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Your search keyword '"Fiorillo, A.S."' showing total 45 results
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45 results on '"Fiorillo, A.S."'

44. Influence of the Fabrication Accuracy of Hot-Embossed PCL Scaffolds on Cell Growths

Author

Enzo Di Fabrizio, Cirino Botta, E. Lamanna, Tania Limongi, Antonino S. Fiorillo, Bernadette Scopacasa, Maria Laura Coluccio, Pierfrancesco Tassone, Pierosandro Tagliaferri, Costantino Davide Critello, Patrizio Candeloro, Elisabetta Dattola, Gerardo Perozziello, Maria Cucè, Maria Eugenia Gallo Cantafio, Salvatore A. Pullano, Limongi T., Dattola E., Botta C., Coluccio M.L., Candeloro P., Cuce M., Scopacasa B., Cantafio M.E.G., Critello C.D., Pullano S.A., Fiorillo A.S., Tagliaferri P., Tassone P., Lamanna E., Di Fabrizio E., and Perozziello G.

Subjects
0301 basic medicine, Scaffold, Histology, Fabrication, Materials science, Scanning electron microscope, lcsh:Biotechnology, Biomedical Engineering, Bioengineering, 02 engineering and technology, demolding force, medicine.disease_cause, Microstructured scaffold, 03 medical and health sciences, chemistry.chemical_compound, microstructured scaffolds, Tissue engineering, polycaprolactone, Mold, lcsh:TP248.13-248.65, medicine, Cell viabilityC, cell viability, Original Research, Bioengineering and Biotechnology, 021001 nanoscience & nanotechnology, Microstructure, Biodegradable polymer, 030104 developmental biology, chemistry, hot embossing, Polycaprolactone, 0210 nano-technology, Cell viability, Demolding force, Hot embossing, Microstructured scaffolds, Biotechnology, Biomedical engineering
Abstract

Polycaprolactone (PCL) is a biocompatible and biodegradable polymer widely used for the realization of 3D scaffold for tissue engineering applications. The hot embossing technique (HE) allows the obtainment of PCL scaffolds with a regular array of micro pillars on their surface. The main drawback affecting this kind of micro fabrication process is that such structural superficial details can be damaged when detaching the replica from the mold. Therefore, the present study has focused on the optimization of the HE processes through the development of an analytical model for the prediction of the demolding force as a function of temperature. This model allowed calculating the minimum demolding force to obtain regular micropillars without defects. We demonstrated that the results obtained by the analytical model agree with the experimental data. To address the importance of controlling accurately the fabricated microstructures, we seeded on the PCL scaffolds human stromal cell line (HS-5) and monocytic leukemia cell line (THP-1) to evaluate how the presence of regular or deformed pillars affect cells viability. In vitro viability results, scanning electron and fluorescence microscope imaging analysis show that the HS-5 preferentially grows on regular microstructured surfaces, while the THP-1 on irregular microstructured ones.

Published
2020

45. Development of a Low-cost Nailfold Capillaroscopy Platform to Enhance Early Detection of Secondary Raynaud's Phenomenon

Author

Maria Giovanna Bianco, Francesco Ursini, Ifana Mahbub, Nishat T. Tasneem, Salvatore A. Pullano, Antonino S. Fiorillo, Caterina Bruno, Pullano S.A., Bianco M.G., Ursini F., Bruno C., Fiorillo A.S., Mahbub I., and Tasneem N.T.

Subjects
030203 arthritis & rheumatology, 0301 basic medicine, Secondary Raynaud's Phenomenon, medicine.medical_specialty, Computer science, Hospital setting, Early detection, Distributed Measurement Station, Automated Measurement, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Physical medicine and rehabilitation, Rheumatology, medicine, Nailfold Capillaroscopy
Abstract

Nailfold Video Capillaroscopy (NVC) is a simple and powerful tool used in rheumatology to recognize morphological and/or functional abnormalities in the microcirculation. Despite its key role in the early diagnosis of Systemic Sclerosis (SSc), its diffusion is confined to central public hospitals. In this paper, the design and fabrication of a low-cost NVC platform are presented, which enables a distributed measuring station for improving the diffusion of this technique in a non-hospital setting. An automated measuring system is implemented for the evaluation of quantitative NVC parameters assisting in the interpretation of a non-specialist. Comparison between the scores obtained from a commercial capillaroscope and the proposed device showed an overall good agreement.

Published
2018

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