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The olfactory response of insect vectors such as phlebotomine sand flies is a key facet for investigating their interactions with vertebrate hosts and associated vector-borne pathogens. Such studies are mainly performed by assessing the electrophysiological response and the olfactory behaviour of these arthropods towards volatile organic compounds (VOCs) produced by hosts. Nonetheless, few studies are available for species of the subgenera Lutzomyia and Nyssomyia in South America, leaving a void for Old World sand fly species of the genus Phlebotomus. In this study, we evaluated the olfactory responses of Phlebotomus perniciosus, one of the most important vectors of Leishmania infantum in the Old World. To test the P. perniciosus behavioural response to VOCs, 28 compounds isolated from humans and dogs were assessed using electrophysiological (i.e., electroantennogram, EAG) and behavioural assays (i.e., Y-tube olfactometer). In the EAG trials, 14 compounds (i.e., acetic acid, nonanoic acid, 2-propanol, 2-butanol, pentanal, hexanal, nonanal, trans-2-nonenal, decanal, myrcene, p-cymene, verbenone, 2-ethyl-1-hexanol, and acetonitrile) elicited high antennal responses (i.e., ≥ 0.30 mV) in female sand flies, being those VOCs selected for the behavioural assays. From the 14 compounds tested in the Y-tube olfactometer, nonanal was significantly attractive for P. perniciosus females, whereas myrcene and p-cymene were significantly repellents (p < 0.05). The attraction indexes varied from 0.53 for nonanal (i.e., most attractive) to -0.47 to p-cymene (i.e., most repellent). Overall, our results shed light on the role of olfactory cues routing host seeking behaviour in P. perniciosus, with implications to develop sustainable sand fly monitoring as well as control in leishmaniasis endemic areas. Author summary: The way insects respond to host odours is a key aspect for understanding how they interact with their hosts and associated pathogens. Such studies are mainly performed by assessing the attraction or repellence of these arthropods towards host emitted volatile organic compounds (VOCs). In this study, we investigated how Phlebotomus perniciosus reacts to host odours. For this, 28 compounds isolated from humans and dogs were assessed by antennal stimulus and odour choice by sand flies. Fourteen compounds elicited high antennal responses in female sand flies, being those VOCs selected for the behavioural assays. From the 14 compounds tested in the Y-tube olfactometer, nonanal was significantly attractive for P. perniciosus females, whereas myrcene and p-cymene acted as repellents (p < 0.05). Data herein obtained highlight the role of olfactory cues routing host seeking behaviour in P. perniciosus, with implications to develop sustainable sand fly monitoring and control, in leishmaniasis endemic areas. [ABSTRACT FROM AUTHOR]

Introduction: Humans are able to regulate the intensity with which they exert cognitive control in interference tasks in terms of factors such as the control level required on the previous trial, and the overall frequency of conflict. However, recent research has shown that the ability to follow explicit cues predicting the required level of control is more limited than previously assumed. Specifically, participants in color Stroop tasks did only take advantage of pre-cues informing them about the congruency of the following trial when the cue was presented in the interval between successive trials, but not when the information was conveyed by the preceding trial. Method: Here we explore the boundary conditions of these sequential cueing effects by using a Stroop task in which the proportion of high-conflict trials was increased, to improve practice with the rules, or decreased, to make the task less demanding. Results: The results showed no effect of trial-by-trial cueing, neither increasing nor decreasing the proportion of high-conflict trials. Furthermore, the cueing effect was not observed either when the cue was conveyed by neutral trials, thus reducing the conflation between the conflict present on a trial and the conflict that this trial predicts. Discussion: As a whole, the results illustrate how difficult it is to adjust control parameters on the fly on the basis of sequential cues, even if they are explicit. [ABSTRACT FROM AUTHOR]

This paper presents a novel modeling approach for flying capacitor dynamics in boost-type multi-level converters (FCML-boosts) controlled by Phase Shift Pulse Width Modulation (PSPWM). By explicitly taking into account the interaction between the inductor current and the flying capacitor voltage, the model is able to reveal an underlying resonance phenomenon and to predict its frequency at each operating point. Based on such a model, whose derivation is explained in detail, both passive and active damping solutions are proposed, designed, and experimentally verified that significantly reduce the undesirable oscillations. The analytical results and the devised control solutions are tested on a 1 k W , four-level, boost DC-DC converter prototype employing 200 V , 48 A rated EPC2034C GaN devices. [ABSTRACT FROM AUTHOR]

Tsetse flies are the sole cyclic vectors of African trypanosomes, which cause human and animal African trypanosomiases in Africa. Tsetse fly control remains a promising option for disease management. The sterile insect technique (SIT) stands as an environmentally friendly tool to control tsetse populations. SIT requires the mass‐rearing of competent sterile males to mate with wild females. However, long‐term colonization might affect the genetic structure of the reared flies. This study investigated the genetic structure of four Glossina palpalis gambiensis colonies of different ages: two originating from Senegal (SEN and ICIRSEN) and two from Burkina Faso (CIR and IBD). Samples from these colonies were genotyped at ten microsatellite loci, followed by downstream population genetic analyses. The results show that the two colonies from Burkina Faso collected from close sites (∼20 km apart) over 45‐year interval retained the same genetic background (FST_CIR∼IBD ≈ 0, P‐value = 0.47). These flies were however, genetically different from those from the Senegal colonies (FST_CIR∼SEN ≈ 0.047; FST_IBD∼SEN ≈ 0.058, P‐value = 10−4). Moreover, no significant difference was detected in the gene diversity of the CIR and IBD colonies, with HS values of 0.650 and 0.665, respectively. The inbreeding coefficient showed that all four colonies where under Hardy–Weinberg equilibrium, with FIS values of 0.026, 0.012, −0.064, and 0.001, for CIR, IBD, ICIRSEN, and SEN, respectively. Furthermore, no sign of a recent bottleneck was identified in tsetse samples from any of the four colonies. The results suggest that long‐term mass‐rearing of tsetse flies has no significant impact on their genetic background and diversity. [ABSTRACT FROM AUTHOR]

Background: Development of insecticide resistance (IR) in sand fly populations is an issue of public health concern, threatening leishmaniasis mitigation efforts by insecticide-based vector control. There is a major knowledge gap in the IR status of wild populations worldwide, possibly attributed to the unavailability of specialized tools, such as bioassay protocols, species baseline susceptibility to insecticides and molecular markers, to monitor such phenomena in sand flies. Methodology/Principal findings: Sand fly populations from (semi-)rural regions of Greece, Turkey and Iran were sampled and identified to species, showing populations' structure in accordance with previously reported data. Genotyping of known pyrethroid resistance-associated loci revealed the occurrence of voltage-gated sodium channel (vgsc) mutations in all surveyed countries. Knock-down resistance (kdr) mutation L1014F was prevalent in Turkish regions and L1014F and L1014S were recorded for the first time in Iran, and in Turkey and Greece, respectively, yet in low frequencies. Moreover, CDC bottle bioassays against pyrethroids in mixed species populations from Greece indicated full susceptibility, using though the mosquito discriminating doses. In parallel, we established a novel individual bioassay protocol and applied it comparatively among distinct Phlebotomus species' populations, to detect any possible divergent species-specific response to insecticides. Indeed, a significantly different knock-down rate between P. simici and P. perfiliewi was observed upon exposure to deltamethrin. Conclusions/Significance: IR in sand flies is increasingly reported in leishmaniasis endemic regions, highlighting the necessity to generate additional monitoring tools, that could be implemented in relevant eco-epidemiological settings, in the context of IR management. Our molecular and phenotypic data add to the IR map in an area with otherwise limited data coverage. Author summary: Phlebotomine sand flies transmit leishmaniasis to humans and animals, a neglected tropical disease of the (sub-)tropics, currently expanding though in previously non-endemic regions. As leishmaniasis eradication largely relies on vectors' insecticidal control, regular monitoring of insecticide resistance (IR) is a core element of integrated vector management. IR data are limited in sand fly populations worldwide though, due to the unavailability of robust molecular diagnostic and phenotyping tools, as well as deficiencies in human resources, appropriate training and capacity building. Here, we aim to characterise the pyrethroid resistance status of populations originating from countries of the Mediterranean basin and the Middle East; i.e. Greece, Turkey and Iran. Pyrethroid resistance-associated mutations were detected in all three countries, while the populations from Greece exhibited susceptibility upon exposure to deltamethrin in CDC bioassays. We established an individual bioassay protocol to enable IR monitoring in settings with multi-species population structure, and applied it comparatively among three distinct Phlebotomus species, revealing that they respond differently to insecticide treatment. Our study contributes data for sustainable evidence-based sand fly control, and highlights the importance of systematic IR surveillance programs in leishmaniasis endemic regions. [ABSTRACT FROM AUTHOR]

Forward models are mechanisms enabling an agent to predict the sensory outcomes of its actions. They can be implemented through efference copies: copies of motor signals inhibiting the expected sensory stimulation, literally canceling the perceptual outcome of the predicted action. In insects, efference copies are known to modulate optic flow detection for flight control in flies. Here we investigate whether forward models account for the detection of optic flow in walking ants, and how the latter is integrated for locomotion control. We mounted Cataglyphis velox ants in a virtual reality setup and manipulated the relationship between the ants' movements and the optic flow perceived. Our results show that ants compute predictions of the optic flow expected according to their own movements. However, the prediction is not solely based on efference copies, but involves proprioceptive feedbacks and is fine-tuned by the panorama's visual structure. Mismatches between prediction and perception are computed for each eye, and error signals are integrated to adjust locomotion through the modulation of internal oscillators. Our work reveals that insects' forward models are non-trivial and compute predictions based on multimodal information. Vertebrates' brains produce predictions of the visual outcome expected from their own movements. Here the authors show that ants' brains also produce such predictions by combining multiple sources of information such as copies of their motor commands, proprioception and the environment's visual structure. [ABSTRACT FROM AUTHOR]

Exaggerated ornaments provide opportunities to understand how selection can operate at different levels to shape the evolution of a trait. While these features aid their bearer in attracting mates or fending off competitors, they can also be costly and influenced by the environment and genetic variation. The eyestalks of the stalk-eyed fly, Teleopsis dalmanni, are of interest because eyestalk length is the target of both intra- and intersexual selection and is also reduced by loci on a highly-divergent sex ratio X chromosome (XSR), a meiotic driver accounting for up to 30% of wild X chromosomes. Male stalk-eyed flies fight to control access to females and over food using a combination of low-intensity displays and highintensity physical fights. We staged, filmed, and scored contests between pairs of eyespan-matched males to evaluate whether X chromosome type impacts the behavior and outcome of aggressive interactions. While our results broadly match expectations from previous studies, we found that XSR males used more high-intensity behaviors than males carrying a non-driving, standard X chromosome (XST), particularly when their eyestalks were of similar size or smaller than their opponents. Additionally, we found that when XSR males use high-intensity behaviors, they win more bouts than when they use low-intensity behaviors. Taken together, these results suggest that XSR impacts male aggressive behavior to compensate for the shorter eyestalks of XSR males and may help to explain how this selfish chromosome is maintained. [ABSTRACT FROM AUTHOR]

This paper is concerned with prescribed-time control for spacecraft formation flying. Firstly, based on Lie group SE(3), the coupled 6-degrees-of-freedom kinematics and dynamics for leader and followers are modelled. Then, by using exponential coordinates of SE(3), the relative configuration between leader and followers is expressed and its linearised model is also given. Finally, by constructing a finite-time escape function and using the properties of parametric Lyapunov function, a novel time-varying high-gain controller is developed such that the spacecraft formation flying can be achieved within a finite time that can be explicitly prescribed. As a further result, the observer-based controller is also proposed for the same problem. Numerical simulations verify the effectiveness of the proposed approach. [ABSTRACT FROM AUTHOR]

ABSTRACT The unidirectional multilevel rectifier has immense potential in high‐power medium‐voltage industrial applications. This paper proposes a multiobjective control method based on the combination of optimal‐vector‐pair modulated model predictive control (OVP‐M2PC) and a phase‐shifted modulation strategy for a unidirectional five‐level rectifier. This strategy takes both of the inductor current tracking and capacitor voltage balancing as the control objectives. First, an optimal vector pair is determined based on the AC‐side current variation rate, and then, through combining the calculated action duration of OVP with carrier phase‐shifted modulation, inductor current tracking is achieved. Second, capacitor voltage balancing control is carried out by compensating for the duty cycles which also decouples the input current tracking and output capacitor voltage balance control. Compared with traditional FCS‐MPC, under the proposed control strategy, complex cost function calculation and weighting factor tuning are not required, which much reduced computational burden. Multiobjective control is achieved with better performance in both steady‐state and dynamic conditions. The superiority of the proposed strategy over traditional FCS‐MPC are validated through simulations and hardware experiments. [ABSTRACT FROM AUTHOR]

Animals rely on compensatory actions to maintain stability and navigate their environment efficiently. These actions depend on global visual motion cues known as optic-flow. While the optomotor response has been the traditional focus for studying optic-flow compensation in insects, its simplicity has been insufficient to determine the role of the intricate optic-flow processing network involved in visual course control. Here, we reveal a series of course control behaviours in Drosophila and link them to specific neural circuits. We show that bilateral electrical coupling of optic-flow-sensitive neurons in the fly's lobula plate are required for a proper course control. This electrical interaction works alongside chemical synapses within the HS-H2 network to control the dynamics and direction of turning behaviours. Our findings reveal how insects use bilateral motion cues for navigation, assigning a new functional significance to the HS-H2 network and suggesting a previously unknown role for gap junctions in non-linear operations. The circuitry underpinning visuomotor behaviors in flies is poorly understood. Here, the authors show that the underlying computations require bilateral electrical coupling to control turning dynamics and direction for effective course control. [ABSTRACT FROM AUTHOR]

Simple Summary: This study investigated species diversity and seasonal abundance of biting flies, specifically Stomoxyinae and tabanid flies, which are important pests capable of spreading diseases to animals, on a beef cattle and a buffalo farm in Nakhon Si Thammarat province, southern Thailand. Over the course of a one-year study (December 2020 to November 2021), 1912 biting flies were collected and identified, comprising seven Stomoxyinae and nine tabanid species. The five most predominant species were Tabanus megalops, Haematobia irritans exigua, Stomoxys calcitrans, Stomoxys indicus, and Stomoxys uruma. A higher number of flies were collected from the beef cattle farm compared to the buffalo farm, with most species peaking during the rainy season, except for H. i. exigua, which was more prevalent during the dry season. Temperature, relative humidity, and rainfall influenced fly density, revealing different patterns depending on the species. This study provides important information that is crucial for designing effective fly control strategies to reduce the impact of these flies on livestock health. This study investigated species diversity and seasonal abundance of Stomoxyinae and tabanid flies, which are significant pests and vectors of animal pathogens, on a beef cattle and a buffalo farm in Nakhon Si Thammarat province, southern Thailand. During a one-year period from December 2020 to November 2021, flies were collected using Nzi traps from 6 a.m. to 6 p.m. over three consecutive days each month, resulting in the capture of 1912 biting flies, representing seven Stomoxyinae and nine tabanid species. The five most prevalent species were Tabanus megalops, Haematobia irritans exigua, Stomoxys calcitrans, Stomoxys indicus, and Stomoxys uruma. Fly density was notably higher on the beef cattle farm compared to the buffalo farm, with most species peaking during the rainy season, except for H. i. exigua, which was more abundant during the dry season. This study also examined the influence of temperature, relative humidity, and rainfall on fly density, revealing species-specific patterns. These findings offer updated insights into species diversity and seasonal trends, providing critical baseline data essential for the development of effective control strategies aimed at mitigating the impact of these flies on livestock health. [ABSTRACT FROM AUTHOR]

Simple Summary: Biting hematophagous dipterans are responsible for painful bites and blood spoliation; they induce behavioral modifications, anemia, and significant production losses in cattle. A feedlot of 100 feeder cattle would register a total loss of USD 16,000 within 5 months, which appears to be an unexpectedly huge loss caused by dipterans. Investing part of this money in fly control would probably be beneficial. In addition to blood pathogen transmission, insects of the order Diptera affect livestock through visual and contact harassment; blood-feeders are responsible for painful bites and blood despoliation, generating behavioral modifications, anemia, and production losses. Knowledge of their economic impact is a basis for cost-effective control. Here, we measured the global impact of diptera insects by comparing two batches of six feeder cattle, one in the open air and the other protected by a mosquito net. The analytical data were insect density in the open air and, for feeder cattle, tail flick counts, hematocrit values (Ht), feed intake, feed conversion ratio (FCR), and live body weight gain (LBWG). Over a period of five months, the results showed significant losses in the LBWG of cattle exposed to insects, estimated at 8.0 ± 1.5 kg/month [2.7; 13.3], with a total loss reaching 40.0 ± 5.5 kg/head. Main diurnal insects were Stomoxys spp. and Musca crassirostris. There was a strong correlation between fly density and diurnal tail flicks. Night trapping and tail flicks showed a potentially important role of mosquitoes to be further explored. The Ht levels of exposed animals were 3–4% lower than those of controls. FCRs indicated that exposed animals needed 33% more dry matter intake/kg of LBWG. An economic assessment showed that dipterans were responsible for a 10–11% loss in LBWG during the main growing period of feeder cattle (10–15 months). A feedlot of 100 calves would register a total loss of USD 16,000 within 5 months, which appears to be an unexpectedly huge loss caused by dipterans. Investing part of this money into fly control would probably be beneficial. [ABSTRACT FROM AUTHOR]

Background/Objectives: The gonadotropins luteinizing hormone (LH) and follicle-stimulating hormone (FSH) and their receptors are major regulators of reproduction in mammals and are absent in insects. We previously established transgenic Drosophila lines expressing a constitutively active human LH receptor variant (LHRD578Y) and the wild-type receptor (LHRwt; inactive in the absence of an agonist). That study showed that ubiquitously expression of LHRD578Y—but not of LHRwt—resulted in pupal lethality, and targeted expression in midline cells resulted in thorax/bristles defects. To further study the Drosophila model for an in vivo drug screening platform, we investigated here whether expressing LHRD578Y in the fly gonads alters reproduction, as shown in a transgenic mice model. Methods: The receptor was expressed in somatic cells of the gonads using the tissue-specific traffic jam-Gal4 driver. Western blot analysis confirmed receptor expression in the ovaries. Results: A fecundity assay indicated that the ectopic expression of LHRD578Y resulted in a decrease in egg laying compared to control flies carrying, but not expressing the transgene (~40% decrease in two independent fly lines, p < 0.001). No significant reduction in the number of laid eggs was seen in flies expressing the LHRWT (<10% decrease compared to non-driven flies, p > 0.05). The decreased egg laying demonstrates a phenotype of the active receptor in the fly gonads, the prime target organs of the gonadotropins in mammals. We suggest that this versatile Drosophila model can be used for the pharmacological search for gonadotropin modulators. Conclusions: This is expected to provide: (a) new mimetic drug candidates (receptor-agonists/signaling-activators) for assisted reproduction treatment, (b) blockers for potential fertility regulation, and (c) leads relevant for the purpose of managing extra gonadotropic reported activities. [ABSTRACT FROM AUTHOR]

Bacteria‐originated volatile molecules play a crucial role in chemical communications between insects, representing their promising application as odor bait traps in pest control. In this study, we investigated the behavioral preferences of the fruit fly Drosophila melanogaster (Diptera: Drosophilidae) towards the fermentation broth of seven gut‐associated bacteria using trap choice assays. All seven bacterial fermentations significantly attracted adults and larvae compared to the medium control. We assessed the effects of bacterial fermentations on bayberry attractiveness to fly adults, and found that the bayberries sprayed with fermentation broth of seven bacteria were all significantly more attractive to adults than the non‐sprayed, following in increased total egg numbers. We also compared the attractive effect of bacterial fermentations with a sugar‐vinegar mixture and a commercial odor‐bait. The commercial odor bait proved more enticing than the unconcentrated 5‐day fermentation broths. However, out of the seven bacteria, 64‐fold concentrated bacterial fermentation of Corynebacterium (Actinomycetota phylum) was significantly more attractive than commercial bait. Finally, we chemically identified the predominant compounds 2‐methylpropanal and acetaldehyde, which are likely responsible for the behavioral preference of fruit flies. Our findings provide a deeper understanding of how gut microbes affect insect behavior and offer a potential bacteria‐originated odor bait for fly control in the orchard. [ABSTRACT FROM AUTHOR]

Tsetse flies (Diptera: Glossinidae) are the cyclical vectors of human and animal trypanosomes. This viviparous insect develops and produces a single larva at 10-day intervals deposited in specific sites. In some species aggregation of larvae has been shown and seems to be mediated by both physical factors and volatile semiochemicals of larval origin. In this context, this study aims to identify chemicals emitted during the pupariation process in Glossina palpalis gambiensis. Volatile Organic Compounds (VOCs) emitted by larvae were identified using static headspace solid-phase microextraction and gas-chromatography mass-spectrometry (GC–MS) analysis. Electrophysiology and behavioural assays were performed on gravid females to confirm VOCs behavioural activity and attractiveness. GC–MS results revealed ten chemicals emitted during the pupariation process of G. p. gambiensis larvae. Among these chemicals, gravid females were shown to detect nine of them during coupled gas chromatography - electroantennographic detection tests. Behavioural assays highlighted two compounds were as attractive as pupae and one compound and a blend of four compounds were more attractive than pupae. Although the larval origin of some of them needs to be confirmed as they may also likely produced by micro-organisms, these compounds induced significant behavioural responses in the laboratory. Further experiments have to explore the biological activity and competitiveness of these compounds in the field. This work opens interesting opportunities for behavioural manipulation and control of tsetse flies. [ABSTRACT FROM AUTHOR]

The human GBA1 gene encodes lysosomal acid β-glucocerebrosidase, whose activity is deficient in Gaucher disease (GD). In Drosophila, there are two GBA1 orthologs, Gba1a and Gba1b, and Gba1b is the bona fide GCase encoding gene. Several fly lines with different deletions in the Gba1b were studied in the past. However, since most GD-associated GBA1 mutations are point mutations, we created missense mutations homologous to the two most common GD mutations: the mild N370S mutation (D415S in Drosophila) and the severe L444P mutation (L494P in Drosophila), using the CRISPR-Cas9 technology. Flies homozygous for the D415S mutation (dubbed D370S hereafter) presented low GCase activity and substrate accumulation, which led to lysosomal defects, activation of the Unfolded Protein Response (UPR), inflammation/neuroinflammation, and neurodegeneration along with earlier death compared to control flies. Surprisingly, the L494P (called L444P hereafter) flies presented higher GCase activity with fewer lysosomal defects and milder disease in comparison to that presented by the D370S homozygous flies. Treatment with ambroxol had a limited effect on all homozygous fly lines tested. Overall, our results underscore the differences between the fly and human GCase enzymes, as evidenced by the distinct phenotypic outcomes of mutations in flies compared to those observed in human GD patients. [ABSTRACT FROM AUTHOR]

The house fly, Musca domestica, is a cosmopolitan species known for its pestiferous nature and potential to mechanically vector numerous human and animal pathogens. Control of adult house flies often relies on insecticides formulated into food baits. However, due to the overuse of these baits, insecticide resistance has developed to all insecticide classes currently registered for use in the United States. Field populations of house flies have developed resistance to imidacloprid, the most widely used neonicotinoid insecticide for fly control, through both physiological and behavioral resistance mechanisms. In the current study, we conducted a comprehensive analysis of the inheritance and dominance of behavioral resistance to imidacloprid in a lab‐selected behaviorally resistant house fly strain. Additionally, we conducted feeding preference assays to assess the feeding responses of genetic cross progeny to imidacloprid. Our results confirmed that behavioral resistance to imidacloprid is inherited as a polygenic trait, though it is inherited differently between male and female flies. We also demonstrated that feeding preference assays can be instrumental in future genetic inheritance studies as they provide direct insight into the behavior of different strains under controlled conditions that reveal, interactions between the organism and the insecticide. The findings of this study carry significant implications for pest management and underscore the need for integrated pest control approaches that consider genetic and ecological factors contributing to resistance. [ABSTRACT FROM AUTHOR]

BACKGROUND: The olive fruit fly (OFF), Bactrocera oleae (Rossi), is the main insect pest of olive trees worldwide. Legislation limits to the use of some synthetic larvicidal insecticides is leading to the development of new control options for preventive control of adult flies. In the present study, the biological activity of four short‐chain aliphatic aldehydes, namely hexanal, (E)‐2‐hexenal, heptanal and (E)‐2‐heptenal, previously reported as repellents to the OFF adults was investigated. RESULTS: Electroantennography (EAG) recordings showed that antennae of OFF males and females are able to perceive the test compounds in a wide range of doses. In field trapping experiments, reservoir‐type polypropylene (PP) membrane dispensers loaded with individual compounds did not elicit a significant attraction of OFF males and females. On the contrary, a significant reduction of male catches was noticed when sex pheromone dispensers and PP membrane dispensers, loaded with one of the test compounds, were applied on the same white sticky traps ≈20 cm apart. Likewise, male and female catches in yellow sticky traps baited with ammonium carbonate (AC) dispensers as food attractant were significantly reduced by the presence of PP membrane dispensers of individual aliphatic aldehydes on the same traps. In small plots control trials, solid formulations of the four aldehydes into a bentonite clay support induced a significant reduction of the OFF active infestation mainly when C6 and C7 aldehyde‐activated bentonites were used. CONCLUSION: Short‐chain aliphatic aldehydes showed inhibitory effects on sex pheromone and food attractant‐mediated attraction of OFF. Results of field trials suggest potential of short‐chain aliphatic aldehydes to develop new semiochemical‐based OFF control options. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann), one of the most important invasive pests of fresh fruits and vegetables from the coastal Mediterranean habitats, is expanding its current geographic distribution to cooler more temperate areas of Europe. Every year since 2010 the fly is detected in the area of Vienna, Austria. However, whether it can establish permanent populations is not known. In this current paper, the capacity of C. capitata to overwinter in Vienna, Austria (48.1° northern latitude) was studied over 2 consecutive winter seasons (2020–2022). Overwintering trials with different life stages (larva, pupa, and adult) of C. capitata were performed in the open field and in the protected environment of a basement without a heating system. Control flies were kept under constant conditions in a climate chamber (25 °C, 60% RH, 14:10 L:D). Our data showed that no life stage of the Mediterranean fruit fly was able to survive the Austrian winter in the open field. However, in the protected environment C. capitata outlived the winter months in all studied life stages at least in small numbers and several surviving females were able to lay eggs at the time of the following fruiting season. Implications of these findings for the ongoing geographic range expansion of the pest in temperate European countries are discussed. [ABSTRACT FROM AUTHOR]

A significant number of microorganisms inhabit the intestinal tract or the body surface of insects. While the majority of research on insect microbiome interaction has mainly focused on bacteria, of late multiple studies have been acknowledging the importance of fungi and have started reporting the fungal communities as well. In this study, high-throughput sequencing was used to compare the diversity of intestinal fungi in Delia antiqua (Diptera: Anthomyiidae) at different growth stages, and effect of differential fungi between adjacent life stages on the growth and development of D. antiqua was investigated. The results showed that there were significant differences in the α and β diversity of gut fungal communities between two adjacent growth stages. Among the dominant fungi, genera Penicillium and Meyerozyma and family Cordycipitaceae had higher abundances. Cordycipitaceae was mainly enriched in the pupal and adult (male and female) stages, Penicillium was mainly enriched in the pupal, 2nd instar and 3rd instar larval stages, and Meyerozyma was enriched in the pupal stage. Only three fungal species were found to differ between two adjacent growth stages. These three fungal species including Fusarium oxysporum, Meyerozyma guilliermondii and Penicillium roqueforti generally inhibited the growth and development of D. antiqua, with only P. roqueforti promoting the growth and development of female insects. This study will provide theoretical support for the search for new pathogenic microorganisms for other fly pests control and the development of new biological control strategies for fly pests. Three fungal species were found to differ between two adjacent growth stages. These three fungal species including Fusarium oxysporum, Meyerozyma guilliermondii and Penicillium roqueforti generally inhibited the growth and development of D. antiqua, with only P. roqueforti promoting the growth and development of female insects. [ABSTRACT FROM AUTHOR]

The circadian rhythms are ubiquitous and confer an adaptive advantage to an individual/population by synchronising its daily activity to its surroundings. Circadian rhythms are controlled by self-sustained oscillator and they continue to oscillate in constant condition. Locomotor activity is one such robust self sustained oscillation. It is essential/crucial for an organism's survival such as feeding, mate choice, escape from the predator, etc. The present investigations were made to understand the effect of Mucuna pruriens and Glycyrrhiza glabra on locomotor behaviour of the Drosophila melanogaster (Oregon K strain) and vestigial wing mutant flies. Locomotor activity rhythms were assayed in D. melanogaster and vestigial wing mutant flies using Drosophila activity monitor and the amount of activity was measured. The results revealed that the Mucuna pruriens and Glycyrrhiza glabra treated flies showed increased activity when compared to control flies, between treated groups, higher concentration treated flies showed maximum activity than lower concentration treated flies. Statistical analysis showed that there is a significant difference in the locomotor activity among control and treated groups of D. melanogaster and vestigial wing mutant of Mucuna pruriens seed and Glycyrrhiza glabra root extract. [ABSTRACT FROM AUTHOR]

Purpose: To ensure the stability of the flying wing layout unmanned aerial vehicle (UAV) during flight, this paper uses the radial basis function neural network model to analyse the stability of the aforementioned aircraft. Design/methodology/approach: This paper uses a linear sliding mode control algorithm to analyse the stability of the UAV's attitude in a level flight state. In addition, a wind-resistant control algorithm based on the estimation of wind disturbance with a radial basis function neural network is proposed. Through the modelling of the flying wing layout UAV, the stability characteristics of a sample UAV are analysed based on the simulation data. The stability characteristics of the sample UAV are analysed based on the simulation data. Findings: The simulation results indicate that the UAV with a flying wing layout has a short fuselage, no tail with a horizontal stabilising surface and the aerodynamic focus of the fuselage and the centre of gravity is nearby, which is indicative of longitudinal static instability. In addition, the absence of a drogue tail and the reliance on ailerons and a swept-back angle for stability result in a lack of stability in the transverse direction, whereas the presence of stability in the transverse direction is observed. Originality/value: The analysis of the stability characteristics of the sample aircraft provides the foundation for the subsequent establishment of the control model for the flying wing layout UAV. [ABSTRACT FROM AUTHOR]

The environment surrounding invertebrates can influence the physiology of larval offspring. Dung beetles provide several significant ecological functions, including dung breakdown, fly control and nutrient cycling. Cattle diet influences the chemical and physical constituents of dung, of which pH is considered critical. Few studies have assessed this, though a pH of 6.3 is the lowest threshold for dung beetle reproduction. We investigated the effects of an introduced and widespread dung beetle (Onthophagus binodis) on cattle dung pH (7.3, 6.0 and 5.0) and pH on O. binodis reproduction, offspring phenotypic traits and development time. Dung beetle presence increased the Δ pH (more alkaline) within dung pads after 96 h. Dung beetles produced broods in dung with a pH of 5.0, though in fewer numbers compared with the other pH treatments. Larval development was delayed in pH 5.0 with an average of 50 days compared with 44 days in dung with pH 6, 7, and the control (7.3). Smaller broods (ellipsoid volume [mm3]) were produced in dung with a pH of 5.0 compared with pH 6.0 and 7.0, and offspring emerging from broods produced from dung with a pH of 6.0 were larger compared with the other pH treatments. Our results show that dung pH is important for brood production and progeny phenotypic traits of O. binodis, an agricultural ecosystem engineer and that there is no experimental evidence to support the suggestion that dung pH influences the provisioning of broods in this species. [ABSTRACT FROM AUTHOR]

Larvae of Hydrotaea are facultative predators of larvae of flies commonly found in poultry farms, chicken and pig waste, and decaying carcasses. One species, the black dump fly, Hydrotaea aenescens (Wiedemann, 1830), coexists with the housefly Musca domestica (Linnaeus, 1758) in these environments and has been employed in the biological control of the housefly in the USA and Germany. To assess the predatory efficacy of H. aenescens larvae on M. domestica larvae, we conducted controlled laboratory experiments in which we selected larvae of different sizes of the larvae and the densities of both predator and prey. We set up the experiment using adult flies from a poultry farm and reared the larvae they produced in our laboratory. Our findings indicate that second‐ and third‐instar H. aenescens larvae effectively suppressed populations of housefly larvae under laboratory conditions, especially when the latter was smaller or the same size as the former. Under these ideal experimental conditions, a single H. aenescens larva consumed up to 29 larvae of M. domestica, potentially leading to a complete eradication of the housefly population at predator–prey density levels of 1:1 and 1:4, but only when considering both the natural mortality rate of M. domestica larvae and the additional mortality caused by H. aenescens larvae. These results corroborate a pattern previously observed in other predatory larvae and have important implications for biological control and integrated pest management programs targeting M. domestica in poultry and swine farms across South America. [ABSTRACT FROM AUTHOR]

Infectious bovine keratoconjunctivitis (IBK) commonly known as pinkeye is a contagious non-fatal bacterial infection of the eye that has tremendous economic impact on the dairy industry. Nevertheless, it is a much-neglected dairy issue in Pakistan. The current study was aimed to find out the prevalence and risk factors associated with pinkeye infection in commercial dairy herds in Pakistan. This study recruited 10,573 cows in 20 commercial dairy herds holding ≥25 cows per herd. The cows were assessed based on eye lesions using a scale from 1 to 4, with 4 being the most severe of all. Culture of subconjunctival swab samples was also undertaken. The overall prevalence of pinkeye was 6.86% with Holstein-Friesian breed being the most affected (7.25%) followed by crossbred (6.89%) cattle. Cow hygiene and farm hygiene scores were indirectly related to the prevalence of pinkeye. Prevalence was also significantly (P<0.05) associated with seasons of the year, being highest in humid summer months while lowest in winter. The prevalence of pinkeye was significantly higher (<0.0001) in cows exposed to sunlight than unexposed ones. Cows on farms performing farm disinfection occasionally had 1.52 times higher odds of being diseased than cows on farms with once-a-week disinfection. Likewise, cows on farms using no fly control program had 2.8 times higher likelihood of pinkeye than those on farms having fly control program. In addition, prevalence of pinkeye was significantly higher (P<0.05) in cows with low body condition scores. It was concluded that Pinkeye is a significant health concern in commercial dairy herds in Pakistan and the risk factors influencing the prevalence of this disease may include cow hygiene, farm management practices, and the environment. [ABSTRACT FROM AUTHOR]

Monitoring of breeding success in wildlife is key for evaluating population trends and support conservation measures. However, it is challenging depending on breeding places or non-accessible habitat. In recent years, new technologies were applied to studies of bird biology, i.e. drones. UAV (drones) are available to study new aspects of bird ecology, which are not available using traditional methods. The Raven Corvus corax predominantly nests in upper tree canopies or on high cliffs. Hence, it is challenging to monitor it breeding success from the ground. Our study were conducted in forests in NE Poland, on the 77 arboreal Raven nests in 2021–2022. By using drones, we investigated (i) whether the classic data on breeding success from ground observations differ from those of nest inspections via drone, and (ii) to what extent the adult birds behave when the drone is used at the nest. The inspection from the ground failed to determine the contents of as many as 30% of the occupied nests. Inspections using a drone and those made from the ground yielded different results, as aerial assessment showed a higher number of the young (respectively 1.93 vs. 3.22 per successful brood). Broods with three young accounted for 6.5% of nests controlled from the ground and 23.3% using a drone, while broods with two young accounted for 33.8% and 10.4% of nests, respectively. During most of the inspected nests (65% of the cases), adult birds sat nearby in the tree tops and called. In 12% of the cases, the birds flew close to the nest, passing the drone at a distance of more than 20 m, and did not attempt to approach the device. In four cases, adult birds closed the drone at less than 10 m. A direct collision did not happen. The most vital reactions were recorded in several instances (only 4.2%) in which the birds were not near the nest when the inspection began but arrived during the inspection. Drone surveys have revealed previously un-described behavior of birds, alarmed at the examination of empty nests. In 50 nests, 65% of the cases, the Raven tolerated the drone's presence at the nest, although the birds showed concern and occasionally defensive reactions. It seems that the adverse reaction of birds is weaker when they associate the appearance of a drone with human presence. Key recommendations: control in teams of two - operator and observer, starting end landing in close vicinity of the nest tree, immediate interruption of flight if birds react, forbade flying directly over the nest. It is advisable to check the reaction of adult Ravens to drone inspections of nests with small nestlings. To sum up, our study showed that the drone inspections did not harm the adult birds but provides a more reliable data for reproduction than the observation from ground. [ABSTRACT FROM AUTHOR]

Background: Flies are acknowledged as vectors of diseases transmitted through mechanical means and represent a significant risk to human health. The study aimed to determine the prevalence of enteropathogens carried by flies in Pudong New Area to inform strategies for preventing and controlling flies. Methods: Samples were collected from various locations in the area using cage trapping techniques between April and November 2021, encompassing various habitats such as parks, residential areas, restaurants, and farmers' markets. The main fly species were identified using cryomicrography and taxonomic enumeration, with 20 samples per tube collected from different habitats. Twenty-five enteropathogens were screened using GI_Trial v3 TaqManTM microbial arrays. Results: A total of 3,875 flies were collected from 6,400 placements, resulting in an average fly density of 0.61 flies per cage. M. domestica were the most common species at 39.85%, followed by L. sericata at 16.57% and B. peregrina at 13.14%. Out of 189 samples, 93 tested positive for enteropathogens, with nine different pathogens being found. 12.70% of samples exclusively had parasites, a higher percentage than those with only bacteria or viruses. The study found that M. domestica had fewer enteropathogens than L. sericata and B. peregrina, which primarily harbored B. hominis instead of bacteria and viruses such as E. coli, Astrovirus, and Sapovirus. During spring testing, all three fly species exhibited low rates of detecting enteropathogens. M. domestica were found in residential areas with the highest number of pathogen species, totaling six. In contrast, L. sericata and B. peregrina were identified in farmers' markets with the highest number of pathogen species, totaling six and seven, respectively. Conclusions: Flies have the potential to serve as vectors for the transmission of enteropathogens, thereby posing a substantial risk to public health. [ABSTRACT FROM AUTHOR]

Human African trypanosomiasis (HAT), or sleeping sickness, continues to be a major threat to human health in 36 countries throughout sub-Saharan Africa with up to 60 million people at risk. Over the last decade, there have been several advances in this area, some of which are discussed in this overview. Due to the concerted efforts of several bodies, including better identification and treatment of cases and improved tsetse fly vector control, the number of cases of HAT has declined dramatically. The clinical heterogeneity of HAT has also been increasingly recognized, and the disease, while usually fatal if untreated or inadequately treated, does not always have a uniformly fatal outcome. Improved methods of HAT diagnosis have now been developed including rapid diagnostic tests. Novel drug treatment of HAT has also been developed, notably nifurtimox–eflornithine combination therapy (NECT) for late-stage Trypanosoma brucei gambiense , oral fexinidazole for early and the early component of the late-stage of T.b. gambiense , and the new oral compounds of the oxaborole group, which have shown considerable promise in field trials. Advances in HAT neuropathogenesis have been steady, though largely incremental, with a particular focus on the role of the blood–brain barrier in parasite entry into the central nervous system and the relevant importance of both innate and adaptive immunity. While the World Health Organization goal of elimination of HAT as a public health problem by 2020 has probably been achieved, it remains to be seen whether the second more ambitious goal of interruption of transmission of HAT by 2030 will be attained. [ABSTRACT FROM AUTHOR]

Wolbachia is a maternally inherited intracellular bacterium that is considered to be the most plentiful endosymbiont found in arthropods. It reproductively manipulates its host to increase the chances of being transmitted to the insect progeny; and it is currently used as a means of suppressing disease vector populations or controlling vector-borne diseases. Studies of the dissemination and prevalence of Wolbachia among its arthropod hosts are important for its possible use as a biological control agent. The molecular identification of Wolbachia relies on different primers sets due to Wolbachia strain variation. Here, we screened for the presence of Wolbachia in a broad range of Brachycera fly species (Diptera), collected from different regions of Iran, using nine genetic markers (wsp, ftsZ, fbpA, gatB, CoxA, gltA, GroEL dnaA, and 16s rRNA), for detecting, assessing the sensitivity of primers for detection, and phylogeny of this bacterium. The overall incidence of Wolbachia among 22 species from six families was 27.3%. The most commonly positive fly species were Pollenia sp. and Hydrotaea armipes. However, the bacterium was not found in the most medically important flies or in potential human disease vectors, including Musca domestica, Sarcophaga spp., Calliphora vicinia, Lucilia sericata, and Chrysomya albiceps. The primer sets of 16s rRNA with 53.0% and gatB with 52.0% were the most sensitive primers for detecting Wolbachia. Blast search, phylogenetic, and MLST analysis of the different locus sequences of Wolbachia show that all the six distantly related fly species likely belonging to supergroup A. Our study showed some primer sets generated false negatives in many of the samples, emphasizing the importance of using different loci in detecting Wolbachia. The study provides the groundwork for future studies of a Wolbachia-based program for control of flies. [ABSTRACT FROM AUTHOR]

Simple Summary: The genus Bactrocera contains invasive fruit fly species that lay eggs in and consume many important fruits and vegetables. Two tactics used to control these flies include the male annihilation and the sterile insect techniques. Bactrocera males are attracted to and feed on methyl eugenol, a chemical found in many plants. Baiting traps with a mixture of this chemical and an insecticide are used to annihilate males and, thereby, the pest population. The release of sterile males (achieved via irradiation) results in mating with wild females that results in inviable embryos. Feeding on ME (without insecticide) enhances the mating success of Bactrocera spp. males and suppresses their subsequent attraction to methyl eugenol + insecticide traps, enabling the simultaneous application of the male annihilation and the sterile insect techniques, which will boost the efficiency of control programs. However, methyl eugenol may pose human health risks, and this study demonstrated that feeding on an alternative and safer plant compound, β-caryophyllene, similarly led to the increased mating success of Bactrocera zonata and reduced their subsequent attraction to methyl-eugenol-baited traps. The peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae), is an economically important polyphagous quarantine pest of horticultural crops endemic to South and Southeast Asia. Methyl eugenol (ME), a naturally occurring phenylpropanoid, is a male attractant used to lure and (when mixed with an insecticide) annihilate the males from the wild population, a method of pest control termed the male annihilation technique (MAT). ME is reported to enhance the mating success of sterile males of Bactrocera spp., which is critical for enhancing the effectiveness of the sterile insect technique (SIT). The suppressed response of ME-treated males to ME-baited traps/devices allows the simultaneous application of the MAT and SIT, increasing the efficiency of area-wide integrated pest management (AW-IPM) programs. However, ME treatment in sterile males in SIT facilities is logistically difficult. β-caryophyllene (BCP) is a widely occurring, safer plant compound and is considered suitable for treating males in SIT facilities. Here, we demonstrate that BCP feeding enhanced B. zonata male mating success to the same extent as ME feeding. Feeding on BCP suppressed the male's subsequent attraction to ME-baited traps, but not to the same degree as feeding on ME. The results are discussed and BCP is suggested as an alternative to ME for the concurrent use of the MAT and SIT. [ABSTRACT FROM AUTHOR]

Werner syndrome (WS) is a rare genetic disease in humans, caused by mutations in the WRN gene that encodes a protein containing helicase and exonuclease domains. WS is characterized by symptoms of accelerated aging in multiple tissues and organs, involving increased risk of cancer, heart failure, and metabolic dysfunction. These conditions ultimately lead to the premature mortality of patients with WS. In this study, using the null mutant flies (WRNexo Δ ) for the gene WRNexo (CG7670), homologous to the exonuclease domain of WRN in humans, we examined how diets affect the lifespan, stress resistance, and sleep/wake patterns of a Drosophila model of WS. We observed that dietary restriction (DR), one of the most robust nongenetic interventions to extend lifespan in animal models, failed to extend the lifespan of WRNexo Δ mutant flies and even had a detrimental effect in females. Interestingly, the mean lifespan of WRNexo Δ mutant flies was not reduced on a protein-rich diet compared to that of wild-type (WT) flies. Compared to WT control flies, the mutant flies also exhibited altered responses to DR in their resistance to starvation and oxidative stress, as well as changes in sleep/wake patterns. These findings show that the WRN protein is necessary for mediating the effects of DR and suggest that the exonuclease domain of WRN plays an important role in metabolism in addition to its primary role in DNA-repair and genome stability. [ABSTRACT FROM AUTHOR]

In this study, a novel control framework for the collaboration of an aerial robot and a ground vehicle that is connected via a taut tether is proposed. The framework is based on a leader-follower paradigm. The leader follows a desired trajectory while the motion of the follower is controlled by an admittance controller using an extended state observer to estimate the tether force. Additionally, a velocity estimator is also incorporated to accurately assess the leader's velocity. An essential feature of our system is its adaptability, enabling role switching between the robots when needed. Furthermore, the synchronization performance of the robots is evaluated through quantitative analysis using the RMS position error metric and circular variance metric that is used in the mirror game to measure synchronization in human interactions. Similar to human-human interactions, we have observed that robotic agents can effectively guide one another based solely on interaction cues and adapt as needed. Our findings demonstrate the remarkable ability of robots to closely follow each other using a velocity estimation approach, without the reliance on sensors. This work contributes to the field by advancing robotic collaboration capabilities under limited sensor conditions, capitalizing on the inherent sensing capabilities of the robots, and providing a versatile platform for the study of cooperative behaviors in artificial systems. [ABSTRACT FROM AUTHOR]

Head grooming in Drosophila consists of repeated sweeps of the legs across the head, comprising regular cycles. We used the GAL4-UAS system to study the effects of overexpressing shibirets1 and of Adar knockdown via RNA interference, on the period of head-grooming cycles in Drosophila. Overexpressing shibirets1 interferes with synaptic vesicle recycling and thus with cell communication, while Adar knockdown reduces RNA editing of neuronal transcripts for a large number of genes. All transgenic flies and their controls were tested at 22° to avoid temperature effects; in wild type, cycle frequency varied with temperature with a Q10 of 1.3. Two experiments were performed with transgenic shibirets1: (1) each fly was heat-shocked for 10 min at 30° immediately before testing at 22° and (2) flies were not heat shocked. In both experiments, cycle period was increased when shibirets1 was overexpressed in all neurons, but was not increased when shibirets1 was overexpressed in motoneurons alone. We hypothesize that grooming cycles in flies overexpressing shibirets1 are lengthened because of synaptic impairment in neural circuits that control head-grooming cycles. In flies with constitutive, pan-neuronal Adar knockdown, cycle period was more variable within individuals, but mean cycle period was not significantly altered. We conclude that RNA editing is essential for the maintenance of within-individual stereotypy of head-grooming cycles. [ABSTRACT FROM AUTHOR]

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CO2 anesthesia is the most common method for immobilizing Drosophila for research purposes. But CO2 exposure has consequences—it can impact fertility, behavior, morphogenesis, and cytoskeletal dynamics. In this respect, Drosophila is an outstanding model for studying the impact of CO2 exposure on tissues. In this study we explored the response of intracellular pH (pHi) to a one-minute CO2 pulse using a genetically encoded, ubiquitously expressed pH sensor, tpHusion, to monitor pHi within a live, intact, whole fly. We compared wild-type flies to flies lacking Imaginal disc growth factors (Idgfs), which are chitinase-like proteins that facilitate developmental processes and the innate immune response. Morphogenetic and cytoskeletal defects in Idgf-null flies are enhanced after CO2 exposure. We found that pHi drops sharply within seconds of the beginning of a CO2 pulse and recovers over several minutes. The initial profile was nearly identical in control and Idgf-null flies but diverged as the pHi returned to normal. This study demonstrates the feasibility of monitoring pH in live adult Drosophila. Studies exploring pH homeostasis are important for understanding human pathologies associated with pH dysregulation. [ABSTRACT FROM AUTHOR]

Sustainable and effective means to control flying insect vectors are critically needed, especially with widespread insecticide resistance and global climate change. Understanding and controlling vectors requires accurate information about their movement and activity, which is often lacking. The Photonic Fence (PF) is an optical system that uses machine vision, infrared light, and lasers to identify, track, and interdict vectors in flight. The PF examines an insect's outline, flight speed, and other flight parameters and if these match those of a targeted vector species, then a low-power, retina-safe laser kills it. We report on proof-of-concept tests of a large, field-sized PF (30 mL × 3 mH) conducted with Aedes aegypti, a mosquito that transmits dangerous arboviruses, and Diaphorina citri, a psyllid which transmits the fatal huanglongbing disease of citrus. In tests with the laser engaged, < 1% and 3% of A. aegypti and D. citri, respectfully, were recovered versus a 38% and 19% recovery when the lacer was silenced. The PF tracked, but did not intercept the orchid bee, Euglossa dilemma. The system effectively intercepted flying vectors, but not bees, at a distance of 30 m, heralding the use of photonic energy, rather than chemicals, to control flying vectors. [ABSTRACT FROM AUTHOR]

Simple Summary: House flies (Musca domestica) are a significant nuisance and vector species. Currently, few technologies are available to adequately control their populations, and insecticide resistance threatens the efficacy of some approaches currently utilized. Transfluthrin is a promising volatile pyrethroid that has been successful in controlling mosquitoes and other pest insects, offering the potential as an alternative or future chemical control tool for the abatement of diverse arthropod pests. For this study, we tested whether transfluthrin could prevent fly capture at an attractant source compared to the control when placed on the exterior of the attractant device. Our results indicate that transfluthrin significantly reduced fly capture for both a pyrethroid-susceptible and pyrethroid-resistant strain of house fly compared to the untreated controls. These results indicate the potential of using transfluthrin in future integrated pest management programs for the control of house flies. House flies are notoriously difficult to control, owing to their tendency to live in close relationships with humans and their livestock, and their rapid development of resistance to chemical controls. With this in mind, we explored an alternative chemical control, a spatial repellent to deter Musca domestica L. from points we wanted to protect (i.e., a baited trap). Our results demonstrated that the synthetic spatial repellent, transfluthrin, is effective in preventing M. domestica adults from entering protected traps for both a susceptible strain (CAR21) and a field-acquired permethrin-resistant strain (WHF; 24 h LD50 resistance ratio of 150), comprising 22% and 28% of the total number of flies collected, respectively. These results are promising and demonstrate that transfluthrin can be an effective spatial repellent to protect points of interest where needed. [ABSTRACT FROM AUTHOR]

Simple Summary: Stable flies, known for inflicting painful bites on livestock, cause substantial economic losses worldwide by hindering animal feeding and potentially transmitting diseases. Current chemical control methods face challenges of resistance and environmental risks. To address this, an integrated pest management approach is proposed and tested in a sanctuary in western Spain. This strategy combines cultural practices, animal protection, the release of natural enemies targeting immature stages (predatory mites and two species of wasp parasitoids), and a specialized trapping system for adults. The study highlights the use of this holistic strategy toward a sustainable and environmentally friendly method for stable fly control. This approach aims to mitigate economic losses and reduce reliance on traditional insecticides, contributing to improved livestock well-being and ecosystem health. Stable flies, Stomoxys calcitrans, stand as formidable pests with a global impact, inflicting significant economic losses on the livestock sector. Larval development occurs in diverse substrates, including decomposing plant material and manure, while emerged adults pose a threat through blood-feeding on both animals and humans. Conventional chemical control methods, predominantly reliant on insecticides, not only pose environmental risks but also face challenges of resistance among stable fly populations. To address this pressing issue, we propose an integrated pest management (IPM) strategy for stable fly control. This approach involved a combination of sanitary-cultural practices, animal protection, the release of natural enemies targeting immature stages, and a specialized trapping system for adults. The Stomoxycc® trap, designed for mass trapping of adult Stomoxys, was employed alongside the release of the predatory mite Macrocheles robustulus and two wasp parasitoids, Spalangia cameroni and Muscidifurax raptor (under the commercial brands Biomite® and Biowasp®) on animal bedding as a key component of this IPM strategy. The implementation of this initiative has been undertaken at a significant sanctuary for donkeys and mules in western Spain. In this publication, we present the application and results of the IPM strategy utilized and provide insights into its use as a sustainable and environmentally friendly option for controlling stable fly populations. [ABSTRACT FROM AUTHOR]

The Hessian fly, Mayetiola destructor (Say), is one of the most important insect pest plaguing wheat (Triticum aestivum , L) producers across the United States and around the world. Genetic resistance is the stalwart for control of Hessian fly. However, new genotypes (biotypes) arise in deployment of wheat containing resistance genes, so field populations must be evaluated periodically to provide information on the efficacy of those deployed genes. Louisiana (LA), with its diverse agricultural landscape, is not exempt from the challenges posed by this destructive pest. We previously documented the resistance response of wheat lines harboring Hessian fly resistance (H) genes against field populations collected in 2008 from across the southeastern United States, including Iberville Parish, LA. In the spring of 2023, we reevaluated the resistance response of 27 H genes from the field populations collected from Iberville Parish, LA, and compared the results with those observed in 2008. Sixteen H genes showed comparable resistance to the field populations from both years. While 3 of the H genes, H11 , H23 , and H24 , showed a significant decrease in resistance, 2 genes, H16 and H31 , had marked increase in resistance. Furthermore, 6 additional H genes were evaluated in 2023, with 4 showing >70% resistance. Our results clearly identify a total of 20 H genes that are moderate to highly effective against the 2023 Hessian fly population from Iberville Parish, LA. The resistance response documented in this study offers valuable information to wheat breeders in the region for effective management of this insect pest. [ABSTRACT FROM AUTHOR]

Flying cars offer huge advantages due to their deformable structure, which can adapt to external environments and mission requirements. They represent a novel system that can realize vertical takeoff and landing. However, the structure of a flying car is complicated, placing higher requirements on modeling accuracy and control effectiveness. Thus, in this paper, a dynamic model of a flying car is proposed by combining a car body, motor, and propellers. Then, a double-loop controller based on active disturbance rejection control is proposed to accurately control its flight altitude. Utilizing the extended state observer, external wind and other disturbances are regarded as an extended state, which can be dynamically observed and compensated to significantly improve tracking accuracy. The effectiveness of the proposed controller is validated through detailed simulations and flight experiments. The proposed controller significantly improves control accuracy and disturbance rejection capability. [ABSTRACT FROM AUTHOR]

Synanthropic filth flies are common where sanitation is poor and fecal wastes are accessible to them. These flies have been proposed as mechanical vectors for the localized transport of fecal microbes including antimicrobial resistant (AMR) organisms and associated antimicrobial resistance genes (ARGs), increasing exposure risks. We evaluated whether an onsite sanitation intervention in Maputo, Mozambique reduced the concentration of enteric bacteria and the frequency of detection of ARGs carried by flies collected in household compounds of low-income neighborhoods. Additionally, we assessed the phenotypic resistance profile of Enterobacteriaceae isolates recovered from flies during the pre-intervention phase. After fly enumeration at study compounds, quantitative polymerase chain reaction was used to quantify an enteric 16S rRNA gene (i.e., specific to a cluster of phylotypes corresponding to 5% of the human fecal microflora), 28 ARGs, and Kirby Bauer Disk Diffusion of Enterobacteriaceae isolates was utilized to assess resistance to eleven clinically relevant antibiotics. The intervention was associated with a 1.5 log10 reduction (95% confidence interval: -0.73, -2.3) in the concentration of the enteric 16S gene and a 31% reduction (adjusted prevalence ratio = 0.69, [0.52, 0.92]) in the mean number of ARGs per fly compared to a control group with poor sanitation. This protective effect was consistent across the six ARG classes that we detected. Enterobacteriaceae isolates–only from the pre-intervention phase–were resistant to a mean of 3.4 antibiotics out of the eleven assessed. Improving onsite sanitation infrastructure in low-income informal settlements may help reduce fly-mediated transmission of enteric bacteria and the ARGs carried by them. [ABSTRACT FROM AUTHOR]

Several conventional and recently available tools are available for an integrated control of European rabbits in Australia. We quantified the impact of the release of rabbit haemorrhagic disease virus K5 (RHDV K5, hereafter K5) and pindone (2‐pivalyl‐1,3‐indandione) baiting at 13 sites within Cudlee Creek fire scar in the Adelaide Hills, South Australia. K5 release was followed by pindone baiting between December 2021 and March 2022; the application of both control methods followed industry best practice. We counted rabbits using spotlights before and after the application of both control methods. Fly samples and livers from dead rabbits were collected to track K5 transmission within and between sites, and to detect the natural circulation of rabbit haemorrhagic disease virus 2 (RHDV2). K5 release had minimal impact on rabbit populations, with treated populations increasing by a mean of 65.5% at 14 days post‐release and 27.9% at 77 days post‐K5 release across all sites, comparable to the changes at control sites. K5 detection in flies up to 77 days post its release, and its detection in rabbit livers, demonstrates that it can survive and transmit in the environment for prolonged periods and that it can lethally infect some rabbits. This limited impact of K5 is consistent with previous studies and may be explained by pre‐existing RHDV/RHDV2 immunity in the target populations or the presence of young rabbits with natural innate RHDV immunity. The detection of K5 in flies from control sites demonstrates that it was vectored beyond its release location. A reduction in rabbit counts post‐pindone baiting was observed at most treatment sites, with a mean population reduction of 36.6% across all sites. Landholders need to carefully and strategically plan their integrated rabbit control programmes. Not all combinations of controls, even if theoretically logical, achieve meaningful outcomes for rabbit management. [ABSTRACT FROM AUTHOR]

More than 25 years ago, Donald Stokes argued that we must move beyond the false dichotomy of basic or applied research and suggested that when considering a program of scientific research it is important to ask whether (i) the work is motivated by use and (ii) if there is a search for fundamental understanding. Giving yes/no answers to these questions allows us to characterize research more fully, replacing the "or" of "basic or applied" by a richer understanding of the process of science. Stokes proposed that research that was motivated by a consideration of use and sought fundamental understanding be called research in Pasteur's Quadrant. One advantage of such work is that the search for fundamental understanding means that the problem-solving tools are more likely to be transferrable. After reviewing Stokes's formulation of research, I illustrate it with examples from the control of tephritid flies and the use of insect parasitoids for biological control. Thinking about one's work within Stokes's framework has many advantages for individual scientists, including guidance for journal selection, how to organize and conclude papers and seminars, and the "elevator speech." Furthermore, since research in Pasteur's Quadrant has the characteristic of simultaneously increasing our understanding of how the world works and improving applications, it will more likely benefit the community of pest scientists. [ABSTRACT FROM AUTHOR]

In this article, a generalized control scheme is proposed to extend the operating range of three-phase hybrid cascaded H-bridge (HCHB) inverters into various voltage levels without necessitating alterations to the core structure or the integration of additional H-bridge submodules. This study addresses a critical challenge related to capacitor voltage drift at various modulation indices and power factors, which is a serious impediment to various applications. To overcome this challenge, a novel balancing control scheme has been developed based on the injection of two independent offset voltages to simultaneously control the DC-link and flying capacitors. A distinctive aspect of the proposed technique involves adjusting the common reference voltage to attain the nearest level in the same cluster, thereby mitigating the insufficiency of redundant switching states. The effectiveness of the proposed technique to regulate the capacitor voltages at various operating conditions has been verified through simulation and experimental results. [ABSTRACT FROM AUTHOR]

The paper presents the results of an experimental study of the influence of distributed engines mounted downstream of the trailing edge on the structure of a separated flow around a trapezoidal model of a flying wing in a subsonic wind tunnel. Visualization patterns of the near-wall flow on the leeward side of the model are obtained in the modes of blocked engines and for the rotational speed of the impeller of 32800 rpm in the range of angles of attack of the wing . The studies also take into account the location of distributed propulsion relative to the level of the trailing edge, where the axis of rotation of the engine impeller coincided with the continuation of the wing chord line or is higher than that. The possibility of controlling a separated flow by using sources of stationary disturbances in the form of cones and ribs locally mounted at singular points on the wing surface is studied. [ABSTRACT FROM AUTHOR]

Stress caused by predator exposure can lead to various behavioural, physiological, stoichiometric, and biochemical changes in prey. Prior research has shown that growth under predation stress can cause the development of a diabetes-like biochemical phenotype in fruit flies. Exposure to predator risk during larval development decreases flies' walking activity, improving their antipredator strategies. However, it is unclear which elements of walking behaviour make flies less conspicuous to predators. This study shows that fruit flies (N = 729) grown with spiders walk shorter distances, accelerate faster and spend more time in a state of motion without movement (i.e., stomping in place) than control flies (N = 839). Under predation risk, adult flies grown with spiders survived better than control flies. We suggest that motions without movement may resemble sickness behaviour for predators, which we propose as the main reason for their better survival under direct exposure to predator attacks. [ABSTRACT FROM AUTHOR]

This paper introduces a cost-effective control method to enhance the low voltage ride-through (LVRT) capability and smooth the output power of a three-phase multilevel flying capacitor inverter (FCI) in wind turbine-based permanent magnet synchronous generator (PMSG). The proposed approach utilizes the energy storage capability of flying capacitors to mitigate wind power fluctuations and address short-duration outages and deep voltage sags. Additionally, a nonlinear controller based Lyapunov theory is developed to regulate capacitor voltages, improve power factors, and balance DC-link voltage. Numerical simulations are conducted in MATLAB/SimPower systems environment to validate the effectiveness of this comprehensive control strategy across different grid operation scenarios. [ABSTRACT FROM AUTHOR]

Abstract Background Stomoxys calcitrans, the stable fly, occurs in pig producing countries worldwide. While in cattle the impact of this blood sucking insect is quite well described, its role in pig production is poorly investigated. Here we describe a case of a massive stable fly overpopulation in the gestation unit of a piglet producing farm in Austria that resulted in bleeding skin lesions in bitten sows. Case Presentation In October 2021, the responsible herd veterinarian of the case farm reported of sows in the gestation area presenting with bloody crusts on the whole skin surface of the body and of bleeding skin lesions. 33/55 sows were affected by moderate to severe skin lesions. Reproductive performance decreased during the time of massive stable fly overpopulation. Sows in the gestation unit showed defensive behaviour and at a certain time point resigned and accepted being bitten by stable flies. After controlling the fly population, reproductive performance improved and even exceeded the performance before the massive overgrowth of the stable fly population. Conclusions Stable flies are a serious harm to pigs and should be kept in mind for improved animal health and welfare. Knowledge about the determination of Stomoxys calcitrans and early recognition of an increasing stable fly population in pig farming systems followed by proper insect control measures have to be performed to reduce losses caused by this harming insect.