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The apple crop, integral to global agriculture, is facing a significant challenge in the form of mosaic disease, reducing productivity, particularly in temperate regions of the world. The intricate biochemical and physiological responses of apple genotypes to mosaic infection induced by apple mosaic virus (ApMV)/apple necrotic mosaic virus (ApNMV) were investigated in this study. Higher phenolic contents, DPPH, flavonoids and flavanols were recorded from mosaic-infected apple genotypes in comparison with their respective counterparts. The impact on carbohydrates/total starch and chlorophyll content revealed a substantial reduction in mosaic-infected as compared to healthy leaves, which emerges as a quantitative biomarker for measuring infection severity and offering insights into the compromised photosynthetic ability of infected leaves. In mosaic-infected genotypes, PAL activity, malondialdehyde and antioxidant enzymes were greater, strengthening the powerful antioxidative defense mechanism against mosaic infection. Principal component analysis provided a comprehensive overview, identifying key biomarkers and elucidating the underlying biochemical processes linked with apple mosaic disease. This research provides significant insights for future apple breeding programs by providing a more detailed knowledge of the biochemical intricacies underpinning plant defense systems against mosaic infection in apples. [ABSTRACT FROM AUTHOR]

BACKGROUND RESULTS CONCLUSION Tea is the second most consumed beverage worldwide and its health benefits have been extensively studied because of its rich flavanol content. This study aims to evaluate the efficacy of a novel continuous step‐wise temperature extraction process in maximising antioxidant activity, total phenolic content and flavanol concentration of different tea beverages.Continuous step‐wise temperature extraction produced the highest absolute antioxidant capacity, total phenolic content and concentration of major tea flavanols in Yuhua tea compared to the other tea types at all extraction points. Despite having the lowest baseline concentration of tea flavanols, Lapsang Souchong tea showed the greatest increase in catechin and epigallocatechin‐3‐gallate levels with the continuous step‐down temperature process, by over 500% and 300%, respectively. In addition, Moonlight White tea showed the greatest percentage change in total antioxidant capacity and phenolic content at the end of the process compared to the baseline. Furthermore, the continuous step‐wise temperature extraction showed a moderate increase in caffeine concentration in all the tea samples.Our findings revealed a beneficial impact of continuous step‐wise temperature extraction on the antioxidant capacity, phenolic content and flavanol profile in different types of tea, which may be valuable in the production of added‐value tea beverages. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Gulf War Illness (GWI) afflicts US military personnel who served in the Persian Gulf War. Suspect causal agents include exposure to pyridostigmine (PB), permethrin (PM) and N,N-diethyl-m-toluamide (DEET). Prominent symptoms include cognitive deficits, such as memory impairment. In aging animal models, we have documented the beneficial effect of the flavanol (-)-epicatechin (Epi) on hippocampus structure and related function. Using a rat model of GWI, we examined the effects of Epi on hippocampus inflammation, oxidative stress, mitochondrial dysfunction, cell death/survival pathways, and memory endpoints. Male Wistar rats underwent 3 weeks of exposure to either vehicles or DEET, PM, PB, and stress. Subgroups of GWI rats were then allocated to receive orally 15 days of either water (vehicle) or 1 mg/kg/day of Epi treatment. Object recognition tasks were performed to assess memory. Hippocampus samples were analyzed. Epi treatment yields significant improvements in short- and long-term memory versus GWI rats. Hippocampus oxidative stress and pro-inflammatory cytokine levels showed significant increases with GWI that were largely normalized with Epi becoming comparable to controls. Significant increases in markers of hippocampus neuroinflammation and cell death were noted with GWI and were also largely reduced with Epi. Neuronal survival signaling pathways were adversely impacted by GWI and were partially or fully restored by Epi. Markers of mitochondrial function were adversely impacted by GWI and were fully restored by Epi. In conclusion, in an animal model of GWI, Epi beneficially impacts recognized markers of hippocampus neuroinflammation, oxidative stress, cell survival, neurotoxicity and mitochondrial function leading to improved memory. [ABSTRACT FROM AUTHOR]

This study investigated the change in color of black soybean seed coats during boiling process owing to the reaction between iron ions and polyphenols, especially non-extractable proanthocyanidins (NEPAs). Beans boiled three times in water were discolored to reddish brown, and the content of anthocyanins (cyanidin 3-glucoside) was less than 1 % of that of raw beans; however, they turned jet black when boiled in 5 mmol/L ferrous sulfate solution. Seed coats from which extractable polyphenols such as anthocyanins and low-molecular weight flavanols were completely removed by extraction with the aqueous–organic solvents had a reddish brown color; however, NEPAs remained in them. Therefore, it was considered that the main mechanism by which the black color of the seed coat is maintained using iron agents during boiling process is the reaction of iron ions with NEPAs and not anthocyanins. [ABSTRACT FROM AUTHOR]

Cocoa flavan-3-ols affect endothelium-dependent responses in resistance vessels and microcirculation has received little attention. We tested the effects of dark chocolate consumption (396 mg total flavanols/day for 3 days) in two Groups of 10 men (18–25 years; non-smokers) each comprising equal numbers of White European (WE) and South Asian (SA) ethnicity. In Group 1, dark chocolate did not affect reactive hyperaemia in forearm muscle, but augmented muscle dilatation evoked by acute mental stress, and reactive hyperaemia and acetylcholine (ACh)-evoked dilatation in cutaneous microcirculation. Conversely, in Group 2, chocolate did not affect cutaneous reactive hyperaemia or ACh-evoked dilatation, but these responses were blunted in Group 1 relative to Group 2. Further, when Groups 1 and 2 were combined, responses were blunted in SAs relative to WEs, augmented by chocolate in SAs only. In Group 2 individuals whose ACh-evoked dilatation was attenuated by nitric oxide synthase (NOS) inhibition, ACh-evoked dilatation was not altered after chocolate, but the attenuating effect of NOS inhibition was lost. Conversely, in Group 2 individuals whose ACh-evoked dilatation was enhanced by NOS inhibition, ACh-evoked dilatation was also augmented by chocolate. We propose that in resistance and microvessels of young men, cocoa flavan-3-ols preferentially augment endothelium-dependent dilator responses whose responses are depressed by familial and lifestyle factors more prevalent in SAs than Wes. Flavan-3-ols may facilitate the NOS pathway but also influence other endothelium-dependent dilators. [ABSTRACT FROM AUTHOR]

The objective of the study was to determine the effect of vintage and cultivar on the total polyphenol content and the antioxidant activity of wines made from the white wine cultivar 'Solaris' and the red wine cultivar 'Zilga' (both with skin maceration). The second goal was to describe the polyphenolic profile of 'Solaris' wine and compare it with that of 'Zilga' wine. Spectrophotometric methods were employed to determine the total polyphenol content and the antioxidant activity. High-performance liquid chromatography (HPLC) was used to determine the polyphenol composition. The total polyphenol content and the antioxidant activity of 'Solaris' wine differed significantly from that of 'Zilga' wine in the experimental years from 2021 to 2023. Significant differences between the wines also occurred on average over the years, with a significantly higher total polyphenol content and antioxidant activity obtained for the red wine of 'Zilga'. However, both wines were similarly influenced by vintage over the three-year experimental period. The antioxidant activity showed a high correlation with the total polyphenol content. The polyphenolic profile of 'Solaris' wine differed notably from that of 'Zilga' wine. While 'Solaris' wine exhibited lower levels of phenolic acids and flavonols, the content of the flavanols was significantly higher, even double that of 'Zilga' wine. Among the flavanols, there was a remarkably high content of catechin and epicatechin. [ABSTRACT FROM AUTHOR]

Fructose bisphosphate aldolases (FBAs) catalyze the reversible cleavage of fructose 1,6‐bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3‐phosphate. We analyzed two previously uncharacterized cytosolic Arabidopsis FBAs, AtFBA4 and AtFBA5. Based on a recent report, we examined the interaction of AtFBA4 with calmodulin (CaM)‐like protein 11 (AtCML11). AtFBA4 did not bind AtCML11; however, we found that CaM bound AtFBA5 in a Ca2+‐dependent manner with high specificity and affinity (KD ~ 190 nm) and enhanced its stability. AtFBA4 and AtFBA5 exhibited Michaelis–Menten kinetics with Km and Vmax values of 180 μm and 4.9 U·mg−1 for AtFBA4, and 6.0 μm and 0.30 U·mg−1 for AtFBA5, respectively. The flavonoid morin inhibited both isozymes. Our study suggests that Ca2+ signaling and flavanols may influence plant glycolysis/gluconeogenesis. [ABSTRACT FROM AUTHOR]

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Stanozolol, an anabolic steroid, has been shown to induce kidney damage through mechanisms involving oxidative stress and inflammation. Natural Cocoa Powder (NCP), known for its high antioxidant content, may offer protective effects against such damage. Aim: This study aimed to evaluate the potential of Natural Cocoa Powder (NCP) in ameliorating kidney damage caused by stanozolol intramuscular injections in rats. Methods: Thirty rats were randomly assigned into five groups (G1–G5) and fed with standard rat chow. G1 and G3 received 2 mg/kg stanozolol intramuscularly twice weekly, G2 and G4 received 5 mg/kg stanozolol twice weekly, while G5 served as the control with no stanozolol. G1, G2, and G5 had 24 h access to water, while G3 and G4 had water replaced with 2 % NCP from 6 am to 6 pm daily. After 8 weeks, kidneys were perfusion-fixed and analyzed histomorphometrically for proximal/distal tubules, Bowman's space, glomerular tuft, and renal corpuscle composition.Before and after treatments, renal function was measured by serum creatinine and blood urea nitrogen, whilst systemic inflammation was monitored by erythrocyte sedimentation rate and serum C-reactive protein tests. Results and Discussion: Stanozolol administration significantly increased kidney damage in a dose-dependent manner, with marked rises in the volume densities of the proximal convoluted tubule (PCT), distal convoluted tubule (DCT), glomerular tuft, and renal corpuscle. At higher doses, PCT and DCT volume densities increased by 143 % and 223 %, respectively, compared to controls. Natural cocoa powder (NCP) mitigated these effects, reducing PCT, DCT, and glomerular tuft volume densities by 92 %, 77 %, and 86 %, respectively. Stanozolol also elevated serum markers of kidney dysfunction and inflammation, such as blood urea nitrogen (BUN), serum creatinine (SCr), and C-reactive protein (CRP). While NCP significantly lowered BUN and SCr levels, it did not fully normalize CRP, which remained elevated in stanozolol-treated rats. Conclusion: Increased volume density of kidney components indicated hypertrophy, which was associated with elevated serum creatinine, blood urea nitrogen (BUN), and C-reactive protein (CRP) levels, reflecting impaired renal function and heightened systemic inflammation. The reduction in these markers with NCP ingestion suggests its potential to mitigate stanozolol-induced renal damage. It is proposed that the antioxidant properties of NCP may have reduced inflammation by counteracting oxidative stress, thereby contributing to the observed improvement in kidney function and structure.

Studies have shown that natural flavone and flavonoids are molecules derived from the natural source and can selectively kill certain cancer cells. However, the exact process by which these bioactive substances affect acute myeloid leukemia is still unknown. To further understand about the medication resistance and find possible treatments for pediatric acute myeloid leukemia, we used network pharmacology methods. We specifically examined the pharmacological impacts of polyphenols, particularly kaempferol, catechin and quercetin on the treatment of acute myeloid leukemia. Using public databases including PubChem, Kyoto Encyclopedia of Genes and Genomes Mapper, Universal Protein Resource, DisGenet and cystoscope databases, we conducted a thorough search for the potential targets of polyphenols and acute myeloid leukemia. Consequently, we identified polyphenol target genes and acute myeloid leukemia disease targets. Among the 253 genes showed overlapping; however, some might be potential as polyphenol-based acute myeloid leukemia therapy targets. The specific method by which these bioactive substances exert their anti-acute myeloid leukemia effects has been elucidated through the use of protein-protein interaction network analysis, gene ontology analysis and Kyoto Encyclopaedia of Genes and Genomes pathway enrichment analysis. Further cytological examinations confirmed these results, illustrating the polyphenol's capacity to hinder the growth of acute myeloid leukemia cells through different pathways. Our study combines network pharmacology-based predictions with systematic review studies for polyphenolic-mediated therapy of acute myeloid leukemia. This provides new perspectives on therapeutic approaches to address drug resistance in pediatric acute myeloid leukemia. [ABSTRACT FROM AUTHOR]

Studies indicated that cocoa-based products effectively mitigate the risks associated with metabolic syndrome (MetS), however, the effect varies based on cocoa types, dosages, and study durations. This review aimed to determine the flavanol-rich cocoa consumption on MetS outcomes within the last decade (2013-2023), adhering to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Seven randomized-controlled trials (RCTs) used cocoa-based products containing 0.3-1680 mg flavanol monomers and 3.5-1270 mg procyanidins. Cocoa-based products beneficially reduced glycemic response, blood pressure and lipid profiles. However, this review highlights little evidence pinpointing the best cocoa products type and required dosage for the observed effects. Further intervention aiming to improve MetS should justify the selection and concentration of flavanols (monomers and procyanidins). A robust study design should consider registering the trials before study commencement, consider multicenter RCT trials, and adjust for potential covariates that might "masked" the outcomes. [ABSTRACT FROM AUTHOR]

Flavonoids are a diverse group of naturally occurring plant compounds that are primarily composed of phenols in their free form or glycosides. Flavonoid medicines are primarily composed of 2‐phenyl‐g‐benzopyrone. This family consists of monomeric flavanols, flavanones, anthocyanidins, flavones, isoflavonoids, and flavonols. The study's main objective is to review the newly discovered natural flavones and flavanols, as well as the biological and pharmacological actions of these essential flavonoids. As per the available data, 3‐hydroxy flavone plays a vital role in the prevention of a range of diseases. Research studies have confirmed that 3‐hydroxyflavone has a broad range of pharmacological properties like therapeutic effects against pain, inflammation, oxidative stress; hepatotoxicity, hypertension, ulcerogenesis, allergy, platelet dysfunction, microbes, fungi, viruses, rhinovirus, malaria, and carcinogens. 3‐Hydroxy flavone has limited utility because of their poor solubility and bioavailability. This has led to the search for new drug delivery methods, such as nanoparticles. To get over these problems, it would be preferable to administer 3‐hydroxyflavone via the biocompatible nanoparticles. It is expected that nanoparticles will improve the solubility of 3‐hydroxyflavone, enhance its therapeutic properties and facilitate its transport to the brain. [ABSTRACT FROM AUTHOR]

Grapes are a rich source of phenolic compounds with high antioxidant, antibacterial, and nutritional properties among fruits. The aim of this study was to investigate different classes of phenolic compounds in the berry skin of eleven Vitis vinifera cultivars. The phenolic compounds were flavonols, flavanols, flavone, anthocyanins, stilbenes, and phenolic acids. The highest amounts of catechin, epicatechin, ferulic acid, and chlorogenic acid were observed in 'Yaghooti' grape cultivar (P≤0.05). However, the amounts of catechin gallat, kaempferol, myricetin, and pcoumaric acid in 'Bidaneh Ghermez' berries were higher (P≤0.05) compared to other cultivars. Quercetin was the main flavonol and was highest (9.48 μg g-1; P≤0.05) in 'Yaghooti' berries. Luteolin content, as a flavone, ranged from 0.49 μmol g-1 in 'Rishbaba' berry skin to 0.88 μmol g-1 in 'Bidaneh Ghermez'. Delphinidin-3-glucoside and malvidin-3-glucoside were highest in 'Yaghooti'. Cyanidin-3-glucoside and peonidin -3-glucoside were highest in 'Angoor Siah.' Petunidin 3-glucoside was highest in 'Bidaneh Ghermez' (P≤0.05). Berry skin resveratrol varied from 22.7 μg g-1 in 'Monaqa and Fakhri' cultivars to 54.8 μg g-1 FW in 'Bidaneh Ghermez,' with an overall average of 36.9 μg g-1 FW. Among different cultivars, the antioxidant capacity of 'Angoor Siah' was highest (71.3%; P≤0.05) and 'Monaqa' was lowest. The 'Angoor Siah' cultivar had more antibacterial activity compared to other cultivars. In sum, the berry skin of 'Yaghooti,' 'Angoor Siah,' and 'Bidaneh Ghermez' showed the highest health-promoting bioactive compounds, potentially important for future studies. [ABSTRACT FROM AUTHOR]

This study investigates the effectiveness of Kaempferol, a compound extracted from broccoli (Brassica oleracea var. italica), as a catalyst in controlling melanin production through its interaction with the tyrosinase protein. Utilizing a variety of computational tools, including Pymol, Pyrex, Protein Plus, and Lepinski Rule software, the study explores Kaempferol's binding affinity with tyrosinase and its molecular properties. The findings reveal Kaempferol's notable binding scores with tyrosinase and its structural stability as indicated by RMSD analysis. Furthermore, Protein Plus software confirms the interaction between Kaempferol and tyrosinase protein. Lipinski's analysis of Kaempferol shows it meets key pharmacokinetic properties like molecular weight, hydrogen bond formation potential, and reactivity. These insights establish a preliminary understanding of Kaempferol's role as a catalyst in melanin synthesis regulation, suggesting its potential application in treating skin pigment disorders. [ABSTRACT FROM AUTHOR]

IntroductionThis study aimed to evaluate the color and the discriminating compounds for two types of cocoa beans (black and brown beans) related to 70% dark chocolates of black and brown colors from a previous work of our group.MethodsColor analysis and untargeted high-resolution mass spectrometry-based metabolomic analysis were performed on eight beans of each type. Mass spectral data processing, univariate and multivariate statistical methods were conducted for classification of beans and selection of discriminant features.Results and discussionThe results showed that the color difference already observed for black and brown chocolates preexists in the beans. Black and brown beans had 45 and 50 discriminant features, respectively, of which 16 and 41 were phenolic compounds. Most of them were also previously identified as discriminating compounds for black and brown chocolates. Black beans predominantly contained glycosylated flavanols, ranging from monomers to trimers, with dimers and trimers being A-type procyanidins, along with a phenolic acid (protocatechuic acid), and an O-glycosylated flavonol (quercetin-3-O-glucoside). In contrast, brown beans mostly contained non-glycosylated B-type procyanidins ranging from dimers to decamers, but also dimers and trimers of A-type procyanidins, and a glycosylated and sulfated flavanol ((epi) catechin hexoside-sulfate). These markers may be useful for quality control purposes and may contribute to the selection of beans that yield black or brown dark chocolates.

Research continues to provide compelling insights into potential health benefits associated with diets rich in plant-based natural products (PBNPs). Coupled with evidence from dietary intervention trials, dietary recommendations increasingly include higher intakes of PBNPs. In addition to health benefits, PBNPs can drive flavor and sensory perceptions in foods and beverages. Chardonnay marc (pomace) is a byproduct of winemaking obtained after fruit pressing that has not undergone fermentation. Recent research has revealed that PBNP diversity within Chardonnay marc has potential relevance to human health and desirable sensory attributes in food and beverage products. This review explores the potential of Chardonnay marc as a valuable new PBNP ingredient in the food system by combining health, sensory, and environmental sustainability benefits that serves as a model for development of future ingredients within a sustainable circular bioeconomy. This includes a discussion on the potential role of computational methods, including artificial intelligence (AI), in accelerating research and development required to discover and commercialize this new source of PBNPs.

Hyperactivation of blood platelets, one of the causes of heart attack, and other cardiovascular diseases (CVDs), is influenced by various dietary components, including phenolic compounds from vegetables, fruits, teas, wines, cocoa and its products, including chocolate. The present paper sheds new light on the effect of cocoa and its products, especially dark chocolate, on the number and function of blood platelets, and the anti-platelet activity of their constituent phenolic compounds. A review was performed of papers identified in various electronic databases, including PubMed, Science Direct, Scopus, Web of Knowledge, and Google Scholar, with the aim of determining whether their anti-platelet activity may serve as part of a sweet strategy in countering CVDs. Various studies demonstrate that cocoa consumption, especially in the form of dark chocolate, with a high flavanol concentration, has anti-platelet activity and may play a significant role in cardioprotection; they also note that cocoa consumption may be a good strategy in diminishing cardiovascular risk, including hyperactivation of blood platelets. [ABSTRACT FROM AUTHOR]

'Red Meat Honey Crisp (RMHC)' has been widely cultivated by growers in recent years due to its early maturity, and red meat type characteristics. As a bud variant of 'Super Red (SR)' peach, red flesh is the most distinctive characteristic of 'Red Meat Honey Crisp (RMHC)'. However, the mechanism of red flesh formation in 'RMHC' remains unclear. In this study, 79 differentially produced metabolites were identified by metabolomics analysis. The anthocyanin content in 'RMHC' was significantly higher than that in 'SR' during the same period, such as cyanidin O-syringic acid and cyanidin 3-O-glucoside. Other flavonoids also increased during the formation of red flesh, including flavonols (6-hydroxykaempferol-7-O-glucoside, hyperin), flavanols (protocatechuic acid, (+)-gallocatechin), and flavonoids (chrysoeriol 5-O-hexoside, tricetin). In addition, transcriptomic analysis and RT-qPCR showed that the expression levels of the flavonoid synthesis pathway transcription factor MYB75 and some structural genes, such as PpDFR, PpCHS, PpC4H, and PpLDOX increased significantly in 'RMHC'. Subcellular localization analysis revealed that MYB75 was localized to the nucleus. Yeast single hybridization assays showed that MYB75 bound to the cis-acting element CCGTTG of the PpDFR promoter region. The MYB75-PpDFR regulatory network was identified to be a key pathway in the reddening of 'RMHC' flesh. Moreover, this is the first study to describe the cause for red meat reddening in 'RMHC' compared to 'SR' peaches using transcriptomics, metabolomics and molecular methods. Our study identified a key transcription factor involved in the regulation of the flavonoid synthetic pathway and contributes to peach breeding-related efforts as well as the identification of genes involved in color formation in other species. [ABSTRACT FROM AUTHOR]

The flavanol composition of red wine, stemming from grape skins and seeds, is a determinant factor of its quality. It has been proposed that the wine flavanol structure depends on the grape cultivar but with skin-derived flavanols prevailing in traditional red winemaking from mature grapes. However, this has not been confirmed for many cultivars. This study analysed the flavanol composition in grapes, wines, and pomace of Syrah, Marselan, and Tannat, Vitis vinifera cultivars known for their potential to yield tannic wines. Across two vintages, 20 wines were produced through traditional winemaking. Flavanols were analysed by HPLC–MS/MS before and after acid-catalysed depolymerisation of proanthocyanidins. Grapes of different ripeness were considered as vineyard with different agronomical characteristics. Nevertheless, each cultivar exhibited a characteristic flavanol composition in both grapes and wines. Notably, all Marselan wines had high proportions of seed-derived flavanols (high proportions of monomers, dimers and low of prodelphinidins), from grapes with high skin flavanol content. In contrast, Tannat and Syrah wines had high proportions of skin-derived flavanols, irrespective of the corresponding seed flavanol levels in grapes. Flavanol extractability from skins mainly regulated wine composition, being unusually low in Marselan, rising with ripeness in Syrah, and consistently high in Tannat, masking the high seed flavanol contents of its grapes. Therefore, wines with high proportions of seed-derived flavanols can be obtained from fermentative macerations of ripe grapes. Pomaces were found to be a rich source of flavanols, primarily from seeds where they largely remain unextractable. [ABSTRACT FROM AUTHOR]

This research is the first study of McIntosh apple cider fermentation using different must treatments. The must materials included standard pressed juice, the common cider fermentation material, mash, direct from the apple shredder both with and without pectinase additions, and finally, pomace. These four treatments caused multiple differences from the standard hard ciders from juice, starting with the apple must characteristics, following through the yeast fermentation processes, and carried into the final ciders. Initial apple musts had different sugar content, pH, acids, total phenolics, and antioxidant activities. Although juice contained more total phenolics and had more antioxidant capabilities, it contained lower levels of yeast assimilable nitrogen. The sugar consumption dynamic changes had a differential dynamic trend but did not alter the capacity for complete apple cider fermentation. From the fermentation color dynamic changes, it indicated that must materials would have effects on color-changing amplitudes. Juice treatment had the largest changes from the must. Pomace and mash with pectinase had fewer color changes in multiple color values (L*, a*, b*). The mono phenolics in the final cider indicated that pomace ferments contained the least hydroxycinnamates but a similar amount of total flavanols as juice-fermented ciders. Cider from the juice contained the least flavonols, whereas the mash, both with and without pectinase treatments, had the largest amount of flavonols. This work will provide some applicable information for apple cider fermentation from the apple wastes in the cider industry. [ABSTRACT FROM AUTHOR]

Copyright of Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas is the property of Universidad de Santiago de Chile and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The pathophysiology of muscle damage in peripheral artery disease (PAD) includes increased oxidant production and impaired antioxidant defenses. Epicatechin (EPI), a naturally occurring flavanol, has antioxidant properties that may mediate the beneficial effects of natural products such as cocoa. In a phase II randomized trial, a cocoa-flavanol-rich beverage significantly improved walking performance compared with a placebo in people with PAD. In the present work, the molecular mechanisms underlying the therapeutic effect of cocoa flavanols were investigated by analyzing baseline and follow-up muscle biopsies from participants. Increases in nuclear factor erythroid 2-related factor 2 (Nrf2) target antioxidants heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase [quinone] 1 (NQO1) in the cocoa group were significantly associated with reduced accumulation of central nuclei, a myopathy indicator, in type II muscle fibers (P ¼ 0.017 and P ¼ 0.023, respectively). Protein levels of the mitochondrial respiratory complex III subunit, cytochrome b-c1 complex subunit 2 (UQCRC2), were significantly higher in the cocoa group than in the placebo group (P ¼ 0.032), and increases in UQCRC2 were significantly associated with increased levels of Nrf2 target antioxidants HO-1 and NQO1 (P ¼ 0.001 and P ¼ 0.035, respectively). Exposure of non-PAD human myotubes to ex vivo serum from patients with PAD reduced Nrf2 phosphorylation, an indicator of activation, increased hydrogen peroxide production and oxidative stress, and reduced mitochondrial respiration. Treatment of myotubes with EPI in the presence of serum from patients with PAD increased Nrf2 phosphorylation and protected against PAD serum-induced oxidative stress and mitochondrial dysfunction. Overall, these findings suggest that cocoa flavanols may enhance antioxidant capacity in PAD via Nrf2 activation. NEW & NOTEWORTHY The current study supports the hypothesis that in people with PAD, cocoa flavanols activate Nrf2, thereby increasing antioxidant protein levels, protecting against skeletal muscle damage, and increasing mitochondrial protein abundance. These results suggest that Nrf2 activation may be an important therapeutic target for improving walking performance in people with PAD. [ABSTRACT FROM AUTHOR]

Soil N deficiency is one of the main constraints limiting high tea yields worldwide, particularly in Russia. In addition, high dosages of N fertilizers are widely applied for tea plantations, resulting in agrogenic transformation of soils, environmental pollution, and a decrease in tea quality. One of the ways to reduce dosages of N fertilizers is the development of new cultivars with high efficiency of N use. In this regard, the effect of genotype on biosynthesis and accumulation of valuable secondary metabolites in tea leaves was studied under optimal N supply and its deficiency in a potted experiment. The study was conducted on the most promising local tea cultivars (Kolkhida and Karatum) grown on brown forest acidic soils in the humid subtropics of Russia. Using high-performance liquid chromatography, the contents of catechins, flavanols, alkaloids, and theanine were evaluated in tea leaves. Our results showed that N availability stimulated the biosynthesis of theanine more than one order of magnitude and alkaloids (caffeine by three to five times and theobromine by two to three times) in both tea genotypes; however, it decreased the accumulation of several catechins and flavanols by on average 1.5–2 times. Under optimal N supply, the Kolkhida cultivar displayed higher accumulation of theanine (by 30–60%) and gallated catechins (by 50%) in the leaves; however, higher accumulation of alkaloids (caffeine and theobromine) was observed in the Karatum cultivar (by 10–20%). N deficiency resulted in greater accumulation of simple and gallated catechins, as well as rutin in the Kolkhida cultivar, as compared to the Katarum cultivar. However, the content of the studied metabolites in the Katarum cultivar was more stable under different nitrogen levels, indicating its lower susceptibility to N deficiency. [ABSTRACT FROM AUTHOR]

The viability, productivity and survival of higher plants under the adverse factors influence are largely determined by the functional activity of the antioxidant system. The aim of our work was to investigate changes in formation of high-molecular (superoxide dismutase and peroxidase) and low-molecular (phenolics, including flavanols and proanthocyanidins) antioxidants in callus culture of Camellia sinensis under influence of phenolic precursors (L-phenylalanine—3 mM, trans-cinnamic acid—1 mM, naringenin—0.5 mM). According to the data obtained, the effect of precursors on tea callus cultures did not lead to significant increasing of superoxide dismutase and peroxidase activity in most cases. However, it led to the increased accumulation of the total phenolics content, as well as flavanols and proanthocyanidins contents. For C. sinensis callus cultures, the most promising regulator of phenolic compounds was L-phenylalanine, in the presence of which its content increased almost twice. Thus, the exogenous effect of various precursors is possible to use for the targeted regulation of certain phenolics classes accumulation in plant cells. [ABSTRACT FROM AUTHOR]

The chemical composition of foods is complex, variable, and dependent on many factors. This has a major impact on nutrition research as it foundationally affects our ability to adequately assess the actual intake of nutrients and other compounds. In spite of this, accurate data on nutrient intake are key for investigating the associations and causal relationships between intake, health, and disease risk at the service of developing evidence-based dietary guidance that enables improvements in population health. Here, we exemplify the importance of this challenge by investigating the impact of food content variability on nutrition research using three bioactives as model: flavan-3-ols, (–)-epicatechin, and nitrate. Our results show that common approaches aimed at addressing the high compositional variability of even the same foods impede the accurate assessment of nutrient intake generally. This suggests that the results of many nutrition studies using food composition data are potentially unreliable and carry greater limitations than commonly appreciated, consequently resulting in dietary recommendations with significant limitations and unreliable impact on public health. Thus, current challenges related to nutrient intake assessments need to be addressed and mitigated by the development of improved dietary assessment methods involving the use of nutritional biomarkers.

The objective of the study was to determine the effect of vintage and cultivar on the total polyphenol content and the antioxidant activity of wines made from the white wine cultivar ‘Solaris’ and the red wine cultivar ‘Zilga’ (both with skin maceration). The second goal was to describe the polyphenolic profile of ‘Solaris’ wine and compare it with that of ‘Zilga’ wine. Spectrophotometric methods were employed to determine the total polyphenol content and the antioxidant activity. High-performance liquid chromatography (HPLC) was used to determine the polyphenol composition. The total polyphenol content and the antioxidant activity of ‘Solaris’ wine differed significantly from that of ‘Zilga’ wine in the experimental years from 2021 to 2023. Significant differences between the wines also occurred on average over the years, with a significantly higher total polyphenol content and antioxidant activity obtained for the red wine of ‘Zilga’. However, both wines were similarly influenced by vintage over the three-year experimental period. The antioxidant activity showed a high correlation with the total polyphenol content. The polyphenolic profile of ‘Solaris’ wine differed notably from that of ‘Zilga’ wine. While ‘Solaris’ wine exhibited lower levels of phenolic acids and flavonols, the content of the flavanols was significantly higher, even double that of ‘Zilga’ wine. Among the flavanols, there was a remarkably high content of catechin and epicatechin.

Hyperactivation of blood platelets, one of the causes of heart attack, and other cardiovascular diseases (CVDs), is influenced by various dietary components, including phenolic compounds from vegetables, fruits, teas, wines, cocoa and its products, including chocolate. The present paper sheds new light on the effect of cocoa and its products, especially dark chocolate, on the number and function of blood platelets, and the anti-platelet activity of their constituent phenolic compounds. A review was performed of papers identified in various electronic databases, including PubMed, Science Direct, Scopus, Web of Knowledge, and Google Scholar, with the aim of determining whether their anti-platelet activity may serve as part of a sweet strategy in countering CVDs. Various studies demonstrate that cocoa consumption, especially in the form of dark chocolate, with a high flavanol concentration, has anti-platelet activity and may play a significant role in cardioprotection; they also note that cocoa consumption may be a good strategy in diminishing cardiovascular risk, including hyperactivation of blood platelets.

Defatted seed residues after the extraction of rose oil have their potential not fully described in the existing literature. The aim of this study was to determine and characterize the components important for the human body that are found in Rosa rugosa defatted seeds, including dietary fibers, proteins, selected minerals, polyphenols and antioxidant activity. Rosa rugosa seeds defatted with CO2 in supercritical conditions are a rich source of dietary fibers (approx. 65%) and proteins (15%); their macronutrients include the following: Ca (175.9), Mg (83.9), K (199.2) and Na (3.5 mg/100 g). They also contain polyphenols, including flavanols (0.9%) and total ellagic acid (0.5%), and they exhibit antioxidant activity (143.8 µM TAEC/g). Tellimagrandin I and II and rugosin A were found in the extracts, and ellagitannins with a yet-indeterminate structure were also present. The seeds also contained ellagitannin derivatives—galloyl-HHDP-glucose and bis-HHDP-glucose—at the same time, and they are characterized by a low-fat content—0.4%. The energy value of defatted rose seeds is about half the energy value of popular seeds used in the food industry. The findings of the present study suggest that defatted rosehip seeds, the by-product of rosehip processing, could be an important source of bioactive components like dietary fibers, flavanols, ellagitannins and mineral compounds. Therefore, defatted rose seeds are very promising and require further research, because they can potentially be used as a natural source of chemopreventive agents. [ABSTRACT FROM AUTHOR]

The effect of different intensity of light exposure (5000 and 10 000 lux) on the growth of two strains (1 and 2) of the tea plant (Camellia sinensis L.) callus cultures and the accumulation of flavanols in them, including oligomeric forms—proanthocyanidins. The composition and content of monomeric flavanols—(+)-catechin and (–)-epicatechin in methanol extracts of cultures were studied by high-performance liquid chromatography (HPLC). Data on the morphology of calluses are presented and the greening of the strain 1 is noted, especially at high light intensity (10 000 lux). Its high ability to accumulate flavanols was established, which was 12–35 times higher than that of the strain 2. These differences depended on the age of the cultures and the intensity of light exposure. By HPLC method, monomeric flavanols—(+)-catechin and (–)-epicatechin were identified in methanol extracts of the callus strain 1, the amount of which increased at the end of the passage. Calluses of the low-producing strain 2 are characterized by the formation of only (–)-epicatechin, the amount of which did not change during their growth and did not depend on the intensity of light exposure. All this indicates significant differences in the accumulation of flavanols in the two strains of tea callus cultures and their response to light of different intensities. [ABSTRACT FROM AUTHOR]

This study provides a detailed report on the physicochemical, bioactive components, and volatile profiles of diverse sour cherry (Prunus cerasus) cultivars to identify the cultivar(s) containing high health-promoting components. Physiological characteristics (fruit weight, size dimensions, moisture, color attributes, total soluble solids, pH, titratable acidity, maturity index, nutritional bioactive components (total phenolic, total anthocyanin content, and total flavonoids), antioxidant activity, and volatile profile of 10 sour-cherry cultivars, consisting of dark red Morello type and clear fruit flesh Amarelle type, were studied. The total phenolic content was in the range of 123.24–289.91 mg gallic acid equivalent/100 g FW (fresh weight), total flavonoids (1340.23–2831.91 mg quercetin equivalent/100 g FW), and total anthocyanins (225.43–485.66 mg cyanidin-3-glucoside equivalent (CGE)/100 g FW) in different sour-cherry cultivars, showing significant diversity in such health-promoting compounds. In vitro antioxidant activity assessed by ferric reducing antioxidant potential was observed in the range of 658.18–1483.37 mg Trolox equivalent (TE)/100 g FW and by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) at 384.36 and 931.85 mg TE/100 g FW. A total of 10 phenolic components, including five hydroxycinnamic acids, three flavonoids (flavanols), and one anthocyanin, have been identified and quantified by high-performance liquid chromatography. Hydroxycinnamic acids represented 40%–60% of total phenolic components, while flavonoids and anthocyanins amounted to 20% each in total phenolic composition. The volatile profile of sour-cherry cultivars revealed that aldehydes, alcohols, ketones, esters, monoterpenes, acids, sugars, and hydrocarbons were the predominant volatiles present in sour cherry. [ABSTRACT FROM AUTHOR]

Pecan nuts (Carya illinoinensis) provide a wide range of bioactive compounds (particularly polyphenols) that improve the nutritional quality of diets. This study aimed to monitor the evolution of polyphenolic compounds (total phenols, total flavonoids, total flavanols, total condensed tannins, and total o-diphenols), the phenolic profile, the antioxidant activity, and the sugar concentration during pecan nut development in three Tunisian cultivars. Condensed tannins (41.98–221.13 mg catechin assay equivalents g−1 FW) were the dominant class of phenolics at all maturity stages, followed by total phenols (12.25–57.92 mg gallic acid equivalents g−1 FW). Ellagic acid and catechin were the most abundant phenolics at all maturity stages. The highest phenolic content and antioxidant activity were found at an early stage of ripening but as maturity progressed, a decreasing trend was observed. Sucrose (6.09–30.79 mg g−1 FW) was the predominant sugar followed by fructose and then glucose. A decreasing and later increasing trend of total carbohydrate concentration was detected during nut development. A Canonical Discriminant Analysis of the data succeeded in separating the three pecan cultivars due to their unique characteristics. Overall, the cultivar and the degree of maturity are the major factors controlling the chemical composition and antioxidant capacity of pecan nuts. This study provides more information on the optimal period when the maximum concentration of these health-enhancing compounds is found for use in food, nutraceutical, cosmetic, and pharmaceutical applications. [ABSTRACT FROM AUTHOR]

Background: This study investigated the ultraviolet (UV) absorption spectra of various types and ages of grape wines and the correlation these spectra presented with their phenolic constituents. Firstly, the differences in UV spectra were characterized for different wine samples, depending on their type and age. Methods: The following methods were used in this study: ultraviolet visible spectrophotometry, Folin–Ciocalteu spectrophotometric method, high-performance liquid chromatography. Results: Then, it was demonstrated that for identically aged wines, the 280 nm absorbance is proportional to the concentration of phenolic compounds, as determined by the Folin–Ciocalteu method. Next, an investigation was conducted into the absorption coefficients of different phenolic classes commonly found in grapes and wine. Finally, the range in variation of phenolic compounds in various types of grape wines was established. Conclusions: This work provides a methodological approach to rapidly determine the concentration of phenolic compounds in wines using UV spectroscopy, provided that their age is known. As UV spectrophotometers are available in nearly all laboratories, this may provide a cheaper and faster alternative to current methods, including high-performance liquid chromatography (HPLC).

Inheritance and mutations are important factors affecting grape phenolic composition. To investigate the inter- and intra-varietal differences in polyphenolic compounds among grapes and wines, 27 clones belonging to eight varieties of Vitis vinifera L. were studied over two consecutive years. A total of 24 polyphenols (nine anthocyanins, three flavanols, five flavonols, and seven phenolic acids) were analyzed, and the physicochemical parameters of the grapes and wines were determined. Polyphenol profiles showed significant varietal and clonal polymorphisms, and malvidin-3-O-glucoside, peonidin-3-O- glucoside, and epicatechin were identified as key biomarkers distinguishing different grapes and wines when using an orthogonal partial least squares discriminant analysis. Further multivariate analysis classified these genotypes into three subclasses, and a somatic variant of ‘Malbec’, MBVCR6, had the most abundant polyphenolic compounds that were related to the titratable acid content. The current results reveal that varietal and clonal variations are important for obtaining wines with high polyphenol content.

Excessive galloylated flavanols not only cause instability in the wine but also lead to unbalanced astringency. Although clarification agents are always used to precipitate unstable tannins in wine, the non-specific adsorption of tannins results in the failure to precisely regulate the tannin composition of the wine. In this work, molecularly imprinted polymers (MIPs) with template molecules of galloylated flavanols were designed to specifically adsorb gallotannins to reduce wine astringency. The results showed that the "pores" on the surface of the MIPs are the structural basis for the specific adsorption of the target substances, and the adsorption process is a chemically driven single-molecule layer adsorption. Moreover, in the mono/oligomeric gallotannin-rich model solution, the adsorption of gallotannins by I-MIPs prepared as single template molecules reached 71.0%, and the adsorption capacity of MIPs for monomeric gallotannins was about 6.0 times higher than polymeric gallotannins. Given the lack of technology for the targeted adsorption of tannins from wine, this work explored the targeted modulation of wine astringency by using molecular imprinting techniques. [ABSTRACT FROM AUTHOR]

The objective of this study was to optimize different vacuum drying conditions for cocoa pod husk and cocoa bean shell in order to enhance these by-products for commercial applications. To carry out the optimization, the response surface methodology was applied using a Box–Behnken experimental design with 15 experiments for which different conditions of temperature (X1), drying time (X2) and vacuum pressure (X3) were established. The response variables were the content of total polyphenols, the content of flavanols and the radical scavenging activity evaluated in the extracts of the different experiments. Temperature (50–70 °C), drying time (3–12 h) and vacuum pressure (50–150 mbar) were considered as independent variables. The main factors affecting the response variables were temperature, followed by vacuum pressure. For the content of polyphenols, the optimal response values predicted for the cocoa pod husk was 11.17 mg GAE/g with a confidence limit (95%) of 9.05 to 13.28 mg GAE/g (optimal conditions: 65 °C, 8 h and 75 mbar), while for the cocoa bean shell cocoa was 29.61 mg GAE/g with a confidence limit (95%) of 26.95 to 32.26 mg GAE/g (optimal conditions: 50 °C, 5 h and 100 mbar). Therefore, results of this study suggest a high content of phenolic compounds obtained from these by-products that show relevance as functional ingredients for application in the food, nutraceutical, and cosmeceutical industries. [ABSTRACT FROM AUTHOR]

Anneslea fragrans Wall., popularly known as "Pangpo tea", is an edible, medicinal, and ornamental plant of the Family Theaceae. The leaves of A. fragrans were historically applied for the treatment of liver and intestinal inflammatory diseases in China. This study aimed to explore the hepatoprotective agents from A. fragrans leaves through hepatoprotective and anti-inflammatory assessment. The phytochemical investigation of the leaves of A. fragrans resulted in the isolation and identification of a total of 18 chemical compounds, including triterpenoids, aliphatic alcohol, dihydrochalcones, chalcones, flavanols, phenolic glycoside, and lignans. Compounds 1–2, 4–6, 11–12, and 16–18 were identified from A. fragrans for the first time. Compounds 7 and 14 could significantly alleviate hepatocellular damage by decreasing the contents of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and inhibit the hepatocellular apoptosis in the HepG2 cells induced by N-acetyl-p-aminophenol (APAP). In addition, compounds 7 and 14 inhibited reactive oxygen species (ROS) and malondialdehyde (MDA) contents and increased the catalase (CAT) superoxide dismutase (SOD), and glutathione (GSH) levels for suppressing APAP-induced oxidative stress. Additionally, compounds 7, 13, and 14 also had significant anti-inflammatory effects by inhibiting interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) productions on LPS-induced RAW246.7 cells. [ABSTRACT FROM AUTHOR]

The study aimed to characterize hydro-methanolic (25%) extracts of Justicia adhatoda L. (stem and flower) and Cordia dichotoma Frost. (Stem and fruit) and evaluate the in silico thrombolytic properties of the major phytocompounds present in the plants. In the preliminary fluorescence imaging through treatment by different reagents, both plants were found to be pharmacologically active. Further qualitative screening of phytochemicals, spectroscopy-based techniques, namely, UV-Vis Spectroscopy and FTIR, revealed various classes of compounds such as polyacetylenes, aglycones, quercetin, anthocyanins, anthraquinones, alkaloids, chalcones and aurones, flavanols, carotenoids, and flavanones. Further, by the application of Thin Layer Chromatography, phenols and flavonoids, namely Catechol, Kaempferol, Quercetin, and Lutein, along with other compounds like Chlorophyll b, Glutamic Acid, and Tryptophan were identified from the extracts. Finally, in the molecular docking study, three compounds, Datiscoside and Robinin of C. dichotoma and Daucosterol of J. adhatoda showed high binding energies (-10.224, -9.547 and -9.262 kcal mol-1 respectively) towards the G-protein coupled thrombotic platelet aggregation receptor P2Y1 when compared to that of the control MRS2500 (-7.148 kcal mol-1). [ABSTRACT FROM AUTHOR]

Dietary flavanols are known for disease preventative properties but are often poorly absorbed. Gut microbiome flavanol metabolites are more bioavailable and may exert protective activities. Using metabolite mixtures extracted from the urine of rats supplemented with flavanols and treated with or without antibiotics, we investigated their effects on INS-1 832/13 β-cell glucose stimulated insulin secretion (GSIS) capacity. We measured insulin secretion under non-stimulatory (low) and stimulatory (high) glucose levels, insulin secretion fold induction, and total insulin content. We conducted treatment-level comparisons, individual-level dose responses, and a responder vs. non-responder predictive analysis of metabolite composition. While the first two analyses did not elucidate treatment effects, metabolites from 9 of the 28 animals demonstrated significant dose responses, regardless of treatment. Differentiation of responders vs. non-responder revealed that levels of native flavanols and valerolactones approached significance for predicting enhanced GSIS, regardless of treatment. Although treatment-level patterns were not discernable, we conclude that the high inter-individual variability shows that metabolite bioactivity on GSIS capacity is less related to flavanol supplementation or antibiotic treatment and may be more associated with the unique microbiome or metabolome of each animal. These findings suggest flavanol metabolite activities are individualized and point to the need for personalized nutrition practices. [ABSTRACT FROM AUTHOR]

(1) Background: Cocoa's healthy benefits may be attributed to the potent antioxidant activity of cocoa polyphenols, mainly flavanols, which have been characterised as existing in a high concentration in cocoa. However, the phenolic composition of cocoa and cocoa-derived products is highly variable, and manufacturing processes might significantly reduce their phenolic content. For that reason, the full characterisation of cocoa and cocoa-derived products before evaluating their bioactivity is crucial. The aim of this review is to analyse the available evidence on the effect of flavanol-fortified cocoa-derived products on human health. (2) Methods: Forty-eight clinical trials focused on the health effect of consuming flavanol-fortified drinks, bars and chocolate have been reviewed, with a total of 1523 subjects. (3) Results: Although studies differ widely in methodology, dosage, duration, and target population, beneficial effects of flavanol-rich cocoa consumption have been observed at doses ranging from 45.3 mg/d to 1078 mg/d, especially on cardiovascular health and cognitive function. (4) Conclusions: Considering the high consumption and acceptability of cocoa and cocoa-derived products, the fortification of cocoa products as well as other highly consumed foods with cocoa flavanols could be an effective strategy for health promotion. [ABSTRACT FROM AUTHOR]

The combination of fruit pulps from different species, in addition to multiplying the offer of flavors, aromas and textures, favors the nutritional spectrum and the diversity of bioactive principles. The objective was to evaluate and compare the physicochemical characteristics, bioactive compounds, profile of phenolic compounds and in vitro antioxidant activity of pulps of three species of tropical red fruits (acerola, guava and pitanga) and of the blend produced from the combination. The pulps showed significant values of bioactive compounds, with emphasis on acerola, which had the highest levels in all parameters, except for lycopene, with the highest content in pitanga pulp. Nineteen phenolic compounds were identified, being phenolic acids, flavanols, anthocyanin and stilbene; of these, eighteen were quantified in acerola, nine in guava, twelve in pitanga and fourteen in the blend. The blend combined positive characteristics conferred by the individual pulps, with low pH favorable for conservation, high levels of total soluble solids and sugars, greater diversity of phenolic compounds and antioxidant activity close to that of acerola pulp. Pearson's correlation between antioxidant activity and ascorbic acid content, total phenolic compounds, flavonoids, anthocyanins and carotenoids for the samples were positive, indicating their use as a source of bioactive compounds. [ABSTRACT FROM AUTHOR]

Background: Oral cancer has a high prevalence worldwide, and this disease is caused by genetic, immunological, and environmental factors. The main risk factors associated with oral cancer are smoking and alcohol. Results: There are various strategies to reduce risk factors, including prevention programs as well as the consumption of an adequate diet that includes phytochemical compounds derived from cranberries (Vaccinium macrocarpon A.) and blueberries (Vaccinium corymbosum L.); these compounds exhibit antitumor properties. Results: The main outcome of this review is as follows: the properties of phytochemicals derived from cranberries were evaluated for protection against risk factors associated with oral cancer. Conclusions: The secondary metabolites of cranberries promote biological effects that provide protection against smoking and alcoholism. An alternative for the prevention of oral cancer can be the consumption of these cranberries and blueberries. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effects of consuming 50 g of raisins on cognitive performance, quality of life, and functional activities in healthy older adults. This is a parallel randomized controlled clinical trial, in which 80 subjects over 70 years of age participated. For 6 months, the intervention group (IG; n = 40) consumed 50 g of raisins per day added to their usual diet, whereas the control group (CG; n = 40) received no supplement. All variables were measured at baseline and at 6 months. Cognitive performance assessed with the Montreal Cognitive Assessment (MOCA) test shows a difference of 3.27 points (95% CI 1.59 to 4.96), p ≤ 0.001, favorable to the IG, after the intervention. Among the cognitive performances, an improvement is observed in the IG in orientation, assessed both with the MOCA test 0.49 (95% CI 0.10 to 0.87), p = 0.014, and with the Mini-Mental State Examination (MMSE) test, 0.36 (95% CI 0.02 to 0.70), p = 0.038. In visuospatial/executive capacity and in language, improvements were also observed in the IG, 1.36 (95% CI 0.77 to 1.95), p = 0.001, and 0.54 points (95% CI 0.12 to 0.96), p = 0.014, respectively. Immediate and delayed recall, assessed with the Rey Auditory Verbal Learning Test, improved in the IG. In addition, the IG showed a better quality of life and greater autonomy in instrumental activities of daily living after 6 months. No significant changes were observed in the rest of the variables analyzed. Therefore, the consumption of 50 g of raisins produces a slight improvement in cognitive performance, quality of life, and functional activities in the elderly. [ABSTRACT FROM AUTHOR]

Dietary flavanols are food constituents found in certain fruits and vegetables that have been linked to cognitive aging. Previous studies suggested that consumption of dietary flavanols might specifically be associated with the hippocampal-dependent memory component of cognitive aging and that memory benefits of a flavanol intervention might depend on habitual diet quality. Here, we tested these hypotheses in the context of a large-scale study of 3,562 older adults, who were randomly assigned to a 3-y intervention of cocoa extract (500 mg of cocoa flavanols per day) or a placebo [(COcoa Supplement and Multivitamin Outcomes Study) COSMOS-Web, NCT04582617]. Using the alternative Healthy Eating Index in all participants and a urine-based biomarker of flavanol intake in a subset of participants [n = 1,361], we show that habitual flavanol consumption and diet quality at baseline are positively and selectively correlated with hippocampal-dependent memory. While the prespecified primary end point testing for an intervention-related improvement in memory in all participants after 1 y was not statistically significant, the flavanol intervention restored memory among participants in lower tertiles of habitual diet quality or habitual flavanol consumption. Increases in the flavanol biomarker over the course of the trial were associated with improving memory. Collectively, our results allow dietary flavanols to be considered in the context of a depletion-repletion paradigm and suggest that low flavanol consumption can act as a driver of the hippocampal-dependent component of cognitive aging. [ABSTRACT FROM AUTHOR]

Summary: Grape juice is a major source of potential health‐promoting bioactive polyphenols, especially for children and those who do not consume wine. Since the subtropical climate may negatively affect the concentrations of grape polyphenols, especially anthocyanins, elicitors such as methyl jasmonate (MeJa) could be used to promote polyphenol biosynthesis. This work aimed at investigating the impact of MeJa treatment on grape juice produced via a traditional low‐cost process from two Vitis labrusca cultivars and in two Brazilian regions. The untargeted LC–MS analytical protocol demonstrated that Isabel Precoce juices strongly benefited from MeJa treatment, especially regarding their anthocyanic profile, regardless of the cultivation region. Known MeJa markers in wine and V. vinifera grapes (flavanols, flavonols and stilbenes) in this experiment had mixed behaviours depending on the region/variety/cultivation. Moreover, it was found that all the detected hydroxycinnamates were influenced by the treatment, especially the concentration of their glucosides, which was increased. Glutathione, 2‐S‐glutathionyl caftaric acid and indole lactic acid glucoside were identified for the first time as MeJa treatment biomarkers in grape products, indicating a possible positive effect on juice antioxidant properties. [ABSTRACT FROM AUTHOR]

Recent studies on natural antioxidant compounds have highlighted their potentiality against various pathological conditions. The present review aims to selectively evaluate the benefits of catechins and their polymeric structure on metabolic syndrome, a common disorder characterized by a cluster of three main risk factors: obesity, hypertension, and hyperglycemia. Patients with metabolic syndrome suffer chronic low inflammation state and oxidative stress both conditions effectively countered by flavanols and their polymers. The mechanism behind the activity of these molecules has been highlighted and correlated with the characteristic features present on their basic flavonoidic skelethon, as well as the efficient doses needed to perform their activity in both in vitro and in vivo studies. The amount of evidence provided in this review offers a starting point for flavanol dietary supplementation as a potential strategy to counteract several metabolic targets associated with metabolic syndrome and suggests a key role of albumin as flavanol-delivery system to the different target of action inside the organism. [ABSTRACT FROM AUTHOR]

The aim of the present study was to analyze the effects of cocoa flavanols and red berry anthocyanins on cardiovascular biomarkers, such as homocysteine, angiotensin-converting enzyme (ACE), nitric oxide (NO), flow-mediated vasodilation (FMD), blood pressure and lipid profile. Additionally, we aimed to ascertain their possible interactions with microbiota related metabolites, such as secondary bile acids (SBA), short-chain fatty acids (SCFA) and trimethylamine N-oxide (TMAO). A randomized, parallel-group study, single-blind for the research team, was performed on 60 healthy volunteers between the ages of 45 and 85, who consumed 2.5 g/day of cocoa powder (9.59 mg/day of total flavanols), 5 g/day of a red berry mixture (13.9 mg/day of total anthocyanins) or 7.5 g/day of a combination of both for 12 weeks. The group that had consumed cocoa showed a significant reduction in TMAO (p = 0.03) and uric acid (p = 0.01) levels in serum, accompanied by an increase in FMD values (p = 0.03) and total polyphenols. corrected by creatinine (p = 0.03) after the intervention. These latter values negatively correlated with the TMAO concentration (R = −0.57, p = 0.02). Additionally, we observed an increase in carbohydrate fermentation in the groups that had consumed cocoa (p = 0.04) and red berries (p = 0.04) between the beginning and the end of the intervention. This increase in carbohydrate fermentation was correlated with lower levels of TC/HDL ratio (p = 0.01), systolic (p = 0.01) and diastolic blood pressure (p = 0.01). In conclusion, our study showed a positive modulation of microbiota metabolism after a regular intake of cocoa flavanols and red berry anthocyanins that led to an improvement in cardiovascular function, especially in the group that consumed cocoa. [ABSTRACT FROM AUTHOR]

Walnuts are one of the healthiest foods in the world because they are one of the best sources of beneficial nutrients, minerals, and antioxidants. They also contain key sources of energy. Despite several traditional uses, the leaves of Juglans regia L. have received little attention regarding phytochemical and pharmacological potential. Thus, the current study intended to find the walnut genotypes with the greatest antioxidant, anticancer, and antifungal activity. The total polyphenolic, flavonoid, and flavanol contents of leaves from 14 walnut genotypes were determined. Genotypes that accumulate flavonoid/flavonol contents (99.8–111.93 mg/g quercetin equivalent (QE) and 101.67–111.83 mg/g QE) showed significantly higher ferric reducing antioxidant potential (FRAP) activity (128.2–148.1 μM Fe2+/g dry weight (DW)] than other genotypes. Maximum divergence in the quercetin content (0.8–1.23 mg/g) of walnut genotypes was obtained by cluster analysis. The active component, quercetin, was measured using RP-HPLC. Moreover, the extracts were investigated for antifungal and anticancer assays. We report the significant antifungal potential of walnut leaf genotypes against Candida glabrata, Candida albicans, and Candida tropicalis, with 57.7–93.6%, 26.8–51.5%, and 26.8–51.5% inhibition, respectively. The most significant antiproliferative effect was shown by Opex Culchry, which exhibited 9.4% cell viability at a concentration of 25 µL (0.75 mg) against lung (A549) cell lines. Chenovo exhibited 2.9, 6.2, and 2.2% cell viability, Opex Culchry exhibited 2, 1.5, and 2.4% cell viability, and Sulieman showed 7.6, 0.9, and 7% cell viability against the colon (HCT116) cell lines. The results showed that walnut leaves possess enormous potential as antioxidants, and as anticancer and antifungal agents. [ABSTRACT FROM AUTHOR]

A new 1,2-seco-flavan-3-ol,(2S)-1-(3′′-methoxy-4′′-hydroxyphenyl)-3-(2′,6′-dimethoxy-4′-hydroxyphenyl)-propan-2-ol (1), together with nine known compounds (2–10) were isolated from the leaves of Cinnamomum camphora var. linaloolifera Y. Fujita. Their structures were identified by spectroscopic methods and comparison with reported references. Compounds 1, 2, and 7 have different degrees of inhibition on HepG2 cells, IC50 values vary between 50 and 110 μmol·L–1. [ABSTRACT FROM AUTHOR]