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Desde sus inicios, la medicina nuclear se ha enfrentado a cambios tecnológicos que la han obligado a modificar sus modos operativos y a adecuar sus protocolos. En el campo de la cirugía radioguiada (CRG), la incorporación de la imagen gammagráfica preoperatoria y la detección intraoperatoria con la sonda gamma proporcionó un impulso definitivo a la biopsia del ganglio centinela (GC) para convertirse en el procedimiento estándar de aplicación en el melanoma y el cáncer de mama. Las diversas innovaciones tecnológicas y la adaptación consiguiente de protocolos confluyen en lo disruptivo y lo gradual. Como ejemplos evidentes tenemos la introducción de la tomografía por emisión de fotón único/tomografía computarizada (SPECT/TC) en el campo preoperatorio y las sondas Drop-in (Lightpoint Medical Ltd; Crystal photonics, Eurorad) en el intraoperatorio. Otros aspectos innovadores con posible aplicación en la CRG se basan en la utilización de la inteligencia artificial (IA), navegación y teleasistencia. Since its origins, nuclear medicine has faced technological changes that led to modifying operating modes and adapting protocols. In the field of radioguided surgery, the incorporation of preoperative scintigraphic imaging and intraoperative detection with the gamma probe provided a definitive boost to sentinel lymph node biopsy to become a standard procedure for melanoma and breast cancer. The various technological innovations and consequent adaptation of protocols come together in the coexistence of the disruptive and the gradual. As obvious examples we have the introduction of SPECT/CT in the preoperative field and Drop-in probes in the intraoperative field. Other innovative aspects with possible application in radio-guided surgery are based on the application of artificial intelligence, navigation and telecare. [ABSTRACT FROM AUTHOR]

Introducción. El nitrógeno (N) es un elemento esencial para el proceso de fotosíntesis, y por tanto, determinante en el rendimiento. El uso de sensores ópticos para determinar índices de transmitancia (SPAD, CCI), reflectancia (NDVI) y fluorescencia (Fv.Fm-1) relacionados con el estado del N foliar, permite un adecuado manejo a partir del monitoreo “in situ” no destructivo. Objetivo. Evaluar el efecto de diferentes estrategias de manejo del fertiriego sobre las concentraciones de nitrógeno y clorofila en hoja; y la relación entre i- concentración de N y de Clorofila (Cl) en hoja, ii- concentración de N y de Cl en hoja con el rendimiento y iii- concentración de N y de Cl en hoja con índices SPAD, CCI, NDVI y Fv.Fm-1. Materiales y métodos. Se evaluaron 6 tratamientos en un diseño irrestricto al azar con arreglo factorial 2 x 3, que combinaron 2 estrategias de riego (E1: bajo volumen y de mayor frecuencia y E2: alto volumen y menor frecuencia) y 3 niveles de nutrición: bajo (NB), medio (NM) y alto (NA). Las medidas directas de N y Cl en hoja se determinaron con el método Kjeldahl y por extracción con etanol, respectivamente. Resultados. El nivel de nutrición afectó el contenido de N, donde los tratamientos NM y NA fueron similares, aunque superiores a NB. El modelo de regresión mostró poca capacidad para estimar N y rendimiento a partir del contenido de Cl en hoja. La estimación de Cl en hoja a partir de los índices CCI (R2: 0,71) y SPAD (R2: 0,75) resultaron prometedores solamente en NB. Para estimar N foliar, la regresión con Fv.Fm-1 mostró valores de R2 de 0,89 en NB y 0,69 en NM; mientras que para NDVI los valores R2 fueron 0,61 en NB y 0,47 en NM. Conclusión. Los índices SPAD, CCI, NDVI y Fv.Fm-1 presentaron potencial para estimar la concentración de clorofila y de N en hoja con aportes bajo y medio de nutrientes, y con limitaciones en los tratamientos con altos aportes de nutrición.

Research regarding the conservation of species at risk of extinction is needed worldwide. In this context, the cultivation of seedlings for conservation purposes plays a pivotal role in meeting the demand of projects and the transplantation of these species into natural habitats. There is currently a lack of information regarding the optimal nursery inputs for cultivating high-quality seedlings of Astronium balansae, a forest species native to southern Brazil, Argentina and Paraguay. Therefore, this study aimed to investigate the effect of different substrates and fertilizer sources on the production of A. balansae seedlings in nurseries. At 130 days after sowing, the morphophysiological attributes of the seedlings were evaluated, revealing a significant influence of the chemical and physical characteristics of substrates on plant quality. Controlled-release fertilizer, particularly when used in specific proportions with carbonized rice husk, proved effective in enhancing production. The species exhibits tolerance to low fertility levels and high-porosity substrates; however, under such conditions, it did not exhibit optimal physiological and morphological features. The use of various fertilizers and different doses of carbonized rice husk impacted the quality and production time of A. balansae plants. Morphological and physiological characteristics demonstrated that the application of controlled-release fertilizer at 6 g L-1, in association with 0 %, 20 %, and 40 % carbonized rice husk, enhanced plant quality within 130 days. All the evaluated attributes can be utilized to assess the quality of A. balansae plants. [ABSTRACT FROM AUTHOR]

A strategy that could partially or totally reduce the use of chemical synthesis fertilizers is the application of cyanobacteria with agricultural potential that promote plant growth, which powers them in the conception of a sustainable agriculture. The study evaluated the effect of four concentrations of an extract of Limnospira maxima (0, 4, 8, and 12 mg mL-1), on some physiological aspects of Stevia rebaudiana and Solanum melongena in house vegetation. Treatments were arranged under a complete random design and parameters of gas exchange, chlorophyll a fluorescence, biometric characteristics and biomass distribution were measured. The most important results indicated that in Stevia rebaudiana and Solanum melongena the rates of net photosynthesis increased respectively by 40,03% and 22,45% when cyanobacteria was applied. On the other hand, plants of both species that were inoculated required less electrons to maximize their photosynthesis rates, increased leaf area, plant height and biomass distribution in roots and leaves. The most important physiological indicator was net photosynthesis and this was positively correlated with almost all the parameters studied. The results suggest that the use extracts of L. maxima may be a biotechnological alternative of organic fertilization useful for the development of sustainable agriculture. [ABSTRACT FROM AUTHOR]

Copyright of Universitas Scientiarum is the property of Pontificia Universidad Javeriana and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Global coral bleaching reflects coral fragility to adverse environmental conditions and climate change. Porites lobata is the most abundant hermatypic coral species currently in El Salvador and has experienced recent bleaching events at natural protected area Complejo Los Cóbanos. Objective: To describe the bleaching episodes that colonies of P. lobata experienced at Los Cóbanos, El Salvador, during the last 18 years, and analyze coral restoration efforts. Methods: Approximately 2 100 images (2006 - 2022) taken without specific periodicity from two sites were reviewed. Water parameters since 2014 were registered without defined frequency. The areas sampled were El Faro beach and subtidal colonies near an important beach resort. The first area presented dispersed colonies on an intertidal zone, the other, larger colonies at 2 m deep in low tide. Also, literature about activities to restore reefs within the country was reviewed. Results: Several bleaching events occurred during this observation period. Some of them could be associated to high sea water temperatures (> 30 o C) from May to October (rainy season), others to harmful algae blooms, as well as unknown causes. Severe bleaching that caused loss of coral coverage on intertidal colonies occurred during the global event (2015-2016). Conclusion: Bleaching at Los Cóbanos occurs in different intensity patterns usually during the rainy season that encompasses high temperature and turbidity levels in water, as well as algal blooms. The 2015-2016 episode caused coral coverage loss on the intertidal colonies and subtidal ones experienced little permanent damages (< 5% mortality). National restoration policies for coral colonies require more strategic efforts considering the local littoral hydrodynamics and institutional capabilities. [ABSTRACT FROM AUTHOR]

La investigación busca determinar la influencia de la incorporación de vidrio pulverizado y conchas de abanico calcinadas en la resistencia a la comprensión del concreto f’c=210 kg/cm2. La metodología empleada en la investigación es de tipo aplicada, el diseño es experimental con un enfoque cuantitativo y las muestras están integradas por una cantidad de 18 testigos. Los resultados obtenidos nos indican que: a los 7 días la fuerza es de 194.5kg/cm2 en el concreto patrón y de 196.5kg/cm2 en el concreto muestra; en el día 14 fue de 237.8kg/cm2 en el concreto patrón y de 238.9kg/cm2 en el concreto muestra. Por último, a los 28 días se observó que la resistencia a la compresión en el concreto patrón fue de 295.4kg/cm2 menor que el concreto muestra que fue de 306.1kg/cm2, resaltando que al emplear vidrio pulverizado y conchas de abanico calcinadas mejora la calidad del concreto de diseño f¨c=210 kg/cm2.

Copyright of Revista Mexicana de Ciencias Agrícolas is the property of Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Indocyanine green is a dye that has been used in medicine for several decades. It has a number of applications, including reconstructive surgery and burns. It allows the detection of areas of tissue with reduced perfusion, which reduces the risk of postoperative complications in the form of altered healing processes and necrosis. The imaging technique that uses this dye allows us to observe the changes in fluorescence in real time that have been shown to occur between the superficial and deep layers in burns. This allows a qualitative and quantitative diagnosis of the depth of the burn, which results in the choice of additional treatment. The particular importance of this method in the prevention of skin necrosis with the areolanipple complex is appreciate during simultaneous breast reconstruction. More prospective randomized controlled trials are needed to consider it the 'method of choice' in clinical practice. [ABSTRACT FROM AUTHOR]

El abordaje quirúrgico de las distintas enfermedades, no solo oncológicas, ha evolucionado. Como ha sido acuñado de forma muy gráfica por el grupo de Veronesi, estamos pasando de los «tratamientos máximos tolerables a los mínimos eficaces» y este recorrido no puede ser llevado a cabo de otra forma que mediante un planteamiento de trabajo multidisciplinar y multimodalidad. Obligatoriamente multidisciplinar porque es necesaria la colaboración entre cirujanos, oncólogos, radiólogos, médicos nucleares, patólogos y todo aquel relacionado con el seguimiento de los pacientes, y obligatoriamente multimodalidad porque nos debemos encaminar hacia una cirugía de precisión a la medida de cada paciente en la que, por parte de la Medicina Nuclear, tiene mucho que decir la imagen híbrida (SPECT/TC y PET/TC), los trazadores bimodales, el empleo de nuevos aliados como la ecografía o nuestra propia adaptación a la cirugía robótica. Todo un amplio abanico de posibilidades construido a partir de un sólido cimiento como es la gammagrafía preoperatoria, que posibilita la identificación de los tejidos diana y cuyo conocimiento previo a la intervención permite plantear el abordaje quirúrgico necesario para cada paciente. The surgical approach to different pathologies, not only oncological, has evolved. As Veronesi's group has coined very graphically, we are moving from "maximum tolerable treatments to minimum effective treatments" and this journey cannot be carried out in any other way than through a multidisciplinary and multimodality approach. Multidisciplinary, because collaboration between surgeons, oncologists, radiologists, nuclear physicians, pathologists, and all those involved in patient follow-up is necessary, and multimodality, because we must move towards precision surgery tailored to each patient in which, on the part of Nuclear Medicine, hybrid imaging (SPECT/CT and PET/CT), bimodal tracers, the use of new allies such as ultrasound or our own adaptation to robotic surgery have a great deal to say. A wide range of possibilities is built on the solid foundation of preoperative scintigraphy, which makes it possible to identify the target tissues and whose knowledge prior to surgery allows the necessary surgical approach to be considered for each patient. [ABSTRACT FROM AUTHOR]

There is great interest in studying the optical properties of organic materials because of their fast optical response and excellent integrability in devices, which makes them attractive for applications in Photonics and organic electronics. Solvatochromism allows the analysis of the molecular microenvironment and provides information on the nature of the states responsible for the optical transitions of these materials. In this context, a solvatochromic study was performed on three molecules derived from triphenylamine bound to three electron acceptor groups; Cyanobenzene, Cyanopyridine and Dicyanobenzene, where their absorption and fluorescence spectra were analyzed; the difference of the maximum peaks was correlated with the Onsager polarity function from whose slope, the difference of the permanent dipole moment of each derivative was estimated. For all three molecules, a positive solvatochromism was observed, revealing that the permanent dipole moment of the first excited state is greater than that of the fundamental. As for the results of these differences, they are moderately high, suggesting that there is high electronic mobility throughout the organic compound, leading to the inference that they will have a greater effect on nonlinear optical properties. [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the application effects of fungicides based on strobilurins and carboxamides on the physiology, management of Alternaria sp. and production of tomato fruits in a protected environment. Two experiments were carried out in Salto-SP in 2018 and 2019. The randomized block design was used, with seven treatments: inoculated control (application of water and inoculum with Altenaria sp.), absolute control (application of water), azoxystrobin, boscalid, boscalid + azoxystrobin, pyraclostrobin and boscalid + pyraclostrobin, and five replications. To verify the photosynthetic efficiency of plants, analysis of chlorophyll a fluorescence and leaf gas exchange was performed at 50, 95 and 120 DAT. It was also verified the disease severity (%) and finally and the total and commercial production of fruits. From the results, under this research conditions, the tested fungicide application promoted positive physiological responses in tomato plants, controlling early blight disease and increasing fruit production. The application of the fungicides boscalid and azoxystrobin + boscalid can be a management tool for Alternaria sp. for tomatoes, for controlling the progress of the disease as well as for promoting better physiological and productive responses. [ABSTRACT FROM AUTHOR]

Copyright of Archives of Dental Science / Arquivos em Odontologia is the property of Universidade Federal de Minas Gerais, Faculdade de Odontologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

En el presente trabajo se analiza la distribución de concentración de clorofila a en la región de la plataforma patagónica austral entre los 47° S-54° S a partir de mediciones in situ realizadas durante marzo-abril de 2000. Las observaciones de campo se realizaron con muestreadores discretos (botellas Niskin) y continuos (fluorómetros). Se identificaron dos áreas con altos valores de clorofila (» 1,6 mg m-3) en la zona central de plataforma correspondiente al área entre las isobatas de 100 m y 150 m y en un núcleo secundario en la zona costera de Bahía Grande al sur de la desembocadura del Río Santa Cruz. Los valores mínimos se asociaron a la franja costera próxima a la isobata de 50 m y a la región sur de la plataforma patagónica donde se registraron valores inferiores a 0,5 mg m-3. La relación fluorescencia : concentración de clorofila a fue más variable en las muestras de superficie que a distintas profundidades de la columna de agua. Se analizan las posibles causas de la distribución vertical de clorofila a.

En el año 2006 se identificó el primer movimiento transfronterizo de peces cebra fluorescentes al territorio peruano. Se tiene poco conocimiento del posible impacto ambiental ante la liberación descontrolada de estos peces transgénicos fluorescentes. Así como, los orígenes y flujo génico de estos organismos hidrobiológicos. Se analizó por PCR los genes de fluorescencia GFP (Proteína fluorescente verde) y RFP (Proteína fluorescente roja) en peces cebra transgénicos introducidos dentro del territorio peruano y de peces cebra procedentes de Colombia, Chile y de Taiwán como controles positivos. Todas las secuencias nucleotídicas correspondían a la proteína fluorescente roja (drFP583) de la anémona marina Discosoma sp. con un tamaño de 505pb (RFP) y de su variante verde (GFP) con un tamaño de 570pb. Asimismo, el análisis filogenético evidenció un origen asiático del movimiento transfronterizo de estos peces por una ruta sudamericana vía Colombia principalmente hacia territorio peruano.

Copyright of Revista Brasileira de Reprodução Animal is the property of Revista Brasileira de Reproducao Animal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Regeneration of the dura mater following duraplasty using a collagen film, a chitosan film, or a combination of both with gelatin, was studied in a craniotomy and penetrating brain injury model in rats. Collagen autofluorescence in the regenerated dura mater was evaluated using confocal microscopy with excitation at lem = 488 nm and lem = 543 nm. An increase in regeneration of the extracellular matrix of connective tissue and an increase in matrix fluorescence were detected at 6 weeks after duraplasty. The major contributors to dura mater regeneration were collagen films, chitosan plus gelatin-based films, and, to a much lesser extent, chitosan-based films. By using autofluorescence densitometry of extracellular matrix, the authors were able to quantify the degree of connective tissue regeneration in the dura mater following duraplasty. [ABSTRACT FROM AUTHOR]

A nivel hidrobiológico, el territorio peruano cuenta año tras año con más especies ornamentales de diferentes colores fluorescentes por oleadas de movimientos transfronterizos a pesar de una Ley de moratoria vigente. Desde la obtención de los primeros peces ornamentales fluorescentes transgénicos a principios del siglo XXI, el número de especies hidrobiológicas se ha incrementado. Así como, la producción de nuevos “colores fluorescentes” obtenidos por la inserción de transgenes de especies de invertebrados marinos dentro de peces dulceacuícolas. A partir de su descubrimiento en el año 2006 en el Perú, se ha realizado la identificación de su fluorescencia artificial por luz UV o actínica. Asimismo, se ha efectuado la identificación a nivel molecular de los diversos transgenes que determinan el color corporal de estos peces diseñados por ingeniería genética. Hoy a casi una década de culminar la Ley de la moratoria se especula sobre su posible origen (USA y asiático) y posible flujo génico una vez que son reproducidos dentro del territorio peruano y comercializados a otros países sudamericanos. De esta manera, la presente revisión busca reunir y cruzar toda la información pertinente existente a la fecha, analizarla y dar una proyección futura de estos peces ornamentales transgénicos después del año 2021 cuando la Ley de la moratoria finalice.

A utilização e consumo pouco frequente de leguminosas em Portugal, principalmente pelos mais jovens, e a reduzida disponibilidade de opções de produtos saudáveis com leguminosas conduziram ao desenvolvimento deste projeto: materiais, formas e interações numa experiência alimentar com leguminosas e pressupostos sociais, éticos e estéticos, despertando sobretudo a visão e o gosto. Através de um questionário dirigido ao público em geral, foram analisados os conhecimentos e o consumo de leguminosas, tendo sido realizada uma pesquisa visual e gráfica do comércio e conteúdo online com base num processo criativo em design, usando técnicas de geração de ideias. Neste contexto, o produto desenvolvido é um churro de cacau, com toppings, produzidos à base de leguminosas, que fluorescem sob luz negra, um snack doce, nutricionalmente equilibrado e bastante apelativo para os jovens, estimulando o consumo de leguminosas.

Copyright of Revista Brasileira de Engenharia Agricola e Ambiental - Agriambi is the property of Revista Brasileira de Engenharia Agricola e Ambiental and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The objective of this experiment was to study the effects of different concentrations of the plant growth regulators prohexadione-calcium (ProCa) on the growth control of tomato seedlings. The experiment was conducted in the forest seedling nursery of the Faculty of Agronomic Sciences - UNESP, Botucatu Campus-SP. The experimental design was completely randomized, composed of five treatments: 0, 50, 100, 200 and 400 mg of the active ingredient (a.i.) of ProCa, with 4 replicates of 30 seedlings. Treatments were applied with a manual CO2 sprayer when seedlings completed 20 days after sowing. Ten days after applying the treatments, seedling growth analysis, SPAD index, gas exchange, chlorophyll a fluorescence and biochemical analyses were all performed. From the results obtained, the conclusion was that ProCa concentrations inhibited seedling growth without compromising the photosynthetic apparatus. [ABSTRACT FROM AUTHOR]

Copyright of Ciência Florestal (01039954) is the property of Ciencia Florestal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of RGO: Revista Gaúcha de Odontologia is the property of RGO: Revista Gaucha de Odontologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Final del formulario Anastomotic dehiscence, a feared complication in colorectal surgery, motivates the search for effective strategies to mitigate its risk. This meta-analysis encompasses all published randomized trials investigating and comparing the impact of indocyanine green (ICG) angiography on this complication. With four studies and 1,109 patients, the intraoperative ICG angiography group demonstrated a significant reduction in the overall rate of anastomotic dehiscence compared to the non-angiography group (7.3% vs. 11.5%; OR: 0.6; 95% CI: 0.4-0.9; p = 0.03). These differences were maintained in rectal surgery subgroup, with no prolongation of surgical time or increase in morbidity and mortality. There were no differences in the left colon surgery group. The evidence provided by this meta-analysis would support the effectiveness of ICG angiography in reducing the incidence of anastomotic leakage in colorectal surgery, advocating for its integration into routine surgical practice., (Copyright © 2024 Asociación Española de Cirujanos. Published by Elsevier España, S.L.U. All rights reserved.)

Introdução: A qualidade microbiológica da água tratada para hemodiálise está diretamente relacionada à ocorrência de infecções e de reações pirogênicas nos pacientes. Objetivo: Determinar o tempo de incubação mínimo e avaliar o desempenho do método microbiológico alternativo para a contagem de bactérias heterotróficas em água de hemodiálise por meio da técnica de detecção microbiana por fluorescência. Método: As análises foram conduzidas com níveis de concentração entre 2,5 x 10-1 e 1,0 x 102 UFC/placa para Pseudomonas aeruginosa, Burkholderia cepacia, Escherichia coli e Staphylococcus aureus. Os testes foram realizados simultaneamente pelos métodos alternativo e tradicional, utilizando o meio de cultura R2A e temperatura de incubação de 24,0°C ± 4,0°C. Os tempos de incubação empregados foram os de 40 h e 120 h, respectivamente. Quatorze amostras de água para hemodiálise foram analisadas para avaliação da equivalência entre os métodos avaliados. Resultados: Os resultados demonstraram que o método alternativo permite a quantificação de bactérias heterotróficas após 40 h de incubação, com precisão, exatidão, especificidade e linearidade para a faixa de 5 a 100 UFC/placa. O limite de detecção do método alternativo é 1 UFC/placa. Conclusões: O método alternativo possui resultados equivalentes ao método tradicional, uma vez que o intervalo de confiança do método alternativo obtido esteve compreendido inteiramente dentro da faixa de equivalência. Portanto, a técnica de detecção microbiana por fluorescência mostrou ser uma opção viável para a implementação de um método microbiológico rápido para a contagem de bactérias heterotróficas em amostras de água tratada para hemodiálise.

La melina es una especie forestal con una alta calidad maderable y de importancia económica para diferentes países tropicales como Colombia. No obstante, existe poca información acerca de su genética, lo cual es pertinente en programas de mejoramiento. Con el fin de determinar el tamaño del genoma de Gmelina arborea (valor C), se obtuvieron suspensiones nucleares a partir de hojas, las cuales se analizaron mediante citometría de flujo. Para la obtención de suspensiones acuosas de núcleos, se evaluaron cuatro buffers de extracción, de los cuales, el buffer LB01 produjo poblaciones nucleares intactas y con mayor homogeneidad. Se analizaron tres especies vegetales como posibles estándares para la determinación del valor C; utilizando tomate como estándar referente, se obtuvo un valor C para melina de 467 Mpb. Este es el primer reporte relacionado con la estimación del valor C en melina, por lo que se genera así información importante para incrementar el conocimiento relacionado con esta especie.

Resumo Neste trabalho, apresentamos um estudo sobre a proposta de luz negra, usando a lanterna de um smartphone em conjunto com um filtro azul, e este conjunto emitiria luz ultravioleta, para realizar experimentos de fluorescência. Os trabalhos publicados com essa proposta não fizeram a análise do espectro de emissão dessa fonte de luz. Utilizando um espectrofotômetro, investigamos a emissão espectral da lanterna de três smartphones diferentes. Também utilizamos alguns filtros para analisar as fontes de luz citadas anteriormente. Realizamos alguns experimentos de fluorescência com uma lanterna de um smartphone e uma lâmpada de luz negra, e comparamos os resultados obtidos.

CdSe and CdSe/ZnS (core/shell) quantum dots with oleic acid as stabilizing agent in organic medium were prepared and their optical properties were examined. For CdSe synthesis, the influence of O2 in the growth kinetics of quantum dots was determined. In the first 90 s, the nanocrystals growth was 1.6 higher in presence of O2 than when reaction was carried out in N2 atmosphere. However, the growth rate with O2 is not favorable because the nanocrystal optical properties were affected: wider absorption band and lower fluorescence that those obtained in inert atmosphere. Properties of CdSe nanocrystals synthesized in inert atmosphere were intensified with 10% of monolayer. For a core with 2.5 nm diameter, the fluorescence quantum yield (ΦFl) in the green region increased from 5.5% to 42.3%. In this work, a low Zn2+ (diethylzinc) precursor concentration was used to produce a. The synthesis process of CdSe / ZnS nanocrystals developed with low concentration of Zn2+ and an excess of S2- can be used to obtain materials with excellent photoluminescent properties for applications such as biomarkers, sensors, catalysis, and solar cells.

Aim: Anastomotic leakage (AL) following colorectal surgery is one of the most devastating complication. The use of indocyanine green fluorescence angiography has been developed as a promising tool to reduce the incidence of AL. The aim of this study is to evaluate the impact of this technique on the prevention of AL. Materials and Method: A prospective study was carried out, including 59 patients undergoing resective colorectal surgery. It was performed intraoperatively indocyanine green angiography evaluation of the anastomotic perfusión in all of then. Results: The section point was modified in 9 patients (15.25%); in which no AL was registered. The overall complication rate was 35.59% (n = 21), founding 3 anastomotic dehiscences in the serie. Conclusion: In conclusion, in our experience this technique is an additional strategy in the prevention of the AL. Randomized control trial including more patients will be necessary to obtain conclusive results. [ABSTRACT FROM AUTHOR]

The indigenous microflora of fish, particularly the microbial ecology of the digestive tract, has been investigated by many researchers. This is due to its assumed importance in digestion and disease control. It is known, mainly from studies of the intestinal microflora of warm blooded animals, that the bacterial population structure of the intestine influences the establishment of pathogenic micro-organisms in the intestinal tract. [ABSTRACT FROM AUTHOR]

This work presents a study about the proposal of the black light using a flashlight of a smartphone with a blue filter, and this set could emitted ultraviolet light, to do experiments of fluorescence. The published works with this porposal did not analyse the emission spectra of light source. Using a spectrophotometer we investigated the emission spectra of the flashlight of three different smartphones. We also used some filters to analyse the light sources cited before. We did experiments of fluorescence with the flashligth of a smartphone and a lamp of dark light, and we compared the obtained results. [ABSTRACT FROM AUTHOR]

Melina, also known as white teak, is a species of high timber quality and economic importance for different tropical countries like Colombia. However, information about its genetics is scarce, which is relevant for breeding programs. In order to determine the genome size of Gmelina arborea (C value), nuclei suspensions were obtained from leaves, and analyzed using flow cytometry. To obtain aqueous nuclei suspensions, four extraction buffers were evaluated, LB01 buffer produced intact populations with greater homogeneity. Three species were analyzed as possible reference standards for the determination of the C value; using tomato as a reference species, a C value for melina of 467 Mpb was obtained. This is the first report related to the estimation of the C value in Gmelina arborea, generating important information to increase knowledge related to this species. [ABSTRACT FROM AUTHOR]

In 2006, the first transboundary movement of fluorescent zebrafish to the Peruvian territory was identified. Little is known about the possible environmental impact due to the uncontrolled release of these transgenic fluorescent fish. As well as, the origins and gene flow of these hydrobiological organisms. The fluorescence genes GFP (Green Fluorescent Protein) and RFP (Red Fluorescent Protein) in transgenic Zebrafish introduced into the Peruvian territory and Zebrafish from Colombia, Chile and Taiwan as positive controls were analyzed by PCR. All nucleotide sequences corresponded to the red fluorescent protein (drFP583) of the marine anemone Discosoma sp. with a size of 505pb (RFP) and its green variant (GFP) with a size of 570pb. Likewise, the phylogenetic analysis evidenced an Asian origin of the transboundary movement of these fish along a South American route via Colombia, mainly to Peruvian territory. [ABSTRACT FROM AUTHOR]

Introdução: Em virtude do interesse para o tratamento da hepatite, o processo de produção industrial do INF-α foi desenvolvido e aperfeiçoado ao longo dos últimos anos. Objetivo: O presente trabalho teve como objetivo desenvolver um protocolo para caracterizar a estrutura molecular do INF-α2b em formulações farmacêuticas por espectrometria de massa do tipo MALDI-TOF. Método: Inicialmente foi desenvolvido um método de cromatografia líquida baseado em fase reversa para promover a separação entre o INF-α2b constituinte ativo e minoritário e a soro albumina humana, também componente presente nas formulações farmacêuticas, obtendo-se amostras com homogeneidade proteica, revelada por eletroforese. As amostras em solução foram submetidas à digestão com tripsina, levadas ao espectrômetro de massa MALDI-TOF. Para que fosse analisada a estrutura molecular, foi desenvolvido um procedimento baseado em imunoafinidade e cromatografia de gel filtração. Resultados: As amostras preparadas por estes métodos apresentaram homogeneidade proteica por eletroforese, sendo analisadas por dicroísmo circular e fluorescência, o que demonstrou ter degradação da estrutura tridimensional. Conclusões: Esse trabalho fornece dados importantes que subsidiam o estabelecimento de um protocolo para a análise de INF-α2b em produto final, que poderia substituir o mapa de peptídeos tradicional por cromatografia líquida, com a vantagem de resultar em um maior número de informações sobre a estrutura molecular do biofármaco. ========================================== Introduction: Due to the interest in the treatment of hepatitis, the industrial production process of INF-α has been developed and perfected over the last few years. Objective: The present work aimed to develop a protocol to characterize the molecular structure of INF-α2b in pharmaceutical formulations by MALDI-TOF mass spectrometry. Method: Initially, a reversed-phase liquid chromatography method was developed to promote the separation of active and minor constituent INF-α2b and human serum albumin, also present in the pharmaceutical formulations, to obtain samples with protein homogeneity revealed by electrophoresis. Samples were hydrolyzed with trypsin and submitted to MALDI-TOF. In order to analyze the molecular structure, a procedure based on immunoaffinity and gel filtration chromatography was developed. Results: Prepared samples by these methods showed protein homogeneity by SDS-PAGE, and were analyzed by circular dichroism and fluorescence, which showed three – dimensional structure degradation. Conclusions: This work provides important data that support the establishment of a protocol for the analysis of INF-α2b in final product, which could replace the traditional peptide mapping by liquid chromatography, with the advantage of resulting in a larger amount of information about the structure of the biopharmaceutical.

Actualmente las herramientas biotecnológicas como la ingeniería genética y la biología molecular se encuentran muy avanzadas, permitiendo el desarrollo de sistemas que se basan en el uso de células vegetales para la producción de proteínas recombinantes, lo cual permite el desarrollo de plataformas para la elaboración de biofármacos, enzimas y metabolitos secundarios. El uso de metodologías para transformación transitoria y estable, permiten la producción de proteínas recombinantes de interés. En este trabajo se realizó una transformación estable de callo de N. tabacum de la línea comercial BY-2 y una silvestre (T1). Además se realizaron ensayos de agroinfiltración por la técnica al vacío y con la técnica por jeringa. En ambos casos, la transformación fue mediada por Agrobacterium tumefaciens LBA4404 la cual poseía el vector pCAMBIA 1303, conteniendo los genes marcadores GFP y GUS, y un gen de resistencia a Higromicina. Se evaluó la expresión de la proteína verde fluorescente por medio de microscopía de fluorescencia. La expresión de los genes de resistencia a Higromicina y β-glucoronidasa se evaluaron por medio de PCR convencional. Se obtuvieron porcentajes de transformación aceptables, en conjunto con una alta expresión de la proteína verde fluorescente.

Since its origins, nuclear medicine has faced technological changes that led to modifying operating modes and adapting protocols. In the field of radioguided surgery, the incorporation of preoperative scintigraphic imaging and intraoperative detection with the gamma probe provided a definitive boost to sentinel lymph node biopsy to become a standard procedure for melanoma and breast cancer. The various technological innovations and consequent adaptation of protocols come together in the coexistence of the disruptive and the gradual. As obvious examples we have the introduction of SPECT/CT in the preoperative field and Drop-in probes in the intraoperative field. Other innovative aspects with possible application in radio-guided surgery are based on the application of artificial intelligence, navigation and telecare., (Copyright © 2023 Sociedad Española de Medicina Nuclear e Imagen Molecular. Published by Elsevier España, S.L.U. All rights reserved.)

At the hydrobiological level, the Peruvian territory has year more year with more ornamental species of different fluorescent colors by waves of transboundary movements despite a current moratorium law. From obtaining the first transgenic fluorescent ornamental fish at the beginning of the 21st century. The number of hydrobiological species has increased year. As well as, the production of new "fluorescent colors" obtained by the insertion of transgenes of marine invertebrate species within freshwater fish. Since its discovery in 2006 in Peru. The identification of its artificial fluorescence by UV or actinic light has been carried out. Likewise, the identification at the molecular level of the various transgenes that determine the body color of these genetically engineered fish has been carried out. Today, almost a decade after the expiration of the moratorium law, there is speculation about its possible origin (USA and Asia) and possible gene flow once they are reproduced within the Peruvian territory and marketed to other South American countries. In this way, this review seeks to gather and cross all relevant information to date, analyze it and give a future projection of these transgenic ornamental fish after the year 2021 when the Law of the moratorium ends. [ABSTRACT FROM AUTHOR]

Oral squamous papilloma is a benign tumor whose pathogenesis has been associated with human papillomavirus infection. Thus, it is noteworthy that human papillomavirus infection is one of the risk factors associated with the development of cervical, anogenital, pharynx, larynx and oral cavity carcinomas. Oral squamous papilloma can affect any region of the oral cavity, and transmission of human papillomavirus can occur by direct contact, sexual intercourse or from mother to child during delivery. The diagnosis is clinical and histopathological, with surgical removal representing the treatment of choice. Recently, widefield optical fluorescence has been used as a complementary examination to the conventional clinical examination in the screening of oral pathological lesions and for the delimitation of surgical margins. We report a case of oral squamous papilloma with its clinical, histopathological features and, in addition, from the perspective of wide field optical fluorescence. [ABSTRACT FROM AUTHOR]

The use of medicinal plants by the Brazilian population is a traditional practice, and is often the only resource used in basic health care. Within the context of the new instrumental analytical techniques, X-ray fluorescence spectroscopy stands out, being used mainly for areas where the rapid profile of constituents is indispensable. The medicinal plants used in this study were: Brazilian arnica (Solidago microglossa D. C.), chamomile (Chamomilla recutita (L.) Rauschert), Chilean boldo (Peumus boldus Molina), lemongrass (Cymbopogon citratus) and Brazilian ginseng (Pfaffia Paniculata (Martius) Kuntze). This work aims to qualitatively analyze, through X ray fluorescence, the elemental composition of selected medicinal plants that are commonly marketed in the state of Rio de Janeiro. By means of the generated energy spectra, Ca, K, Fe, Zn, Ni, among others, were observed. The Chilean boldo, camomila and arnica samples presented a large number of photon counts for Ca and K, while the lemongrass showed a high number of photons for Fe and Ni. However, one of Chilean boldo samples was highlighted as worrying, due to peak in the spectrum, referring to energy that corresponds to a heavy metal, lead (Pb). [ABSTRACT FROM AUTHOR]

A rapid and simple ecotoxicological bioassay allows a reliable estimation of state of the lotic ecosystems from Camana, Majes and Colca watershed located in region Arequipa (Peru) in six sampling points (Taparza, Grande, Majes1, Majes2, Camana1 and Camana2) by studying growth inhibition of the microalga Pseudokirchneriella subcapitata at 24, 48 and 72 hours and Mean Effective Concentration (EC50), at 72 hours compared to National Environmental Quality Standards (EQSs) and water quality guidelines of the World Health Organization (WHO). Observing that in the sampling points of Majes1 and Majes2 surpassed the values of thermotolerant coliforms, Aluminum, Manganese, Iron and Total Suspended Solids (TSS), compared to the values of EQSs and water quality guidelines of the WHO, with an EC50 in the Sampling stations of Majes1 and Majes2 categorizing them as moderately toxic. In this article, fluorescence confocal microscopy techniques were used to evaluate the impact of metals that exceeded the EQSs and WHO, proposing a model as the application of these microscopic techniques, opening wide perspectives, for future studies of metal ecotoxicity. [ABSTRACT FROM AUTHOR]

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Introducción: El microscopio de campo claro es una técnica accesible a la mayoría de laboratorios biológicos, así como su adaptación a la fluorescencia. Debido a una disminución en la profundidad de campo, se encuentran presentes varios planos de focalización en los cuales se puede confirmar que la muestra posee un volumen apreciable. Materiales y métodos: Haciendo uso del microscopio de campo claro, junto a sustancias fluorescentes que preservan las características naturales del espécimen, se hace posible focalizar la muestra en diferentes partes de la misma, a una distancia medible dada por el enfoque fino (0.1 mm por rotación). Resultados y discusión: Los puntos que son enfocados se relacionan con el desplazamiento del objeto (o del objetivo), adquiriendo planos focalizados y localizados espacialmente, generando superficies de alto contraste. Conclusiones: Se reconocen las dimensiones de la muestra; combinándolo con la optimización, se obtienen datos cualitativos de la misma y el mejoramiento en las imágenes obtenidas por la microscopia de campo claro.

O objetivo deste estudo foi avaliar o crescimento, a biomassa seca e indicadores de estresse em mudas de C. guianensis e D. odorata submetidas a estresse hídrico. As plantas foram submetidas a tratamentos com e sem fornecimento de água. Foram coletados altura e diâmetro, biomassa, área foliar, área foliar específica, índice de conteúdo de clorofila e fluorescência da clorofila a. As plantas exibiram aumentos nos valores biométricos e de biomassa em ambos os tratamentos aplicados. O índice de conteúdo de clorofila aumentou em C. guianensis, já em D. odorata manteve constante no tratamento de deficiência hídrica. Nos valores relacionados à fluorescência, verificou-se redução até nas plantas sem deficiência hídrica. Maiores investigações precisam ser realizadas para esclarecer esse comportamento.