1. Screening of Natural Products Inhibitors of SARS-CoV-2 Entry.
- Author
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González-Maldonado, Pamela, Alvarenga, Nelson, Burgos-Edwards, Alberto, Flores-Giubi, Eugenia, Barúa, Javier E., Romero-Rodríguez, Cristina, Soto-Rifo, Ricardo, Valiente-Echeverría, Fernando, Langjahr, Patricia, Cantero-González, Guadalupe, and Sotelo, Pablo H.
- Abstract
The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. The entry of the virus into the cell is one of the main targets for inhibiting SARS-CoV-2 infection. Natural products are an important source of new therapeutic alternatives against diseases. Pseudotyped viruses allow the study of SARS-CoV-2 viral entry inhibitors, and due to their simplicity, they allow the screening of a large number of antiviral candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 with the D614G SARS-CoV-2 spike glycoprotein to test its ability to infect ACE2-expressing HEK 293T cells in the presence of diverse natural products, including 21 plant extracts, 7 essential oils, and 13 compounds from plants and fungi. The 50% cytotoxic concentration (CC
50 ) was evaluated using the resazurin method. From these analyses, we determined the inhibitory activity of the extract of Stachytarpheta cayennensis, which had a half-maximal inhibitory concentration (IC50 ) of 91.65 µg/mL, a CC50 of 693.5 µg/mL, and a selectivity index (SI) of 7.57, indicating its potential use as an inhibitor of SARS-CoV-2 entry. Moreover, our work indicates the usefulness of the pseudotyped-virus system in the screening of SARS-CoV-2 entry inhibitors. [ABSTRACT FROM AUTHOR]- Published
- 2022
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