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10. Chemical profile and toxicogenetic safety assessment of Smallanthus sonchifolius (yacon) organic extracts.

Author

Moreira Szokalo, Rocío Ayelén, Ulloa, Jerónimo, Tulino, María Soledad, Redko, Flavia, Flor, Sabrina, Muschietti, Liliana, and Carballo, Marta A.

Subjects
YACON, SESQUITERPENE lactones, ACYCLIC acids, CYTOTOXINS, EXTRACTS
Abstract

The interest in Smallanthus sonchifolius (yacon) has strongly resurfaced due to its multiple beneficial effects on human health. This study aimed at determining the toxicity and the chemical profile of an ethanol extract (EE) and a crude lactone mixture (CLM) of yacon leaves. Cytotoxicity and genotoxicity tests were performed by the MTT assay and the alkaline version of the comet assay respectively. The phytochemical analysis, performed by chromatographic and spectroscopy techniques, revealed the presence of nine sesquiterpene lactones (STLs) and two acyclic diterpene acids. In all cases, cell viability was inversely proportional to the extract concentration employed. The effects obtained with the highest dose of EE were significantly different from those obtained with the negative and solvent controls. Conversely, no significant differences were observed between the lowest doses of EE and controls. As for CLM, all tested doses showed statistically significant increases, as compared to negative and solvent controls. [ABSTRACT FROM AUTHOR]

Published
2023
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18. In vitro anti-inflammatory properties of Smilax campestris aqueous extract in human macrophages, and characterization of its flavonoid profile

Author

Salaverry, Luciana S., primary, Parrado, Andrea C., additional, Mangone, Franco M., additional, Dobrecky, Cecilia B., additional, Flor, Sabrina A., additional, Lombardo, Tomás, additional, Sotelo, Agustina D., additional, Saccodossi, Natalia, additional, Rugna, Ana Z., additional, Blanco, Guillermo, additional, Canellada, Andrea, additional, and Rey-Roldán, Estela B., additional

Published
2020
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21. A capillary electrophoretic system based on a novel microemulsion for the analysis of coenzyme Q10 in human plasma by electrokinetic chromatography

Author

Silvia, Lucangioli, Sabrina, Flor, Flor, Sabrina, Mario, Contin, Contin, Mario, Valeria, Tripodi, and Tripodi, Valeria

Subjects
Adult, Male, Accuracy and precision, Ubiquinone, Capillary action, Butanols, Clinical Biochemistry, Analytical chemistry, Sensitivity and Specificity, Biochemistry, Analytical Chemistry, Bile Acids and Salts, Surface-Active Agents, chemistry.chemical_compound, Humans, Microemulsion, Chromatography, High Pressure Liquid, Chromatography, Micellar Electrokinetic Capillary, Octane, Chromatography, Butanol, Temperature, Electrophoresis, Capillary, Reproducibility of Results, Succinates, Hydrogen-Ion Concentration, Octanes, Hexane, Electrophoresis, chemistry, Linear Models, Emulsions, Female, Steroids, Selectivity
Abstract

A new analytical method for determination of coenzyme Q10 (2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone, CoQ10) in human plasma was developed based on CE using a double tensioactive microemulsion. CoQ10 was quantitatively extracted into 1-propanol/hexane and quantified by MEEKC. The microemulsion was prepared by mixing 1.4% w/w sodium bis(2-ethylhexyl) sulfosuccinate, 4% w/w cholic acid, 1% w/w octane, 8.5% w/w butanol, 0.1% w/w PVA and 85% w/w 10 mM Tris buffer at pH 9.0. The optimized electrophoretic conditions included the use of an uncoated silica capillary of 60 cm total length and 75 mum id, an applied voltage of 20 kV, room temperature and 214 nm ultraviolet detection. Selectivity, linearity, LOD, LOQ, precision and accuracy were evaluated as the parameters of validation. Owing to its simplicity and reliability, the proposed method can be an advantageous alternative to the traditional methodology for the quantitation of CoQ10 in human plasma with good accuracy and precision.

Published
2009
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24. Electrophoretic deposition of gentamicin-loaded bioactive glass/ 2 chitosan composite coatings for orthopaedic implants

Author

Pishbin, Fatemehsadat, Mouriño, Viviana Silvia Lourdes, Flor, Sabrina Andrea, Kreppel, Stefan, Salih, Vehid, Ryan, Mary P., and Boccaccini, Aldo R.

Subjects
Chitosan, Recubrimientos y Películas, purl.org/becyt/ford/2 [https], Coatings, Ingeniería de los Materiales, Bioactive Glass, Electrophoretic Deposition, INGENIERÍAS Y TECNOLOGÍAS, Drug Delivery, purl.org/becyt/ford/2.5 [https], Gentamicin
Abstract

Despite their widespread application, metallic orthopaedic prosthesis failure still occurs because of lack of adequate bone-bonding and the incidence of post-surgery infections. The goal of this research was to develop multifunctional composite chitosan/Bioglass coatings loaded with gentamicin antibiotic as a suitable strategy to improve the surface properties of metallic implants. Electrophoretic deposition (EPD) was applied as a single-step technology to simultaneously deposit the biopolymer, bioactive glass particles, and the antibiotic on stainless steel substrate. The microstructure and composition of the coatings were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively. The in vitro bioactivity of the coatings was demonstrated by formation of hydroxyapatite after immersion in simulated body fluid (SBF) in a short period of 2 days. High-performance liquid chromatography (HPLC) measurements indicated the release of 40% of the loaded gentamicin in phosphate buffered saline (PBS) within the first 5 days. The developed composite coating supported attachment and proliferation of MG-63 cells up to 10 days. Moreover, disc diffusion test showed improved bactericidal effect of gentamicin-loaded composite coatings against S. aureus compared to control non-gentamicin-loaded coatings. Fil: Pishbin, Fatemehsadat. Imperial College London; Reino Unido Fil: Mouriño, Viviana Silvia Lourdes. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kreppel, Stefan. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania Fil: Salih, Vehid. University College London; Estados Unidos Fil: Ryan, Mary P.. Imperial College London; Reino Unido Fil: Boccaccini, Aldo R.. Imperial College London; Reino Unido. Universitat Erlangen-Nuremberg; Alemania

Published
2014
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26. Characterization of a microemulsion system with AOT as pseudostationary phase in MEEKC for the analysis of estrogens

Author

Flor, Sabrina Andrea, Tripodi, Valeria Paula, Estevez, Pablo, and Lucangioli, Silvia Edith

Subjects
Otras Ciencias de la Salud, CIENCIAS MÉDICAS Y DE LA SALUD, PSEUOSTATIONARY PHASE, ESTROGENS, Ciencias de la Salud, AOT, MICROEMULSION
Abstract

Microemulsion electrokinetic chromatography (MEEKC) is an electrodriven separation technique, which employs a microemulsion (ME) as pseudostationary phase (PSP) in capillary electrophoresis. In recent years, MEEKC has become an important tool for achieving the separation of a diverse range of solutes. Microemulsions are composed of nanometer-sized oil droplets suspended in aqueous buffer, which is commonly referred to as oil-in-water ME. The aim of this work was the characterization of a ME based on sodium bis(2-ethylhexyl)sulfosuccinate (AOT) as pseudostationary phase, developed for the simultaneous determination of natural and synthetic estrogens by MEEKC. The ME system consisted of 1.4% w/w AOT,1.0% w/w octane, 7.0% w/w 1-butanol and 90.6% w/w 20 mM sodium salt of 3(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO) and 10 mM phosphate buffer at pH 12.5. The AOT MEEKC system was characterized electrophoretically, physicochemically and morphologically and parameters such as microdroplets phase residence times (tmic) and microdroplets proportion (tprop,mic) were calculated for the first time in order to study the interaction between the solutes and the PSP. In order to characterize the AOT microemulsion regions, a phase diagram was constructed, and two regions, a W/O and another O/W were determined. Dynamic viscosity was determined and dynamic light scattering and transmission electron microscopy were performed to complete the characterization of the PSP. Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Estevez, Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Published
2013

27. Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC

Author

Flor, Sabrina Andrea, Tripodi, Valeria Paula, Chiappetta, Diego Andrés, Iglesias Rando, Matías, and Lucangioli, Silvia Edith

Subjects
HYDROPHOBICITY, Ingeniería Química, PSEUDOSTATIONARY PHASE, Ingeniería de Procesos Químicos, RETENTION, Tecnología de Laboratorios Médicos, INGENIERÍAS Y TECNOLOGÍAS, POLYMERS, MIXED-MICELLES, Ingeniería Médica
Abstract

The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system. Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina; Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina; Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Published
2013

43. New analytical strategies applied to the determination of Coenzyme Q10 in biological matrix.

Author

Contin M, Flor S, Martinefski M, Lucangioli S, and Tripodi V

Subjects
Blood Platelets metabolism, Emulsions, Humans, Muscles metabolism, Phase Transition, Reference Standards, Ubiquinone blood, Ubiquinone chemistry, Chromatography, High Pressure Liquid methods, Electrophoresis, Capillary methods, Ubiquinone analogs & derivatives
Abstract

In the last few years the importance of Coenzyme Q10 (CoQ10) determination has gained clinical relevance. CoQ10 is a redox-active, lipophilic substance integrated in the mitochondrial respiratory chain which acts as an electron carrier for the production of cellular energy. In addition, it is recognized as a primary regenerating antioxidant playing an intrinsic role against oxidative damage. There are some reports of low CoQ10 levels in a number of disorders, such as cancer, muscular, neurodegenerative, cardiological, and reproductive diseases. Therefore, it is a priority to develop analytical methodologies for evaluating CoQ10 in matrices of greater importance for the correct diagnosis of diseases, simple enough to be used in routine clinical laboratories. In this chapter two recently developed techniques, capillary electrophoresis and microHPLC, for the analysis of CoQ10 in biological matrices, are studied.

Published
2015
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44. A capillary electrophoretic system based on a novel microemulsion for the analysis of coenzyme Q10 in human plasma by electrokinetic chromatography.

Author

Lucangioli S, Flor S, Contin M, and Tripodi V

Subjects
Adult, Bile Acids and Salts chemistry, Butanols chemistry, Chromatography, High Pressure Liquid methods, Female, Humans, Hydrogen-Ion Concentration, Linear Models, Male, Octanes chemistry, Reproducibility of Results, Sensitivity and Specificity, Steroids chemistry, Surface-Active Agents chemistry, Temperature, Ubiquinone blood, Ubiquinone chemistry, Chromatography, Micellar Electrokinetic Capillary methods, Electrophoresis, Capillary methods, Emulsions chemistry, Succinates chemistry, Ubiquinone analogs & derivatives
Abstract

A new analytical method for determination of coenzyme Q10 (2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone, CoQ10) in human plasma was developed based on CE using a double tensioactive microemulsion. CoQ10 was quantitatively extracted into 1-propanol/hexane and quantified by MEEKC. The microemulsion was prepared by mixing 1.4% w/w sodium bis(2-ethylhexyl) sulfosuccinate, 4% w/w cholic acid, 1% w/w octane, 8.5% w/w butanol, 0.1% w/w PVA and 85% w/w 10 mM Tris buffer at pH 9.0. The optimized electrophoretic conditions included the use of an uncoated silica capillary of 60 cm total length and 75 mum id, an applied voltage of 20 kV, room temperature and 214 nm ultraviolet detection. Selectivity, linearity, LOD, LOQ, precision and accuracy were evaluated as the parameters of validation. Owing to its simplicity and reliability, the proposed method can be an advantageous alternative to the traditional methodology for the quantitation of CoQ10 in human plasma with good accuracy and precision.

Published
2009
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