30 results on '"Ferreira CO"'
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2. Tracing the origins and signatures of selection of antifolate resistance in island populations of Plasmodium falciparum
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Pinto João, Cravo Pedro, do Rosário Virgílio E, Galvão André, Teófilo Vânia, Ferreira Conceição, Vicente José L, and Salgueiro Patrícia
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Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Resistance of the malaria parasite Plasmodium falciparum to sulfadoxine-pyrimethamine (SP) has evolved worldwide. In the archipelago of São Tomé and Principe (STP), West Africa, although SP resistance is highly prevalent the drug is still in use in particular circumstances. To address the evolutionary origins of SP resistance in these islands, we genotyped point mutations at P. falciparum dhfr and dhps genes and analysed microsatellites flanking those genes. Methods Blood samples were collected in July and December 2004 in three localities of São Tomé Island and one in Principe Island. Species-specific nested-PCR was used to identify P. falciparum infected samples. Subsequently, SNPs at the dhfr and dhps genes were identified through PCR-RFLP. Isolates were also analysed for three microsatellite loci flanking the dhfr gene, three loci flanking dhps and four loci located at putative neutral genomic regions. Results An increase of resistance-associated mutations at dhfr and dhps was observed, in particular for the dhfr/dhps quintuple mutant, associated with clinical SP failure. Analysis of flanking microsatellites suggests multiple independent introductions for dhfr and dhps mutant haplotypes, possibly from West Africa. A reduced genetic diversity and increased differentiation at flanking microsatellites when compared to neutral loci is consistent with a selective sweep for resistant alleles at both loci. Conclusions This study provides additional evidence for the crucial role of gene flow and drug selective pressures in the rapid spread of SP resistance in P. falciparum populations, from only a few mutation events giving rise to resistance-associated mutants. It also highlights the importance of human migration in the spread of drug resistant malaria parasites, as the distance between the islands and mainland is not consistent with mosquito-mediated parasite dispersal.
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- 2010
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3. Raised houses reduce mosquito bites
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Gil Vilfrido, Ferreira Conceicao, Sousa Carla A, Ferrara Patrica R, Pinto Joao, Charlwood J, and do Rosário Virgillo E
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background In many parts of continental Africa house construction does not appear to impede entry of malaria vectors and, given their generally late biting cycle, the great majority of transmission takes place indoors. In contrast, many houses in São Tomé, 140 km off the coast of Gabon, are raised on stilts and built of wooden planks. Building on stilts is a time-honoured, but largely untested, way of avoiding mosquito bites. Exposure may also be affected by mosquito activity times and age composition of host-seeking females. A study was therefore undertaken on the island of São Tomé to determine if exposure to Anopheles gambiae, the only vector on the island, varied with house construction or time of the night. Methods A series of all-night landing collections were undertaken out of doors at ground level, inside houses at ground level, on the verandas of, and inside houses built on stilts. The gonotrophic age of an unselected sample of insects from the first three hours of landing collection (18:00–21:00) was determined by dissection. In addition, 1,149 miniature light-trap collections were obtained from 125 houses in the study area. Numbers collected were related to house construction. Results Biting of An. gambiae took place primarily outside at ground level. Less than one third of biting occurred inside houses. Houses built on stilts had half the number of An. gambiae in them compared to those built at ground level. Conversely houses with an eaves gap had more An. gambiae in them than houses without such a gap. Gonotrophic age did not affect house entry rates in An. gambiae. House construction affected Culex quinquefasciatus less than An. gambiae. Mean density per house, derived from a series of 1,490 randomly assigned light-trap collections, was over-dispersed with 18% of houses having 70% of the vectors. Conclusion House construction plays an important role in determining exposure to malaria vectors in São Tomé. Neighbours can have very different exposure levels. Recommendations for improvement in control are given.
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- 2003
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4. Malaria and its possible control on the island of Príncipe
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Ferreira Conceição, Gil Vilfrido, Charlwood J Derek, Hagmann Reto, do Rosário Virgíllo, and Smith Tom A
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Malaria can be eradicated from islands. To assess the prospects for eradication of malaria from the island of Príncipe in the Gulf of Guinea, we fitted a mathematical model to age-prevalence curves and thus obtained estimates of the vectorial capacity and of the basic reproductive number (R0) for malaria. Methods A cross-sectional malariological survey was carried out, in mid-1999, in six communities, comprising circa 17% of the total 6,000 population of the island. All houses in these communities were registered and their mode of construction recorded. Thick and thin blood films were prepared from all consenting individuals. Each individual was asked whether they possessed a mosquito net, whether they had slept under a mosquito net the previous night, whether they were allergic to chloroquine, and whether they had visited the main island of São Tomé since the beginning of the year. Outpatient records from March 1999 until the end of December 2000 were also examined and the age and place of residence of diagnosed cases noted. Results 203 (19.8%) of the 1,026 individuals examined were found to be infected with Plasmodium falciparum. By fitting the mathematical model of the Garki project to the age-prevalence curve we estimate that the basic reproductive number, R0, on the island is approximately 1.6. Over a period of one year, a total of 1,792 P. falciparum cases reported to an outpatient facility at the island's hospital. Overall, 54% of the people interviewed slept under mosquito nets and were at reduced risk of infection. Conversely, people living in houses with openings between the top of the wall and the roof had higher risk of infection. Conclusion This high incidence suggests that most of the malaria cases on the island attend the hospital and that treatment of these cases is an important factor reducing the effective rate of transmission. Providing that clinical cases are effectively treated, endemic malaria can probably be eliminated from the island mainly by reducing exposure to the vector with simple measures such as insecticide-treated nets and mosquito-proofing of dwellings. In contrast to traditional malaria eradication strategies, this would avoid the risk of malaria epidemics because the reduction in R0 should be sustainable.
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- 2003
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5. 'A mate or a meal' – Pre-gravid behaviour of female Anopheles gambiae from the islands of São Tomé and Príncipe, West Africa
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Ferreira Conceicao, Sousa Carla A, Pinto Joao, Charlwood J Derek, Petrarca Vincenso, and Rosario Virgilio do E
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Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Malaria prevalence differs between the two islands that comprise the archipelago of São Tomé and Príncipe. This may be due to differences in the biology of local Anopheles gambiae, the only vector on the islands. Survival rate and feeding frequency are two factors influencing vectorial capacity. Anophelines generally feed just once per gonotrophic (oviposition) cycle. Newly emerged insects, however, may feed two or more times during their first oviposition cycle thus increasing the likelihood of becoming infected. The reasons for multiple feeding are not clearly understood and it is still uncertain whether the behaviour is facultative or obligatory. We, therefore, determined survival and sporozoite rates, and examined the behaviour of An. gambiae from the two islands during their first gonotrophic cycle. Methods The wing size of 1,410, abdominal condition of 687, gonotrophic age and mated status of 7,264 female M form An. gambiae collected by light-trap, landing catch, resting outdoors or in copula, was determined from four sites in the archipelago. Sporozoite rates assessed by ELISA in 15,533 females from São Tomé and 2,111 from Príncipe were determined. Results Estimated survival rates ranged between 0.834–0.849 per day in São Tomé and 0.801–0.818 per day in Príncipe. Sporozoite rates of 0.63% in São Tomé were significantly higher than the 0.24% from Príncipe. Overall 49% of females mated on the second night after emergence before feeding, and 51% on the third night and thus fed before mating. The likelihood of mating before feeding increased with wing size. None of the 3,776 parous insects collected showed evidence of recent mating. All but two of the 198 females collected in copula had undeveloped ovaries. Mean wing sizes and the number of insects collected in a sentinel light-trap varied but the proportion of newly emerged insects in the collection did not. The estimated survival rate of the smallest insects was lower than other size groups, but the overall size distribution of each age group was normal. Parous insects were gonotrophically concordant. Conclusion Differences in mosquito survival contributed to the lower sporozoite rates and endemicity of malaria on Príncipe compared to São Tomé. On both islands all newly emerged insects blood fed on the second night following emergence but only became gonotrophically active on the third night after emergence. Smaller insects had a higher 'mortality/emigration' rate than larger ones. We suggest that insufficiency of Juvenile Hormone until the third day of adult life is responsible for gonotrophic inactivity and that by partitioning mating between the second or third day after emergence females maximise their chances of out-crossing.
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- 2003
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6. Habitação flexivel à transição da vida
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Liberato, Maria Neves, Afonso, José Manuel dos Santos, and Mendes, Maria Manuela Ferreira, co-orientadora
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Património industrial ,Habitação flexivel ,Flexible housing ,Industrial heritage ,Manutenção militar - Abstract
Dissertação de Mestrado Integrado em Arquitetura, com a especialização em Arquitetura de Interiores e Reabilitação do Edificado, apresentada na Faculdade de Arquitetura da Universidade de Lisboa para obtenção do grau de Mestre. Submitted by Pilar Lago (mpilar@fa.utl.pt) on 2017-11-15T16:45:28Z No. of bitstreams: 1 Documento Definitivo.pdf: 208810147 bytes, checksum: 56cea0e1f19ee32cecba44e6e5da03bb (MD5) Made available in DSpace on 2017-11-15T16:45:28Z (GMT). No. of bitstreams: 1 Documento Definitivo.pdf: 208810147 bytes, checksum: 56cea0e1f19ee32cecba44e6e5da03bb (MD5) Previous issue date: 2017-07-25 N/A
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- 2017
7. O equipamento colectivo e o espaço público como estratégia de requalificação em AUGI
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Carvalho, Ana Margarida Ataíde Gaspar de, Rodrigues, Pedro Dias Pimenta, and Afonso, Luís Filipe Ferreira, co-orientador
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Urban areas of illegal genesis ,Colective equipament ,Equipamento colectivo ,Espaço público ,Public space ,Centro cívico ,Civic center ,AUGI ,Requalificação urbana - Abstract
Dissertação de Mestrado Integrado em Arquitetura, com a especialização em Arquitetura apresentada na Faculdade de Arquitetura da Universidade de Lisboa para obtenção do grau de Mestre. Em Portugal o crescimento industrial atraiu grandes contingentes populacionais vindos das zonas rurais que se dirigiram para a cidade em busca de melhores condições de vida e de trabalho. Este fenómeno migratório refletiu-se num crescimento demográfico acentuado que provocou uma procura habitacional crescente, obrigando à expansão das cidades para as suas periferias. Dada a incapacidade de resposta por parte do sector legal às crescentes necessidades habitacionais, devido essencialmente à inexistência de uma política eficaz de produção de solo urbano e de habitação, surge como resposta um sector ilegal de produção de habitação que teve a sua maior expressão nos anos 70. Foi neste contexto que surgiram as Áreas Urbanas de Génese Ilegal (AUGI), anteriormente designadas por bairros clandestinos, que se foram expandindo de forma desordenada causando graves consequências ao nível social, urbanístico, económico e institucional. Estas áreas caracterizam-se essencialmente pela sua estrutura urbana descontínua, carente de planeamento e ligação à envolvente. Têm uma enorme dependência da grande cidade por serem áreas dormitório mono funcionais, uma vez que existe um domínio do uso residencial em detrimento de outros usos como o comércio e serviços, espaços públicos e equipamentos colectivos, comprometendo a qualidade de vida social e urbana e contribuindo para um enfraquecimento identitário e isolamento dos indivíduos. A área de estudo e local de intervenção deste trabalho incidiu sobre a AUGI do Bairro do Casal Novo, em Odivelas, sob o tema o equipamento colectivo e o espaço público como estratégia de requalificação. De forma a intervir neste território e apresentar uma proposta de requalificação urbana e arquitectónica capaz de responder às suas carências, desenvolveu-se um discurso teórico e crítico sobre o fenómeno das AUGI e sobre os elementos da estrutura urbana capazes de estruturar, caracterizar e qualificar o território. A proposta urbana passou então por reestruturar e dotar o Bairro do Casal Novo de elementos de carácter público inexistentes, capazes de estruturar o território, criando novas centralidades e referências, tornando assim o bairro parte integrante da cidade. No projecto de arquitectura, desenvolveu-se o Centro Cívico e espaço público associado com o qual se pretendeu qualificar o território criando uma nova centralidade, capaz de gerar identidade e de constituir uma referência orientadora no lugar, dotado de usos e funções necessárias à população. Com esta intervenção pretendeu-se, não só uma integração urbana, mas também uma integração social com o intuito de fixar os residentes, proporcionando uma coexistência social e uma maior qualidade de vida. ABSTRACT: In Portugal industrial growth attracted large population from rural areas who went to the city in search of better conditions of life and work. This migratory phenomenon was reflected in a sharp population growth which caused a growing housing demand, forcing the expansion of cities to their peripheries. Considering the unresponsiveness of the legal sector to the growing housing needs, mainly due to the absence of an effective policy of urban land production and housing, an illegal sector of housing production arises with greater expression in the 1970s. In this context, the Urban Areas of Illegal Genesis (UAIG) emerged, formerly known as clandestine quarters, which were expanding in a disorderly way, causing serious consequences in the social, urban, economic and institutional levels. These areas are characterized mainly by their discontinuous urban structure, lacking in planning and connection to the surrounding ones. They are very dependent on the big city because they are mono functional dormitory areas, since there is a residential predominance over other uses such as trade and services, public spaces and collective facilities, compromising the quality of social and urban life and contributing to a weakening of identities and isolation of individuals. The study area and local intervention of this paper focused on the UAIG of Casal Novo Quarter, in Odivelas, under the theme “the collective equipment and the public space as a requalification strategy”. In order to intervene in this area and submit a proposal for urban and architectural requalification able to answer its deficiencies, a theoretical and critical discourse on the phenomenon of UAIG and on the elements of the urban structure that structure, characterize and qualify the territory was developed. The urban proposal was to restructure and equip the Casal Novo Quarter with nonexistent public elements, able to structure the territory, creating new centers and references, thus making the quarter a part of the city. In the architectural design, the Civic Center and the public space with it associated was developed intending to qualify the territory creating a new center, capable of generating identity and be a guiding reference in the place, provided with uses and functions necessary to the population. With this intervention it was intended not only an urban integration but also a social integration in order to fix the residents, providing a social coexistence and a higher quality of life. N/A
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- 2017
8. Ocorrência de otite externa em cães apresentados à consulta de rotina
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Sampaio, Maria Saraiva de, Faísca, Pedro, orient., Santos, Ana Sofia Pereira dos, orient., and Alves, Maria Margarida Ferreira, co-orient.
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CÃES ,VETERINÁRIA ,OTITIS ,OTITES ,MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA ,CYTOLOGY ,CITOLOGIA ,DOGS ,MEDICINA VETERINÁRIA ,CANIDS ,VETERINARY MEDICINE ,CANÍDEOS ,CLINICAL EXAMINATION ,EXAMES CLÍNICOS - Abstract
A otite externa é uma doença dermatológica de grande importância e frequente em clínica de pequenos animais. O objectivo principal deste estudo foi detectar a presença de otite numa população canina apresentada à consulta vacinal de rotina. Os objectivos secundários foram detectar os factores associados ao desenvolvimento da otite, caracterizar os sinais clínicos e a flora microbiana, e avaliar o conhecimento dos proprietários sobre esta doença. Este estudo foi realizado no período de Outubro de 2013 a Fevereiro de 2014, em duas instituições Médico Veterinárias situadas no distrito de Lisboa, no âmbito do estágio curricular do Mestrado Integrado de Medicina Veterinária. A amostra deste estudo foi constituída por cinquenta cães, dos quais dezassete apresentavam otite externa aguda. A amostra foi avaliada através da história clínica, exame otológico, exame otoscópico e exame citológico. Os proprietários não detectaram a presença de otite externa nos seus animais, apesar de terem observado comportamento de prurido, tendo sido esta a indicação mais comum de otite externa neste estudo. Os casos de otite ocorreram com maior frequência em cães com orelhas pendulares e em cães com hábitos de banho. A presença de lesões observadas na abertura do pavilhão auricular foram pouco relacionadas com os casos de otite, contudo as alterações otoscópicas, estiveram maioritariamente presentes nos casos de otite externa. As otites infecciosas representaram a maioria dos casos, tal como a presença de um grande número de Malassezia spp. e coccus, sempre associados com material ceruminoso., Otitis externa is a dermatologic condition of great importance that appears frequently in small animal practice. The primary goal of this study was to evaluate a canine population, presented to vaccine routine consultation in order to determine an external otitis. The secondary goals were to relate the associated factors with the development of otitis, to consider the clinical signs and microbial flora, and to evaluate the owners’ knowledge. This study was accomplished during the period of October 2013 to February 2014, in two Veterinary institutions situated in the district of Lisbon, within the curricular internship of integrated Masters in Veterinary Medicine. The sample of this study was composed of fifty dogs, of which seventeen exhibited external acute otitis; the sampling was evaluated through a clinical history, an otologic examination, an otoscopic examination and a cytological examination. The owners didn’t obtain knowledge of an external otitis, even when they observed a pruritus behavior, being the pruritus the most common indication of otitis externa in this study. Otitis cases occurred with more frequency in dogs with dropped ears and in dogs with bath’s habits. The presence of lesions observed in the opening of the eardrum were few related with the otitis cases, although the otoscopic alterations, were mainly present in cases of external otitis. The infectious otitis accounted in the majority of cases, such as the presence of a huge number of Malassezia spp. and coccus, always associated with ceruminous material., Orientação : Pedro Faísca ; Co-orient. : Ana Oliveira
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- 2014
9. Diversidade genética e estrutura de população entre isolados de Corynespora cassiicola no Estado do Amazonas
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FERREIRA, A. F. T. A. F. e and ANA FRANCISCA TIBÚRCIA AMORIM FERREIRA E FERREIRA. Co-orientador: Gilvan Ferreira da Silva, CPAA.
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Reprodução Assexuada ,Ascomycota ,Estrutura de população ,Diversidade - Abstract
Made available in DSpace on 2019-01-29T23:46:36Z (GMT). No. of bitstreams: 1 DissertacaoAnaFranciscaTiburciaAmorimFerreiraeFerreira.pdf: 1180916 bytes, checksum: b56caa611e2200f9bb6618c1c14d4f43 (MD5) Previous issue date: 2015-06-24 Dissertação (Mestrado em Agronomia Tropical) - Universidade Federal do Amazonas, Manaus. Orientadora: Jânia Lília da Silva Bentes; Coorientador: Gilvan Ferreira da Silva.
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- 2014
10. Pesquisa de coxiella burnetii em animais de companhia em contexto urbano
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Duarte, Luís Filipe Raposo, Santos, Ana Sofia Pereira dos, orient., and Alves, Maria Margarida Ferreira, co-orient.
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GATOS ,IMUNOFLUORESCÊNCIA ,FELÍDEOS ,CÃES ,FLUORESCENT ANTIBODY TECHNIQUE ,VETERINÁRIA ,BACTÉRIAS ,FELIDS ,MESTRADO INTEGRADO EM MEDICINA VETERINÁRIA ,PCR ,DOGS ,MEDICINA VETERINÁRIA ,CANIDS ,BACTERIA ,CATS ,VETERINARY MEDICINE ,CANÍDEOS - Abstract
A infeção por Coxiella burnetii é considerada uma zoonose emergente ou reemergente, por toda a Europa. Estudos epidemiológicos e clínicos apontam para um aumento de casos de febre Q, em especial em meio rural, tendo como principal fonte de infeção as espécies pecuárias, nomeadamente os ruminantes. No entanto, a possibilidade de existir uma fonte de infeção alternativa é cada vez maior, em especial em infeções adquiridas em meio urbano, nas quais os animais de companhia, cães e gatos, podem ter um papel chave pelo estreito contacto que habitualmente têm com os humanos. Neste trabalho pretendeu-se averiguar se C. burnetii é uma bactéria prevalente, em animais de companhia em contexto urbano. Para tal recolheram-se amostras de 173 cães e 102 gatos, do sexo feminino, para pesquisa de ADN de C. burnetii e anticorpos anti-C. burnetii em fase I e II. Recorreu-se à técnica de PCR nested para pesquisa de ADN em úteros (N=176), sangue (N=167), zaragatoas vaginais (N= 68), placentas (N= 24) e amostras atípicas (N=4), tendo o resultados sido negativo na população em estudo. Os anticorpos anti-C. burnetii foram pesquisados em amostras de plasma (N= 149) com recurso à técnica de IFA utilizando um limiar de positividade de 50, tendo-se obtido resultados positivos em três cães (4,3%) e seis gatos (7,5%) com títulos a variar entre, Coxiella burnetii infection is considered an emerging or re-emerging zoonosis throughout Europe. Epidemiological studies and case reports point to an increase in Q fever cases especially in rural areas, where livestock species are the main source of infection. However, the possibility of an alternative source of infection is increasing, particularly for infections acquired in urban environment, in which companion animals, dogs and cats, may have a key role due to the close contact that most often present with humans. With this work we intended to investigate whether C. burnetii is a common bacteria in companion animals in an urban context. For that samples were collected from 173 dogs and 102 cats females to search for the presence of C. burnetii DNA and anti-C. burnetii phase I and II antibodies. Nested PCR was used for DNA screening in uterus (N= 176), blood (N=167), vaginal swabs (N= 68), placentas (N= 24) and atypical samples (N= 4); all the animals in the studied population presented negative results. Antibodies against C. burnetii were investigated in plasma samples (N= 149) using the IFA technique with a cut-off value of 50, presenting positive results in three dogs (4.3%) and six cats (7.5 %) with titles ranging from, Orientação : Ana Sofia Pereira dos Santos ; Co-orientação : Maria Margarida Ferreira Alves
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- 2014
11. Evaluation of the coronary infiltration in temporary protheses using differents cements
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Lemos, Káthia Gomes, Nunes, Eduardo,Orientador, Silveira, Frank Ferreira,Co-orientador, and Pontifícia Universidade Católica de Minas Gerais.Programa de Pós-Graduação em Odontologia,Instituição
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Cimentos dentários-Teses ,Infiltração dentária-Teses ,616.314.18 ,Hidróxido de cálcio-Teses - Abstract
Dissertação (mestrado) - Pontifícia Universidade Católica de Minas Gerais, Programa de Pós-Graduação em Odontologia Bibliografia: f. 78-81 Resumo: Durante a terapia endodôntica, é de fundamental importância que se promova um selamento eficiente, minimizando a possibilidade de infiltração e contaminação do sistema de canais radiculares. Em dentes portadores de próteses provisórias associadas a retentores intra-radiculares é muito importante a obtenção da qualidade estética e funcional, sendo que a manutenção de um selamento eficiente mostra-se crítica. Este trabalho teve por objetivo avaliar a capacidade seladora de três diferentes cimentos associados a próteses provisórias e retentor intra-radicular, em promover o selamento coronário de dentes com pasta de hidróxido de cálcio como curativo intracanal. Foram selecionados 45 incisivos centrais superiores extraídos de humanos, que, após serem submetidos ao preparo químico-mecânico padronizados, foram preenchidos com curativo de hidróxido de cálcio com soro fisiológico nos 5 mm apicais. Posteriormente ao seccionamento das coroas, os espécimes foram divididos em 3 grupos de 13. Pinos e restaurações provisórias foram fixados com cimentos à base de pasta zinco eugenólica, óxido de zinco e eugenol e à base de hidróxido de cálcio. Como grupo negativo (próteses provisórias fixadas com cimento e totalmente impermeabilizadas) foram selecionados 3 dentes e como grupo controle positivo (próteses provisórias fixadas sem cimento e ausência de impermeabilização) mais 3 dentes. Toda superfície externa das raízes e coroas foi impermeabilizada, exceto 2 mm próximos à adaptação cervical das mesmas. Após serem imersos em Rodamina B a 2% por 7 dias em estufa bacteriológica a 37C, os espécimes foram seccionados longitudinalmente e as infiltrações analisadas com lupa estereomicroscópica Wilde M8 com câmara digital acoplada e análise das imagens utilizando o software Image Pro-Plus. Os resultados tabulados foram submetidos à análise estatística pertinente. Nenhum dos cimentos testados foi capaz de impedir a infiltração do corante; evidenciou-se valores intermediários de infiltração para o Dycal; Lysanda apresentou os menores valores de infiltração. Palavras-chave: Cimentos dentários; Hidróxido de cálcio; Infiltração dentária. Abstract: During endodontic therapy, an efficient sealer is extremely important to minimize the possibility of infiltration and contamination of the root canal system. In teeth bearing temporary protheses associated with intra-radicular retainers, the obtaining of aesthetic and functional quality is very important as the maintenance of an efficient sealer is critical. This article has the aim of evaluating the sealing capacity of three different cements associated with temporary prostheses and intra-radicular retainers in promoting the coronary sealing of teeth using a calcium hydroxide paste as an intracanal dressing. Forty-five central maxillary incisors extracted from human beings were selected. After having undergone standard chemico-mechanical preparation, these incisors were filled with the calcium hydroxide dressing, together with saline solution, in 5mm apicals. After sectioning of the crowns, the specimens were divided in 3 groups of 13. Pins and temporary restorations were fixed with cement consisting of a eugenolic zinc paste (made up of zinc oxide and eugenol) as well as of calcium hydroxide. Three teeth were selected to form the negative control group (temporary prostheses fixed with cement and made completely impermeable), while 3 more teeth were selected to form the positive control group (temporary prostheses fixed without cement and an absence of impermeability). The entire external surface of the roots and crowns, except for a 2mm area near the cervical adaptation, was made impermeable,. After being immersed in a 2% Rodamine B solution for 7 days in a bacteriological greenhouse at 37C, the specimens were longitudinally sectioned and the infiltrations were analyzed using a Wilde M8 stereomicroscopic magnifying glass with an attached digital camera. In addition, the analyses of images were verified using the Pro-plus Image software. All results were submitted to pertinent statistical analyses. None of the cements tested was capable of impeding the infiltration of the dye; it was evidenced intermediate values of infiltration goes Dycal; Lysanda presented the less values of infiltration. Key-words: Calcium hydroxide, Dental cements, Dental infiltration.
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- 2007
12. Análise ambiental por geoprocessamento:estudo de caso da micro Bacia do Ribeirão da Mutuca Nova Lima - MG/Magda Braga de Souza ; orientador: Heinz Charles Kohler ; co-orientador Sérgio dos Anjos Ferreira Pinto
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Souza, Magda Braga de, Kohler, Heinz Charles, Orientador, Pinto, Sérgio dos Anjos Ferreira Co-orientador, and Pontifícia Universidade Católica de Minas Gerais.Programa de Pós-Graduação em Geografia - Tratamento da Informação EspacialInstituição
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681.3 [91] ,Bacias hidrográficas-Teses ,Solo-Uso-Teses ,Sistemas de informação geográfica-Teses - Abstract
Dissertação (Mestrado) - Pontifícia Universidade Católica de Minas Gerais, Programa de Pós-Graduação em Geografia - Tratamento da Informação Espacial Bibliografia: f. 106-114
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- 2007
13. Analysis of scanning electron microscopic the removal of smear layer : use the EDTA alone or interleaved with sodium hypochlorite
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Beraldo, Ângelo José da Silva, Nunes, Eduardo,Orientador, Silveira, Frank Ferreira,Co-Orientador, and Pontifícia Universidade Católica de Minas Gerais.Programa de Pós-Graduação em Odontologia,Instituição
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Ácido etilenodiaminotetraacético-Teses ,Microscopia eletrônica de varredura-Teses ,Endodontia-Teses ,616.314.18 - Abstract
Dissertação (mestrado) - Pontifícia Universidade Católica de Minas Gerais, Programa de Pós-Graduação em Odontologia Inclui bibliografia Resumo: As soluções irrigadoras desempenham um importante papel na terapia endodôntica, auxiliando na limpeza e na anti-sepsia do sistema de canais radiculares. Neste trabalho foram utilizados dentes unirradiculados extraídos de humanos instrumentados utilizando-se limas ProTaper. Em um grupo empregou-se como líquido irrigador NaOCl a 2,5% e EDTA 17% no final, em outro grupo o EDTA foi utilizado, intercalando-se durante a instrumentação com o NaOCl a 2,5%, no grupo controle utilizou-se soro fisiológico. Posteriormente os espécimes foram clivados em duas hemi-partes, as quais foram metalizadas e levadas para análise ao microscópio eletrônico de varredura, quando avaliou-se a presença ou ausência da smear layer nos terços: cervical, médio e apical, dando escores que variavam de 1 a 3. Os dados obtidos foram submetidos ao estudo estatístico não paramétrico Mann-Whitney, sendo observado que nos terços cervical e médio não houve diferença na remoção da smear layer. No terço apical, constatou-se que a utilização do EDTA intercalada por NaOCl na instrumentação, propiciou uma melhor remoção da smear layer. Abstract: The irrigation solution plays an important role in the endodontic therapy, supporting the cleaning and the antiseptic condition of the root canal system. In this study, extracted human single rooted teeth were instrumented with the Protaper files. In one group, NaOCl was used as irrigant solution and 17 % EDTA was used in the end of the instrumentation for a final irrigation. In another group EDTA was used intercaleted with the instrumentation and with 2.5% NaOCl irrigation. Subsequently, the specimens were divided into two hemi parts Those parts were metallized and led to the analysis in the scanning electron microscope. Then, the presence or not of the smear layer in the apical, midle and cervical third was evaluated. All the data were submitted to the Mann-Whitney nonparametric statistical method . It was observed that in the cervical and middle third there were no diference in the smear layer removal. In the apical third, was observed that the use of EDTA intercaleted with the instrumentation and with NaOCl irrigation, propiciated a better removal of the smear layer
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- 2006
14. Desenvolvimento de uma técnica sensorial descritiva por ordenação
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Vanessa Bragato Richter, Marta de Toledo Benassi ., Sandra Helena Prudêncio Ferreira [Co-orientador]., Tereza Cristina Avancini de Almeida, and Rui Sérgio dos Santos Ferreira da Silva
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A análise sensorial descritiva requer treinamento longo e caro dos provadores para fornecer resultados confiáveis e consistentes, contudo, métodos alternativos para obter este tipo de informação são limitados. Os testes de Ordenação, tradicionalmente utilizados como discriminativos, são de fácil aplicação, rapidamente executados e podem ser realizados sobre atributos específicos do produto. Este trabalho teve por objetivo propor um teste sensorial Descritivo por Ordenação e analisar sua eficiência comparando-o com outras técnicas sensoriais e instrumentais tradicionais. Como amostras foram empregados quatro pudins de chocolate com açúcar e dietéticos com diferentes características sensoriais. Para caracterização das amostras foram utilizados testes instrumentais de cor e textura e três diferentes técnicas sensoriais descritivas: Perfil Livre, Análise Descritiva Quantitativa (ADQ) e, o método proposto, Análise Descritiva por Ordenação (ADO). Participaram da avaliação 47 provadores, sendo 14 para o Perfil Livre, 12 para o ADQ e 21 para o ADO. Para o ADQ e ADO, os provadores, selecionados pela capacidade discriminativa, desenvolveram inicialmente uma lista de descritores e as definições correspondentes. Foram depois divididos em duas equipes: uma recebeu treinamento para realizar a ADQ e outra foi familiarizada com os procedimentos da técnica Descritiva por Ordenação. As duas equipes avaliaram os mesmos atributos e os resultados foram avaliados por ANOVA (ADQ) e Teste de Friedman (ADO). Uma terceira equipe caracterizou as amostras empregando o Perfil Livre. Os resultados das três técnicas foram avaliados por Análise Procrustes Generalizada (GPA), que também foi utilizada para verificar o consenso nas equipes. Observou-se que apesar da equipe do ADQ ter sido submetida a treinamento quantitativo, seleção e retreinamento, a configuração das amostras e a variância no comportamento dos provadores foram similares a obtida no Perfil Livre. A equipe do ADO descreveu as amostras de maneira semelhante e, devido a facilidade do procedimento, apresentou maior consenso em comparação com as outras técnicas. Os atributos de aparência (cor marrom) e sabor (chocolate e amargo residual) que apresentaram as maiores correlações no GPA, foram utilizados pelas três equipes na descrição das amostras. Os provadores do ADO e ADQ conseguiram utilizar os descritores de aroma e textura de forma mais consensual que a equipe do Perfil. O ADQ foi a técnica que demonstrou melhor correlação com a análise de textura instrumental. A Análise Descritiva por Ordenação permitiu discriminação das amostras estudadas semelhante às técnicas de ADQ e Perfil Livre e, apesar de utilizar maior número de provadores, apresentou como vantagem o menor custo associado à necessidade de um menor número de sessões que o ADQ e menor quantidade de amostras que as outras técnicas sensoriais tradicionais. The descriptive sensory analysis requires long and expensive training of the assessors to provide reliable and consistent results, however alternative methods to obtain this kind of information are limited. Ranking tests, traditionally used as discriminative, are easier to perform, rapidly executed and can be carried out on specific product attributes. The objective of this work was to propose a ranking descriptive sensorial test and analyze its effectiveness comparing it with other traditional sensory and instrumental techniques. Four chocolate puddings (regular and dietetic ones) with different sensory characteristics were used as samples. For sample characterization instrumental analysis of color and texture and three different descriptive sensory techniques were used: Free-choice Profile, Quantitative Descriptive Analysis (QDA) and, the provided method, Ranking Descriptive Analysis (RDA). 47 subjects participated in the evaluation, being 14 for the Free-choice Profile, 12 for the QDA and 21 for the RDA. In QDA and RDA, the assessors, selected by their discriminative capacity, developed initially a list of attributes and the correspondent definitions. After they were divided into two groups: one received training to perform the QDA and the other was familiarized with the procedures of the Ranking Descriptive Technique. The two groups used the same attributes and the results were evaluated by ANOVA (QDA) and Friedman test (RDA). The third group characterized the samples using Free-choice Profile. The results of the three techniques were evaluated by Generalized Procrustes Analysis (GPA), which was also used to verify the agreement in the panels. It was observed that in spite of the QDA panel had been submitted to quantitative training, selection and re-training, the samples characterization and the variance in the behavior of the assessors were similar to the obtained in the Free-choice Profile. The RDA panel described the samples in a similar way and due to the facility in the procedure showed a greater consensus between assessors compared to the other techniques. Attributes of appearance (brown color) and taste (chocolate and residual bitter), which showed the greatest correlation in the GPA, were used by the three panels in the description of the samples. The assessors of the RDA and QDA got to use the attributes of taste and texture with more agreement than the Free-choice Profile panel. The QDA was the technique that showed the best correlation with the instrumental analysis. The Ranking Descriptive Analysis allowed the discrimination of the samples studied similar to the techniques of the QDA and the Free-choice Profile. In spite of using the greatest number of assessors, it showed as an advantage the lower cost associated to the necessity of a lower number of sessions than the QDA and less amount of samples than the other traditional sensory techniques.
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- 2006
15. Staining capacity of coronal microleakage of three dyes
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Heleno, Juliana Francisca Grossi, Nunes, Eduardo,Orientador, Silveira, Frank Ferreira,Co-Orientador, and Pontifícia Universidade Católica de Minas Gerais.Programa de Pós-Graduação em Odontologia,Instituição
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Canal radicular-Tratamento-Teses ,Corantes-Teses ,Infiltração dentária-Teses ,Endodontia-Teses ,616.314.18 - Abstract
Dissertação (mestrado) - Pontifícia Universidade Católica de Minas Gerais, Programa de Pós-Graduação em Odontologia Bibliografia: f. 129-141 Resumo: Constata-se na literatura uma carência de estudos entre as diferentes soluções marcadoras para avaliar sua eficácia na verificação da microinfiltração marginal coronária. O presente trabalho teve como objetivo realizar uma análise comparativa da capacidade marcadora dos corantes Azul de Metileno 2%, Rodamina B 2% e Sulfato de Níquel 5%, utilizando o cimento Coltosol como material selador temporário. Oitenta e quatro dentes pré-molares unirradiculados extraídos de humanos foram divididos em 6 grupos de 14 espécimes cada, sendo 12 experimentais, um para controle positivo e outro para controle negativo. Após o preparo biomecânico dos canais, os dentes foram impermeabilizados com duas camadas de resina epóxi e uma de esmalte para unhas, exceto em 1mm ao redor do selamento provisório. As amostras foram imersas nas soluções corantes e mantidas em estufa a 37°C por três e sete dias, e submetidas à ciclagem térmica. Neste período, trezentos ciclos (5ºC, 55°C) de 30 segundos cada foram realizados em aparelho específico com programador digital de temperatura, tempo e número de ciclos. Secções longitudinais foram obtidas no sentido vestíbulo-lingual e a penetração dos corantes foi medida através de uma lupa estereomicroscópica (Wild M8). Os resultados estatísticos pela Análise de Variância (ANOVA) mostraram diferenças significativas (p
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- 2004
16. A comunicação visual e gráfica como uma chave de leitura da identidade nacional portuguesa no século XIX
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Ladeiro, Dulce Helena Amorim de Campos, Silva, Fernando José Carneiro Moreira da, and Roda, Rui Miguel Ferreira, co-orientador
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Cultura visual ,Imprensa ilustrada portuguesa do séc. XIX ,Simbólica da identidade nacional portuguesa ,Comunicação visual - Abstract
Tese de Doutoramento em Design, apresentada na Faculdade de Arquitetura da Universidade de Lisboa para obtenção do grau de Doutor. O século XIX é considerado por muitos teóricos um período de expansão de ideologias nacionalistas por toda a Europa que acabariam por originar os atuais estados-nação. Esta conjuntura decorre essencialmente da Revolução Francesa e da Revolução Industrial, as quais produziriam alterações políticas, económicas e sociais tais, que viriam a modificar radicalmente as estruturas dos antigos países, alterando a relação que os indivíduos tinham para com a sua pátria: em vez de uma sociedade organizada à volta de Estados dinásticos, a sociedade passou a ser organizada à volta de um Estado, cujos representantes políticos passaram a ser eleitos pelos cidadãos. Em Portugal este fenómeno desenvolve-se após a revolução liberal de 1820. Em 1822 foi instituída uma Monarquia Constitucional e finalmente em 1834 o liberalismo triunfou. A nova conceção do mundo carecia de novos modos de representação, novas formas expressivas de comunicação e os intelectuais oitocentistas consideravam-se os mais bem preparados para realizar esta transformação. Utilizaram para isso todos os meios artísticos e todas as suas possibilidades criativas para construir uma nova narrativa da nação em toda a sua especificidade concreta que depois ensinaram e difundiram no sentido de alcançar uma consciência nacional mais alargada. Este projeto de investigação tem como tópico investigativo perceber como é que a comunicação visual da imprensa ilustrada se relacionou com as narrativas do nacionalismo cultural e da identidade nacional portuguesa durante o século XIX em Portugal. Mais concretamente pretendeu-se explorar e descrever a função que a comunicação visual dos periódicos ilustrados teve, enquanto ferramenta projetual na instrução, difusão e fixação, de elementos gráficos e símbolos de identidade nacional portuguesa durante o período assinalado. Neste sentido a investigação definiu-se a partir de uma hipótese e configurou-se num estudo exploratório e descritivo com a utilização de uma metodologia não intervencionista de base dominantemente qualitativa com o recurso ao método de estudo de caso múltiplo. O objeto de estudo foi circunscrito a uma amostra de três casos, que são três periódicos ilustrados portugueses dentro do arco temporal predeterminado: O Panorama (1837-1868), o Archivo Pittoresco (1857-1868), e O Occidente (1877-1915). Os três periódicos ilustrados, foram analisados individualmente em função de quatro proposições elaboradas especificamente para direcionar a atenção para aquilo que se pretendia examinar em relação à problemática da tese. Acreditamos que esta investigação vai ajudar a compreender a comunicação visual da imprensa ilustrada do século XIX, enquanto ferramenta projetual, que ao longo do século foi destacando, organizando e difundindo as narrativas imagéticas e os símbolos mais representativos da nação portuguesa. Esperamos com este estudo contribuir para criar uma maior consciência do valor e importância da cultura simbólica da Identidade Nacional Portuguesa no âmbito do projeto perante um mundo cada vez mais globalizado. ABSTRACT : The 19th century is considered by many theoretics as a period of expansion of nationalist ideologies across Europe, which eventually gave rise to the present nation-states. These circumstances were essentially a result of the French Revolution and the Industrial Revolution, which led to political, economic and social changes that were to radically alter the structures of the old countries, changing the relationship between the individuals and their homelands: instead of being organized around dynastic States, society came to be organized around a State whose political representatives were elected by the citizens. In Portugal this phenomenon emerged after the liberal revolution in 1820. In 1822 a Constitutional Monarchy was institued and finally in 1834 the liberalism triumphed. This new conception of the world demanded new methods of representation, new expressive forms of communication and the 19th-century intellectuals considered themselves best prepared to accomplish this transformation. In order to do that, they used all the available artistic means and creative possibilities to build a new narrative of the nation in all its objective specificity, which was subsequently taught and disseminated in order to achieve a broader national awareness. This research project is focused on understanding the relationship between the visual communication of the illustrated press and the narratives of cultural nationalism and national identity in 19th-century Portugal. More specifically, we aimed at exploring and describing the role played by visual communication in illustrated periodicals as a design-related tool for the instruction, dissemination and consolidation of graphic elements and symbols of the Portuguese national identity during the aforementioned period. In this regard, the research work was defined based on a hypothesis and developed as an exploratory and descriptive study conducted using a non-interventionist methodology with a predominantly qualitative perspective approached with the multiple case study method. The object of study was limited to a sample of three cases corresponding to three illustrated periodicals published during the predefined timeframe: O Panorama (1837-1868), o Archivo Pittoresco (1857-1868) and O Occidente (1877-1915). These three illustrated periodicals were analysed individually according to four propositions drawn up specifically to direct our attention to what we intended to examine within the scope of the topic of the thesis. We believe that this research work will contribute for the understanding of the visual communication of the 19th-century illustrated press as a design tool that, throughout the century, highlighted, organized and disseminated the imagetic narratives and the most representative symbols of the Portuguese nation. With this study, we hope to contribute to create a greater awareness of the value and importance of the symbolic culture of the Portuguese National Identity within the scope of design in an increasingly globalized world. FCT - Fundação para a Ciência e a Tecnologia N/A
17. Simultaneous Takotsubo syndrome and spontaneous coronary artery dissection: a case report.
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Ferreira CO, Oliveira CC, Braga CG, and Marques J
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A 59-year-old woman presented to the emergency room with sudden onset of retrosternal thoracic pain following emotional stress. The electrocardiogram (ECG) revealed T-wave inversions on precordial leads. Her blood analyses demonstrated elevation of myocardial necrosis markers (peak of troponin I of 3.4 ng/ml). Transthoracic echocardiogram (TTE) findings were consistent with Takotsubo syndrome, accompanied by mild left ventricular dysfunction. The patient underwent invasive coronary angiography revealing a spontaneous coronary artery dissection in the left anterior descending artery and left main artery. A repeat TTE one week later showed complete resolution of the segmental contractility with a full recovery of left ventricular function. Cardiac magnetic resonance imaging revealed no abnormalities. The patient was discharged on dual-antiplatelet therapy. A follow-up coronary angiography performed one month later confirmed complete resolution of the dissection. Takotsubo syndrome and spontaneous coronary artery dissection predominantly affect women and share common triggers. This case highlights the often misdiagnosed association and emphasises the specific diagnosis and treatment nuances associated with it., Competing Interests: Conflicts of interest None declared., (Copyright © 2024 Medinews (Cardiology) Limited.)
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- 2024
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18. Erratum for Andrade et al., "Antimicrobial Resistance among Leprosy Patients in Brazil: Real-World Data Based on the National Surveillance Plan".
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Andrade ESN, Brandão JG, Silva JSD, Coriolano CRF, Rosa PS, Moraes MO, Ferreira CO, Gomes CM, and Araújo WN
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- 2023
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19. Correction: Association of NOD2 and IFNG single nucleotide polymorphisms with leprosy in the Amazon ethnic admixed population.
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Leturiondo AL, Noronha AB, Mendonça CYR, Ferreira CO, Alvarado-Arnez LE, Manta FSN, Bezerra OCL, Carvalho EF, Moraes MO, Rodrigues FDC, and Talhari C
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[This corrects the article DOI: 10.1371/journal.pntd.0008247.]., (Copyright: © 2023 Leturiondo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2023
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20. Antimicrobial Resistance among Leprosy Patients in Brazil: Real-World Data Based on the National Surveillance Plan.
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Andrade ESN, Brandão JG, Silva JSD, Coriolano CRF, Rosa PS, Moraes MO, Ferreira CO, Gomes CM, and Araújo WN
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- Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Brazil epidemiology, Cross-Sectional Studies, Dapsone therapeutic use, Drug Resistance, Bacterial genetics, Drug Therapy, Combination, Humans, Microbial Sensitivity Tests, Mycobacterium leprae genetics, Recurrence, Rifampin pharmacology, Rifampin therapeutic use, Leprostatic Agents pharmacology, Leprostatic Agents therapeutic use, Leprosy drug therapy, Leprosy epidemiology, Leprosy microbiology
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Brazil ranks second among countries for new cases and first for relapse cases of leprosy worldwide. The Mycobacterium leprae Resistance Surveillance Plan was established. We aimed to present the results of a 2-year follow-up of the National Surveillance Plan in Brazil. A cross-sectional study of leprosy cases was performed to investigate antimicrobial resistance (AMR) in Brazil from October 2018 to September 2020. Molecular screening targeting genes related to dapsone ( folP1 ), rifampin ( rpoB ), and ofloxacin resistance ( gyrA ) was performed. During the referral period, 63,520 active leprosy patients were registered in Brazil, and 1,183 fulfilled the inclusion criteria for molecular AMR investigation. In total, only 16 (1.4%) patients had genetic polymorphisms associated with AMR. Of these, 8 (50%) had cases of leprosy relapse, 7 (43.8%) had cases of suspected therapeutic failure with standard treatment, and 1 (6.2%) was a case of new leprosy presentation. M. leprae strains with AMR-associated mutations were found for all three genes screened. Isolates from two patients showed simultaneous resistance to dapsone and rifampin, indicating multidrug resistance (MDR). No significant relationship between clinical variables and the presence of AMR was identified. Our study revealed a low frequency of AMR in Brazil. Isolates were resistant mainly to dapsone, and a very low number of isolates were resistant to rifampin, the main bactericidal agent for leprosy, or presented MDR, reinforcing the importance of the standard World Health Organization multidrug therapy. The greater frequency of AMR among relapsed patients supports the need to constantly monitor this group.
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- 2022
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21. Diabetic ketoacidosis and COVID-19: what have we learned so far?
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de Sá-Ferreira CO, da Costa CHM, Guimarães JCW, Sampaio NS, Silva LML, de Mascarenhas LP, Rodrigues NG, Dos Santos TL, Campos S, and Young EC
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- Blood Glucose analysis, Blood Glucose Self-Monitoring, COVID-19 metabolism, Diabetes Mellitus, Type 1 complications, Diabetes Mellitus, Type 1 drug therapy, Diabetes Mellitus, Type 1 metabolism, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 metabolism, Diabetic Ketoacidosis drug therapy, Diabetic Ketoacidosis metabolism, Humans, Hyperglycemia complications, Hyperglycemia drug therapy, Hypoglycemic Agents therapeutic use, Insulin administration & dosage, Insulin therapeutic use, Prognosis, Risk Factors, Telemedicine, COVID-19 complications, Diabetic Ketoacidosis complications
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In December 2019, a pandemic emerged due to a new coronavirus that imposed various uncertainties and discoveries. It has been reported that diabetes is a risk factor for worst outcomes of COVID-19 and also that SARS-CoV-2 infection was correlated with the occurrence of diabetic ketoacidosis (DKA) in patients. The aim of this work is to discuss this correlation emphasizing the main case reports from 2020 while exploring the management of DKA during the course of COVID-19. Web of Science, PubMed, and Scopus databases were searched using two sets of Medical Subject Heading (MeSH) search terms or Title/Abstract words: Coronavirus Infections (Coronavirus Infections, Middle East Respiratory Syndrome, COVID-19) and Diabetic Ketoacidosis (Diabetic Ketoacidosis, Diabetic Acidosis, Diabetic Ketosis). There is a clear correlation between COVID-19 and DKA. The SARS-Cov-2 infection may precipitate both a hyperglycemic state and ketoacidosis occurrence in patients with diabetes and nondiabetic patients, which may lead to fatal outcomes. DKA in patients with COVID-19 may increase risk and worse outcomes. Hence, the SARS-Cov-2 infection presents a new perspective toward the management of glycemia and acidosis in patients with diabetes and nondiabetic patients, highlighting the need for rapid interventions to minimize the complications from COVID-19 while reducing its spreading.
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- 2022
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22. Putative pathogen-selected polymorphisms in the PKLR gene are associated with mycobacterial susceptibility in Brazilian and African populations.
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Bezerra OCL, Alvarado-Arnez LE, Mabunda N, Salomé G, de Sousa A, Kehdy FSG, Sales-Marques C, Manta FSN, Andrade RM, Ferreira LP, Leal-Calvo T, Cardoso CC, Nunes K, Gouveia MH, Mbulaiteve SM, Yeboah ED, Hsing A, Latini ACP, Leturiondo AL, Rodrigues FDC, Noronha AB, Ferreira CO, Talhari C, Rêgo JL, Castellucci LCC, Tarazona-Santos E, Carvalho EF, Meyer D, Pinheiro RO, Jani IV, Pacheco AG, and Moraes MO
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- Adult, Brazil, Case-Control Studies, Female, Gene Frequency, Genetic Predisposition to Disease, Haplotypes, Humans, Linkage Disequilibrium, Logistic Models, Male, Middle Aged, Mozambique, Pyruvate Kinase deficiency, Young Adult, Malaria genetics, Polymorphism, Single Nucleotide, Pyruvate Kinase genetics
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Pyruvate kinase (PK), encoded by the PKLR gene, is a key player in glycolysis controlling the integrity of erythrocytes. Due to Plasmodium selection, mutations for PK deficiency, which leads to hemolytic anemia, are associated with resistance to malaria in sub-Saharan Africa and with susceptibility to intracellular pathogens in experimental models. In this case-control study, we enrolled 4,555 individuals and investigated whether PKLR single nucleotide polymorphisms (SNPs) putatively selected for malaria resistance are associated with susceptibility to leprosy across Brazil (Manaus-North; Salvador-Northeast; Rondonópolis-Midwest and Rio de Janeiro-Southeast) and with tuberculosis in Mozambique. Haplotype T/G/G (rs1052176/rs4971072/rs11264359) was associated with leprosy susceptibility in Rio de Janeiro (OR = 2.46, p = 0.00001) and Salvador (OR = 1.57, p = 0.04), and with tuberculosis in Mozambique (OR = 1.52, p = 0.07). This haplotype downregulates PKLR expression in nerve and skin, accordingly to GTEx, and might subtly modulate ferritin and haptoglobin levels in serum. Furthermore, we observed genetic signatures of positive selection in the HCN3 gene (xpEHH>2 -recent selection) in Europe but not in Africa, involving 6 SNPs which are PKLR/HCN3 eQTLs. However, this evidence was not corroborated by the other tests (FST, Tajima's D and iHS). Altogether, we provide evidence that a common PKLR locus in Africans contribute to mycobacterial susceptibility in African descent populations and also highlight, for first, PKLR as a susceptibility gene for leprosy and TB., Competing Interests: The authors have declared that no competing interests exist. Author Edward D. Yeboah was unavailable to confirm their authorship contributions. On their behalf, the corresponding author has reported their contributions to the best of their knowledge.
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- 2021
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23. New Insights Into Peptide Cannabinoids: Structure, Biosynthesis and Signaling.
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Riquelme-Sandoval A, de Sá-Ferreira CO, Miyakoshi LM, and Hedin-Pereira C
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Classically, the endocannabinoid system (ECS) consists of endogenous lipids, of which the best known are anandamide (AEA) and 2 arachidonoylglycerol (2-AG), their enzyme machinery for synthesis and degradation and their specific receptors, cannabinoid receptor one (CB1) and cannabinoid receptor two (CB2). However, endocannabinoids also bind to other groups of receptors. Furthermore, another group of lipids are considered to be endocannabinoids, such as the fatty acid ethanolamides, the fatty acid primary amides and the monoacylglycerol related molecules. Recently, it has been shown that the hemopressin peptide family, derived from α and β chains of hemoglobins, is a new family of cannabinoids. Some studies indicate that hemopressin peptides are expressed in the central nervous system and peripheral tissues and act as ligands of these receptors, thus suggesting that they play a physiological role. In this review, we examine new evidence on lipid endocannabinoids, cannabinoid receptors and the modulation of their signaling pathways. We focus our discussion on the current knowledge of the pharmacological effects, the biosynthesis of the peptide cannabinoids and the new insights on the activation and modulation of cannabinoid receptors by these peptides. The novel peptide compounds derived from hemoglobin chains and their non-classical activation of cannabinoid receptors are only starting to be uncovered. It will be exciting to follow the ensuing discoveries, not only in reference to what is already known of the classical lipid endocannabinoids revealing more complex aspects of endocannabinoid system, but also as to its possibilities as a future therapeutic tool., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Riquelme-Sandoval, de Sá-Ferreira, Miyakoshi and Hedin-Pereira.)
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- 2020
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24. Development of a Statistical Workflow for Screening Protein Extracts Based on their Nutritional Composition and Digestibility: Application to Elderly.
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Duval A, Sayd T, Aubry L, Ferreira CO, Ferraro V, and Sante-Lhoutellier V
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The objective of the study is to develop a workflow to screen protein extracts and identify their nutritional potential as high quality nutritional culinary aids for recipes for the elderly. Twenty-seven protein extracts of animal, vegetable, and dairy origin were characterized. We studied their fate by monitoring static in vitro digestion, mimicking the physiological digestion conditions of the elderly. At the end of the gastric and intestinal phase, global measurements of digestibility and antioxidant bioactivities were performed. The statistical analysis workflow developed allowed: (i) synthesizing the compositional and nutritional information of each protein extract by creating latent variables, and (ii) comparing them. The links between variables and similarities between protein extracts were visualized using a heat map. A hierarchical cluster analysis allowed reducing the 48 quantitative variables into 15 qualitative latent variables (clusters). The application of the k-means method on each cluster enable to classify the protein extracts by level. This defined level was used as categorical value. Multiple correspondence analysis revealed groups of protein extracts with varied patterns. This workflow allowed the comparison/hierarchization between protein extracts and the creation of a tool to select the most interesting ones on the basis of their nutritional quality.
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- 2020
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25. Association of NOD2 and IFNG single nucleotide polymorphisms with leprosy in the Amazon ethnic admixed population.
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Leturiondo AL, Noronha AB, Mendonça CYR, Ferreira CO, Alvarado-Arnez LE, Manta FSN, Bezerra OCL, Carvalho EF, Moraes MO, Rodrigues FDC, and Talhari C
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- Adult, Aged, Brazil, Case-Control Studies, Female, Genotyping Techniques, Humans, Male, Middle Aged, Young Adult, Genetic Predisposition to Disease, Interferon-gamma genetics, Leprosy genetics, Nod2 Signaling Adaptor Protein genetics, Polymorphism, Single Nucleotide
- Abstract
Leprosy is a chronic infectious disease, caused by Mycobacterium leprae, which affects skin and peripheral nerves. Polymorphisms in genes associated with autophagy, metabolism, innate and adaptive immunity confer susceptibility to leprosy. However, these associations need to be confirmed through independent replication studies in different ethnicities. The population from Amazon state (northern Brazil) is admixed and it contains the highest proportion of Native American genetic ancestry in Brazil. We conducted a case-control study for leprosy in which we tested fourteen previously associated SNPs in key immune response regulating genes: TLR1 (rs4833095), NOD2 (rs751271, rs8057341), TNF (rs1800629), IL10 (rs1800871), CCDC122/LACC1 (rs4942254), PACRG/PRKN (rs9356058, rs1040079), IFNG (rs2430561), IL6 (rs2069845), LRRK2 (rs7298930, rs3761863), IL23R (rs76418789) and TYK2 (rs55882956). Genotyping was carried out by allelic discrimination in 967 controls and 412 leprosy patients. Association with susceptibility was assessed by logistic regression analyses adjusted for the following covariates: gender, age and ancestry. Genetic ancestry was similar in case and control groups. Statistically significant results were only found for IFNG and NOD2. The rs8057341 polymorphism within NOD2 was identified as significant for the AA genotype (OR = 0.56; 95% CI, 0.37-0.84; P = 0.005) and borderline for the A allele (OR = 0.76; 95% CI, 0.58-1.00; P = 0.053) and carrier (OR = 0.76; 95% CI, 0.58-1.00; P = 0.051). The rs2430561 SNP in IFNG was associated with disease susceptibility for the AT genotype (OR = 1.40; 95% CI, 1.06-1.85; P = 0.018) and carrier (OR = 1.44; 95% CI, 1.10-1.88; P = 0.008). We confirmed that NOD2 and IFNG are major players in immunity against M.leprae in the Amazon ethnic admixed population., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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26. Amino acid permeases in Cryptococcus neoformans are required for high temperature growth and virulence; and are regulated by Ras signaling.
- Author
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Calvete CL, Martho KF, Felizardo G, Paes A, Nunes JM, Ferreira CO, Vallim MA, and Pascon RC
- Subjects
- Amino Acid Sequence, Amino Acid Transport Systems chemistry, Amino Acid Transport Systems genetics, Amino Acids metabolism, Cryptococcus neoformans growth & development, Fungal Proteins chemistry, Fungal Proteins genetics, Mutagenesis, Protein Isoforms genetics, Protein Isoforms metabolism, Sequence Alignment, Stress, Physiological, Temperature, ras Proteins genetics, Amino Acid Transport Systems metabolism, Cryptococcus neoformans physiology, Fungal Proteins metabolism, Signal Transduction, Virulence genetics, ras Proteins metabolism
- Abstract
Cryptococcosis is an Invasive Fungal Infection (IFI) caused by Cryptococcus neoformans, mainly in immunocompromised patients. Therapeutic failure due to pathogen drug resistance, treatment inconstancy and few antifungal options is a problem. The study of amino acid biosynthesis and uptake represents an opportunity to explore possible development of novel antifungals. C. neoformans has 10 amino acids permeases, two of them (Aap3 and Aap7) not expressed at the conditions tested, and five were studied previously (Aap2, Aap4, Aap5, Mup1 and Mup3). Our previous results showed that Aap4 and Aap5 are major permeases with overlapping functions. The aap4Δ/aap5Δ double mutant fails to grow in amino acids as sole nitrogen source and is avirulent in animal model. Here, we deleted the remaining amino acid permeases (AAP1, AAP6, AAP8) that showed gene expression modulation by nutritional condition and created a double mutant (aap1Δ/aap2Δ). We studied the virulence attributes of these mutants and explored the regulatory mechanism behind amino acid uptake in C. neoformans. The aap1Δ/aap2Δ strain had reduced growth at 37°C in L-amino acids, reduced capsule production and was hypovirulent in the Galleria mellonella animal model. Our data, along with previous studies, (i) complement the analysis for all 10 amino acid permeases mutants, (ii) corroborate the idea that these transporters behave as global permeases, (iii) are required during heat and nutritional stress, and (iv) are important for virulence. Our study also indicates a new possible link between Ras1 signaling and amino acids uptake., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
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27. Performance of serological tests PGL1 and NDO-LID in the diagnosis of leprosy in a reference Center in Brazil.
- Author
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Leturiondo AL, Noronha AB, do Nascimento MOO, Ferreira CO, Rodrigues FDC, Moraes MO, and Talhari C
- Subjects
- Adolescent, Adult, Aged, Antibodies, Bacterial blood, Antibodies, Bacterial immunology, Brazil, Case-Control Studies, Child, Early Diagnosis, Female, Humans, Leprosy blood, Leprosy, Multibacillary diagnosis, Leprosy, Paucibacillary diagnosis, Male, Middle Aged, Mycobacterium leprae immunology, Sensitivity and Specificity, Antigens, Bacterial immunology, Glycolipids immunology, Leprosy diagnosis, Serologic Tests methods
- Abstract
Background: Early detection of leprosy and multidrug therapy are crucial to achieve zero transmission and zero grade II incapacities goals of World Health Organization. Leprosy is difficult to diagnose because clinical forms vary and there are no gold standard methods to guide clinicians. The serological rapid tests aid the clinical diagnosis and are available for field use. They are easy to perform, do not require special equipment or refrigeration and are cheaper than the molecular tests., Methods: We evaluated the performance of two rapid serological tests (PGL1 and NDO-LID) in the discrimination of leprosy cases from healthy individuals at the Alfredo da Matta Foundation, a reference center for the disease in Manaus, Amazonas, Brazil. PGL1 and NDO-LID rapid tests are capable of detecting specific antibodies of M. leprae, IgM and IgM/IgG, respectively. A total of 530 healthy subjects and 171 patients (50 with paucibacillary and 121 multibacillary leprosy) were included in the study., Results: Among the paucibacillary leprosy patients, the sensitivity was 34.0 and 32.0% for the NDO-LID and PGL1, respectively. In multibacillary leprosy patients, the NDO-LID sensitivity was 73.6% and the PGL1 was 81.0%. Serological tests demonstrated specificities of 75.9% for PGL-1 and 81.7% for NDO-LID. The positive predictive value (PPV), negative predictive value (NPV) and accuracy in multibacillary patients were 47.9, 93.1, and 80.2% respectively for the NDO-LID, and 43.4, 94.6 76.8% for PGL1., Conclusions: The tests showed limited capacity in the diagnosis of the disease, however, the high negative predictive value of the tests indicates a greater chance of true negatives in this group favoring exclusion of leprosy. This characteristic of the ML flow test is important in aiding clinical Diagnosis, especially in a region endemic to the disease and with other confounding skin conditions.
- Published
- 2019
- Full Text
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28. Development, validation and testing costs of an in-house real-time PCR assay for the detection of Chlamydia trachomatis.
- Author
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Santos CGD, Sabidó M, Leturiondo AL, Ferreira CO, da Cruz TP, and Benzaken AS
- Subjects
- Adolescent, Adult, Asymptomatic Infections, Brazil, Cervix Uteri microbiology, Chlamydia Infections microbiology, Chlamydia trachomatis isolation & purification, DNA Primers, DNA, Bacterial genetics, Female, Humans, Plasmids, Reagent Kits, Diagnostic, Real-Time Polymerase Chain Reaction standards, Sensitivity and Specificity, Young Adult, Chlamydia Infections diagnosis, Chlamydia trachomatis genetics, Real-Time Polymerase Chain Reaction economics, Real-Time Polymerase Chain Reaction methods
- Abstract
Purpose: To improve the screening of Chlamydia trachomatis(C. trachomatis) in Brazil, an accurate and affordable method is needed. The objective of this study was to develop and assess the performance and costs of a new in-house real-time PCR (qPCR) assay for the diagnosis of C. trachomatis infection., Methodology: Asymptomatic women aged 14-25 years who attended primary health services in Manaus, Brazil, were screened for C. trachomatis using the Digene Hybrid Capture II CT-ID (HCII CT-ID) DNA test. A subset of cervical specimens were tested using an in-house qPCR and a commercial qPCR, ArtusC. trachomatis Plus RG PCR 96 CE (Artus qPCR) kit, as a reference test. A primer/probe based on the sequence of cryptic plasmid (CP) was designed. An economic evaluation was conducted from the provider's perspective., Results: The primers were considered specific for C. trachomatis because they did not amplify any product from non-sexually transmitted bacterial species tested. Overall, 292 specimens were tested by both the commercial kit (Artus qPCR) and the in-house qPCR. Of those, one resulted in no amplification and was excluded from the analysis. The sensitivity, specificity, and positive and negative predictive values of the in-house qPCR were 99.5 % [95 % confidence interval (CI): 97.1-100], 95.1 % (95 % CI: 89-98.4), 97.4 % (95 % CI: 94-99.1) and 99.0 % (95 % CI: 94.5-100), respectively. The cost per case of C. trachomatis was £0.44 ($0.55) for HCII CT-ID, £1.16 ($1.45) for Artus qPCR and £1.06 ($1.33) for in-house qPCR., Conclusion: We have standardized an in-house qPCR to detect cervical C. trachomatis targeting CP. The in-house qPCR showed excellent accuracy and was more affordable than the commercial qPCR kit.
- Published
- 2017
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29. Biocompatibility of a calcium hydroxide-propolis experimental paste in rat subcutaneous tissue.
- Author
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Mori GG, Rodrigues Sda S, Shibayama ST, Pomini M, and do Amaral CO
- Subjects
- Animals, Male, Rats, Rats, Wistar, Biocompatible Materials, Calcium Hydroxide chemistry, Dental Materials, Propolis, Subcutaneous Tissue
- Abstract
Intracanal medications are fundamental for disinfection of the root canal system and participate in periapical repair, so their biocompatibility is of utmost importance to avoid tissue damage. This study evaluated the biocompatibility of a experimental paste of calcium hydroxide and propolis in the subcutaneous tissue of rats. The study was conducted on 15 male Wistar rats. Two incisions were made on the dorsal region of each animal for introduction of 4 tubes: one tube was empty; one contained zinc oxide-eugenol cement, and the two other tubes were filled with experimental paste. After 7, 14 and 30 days, the animals were euthanized and the specimens were subjected to histotechnical preparation. The hematoxylin and eosin-stained histological sections were analyzed by light microscopy. Scores were established according to the inflammatory process and statistically compared by the Tukey test (α = 5%). The analysis of histological sections showed non-significant or mild inflammatory reaction in the connective tissue in contact with the empty tubes in all study periods while the contact of subcutaneous tissue with zinc oxide-eugenol elicited moderate or severe inflammation similarly without significant difference among the study periods. The connective tissue was moderately inflamed at 7 days when contacting the experimental paste, but the inflammatory process was non-significant or mild at 14 and 30 days. The experimental paste was biocompatible with the tissues after 14 days of subcutaneous implantation.
- Published
- 2014
- Full Text
- View/download PDF
30. Severe Plasmodium vivax malaria, Brazilian Amazon.
- Author
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Alexandre MA, Ferreira CO, Siqueira AM, Magalhães BL, Mourão MP, Lacerda MV, and Alecrim Md
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antimalarials therapeutic use, Brazil epidemiology, Child, Child, Preschool, Chloroquine therapeutic use, Female, Humans, Infant, Infant, Newborn, Malaria, Vivax drug therapy, Malaria, Vivax parasitology, Male, Middle Aged, Plasmodium vivax classification, Plasmodium vivax drug effects, Plasmodium vivax genetics, Polymerase Chain Reaction methods, Primaquine therapeutic use, Severity of Illness Index, Young Adult, Malaria, Vivax epidemiology, Malaria, Vivax physiopathology, Plasmodium vivax isolation & purification
- Abstract
We describe a case series of 17 patients hospitalized in Manaus (western Brazilian Amazon) with PCR-confirmed Plasmodium vivax infection who were treated with chloroquine and primaquine. The major complications were jaundice and severe anemia. No in vivo chloroquine resistance was detected. These data help characterize the clinical profile of severe P. vivax malaria in Latin America.
- Published
- 2010
- Full Text
- View/download PDF
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