consanguineous families from a large THD cohort. Although not all these TG gene variants can explain all the patients' THD phenotypes, some of them had severe or mild permanent hypothyroidism at reevaluation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Fernández-Cancio, Antolín, Clemente, Campos-Martorell, Mogas, Baz-Redón, Leno-Colorado, Comas-Armangué, García-Arumí, Soler-Colomer, González-Llorens, Camats-Tarruella and Yeste.)
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- 2024
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29. Primary Ciliary Dyskinesia and Retinitis Pigmentosa: Novel RPGR Variant and Possible Modifier Gene.
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Baz-Redón N, Sánchez-Bellver L, Fernández-Cancio M, Rovira-Amigo S, Burgoyne T, Ranjit R, Aquino V, Toro-Barrios N, Carmona R, Polverino E, Cols M, Moreno-Galdó A, Camats-Tarruella N, and Marfany G
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- Humans, Male, Eye Proteins metabolism, Genes, Modifier, Mutation, Ciliary Motility Disorders genetics, Retinitis Pigmentosa genetics
- Abstract
We report a novel RPGR missense variant co-segregated with a familial X-linked retinitis pigmentosa (XLRP) case. The brothers were hemizygous for this variant, but only the proband presented with primary ciliary dyskinesia (PCD). Thus, we aimed to elucidate the role of the RPGR variant and other modifier genes in the phenotypic variability observed in the family and its impact on motile cilia. The pathogenicity of the variant on the RPGR protein was evaluated by in vitro studies transiently transfecting the mutated RPGR gene, and immunofluorescence analysis on nasal brushing samples. Whole-exome sequencing was conducted to identify potential modifier variants. In vitro studies showed that the mutated RPGR protein could not localise to the cilium and impaired cilium formation. Accordingly, RPGR was abnormally distributed in the siblings' nasal brushing samples. In addition, a missense variant in CEP290 was identified. The concurrent RPGR variant influenced ciliary mislocalisation of the protein. We provide a comprehensive characterisation of motile cilia in this XLRP family, with only the proband presenting PCD symptoms. The variant's pathogenicity was confirmed, although it alone does not explain the respiratory symptoms. Finally, the CEP290 gene may be a potential modifier for respiratory symptoms in patients with RPGR mutations.
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- 2024
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30. Genetics and Natural History of Non-pancreatectomized Patients With Congenital Hyperinsulinism Due to Variants in ABCC8.
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Clemente M, Cobo P, Antolín M, Campos A, Yeste D, Tomasini R, Caimari M, Masas M, García-Arumí E, Fernández-Cancio M, Baz-Redón N, and Camats-Tarruella N
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- Child, Child, Preschool, Humans, Blood Glucose, Blood Glucose Self-Monitoring, Hyperinsulinism genetics, Mutation, Sulfonylurea Receptors genetics, Pancreatectomy adverse effects, Congenital Hyperinsulinism complications, Congenital Hyperinsulinism genetics, Congenital Hyperinsulinism surgery, Diabetes Mellitus etiology, Diabetes Mellitus genetics
- Abstract
Context: Patients with congenital hyperinsulinism due to ABCC8 variants generally present severe hypoglycemia and those who do not respond to medical treatment typically undergo pancreatectomy. Few data exist on the natural history of non-pancreatectomized patients., Objective: This work aims to describe the genetic characteristics and natural history in a cohort of non-pancreatectomized patients with congenital hyperinsulinism due to variants in the ABCC8 gene., Methods: Ambispective study of patients with congenital hyperinsulinism with pathogenic or likely pathogenic variants in ABCC8 treated in the last 48 years and who were not pancreatectomized. Continuous glucose monitoring (CGM) has been periodically performed in all patients since 2003. An oral glucose tolerance test was performed if hyperglycemia was detected in the CGM., Results: Eighteen non-pancreatectomized patients with ABCC8 variants were included. Seven (38.9%) patients were heterozygous, 8 (44.4%) compound heterozygous, 2 (11.1%) homozygous, and 1 patient carried 2 variants with incomplete familial segregation studies. Seventeen patients were followed up and 12 (70.6%) of them evolved to spontaneous resolution (median age 6.0 ± 4 years; range, 1-14). Five of these 12 patients (41.7%) subsequently progressed to diabetes with insufficient insulin secretion. Evolution to diabetes was more frequent in patients with biallelic variants in the ABCC8 gene., Conclusion: The high remission rate observed in our cohort makes conservative medical treatment a reliable strategy for the management of patients with congenital hyperinsulinism due to ABCC8 variants. In addition, a periodic follow-up of glucose metabolism after remission is recommended, as a significant proportion of patients evolved to impaired glucose tolerance or diabetes (biphasic phenotype)., (© The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2023
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31. Five-alpha-reductase type 2 deficiency in Spain.
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Fernández-Cancio M, Audí L, and Yeste D
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- Humans, Spain epidemiology, Oxidoreductases, Diabetes Mellitus, Type 2
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- 2023
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32. Incidence and Prevalence of Children's Diffuse Lung Disease in Spain.
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Torrent-Vernetta A, Gaboli M, Castillo-Corullón S, Mondéjar-López P, Sanz Santiago V, Costa-Colomer J, Osona B, Torres-Borrego J, de la Serna-Blázquez O, Bellón Alonso S, Caro Aguilera P, Gimeno-Díaz de Atauri Á, Valenzuela Soria A, Ayats R, Martin de Vicente C, Velasco González V, Moure González JD, Canino Calderín EM, Pastor-Vivero MD, Villar Álvarez MÁ, Rovira-Amigo S, Iglesias Serrano I, Díez Izquierdo A, de Mir Messa I, Gartner S, Navarro A, Baz-Redón N, Carmona R, Camats-Tarruella N, Fernández-Cancio M, Rapp C, Dopazo J, Griese M, and Moreno-Galdó A
- Abstract
Background: Children's diffuse lung disease, also known as children's Interstitial Lung Diseases (chILD), are a heterogeneous group of rare diseases with relevant morbidity and mortality, which diagnosis and classification are very complex. Epidemiological data are scarce. The aim of this study was to analyse incidence and prevalence of chILD in Spain., Methods: Multicentre observational prospective study in patients from 0 to 18 years of age with chILD to analyse its incidence and prevalence in Spain, based on data reported in 2018 and 2019., Results: A total of 381 cases with chILD were notified from 51 paediatric pulmonology units all over Spain, covering the 91.7% of the paediatric population. The average incidence of chILD was 8.18 (CI 95% 6.28-10.48) new cases/million of children per year. The average prevalence of chILD was 46.53 (CI 95% 41.81-51.62) cases/million of children. The age group with the highest prevalence were children under 1 year of age. Different types of disorders were seen in children 2-18 years of age compared with children 0-2 years of age. Most frequent cases were: primary pulmonary interstitial glycogenosis in neonates (17/65), neuroendocrine cell hyperplasia of infancy in infants from 1 to 12 months (44/144), idiopathic pulmonary haemosiderosis in children from 1 to 5 years old (13/74), hypersensitivity pneumonitis in children from 5 to 10 years old (9/51), and scleroderma in older than 10 years old (8/47)., Conclusions: We found a higher incidence and prevalence of chILD than previously described probably due to greater understanding and increased clinician awareness of these rare diseases., (Copyright © 2021 SEPAR. Publicado por Elsevier España, S.L.U. All rights reserved.)
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- 2022
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33. Seventy eight children born small for gestational age without catch-up growth treated with growth hormone from the prepubertal stage until adult height age. An evaluation of puberty and changes in the metabolic profile.
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Campos-Martorell A, Fernández-Cancio M, Clemente León M, Mogas Viñals E, Fàbregas Martori A, Carrascosa Lezcano A, and Yeste Fernández D
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- Adolescent, Adult, Body Height, Child, Child, Preschool, Female, Humans, Infant, Newborn, Male, Human Growth Hormone therapeutic use, Infant, Small for Gestational Age, Metabolome, Puberty
- Abstract
A wide variation in height gain rate is observed in children small for gestational age (SGA) treated with growth hormone (GH). The aim of this study was to evaluate prepubertal and pubertal growth, height gain attained at adult age and to assess potential predictive factors in catch-up growth. Changes in metabolic profile were also analyzed., Patients and Methods: Seventy-eight children born SGA were treated with a GH median dose of 33.0±2.8mcg/kg/day at a mean age of 7.3±2.0 (boys) and 6.0±1.8 (girls)., Results: Mean height (SDS) at GH onset was -3.31±0.7 for boys and -3.48±0.7 for girls. According to age at pubertal growth spurt onset patients were classified in their pubertal maturity group. Adult height attained expressed in SDS was -1.75±0.7 for boys and -1.69±1.0 for girls, both below the range of their mid-parental height. The greatest height gain occurred during the prepubertal period. Patients with greater height gain were lighter (p<0.001), shorter (p=0.005), and younger (p=0.02) at the start of GH, and also showed a greater increase in growth velocity during the first year on GH (p<0.001). SGA children started puberty at the same age and with the same distribution into pubertal maturity group as the reference population. No relevant GH-related adverse events were reported, including in the insulin resistance parameters evaluated. Differences were found in fasting plasma glucose values, but were without clinical relevance. IGF-I plasma values remained within the safety range., Conclusions: GH therapy is safe and beneficial for SGA children. The response to GH therapy is widely heterogeneous, suggesting that GH should be started at a young age and the GH dose prescribed should be individualized. SGA children started puberty at the same age as the reference population. The only factor that predicts greater adult height is growth velocity during the first year of therapy., (Copyright © 2021 SEEN and SED. Published by Elsevier España, S.L.U. All rights reserved.)
- Published
- 2021
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34. Implementation of a Gene Panel for Genetic Diagnosis of Primary Ciliary Dyskinesia.
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Baz-Redón N, Rovira-Amigo S, Paramonov I, Castillo-Corullón S, Cols Roig M, Antolín M, García Arumí E, Torrent-Vernetta A, de Mir Messa I, Gartner S, Iglesias Serrano I, Caballero-Rabasco MA, Asensio de la Cruz Ó, Vizmanos-Lamotte G, Martín de Vicente C, Martínez-Colls MDM, Reula A, Escribano A, Dasí F, Armengot-Carceller M, Polverino E, Amengual Pieras E, Amaro-Rodríguez R, Garrido-Pontnou M, Tizzano E, Camats-Tarruella N, Fernández-Cancio M, and Moreno-Galdó A
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- Cross-Sectional Studies, Homozygote, Humans, Mutation, Kartagener Syndrome diagnosis
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Introduction: Primary ciliary dyskinesia (PCD) is characterized by an alteration in the ciliary structure causing difficulty in the clearance of respiratory secretions. Diagnosis is complex and based on a combination of techniques. The objective of this study was to design a gene panel including all known causative genes, and to corroborate their diagnostic utility in a cohort of Spanish patients., Methods: This was a multicenter cross-sectional study of patients with a high suspicion of PCD, according to European Respiratory Society criteria, designed around a gene panel for massive sequencing using SeqCap EZ capture technology that included 44 genes associated with PCD., Results: We included 79 patients, 53 of whom had a diagnosis of confirmed or highly probable PCD. The sensitivity of the gene panel was 81.1%, with a specificity of 100%. Candidate variants were found in some of the genes of the panel in 43 patients with PCD, 51.2% (22/43) of whom were homozygotes and 48.8% (21/43) compound heterozygotes. The most common causative genes were DNAH5 and CCDC39. We found 52 different variants, 36 of which were not previously described in the literature., Conclusions: The design and implementation of a tailored gene panel produces a high yield in the genetic diagnosis of PCD. This panel provides a better understanding of the causative factors involved in these patients and lays down the groundwork for future therapeutic approaches., (Copyright © 2020 SEPAR. Publicado por Elsevier España, S.L.U. All rights reserved.)
- Published
- 2021
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35. Phenotypic Variability of Patients With PAX8 Variants Presenting With Congenital Hypothyroidism and Eutopic Thyroid.
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Camats N, Baz-Redón N, Fernández-Cancio M, Clemente M, Campos-Martorell A, Jaimes N, Antolín M, Garcia-Arumí E, Blasco-Pérez L, Paramonov I, Mogas E, Soler-Colomer L, and Yeste D
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- Adolescent, Biological Variation, Population, Child, Congenital Hypothyroidism diagnosis, Congenital Hypothyroidism drug therapy, Congenital Hypothyroidism physiopathology, Female, Follow-Up Studies, Hormone Replacement Therapy, Humans, Infant, Infant, Newborn, Male, Mutation, Neonatal Screening, Phenotype, Thyroid Function Tests, Thyroid Gland diagnostic imaging, Thyroid Gland pathology, Thyroxine therapeutic use, Congenital Hypothyroidism genetics, PAX8 Transcription Factor genetics, Thyroid Gland physiology
- Abstract
Purpose: Thyroid dyshormonogenesis is a heterogeneous group of hereditary diseases produced by a total/partial blockage of the biochemical processes of thyroid-hormone synthesis and secretion. Paired box 8 (PAX8) is essential for thyroid morphogenesis and thyroid hormone synthesis. We aimed to identify PAX8 variants in patients with thyroid dyshormonogenesis and to analyze them with in vitro functional studies., Patients and Methods: Nine pediatric patients with a eutopic thyroid gland were analyzed by the Catalan screening program for congenital hypothyroidism. Scintigraphies showed absent, low, or normal uptake. Only one patient had a hypoplastic gland. On reevaluation, perchlorate discharge test was negative or compatible with partial iodine-organization deficit. After evaluation, 8 patients showed permanent mild or severe hypothyroidism. Massive-sequencing techniques were used to detect variants in congenital hypothyroidism-related genes. In vitro functional studies were based on transactivating activity of mutant PAX8 on a TG-gene promoter and analyzed by a dual-luciferase assays., Results: We identified 7 heterozygous PAX8 exonic variants and 1 homozygous PAX8 splicing variant in 9 patients with variable phenotypes of thyroid dyshormonogenesis. Five were novel and 5 variants showed a statistically significant impaired transcriptional activity of TG promoter: 51% to 78% vs the wild type., Conclusions: Nine patients presented with PAX8 candidate variants. All presented with a eutopic thyroid gland and 7 had deleterious variants. The phenotype of affected patients varies considerably, even within the same family; but, all except the homozygous patient presented with a normal eutopic thyroid gland and thyroid dyshormonogenesis. PAX8 functional studies have shown that 6 PAX8 variants are deleterious. Our studies have proven effective in evaluating these variants., (© The Author(s) 2020. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2021
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36. Immunofluorescence Analysis as a Diagnostic Tool in a Spanish Cohort of Patients with Suspected Primary Ciliary Dyskinesia.
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Baz-Redón N, Rovira-Amigo S, Fernández-Cancio M, Castillo-Corullón S, Cols M, Caballero-Rabasco MA, Asensio Ó, Martín de Vicente C, Martínez-Colls MDM, Torrent-Vernetta A, de Mir-Messa I, Gartner S, Iglesias-Serrano I, Díez-Izquierdo A, Polverino E, Amengual-Pieras E, Amaro-Rodríguez R, Vendrell M, Mumany M, Pascual-Sánchez MT, Pérez-Dueñas B, Reula A, Escribano A, Dasí F, Armengot-Carceller M, Garrido-Pontnou M, Camats-Tarruella N, and Moreno-Galdó A
- Abstract
Primary ciliary dyskinesia (PCD) is an autosomal recessive rare disease caused by an alteration of ciliary structure. Immunofluorescence, consisting in the detection of the presence and distribution of cilia proteins in human respiratory cells by fluorescence, has been recently proposed as a technique to improve understanding of disease-causing genes and diagnosis rate in PCD. The objective of this study is to determine the accuracy of a panel of four fluorescently labeled antibodies (DNAH5, DNALI1, GAS8 and RSPH4A or RSPH9) as a PCD diagnostic tool in the absence of transmission electron microscopy analysis. The panel was tested in nasal brushing samples of 74 patients with clinical suspicion of PCD. Sixty-eight (91.9%) patients were evaluable for all tested antibodies. Thirty-three cases (44.6%) presented an absence or mislocation of protein in the ciliary axoneme (15 absent and 3 proximal distribution of DNAH5 in the ciliary axoneme, 3 absent DNAH5 and DNALI1, 7 absent DNALI1 and cytoplasmatic localization of GAS8, 1 absent GAS8, 3 absent RSPH9 and 1 absent RSPH4A). Fifteen patients had confirmed or highly likely PCD but normal immunofluorescence results (68.8% sensitivity and 100% specificity). In conclusion, immunofluorescence analysis is a quick, available, low-cost and reliable diagnostic test for PCD, although it cannot be used as a standalone test.
- Published
- 2020
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37. Unusual context of CENPJ variants and primary microcephaly: compound heterozygosity and nonconsanguinity in an Argentinian patient.
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Cueto-González AM, Fernández-Cancio M, Fernández-Alvarez P, García-Arumí E, and Tizzano EF
- Abstract
Primary microcephaly (MCPH) is a genetically heterogeneous disorder showing an autosomal recessive mode of inheritance. Patients with MCPH present head circumference values two or three standard deviations (SDs) significantly below the mean for age- and sex-matched populations. MCPH is associated with a nonprogressive mild to severe intellectual disability, with normal brain structure in most patients, or with a small brain and gyri without visceral malformations. We present the case of an adult patient born from Argentinian nonconsanguineous healthy parents. He had a head circumference >5 SD below the mean, cerebral neuroimaging showing hypoplasia of the corpus callosum, bilateral migration disorder with heterotopia of the sylvian fissure and colpocephaly. The patient was compound heterozygous for pathogenic variants in the CENPJ gene (c.289dupA inherited from his mother and c.1132 C > T inherited from his father). Our patient represents an uncommon situation for the usual known context of CENPJ and MCPH, including family origin (Argentinian), pedigree (nonconsanguineous), and genotype (a compound heterozygous case with two variants predicting a truncated protein). Next-generation sequencing studies applied in a broader spectrum of clinical presentations of MCPH syndromes may discover additional similar patients and families., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Author(s) 2020.)
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- 2020
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38. Molecular Basis of CYP19A1 Deficiency in a 46,XX Patient With R550W Mutation in POR: Expanding the PORD Phenotype.
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Parween S, Fernández-Cancio M, Benito-Sanz S, Camats N, Rojas Velazquez MN, López-Siguero JP, Udhane SS, Kagawa N, Flück CE, Audí L, and Pandey AV
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- 46, XX Disorders of Sex Development genetics, Adrenal Hyperplasia, Congenital genetics, Child, Female, Humans, Male, Pedigree, Phenotype, Prognosis, 46, XX Disorders of Sex Development pathology, Adrenal Hyperplasia, Congenital pathology, Aromatase deficiency, Aromatase genetics, Mutation
- Abstract
Context: Mutations in cytochrome P450 oxidoreductase (POR) cause a form of congenital adrenal hyperplasia (CAH). We report a novel R550W mutation in POR identified in a 46,XX patient with signs of aromatase deficiency., Objective: Analysis of aromatase deficiency from the R550W mutation in POR., Design, Setting, and Patient: Both the child and the mother had signs of virilization. Ultrasound revealed the presence of uterus and ovaries. No defects in CYP19A1 were found, but further analysis with a targeted Disorders of Sexual Development NGS panel (DSDSeq.V1, 111 genes) on a NextSeq (Illumina) platform in Madrid and Barcelona, Spain, revealed compound heterozygous mutations c.73_74delCT/p.L25FfsTer93 and c.1648C > T/p.R550W in POR. Wild-type and R550W POR were produced as recombinant proteins and tested with multiple cytochrome P450 enzymes at University Children's Hospital, Bern, Switzerland., Main Outcome Measure and Results: POR-R550W showed 41% of the WT activity in cytochrome c and 7.7% activity for reduction of MTT. Assays of CYP19A1 showed a severe loss of activity, and CYP17A1 as well as CYP21A2 activities were also lost by more than 95%. Loss of CYP2C9, CYP2C19, and CYP3A4 activities was observed for the R550W-POR. Predicted adverse effect on aromatase activity as well as a reduction in binding of NADPH was confirmed., Conclusions: Pathological effects due to POR-R550W were identified, expanding the knowledge of molecular pathways associated with aromatase deficiency. Screening of the POR gene may provide a diagnosis in CAH without defects in genes for steroid metabolizing enzymes., (© Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
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- 2020
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39. Expanding the Clinical and Genetic Spectra of Primary Immunodeficiency-Related Disorders With Clinical Exome Sequencing: Expected and Unexpected Findings.
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Rudilla F, Franco-Jarava C, Martínez-Gallo M, Garcia-Prat M, Martín-Nalda A, Rivière J, Aguiló-Cucurull A, Mongay L, Vidal F, Solanich X, Irastorza I, Santos-Pérez JL, Tercedor Sánchez J, Cuscó I, Serra C, Baz-Redón N, Fernández-Cancio M, Carreras C, Vagace JM, Garcia-Patos V, Pujol-Borrell R, Soler-Palacín P, and Colobran R
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- Adolescent, Adult, Child, Child, Preschool, Female, Genetic Variation, Genotype, Humans, Infant, Infant, Newborn, Male, Middle Aged, Mutation, Phenotype, Primary Immunodeficiency Diseases diagnosis, Exome Sequencing, Young Adult, Genetic Association Studies methods, Genetic Predisposition to Disease, Primary Immunodeficiency Diseases genetics, Primary Immunodeficiency Diseases immunology
- Abstract
Primary immunodeficiencies (PIDs) refer to a clinically, immunologically, and genetically heterogeneous group of over 350 disorders affecting development or function of the immune system. The increasing use of next-generation sequencing (NGS) technology has greatly facilitated identification of genetic defects in PID patients in daily clinical practice. Several NGS approaches are available, from the unbiased whole exome sequencing (WES) to specific gene panels. Here, we report on a 3-year experience with clinical exome sequencing (CES) for genetic diagnosis of PIDs. We used the TruSight One sequencing panel, which includes 4,813 disease-associated genes, in 61 unrelated patients (pediatric and adults). The analysis was done in 2 steps: first, we focused on a virtual PID panel and then, we expanded the analysis to the remaining genes. A molecular diagnosis was achieved in 19 (31%) patients: 12 (20%) with mutations in genes included in the virtual PID panel and 7 (11%) with mutations in other genes. These latter cases provided interesting and somewhat unexpected findings that expand the clinical and genetic spectra of PID-related disorders, and are useful to consider in the differential diagnosis. We also discuss 5 patients (8%) with incomplete genotypes or variants of uncertain significance. Finally, we address the limitations of CES exemplified by 7 patients (11%) with negative results on CES who were later diagnosed by other approaches (more specific PID panels, WES, and comparative genomic hybridization array). In summary, the genetic diagnosis rate using CES was 31% (including a description of 12 novel mutations), which rose to 42% after including diagnoses achieved by later use of other techniques. The description of patients with mutations in genes not included in the PID classification illustrates the heterogeneity and complexity of PID-related disorders., (Copyright © 2019 Rudilla, Franco-Jarava, Martínez-Gallo, Garcia-Prat, Martín-Nalda, Rivière, Aguiló-Cucurull, Mongay, Vidal, Solanich, Irastorza, Santos-Pérez, Tercedor Sánchez, Cuscó, Serra, Baz-Redón, Fernández-Cancio, Carreras, Vagace, Garcia-Patos, Pujol-Borrell, Soler-Palacín and Colobran.)
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- 2019
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40. Broad Phenotypes of Disorders/Differences of Sex Development in MAMLD1 Patients Through Oligogenic Disease.
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Flück CE, Audí L, Fernández-Cancio M, Sauter KS, Martinez de LaPiscina I, Castaño L, Esteva I, and Camats N
- Abstract
Disorders/differences of sex development (DSD) are the result of a discordance between chromosomal, gonadal, and genital sex. DSD may be due to mutations in any of the genes involved in sex determination and development in general, as well as gonadal and/or genital development specifically. MAMLD1 is one of the recognized DSD genes. However, its role is controversial as some MAMLD1 variants are present in normal individuals, several MAMLD1 mutations have wild-type activity in functional studies, and the Mamld1 -knockout male mouse presents with normal genitalia and reproduction. We previously tested nine MAMLD1 variants detected in nine 46,XY DSD patients with broad phenotypes for their functional activity, but none of the mutants, except truncated L210X, had diminished transcriptional activity on known target promoters CYP17A1 and HES3 . In addition, protein expression of MAMLD1 variants was similar to wild-type, except for the truncated L210X. We hypothesized that MAMLD1 variants may not be sufficient to explain the phenotype in 46,XY DSD individuals, and that further genetic studies should be performed to search for additional hits explaining the broad phenotypes. We therefore performed whole exome sequencing (WES) in seven of these 46,XY patients with DSD and in one 46,XX patient with ovarian insufficiency, who all carried MAMLD1 variants. WES data were filtered by an algorithm including disease-tailored lists of MAMLD1 -related and DSD-related genes. Fifty-five potentially deleterious variants in 41 genes were identified; 16/55 variants were reported in genes in association with hypospadias, 8/55 with cryptorchidism, 5/55 with micropenis, and 13/55 were described in relation with female sex development. Patients carried 1-16 variants in 1-16 genes together with their MAMLD1 variation. Network analysis of the identified genes revealed that 23 genes presented gene/protein interactions with MAMLD1. Thus, our study shows that the broad phenotypes of individual DSD might involve multiple genetic variations contributing towards the complex network of sexual development.
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- 2019
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41. Role of Immunofluorescence and Molecular Diagnosis in the Characterization of Primary Ciliary Dyskinesia.
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Baz-Redón N, Rovira-Amigo S, Camats-Tarruella N, Fernández-Cancio M, Garrido-Pontnou M, Antolín M, Reula A, Armengot-Carceller M, Carrascosa A, and Moreno-Galdó A
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- Adolescent, Female, Humans, Ciliary Motility Disorders diagnosis, Fluorescent Antibody Technique, Molecular Diagnostic Techniques methods
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- 2019
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42. Isolated pulmonary interstitial glycogenosis associated with alveolar growth abnormalities: A long-term follow-up study.
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Sardón O, Torrent-Vernetta A, Rovira-Amigo S, Dishop MK, Ferreres JC, Navarro A, Corcuera P, Korta-Murua J, Peña PG, Pérez-Belmonte E, Villares A, Camats N, Fernández-Cancio M, Carrascosa A, Pérez-Yarza EG, and Moreno-Galdó A
- Subjects
- Biopsy, Child, Child, Preschool, Cytoplasm metabolism, Disease Progression, Dyspnea, Female, Follow-Up Studies, Glycogen metabolism, Glycogen Storage Disease complications, Humans, Hypoxia, Infant, Infant, Newborn, Lung diagnostic imaging, Lung Diseases, Interstitial complications, Male, Tachypnea, Tomography, X-Ray Computed, Treatment Outcome, Glycogen Storage Disease diagnosis, Lung Diseases, Interstitial diagnosis, Pulmonary Alveoli pathology
- Abstract
Introduction: Pulmonary interstitial glycogenosis (PIG) is a rare infant interstitial lung disease characterized by an increase in the number of interstitial mesenchymal cells, presenting as enhanced cytoplasmic glycogen, and is considered to represent the expression of an underlying lung development disorder., Methods: This study describes the clinical, radiological, and functional characteristics and long-term outcomes (median 12 years) of nine infants diagnosed with isolated PIG associated with alveolar simplification in the absence of other diseases., Results: All patients presented with tachypnea. Additionally, seven patients had breathing difficulties and hypoxemia. Abnormalities in chest-computerized tomography (CT) with a pattern of ground-glass opacity, septal thickening, and air trapping were observed in all individuals, with images suggesting abnormal alveolar growth (parenchymal bands and architectural distortion). All lung biopsies showed alveolar simplification associated with an increased number of interstitial cells, which appeared as accumulated cytoplasmic glycogen. In the follow-up, all patients were asymptomatic. The respiratory function test was normal in only two patients. Five children showed an obstructive pattern, and two children showed a restrictive pattern. Chest-CT, performed after an average of 6.5 years since the initial investigation, revealed a partial improvement of the ground-glass opacity pattern; however, relevant alterations persisted., Conclusion: Although the patients with PIG in the absence of other associated pathologies had a good clinical outcome, significant radiographic alterations and sequelae in lung function were still observed after a median follow-up of 12 years, suggesting that PIG is a marker of some other persistent abnormalities in lung growth, which have effects beyond the symptomatic period., (© 2019 Wiley Periodicals, Inc.)
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- 2019
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43. A Novel Homozygous AMRH2 Gene Mutation in a Patient with Persistent Müllerian Duct Syndrome.
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Fernández-Cancio M, Viswanath N, Puzhankara R, Valiyaprambil Pavithran P, Mora-Palma C, Camats N, Audí L, and Benito-Sanz S
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- Disorder of Sex Development, 46,XY diagnostic imaging, Homozygote, Humans, Infant, Infant, Newborn, Laparoscopy, Male, Disorder of Sex Development, 46,XY genetics, Mutation genetics, Receptors, Peptide genetics, Receptors, Transforming Growth Factor beta genetics
- Abstract
Persistent müllerian duct syndrome (PMDS) is characterized by the presence of müllerian duct derivatives in otherwise phenotypically normal males. Homozygous or compound heterozygous alterations in AMH or AMHR2 have been identified in approximately 88% of PMDS cases. We report on a male patient with bilateral undescended gonads, müllerian derivatives, and normal serum AMH levels. A novel homozygous missense mutation, c.119G>C;p.Gly40Ala, in exon 2 of AMHR2 was detected that supported the clinical diagnosis of PMDS., (© 2019 S. Karger AG, Basel.)
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- 2019
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44. [Pubertal growth of 1,453 healthy children according to age at pubertal growth spurt onset. The Barcelona longitudinal growth study].
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Carrascosa A, Yeste D, Moreno-Galdó A, Gussinyé M, Ferrández Á, Clemente M, and Fernández-Cancio M
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- Adolescent, Age Factors, Child, Female, Humans, Longitudinal Studies, Male, Reference Values, Spain, Growth, Puberty
- Abstract
Introduction: Pubertal growth pattern differs according to age at pubertal growth spurt onset which occurs over a five years period (girls: 8-13 years, boys: 10-15 years). The need for more than one pubertal reference pattern has been proposed. We aimed to obtain five 1-year-age-interval pubertal patterns., Subjects and Methods: Longitudinal (6 years of age-adult height) growth study of 1,453 healthy children to evaluate height-for-age, growth velocity-for-age and weight-for-age values. According to age at pubertal growth spurt onset girls were considered: very-early matures (8-9 years, n=119), early matures (9-10 years, n=157), intermediate matures (10-11 years, n=238), late matures (11-12 years, n=127) and very-late matures (12-13 years, n=102), and boys: very-early matures (10-11 years, n=110), early matures (11-12 years, n=139), intermediate matures (12-13 years, n=225), late matures (13-14 years, n=133) and very-late matures (14-15 years, n=103). Age at menarche and growth up to adult height were recorded., Results: In both sexes, statistically-significant (P<.0001) and clinically-pertinent differences in pubertal growth pattern (mean height-for-age, mean growth velocity-for-age and mean pubertal height gain, values) were found among the five pubertal maturity groups and between each group and the whole population, despite similar adult height values. The same occurred for age at menarche and growth from menarche to adult height (P<.05)., Conclusions: In both sexes, pubertal growth spurt onset is a critical milestone determining pubertal growth and sexual development. The contribution of our data to better clinical evaluation of growth according to the pubertal maturity tempo of each child will obviate the mistakes made when only one pubertal growth reference is used., (Copyright © 2017 Asociación Española de Pediatría. Publicado por Elsevier España, S.L.U. All rights reserved.)
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- 2018
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45. [Body mass index and tri-ponderal mass index of 1,453 healthy non-obese, non-undernourished millennial children. The Barcelona longitudinal growth study].
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Carrascosa A, Yeste D, Moreno-Galdó A, Gussinyé M, Ferrández Á, Clemente M, and Fernández-Cancio M
- Subjects
- Adolescent, Child, Child, Preschool, Female, Humans, Infant, Longitudinal Studies, Male, Reference Values, Spain, Body Height, Body Mass Index, Body Weight, Growth
- Abstract
Introduction: Body mass index-for age (BMI) and tri-ponderal mass index-for-age (TMI) values of healthy non-underweight, non-obese millennial children have not been reported until now. We aimed to obtain these values., Subjects and Methods: Longitudinal growth study (1995-2017) of 1,453 healthy non-underweight, non-obese millennial children, from birth (n = 477) or from 4 years of age (n = 976) to 18 years in girls and 19 years in boys (25,851 anthropometric measurements)., Results: In each sex, mean BMI-for-age values increased from birth to one year, declined until 5and increased from then onwards. Mean TMI-for-age values decreased abruptly during the first 6years of age and slowly thereafter, in both sexes. Although, at some ages, mean BMI-for age values differed statistically between sexes, differences were scant and of poor clinical significance. The same occurred for TMI-for-age values. BMI-for-age cut-off values to define underweight status (-2 SD) were similar to those proposed by Cole and the WHO for both sexes. However, BMI-for-age cut-off values to define obesity (+2 SD) were lower in both sexes (1.0-5.3) than those proposed by Cole and similar to those proposed by the WHO until 12 in girls and 14 in boys and lower (1.0-4.8) from these ages onwards., Conclusions: BMI-for-age and TMI-for-age values of healthy non-underweight, non-obese millennial children are provided. No clinically relevant differences were observed between sexes. These values may be used to measure underweight status and obesity in present pediatric populations and to evaluate the relationship between BMI-for-age and TMI-for-age in a clinical setting., (Copyright © 2017 Asociación Española de Pediatría. Publicado por Elsevier España, S.L.U. All rights reserved.)
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- 2018
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46. Broad phenotypes in heterozygous NR5A1 46,XY patients with a disorder of sex development: an oligogenic origin?
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Camats N, Fernández-Cancio M, Audí L, Schaller A, and Flück CE
- Subjects
- Disorder of Sex Development, 46,XY pathology, Female, Heterozygote, Humans, Male, Mutation, Disorder of Sex Development, 46,XY genetics, Multifactorial Inheritance, Phenotype, Steroidogenic Factor 1 genetics
- Abstract
SF-1/NR5A1 is a transcriptional regulator of adrenal and gonadal development. NR5A1 disease-causing variants cause disorders of sex development (DSD) and adrenal failure, but most affected individuals show a broad DSD/reproductive phenotype only. Most NR5A1 variants show in vitro pathogenic effects, but not when tested in heterozygote state together with wild-type NR5A1 as usually seen in patients. Thus, the genotype-phenotype correlation for NR5A1 variants remains an unsolved question. We analyzed heterozygous 46,XY SF-1/NR5A1 patients by whole exome sequencing and used an algorithm for data analysis based on selected project-specific DSD- and SF-1-related genes. The variants detected were evaluated for their significance in literature, databases and checked in silico using webtools. We identified 19 potentially deleterious variants (one to seven per patient) in 18 genes in four 46,XY DSD subjects carrying heterozygous NR5A1 disease-causing variants. We constructed a scheme of all these hits within the landscape of currently known genes involved in male sex determination and differentiation. Our results suggest that the broad phenotype in these heterozygous NR5A1 46,XY DSD subjects may well be explained by an oligogenic mode of inheritance, in which multiple hits, individually non-deleterious, may contribute to a DSD phenotype unique to each heterozygous SF-1/NR5A1 individual.
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- 2018
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47. Mechanism of the Dual Activities of Human CYP17A1 and Binding to Anti-Prostate Cancer Drug Abiraterone Revealed by a Novel V366M Mutation Causing 17,20 Lyase Deficiency.
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Fernández-Cancio M, Camats N, Flück CE, Zalewski A, Dick B, Frey BM, Monné R, Torán N, Audí L, and Pandey AV
- Abstract
The CYP17A1 gene regulates sex steroid biosynthesis in humans through 17α-hydroxylase/17,20 lyase activities and is a target of anti-prostate cancer drug abiraterone. In a 46, XY patient with female external genitalia, together with a loss of function mutation S441P, we identified a novel missense mutation V366M at the catalytic center of CYP17A1 which preferentially impaired 17,20 lyase activity. Kinetic experiments with bacterially expressed proteins revealed that V366M mutant enzyme can bind and metabolize pregnenolone to 17OH-pregnenolone, but 17OH-pregnenolone binding and conversion to dehydroepiandrosterone (DHEA) was impaired, explaining the patient’s steroid profile. Abiraterone could not bind and inhibit the 17α-hydroxylase activity of the CYP17A1-V366M mutant. Molecular dynamics (MD) simulations showed that V366M creates a “one-way valve” and suggests a mechanism for dual activities of human CYP17A1 where, after the conversion of pregnenolone to 17OH-pregnenolone, the product exits the active site and re-enters for conversion to dehydroepiandrosterone. The V366M mutant also explained the effectiveness of the anti-prostate cancer drug abiraterone as a potent inhibitor of CYP17A1 by binding tightly at the active site in the WT enzyme. The V366M is the first human mutation to be described at the active site of CYP17A1 that causes isolated 17,20 lyase deficiency. Knowledge about the specificity of CYP17A1 activities is of importance for the development of treatments for polycystic ovary syndrome and inhibitors for prostate cancer therapy.
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- 2018
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48. GATA4 Variants in Individuals With a 46,XY Disorder of Sex Development (DSD) May or May Not Be Associated With Cardiac Defects Depending on Second Hits in Other DSD Genes.
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Martinez de LaPiscina I, de Mingo C, Riedl S, Rodriguez A, Pandey AV, Fernández-Cancio M, Camats N, Sinclair A, Castaño L, Audi L, and Flück CE
- Abstract
Disorders of sex development (DSD) consist of a wide range of conditions involving numerous genes. Nevertheless, about half of 46,XY individuals remain genetically unsolved. GATA4 gene variants, mainly related to congenital heart defects (CHD), have also been recently associated with 46,XY DSD. In this study, we characterized three individuals presenting with 46,XY DSD with or without CHD and GATA4 variants in order to understand the phenotypical variability. We studied one patient presenting CHD and 46,XY gonadal dysgenesis, and two patients with a history of genetically unsolved 46,XY DSD, also known as male primary hypogonadism. Mutation analysis was carried out by candidate gene approach or targeted gene panel sequencing. Functional activity of GATA4 variants was tested in vitro on the CYP17 promoter involved in sex development using JEG3 cells. We found two novel and one previously described GATA4 variants located in the N-terminal zinc finger domain of the protein. Cys238Arg variant lost transcriptional activity on the CYP17 promoter reporter, while Trp228Cys and Pro226Leu behaved similar to wild type. These results were in line with bioinformatics simulation studies. Additional DSD variations, in the LRP4 and LHCGR genes, respectively, were identified in the two 46,XY individuals without CHD. Overall, our study shows that human GATA4 mutations identified in patients with 46,XY DSD may or may not be associated with CHD. Possible explanations for phenotypical variability may comprise incomplete penetrance, variable sensitivity of partner genes, and oligogenic mechanisms.
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- 2018
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49. Development of Laboratory Investigations in Disorders of Sex Development.
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Audí L, Camats N, Fernández-Cancio M, and Granada ML
- Subjects
- Animals, Cytogenetic Analysis, Disease Models, Animal, Disorders of Sex Development genetics, Genetic Association Studies, Hormones analysis, Hormones isolation & purification, Humans, Clinical Laboratory Techniques methods, Disorders of Sex Development diagnosis
- Abstract
Scientific knowledge to understand the biological basis of sex development was prompted by the observation of variants different from the 2 most frequent body types, and this became one of the fields first studied by modern pediatric endocrinology. The clinical observation was supported by professionals working in different areas of laboratory sciences which led to the description of adrenal and gonadal steroidogenesis, the enzymes involved, and the different deficiencies. Steroid hormone measurements evolved from colorimetry to radioimmunoassay (RIA) and automated immunoassays, although gas and liquid chromatography coupled to mass spectrometry are now the gold standard techniques for steroid measurements. Peptide hormones and growth factors were purified, and their measurement evolved from RIA to automated immunoassays. Hormone action mechanisms were described, and their specific receptors were characterized and assayed in experimental materials and in patient tissues and cell cultures. The discovery of the genetic basis for variant sex developments began with the description of the sex chromosomes. Molecular technology allowed cloning of genes coding for the different proteins involved in sex determination and development. Experimental animal models aided in verifying the roles of proteins and also suggested new genes to be investigated. New candidate genes continue to be described based on experimental models and on next-generation sequencing of patient DNAs., (© 2017 S. Karger AG, Basel.)
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- 2018
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50. Nutritional rickets: vitamin D, calcium, and the genetic make-up.
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El Kholy M, Elsedfy H, Fernández-Cancio M, Hamza RT, Amr NH, Ahmed AY, Toaima NN, and Audí L
- Subjects
- 25-Hydroxyvitamin D3 1-alpha-Hydroxylase genetics, Alleles, Case-Control Studies, Child Nutrition Sciences, Child, Preschool, Cholestanetriol 26-Monooxygenase genetics, Cytochrome P450 Family 2 genetics, Female, Genotype, Homozygote, Humans, Infant, Male, Polymorphism, Single Nucleotide, Prospective Studies, Receptors, Calcitriol genetics, Vitamin D-Binding Protein genetics, Vitamin D3 24-Hydroxylase genetics, Calcium blood, Child Nutrition Disorders genetics, Rickets diagnosis, Rickets genetics, Vitamin D blood, Vitamin D Deficiency genetics
- Abstract
Background: The prevalence of vitamin D (vitD) deficiency presenting as rickets is increasing worldwide. Insufficient sun exposure, vitD administration, and/or calcium intake are the main causes. However, vitD system-related genes may also have a role., Methods: Prospective study: 109 rachitic children completed a 6-mo study period or until rachitic manifestations disappeared. Thirty children were selected as controls. Clinical and biochemical data were evaluated at baseline in patients and controls and biochemistry re-evaluated at radiological healing. Therapy was stratified in three different protocols. Fifty-four single-nucleotide polymorphisms (SNPs) of five vitD system genes (VDR, CP2R1, CYP27B1, CYP24A1, and GC) were genotyped and their association with clinical and biochemcial data was analyzed., Results: Therapy response was similar in terms of radiological healing although it was not so in terms of biochemical normalization. Only VDR gene (promoter, start-codon, and intronic genotypes) was rickets-associated in terms of serum 25-OH-D, calcium, radiological severity and time needed to heal. Eight patients with sufficient calcium intake and 25-OH-D levels carried a VDR genotype lacking minor allele homozygous genotypes at SNPs spread along the gene., Conclusion: Although patients presented epidemiologic factors strongly contributing to rickets, genetic modulation affecting predisposition, severity, and clinical course is exerted, at least in part, by VDR gene polymorphic variation.
- Published
- 2017
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